LOCUS       NM_001750               4490 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens calpastatin (CAST), transcript variant 1, mRNA.
ACCESSION   NM_001750
VERSION     NM_001750.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Yin,S., Xu,L., Yang,K., Fan,Q., Gu,Y., Yin,C., Zang,Y., Wang,Y.,
            Yuan,Y., Chang,A., Pang,C. and Ren,S.
  TITLE     Gene-Environment Interaction Between CAST Gene and Eye-Rubbing in
            the Chinese Keratoconus Cohort Study: A Case-Only Study
  JOURNAL   Invest Ophthalmol Vis Sci 65 (10), 36 (2024)
   PUBMED   39186261
  REMARK    GeneRIF: Gene-Environment Interaction Between CAST Gene and
            Eye-Rubbing in the Chinese Keratoconus Cohort Study: A Case-Only
            Study.
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Durmaz,C.D. and Tekmenuray-Unal,A.
  TITLE     Novel nonsense CAST mutation in two siblings with PLACK syndrome
  JOURNAL   Int J Dermatol 62 (10), 1295-1299 (2023)
   PUBMED   37387535
  REMARK    GeneRIF: Novel nonsense CAST mutation in two siblings with PLACK
            syndrome.
REFERENCE   3  (bases 1 to 4490)
  AUTHORS   Martins,A.C., Rieck,M., Leotti,V.B., Saraiva-Pereira,M.L. and
            Jardim,L.B.
  TITLE     Variants in Genes of Calpain System as Modifiers of Spinocerebellar
            Ataxia Type 3 Phenotype
  JOURNAL   J Mol Neurosci 71 (9), 1906-1913 (2021)
   PUBMED   34191270
  REMARK    GeneRIF: Variants in Genes of Calpain System as Modifiers of
            Spinocerebellar Ataxia Type 3 Phenotype.
REFERENCE   4  (bases 1 to 4490)
  AUTHORS   Flores-Toro,J., Chun,S.K., Shin,J.K., Campbell,J.,
            Lichtenberger,M., Chapman,W., Zendejas,I., Behrns,K.,
            Leeuwenburgh,C. and Kim,J.S.
  TITLE     Critical Roles of Calpastatin in Ischemia/Reperfusion Injury in
            Aged Livers
  JOURNAL   Cells 10 (8), 1863 (2021)
   PUBMED   34440632
  REMARK    GeneRIF: Critical Roles of Calpastatin in Ischemia/Reperfusion
            Injury in Aged Livers.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4490)
  AUTHORS   Li,S., Liang,Z.G., Wang,G.Y., Yavetz,B., Kim,E.D. and Goldberg,E.
  TITLE     Molecular cloning and characterization of functional domains of a
            human testis-specific isoform of calpastatin
  JOURNAL   Biol Reprod 63 (1), 172-178 (2000)
   PUBMED   10859257
REFERENCE   6  (bases 1 to 4490)
  AUTHORS   Lee,W.J., Ma,H., Takano,E., Yang,H.Q., Hatanaka,M. and Maki,M.
  TITLE     Molecular diversity in amino-terminal domains of human calpastatin
            by exon skipping
  JOURNAL   J Biol Chem 267 (12), 8437-8442 (1992)
   PUBMED   1569094
REFERENCE   7  (bases 1 to 4490)
  AUTHORS   Adachi,Y., Ishida-Takahashi,A., Takahashi,C., Takano,E., Murachi,T.
            and Hatanaka,M.
  TITLE     Phosphorylation and subcellular distribution of calpastatin in
            human hematopoietic system cells
  JOURNAL   J Biol Chem 266 (6), 3968-3972 (1991)
   PUBMED   1995645
REFERENCE   8  (bases 1 to 4490)
  AUTHORS   Uemori,T., Shimojo,T., Asada,K., Asano,T., Kimizuka,F., Kato,I.,
            Maki,M., Hatanaka,M., Murachi,T., Hanzawa,H. et al.
  TITLE     Characterization of a functional domain of human calpastatin
  JOURNAL   Biochem Biophys Res Commun 166 (3), 1485-1493 (1990)
   PUBMED   2407243
REFERENCE   9  (bases 1 to 4490)
  AUTHORS   Inazawa,J., Nakagawa,H., Misawa,S., Abe,T., Minoshima,S.,
            Fukuyama,R., Maki,M., Murachi,T., Hatanaka,M. and Shimizu,N.
  TITLE     Assignment of the human calpastatin gene (CAST) to chromosome 5 at
            region q14----q22
  JOURNAL   Cytogenet Cell Genet 54 (3-4), 156-158 (1990)
   PUBMED   2265559
REFERENCE   10 (bases 1 to 4490)
  AUTHORS   Asada,K., Ishino,Y., Shimada,M., Shimojo,T., Endo,M., Kimizuka,F.,
            Kato,I., Maki,M., Hatanaka,M. and Murachi,T.
  TITLE     cDNA cloning of human calpastatin: sequence homology among human,
            pig, and rabbit calpastatins
  JOURNAL   J Enzyme Inhib 3 (1), 49-56 (1989)
   PUBMED   2577276
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC020900.5, AC008906.5 and
            KC877037.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001750.6.
            
            Summary: The protein encoded by this gene is an endogenous calpain
            (calcium-dependent cysteine protease) inhibitor. It consists of an
            N-terminal domain L and four repetitive calpain-inhibition domains
            (domains 1-4), and it is involved in the proteolysis of amyloid
            precursor protein. The calpain/calpastatin system is involved in
            numerous membrane fusion events, such as neural vesicle exocytosis
            and platelet and red-cell aggregation. The encoded protein is also
            thought to affect the expression levels of genes encoding
            structural or regulatory proteins. Alternatively spliced transcript
            variants encoding different isoforms have been described. [provided
            by RefSeq, Jun 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.113174.1,
                                           SRR1660803.78729.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000675179.1/ ENSP00000501872.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AC020900.5         84544-84665         c
            123-185             AC020900.5         71440-71502         c
            186-257             AC020900.5         51134-51205         c
            258-317             AC020900.5         24343-24402         c
            318-383             AC020900.5         20182-20247         c
            384-425             AC020900.5         19511-19552         c
            426-482             AC020900.5         17832-17888         c
            483-596             AC020900.5         17316-17429         c
            597-677             AC020900.5         16181-16261         c
            678-746             AC020900.5         10801-10869         c
            747-845             AC020900.5         9094-9192           c
            846-926             AC020900.5         6923-7003           c
            927-965             AC020900.5         6258-6296           c
            966-1058            AC020900.5         5683-5775           c
            1059-1145           AC020900.5         5461-5547           c
            1146-1247           AC020900.5         4285-4386           c
            1248-1331           AC020900.5         616-699             c
            1332-1379           AC008906.5         72711-72758
            1380-1475           AC008906.5         73884-73979
            1476-1571           AC008906.5         75953-76048
            1572-1673           AC008906.5         79426-79527
            1674-1757           AC008906.5         80024-80107
            1758-1808           AC008906.5         82810-82860
            1809-1880           AC008906.5         82949-83020
            1881-1979           AC008906.5         87640-87738
            1980-2084           AC008906.5         90587-90691
            2085-2118           AC008906.5         91419-91452
            2119-2177           KC877037.1         1-59
            2178-2222           AC008906.5         92804-92848
            2223-2315           AC008906.5         93273-93365
            2316-2387           AC008906.5         95897-95968
            2388-2446           AC008906.5         97010-97068
            2447-4490           AC008906.5         98006-100049
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q15"
     gene            1..4490
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /note="calpastatin"
                     /db_xref="GeneID:831"
                     /db_xref="HGNC:HGNC:1515"
                     /db_xref="MIM:114090"
     exon            1..122
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..2423
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /note="isoform a is encoded by transcript variant 1;
                     calpain inhibitor; sperm BS-17 component; MIR583 host"
                     /codon_start=1
                     /product="calpastatin isoform a"
                     /protein_id="NP_001741.4"
                     /db_xref="GeneID:831"
                     /db_xref="HGNC:HGNC:1515"
                     /db_xref="MIM:114090"
                     /translation="MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGS
                     DEKKAASLGSSQSSRTYAGGTASATKVSASSGATSKSSSMNPTETKAIPVSQQMEGPH
                     LPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSND
                     AHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLT
                     PAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEE
                     LGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKT
                     EKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDL
                     RSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESE
                     LIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATL
                     KGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRL
                     EEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISE
                     VVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKA
                     KAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGD
                     LDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD"
     exon            123..185
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            186..257
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            258..317
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            318..383
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            384..425
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            426..482
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            483..596
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            597..677
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            678..746
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            747..845
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            846..926
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            927..965
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1058
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1145
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1247
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1331
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1379
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1475
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1571
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1673
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1757
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1808
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1880
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1979
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2084
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2177
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2222
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2315
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2387
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2446
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..4490
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2999..3004
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /note="hexamer: AATAAA"
     polyA_site      3043
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /note="major polyA site"
     regulatory      4464..4469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /note="hexamer: ATTAAA"
     polyA_site      4490
                     /gene="CAST"
                     /gene_synonym="BS-17; MIR583HG; PLACK"
                     /note="major polyA site"
ORIGIN      
        1 actctccgcg gcgcattccg ggaggcagcg gccgcagcgg cctcgccatg tcccagcccg
       61 gccagaagcc cgccgcctcc ccgcggcccc ggcgagcagc cgccgcccgc cgcacccatg
      121 agcatgtcag tgaaaaaacc agtgaatcgc cttccaaacc aggagaaaag aaaggatcag
      181 atgagaaaaa agcagcaagc ctcggcagca gtcaatcctc cagaacctat gctggtggaa
      241 cagcctcggc caccaaggtg tcagcttcct ctggtgcaac cagcaagtct tccagtatga
      301 atcccacaga aaccaaggcc attccagtca gccaacagat ggaaggacca catcttccta
      361 acaagaaaaa acacaaaaaa caggctgtaa aaacagaacc tgagaagaag tcacagtcaa
      421 ccaagctgtc tgtggttcat gagaaaaaat cccaagaagg aaagccaaaa gaacacacag
      481 agccaaaaag cctacccaag caggcatcag atacaggaag taacgatgct cacaataaaa
      541 aagcagtttc cagatcagct gaacagcagc catcagagaa atcaacagaa ccaaagacta
      601 aaccacaaga catgatttct gctggtggag agagtgttgc tggtatcact gcaatatctg
      661 gcaagccggg tgacaagaaa aaagaaaaga aatcattaac cccagctgtg ccagttgaat
      721 ctaaaccgga taaaccatcg ggaaagtcag gcatggatgc tgctttggat gacttaatag
      781 atactttagg aggacctgaa gaaactgaag aagaaaatac aacgtatact ggaccagaag
      841 tttcagatcc aatgagttcc acctacatag aggaattggg taaaagagaa gtcacaattc
      901 ctccaaaata tagggaacta ttggctaaaa aggaagggat cacagggcct cctgcagact
      961 cttcgaaacc catagggcca gatgatgcta tagacgcctt gtcatctgac ttcacctgtg
     1021 ggtcgcctac agctgctgga aagaaaactg aaaaagagga atctacagaa gttttaaaag
     1081 ctcagtcagc agggacagtc agaagtgctg ctccacccca agagaagaaa agaaaggtgg
     1141 agaaggatac aatgagtgat caagcactcg aggctctgtc ggcttcactg ggcacccggc
     1201 aagcagaacc tgagctcgac ctccgctcaa ttaaggaagt cgatgaggca aaagctaaag
     1261 aagaaaaact agagaagtgt ggtgaggatg atgaaacaat cccatctgag tacagattaa
     1321 aaccagccac ggataaagat ggaaaaccac tattgccaga gcctgaagaa aaacccaagc
     1381 ctcggagtga atcagaactc attgatgaac tttcagaaga ttttgaccgg tctgaatgta
     1441 aagagaaacc atctaagcca actgaaaaga cagaagaatc taaggccgct gctccagctc
     1501 ctgtgtcgga ggctgtgtgt cggacctcca tgtgtagtat acagtcagca ccccctgagc
     1561 cggctacctt gaagggcaca gtgccagatg atgctgtaga agccttggct gatagcctgg
     1621 ggaaaaagga agcagatcca gaagatggaa aacctgtgat ggataaagtc aaggagaagg
     1681 ccaaagaaga agaccgtgaa aagcttggtg aaaaagaaga aacaattcct cctgattata
     1741 gattagaaga ggtcaaggat aaagatggaa agccactcct gccaaaagag tctaaggaac
     1801 agcttccacc catgagtgaa gacttccttc tggatgcttt gtctgaggac ttctctggtc
     1861 cacaaaatgc ttcatctctt aaatttgaag atgctaaact tgctgctgcc atctctgaag
     1921 tggtttccca aaccccagct tcaacgaccc aagctggagc cccaccccgt gatacctcgc
     1981 agagtgacaa agacctcgat gatgccttgg ataaactctc tgacagtcta ggacaaaggc
     2041 agcctgaccc agatgagaac aaaccaatgg aagataaagt aaaggaaaaa gctaaagctg
     2101 aacatagaga caagcttgga gaaagagatg acactatccc acctgaatac agacatctcc
     2161 tggatgataa tggacaggac aaaccagtga agccacctac aaagaaatca gaggattcaa
     2221 agaaacctgc agatgaccaa gaccccattg atgctctctc aggagatctg gacagctgtc
     2281 cctccactac agaaacctca cagaacacag caaaggataa gtgcaagaag gctgcttcca
     2341 gctccaaagc acctaagaat ggaggtaaag cgaaggattc agcaaagaca acagaggaaa
     2401 cttccaagcc aaaagatgac taaagaaata caagttaagg tatctggtat ctgcatgtaa
     2461 aatcttcagc tggtggatgg tgacttttga agaacaaaag gctttggcaa cagaaaacaa
     2521 ttgttctggg tgatttctag aatggttttt gttgagtctc tgaacatcct aaatattggt
     2581 ttgttattct tttccagaaa gaaaatgaat ttgactggtt cacctgtgta ctgagtattg
     2641 ataaactttg aattttttta attgccttca attgggagag aaagctttat atttgtaaga
     2701 aatatatttg ataaagtttc ttaaagcaac accaaaaaaa caaaagaaaa gctaagtgaa
     2761 tttttgcaca ttctacacac agtgcctgta aatctcattt gtattttcag tttgccctta
     2821 attttttttg ttagtgttta gaaaacaatg ttttaaacat tcttcagtgt tctgatttct
     2881 tattaccccc tttcctcttg ggcttttgaa ctgtatttga tgttgctttg ggataatgtt
     2941 tataagtcaa acataagata ttgtacattg ggcacatatc tcctcttggg ctgctaataa
     3001 taaattaata acaggtaacc tggacaaacc aggaagcacc aaaccccttt tcagtttgaa
     3061 ctcttctttg ccaggtgtga ggacttctgc atcttacagt cagcacagaa cacactgaga
     3121 cttgaatcaa gtcagcaaca gagcaaaata aaggttagat aagtccttgt gtagcaaatt
     3181 tcgagcataa gaaataaaat ctaattaatt cttagggtac tcatctgact tgaactctgt
     3241 tggtttactg tgttagtaaa ctgtgctttc tattatctat acataaaacc tgagcagcaa
     3301 ctgtgtcttt agagctattg ccacattagc ctttgcactg tatagcgtct ggctttatgg
     3361 aacttaagtt taccaaatat aaaaagaaac ttctgctttt aaaaaaatta tatatatata
     3421 ttaaatttga aacctgcatt tctcccacag caatgtaaga agtaggctct gatgtcctac
     3481 cactttgaat ggttttctaa tatcttaatg aatagttcct gaacattgca ctgatatcat
     3541 cgattagaat tttgatattt aatttcatct ttatttcctg gtagagaatg caggaaaaga
     3601 tgtcaggtac ataacataaa acagattggg aatttattgt ttccaaaggg catggccttc
     3661 cttagcatca gtttgaagct tttgttatga cttagctgac ttgtggcagc ggggcaagca
     3721 aaaacaataa cactgcttat aaatggcacc acatcttgtt aacctccccc ccaaatactc
     3781 tctgaaagtc atgcacatac ctatgggatt ttacacacca ccagcttaaa atgctatgtc
     3841 tctatccatc agaaatagtc attattctat ttttaaggca gcaacaagaa aagaaaaaac
     3901 acttttcctg agggatttct aaccatgtat ctaatcctcc catttgggca gtataggtgt
     3961 ttgctttttt gttttctttt tttaagaaaa accttgaaac ctttgacact gacagatgtg
     4021 tttgcaagga tacggcttca gtattactaa tttccatgtg tatctggaag tatttttaaa
     4081 tggcatacca aaatccagaa gtttaaagat gcctataaaa gtaaacaaca tttatttaaa
     4141 aagaactctg aatatgcctt cttttttaat tagaaatatc ttcgagactt gggtgtttgt
     4201 taataactaa taactggagt aagctacagg atctaaagca gcccttttta cagtctagtt
     4261 aggagagaga aaataattgc aaatatccac ttagaggcaa agaacaattt tttattatca
     4321 aaaaggtttc tgcacattgt tgtggcaata ttgtatctgt ttagaaaatg ggcttttcca
     4381 aaagcaaaca aagataggtt cctcaggtga ccaaaactga aaatcaatat ttccatgttt
     4441 cattaatcaa ggcataaaat acaattaaag caaaatattt tacattaaaa
//
