LOCUS       NM_133459               6271 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1),
            mRNA.
ACCESSION   NM_133459 XM_166291
VERSION     NM_133459.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Chen,C., Tang,D., Xu,S., Xiang,L., Wang,B., Yao,Y., Li,Z., Lin,S.,
            Li,S., Shi,X., Gu,C. and Gao,W.
  TITLE     The promotion of non-small cell lung cancer progression by collagen
            and calcium binding EGF domain 1 is mediated through the regulation
            of ERK/JNK/P38 phosphorylation by reactive oxygen species
  JOURNAL   Mol Carcinog 63 (8), 1467-1485 (2024)
   PUBMED   38726928
  REMARK    GeneRIF: The promotion of non-small cell lung cancer progression by
            collagen and calcium binding EGF domain 1 is mediated through the
            regulation of ERK/JNK/P38 phosphorylation by reactive oxygen
            species.
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Wang,M., Xia,D., Xu,D., Yin,Y., Xu,F., Zhang,B., Li,K., Yang,Z. and
            Zou,J.
  TITLE     Neovascularization directed by CAVIN1/CCBE1/VEGFC confers
            TMZ-resistance in glioblastoma
  JOURNAL   Cancer Lett 582, 216593 (2024)
   PUBMED   38092144
  REMARK    GeneRIF: Neovascularization directed by CAVIN1/CCBE1/VEGFC confers
            TMZ-resistance in glioblastoma.
REFERENCE   3  (bases 1 to 6271)
  AUTHORS   Song,J., Dang,X., Shen,X., Liu,Y., Gu,J., Peng,X., Huang,Z.,
            Hong,W., Cui,L. and Liu,C.Y.
  TITLE     The YAP-TEAD4 complex promotes tumor lymphangiogenesis by
            transcriptionally upregulating CCBE1 in colorectal cancer
  JOURNAL   J Biol Chem 299 (4), 103012 (2023)
   PUBMED   36781122
  REMARK    GeneRIF: The YAP-TEAD4 complex promotes tumor lymphangiogenesis by
            transcriptionally upregulating CCBE1 in colorectal cancer.
REFERENCE   4  (bases 1 to 6271)
  AUTHORS   Tian,G.A., Xu,W.T., Zhang,X.L., Zhou,Y.Q., Sun,Y., Hu,L.P.,
            Jiang,S.H., Nie,H.Z., Zhang,Z.G., Zhu,L., Li,J., Yang,X.M. and
            Yao,L.L.
  TITLE     CCBE1 promotes mitochondrial fusion by inhibiting the TGFbeta-DRP1
            axis to prevent the progression of hepatocellular carcinoma
  JOURNAL   Matrix Biol 117, 31-45 (2023)
   PUBMED   36849082
  REMARK    GeneRIF: CCBE1 promotes mitochondrial fusion by inhibiting the
            TGFbeta-DRP1 axis to prevent the progression of hepatocellular
            carcinoma.
REFERENCE   5  (bases 1 to 6271)
  AUTHORS   Ke,Y., Su,S., Duan,C., Wang,Y., Cao,G., Fang,Z., Tuo,Y., Li,W.,
            Wang,Z. and Zhang,S.
  TITLE     Hsa_circ_0076931 suppresses malignant biological properties,
            down-regulates miR-6760-3p through direct binding, and up-regulates
            CCBE1 in glioma
  JOURNAL   Biosci Rep 42 (1) (2022)
   PUBMED   34931668
  REMARK    GeneRIF: Hsa_circ_0076931 suppresses malignant biological
            properties, down-regulates miR-6760-3p through direct binding, and
            up-regulates CCBE1 in glioma.
REFERENCE   6  (bases 1 to 6271)
  AUTHORS   Connell,F., Kalidas,K., Ostergaard,P., Brice,G., Homfray,T.,
            Roberts,L., Bunyan,D.J., Mitton,S., Mansour,S., Mortimer,P. and
            Jeffery,S.
  CONSRTM   Lymphoedema Consortium
  TITLE     Linkage and sequence analysis indicate that CCBE1 is mutated in
            recessively inherited generalised lymphatic dysplasia
  JOURNAL   Hum Genet 127 (2), 231-241 (2010)
   PUBMED   19911200
  REMARK    GeneRIF: Homozygous cysteine to serine change and SNPS in CCBE1
            were identified patients.
            Erratum:[Hum Genet. 2010 Feb;127(2):243]
REFERENCE   7  (bases 1 to 6271)
  AUTHORS   Alders,M., Hogan,B.M., Gjini,E., Salehi,F., Al-Gazali,L.,
            Hennekam,E.A., Holmberg,E.E., Mannens,M.M., Mulder,M.F.,
            Offerhaus,G.J., Prescott,T.E., Schroor,E.J., Verheij,J.B.,
            Witte,M., Zwijnenburg,P.J., Vikkula,M., Schulte-Merker,S. and
            Hennekam,R.C.
  TITLE     Mutations in CCBE1 cause generalized lymph vessel dysplasia in
            humans
  JOURNAL   Nat Genet 41 (12), 1272-1274 (2009)
   PUBMED   19935664
  REMARK    GeneRIF: Mutations in CCBE1 cause generalized lymph vessel
            dysplasia in humans
REFERENCE   8  (bases 1 to 6271)
  AUTHORS   Hogan,B.M., Bos,F.L., Bussmann,J., Witte,M., Chi,N.C., Duckers,H.J.
            and Schulte-Merker,S.
  TITLE     Ccbe1 is required for embryonic lymphangiogenesis and venous
            sprouting
  JOURNAL   Nat Genet 41 (4), 396-398 (2009)
   PUBMED   19287381
REFERENCE   9  (bases 1 to 6271)
  AUTHORS   Uhl,G.R., Liu,Q.R., Drgon,T., Johnson,C., Walther,D., Rose,J.E.,
            David,S.P., Niaura,R. and Lerman,C.
  TITLE     Molecular genetics of successful smoking cessation: convergent
            genome-wide association study results
  JOURNAL   Arch Gen Psychiatry 65 (6), 683-693 (2008)
   PUBMED   18519826
  REMARK    GeneRIF: Clinical trial and genome-wide association study of
            gene-disease association, gene-environment interaction, and
            pharmacogenomic / toxicogenomic. (HuGE Navigator)
REFERENCE   10 (bases 1 to 6271)
  AUTHORS   Browning,S.R. and Thomas,J.
  TITLE     Multilocus analysis of GAW15 NARAC chromosome 18 case-control data
  JOURNAL   BMC Proc 1 Suppl 1 (Suppl 1), S11 (2007)
   PUBMED   18466450
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010776.10, AB075863.1 and
            AC016229.10.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 10, 2018 this sequence version replaced NM_133459.3.
            
            Summary: This gene is thought to function in extracellular matrix
            remodeling and migration. It is predominantly expressed in the
            ovary, but down regulated in ovarian cancer cell lines and primary
            carcinomas, suggesting its role as a tumour suppressor. Mutations
            in this gene have been associated with Hennekam
            lymphangiectasia-lymphedema syndrome, a generalized lymphatic
            dysplasia in humans. [provided by RefSeq, Mar 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB075863.1, AY358347.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000439986.9/ ENSP00000404464.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                AC010776.10        18861-18903
            44-3293             AB075863.1         1-3250
            3294-6271           AC016229.10        39681-42658
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.32"
     gene            1..6271
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /note="collagen and calcium binding EGF domains 1"
                     /db_xref="GeneID:147372"
                     /db_xref="HGNC:HGNC:29426"
                     /db_xref="MIM:612753"
     exon            1..212
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1302
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /note="collagen and calcium-binding EGF domain-containing
                     protein 1; full of fluid protein homolog"
                     /codon_start=1
                     /product="collagen and calcium-binding EGF
                     domain-containing protein 1 precursor"
                     /protein_id="NP_597716.1"
                     /db_xref="CCDS:CCDS32838.1"
                     /db_xref="GeneID:147372"
                     /db_xref="HGNC:HGNC:29426"
                     /db_xref="MIM:612753"
                     /translation="MVPPPPSRGGAARGQLGRSLGPLLLLLALGHTWTYREEPEDGDR
                     EICSESKIATTKYPCLKSSGELTTCYRKKCCKGYKFVLGQCIPEDYDVCAEAPCEQQC
                     TDNFGRVLCTCYPGYRYDRERHRKREKPYCLDIDECASSNGTLCAHICINTLGSYRCE
                     CREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQK
                     IALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGSPGPKGSPGFPGMPGPP
                     GQPGPRGSMGPMGPSPDLSHIKQGRRGPVGPPGAPGRDGSKGERGAPGPRGSPGPPGS
                     FDFLLLMLADIRNDITELQEKVFGHRTHSSAEEFPLPQEFPSYPEAMDLGSGDDHPRR
                     TETRDLRAPRDFYP"
     exon            213..293
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            294..346
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            347..481
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            482..634
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            635..735
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            736..856
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..996
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1032
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1068
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..6271
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2043..2048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /note="hexamer: ATTAAA"
     polyA_site      2065
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /note="major polyA site"
     regulatory      6253..6258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /note="hexamer: AACAAA"
     polyA_site      6271
                     /gene="CCBE1"
                     /gene_synonym="HKLLS1"
                     /note="major polyA site"
ORIGIN      
        1 gaaggcggcg gcggccgcgg aggagcagga cgcttggtcc ggacggagct cggcgctggg
       61 aagaagccgg gagcttccct gatggtgccg ccgcctccga gccggggagg agctgccagg
      121 ggccagctgg gcaggagcct gggtccgctg ctgctgctcc tggcgttggg acacacgtgg
      181 acctacagag aggagccgga ggacggcgac agagaaatct gctcagagag caaaatcgcg
      241 acgactaaat acccgtgtct gaagtcttca ggcgagctca ccacatgcta caggaaaaag
      301 tgctgcaaag gatataaatt tgttcttgga caatgcatcc cagaagatta cgacgtttgt
      361 gccgaggctc cctgtgaaca gcagtgcacg gacaactttg gccgagtgct gtgtacttgt
      421 tatccgggat accgatatga ccgggagaga caccggaagc gggagaagcc atactgtctg
      481 gatattgatg agtgtgccag cagcaatggg acgctgtgtg cccacatctg catcaatacc
      541 ttgggcagct accgctgcga gtgccgggaa ggctacatcc gggaagatga tgggaagaca
      601 tgtaccaggg gagacaaata tcccaatgac actggccatg agaagtctga gaacatggtg
      661 aaagccggaa cttgctgtgc cacatgcaag gagttctacc agatgaagca gaccgtgctg
      721 cagctgaagc aaaagattgc tctgctcccc aacaatgcag ctgacctggg caagtatatc
      781 actggtgaca aggtgctggc ctcaaacacc taccttccag gacctcctgg cctgcctggg
      841 ggccagggcc ctcccggctc accaggacca aagggaagcc caggcttccc cggtatgcca
      901 ggccctcctg ggcagcccgg cccacggggc tcaatgggac ccatgggacc atctcctgat
      961 ctgtcccaca ttaagcaagg ccggaggggc cctgtgggtc caccaggggc accaggaaga
     1021 gatggttcta agggggagag aggagcgcct gggcccagag ggtctccagg accccctggt
     1081 tctttcgact tcctgctact tatgctggct gacatccgca atgacatcac tgagctgcag
     1141 gaaaaggtgt tcgggcaccg gactcactct tcagcagagg agttcccttt acctcaggaa
     1201 tttcccagct acccagaagc catggacctg ggctctggag atgaccatcc aagaagaact
     1261 gagacaagag acttgagagc ccccagagac ttctacccat agcacatccc aacaccgtca
     1321 cgccaaagga agagaaagat caactcacct gcagttaaac catctaaaga gaagaaagac
     1381 cactggagac ctagaaaaca tacatttttc tcttctcttc tcctgacgtc tctccactcc
     1441 tcttcttcca aatacgatgc tattttcaga gtcccctcct aggcctgcag acatgaggga
     1501 gtgaatgatt gatttacctg cttctcacta agagtccatt ggggtggttt gcattgtaac
     1561 ttttctttta catcctattt ttccaggaac tttggattta agtactctca cagtgtctta
     1621 aatcataaat tcttgaagtt aaatttggca gagtatcaaa agggggaaaa tgacaaagtg
     1681 agctctaaga aaatgtgagg ctacttctaa gatgtgtgtt cacaatagac cataactcct
     1741 ctagtatcaa aattggggct cttcagttaa aaaggggtgg ggaggacaaa cgtgtcgatg
     1801 tgctttggtg gagaattttt tccttgtgct tctagtagac tttaaatatt gtatcccttt
     1861 gtcaaacctt gtttcccaaa ttcaattaaa gagaggagag aattgaatgg cgtttagaga
     1921 agatagaaaa gaatcacagt catatattta ctgttatata gattgccaca ttctaaaatt
     1981 caaatacggt gcttaaggtt tcatgccatg cttatctgta agtatcctat ttagggaaga
     2041 agattaaact ctcttttcaa aaaaacaaag tgaaatgcct ggattcacat taaaacaatg
     2101 ggctctcgtt tgctataata ttttaaagct gtttaatcaa cagtggagtc tgctctataa
     2161 atatagatta tttgttcaat aaactggctg agcttagaga gaggtgcaga attcctggtt
     2221 ctgagcaggt gcccagaagg taccattagg tgccatgatc caggctgaac caatatacag
     2281 tggggctgaa gtctgcaagg aggttgctgg cttgggctga cctcactaat gccatcagca
     2341 gcggtaggta aattttttct ccttgggtat tacaagtttt tgtctggagc caaccaagct
     2401 tgccaccaac atattgagag taatacacta ttgaaagtta tcttggatgg ggagaaaaaa
     2461 aaatagtggt tttccttgtt tgcaaaaact tccttcctat tctcattttt tcttaatttt
     2521 ctttaattta gtccaagttc cagttctttt aggccttctc tttgatttat tttcccctgc
     2581 atgtgagaag cagttcagaa aaaggtctat atctccacct cctagtgagt tagagtgttt
     2641 tctcagagca cctctgggtg gcaaagggaa gcatgttcct gccaaggttt gctgtggatt
     2701 cagaagcacc aggagcaaga gaccagaagg atgatctgct cctttgtaac gttgttgagg
     2761 gccctcttgt ttccaatgag cagcttatag gttactcaca gtccactttc tcactggaca
     2821 cacaaagtgg ctctttatct acctttgcgg gagattttca ctctcctgca aatgatcgtt
     2881 ctcacactca tattagctca tgttggaatt tcccatcctg ccatgtcctt tcccatttct
     2941 ttttggcttt tttgcctcca ccttttagcc cacatcattt aactccacta ctgtgaaagc
     3001 ttgcttaaag aaaatccctc ttggccgggt gtggtagccc acgcctctaa tcccagcact
     3061 ttgggaggct gaggcgggga gatcacaagg tcaggagatc gagaccagcc tgaccaacat
     3121 ggtgaaaccc tgtctctact aaaaatacaa aaattagctg ggcgtgttgg cacacacctg
     3181 taatcccagc tactcaggag gctgaggcag gagaattact ttaacctgcg gggggagcct
     3241 agattgcgct actgcactcc agcctaggca acagagggag actctgtctc attaaaaaaa
     3301 aaaaaaaaaa aaaaaaaagg ccgggcttgg tggctcatgc ctgtaatccc agcactttgg
     3361 gaggccgagg cgggcggatc acgaggtcag gagatcgaga ccacagtgaa accctgtctc
     3421 tactaaaaac acaaaaaatt agtcgggcgt ggtggtgggc gcctgtggtc ccagctgctc
     3481 gggaggctga ggcaggagaa tggcgtgaac ctgggaggcg gagcttgcgc tgagccgaga
     3541 tcgtgccact gcactccagc ctgggcgaca gagcgagact ctgtctcaaa aaaaaaaaaa
     3601 tatccctttt gttgtgtttg aatgccacct ttcagtcttc ttccttaaga atgttaggaa
     3661 ggggctttgc aaacctctag tggcaacagt cttccttccc ttcttcctac cacgttggaa
     3721 atgcaggact catgtcagtc agcatgtgac cgagtctttc atgaacactt tgctcctcta
     3781 tgtctcattt atgcaaaatt cctggtgact ctccactaac atttttaaag tgaaagcaaa
     3841 ctttcacttt aaaaaaagtg agcgagagga tcccttgagc ccaggagttc aaggccagtc
     3901 tgggcaacat agcaagaccc catccctaaa aaaaaaaaaa taagttttat taaatgtggc
     3961 actttcccct agaagggcag taaccccagg aaccagtctg cctcttttcg aataaaggag
     4021 actttttaaa aagaaaaaat ttctttttag ggtggggtgc tctttatcta gcatctttcc
     4081 ttcctccttc tctctgcaaa attcaacatt catgacagag ggccacacca accacgggtt
     4141 tatagagaga ggaactgaac agctgttgat tcccatctgg aatatattta atgtttgcct
     4201 gcttgtggag catggtattt aaagggtgtg gatggatgaa tgtttaaaaa aaaacacaca
     4261 aaactatgca caagctgtat gagagatggc taggtccatc tatgattttt tttaaaagaa
     4321 gaattttaat accttttctt tgacagtgaa ctgtatttag aaatatgatc tttattgtga
     4381 aatgctcata aaatgcaacc aaactgcatg tttggccata ggctagaata atatattaaa
     4441 agaattaatg gaaagtagta ttgtctgggt ttcatgaaac tattccagat aatattctcc
     4501 agcaaaactt atggtaacct gtattttata atgtcaatct gctggaccaa gttaatttag
     4561 aaaataattt ttaatcctca gattttcaaa acttggacta ctgcaagact gatatgtact
     4621 ttttatgccc aaatcatatt tttcaaagat aggacatcat aaggcatcat tatttctaat
     4681 gtatctttta actagataga aaaagcttta ctgtctaaac aggacttctg ctcaaaacct
     4741 aatgggaaag ctatttctgg ggtaagattt gtaacagtgc ttctaggtca tttcatgttt
     4801 tatagggaaa aagatctttg tacacctcct caacctgtta gtagaaatgt tgacttcgat
     4861 tttttgcacc ctcttcacag gacctcataa gttgcctctg ttcttggaaa gcaatgggaa
     4921 taaaaatatg ctgttgaaaa tagtgcatgg ggccaggcac agtggttatg cctgtaatcc
     4981 caacactttg ggaggctgag gcgggcgcat catgaggtca ggagatcaag accatcctga
     5041 ccaacacgtg gtgaaacccc atctctacta aaataaataa aaaaaaaaat tagccaggcg
     5101 tggtggtgca cgcctgtagt ctcagctact cgggaagctg agacacaaga attgcttgaa
     5161 cccgggaggt ggaggttgca gtgagccaag attgcgccac tgcactccag cctggctaca
     5221 gagtgagact ccgtctcgaa aaaagaaaat agtacatgga gggcatgcat tttagtttag
     5281 gaaacccccc tcccttccca gttccagagg gagaaattat aagctatact gtgccaaaag
     5341 tgagtggggc tgcagagaga atggtgcctt ttctcacctt ctgagttaat gcccgacatc
     5401 tgccattgga ttcattgacc aaaggaagtc atttctactg tataaactca ggagtatcat
     5461 agcccaacaa gtaaataata aatgttcatt ggttttggaa ggaaggaaag gtgagtatta
     5521 ccccaccaaa taccagcgaa gagcaccctg gctttggcca tctttccaag gttcataatt
     5581 gctgtgggcc tggtgagccc tgttcccagg tatgcaaggg agggcttctc caggccagac
     5641 atttctgtag gttttcaggt agcaactccc ccatcatatt ttgggtgtca ttccccactc
     5701 tctctcccca cttctcagcc tgtgccccag aaacaaccac agcagcctca ttctgcagac
     5761 ttaacatctg tcaaataacc ttcctgaaaa ggaaagttat tggaggaatc caggccaaac
     5821 aacaagtaga atagagccat ctaaagttat taataatgag ttcagggaaa tagatgaatt
     5881 ttttcctggc atagtcaata tttatatctc acaacaagtc aaaaataatt ttccatttgg
     5941 aaaacaaaac acaccatgac tgtaattgtt aaggcatata atcatgaact tcacatgctc
     6001 cctcaacaaa cgttactgct ctagggaaat catagaaaag gaccctctta ctccccaggg
     6061 gatcaaagca agtcccctta ggaatttcat gtgtgccgtg ggtctgtttt tatttcacag
     6121 tgactagtca tatactggag tagctctgtt gttcattatt tgtaacaggt ccatgtaaca
     6181 gtcaagttag cattcaagca attatggatg tgatactatg atgtactttt gttatgattg
     6241 tatatgctta ataacaaagt tatttttctt a
//
