LOCUS       NM_003655               2675 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens chromobox 4 (CBX4), mRNA.
ACCESSION   NM_003655
VERSION     NM_003655.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wang,J., Zhu,X., Yu,Y., Ge,J., Chen,W., Xu,W. and Zhou,W.
  TITLE     CBX4/miR-190 regulatory loop inhibits lung cancer metastasis
  JOURNAL   Thorac Cancer 15 (26), 1889-1896 (2024)
   PUBMED   39098997
  REMARK    GeneRIF: CBX4/miR-190 regulatory loop inhibits lung cancer
            metastasis.
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Zhao,R., Guo,Y., Zhang,L., Huang,Z., Li,X., Lan,B., Zhong,D.,
            Chen,H. and Xuan,C.
  TITLE     CBX4 plays a bidirectional role in transcriptional regulation and
            lung adenocarcinoma progression
  JOURNAL   Cell Death Dis 15 (5), 378 (2024)
   PUBMED   38816356
  REMARK    GeneRIF: CBX4 plays a bidirectional role in transcriptional
            regulation and lung adenocarcinoma progression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2675)
  AUTHORS   Huo,X.S., Lu,D., Chen,D.G., Ye,M., Wang,X.W. and Shang,F.S.
  TITLE     METTL3 Promotes Osteosarcoma Metastasis via an m6A-dependent
            Epigenetic Activity of CBX4
  JOURNAL   Front Biosci (Landmark Ed) 29 (3), 120 (2024)
   PUBMED   38538251
  REMARK    GeneRIF: METTL3 Promotes Osteosarcoma Metastasis via an
            m6A-dependent Epigenetic Activity of CBX4.
REFERENCE   4  (bases 1 to 2675)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   5  (bases 1 to 2675)
  AUTHORS   Dou,Z., Lu,F., Hu,J., Wang,H., Li,B. and Li,X.
  TITLE     MicroRNA-6838-5p suppresses the self-renewal and metastasis of
            human liver cancer stem cells through downregulating CBX4
            expression and inactivating ERK signaling
  JOURNAL   Biol Chem 404 (1), 29-39 (2022)
   PUBMED   36215729
  REMARK    GeneRIF: MicroRNA-6838-5p suppresses the self-renewal and
            metastasis of human liver cancer stem cells through downregulating
            CBX4 expression and inactivating ERK signaling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2675)
  AUTHORS   Sewalt,R.G., Gunster,M.J., van der Vlag,J., Satijn,D.P. and
            Otte,A.P.
  TITLE     C-Terminal binding protein is a transcriptional repressor that
            interacts with a specific class of vertebrate Polycomb proteins
  JOURNAL   Mol Cell Biol 19 (1), 777-787 (1999)
   PUBMED   9858600
REFERENCE   7  (bases 1 to 2675)
  AUTHORS   Satijn,D.P. and Otte,A.P.
  TITLE     RING1 interacts with multiple Polycomb-group proteins and displays
            tumorigenic activity
  JOURNAL   Mol Cell Biol 19 (1), 57-68 (1999)
   PUBMED   9858531
REFERENCE   8  (bases 1 to 2675)
  AUTHORS   Alkema,M.J., Jacobs,J., Voncken,J.W., Jenkins,N.A., Copeland,N.G.,
            Satijn,D.P., Otte,A.P., Berns,A. and van Lohuizen,M.
  TITLE     MPc2, a new murine homolog of the Drosophila polycomb protein is a
            member of the mouse polycomb transcriptional repressor complex
  JOURNAL   J Mol Biol 273 (5), 993-1003 (1997)
   PUBMED   9367786
REFERENCE   9  (bases 1 to 2675)
  AUTHORS   Satijn,D.P., Olson,D.J., van der Vlag,J., Hamer,K.M.,
            Lambrechts,C., Masselink,H., Gunster,M.J., Sewalt,R.G., van
            Driel,R. and Otte,A.P.
  TITLE     Interference with the expression of a novel human polycomb protein,
            hPc2, results in cellular transformation and apoptosis
  JOURNAL   Mol Cell Biol 17 (10), 6076-6086 (1997)
   PUBMED   9315667
REFERENCE   10 (bases 1 to 2675)
  AUTHORS   Satijn,D.P., Gunster,M.J., van der Vlag,J., Hamer,K.M., Schul,W.,
            Alkema,M.J., Saurin,A.J., Freemont,P.S., van Driel,R. and Otte,A.P.
  TITLE     RING1 is associated with the polycomb group protein complex and
            acts as a transcriptional repressor
  JOURNAL   Mol Cell Biol 17 (7), 4105-4113 (1997)
   PUBMED   9199346
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC100791.15, BQ225831.1, AF013956.1, BG029608.1, BC014967.1 and
            AI796748.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003655.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.687891.1,
                                           SRR1163658.308209.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2153031
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000269397.9/ ENSP00000269397.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AC100791.15        77639-77664         c
            27-189              BQ225831.1         1-163
            190-597             AF013956.1         167-574
            598-790             BG029608.1         404-596
            791-1561            AF013956.1         762-1532
            1562-2536           BC014967.1         582-1556
            2537-2675           AI796748.1         7-145               c
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..2675
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /note="chromobox 4"
                     /db_xref="GeneID:8535"
                     /db_xref="HGNC:HGNC:1554"
                     /db_xref="MIM:603079"
     exon            1..258
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..1872
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /note="chromobox homolog 4 (Pc class homolog, Drosophila);
                     Pc class 2 homolog; NS5ATP1-binding protein 16; polycomb 2
                     homolog; chromobox protein homolog 4; chromobox-like
                     protein 4"
                     /codon_start=1
                     /product="E3 SUMO-protein ligase CBX4"
                     /protein_id="NP_003646.2"
                     /db_xref="CCDS:CCDS32758.1"
                     /db_xref="GeneID:8535"
                     /db_xref="HGNC:HGNC:1554"
                     /db_xref="MIM:603079"
                     /translation="MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEP
                     EENILDPRLLIAFQNRERQEQLMGYRKRGPKPKPLVVQVPTFARRSNVLTGLQDSSTD
                     NRAKLDLGAQGKGQGHQYELNSKKHHQYQPHSKERAGKPPPPGKSGKYYYQLNSKKHH
                     PYQPDPKMYDLQYQGGHKEAPSPTCPDLGAKSHPPDKWAQGAGAKGYLGAVKPLAGAA
                     GAPGKGSEKGPPNGMMPAPKEAVTGNGIGGKMKIVKNKNKNGRIVIVMSKYMENGMQA
                     VKIKSGEVAEGEARSPSHKKRAADERHPPADRTFKKAAGAEEKKVEAPPKRREEEVSG
                     VSDPQPQDAGSRKLSPTKEAFGEQPLQLTTKPDLLAWDPARNTHPPSHHPHPHPHHHH
                     HHHHHHHHAVGLNLSHVRKRCLSETHGEREPCKKRLTARSISTPTCLGGSPAAERPAD
                     LPPAAALPQPEVILLDSDLDEPIDLRCVKTRSEAGEPPSSLQVKPETPASAAVAVAAA
                     AAPTTTAEKPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV"
     exon            259..302
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /inference="alignment:Splign:2.1.0"
     exon            303..368
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /inference="alignment:Splign:2.1.0"
     exon            369..435
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /inference="alignment:Splign:2.1.0"
     exon            436..2675
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2651..2656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /note="hexamer: ATTAAA"
     polyA_site      2675
                     /gene="CBX4"
                     /gene_synonym="NBP16; PC2"
                     /note="major polyA site"
ORIGIN      
        1 agttgtctga gcgagcgcga gccgggagcc ggggcgggcg cgggcagcgg cgggccggcc
       61 gggctgtgcg gggcgagcgg cggcggcggc gggggcgctt cggccggggc ggcagctggg
      121 cgccggcggg agctagcagc gtctgcagcc gcgccggccg ccagcgcccc ggcgcgctcc
      181 ggctcggcca tggagctgcc agctgttggc gagcacgtct tcgcggtgga gagcatcgag
      241 aagaagcgga tccgcaaggg cagagtggag tatctggtga aatggagagg ctggtcgccc
      301 aaatataaca cgtgggaacc ggaggagaac atcctggacc ccaggctgct gatcgccttc
      361 cagaacaggg aacggcagga gcagctgatg ggatatcgga agagagggcc gaagcccaaa
      421 ccgctagtgg tgcaggtgcc tacctttgcc cgtcgttcca atgtcctgac cggcctccag
      481 gactcctcca ctgacaaccg tgccaagctg gatttgggcg cgcaggggaa gggccagggg
      541 catcagtacg agctcaacag caagaagcac caccagtacc agccgcacag caaggagcgg
      601 gcgggcaagc ccccgccgcc gggcaagagc ggcaagtact actaccagct caacagcaag
      661 aagcaccacc cctaccagcc cgaccccaaa atgtacgacc tgcagtacca gggcggccac
      721 aaggaggcgc ccagccccac ctgcccggac ctgggggcca agagccaccc gcccgacaag
      781 tgggcgcaag gtgcgggggc caaaggctac ctgggggcgg tgaagcccct ggccggtgcg
      841 gcgggtgctc caggcaaagg ctccgagaag ggccccccca acggaatgat gccggccccc
      901 aaagaggctg tgacgggcaa cgggattggg ggcaagatga agatagtcaa gaacaagaac
      961 aagaacggac gcatcgtgat cgtgatgagc aaatacatgg agaacggcat gcaggcggtg
     1021 aagatcaagt ccggcgaggt ggcagagggg gaggctcgct cccccagcca caagaagcgg
     1081 gcagccgacg agcgccaccc tcctgccgac aggactttta aaaaggcggc gggcgcagag
     1141 gagaagaagg tggaggcgcc gcccaagagg agggaggagg aggtgtccgg ggttagcgat
     1201 ccgcagcccc aggatgccgg ctcccgcaag ctgtccccga ccaaggaggc ctttggagag
     1261 cagcccctgc agctcaccac caagcccgac ctgcttgcct gggacccggc ccggaacacg
     1321 cacccgccct cacaccaccc gcacccgcac ccccatcacc accaccacca ccaccaccac
     1381 caccaccacg ccgtcggcct gaatctctcc cacgtgcgca agcgctgcct ctccgagacc
     1441 cacggcgagc gcgagccctg caagaagcgg ctgactgcgc gcagcatcag cacccccacc
     1501 tgcctggggg gcagcccagc cgctgagcgc ccggccgacc tgccaccagc cgccgccctc
     1561 ccgcagcccg aggtcatcct gctagactca gacctggatg aacccataga cttgcgctgc
     1621 gtcaagacgc gcagcgaggc cggggagccg cccagctccc tccaggtgaa gcccgagaca
     1681 ccggcgtcgg cggcggtggc ggtggcggcg gcagcggcac ccaccacgac ggcggagaag
     1741 cctccagccg aggcccagga cgaacctgca gagtcgctga gcgagttcaa gcccttcttt
     1801 gggaatataa ttatcaccga cgtcaccgcg aactgcctca ccgttacttt caaggagtac
     1861 gtgacggtgt agccggaggg cgtcggaagg ggaagcgcca ttcccgcggg ggggcgggga
     1921 gctgagcacc tggggcctcg gggcgggctc ccctctcgcc aacccgccaa ccgcgagaga
     1981 cccaggctgg cccccagggt gaggacgccc ggagcggagg taaccatgtt ccccctgcgg
     2041 cggctgtcag acctgggcgg aggccccttc cacgcggtgc cggcggggct cgccctctcc
     2101 tgcccttccc cgctggagat ggacccccgg aacggacagg gcagctctgc gcccggcctc
     2161 agagttctag tattatattt taaccgtgct aacttgtcaa gtgctgactc tactcccgtt
     2221 tgtacgtggt gttattattg aaatgtattg tttgagctca aaaggcccga ccacccccct
     2281 tcgggctgct atatatatat ttatttgtag gtatttatat attgaaatat aaaaacctag
     2341 atttatggag tttcctctag atcatgttat attctatatc agacaaacta ttttcttttg
     2401 acctttcttc ccctccatcc agtatttcgg ttgatttcat tttctcccct ctcttcccct
     2461 tccacgaact gcaataccag taaccttggt atatattttt tgatactgta cacatggatg
     2521 tcttgtttct atgtgcaaaa aaaaaaaaaa aaaaaagttt gttaaaaggc tacacgagct
     2581 ctctagaaac tgctgctact agaaatgtct aaactataag cttccaacta ttacctgctt
     2641 gaatgtaaat attaaatgga gatgttgaag gtgca
//
