LOCUS       NM_020356               4195 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens Cas scaffold protein family member 4 (CASS4),
            transcript variant 2, mRNA.
ACCESSION   NM_020356
VERSION     NM_020356.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Sugis,E., Dauvillier,J., Leontjeva,A., Adler,P., Hindie,V.,
            Moncion,T., Collura,V., Daudin,R., Loe-Mie,Y., Herault,Y.,
            Lambert,J.C., Hermjakob,H., Pupko,T., Rain,J.C., Xenarios,I.,
            Vilo,J., Simonneau,M. and Peterson,H.
  TITLE     HENA, heterogeneous network-based data set for Alzheimer's disease
  JOURNAL   Sci Data 6 (1), 151 (2019)
   PUBMED   31413325
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4195)
  AUTHORS   Hassan,M., Shahzadi,S., Alashwal,H., Zaki,N., Seo,S.Y. and
            Moustafa,A.A.
  TITLE     Exploring the mechanistic insights of Cas scaffolding protein
            family member 4 with protein tyrosine kinase 2 in Alzheimer's
            disease by evaluating protein interactions through molecular
            docking and dynamic simulations
  JOURNAL   Neurol Sci 39 (8), 1361-1374 (2018)
   PUBMED   29789968
  REMARK    GeneRIF: The results of this study showed that Cas scaffolding
            protein family member 4 with protein tyrosine kinase 2 and their
            significant role in the activation of downstream signaling pathways
            in Alzheimer's disease.
REFERENCE   4  (bases 1 to 4195)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 4195)
  AUTHORS   Li,A., Zhang,W., Xia,H., Miao,Y., Zhou,H., Zhang,X., Dong,Q.,
            Li,Q., Qiu,X. and Wang,E.
  TITLE     Overexpression of CASS4 promotes invasion in non-small cell lung
            cancer by activating the AKT signaling pathway and inhibiting
            E-cadherin expression
  JOURNAL   Tumour Biol 37 (11), 15157-15164 (2016)
   PUBMED   27677288
  REMARK    GeneRIF: High expression of CASS4 is associated with invasion in
            non-small cell lung cancer.
REFERENCE   6  (bases 1 to 4195)
  AUTHORS   Nikonova,A.S., Gaponova,A.V., Kudinov,A.E. and Golemis,E.A.
  TITLE     CAS proteins in health and disease: an update
  JOURNAL   IUBMB Life 66 (6), 387-395 (2014)
   PUBMED   24962474
  REMARK    Review article
REFERENCE   7  (bases 1 to 4195)
  AUTHORS   Lambert,J.C., Ibrahim-Verbaas,C.A., Harold,D., Naj,A.C., Sims,R.,
            Bellenguez,C., DeStafano,A.L., Bis,J.C., Beecham,G.W.,
            Grenier-Boley,B., Russo,G., Thorton-Wells,T.A., Jones,N.,
            Smith,A.V., Chouraki,V., Thomas,C., Ikram,M.A., Zelenika,D.,
            Vardarajan,B.N., Kamatani,Y., Lin,C.F., Gerrish,A., Schmidt,H.,
            Kunkle,B., Dunstan,M.L., Ruiz,A., Bihoreau,M.T., Choi,S.H.,
            Reitz,C., Pasquier,F., Cruchaga,C., Craig,D., Amin,N., Berr,C.,
            Lopez,O.L., De Jager,P.L., Deramecourt,V., Johnston,J.A., Evans,D.,
            Lovestone,S., Letenneur,L., Moron,F.J., Rubinsztein,D.C.,
            Eiriksdottir,G., Sleegers,K., Goate,A.M., Fievet,N.,
            Huentelman,M.W., Gill,M., Brown,K., Kamboh,M.I., Keller,L.,
            Barberger-Gateau,P., McGuiness,B., Larson,E.B., Green,R.,
            Myers,A.J., Dufouil,C., Todd,S., Wallon,D., Love,S., Rogaeva,E.,
            Gallacher,J., St George-Hyslop,P., Clarimon,J., Lleo,A., Bayer,A.,
            Tsuang,D.W., Yu,L., Tsolaki,M., Bossu,P., Spalletta,G., Proitsi,P.,
            Collinge,J., Sorbi,S., Sanchez-Garcia,F., Fox,N.C., Hardy,J., Deniz
            Naranjo,M.C., Bosco,P., Clarke,R., Brayne,C., Galimberti,D.,
            Mancuso,M., Matthews,F., Moebus,S., Mecocci,P., Del Zompo,M.,
            Maier,W., Hampel,H., Pilotto,A., Bullido,M., Panza,F., Caffarra,P.,
            Nacmias,B., Gilbert,J.R., Mayhaus,M., Lannefelt,L., Hakonarson,H.,
            Pichler,S., Carrasquillo,M.M., Ingelsson,M., Beekly,D., Alvarez,V.,
            Zou,F., Valladares,O., Younkin,S.G., Coto,E., Hamilton-Nelson,K.L.,
            Gu,W., Razquin,C., Pastor,P., Mateo,I., Owen,M.J., Faber,K.M.,
            Jonsson,P.V., Combarros,O., O'Donovan,M.C., Cantwell,L.B.,
            Soininen,H., Blacker,D., Mead,S., Mosley,T.H. Jr., Bennett,D.A.,
            Harris,T.B., Fratiglioni,L., Holmes,C., de Bruijn,R.F.,
            Passmore,P., Montine,T.J., Bettens,K., Rotter,J.I., Brice,A.,
            Morgan,K., Foroud,T.M., Kukull,W.A., Hannequin,D., Powell,J.F.,
            Nalls,M.A., Ritchie,K., Lunetta,K.L., Kauwe,J.S., Boerwinkle,E.,
            Riemenschneider,M., Boada,M., Hiltuenen,M., Martin,E.R.,
            Schmidt,R., Rujescu,D., Wang,L.S., Dartigues,J.F., Mayeux,R.,
            Tzourio,C., Hofman,A., Nothen,M.M., Graff,C., Psaty,B.M., Jones,L.,
            Haines,J.L., Holmans,P.A., Lathrop,M., Pericak-Vance,M.A.,
            Launer,L.J., Farrer,L.A., van Duijn,C.M., Van Broeckhoven,C.,
            Moskvina,V., Seshadri,S., Williams,J., Schellenberg,G.D. and
            Amouyel,P.
  CONSRTM   European Alzheimer's Disease Initiative (EADI); Genetic and
            Environmental Risk in Alzheimer's Disease; Alzheimer's Disease
            Genetic Consortium; Cohorts for Heart and Aging Research in Genomic
            Epidemiology
  TITLE     Meta-analysis of 74,046 individuals identifies 11 new
            susceptibility loci for Alzheimer's disease
  JOURNAL   Nat Genet 45 (12), 1452-1458 (2013)
   PUBMED   24162737
REFERENCE   8  (bases 1 to 4195)
  AUTHORS   Miao,Y., Wang,L., Liu,Y., Li,A.L., Liu,S.L., Cao,H.Y., Zhang,X.P.,
            Jiang,G.Y., Liu,D. and Wang,E.H.
  TITLE     Overexpression and cytoplasmic accumulation of Hepl is associated
            with clinicopathological parameters and poor prognosis in non-small
            cell lung cancer
  JOURNAL   Tumour Biol 34 (1), 107-114 (2013)
   PUBMED   23001926
  REMARK    GeneRIF: Hepl is overexpressed in the nucleus and aberrantly
            accumulates in the cytoplasm of non-small cell lung cancer cells,
            and Hepl may play a role in the progression of lung cancer,
            including lymph node metastasis and TNM stage.
REFERENCE   9  (bases 1 to 4195)
  AUTHORS   Singh,M.K., Dadke,D., Nicolas,E., Serebriiskii,I.G., Apostolou,S.,
            Canutescu,A., Egleston,B.L. and Golemis,E.A.
  TITLE     A novel Cas family member, HEPL, regulates FAK and cell spreading
  JOURNAL   Mol Biol Cell 19 (4), 1627-1636 (2008)
   PUBMED   18256281
  REMARK    GeneRIF: HEPL maintains Cas family function in localization to
            focal adhesions, as well as regulation of FAK activity, focal
            adhesion integrity, and cell spreading.
REFERENCE   10 (bases 1 to 4195)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA584687.1, AK314653.1, AK295613.1, AK027760.1 and AL121914.31.
            
            On Jun 2, 2019 this sequence version replaced NM_020356.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.141160.1,
                                           SRR1803616.182557.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000679887.1/ ENSP00000506506.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                DA584687.1         4-68
            66-1703             AK314653.1         1-1638
            1704-2817           AK295613.1         1409-2522
            2818-3153           AK027760.1         1506-1841
            3154-4195           AL121914.31        131347-132388
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.31"
     gene            1..4195
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /note="Cas scaffold protein family member 4"
                     /db_xref="GeneID:57091"
                     /db_xref="HGNC:HGNC:15878"
                     /db_xref="MIM:618888"
     exon            1..235
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..2560
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /note="isoform a is encoded by transcript variant 2;
                     HEF-like protein; HEF1-EFS-p130Cas-like protein; Cas
                     scaffolding protein family member 4"
                     /codon_start=1
                     /product="cas scaffolding protein family member 4 isoform
                     a"
                     /protein_id="NP_065089.2"
                     /db_xref="CCDS:CCDS33492.1"
                     /db_xref="GeneID:57091"
                     /db_xref="HGNC:HGNC:15878"
                     /db_xref="MIM:618888"
                     /translation="MKGTGIMDCAPKALLARALYDNCPDCSDELAFSRGDILTILEQH
                     VPESEGWWKCLLHGRQGLAPANRLQILTEVAADRPCPPFLRGLEEAPASSEETYQVPT
                     LPRPPTPGPVYEQMRSWAEGPQPPTAQVYEFPDPPTSARIICEKTLSFPKQAILTLPR
                     PVRASLPTLPSQVYDVPTQHRGPVVLKEPEKQQLYDIPASPKKAGLHPPDSQASGQGV
                     PLISVTTLRRGGYSTLPNPQKSEWIYDTPVSPGKASVRNTPLTSFAEESRPHALPSSS
                     STFYNPPSGRSRSLTPQLNNNVPMQKKLSLPEIPSYGFLVPRGTFPLDEDVSYKVPSS
                     FLIPRVEQQNTKPNIYDIPKATSSVSQAGKELEKAKEVSENSAGHNSSWFSRRTTSPS
                     PEPDRLSGSSSDSRASIVSSCSTTSTDDSSSSSSEESAKELSLDLDVAKETVMALQHK
                     VVSSVAGLMLFVSRKWRFRDYLEANIDAIHRSTDHIEESVREFLDFARGVHGTACNLT
                     DSNLQNRIRDQMQTISNSYRILLETKESLDNRNWPLEVLVTDSVQNSPDDLERFVMVA
                     RMLPEDIKRFASIVIANGRLLFKRNCEKEETVQLTPNAEFKCEKYIQPPQRETESHQK
                     STPSTKQREDEHSSELLKKNRANICGQNPGPLIPQPSSQQTPERKPRLSEHCRLYFGA
                     LFKAISAFHGSLSSSQPAEIITQSKLVIMVGQKLVDTLCMETQERDVRNEILRGSSHL
                     CSLLKDVALATKNAVLTYPSPAALGHLQAEAEKLEQHTRQFRGTLG"
     exon            236..658
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /inference="alignment:Splign:2.1.0"
     exon            659..760
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /inference="alignment:Splign:2.1.0"
     exon            761..841
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /inference="alignment:Splign:2.1.0"
     exon            842..2152
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..4195
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4176..4181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /note="hexamer: AATATA"
     polyA_site      4195
                     /gene="CASS4"
                     /gene_synonym="C20orf32; CAS4; HEFL; HEPL"
                     /note="major polyA site"
ORIGIN      
        1 cattttactc ttatcgtgct ttccagaaag tttgcctgct gggagagtct ttttgatcgt
       61 ttcccatgtg ttgtcagata gctccataga attcagtttc tgagaaccag ccagaagcat
      121 gcagtgacat tgcacaatct gcctctgaag ctggagatac tagctgcaga gctcagggga
      181 gctgctccac atcaccgaca tgaagggaac aggcatcatg gactgtgcgc ccaaggcact
      241 cctggccagg gcactttatg acaactgccc tgactgctct gacgagctgg ctttcagcag
      301 aggggacatc ctgaccattc tggagcaaca cgtgccagaa agcgagggtt ggtggaagtg
      361 tttgctccat gggaggcaag gcctggcccc tgccaaccgc ctccaaatcc tcacggaggt
      421 cgctgcagac aggccgtgcc ccccattcct gagaggcctg gaagaagctc ctgccagctc
      481 agaggagacc tatcaggtgc ccactctacc ccgccctccc actccaggcc ccgtttatga
      541 gcagatgagg agttgggcgg aggggcccca gccccctact gcccaagtct atgaattccc
      601 cgaccctccc accagtgcca gaatcatctg tgaaaagact ctcagctttc caaaacaggc
      661 catcctcacg cttcccagac ctgtccgggc ctcactgccg actctgcctt cccaggtgta
      721 tgacgtgcct acccagcacc ggggccccgt ggtcctgaag gagccagaga agcagcagtt
      781 atatgacata ccagccagcc ccaagaaggc aggactccat cccccagaca gccaagcaag
      841 tgggcagggt gttcccctga tatcagtgac taccttaaga agaggcggtt acagcacatt
      901 accaaatcct cagaaatcgg aatggattta tgacactcca gtgtctccag gaaaggccag
      961 cgtcagaaac acgcctctca ccagctttgc ggaagaatca aggccccacg ctctccccag
     1021 ttccagctcc actttctaca atcctccaag tggcagatcc aggtccctca ctccacaact
     1081 gaataacaat gtgcccatgc agaaaaaact cagccttcca gaaattcctt cttatggctt
     1141 tcttgtaccc agaggcacat ttcctttgga tgaagatgtc agctacaagg ttccttcaag
     1201 ctttctgatt ccccgagtgg aacagcagaa caccaagccc aatatttatg acatccctaa
     1261 agcaacgtcg agtgtttctc aggctgggaa ggagctggag aaagccaagg aggtgtcaga
     1321 gaattccgcg ggccataatt cctcatggtt ctccagacgg acaacttccc catctcctga
     1381 accggacaga ttatcaggtt ccagttctga cagcagagct agcatcgttt cctcgtgctc
     1441 caccacatcc accgacgact cctccagctc ttcctcggag gagtcagcaa aggagctctc
     1501 cttggacctg gatgtggcca aggagacagt gatggctctg cagcacaagg tggtcagctc
     1561 tgtcgctggc ctgatgctct ttgtcagcag gaagtggaga ttccgagact atctggaggc
     1621 caacattgat gcaatccaca ggtccactga tcacatagaa gaatctgtaa gagaatttct
     1681 ggattttgcc cgaggagtcc atgggactgc ctgtaacctc actgacagta accttcagaa
     1741 cagaattcgg gaccagatgc agaccatctc caactcctac cgcatcctgc ttgaaacaaa
     1801 ggaaagcttg gataatcgca attggcctct ggaagttctt gtgactgaca gtgtccagaa
     1861 cagcccagat gaccttgaga ggtttgtcat ggtggcacgg atgcttccag aagacatcaa
     1921 gaggtttgcc tccattgtca ttgccaatgg aaggctcctt tttaagcgga actgtgaaaa
     1981 ggaagagact gtgcagttga ccccaaatgc agaatttaag tgtgaaaaat acatccagcc
     2041 tccccaaaga gaaactgaat cacaccaaaa gagtacccct tccactaagc aaagggaaga
     2101 tgaacactct tctgaactat taaagaaaaa tagggcaaat atctgtggac agaatcctgg
     2161 ccctcttata cctcagcctt cgagtcaaca gactcctgag aggaaacccc gcttatctga
     2221 acactgccgg ctctactttg gggcgctctt caaagccatc agcgcatttc acggcagcct
     2281 cagcagcagc cagcccgcgg agatcatcac tcagagcaag ctggtcatca tggtgggaca
     2341 gaagctggtg gacacgctgt gcatggagac ccaggagagg gacgtgcgca acgagatcct
     2401 ccgtggcagc agtcacctct gcagcctgct caaggacgta gcgctggcca ctaagaatgc
     2461 cgtgctcacg taccccagcc ctgccgcgct ggggcacctc caggcggagg ctgagaagct
     2521 ggagcaacac acgcggcagt tcagagggac actgggatga ggactgtcta cctcccttcc
     2581 tcctctgctc acctctcagc ttcacaccca caacttgtgc aactctgccc tatgggaaaa
     2641 gccagccggg gcatacacca atgagctgaa acagacctgg tgcccaaagc tggtagtacc
     2701 aagtggctaa gcaaccccag ggcattgact taccccgcag ggggtcagga aagagagtca
     2761 ggtatgaggt aaagaccttg tgaagtttag catatatgga gtatgcagta tcagcttgaa
     2821 gtgacttcgc agaaataata ttttatattg attaaatgtg tgcatgtttt gtttgttttt
     2881 tgagacaaag tctcactctg tcacacaggc tggagtgcag tggcgcgatc tcagctcact
     2941 gcaacctccg ccttctgggt tgaagcaatt ctcccgcctc agcctccaga gtagctggta
     3001 ctacaggtgt gcgccaccat gcccagctaa tttttgtatt tttttttaat gaaaggtctc
     3061 acatatttat tactgaaccc agccaaccaa tgcattcata acagattcag aaagaaaaaa
     3121 tatattccca ataaaacatg tacaattctc cagagagcgg tgacattttc agcttgatgt
     3181 ggtaacatga ctgtgacctt cagacagcat aaatatgtgt gccctctcat gtgcaattcc
     3241 ttatagggct agcttggttc ttcttcaatg tctccttttg gagttgtacc tgattttatt
     3301 accagttttc atctgaatcc actggggagt ggggcgattt tgttttaatt tctcggtcag
     3361 gaatcactta atcctggaag tcttatgaga agacatggca agaagcagag tcaagcacac
     3421 accacaatgg cagagaaagg aagagctaat ttttgtattt ttagtagagt cggggtttca
     3481 ccatattagc caggctggtc tggaactcct gacctcaggc catcccccgc ctcggcctcc
     3541 caaagtgctg ggattacagg cctgagccac cattcctggc atgtacgtgt tttaaattca
     3601 ctcatttaac acttatgtat tggatgccta ctacatgcca agttctaggt gctggagaca
     3661 tagcagtgtt tcagagggct tgccctcttg gaacttatga tctagtaggg gagtcaggca
     3721 gtaaagcggt gaagaaatac atacacagta tagcaggtgc caggaaaaac gtaagtaggg
     3781 gaagtgcaag agaacttatc ttcaaatggt taatttttat tgcatccatt agataataga
     3841 gtaagttttt tgttgttttg aattgaatta gttctgtgag attggtacaa attatgtatg
     3901 tgtcttgcac ccataaatat atatttttgc tatgttactt taacctaaag atagatgtta
     3961 tatttacaac ataacatgat ctgttgggtt ctctattaat ttatttcata cattatagat
     4021 tctgggttga taaattctgc tcaatttgaa aaattttata ttttgtctca aatctgttac
     4081 aatttatatt ggttggtgca aaagtaattg cggtttttgc cgtagacagt aatggcaaaa
     4141 accacaattg cttttgcacc accctaataa tattaaatat aatcatgact ttaaa
//
