LOCUS       NM_173648               9205 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens coiled-coil domain containing 141 (CCDC141),
            transcript variant 1, mRNA.
ACCESSION   NM_173648 XM_351507
VERSION     NM_173648.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Hou,Q., Wu,J., Zhao,Y., Wang,X., Jiang,F., Chen,D.N., Zheng,R.,
            Men,M. and Li,J.D.
  TITLE     Genotypic and phenotypic spectrum of CCDC141 variants in a Chinese
            cohort with congenital hypogonadotropic hypogonadism
  JOURNAL   Eur J Endocrinol 183 (3), 245-254 (2020)
   PUBMED   32520725
  REMARK    GeneRIF: Genotypic and phenotypic spectrum of CCDC141 variants in a
            Chinese cohort with congenital hypogonadotropic hypogonadism.
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 9205)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 9205)
  AUTHORS   Turan,I., Hutchins,B.I., Hacihamdioglu,B., Kotan,L.D., Gurbuz,F.,
            Ulubay,A., Mengen,E., Yuksel,B., Wray,S. and Topaloglu,A.K.
  TITLE     CCDC141 Mutations in Idiopathic Hypogonadotropic Hypogonadism
  JOURNAL   J Clin Endocrinol Metab 102 (6), 1816-1825 (2017)
   PUBMED   28324054
  REMARK    GeneRIF: These studies confirm that inactivating CCDC141 variants
            cause normosmic idiopathic hypogonadotropic hypogonadism but not
            Kallmann syndrome .
REFERENCE   5  (bases 1 to 9205)
  AUTHORS   Hutchins,B.I., Kotan,L.D., Taylor-Burds,C., Ozkan,Y., Cheng,P.J.,
            Gurbuz,F., Tiong,J.D., Mengen,E., Yuksel,B., Topaloglu,A.K. and
            Wray,S.
  TITLE     CCDC141 Mutation Identified in Anosmic Hypogonadotropic
            Hypogonadism (Kallmann Syndrome) Alters GnRH Neuronal Migration
  JOURNAL   Endocrinology 157 (5), 1956-1966 (2016)
   PUBMED   27014940
  REMARK    GeneRIF: report of a CCDC141 mutation as a new example of genetic
            aberration associated with Kallmann Syndrome
REFERENCE   6  (bases 1 to 9205)
  AUTHORS   He,J., Kelly,T.N., Zhao,Q., Li,H., Huang,J., Wang,L., Jaquish,C.E.,
            Sung,Y.J., Shimmin,L.C., Lu,F., Mu,J., Hu,D., Ji,X., Shen,C.,
            Guo,D., Ma,J., Wang,R., Shen,J., Li,S., Chen,J., Mei,H., Chen,C.S.,
            Chen,S., Chen,J., Li,J., Cao,J., Lu,X., Wu,X., Rice,T.K., Gu,C.C.,
            Schwander,K., Hamm,L.L., Liu,D., Rao,D.C., Hixson,J.E. and Gu,D.
  TITLE     Genome-wide association study identifies 8 novel loci associated
            with blood pressure responses to interventions in Han Chinese
  JOURNAL   Circ Cardiovasc Genet 6 (6), 598-607 (2013)
   PUBMED   24165912
REFERENCE   7  (bases 1 to 9205)
  AUTHORS   Fukuda,T., Sugita,S., Inatome,R. and Yanagi,S.
  TITLE     CAMDI, a novel disrupted in schizophrenia 1 (DISC1)-binding
            protein, is required for radial migration
  JOURNAL   J Biol Chem 285 (52), 40554-40561 (2010)
   PUBMED   20956536
  REMARK    GeneRIF: CAMDI is required for radial migration probably through
            DISC1 and myosin II-mediated centrosome positioning during neuronal
            development.
REFERENCE   8  (bases 1 to 9205)
  AUTHORS   Ehret,G.B., O'Connor,A.A., Weder,A., Cooper,R.S. and Chakravarti,A.
  TITLE     Follow-up of a major linkage peak on chromosome 1 reveals
            suggestive QTLs associated with essential hypertension: GenNet
            study
  JOURNAL   Eur J Hum Genet 17 (12), 1650-1657 (2009)
   PUBMED   19536175
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 9205)
  AUTHORS   Morris,J.A., Kandpal,G., Ma,L. and Austin,C.P.
  TITLE     DISC1 (Disrupted-In-Schizophrenia 1) is a centrosome-associated
            protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL:
            regulation and loss of interaction with mutation
  JOURNAL   Hum Mol Genet 12 (13), 1591-1608 (2003)
   PUBMED   12812986
REFERENCE   10 (bases 1 to 9205)
  AUTHORS   Balasubramanian,R. and Crowley,W.F. Jr.
  TITLE     Isolated Gonadotropin-Releasing Hormone (GnRH) Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301509
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092640.3, AL832777.1, AC093792.2, BC115378.2, AC023270.7 and
            AK096821.1.
            
            On Jun 2, 2019 this sequence version replaced NM_173648.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The transcript structure is
            predicted based on partial transcript alignments; it is unclear if
            this exon combination exists in vivo.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.2120041.1,
                                           SRR18074969.2036892.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000443758.7/ ENSP00000390190.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                AC092640.3         118508-118585       c
            79-672              AL832777.1         1-594
            673-673             AC092640.3         43554-43554         c
            674-1187            AL832777.1         596-1109
            1188-1188           AC093792.2         56805-56805         c
            1189-1873           AL832777.1         1111-1795
            1874-3873           BC115378.2         1-2000
            3874-3874           AC023270.7         96026-96026         c
            3875-5287           AK096821.1         331-1743
            5288-9205           AC023270.7         88242-92159         c
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.2"
     gene            1..9205
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="coiled-coil domain containing 141"
                     /db_xref="GeneID:285025"
                     /db_xref="HGNC:HGNC:26821"
                     /db_xref="MIM:616031"
     exon            1..298
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..4789
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="isoform 1 is encoded by transcript variant 1;
                     coiled-coil protein associated with myosin II and DISC1;
                     coiled-coil domain-containing protein 141"
                     /codon_start=1
                     /product="coiled-coil domain-containing protein 141
                     isoform 1"
                     /protein_id="NP_775919.3"
                     /db_xref="GeneID:285025"
                     /db_xref="HGNC:HGNC:26821"
                     /db_xref="MIM:616031"
                     /translation="MSSQGSPSVALSTTTVSSVAVQAGDSKIVIAVIKCGKWVQLQLA
                     ESQPNLLEIGSSQDETKKLLHDHELLLAKLKALEDRVWELLQEADKTAEENKDQSQVY
                     DAMAETLGEAWAALVSMLERRTELLRLTSEFFENALEFAIKIDQAEDFLQNTHEFESA
                     ESLKSLLQLHEHHTKELLERSLALLNKSQQLTDFIEKFKCEGPNVNPELTQGAHSSCL
                     KVDRLLELLQDRRRQLDKYLKQQWQELSQVLQICQWDQQENQVTCWFQKTIRNLQEQS
                     LGSSLSDNEDRIHKQEELIIKAKEWNSAVEKLKSEALRILLSKDYVEKEHLQLSHQKL
                     SQLQEEFGQLMVERNTWLKKANEFFNSANKAFDVLGRVEAYLKLLKSEGLSLAVLAVR
                     HEELHRKIKDCTTDALQKGQTLISQVDSCSSQVSGIHEMMGCIKRRVDHLTEQCSAHK
                     EYALKKQQLTASVEGYLRKVEMSIQKISPVLSNAMDVGSTRSESEKILNKYLELDIQA
                     KETSHELEAAAKTMMEKNEFVSDEMVSLSSKARWLAEELNLFGQSIDYRSQVLQTYVA
                     FLKSSEEVEMQFQSLKEFYETEIPQKEQDDAKAKHCSDSAEKQWQLFLKKSFITQDLG
                     LEFLNLINMAKENEILDVKNEVYLMKNTMENQKAEREELSLLRLAWQLKATESKPGKQ
                     QWAAFKEQLKKTSHNLKLLQEALMPVSALDLGGSLQFILDLRQKWNDMKPQFQQLNDE
                     VQYIMKESEELTGRGAPVKEKSQQLKDLIHFHQKQKERIQDYEDILYKVVQFHQVKEE
                     LGRLIKSRELEFVEQPKELGDAHDVQIHLRCSQEKQARVDHLHRLALSLGVDIISSVQ
                     RPHCSNVSAKNLQQQLELLEEDSMKWRAKAEEYGRTLSRSVEYCAMRDEINELKDSFK
                     DIKKKFNNLKFNYTKKNEKSRNLKALKYQIQQVDMYAEKMQALKRKMEKVSNKTSDSF
                     LNYPSDKVNVLLEVMKDLQKHVDDFDKVVTDYKKNLDLTEHFQEVIEECHFWYEDASA
                     TVVRVGKYSTECKTKEAVKILHQQFNKFIAPSVPQQEERIQEATDLAQHLYGLEEGQK
                     YIEKIVTKHKEVLESVTELCESLTELEEKLKQGDVLKMNPNLEDFHYDYIDLLKEPAK
                     NKQTIFNEERNKGQVQVADLLGINGTGEERLPQDLKVSTDKEGGVQDLLLPEDMLSGE
                     EYECVSPDDISLPPLPGSPESPLAPSDMEVEEPVSSSLSLHISSYGVQAGTSSPGDAQ
                     ESVLPPPVAFADACNDKRETFSSHFERPYLQFKAEPPLTSRGFVEKSTALHRISAEHP
                     ESMMSEVHERALQQHPQAQGGLLETREKMHADNNFTKTQDRLHASSDAFSGLRFQSGT
                     SRGYQRQMVPREEIKSTSAKSSVVSLADQAPNFSRLLSNVTVMEGSPVTLEVEVTGFP
                     EPTLTWYKKGQKLSADGHLQVLHKETRHSVFIPKVCKADAGLYVARAQNSSGALSSNV
                     ILHVTGNCRLPITRVNWITLCVVYVSVSLMYWLLTQ"
     exon            299..421
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            422..613
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            614..722
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            723..976
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1093
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1288
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1461
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1603
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1723
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1915
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2095
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2275
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2401
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..2590
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..2770
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            2771..2920
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            2921..3061
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            3062..3256
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            3257..3440
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            3441..3553
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            3554..3670
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            3671..4521
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     exon            4522..9205
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6274..6279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="hexamer: ATTAAA"
     polyA_site      6295
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="major polyA site"
     regulatory      6321..6326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="hexamer: AGTAAA"
     polyA_site      6339
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="major polyA site"
     regulatory      6366..6371
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="hexamer: ATTAAA"
     polyA_site      6384
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="major polyA site"
     regulatory      6413..6418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="hexamer: ATTAAA"
     polyA_site      6435
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
     regulatory      7711..7716
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
                     /note="hexamer: AATAAA"
     polyA_site      7730
                     /gene="CCDC141"
                     /gene_synonym="CAMDI"
ORIGIN      
        1 gctcctttct ctctcacgat aaactcaatg gggttgtcta tttttaacct cctgttcttt
       61 ccttttggca gagtaatcag tgtgagctga gctctttccc tgaatgaatc caagtaaccc
      121 tggccttctg aaatgaccag cagacacaca actgtccaga ggctgcccaa agtataaact
      181 ctggttctaa agtaccatgt ccagccaagg aagtcctagt gttgcgcttt ctacgacgac
      241 agtcagttca gttgctgtgc aggctgggga ctccaaaatc gttatagctg tcataaagtg
      301 tggcaaatgg gtacaacttc aactggctga atcacagccc aatcttctag aaattggcag
      361 cagtcaagat gaaaccaaaa aacttcttca tgatcatgaa cttcttttgg ccaagctcaa
      421 ggctttggaa gatcgggtat gggaactctt gcaggaagca gacaagacag ctgaagagaa
      481 caaggatcag agtcaggtct atgatgccat ggccgagact ctgggtgaag catgggcagc
      541 tctggtgtcc atgcttgaaa gaagaacaga gctccttagg ttgacttctg aattttttga
      601 aaatgcctta gagtttgcta ttaaaataga ccaagctgaa gatttcctcc agaatactca
      661 tgagtttgag agtgctgagt ccttaaaatc acttcttcag cttcatgaac atcatactaa
      721 agaactcttg gaacggtctt tagccctttt aaacaaaagt caacaactca ctgacttcat
      781 agaaaaattc aagtgtgaag gacctaatgt gaatcctgag ttgactcagg gagctcatag
      841 cagctgtctg aaggttgacc gccttcttga acttctacaa gacaggagaa gacaactaga
      901 caagtacttg aagcaacagt ggcaagaatt gagtcaagtt ctgcagatat gtcagtggga
      961 ccaacaagaa aaccaggtta cttgttggtt tcagaaaact ataagaaatt tacaggaaca
     1021 aagtctaggt tcatcacttt cagacaatga ggatcgaatt cataagcaag aggaactgat
     1081 aataaaagca aaggaatgga attctgctgt tgagaagctg aagagtgagg cactgagaat
     1141 tctgctgtca aaggactacg tggagaaaga acacctccag ctctctcacc agaaactcag
     1201 tcagcttcaa gaagaatttg gtcaactcat ggtggaaagg aatacctggt taaagaaggc
     1261 gaatgaattt tttaacagtg ctaacaaggc atttgatgta cttggaagag ttgaagctta
     1321 ccttaagctc cttaaatcag agggtttaag tctggctgtt ttggcagtga ggcatgagga
     1381 attacacaga aaaattaaag actgcacaac tgatgctttg caaaagggac aaaccttaat
     1441 cagccaagta gactcctgca gctctcaggt gtccggcatc catgagatga tggggtgcat
     1501 taagagacga gtggatcatc tgaccgaaca gtgttcagcg cacaaggaat atgctcttaa
     1561 gaaacaacaa ctaacagcct cagtggaggg ttacctacgg aaggtggaaa tgtcaattca
     1621 gaaaatcagt ccagtacttt ctaatgcaat ggatgttggt tctacccgtt ctgaatcaga
     1681 gaagattttg aataaatatc tggaactaga tatccaagct aaggagacat cacatgaatt
     1741 agaagcagct gcaaaaacca tgatggagaa aaatgaattt gtatctgatg aaatggtatc
     1801 actttcctct aaagctagat ggctagcaga agaattaaac ctatttggcc aaagcattga
     1861 ctatagatcg caagtcctgc aaacttacgt ggcatttctg aagtcatcag aggaggtaga
     1921 gatgcagttt cagagcttaa aagaatttta tgaaaccgaa atccctcaga aggagcagga
     1981 tgatgctaaa gccaagcatt gttctgactc ggctgagaag cagtggcagc tatttttaaa
     2041 gaagagtttt ataacacaag atctagggct tgagttcctt aatttaataa atatggcaaa
     2101 agagaacgag atattagatg tgaaaaatga agtgtacctc atgaagaaca ccatggaaaa
     2161 ccagaaagca gaacgggaag aacttagcct ccttcggctg gcatggcagc ttaaagccac
     2221 ggaaagcaag cctggaaaac agcagtgggc ggcattcaaa gagcaactta aaaagacttc
     2281 tcacaactta aaacttcttc aggaagcact tatgcctgtg tctgcacttg acctcggagg
     2341 gagcctccag ttcattttag atctacgaca aaaatggaat gacatgaagc ctcagttcca
     2401 gcaattgaat gatgaggttc agtacattat gaaagaatca gaggagttaa ctggcagagg
     2461 agcccctgta aaagaaaagt ctcaacaact gaaggacctt attcacttcc atcaaaaaca
     2521 gaaagagaga atccaggatt acgaggatat cctgtacaag gtggtccagt tccatcaagt
     2581 caaggaagag ctgggacgtc tcatcaaatc aagagagctg gagtttgtag agcagccgaa
     2641 ggaactgggt gatgcccatg atgtgcagat tcacctccgg tgctctcagg aaaagcaagc
     2701 ccgtgtagac catctccaca gactggccct ttccttagga gtcgacatca tctcatcagt
     2761 gcagcggcct cactgctcta atgtttctgc aaagaaccta cagcagcagc tggagctcct
     2821 tgaggaggac agcatgaagt ggcgtgccaa agctgaggag tatggacgga ccctgtcccg
     2881 tagtgtggag tactgcgcca tgagagacga gataaatgag ctcaaagact cattcaaaga
     2941 tatcaaaaag aaattcaata atttgaagtt taattacact aagaaaaatg aaaaatctcg
     3001 gaatctgaag gcgcttaaat atcaaattca gcaagttgat atgtatgctg aaaaaatgca
     3061 ggctttgaaa aggaaaatgg aaaaagttag taataaaacc tctgattctt tcttaaatta
     3121 tccaagtgat aaagttaatg tccttttgga agtcatgaag gatttgcaaa aacatgtgga
     3181 tgactttgac aaagttgtga cagattacaa gaagaatttg gacctgactg agcatttcca
     3241 ggaggtgata gaagagtgtc atttttggta cgaagatgca agtgccacag ttgtaagagt
     3301 tggaaaatat tccacagagt gcaagacaaa ggaagctgtg aaaattctcc accagcagtt
     3361 taataagttt attgcaccct cagtgccgca gcaagaagaa aggattcagg aggccactga
     3421 ccttgctcag cacttatatg gtttggaaga aggacagaaa tatattgaga aaatagtgac
     3481 aaaacacaaa gaggttcttg aatctgtgac tgaattatgt gagtccctca cagagctcga
     3541 agaaaaactg aagcagggag atgttttaaa gatgaatccg aatttggaag acttccatta
     3601 tgattacatt gacttgctaa aggaaccagc aaaaaataag cagacaatat tcaatgaaga
     3661 aaggaataag gggcaggtgc aggtggcaga tcttttgggc atcaatggaa caggggaaga
     3721 gcgactacca caagacctga aggtgtccac tgacaaggag ggtggcgtcc aggacctgct
     3781 cctgcctgaa gacatgctct caggggaaga atatgagtgt gtctcacctg atgacatctc
     3841 cttgcctcct ctcccaggaa gccctgagtc ccctcttgca ccatctgaca tggaggtgga
     3901 agagcctgtc agctcctccc tcagccttca cataagcagc tatggggtgc aggctgggac
     3961 cagcagccca ggggatgccc aggaatctgt tcttccacca cctgttgcct ttgcggatgc
     4021 atgcaatgat aagagagaaa cattttcaag tcattttgag aggccttacc tccagttcaa
     4081 agctgagccc ccactaacct ccagaggatt cgtggaaaag agtactgcct tacacagaat
     4141 cagtgctgaa catccagaga gcatgatgag tgaagtgcat gagagagctt tacagcagca
     4201 ccctcaggct cagggtggtt tgctagaaac acgggagaaa atgcatgctg ataataactt
     4261 cactaaaacc caagataggc tgcatgcttc ctctgatgca ttctcgggcc tcaggtttca
     4321 atcaggcacc agcaggggct atcagaggca aatggttcct cgagaagaga ttaaaagcac
     4381 atcagcaaag agcagcgtgg tcagcctagc tgaccaggca cctaatttct ccaggctcct
     4441 gtctaatgta actgtcatgg aaggttctcc agtgactttg gaagttgaag taacaggatt
     4501 tccagagcct acactgacat ggtacaagaa gggccagaaa ttgtctgcag atgggcactt
     4561 acaggtttta cacaaggaga caaggcattc ggtgttcatt ccaaaggtat gcaaggcaga
     4621 cgcaggcctc tatgtggctc gggcccaaaa ctctagcggc gctctctctt ccaatgtcat
     4681 cctccacgtg acaggtaact gcaggctgcc aatcacaaga gtaaactgga taaccctgtg
     4741 tgtcgtctat gttagtgtgt ccctaatgta ctggctcctc acacaataac tgtggtgttg
     4801 gcaccaatgg acatcattct catgtgccta aagaaaagtg ccgccgttta tcttcctgca
     4861 cctcctgcaa agcatctccc accaagtgta tctccagtct gatcaccata cttgtttggc
     4921 taataccact gctaggtgaa ataatcaaat tttctggagc gtttaatata tttgagaagc
     4981 ttggtaaatt atctgtagtg gataagaaca gatttttgta ctctagaaac agatgttttc
     5041 aacataatat gcagcaaaat tattctatgc ctattccctc ctaatttaag ggcgtgcaga
     5101 tgaaggctga catcggcata gaaatgtgct aaatttcaaa tatggttgtt caataatata
     5161 tttttaattg tattttaaaa catccctttg tagggaatat cagtagaact attgaattga
     5221 tgttgtattt ttgcattagc tgtcctttaa ttttgctttc catgtcttga tcttgttacc
     5281 gaaatgcatt ctgtgtaatt tcaacgtaga ctacgttcct gagtctgatg atgtcattgt
     5341 aaataatcat tcctcatgtt gctctcacgg gtttgacatt ggaggataga tgtgccatca
     5401 actttaaata gagtttccat cttttgtcag ctctgcatgt catacagcaa gatggaacac
     5461 aggttgcaag aaagaaccaa cgttattcct gacattctat tgatgtgttt tgagcagcaa
     5521 tgtgtaacca ggtgtttctg cttctaatct tgattctgct gatgaccatt tttatacttt
     5581 atggcaaagc ctcattgcct cagtttcctg tctggaaaat gagttcatca tctacctctt
     5641 gggcaattga gagttttcat tttgtgtctt ttaaacatct ggaaaatgga aaacactgag
     5701 ggctaaggat aatgatattg tttaatgtaa tttgtcatca gaccggcctc ctgctcacta
     5761 tttctttttt tttagatgct gtttttttag aatattaaat taaaggagtg ctgttaatat
     5821 gtaatcttca actagttcca tggaggaaag tgatgtttta tgaattagag cttaaattcc
     5881 tatctaattt ctgaagactg cattcttttc aaagacttga aattaaactc aggaggatac
     5941 attttgtttg ttctaggggg aaacgtaata gaatggtatg agatgaattc tatcctttta
     6001 caataattat tactataata ttgaagtatt ctatgccttt ctgaaacact ttattcagaa
     6061 aagatcattt tttggcttcc ttttaaaaat aagcttgaag tagggaagca tttgcttctt
     6121 agattttaaa tgtagtctta ttatgctgag gaaaatcttt agttgaaaga ataattcatt
     6181 acagattttt gatgaaggga agaaattaat tatgttgctg gaagactgtc tacaaataat
     6241 caaaatattt aaatgtaatg tattcaagtt gttattaaaa agttactgaa tgttatctga
     6301 agatgtgaaa tgtgtagtca agtaaaaagt tggcgtatac tttcttgggc tgcatatcca
     6361 atttaattaa aaaattaaat ctcagtttcc ttctaaattt tgatagaact atattaaaag
     6421 cttgtataca cagcattcta ggaaatttct cttaactatc ttttttttgt tttttttgtt
     6481 tttttttttt ttttttgaga aagagtcttg ctctcttgcc cagtgcagtg gtgccatctt
     6541 ggctcactgc aacctccacc tccgggttca agcaattctc ctgcttcagc ctcctgagta
     6601 gctaggatta caggcatgcc tacctaatat ttacattttt agtagaaacc aggtttcacc
     6661 atgttggcca ggctgggcct cagcctccca aagtgccagg attacaggta tgagccacca
     6721 tagccggcct cttgtatcct ttttgtccaa tttcaaataa tgtgtgaatc ttgcctaact
     6781 gctgttgccc acgttgaagc taaagagtaa aaacctattc atgaacaaag aaggcaggtg
     6841 agacccggta acaaactgtc tctcttccca ctttctcttt cttattaatt tggcaggatc
     6901 tcccttccca ttttgtcaac atggttgaat ttgtgtgttg gggttgttag ggcatgggaa
     6961 ttcctggttt gctttctcta ttgcatgctt gttcatgtta actctttggg gagcagaatg
     7021 agatacatat gcatccatgg ttatgatgga aaaaagcatg caaaatccat ttttagagag
     7081 agagagaaag agaaaggtgg gggtggggga gcacaaagat cagaagagta ctatgctgat
     7141 attcaagata tagtacagat agctttctgt gtacctatcc atctacctat tcatctgggc
     7201 tattgattga ctcccacagc agtaaagaat aattacgcag ttattcttct ggttatgttt
     7261 taatttcaaa aagttaagtg tgattttcct ttttgctggg atttctgtct tgagcaaata
     7321 attattctta tgaaagtatc aattgcagtt actggttaaa aatgtaagac ctaggaaatt
     7381 taagtgttgt ttctatttta aggtatatac ataatttata accatttact tctagagaaa
     7441 atcctgaatg gttagtaata tcaaaacatt ttcaacaaga aaactaaaat gaaaagaaag
     7501 tttaaaaatt acatgtgtta cagctctttt agaatttgtc tagcaggttt tctggtgaaa
     7561 attaaactca agattttcac cagaaaacgt tttaaaactt ctaaactctt aaacaaatta
     7621 taaatacttg atactttgaa gaactgcaaa acaaaatgtt tttaagtttt cataaggcaa
     7681 aatcattgag ttttccagct ttgattttcc aataaaagga tgtataaata tttggtggta
     7741 gtttttcaca ctcaattcca agagaaattt actgtaggaa tctgtacatt tgaatctctg
     7801 aatattctat gatatttaaa atatttgaac aagatgcaga aaatttttta gctatattaa
     7861 tcaataatgg taacctctat cactcataat caagacacat acttaaaata gatgactaca
     7921 agacccttct aactgctttg ctcaaattta aatttcctca agtagttcat agacccttta
     7981 caacctggcc tcccctctct ggcctcggtt tgatcccctc ctattacccc caacccctag
     8041 taaagtgaca tttaacttca gggttttaac atatcatgct cttctctcca gccctttctt
     8101 cctataactc tcttactatc ttcaccaccc tatcttcatg tgacttaact cttgcttatg
     8161 ttccagttct gctgagacac aacttcccct gggaagcctc ctgacccatc aagtccagag
     8221 tagatggtcc tgccatgtgc actgtcagca cctgcactgc ccagccacgc ccctgaatat
     8281 acacaaagga atcgcaggtt tgagcatcag tctcctctgg ctaggctgta agaaccttat
     8341 cagcaggatt catgacagcc ctgttgtcat tgtattgcca gtacctaaga cacagtcaca
     8401 ctcagtaaac tcttttgaat tgaattgttg ccaaaggtaa acaaagataa acctagcttg
     8461 tttttctccc ctttttagag ggaggagagg ggaaatgtaa aaagacaaag atgattctcc
     8521 cttcttatag acaggagtca atccgaatca ggccctaaca atgaagcact catgaaaatc
     8581 tctcacctca tctgcaaaac ttgtgtgaga accctccaga ccctctgggc tagaccctga
     8641 ttgaaaattt gaatttaaaa atagatgctg gctccacgga ctaaaataat agtaaggagg
     8701 aaagaggaaa ggtccaggat tgttgttcct ggatcaatat tgttccaaca ttttaaagtg
     8761 ttccaacatt ttaaaacata tgtacttagg cataaatcat catccggtat gaggaactgg
     8821 atgcctccac tctctatgac taaatccaaa ctgtcaaaac cagaatttat gtacttcttt
     8881 agaacactac gtaaatgatc aatatttaat tgtagctggg gctcagtagt ctgggctgtg
     8941 aacagatctt atttgaaacc agtatttcat acaaacttgt aaatataaag aaagccaaat
     9001 ccaagaactt aagagtattt gaattccaaa tgaagaaagg aaagaatatc ttattttgtt
     9061 ctgctttatg tttgtcttta ttcatgacca tttcttacta aaaatcacca tctgaagctt
     9121 ttgaaactcc tgttgtatgc acagcatttt aagtgcagcc atgaaataag ttagttgaaa
     9181 caaaataaaa tatctccttc cagta
//
