LOCUS       NM_178335               8629 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens coiled-coil domain containing 50 (CCDC50), transcript
            variant 2, mRNA.
ACCESSION   NM_178335
VERSION     NM_178335.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Angioni,M.M., Floris,A., Cangemi,I., Congia,M., Chessa,E.,
            Naitza,M.R., Piga,M. and Cauli,A.
  TITLE     Molecular profiling of clinical remission in psoriatic arthritis
            reveals dysregulation of FOS and CCDC50 genes: a gene expression
            study
  JOURNAL   Front Immunol 14, 1274539 (2023)
   PUBMED   37965313
  REMARK    GeneRIF: Molecular profiling of clinical remission in psoriatic
            arthritis reveals dysregulation of FOS and CCDC50 genes: a gene
            expression study.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Jia,P., Tian,T., Li,Z., Wang,Y., Lin,Y., Zeng,W., Ye,Y., He,M.,
            Ni,X., Pan,J., Dong,X., Huang,J., Li,C.M., Guo,D. and Hou,P.
  TITLE     CCDC50 promotes tumor growth through regulation of lysosome
            homeostasis
  JOURNAL   EMBO Rep 24 (10), e56948 (2023)
   PUBMED   37672005
  REMARK    GeneRIF: CCDC50 promotes tumor growth through regulation of
            lysosome homeostasis.
REFERENCE   3  (bases 1 to 8629)
  AUTHORS   Hou,P., Lin,Y., Li,Z., Lu,R., Wang,Y., Tian,T., Jia,P., Zhang,X.,
            Cao,L., Zhou,Z., Li,C., Gu,J. and Guo,D.
  TITLE     Autophagy receptor CCDC50 tunes the STING-mediated interferon
            response in viral infections and autoimmune diseases
  JOURNAL   Cell Mol Immunol 18 (10), 2358-2371 (2021)
   PUBMED   34453126
  REMARK    GeneRIF: Autophagy receptor CCDC50 tunes the STING-mediated
            interferon response in viral infections and autoimmune diseases.
            Erratum:[Cell Mol Immunol. 2021 Dec;18(12):2682. doi:
            10.1038/s41423-021-00777-7.. PMID: 34654907]
REFERENCE   4  (bases 1 to 8629)
  AUTHORS   Hou,P., Yang,K., Jia,P., Liu,L., Lin,Y., Li,Z., Li,J., Chen,S.,
            Guo,S., Pan,J., Wu,J., Peng,H., Zeng,W., Li,C., Liu,Y. and Guo,D.
  TITLE     A novel selective autophagy receptor, CCDC50, delivers K63
            polyubiquitination-activated RIG-I/MDA5 for degradation during
            viral infection
  JOURNAL   Cell Res 31 (1), 62-79 (2021)
   PUBMED   32612200
  REMARK    GeneRIF: A novel selective autophagy receptor, CCDC50, delivers K63
            polyubiquitination-activated RIG-I/MDA5 for degradation during
            viral infection.
REFERENCE   5  (bases 1 to 8629)
  AUTHORS   Min,J.S., Halder,D., Yoon,J.Y., Jeon,S.J., Jun,S.Y., Lee,J.R.,
            Lee,J.J., Choi,M.H., Jung,C.R., Lee,D., Kim,B.J. and Kim,N.S.
  TITLE     Coiled-coil domain containing 50-V2 protein positively regulates
            neurite outgrowth
  JOURNAL   Sci Rep 10 (1), 21295 (2020)
   PUBMED   33277610
  REMARK    GeneRIF: Coiled-coil domain containing 50-V2 protein positively
            regulates neurite outgrowth.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8629)
  AUTHORS   Zhang,Y., Wolf-Yadlin,A., Ross,P.L., Pappin,D.J., Rush,J.,
            Lauffenburger,D.A. and White,F.M.
  TITLE     Time-resolved mass spectrometry of tyrosine phosphorylation sites
            in the epidermal growth factor receptor signaling network reveals
            dynamic modules
  JOURNAL   Mol Cell Proteomics 4 (9), 1240-1250 (2005)
   PUBMED   15951569
REFERENCE   7  (bases 1 to 8629)
  AUTHORS   Blagoev,B., Ong,S.E., Kratchmarova,I. and Mann,M.
  TITLE     Temporal analysis of phosphotyrosine-dependent signaling networks
            by quantitative proteomics
  JOURNAL   Nat Biotechnol 22 (9), 1139-1145 (2004)
   PUBMED   15314609
REFERENCE   8  (bases 1 to 8629)
  AUTHORS   Vazza,G., Picelli,S., Bozzato,A. and Mostacciuolo,M.L.
  TITLE     Identification and characterization of C3orf6, a new conserved
            human gene mapping to chromosome 3q28
  JOURNAL   Gene 314, 113-120 (2003)
   PUBMED   14527723
REFERENCE   9  (bases 1 to 8629)
  AUTHORS   Modamio-Hoybjor,S., Moreno-Pelayo,M.A., Mencia,A., del Castillo,I.,
            Chardenoux,S., Armenta,D., Lathrop,M., Petit,C. and Moreno,F.
  TITLE     A novel locus for autosomal dominant nonsyndromic hearing loss
            (DFNA44) maps to chromosome 3q28-29
  JOURNAL   Hum Genet 112 (1), 24-28 (2003)
   PUBMED   12483295
  REMARK    GeneRIF: identification of a novel DFNA locus on chromosome 3q28-29
            in a Spanish family with postlingual and progressive hearing
            loss[DFNA44]
REFERENCE   10 (bases 1 to 8629)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ557013.1 and AC073365.14.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_178335.2.
            
            Summary: This gene encodes a soluble, cytoplasmic,
            tyrosine-phosphorylated protein with multiple ubiquitin-interacting
            domains. Mutations in this gene cause nonsyndromic, postlingual,
            progressive sensorineural DFNA44 hearing loss. In mouse, the
            protein is expressed in the inner ear during development and
            postnatal maturation and associates with microtubule-based
            structures. This protein may also function as a negative regulator
            of NF-kB signaling and as an effector of epidermal growth factor
            (EGF)-mediated cell signaling. Alternative splicing results in
            multiple transcript variants encoding distinct isoforms. [provided
            by RefSeq, Oct 2008].
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the long isoform.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The extent of this transcript
            is supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.214301.1, AJ557013.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000392455.9/ ENSP00000376249.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2145              AJ557013.1         310-2454
            2146-8629           AC073365.14        10456-16939
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q28"
     gene            1..8629
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /note="coiled-coil domain containing 50"
                     /db_xref="GeneID:152137"
                     /db_xref="HGNC:HGNC:18111"
                     /db_xref="MIM:611051"
     exon            1..330
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     CDS             282..1730
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /note="long isoform is encoded by transcript variant 2;
                     coiled-coil domain-containing protein 50; protein Ymer"
                     /codon_start=1
                     /product="coiled-coil domain-containing protein 50 long
                     isoform"
                     /protein_id="NP_848018.1"
                     /db_xref="CCDS:CCDS33912.1"
                     /db_xref="GeneID:152137"
                     /db_xref="HGNC:HGNC:18111"
                     /db_xref="MIM:611051"
                     /translation="MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLAS
                     NVQRNRLVQHDLQVAKQLQEEDLKAQAQLQKRYKDLEQQDCEIAQEIQEKLAIEAERR
                     RIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAYADSYYYEDGDQPGSRRAREL
                     GSGFSRPCRLQRDGKTVKHKKEKPEHPLENLEEPEQHCSSKRSLSSSSSGKGRDNPHI
                     NNEQHERKRSTQERPRRPLLPTISGEVFLSTECDDWETKINHQTRNWEKQSRHQDRLS
                     PKSSQKAGLHCKEVVYGRDHGQGEHRKRRHRPRTPPFSESEEQLHLHDAGMKPRVMKE
                     AVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKA
                     YKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDEPHHSKNERPARPPPPIMTDG
                     EDADYTHFTNQQSSTRHFSKSESSHKGFHYKH"
     exon            331..393
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            394..520
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            521..611
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            612..729
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            730..1257
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1373
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1418
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1523
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1603
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1710
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..8629
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2114..2119
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /note="hexamer: AATAAA"
     polyA_site      2134
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /note="major polyA site"
     regulatory      4729..4734
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /note="hexamer: ATTAAA"
     polyA_site      4751
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
     regulatory      5532..5537
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /note="hexamer: AATAAA"
     polyA_site      5558
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
     regulatory      8377..8382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /note="hexamer: AATAAA"
     polyA_site      8398
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
     regulatory      8610..8615
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
                     /note="hexamer: AATAAA"
     polyA_site      8629
                     /gene="CCDC50"
                     /gene_synonym="C3orf6; DFNA44; YMER"
ORIGIN      
        1 atttccgggc tccggatatt tggtatcgat tggggccggg gacgcggagc aggtggccgc
       61 ggcggggcag ctgggccgcc agcttggtgc ctcggggacc gtctcccgct gctttggtca
      121 ccagcccctg cccgcccgac ccgctccgtt ctccggcctg cgagccctgc cggccggact
      181 ttgcgccgcg tccggcgctg ctgctgcgct cggggccccg ctcggcgccg gcggtgaccg
      241 ggaagcccgc gttaaagggg caaccgggac cctggcccgg tatggctgaa gtcagcatcg
      301 accagtccaa gctgcctgga gtcaaggaag tatgccgaga ttttgctgtc ctggaggacc
      361 acaccctggc tcacagcctg caggaacaag agattgagca tcatttggca tcgaacgttc
      421 agcggaaccg tttggtccag catgatctcc aggtggctaa gcagctccaa gaggaagatc
      481 tgaaagcgca ggcccagctc cagaagcgct acaaagacct tgaacaacaa gactgtgaaa
      541 ttgctcagga aattcaggag aagctggcta ttgaggcaga gagacgacgc attcaggaga
      601 agaaggatga ggacatagct cgccttttgc aagaaaagga gttacaggaa gagaaaaaga
      661 gaaagaaaca ctttccagag ttccctgcaa cccgtgctta tgcagatagt tactattatg
      721 aagatggaga ccaaccaggg tcaaggaggg ccagggaatt gggttctgga ttctcaagac
      781 cttgtagact ccaaagagat ggaaagactg tgaagcacaa gaaagagaaa ccagaacatc
      841 cactggagaa cttggaagag ccagaacaac attgttcatc gaagagatcc ctgtcatcct
      901 ctagctcggg caaagggagg gacaatcccc atattaacaa tgagcagcat gaaaggaaac
      961 ggtccactca ggagaggcct cggagacctc tgcttcccac gatcagtggt gaagtgtttc
     1021 tgagcactga atgtgatgac tgggagacta agattaacca tcagactcga aattgggaaa
     1081 aacagtctcg acaccaagat cgactttcac ccaagtcctc acaaaaagca gggcttcact
     1141 gcaaggaagt tgtatatggg agggaccatg ggcaaggtga gcacagaaaa aggagacaca
     1201 ggcccaggac tcctccattc tcagagagtg aggagcagct ccacctccat gacgcaggaa
     1261 tgaagccaag agtgatgaaa gaagctgtat ctactccatc acgaatggcc cacagggatc
     1321 aggaatggta tgatgctgaa attgccagaa aactgcaaga agaagaactt ttggctaccc
     1381 aggtggacat gagagccgct caagtagctc aagatgaaga aatcgctcga cttctaatgg
     1441 ctgaagaaaa gaaagcttac aaaaaagcca aggagcggga gaaatcatct ttggacaaaa
     1501 gaaagcaaga ccccgagtgg aagccaaaaa cagctaaagc agcaaattcc aagtcaaaag
     1561 agagtgatga acctcaccat tctaagaatg aaaggccagc acggccacca ccacctatca
     1621 tgacagatgg tgaagatgcg gattacactc attttacaaa ccagcagagt tccacacggc
     1681 atttctcaaa atcagagtcc tctcataaag gttttcatta caaacattaa aaacctagga
     1741 atctgccttg aaaatggact cactatagca aatattactg ggtgatacag aatgaattct
     1801 acacttactt tttttctcct gtgtttgcat tcctgggatt tatcctcaag tgcatttctg
     1861 accataagta attttaattc atttcaaatg ttttggttat tcatgatcac ttgggcagta
     1921 taagaaaatg tagcttctga atattggcca cctctatgct gcatatactt cttgggatat
     1981 agtatctaag acctttgtaa actgccattt tgttaggtat ggagtttggt atctagggag
     2041 taggccttat ttagcaattc aaattttatg gagatgaatg atcaaagtga aacaatgttt
     2101 ggatgcaacg cagaataaaa gaatataaga aatagctttt tgttgcatta atgtatgata
     2161 ttttgaagga cagagccttt gctttttgta ttctttaaga aaagcacaac cataaagtat
     2221 tttaaaaaaa gaaacacttg gagcaattga aatatgttat tctgactaga tggactgtag
     2281 aggttttgca tttctcagcc ctcctgggga gattggccta gatagcattc tttaacactt
     2341 cctctgcttt tgcctcagta gcaaggcttt cctaagctcc tgggcaatgg aacatttatt
     2401 agtatttatt gacaaaaatt tagttctctg aaattttaac tcatatatat gaaaaacttt
     2461 gtattccatc tcttcttttt catttacttg tagagccaag gaaatggaat tgaaagatga
     2521 ttgcataagt aatgatgcca tccttctctc tggctgtaga ctgaggcttt tctcttgctt
     2581 caagtcagag cagtatttgt tgataacctc tcaataatgt ttggtttaca tgccagtaat
     2641 taaattaatt caacatgaag ttgaatttga tgaagtggtc atctatccaa gtatttggtt
     2701 tttgttttgt tttgttttgt tttttgagtt ggagtctcgc cctgtcacac aggctggagt
     2761 gcagtggtgc aatcttggct cactgcaacc tccgccacct gggctggagc aattcccctg
     2821 cctcagcctc ccaagtagct ggaattacag gcatgtgtca ccacacccgg ctaatttttg
     2881 tatttttagt aaagagcggg tttcaccatg ttggccaggc tggtctcgaa cccctgacct
     2941 caagtgatcc acctgccttg gcctcccaag gtgctgggat tacaggctta agccaccatg
     3001 tccagccagc caagtatttg gttttaaaca tttgtattcc tttgtgcaag ggattgtatt
     3061 cctctgtaca agacctttaa gcacctcttc aagaaattta gtctttgtgt agctgctaac
     3121 cttactgcaa tatttatttt cccttatttt cccatactac ttagttgaca aatggcaatc
     3181 aagctcactt gttctccctc tctctgtctc ccccccatgt acacatatat atatcccttc
     3241 agcttgactt gctattattt ttcattgtat aaatatgaaa ttcttggcat ttcaatatgg
     3301 catttgttta gggaaaaaat atgtaggtca ctagagaaaa atgttaattt ttttcccact
     3361 gtgaattgcc ttctgtttgc cttatctttt cagatagtct ttaaagttgg gcaatattca
     3421 ggcagctatt tagaacagtt tctcagagtg gatttgggtc tctcatttga gaaagatttg
     3481 tagtttcaat gaagtttgta tattatagga atttaagtaa aatcatctga gttaagtttt
     3541 gggcaaatgt taatcagaaa gataatctac atccctactt tatgtagtca ttttggagaa
     3601 atggaggaaa aggaaaataa caggactttt tatagctgtg ataaaatggg taaagaaaaa
     3661 cctccattta aataaggaag gggagacaaa atggaaggtc ctggccttaa gtcaaattcc
     3721 agatagtgct ttcaatatcc aaagcttagg ccttgtttac ttatagtaat gctatatttt
     3781 tctttatctg ccctgtgatt tcgcttcgtg tccttcaaag tttttttatt gtaattttaa
     3841 aggaaaaaaa caatgtattt ttatttgata actaggcaaa atcatttgcc attttttatt
     3901 tcataaatat ttatttttct atctcataca atgatcagtt ttactttaaa aattctgcat
     3961 ttctcttgcc tctcatgtac ttcatccagt tatttattat tgtaaaatgt taactggaga
     4021 tactgcattc aaaaaaaagt cggaccactt aacacttacc aggaatgtta gtgtttcagc
     4081 tgttttagta agtgaccaag taagctatca ccttgtgata gctaattcaa catctttatt
     4141 gaaaatttgt tacttttttt aaaggttttt aaaagattac aattttaaaa tattttatgt
     4201 ttattttatt tttgtgtaaa gcaaacactt ttaaaataat atcagagtaa ttattaccct
     4261 atgaataagg agatagaaat gaaattcagc cagttaaatc ctcatactct ttctcattca
     4321 aataatataa taatgtttga gactatagaa gcaactcaac tgtattttaa tgaactgcca
     4381 gacacttttc actgtgttct ctgcttttta ctttgtcatt tttatataaa tgtgaaagca
     4441 ttttgttgtt gttgttgttg ttcctgctgc tagaacacat gcagtttaat ttttaggccc
     4501 acagtgtatt atgctttaat gttgtgatgt aaactaatac ttctggcctg ggaaaacctt
     4561 tctgtttctg tatctttgtc ctttggaggc caggcctcat taacaccagt attgttgaat
     4621 aagcacaata tatgacggag ccttatcata aggctgcttg acatagacac ccactctcct
     4681 ccacatttta taaatcattg tttggaagta aagttagata agagtcaaat taaatgtttt
     4741 gacttgaaaa atatttgact tttgcgttca aaccactgtt agacgtaagt attttattag
     4801 acataagtat tttattttgg gactagacag ttgctttaca tgtatttaag ggaacattgt
     4861 gattttcaga aagtggcttt acatactatt gaattgctat ttcccaagtt actttacaac
     4921 cagcagcagc ataccaataa attacaaaag cagaaaatga aaattaaccc taggtgttct
     4981 gatgatagag aaatagctaa attagaaaag aaagatatgg atagatgctt ttaggaggaa
     5041 aaccttttta gaatggcaat gaccacttgg atctaggtca acatattcca aaatgtgtga
     5101 ccttgggctt tgtgtttact gaactcactc tagaaaattc tggacctgat tcattaaccc
     5161 cgcttttctt ctctaatgtg tcctgaagct gagctagatg atgagtaaat tctttgctga
     5221 ctgttgctca tcactttctc tcaaagttag aacttttcag tataaaaata attagctttt
     5281 aactgattat taattttctt taatagtttc tgtcaaaact tgtctaaaat ttgtgttgtg
     5341 ccaaattgga aatacccact ataatatggt gcattcagtt tactccacaa gtggttaaca
     5401 ttttaagtcc tagatttttt atgtcttgcc tttgtataag catcgctttg gggaatacca
     5461 agatgattca ttattcagtg attattttct tgaaagataa ctatgatgat tggctgtaaa
     5521 atcttcaccc taataaagga gtgagatctc tgtgtaaaca gctttctgaa ggactgataa
     5581 tagaatagaa cttgcagcca aaggcttttt cccttttgag aagacggtat ttgtgtaagg
     5641 agtgctaagg tagacttcat tgtacataga gtgccatatt ctgttgaaac gatcataaca
     5701 aaatcaaaac ctcatgcttc agcaaaaagg aaaaaagatt tttaaattac tttgtctatt
     5761 acttactgct catagagaag tgactatata aaatggtcca attttctcat ttctggtggt
     5821 gcctgtgagt cttaaagcca tatctgctta tcttactgtt gatagaatac tttttttctt
     5881 tttagcccat gataagtaat atgggcaggt agaagcaaac tggagactgt cctgttaatg
     5941 ctgcatgggg cacaatgtct ttcaaattat tggtggaaga atctctttta tccatggcga
     6001 gaggtaagaa tattaaagag tcaggatatt gttgctcctg gtatttaaca actgtcatta
     6061 gaaatgctga aatttaattg tagagaagtc aagaaagaaa gagtaaactg agaaaatctt
     6121 actactacag agtcactttt acaatgagac ctgcacttta tgccagcact gtttgtgagg
     6181 agcagtactg ccttaaatta gtttgactct gactgatgtc agtgtacgtt acttccttta
     6241 tgtgactgac agtcagcttt ccattgagat ataacattca cagatgatcc ccaagtatcc
     6301 ttgtcatcag aagctttgga ccttttctgc cactgacttg tggctgatct gtaacacaat
     6361 aaatacataa ttttatttca tgttgtctgt ccacctctga ataagcagtg gcatgaccaa
     6421 aaagactggt agaaggagag aatttcttgg ccaaatcctt cataccaggt accacttctt
     6481 gagtaactga gagttgactg tcttctatgg tgactacata gaagatattt ccaaaggttc
     6541 tgttatgcag tgaagacatt cagattattt gtaagaattt caccaacacc cttgtgtatt
     6601 gaaatgctta accattaaca gggaaaaaaa aggagaaaac cgaacaaaac agagacattt
     6661 acttgaaatg ggaggtttgc taatgtgaag agtgtttctg ttttattact tataagagaa
     6721 aagcaggaaa acttttgctt tgataagaaa gacaccagag tggtgattct gtttgatgcc
     6781 acaggctaac acaatcgtgt aatcatgttg agttgtagaa atatatggcc ctaaaaacca
     6841 ttctctttcc cttatatata taaatatgtg aacactgcct agtaacattt taatagattt
     6901 aagatgcatt tgtacattct cagcaaacta gaagaaaatg ttttttctct ttttgagtta
     6961 acttcatagc atagagaacc agcactgtgc aggtttcagc atttcagata agggcataat
     7021 taattgtgta acttaatgcc ctgttattca ttgctgaggt tcatataaag aatagaaaaa
     7081 ttgaaagatg ggtttgggcg acattttctt cccaaaagat caagtaagga gtaagaacaa
     7141 gtaagggaag acagctaaga gaacatacta tgacatatca tagaattggg atacattttt
     7201 gttgcactgg atttcaggct atactgctaa aataatttct acctgttaaa ttttgtgaga
     7261 gggtttttga catcagaatt tttgtatttc tggtcaaatt ggtcttggaa atccctatgg
     7321 tctctaattt cagctcttgc tgtaaactgc aagccaaatg ctctttctca gaaagagcta
     7381 tatttttctc ttcttacagg caacccaata tttatttcat aaagtgttat aaatattgag
     7441 aagatacact ggggagatag catataaaaa tgatgctcca aaatgagaat tctatatggg
     7501 gctttcccta agaagaatcc atcccagcac atattttgaa aaggtgctga attaaaaagt
     7561 acaagagttt gctcatataa atcaagttgc ccaggaaacc tgaggtgatg ctatcatttg
     7621 tctcataatt tagaaacgtg atctcttgag agagagacta cgcattgcct gggcactttc
     7681 agattcctta ggaagtgccc ctttgatatt tggaatgtgg atatatttat aaaacaaatg
     7741 gattgttatt ccaaatccac atggatttat aaccaagccc cagaagaata ggcagctttg
     7801 aaatagctag ttcgtggaat tgaacagaac cttgatggaa tgcagttgcc tgtgtgagga
     7861 cagaaaacaa agaggctgcc tcagaccaca tgcctatcta gagattaagt gaatttgtga
     7921 gggacatttt gtggatgcct taaagatgac cagtggtggg ttctgatggg aatatataca
     7981 ctcatctgga ctaggccaat ggaagcagtc tctttcagtt cacctccgta cagcacaagt
     8041 tgttcctgct catgacctca ttgaggaaaa tgagaattgt ggtgctggtg actttcatgt
     8101 gtcttgggag gttgaggtgt tcaacatcgt taaggcactc ccaaaacgca aacctccttt
     8161 tttaaatgcc aattgtacat ctacattaat tcatctatgc aaacttgtgt tttcatggtt
     8221 tgttttttac actatatttc tcattaggtt ctttaaacat caggtttaat attgatatta
     8281 tgaataattt ttaaaccaaa gtattctata agtctgtgtg ctttgttttc ctggatggtt
     8341 tgaccaaggt aaacatcagt cttgtccttc tctcttaata aagtcatcca tttgttaagt
     8401 cagtggtctc tgtctcatgt ttttgttttg tttctttaca gcattcaagg acagggaaac
     8461 ttacgagagt cttattataa ttattattat ttacaaaatg accctaatag gtctgtttag
     8521 taacagtgaa attttttctt actccatttc catacacttt ctgtaaatat gtataacatg
     8581 ataatgatgc ctggtgtatt gaaatcctca ataaatactg ccaagtgtg
//
