LOCUS       NM_001258306            1290 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens coiled-coil domain containing 74A (CCDC74A),
            transcript variant 4, mRNA.
ACCESSION   NM_001258306
VERSION     NM_001258306.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Zhou,H., Zheng,T., Wang,T., Li,Q., Wang,F., Liang,X., Chen,J. and
            Teng,J.
  TITLE     CCDC74A/B are K-fiber crosslinkers required for chromosomal
            alignment
  JOURNAL   BMC Biol 17 (1), 73 (2019)
   PUBMED   31521166
  REMARK    GeneRIF: The authors characterized CCDC74A and CCDC74B as
            microtubule-associated proteins that localize to spindles and are
            important K-fiber crosslinkers required for bipolar spindle
            formation and chromosome alignment.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1290)
  AUTHORS   Woolfson,D.N.
  TITLE     The design of coiled-coil structures and assemblies
  JOURNAL   Adv Protein Chem 70, 79-112 (2005)
   PUBMED   15837514
  REMARK    Review article
REFERENCE   4  (bases 1 to 1290)
  AUTHORS   Burkhard,P., Stetefeld,J. and Strelkov,S.V.
  TITLE     Coiled coils: a highly versatile protein folding motif
  JOURNAL   Trends Cell Biol 11 (2), 82-88 (2001)
   PUBMED   11166216
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA375407.1, BC063387.1 and BC016861.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001258306.2.
            
            Transcript Variant: This variant (4) uses an alternate splice site
            in the coding region but maintains the reading frame, compared to
            variant 1. The encoded isoform (4) is shorter than isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063387.1, SRR1163655.294161.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409856.8/ ENSP00000387009.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 DA375407.1         55-63
            10-1289             BC063387.1         1-1280
            1290-1290           BC016861.1         1457-1457
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q21.1"
     gene            1..1290
                     /gene="CCDC74A"
                     /note="coiled-coil domain containing 74A"
                     /db_xref="GeneID:90557"
                     /db_xref="HGNC:HGNC:25197"
     exon            1..333
                     /gene="CCDC74A"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..1022
                     /gene="CCDC74A"
                     /note="isoform 4 is encoded by transcript variant 4;
                     coiled-coil domain-containing protein 74A; epididymis
                     secretory sperm binding protein"
                     /codon_start=1
                     /product="coiled-coil domain-containing protein 74A
                     isoform 4"
                     /protein_id="NP_001245235.1"
                     /db_xref="CCDS:CCDS58732.1"
                     /db_xref="GeneID:90557"
                     /db_xref="HGNC:HGNC:25197"
                     /translation="MSGAGVAAGTRPPSSPTPGSRRRRQRPSVGVQSLRPQSPQLRQS
                     DPQKRNLDLEKSLQFLQQQHSEMLAKLHEEIEHLKRENKDLHYKLIMNQTSQKKDSLS
                     MSSFQSVKSISNSGKARPQPGSFNKQDSKADVSQKADLEEEPLLHNSKLDKVPGVQGQ
                     ARKEKAEASNAGAACMGNSQHQGRQMGAGAHPPMILPLPLRKPTTLRQCEVLIRELWN
                     TNLLQTQELRHLKSLLEGSQRPQAAPEEASFPRDQEATHFPKVSTKSLSKKCLSPPVA
                     ERAILPALKQTPKNNFAERQKRLQAMQKRRLHRSVL"
     exon            334..378
                     /gene="CCDC74A"
                     /inference="alignment:Splign:2.1.0"
     exon            379..429
                     /gene="CCDC74A"
                     /inference="alignment:Splign:2.1.0"
     exon            430..568
                     /gene="CCDC74A"
                     /inference="alignment:Splign:2.1.0"
     exon            569..761
                     /gene="CCDC74A"
                     /inference="alignment:Splign:2.1.0"
     exon            762..835
                     /gene="CCDC74A"
                     /inference="alignment:Splign:2.1.0"
     exon            836..892
                     /gene="CCDC74A"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1290
                     /gene="CCDC74A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1269..1274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC74A"
                     /note="hexamer: AATAAA"
     polyA_site      1290
                     /gene="CCDC74A"
                     /note="major polyA site"
ORIGIN      
        1 atggtgacgg ggcgccaggc tagggcggcc tggccactga gccggggtgc agtggcagcg
       61 ggagagtacc tggcgatggc gatatgagcg gtgcgggggt ggcggctggg acgcggcccc
      121 ccagctcgcc gaccccgggc tctcggcgcc ggcgccagcg cccctctgtg ggcgtccagt
      181 ccttgaggcc gcagagcccg cagctcaggc agagcgaccc gcagaaacgg aacctggacc
      241 tggagaaaag cctgcagttc ctgcagcagc agcactcgga gatgctggcc aagctccatg
      301 aggagatcga gcatctgaag cgggaaaaca aggatctcca ttacaagctc ataatgaatc
      361 agacatcaca gaagaaagac agcctctcca tgtcaagctt ccagtctgtc aagtccatct
      421 ctaattcagg caaggccagg ccccagcccg gctccttcaa caagcaagat tcaaaagctg
      481 acgtctccca gaaggcggac ctggaagagg agcccctact tcacaacagc aagctggaca
      541 aagttcctgg ggtacaaggg caggccagaa aggagaaagc agaggcctct aatgcaggag
      601 ctgcctgtat ggggaacagc cagcaccagg gcaggcagat gggggcgggg gcacaccccc
      661 caatgatcct gccccttccc ctgcgaaagc ccaccacact taggcagtgc gaagtgctca
      721 tccgcgagct gtggaatacc aacctcctgc agacccaaga gctgcggcac ctcaagtccc
      781 tcctggaagg gagccagagg ccccaggcag ccccggagga agctagcttt cccagggacc
      841 aagaagccac gcatttcccc aaggtctcca ccaagagcct ctccaagaaa tgcctgagcc
      901 cacctgtggc ggagcgtgcc atcctgcccg cactgaagca gaccccgaag aacaactttg
      961 ccgagaggca gaagaggctg caggcaatgc agaaacggcg cctgcatcgc tcagtgcttt
     1021 gagccacccc aatctggtca gtgccaggcc caccaacctg cagctggaga ctggctctct
     1081 atagcatttc ctgatacttc cgctactttt aggcctggct aaattccaag acagataaca
     1141 ctcaagatag ataaagtact tgatctccaa actgacaaac tgtttatttt ctagctgtta
     1201 ttttgctatt tggcatttac ataaaagcac acgatgaagc aggtatcgcc ttacctgttg
     1261 aaactgaaaa taaagcttgt ttatttccaa
//
