LOCUS       NM_170662               6749 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens Cbl proto-oncogene B (CBLB), transcript variant 2,
            mRNA.
ACCESSION   NM_170662
VERSION     NM_170662.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Li,X., Sun,W., Huang,M., Gong,L., Zhang,X., Zhong,L., Calderon,V.,
            Bian,Z., He,Y., Suh,W.K., Li,Y., Song,T., Zou,Y., Lian,Z.X. and
            Gu,H.
  TITLE     Deficiency of CBL and CBLB ubiquitin ligases leads to hyper T
            follicular helper cell responses and lupus by reducing BCL6
            degradation
  JOURNAL   Immunity 57 (7), 1603-1617 (2024)
   PUBMED   38761804
  REMARK    GeneRIF: Deficiency of CBL and CBLB ubiquitin ligases leads to
            hyper T follicular helper cell responses and lupus by reducing BCL6
            degradation.
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Li,S., Wan,L., Liu,J., Huang,C., Chen,Y., Cheng,J., Hu,S., Li,F.
            and Zhu,Z.
  TITLE     [Circular RNA Cbl proto-oncogene B (circCBLB) inhibits
            proliferation, promotes apoptosis, and increases anti-inflammatory
            cytokine levels of fibroblast-like synoviocytes in rheumatoid
            arthritis]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 40 (2), 106-113 (2024)
   PUBMED   38284251
  REMARK    GeneRIF: [Circular RNA Cbl proto-oncogene B (circCBLB) inhibits
            proliferation, promotes apoptosis, and increases anti-inflammatory
            cytokine levels of fibroblast-like synoviocytes in rheumatoid
            arthritis].
REFERENCE   3  (bases 1 to 6749)
  AUTHORS   Pinilla-Macua,I. and Sorkin,A.
  TITLE     Cbl and Cbl-b independently regulate EGFR through distinct receptor
            interaction modes
  JOURNAL   Mol Biol Cell 34 (13), ar134 (2023)
   PUBMED   37903221
  REMARK    GeneRIF: Cbl and Cbl-b independently regulate EGFR through distinct
            receptor interaction modes.
REFERENCE   4  (bases 1 to 6749)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6749)
  AUTHORS   Wirnsberger,G., Zwolanek,F., Asaoka,T., Kozieradzki,I., Tortola,L.,
            Wimmer,R.A., Kavirayani,A., Fresser,F., Baier,G., Langdon,W.Y.,
            Ikeda,F., Kuchler,K. and Penninger,J.M.
  TITLE     Inhibition of CBLB protects from lethal Candida albicans sepsis
  JOURNAL   Nat Med 22 (8), 915-923 (2016)
   PUBMED   27428901
REFERENCE   6  (bases 1 to 6749)
  AUTHORS   Xiao,Y., Tang,J., Guo,H., Zhao,Y., Tang,R., Ouyang,S., Zeng,Q.,
            Rappleye,C.A., Rajaram,M.V., Schlesinger,L.S., Tao,L., Brown,G.D.,
            Langdon,W.Y., Li,B.T. and Zhang,J.
  TITLE     Targeting CBLB as a potential therapeutic approach for disseminated
            candidiasis
  JOURNAL   Nat Med 22 (8), 906-914 (2016)
   PUBMED   27428899
  REMARK    GeneRIF: Silencing CBLB in human monocyte-derived macrophages
            resulted in increased cytokine production and impaired
            down-modulation of dectin-1 and dectin-2 expression after
            C.albicans exposure.
REFERENCE   7  (bases 1 to 6749)
  AUTHORS   Liu,Q., Zhou,H., Langdon,W.Y. and Zhang,J.
  TITLE     E3 ubiquitin ligase Cbl-b in innate and adaptive immunity
  JOURNAL   Cell Cycle 13 (12), 1875-1884 (2014)
   PUBMED   24875217
  REMARK    Review article
REFERENCE   8  (bases 1 to 6749)
  AUTHORS   Lavagna-Sevenier,C., Marchetto,S., Birnbaum,D. and Rosnet,O.
  TITLE     The CBL-related protein CBLB participates in FLT3 and interleukin-7
            receptor signal transduction in pro-B cells
  JOURNAL   J Biol Chem 273 (24), 14962-14967 (1998)
   PUBMED   9614102
REFERENCE   9  (bases 1 to 6749)
  AUTHORS   Bustelo,X.R., Crespo,P., Lopez-Barahona,M., Gutkind,J.S. and
            Barbacid,M.
  TITLE     Cbl-b, a member of the Sli-1/c-Cbl protein family, inhibits
            Vav-mediated c-Jun N-terminal kinase activation
  JOURNAL   Oncogene 15 (21), 2511-2520 (1997)
   PUBMED   9399639
REFERENCE   10 (bases 1 to 6749)
  AUTHORS   Keane,M.M., Rivero-Lezcano,O.M., Mitchell,J.A., Robbins,K.C. and
            Lipkowitz,S.
  TITLE     Cloning and characterization of cbl-b: a SH3 binding protein with
            homology to the c-cbl proto-oncogene
  JOURNAL   Oncogene 10 (12), 2367-2377 (1995)
   PUBMED   7784085
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U26710.1, BC032851.2,
            BC065915.1, AK094184.1, AC016138.8 and AI221219.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 10, 2018 this sequence version replaced NM_170662.4.
            
            Summary: This gene encodes an E3 ubiquitin-protein ligase which
            promotes proteosome-mediated protein degradation by transferring
            ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate.
            The encoded protein is involved in the regulation of immune
            response by limiting T-cell receptor, B-cell receptor, and high
            affinity immunoglobulin epsilon receptor activation. Studies in
            mouse suggest that this gene is involved in antifungal host defense
            and that its inhibition leads to increased fungal killing.
            Manipulation of this gene may be beneficial in implementing
            immunotherapies for a variety of conditions, including cancer,
            autoimmune diseases, allergies, and infections. [provided by
            RefSeq, Sep 2017].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            initiates translation from a downstream in-frame start codon,
            compared to variant 1. The encoded isoform (b) is shorter at the
            N-terminus, compared to isoform a. Both variants 2 and 3 encode
            isoform b.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.3382360.1,
                                           SRR14038197.2138584.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394030.8/ ENSP00000377598.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-918               U26710.1           32-949
            919-1562            BC032851.2         891-1534
            1563-1871           U26710.1           1594-1902
            1872-2041           BC032851.2         1844-2013
            2042-2495           U26710.1           2073-2526
            2496-2903           BC032851.2         2468-2875
            2904-3538           BC065915.1         434-1068
            3539-3595           AK094184.1         1796-1852
            3596-3815           BC032851.2         3568-3787
            3816-3816           BC065915.1         1346-1346
            3817-3817           AK094184.1         2073-2073
            3818-3936           BC032851.2         3788-3906
            3937-6364           AC016138.8         106692-109119
            6365-6749           AI221219.1         12-396              c
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.11"
     gene            1..6749
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /note="Cbl proto-oncogene B"
                     /db_xref="GeneID:868"
                     /db_xref="HGNC:HGNC:1542"
                     /db_xref="MIM:604491"
     exon            1..277
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            278..459
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     CDS             292..3240
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /EC_number="2.3.2.27"
                     /note="isoform b is encoded by transcript variant 2; E3
                     ubiquitin-protein ligase CBL-B; RING finger protein 56;
                     SH3-binding protein CBL-B; signal transduction protein
                     CBL-B; RING-type E3 ubiquitin transferase CBL-B; Cbl
                     proto-oncogene B, E3 ubiquitin protein ligase; casitas
                     B-lineage lymphoma proto-oncogene b; Cas-Br-M (murine)
                     ecotropic retroviral transforming sequence b; Cbl
                     proto-oncogene, E3 ubiquitin protein ligase B"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase CBL-B isoform b"
                     /protein_id="NP_733762.2"
                     /db_xref="CCDS:CCDS2948.1"
                     /db_xref="GeneID:868"
                     /db_xref="HGNC:HGNC:1542"
                     /db_xref="MIM:604491"
                     /translation="MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRR
                     TVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLS
                     ENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAI
                     FPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKS
                     TIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKY
                     STKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDG
                     RSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLM
                     CTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFGMPMLDLD
                     DDDDREESLMMNRLANVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVP
                     PRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPPERPPPIP
                     PDNRLSRHIHHVESVPSRDPPMPLEAWCPRDVFGTNQLVGCRLLGEGSPKPGITASSN
                     VNGRHSRVGSDPVLMRKHRRHDLPLEGAKVFSNGHLGSEEYDVPPRLSPPPPVTTLLP
                     SIKCTGPLANSLSEKTRDPVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGH
                     CMLNGTHGPSSEKKSNIPDLSIYLKGDVFDSASDPVPLPPARPPTRDNPKHGSSLNRT
                     PSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPS
                     DPFVDLASGQVPLPPARRLPGENVKTNRTSQDYDQLPSCSDGSQAPARPPKPRPRRTA
                     PEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNVEVARSILREFAF
                     PPPVSPRLNL"
     exon            460..710
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            711..857
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            858..1014
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1136
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1274
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1362
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1494
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1698
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1884
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2250
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2345
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2492
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2587
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..2719
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2860
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            2861..2980
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     exon            2981..6749
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3908..3913
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /note="hexamer: AATAAA"
     polyA_site      3941
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /note="major polyA site"
     regulatory      6733..6738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /note="hexamer: AATGAA"
     polyA_site      6749
                     /gene="CBLB"
                     /gene_synonym="ADMIO3; Cbl-b; Nbla00127; RNF56"
                     /note="major polyA site"
ORIGIN      
        1 gtccagcgag cggtctcctc ctcctgctag tgctgctgcg gcgtcccgcg gcctccccga
       61 gtcgggcggg aggggagagc gggtgtggat ttgtcttgac ggtaattgtt gcgtttccac
      121 gtctcggagg cctgcgcgct gggttgctcc ttcttcggga gcgagctgtt ctcagcgatc
      181 ccactcccag ccggggctcc ccacacacac tgggctgcgt gcgtgtggag tgggacccgc
      241 gcacacgcgt gtctctggac agctacggcg ccgaaagaac taaaattcca gatggcaaac
      301 tcaatgaatg gcagaaaccc tggtggtcga ggaggaaatc cccgaaaagg tcgaattttg
      361 ggtattattg atgctattca ggatgcagtt ggacccccta agcaagctgc cgcagatcgc
      421 aggaccgtgg agaagacttg gaagctcatg gacaaagtgg taagactgtg ccaaaatccc
      481 aaacttcagt tgaaaaatag cccaccatat atacttgata ttttgcctga tacatatcag
      541 catttacgac ttatattgag taaatatgat gacaaccaga aacttgccca actcagtgag
      601 aatgagtact ttaaaatcta cattgatagc cttatgaaaa agtcaaaacg ggcaataaga
      661 ctctttaaag aaggcaagga gagaatgtat gaagaacagt cacaggacag acgaaatctc
      721 acaaaactgt cccttatctt cagtcacatg ctggcagaaa tcaaagcaat ctttcccaat
      781 ggtcaattcc agggagataa ctttcgtatc acaaaagcag atgctgctga attctggaga
      841 aagttttttg gagacaaaac tatcgtacca tggaaagtat tcagacagtg ccttcatgag
      901 gtccaccaga ttagctctgg cctggaagca atggctctaa aatcaacaat tgatttaact
      961 tgcaatgatt acatttcagt ttttgaattt gatattttta ccaggctgtt tcagccttgg
     1021 ggctctattt tgcggaattg gaatttctta gctgtgacac atccaggtta catggcattt
     1081 ctcacatatg atgaagttaa agcacgacta cagaaatata gcaccaaacc cggaagctat
     1141 attttccggt taagttgcac tcgattggga cagtgggcca ttggctatgt gactggggat
     1201 gggaatatct tacagaccat acctcataac aagcccttat ttcaagccct gattgatggc
     1261 agcagggaag gattttatct ttatcctgat gggaggagtt ataatcctga tttaactgga
     1321 ttatgtgaac ctacacctca tgaccatata aaagttacac aggaacaata tgaattatat
     1381 tgtgaaatgg gctccacttt tcagctctgt aagatttgtg cagagaatga caaagatgtc
     1441 aagattgagc cttgtgggca tttgatgtgc acctcttgcc ttacggcatg gcaggagtcg
     1501 gatggtcagg gctgcccttt ctgtcgttgt gaaataaaag gaactgagcc cataatcgtg
     1561 gacccctttg atccaagaga tgaaggctcc aggtgttgca gcatcattga cccctttggc
     1621 atgccgatgc tagacttgga cgacgatgat gatcgtgagg agtccttgat gatgaatcgg
     1681 ttggcaaacg tccgaaagtg cactgacagg cagaactcac cagtcacatc accaggatcc
     1741 tctccccttg cccagagaag aaagccacag cctgacccac tccagatccc acatctaagc
     1801 ctgccacccg tgcctcctcg cctggatcta attcagaaag gcatagttag atctccctgt
     1861 ggcagcccaa cgggttcacc aaagtcttct ccttgcatgg tgagaaaaca agataaacca
     1921 ctcccagcac cacctcctcc cttaagagat cctcctccac cgccacctga aagacctcca
     1981 ccaatcccac cagacaatag actgagtaga cacatccatc atgtggaaag cgtgccttcc
     2041 agagacccgc caatgcctct tgaagcatgg tgccctcggg atgtgtttgg gactaatcag
     2101 cttgtgggat gtcgactcct aggggagggc tctccaaaac ctggaatcac agcgagttca
     2161 aatgtcaatg gaaggcacag tagagtgggc tctgacccag tgcttatgcg gaaacacaga
     2221 cgccatgatt tgcctttaga aggagctaag gtcttttcca atggtcacct tggaagtgaa
     2281 gaatatgatg ttcctccccg gctttctcct cctcctccag ttaccaccct cctccctagc
     2341 ataaagtgta ctggtccgtt agcaaattct ctttcagaga aaacaagaga cccagtagag
     2401 gaagatgatg atgaatacaa gattccttca tcccaccctg tttccctgaa ttcacaacca
     2461 tctcattgtc ataatgtaaa acctcctgtt cggtcttgtg ataatggtca ctgtatgctg
     2521 aatggaacac atggtccatc ttcagagaag aaatcaaaca tccctgactt aagcatatat
     2581 ttaaagggag atgtttttga ttcagcctct gatcccgtgc cattaccacc tgccaggcct
     2641 ccaactcggg acaatccaaa gcatggttct tcactcaaca ggacgccctc tgattatgat
     2701 cttctcatcc ctccattagg tgaagatgct tttgatgccc tccctccatc tctcccacct
     2761 cccccacctc ctgcaaggca tagtctcatt gaacattcaa aacctcctgg ctccagtagc
     2821 cggccatcct caggacagga tctttttctt cttccttcag atccctttgt tgatctagca
     2881 agtggccaag ttcctttgcc tcctgctaga aggttaccag gtgaaaatgt caaaactaac
     2941 agaacatcac aggactatga tcagcttcct tcatgttcag atggttcaca ggcaccagcc
     3001 agacccccta aaccacgacc gcgcaggact gcaccagaaa ttcaccacag aaaaccccat
     3061 gggcctgagg cggcattgga aaatgtcgat gcaaaaattg caaaactcat gggagagggt
     3121 tatgcctttg aagaggtgaa gagagcctta gagatagccc agaataatgt cgaagttgcc
     3181 cggagcatcc tccgagaatt tgccttccct cctccagtat ccccacgtct aaatctatag
     3241 cagccagaac tgtagacacc aaaatggaaa gcaatcgatg tattccaaga gtgtggaaat
     3301 aaagagaact gagatggaat tcaagagaga agtgtctcct cctcgtgtag cagcttgaga
     3361 agaggcttgg gagtgcagct tctcaaagga gaccgatgct tgctcaggat gtcgacagct
     3421 gtggcttcct tgtttttgct agccatattt ttaaatcagg gttgaactga caaaaataat
     3481 ttaaagacgt ttacttccct tgaactttga acctgtgaaa tgctttacct tgtttacagt
     3541 ttggcaaagt tgcagtttgt tcttgttttt agtttagttt tgttttggtg ttttgatacc
     3601 tgtactgtgt tcttcacaga ccctttgtag cgtggtcagg tctgctgtaa catttcccac
     3661 caactctctt gctgtccaca tcaacagcta aatcatttat tcatatggat ctctaccatc
     3721 cccatgcctt gcccaggtcc agttccattt ctctcattca caagatgctt tgaaggttct
     3781 gattttcaac tgatcaaact aatgcaaaaa aaaaaaagta tgtattcttc actactgagt
     3841 ttcttctttg gaaaccatca ctattgagag atgggaaaaa cctgaatgta taaagcattt
     3901 atttgtcaat aaactgcctt ttgtaagggg ttttcacata acatagagga gcttcccttt
     3961 tttgtttaag ttttgtaacc tttaatcctc catattctca tgtctgtcat cccaggggtg
     4021 tcacaactgt aaaaatctac aatattagaa agcagctact acagatgtgg aagagagaac
     4081 acttgtataa gacaatgctg tactgaagtt atataacaag gtcccctgat acttatgcct
     4141 gcattacttt gagggatgct gaatgagagg accatctccc tgaaatatat taataatttt
     4201 cagataaaat tatgaacata acataccttt tctcaagtgg aattagagtt catttcccat
     4261 ttaagtgatt acactctatc tcctaagagc tgtgggaacc agttcatatt gcagtggaaa
     4321 cattaactct tcgttgcact gttgagtgtc aaggctactg tggcaatgtt ttttgcaaag
     4381 ttctcagtaa ttttctctct gcaatcaaat tagagttcat atttaatttg cagattctct
     4441 cattcattgc tccacactgc tttatcaaat caggtgaaag aaattaatgt agttttgcct
     4501 attttacaaa atggtggttg gttctttaaa caccatgtta ttactcttaa taaatctaca
     4561 aatatttatt gagcatctat gatatgcttg gtgctgttag gcactgtgtt tttaaagcac
     4621 aaactctcaa aagctgattc cagcgtaatg ttttattttt gtttttctgt gttttttatg
     4681 acaaagtctg tctgatagac aaagcctgga aggtttcccc cagggcttta gaatttagaa
     4741 ttagctggtt ttcatttcaa atgggaaatt aatggagaaa tgagataaaa tcatctctgt
     4801 gtaaatgctt gaacccacag aaagttttct aaatatggac taattgtggg tttgtgcata
     4861 tttctgtgtc tcctcttttc acttcacacc caccatgata taaaaagatg catcagagag
     4921 tgagcctgcc ttcaggacct gaaaggacat gactgaacat ctagtagatc tggtggaaat
     4981 taatgttata caaaagacac gtagactaag ttgaccctgg gagaggagtt aatacattcc
     5041 tttgcaaagt gacatgtgcc ctttttttga cagtctggct ttcatggtat gagatgagga
     5101 ctcacaggcc ttgggtaatt tctgcttttg tatgaatttt ttcagatggt tctgagctgg
     5161 ttatgtccct ggaggggaat ttttcatttg ccttagatgt tcttcccatg agtaggtaca
     5221 tgagtgaagt ttcttacact tttaaaattg cattggcaaa ttttcttttg ctttatgaac
     5281 ccctgttttg tcattatgat ttttcacttg ttttaacagt ttcagatgtc accttggaat
     5341 agcactgttt attttggcct ttttatgatt gctgatgata ggtacagtct aaaggccaaa
     5401 agttgccact tcattgtgat aacattgttg caacattcct aatatattag agaatgatgt
     5461 aaaataagaa tgttagagaa catagcacat tctcattcag tttataaaca atatttgaaa
     5521 atttccagtg agaattaaaa aaggactagt gatgatatag caaacctgga cctgaaattt
     5581 gggggtgggg ggtgggggga tatctgtttt gcagagaaaa tttaccagcc tccagcaaaa
     5641 tctctgcatc ttgtactcaa tagcaaccat tagtattgtc atttaaaaag aatgaatcaa
     5701 aaattcatac gtaatggaat gaaaagagga aaactttcta ttaaacactg tcaagaaaga
     5761 cccattgcta tctatcacct atgaaattaa acctaaaaaa attttttttt attgtaaaag
     5821 cgactgctcg ttgatgtctg gaagtgatcc agactttgtt tcactgtgat ccaaaaagtt
     5881 agacattcct tccatttctc aagatttggt aacaattttt ttttaagtca gcatttcata
     5941 taatacataa aattaagaca aattaactta ctgatatgtg gccaatatca acccttctca
     6001 cccaacccaa acatgttctg gagatagaag gacttatcct caattatatt ccagtaaatt
     6061 tatctccata atctgtttta tcccctaatg tatgatatat ctcaccaaat ttttctagaa
     6121 ttgaataaaa gcagtttttg cttaacttga atttccttcc acaaatacaa ccttcagctc
     6181 ttccctctct gtttcccttt tgcccacatt gcaaatagtc ttcataatag aaccctcact
     6241 gcaacttaaa agggtcccat ttcttaactc agcatttcat ataccaagaa cacttgcaaa
     6301 gatttcttca taatgtgatt ttttaatttt tcagttccag aagcatttcc tacagttcga
     6361 acgttatttt ttctctcaga ttttcaactt gaagctttga tttgcactga attaacttgg
     6421 ataaaatttt agtttctctc ttcagctaac agatttggaa gcaaattaca gtatacttga
     6481 ataattcagg tactacctgt tgacctggaa ttttagtgat tctgggaaag ttttccagac
     6541 aaagacacca atagagtgaa gggttttttc cccaattact attttattgt tttttaaaat
     6601 gcctttttaa aacttgtgtg atgtggcaaa atagtcatat gttcaattaa tttatatttt
     6661 attcattcct tttcaattct ggttattatg taacctcaag tactttatct gttcttttaa
     6721 agtattttat aaaatgaacg ttaactaaa
//
