LOCUS       NM_000388              10062 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens calcium sensing receptor (CASR), transcript variant 2,
            mRNA.
ACCESSION   NM_000388
VERSION     NM_000388.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Li,J., Ke,K., Zhang,B., Liu,Y., Bai,J., Wang,M. and Li,H.
  TITLE     Association of single nucleotide genetic polymorphisms of vitamin D
            receptor and calcium-sensitive receptor with calcium-containing
            kidney stones in Chinese Dai populations: a prospective
            multi-center study
  JOURNAL   Int Urol Nephrol 56 (11), 3647-3655 (2024)
   PUBMED   38886300
  REMARK    GeneRIF: Association of single nucleotide genetic polymorphisms of
            vitamin D receptor and calcium-sensitive receptor with
            calcium-containing kidney stones in Chinese Dai populations: a
            prospective multi-center study.
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Adam,S., Maas,S.L., Huchzermeier,R., Rakateli,L., Abschlag,K.,
            Hohl,M., Liao,L., Bartneck,M., Teunissen,M., Wouters,K.,
            Santovito,D., Jankowski,J., Biessen,E.A.L. and van der Vorst,E.P.C.
  TITLE     The calcium-sensing-receptor (CaSR) in adipocytes contributes to
            sex-differences in the susceptibility to high fat diet induced
            obesity and atherosclerosis
  JOURNAL   EBioMedicine 107, 105293 (2024)
   PUBMED   39146692
  REMARK    GeneRIF: The calcium-sensing-receptor (CaSR) in adipocytes
            contributes to sex-differences in the susceptibility to high fat
            diet induced obesity and atherosclerosis.
REFERENCE   3  (bases 1 to 10062)
  AUTHORS   Zanchelli,F., Giudicissi,A., Neri,L., Sgarlato,V., Bruno,P.F.,
            Ruggeri,M., Signorotti,S., Apuzzo,D., Notaro,E. and Buscaroli,A.
  TITLE     [Familial Hypocalciuric Hypercalcemia Type 1 Likely Secondary to a
            New Inactivating Mutation of CASR]
  JOURNAL   G Ital Nefrol 41 (4) (2024)
   PUBMED   39243411
  REMARK    GeneRIF: [Familial Hypocalciuric Hypercalcemia Type 1 Likely
            Secondary to a New Inactivating Mutation of CASR].
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10062)
  AUTHORS   Markowska,A., de Mezer,M., Kurzawa,P., Bednarek,W., Grybos,A.,
            Krzyzaniak,M., Markowska,J., Grybos,M. and Zurawski,J.
  TITLE     Analysis of Expression of the ANG1, CaSR and FAK Proteins in
            Uterine Fibroids
  JOURNAL   Int J Mol Sci 25 (13), 7164 (2024)
   PUBMED   39000274
  REMARK    GeneRIF: Analysis of Expression of the ANG1, CaSR and FAK Proteins
            in Uterine Fibroids.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10062)
  AUTHORS   Xie,W., Xu,H., Cheng,Y., Lin,X., Zeng,J. and Sun,Y.
  TITLE     Calcium-sensing Receptor, a Potential Biomarker Revealed by
            Large-scale Public Databases and Experimental Verification in
            Metastatic Breast Cancer
  JOURNAL   Technol Cancer Res Treat 23, 15330338241254219 (2024)
   PUBMED   38780484
  REMARK    GeneRIF: Calcium-sensing Receptor, a Potential Biomarker Revealed
            by Large-scale Public Databases and Experimental Verification in
            Metastatic Breast Cancer.
REFERENCE   6  (bases 1 to 10062)
  AUTHORS   Aida,K., Koishi,S., Tawata,M. and Onaya,T.
  TITLE     Molecular cloning of a putative Ca(2+)-sensing receptor cDNA from
            human kidney
  JOURNAL   Biochem Biophys Res Commun 214 (2), 524-529 (1995)
   PUBMED   7677761
REFERENCE   7  (bases 1 to 10062)
  AUTHORS   Aida,K., Koishi,S., Inoue,M., Nakazato,M., Tawata,M. and Onaya,T.
  TITLE     Familial hypocalciuric hypercalcemia associated with mutation in
            the human Ca(2+)-sensing receptor gene
  JOURNAL   J Clin Endocrinol Metab 80 (9), 2594-2598 (1995)
   PUBMED   7673400
REFERENCE   8  (bases 1 to 10062)
  AUTHORS   Garrett,J.E., Capuano,I.V., Hammerland,L.G., Hung,B.C., Brown,E.M.,
            Hebert,S.C., Nemeth,E.F. and Fuller,F.
  TITLE     Molecular cloning and functional expression of human parathyroid
            calcium receptor cDNAs
  JOURNAL   J Biol Chem 270 (21), 12919-12925 (1995)
   PUBMED   7759551
REFERENCE   9  (bases 1 to 10062)
  AUTHORS   Chou,Y.H., Pollak,M.R., Brandi,M.L., Toss,G., Arnqvist,H.,
            Atkinson,A.B., Papapoulos,S.E., Marx,S., Brown,E.M., Seidman,J.G.
            et al.
  TITLE     Mutations in the human Ca(2+)-sensing-receptor gene that cause
            familial hypocalciuric hypercalcemia
  JOURNAL   Am J Hum Genet 56 (5), 1075-1079 (1995)
   PUBMED   7726161
REFERENCE   10 (bases 1 to 10062)
  AUTHORS   Shelton,C., LaRusch,J. and Whitcomb,D.C.
  TITLE     Pancreatitis Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24624459
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068630.21, AC068754.16 and
            AC083798.14.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_000388.3.
            
            Summary: The protein encoded by this gene is a plasma membrane G
            protein-coupled receptor that senses small changes in circulating
            calcium concentration. The encoded protein couples this information
            to intracellular signaling pathways that modify parathyroid hormone
            secretion or renal cation handling, and thus this protein plays an
            essential role in maintaining mineral ion homeostasis. Mutations in
            this gene are a cause of familial hypocalciuric hypercalcemia,
            neonatal severe hyperparathyroidism, and autosomal dominant
            hypocalcemia. [provided by RefSeq, Aug 2017].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: U20759.1, BC112236.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA1970526, SAMEA2142586 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000639785.2/ ENSP00000491584.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AC068630.21        22126-22270         c
            146-572             AC068754.16        50010-50436
            573-879             AC068754.16        53143-53449
            880-1764            AC068754.16        57590-58474
            1765-1995           AC068754.16        71874-72104
            1996-2119           AC068754.16        78175-78298
            2120-5619           AC068754.16        79749-83248
            5620-10062          AC083798.14        2006-6448
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.33-q21.1"
     gene            1..10062
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="calcium sensing receptor"
                     /db_xref="GeneID:846"
                     /db_xref="HGNC:HGNC:1514"
                     /db_xref="MIM:601199"
     exon            1..145
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /inference="alignment:Splign:2.1.0"
     exon            146..572
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             388..3624
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; parathyroid Ca(2+)-sensing receptor 1;
                     extracellular calcium-sensing receptor; parathyroid cell
                     calcium-sensing receptor 1"
                     /codon_start=1
                     /product="extracellular calcium-sensing receptor isoform 2
                     precursor"
                     /protein_id="NP_000379.3"
                     /db_xref="CCDS:CCDS3010.1"
                     /db_xref="GeneID:846"
                     /db_xref="HGNC:HGNC:1514"
                     /db_xref="MIM:601199"
                     /translation="MAFYSCCWVLLALTWHTSAYGPDQRAQKKGDIILGGLFPIHFGV
                     AAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCN
                     TVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLF
                     YIPQVSYASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDY
                     GRPGIEKFREEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDL
                     EPLIKEIVRRNITGKIWLASEAWASSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFL
                     KKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFLRGHEESGDRFSNSSTAFR
                     PLCTGDENISSVETPYIDYTHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCA
                     DIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEV
                     GYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCLAGTRKGIIEGEPTCCFECV
                     ECPDGEYSDETDASACNKCPDDFWSNENHTSCIAKEIEFLSWTEPFGIALTLFAVLGI
                     FLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQP
                     AFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQIVICV
                     IWLYTAPPSSYRNQELEDEIIFITCHEGSLMALGFLIGYTCLLAAICFFFAFKSRKLP
                     ENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNK
                     IYIILFKPSRNTIEEVRCSTAAHAFKVAARATLRRSNVSRKRSSSLGGSTGSTPSSSI
                     SSKSNSEDPFPQPERQKQQQPLALTQQEQQQQPLTLPQQQRSQQQPRCKQKVIFGSGT
                     VTFSLSFDEPQKNAMAHRNSTHQNSLEAQKSSDTLTRHEPLLPLQCGETDLDLTVQET
                     GLQGPVGGDQRPEVEDPEELSPALVVSSSQSFVISGGGSTVTENVVNS"
     exon            573..879
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1764
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1995
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2119
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..10062
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4369..4374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="hexamer: TATAAA"
     polyA_site      4387
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
     regulatory      4907..4912
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="hexamer: AATAAA"
     polyA_site      4927
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="major polyA site"
     regulatory      5241..5246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="hexamer: ATTAAA"
     polyA_site      5263
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
     regulatory      7178..7183
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="hexamer: AATAAA"
     polyA_site      7200
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
     regulatory      8351..8356
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="hexamer: AATAAA"
     polyA_site      8367
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
     regulatory      8923..8928
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
                     /note="hexamer: AATATA"
     polyA_site      8945
                     /gene="CASR"
                     /gene_synonym="CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC;
                     HHC1; HYPOC1; NSHPT; PCAR1"
ORIGIN      
        1 agagtagacc aagtgcaaca ggcacctggc tgcagccagg aaggaccgca cgccctttcg
       61 cgcaggagag tggaaggagg gagctgtttg ccagcaccga ggtcttgcgg cacaggcaac
      121 gcttgacctg agtcttgcag aatgaaaggc atcacaggag gcctctgcat gatgtggctt
      181 ccaaagactc aaggaccacc cacattacaa gtctggattg aggaaggcag aaatggagat
      241 tcaaacacca cgtcttctat tattttatta atcaatctgt agacatgtgt ccccactgca
      301 gggagtgaac tgctccaagg gagaaacttc tgggagcctc caaactccta gctgtctcat
      361 cccttgccct ggagagacgg cagaaccatg gcattttata gctgctgctg ggtcctcttg
      421 gcactcacct ggcacacctc tgcctacggg ccagaccagc gagcccaaaa gaagggggac
      481 attatccttg gggggctctt tcctattcat tttggagtag cagctaaaga tcaagatctc
      541 aaatcaaggc cggagtctgt ggaatgtatc aggtataatt tccgtgggtt tcgctggtta
      601 caggctatga tatttgccat agaggagata aacagcagcc cagcccttct tcccaacttg
      661 acgctgggat acaggatatt tgacacttgc aacaccgttt ctaaggcctt ggaagccacc
      721 ctgagttttg ttgctcaaaa caaaattgat tctttgaacc ttgatgagtt ctgcaactgc
      781 tcagagcaca ttccctctac gattgctgtg gtgggagcaa ctggctcagg cgtctccacg
      841 gcagtggcaa atctgctggg gctcttctac attccccagg tcagttatgc ctcctccagc
      901 agactcctca gcaacaagaa tcaattcaag tctttcctcc gaaccatccc caatgatgag
      961 caccaggcca ctgccatggc agacatcatc gagtatttcc gctggaactg ggtgggcaca
     1021 attgcagctg atgacgacta tgggcggccg gggattgaga aattccgaga ggaagctgag
     1081 gaaagggata tctgcatcga cttcagtgaa ctcatctccc agtactctga tgaggaagag
     1141 atccagcatg tggtagaggt gattcaaaat tccacggcca aagtcatcgt ggttttctcc
     1201 agtggcccag atcttgagcc cctcatcaag gagattgtcc ggcgcaatat cacgggcaag
     1261 atctggctgg ccagcgaggc ctgggccagc tcctccctga tcgccatgcc tcagtacttc
     1321 cacgtggttg gcggcaccat tggattcgct ctgaaggctg ggcagatccc aggcttccgg
     1381 gaattcctga agaaggtcca tcccaggaag tctgtccaca atggttttgc caaggagttt
     1441 tgggaagaaa catttaactg ccacctccaa gaaggtgcaa aaggaccttt acctgtggac
     1501 acctttctga gaggtcacga agaaagtggc gacaggttta gcaacagctc gacagccttc
     1561 cgacccctct gtacagggga tgagaacatc agcagtgtcg agacccctta catagattac
     1621 acgcatttac ggatatccta caatgtgtac ttagcagtct actccattgc ccacgccttg
     1681 caagatatat atacctgctt acctgggaga gggctcttca ccaatggctc ctgtgcagac
     1741 atcaagaaag ttgaggcgtg gcaggtcctg aagcacctac ggcatctaaa ctttacaaac
     1801 aatatggggg agcaggtgac ctttgatgag tgtggtgacc tggtggggaa ctattccatc
     1861 atcaactggc acctctcccc agaggatggc tccatcgtgt ttaaggaagt cgggtattac
     1921 aacgtctatg ccaagaaggg agaaagactc ttcatcaacg aggagaaaat cctgtggagt
     1981 gggttctcca gggaggtgcc cttctccaac tgcagccgag actgcctggc agggaccagg
     2041 aaagggatca ttgaggggga gcccacctgc tgctttgagt gtgtggagtg tcctgatggg
     2101 gagtatagtg atgagacaga tgccagtgcc tgtaacaagt gcccagatga cttctggtcc
     2161 aatgagaacc acacctcctg cattgccaag gagatcgagt ttctgtcgtg gacggagccc
     2221 tttgggatcg cactcaccct ctttgccgtg ctgggcattt tcctgacagc ctttgtgctg
     2281 ggtgtgttta tcaagttccg caacacaccc attgtcaagg ccaccaaccg agagctctcc
     2341 tacctcctcc tcttctccct gctctgctgc ttctccagct ccctgttctt catcggggag
     2401 ccccaggact ggacgtgccg cctgcgccag ccggcctttg gcatcagctt cgtgctctgc
     2461 atctcatgca tcctggtgaa aaccaaccgt gtcctcctgg tgtttgaggc caagatcccc
     2521 accagcttcc accgcaagtg gtgggggctc aacctgcagt tcctgctggt tttcctctgc
     2581 accttcatgc agattgtcat ctgtgtgatc tggctctaca ccgcgccccc gtcaagctac
     2641 cgcaaccagg agctggagga tgagatcatc ttcatcacgt gccacgaggg ctccctcatg
     2701 gccctgggct tcctgatcgg ctacacctgc ctgctggctg ccatctgctt cttctttgcc
     2761 ttcaagtccc ggaagctgcc ggagaacttc aatgaagcca agttcatcac cttcagcatg
     2821 ctcatcttct tcatcgtctg gatctccttc attccagcct atgccagcac ctatggcaag
     2881 tttgtctctg ccgtagaggt gattgccatc ctggcagcca gctttggctt gctggcgtgc
     2941 atcttcttca acaagatcta catcattctc ttcaagccat cccgcaacac catcgaggag
     3001 gtgcgttgca gcaccgcagc tcacgctttc aaggtggctg cccgggccac gctgcgccgc
     3061 agcaacgtct cccgcaagcg gtccagcagc cttggaggct ccacgggatc caccccctcc
     3121 tcctccatca gcagcaagag caacagcgaa gacccattcc cacagcccga gaggcagaag
     3181 cagcagcagc cgctggccct aacccagcaa gagcagcagc agcagcccct gaccctccca
     3241 cagcagcaac gatctcagca gcagcccaga tgcaagcaga aggtcatctt tggcagcggc
     3301 acggtcacct tctcactgag ctttgatgag cctcagaaga acgccatggc ccacaggaat
     3361 tctacgcacc agaactccct ggaggcccag aaaagcagcg atacgctgac ccgacacgag
     3421 ccattactcc cgctgcagtg cggggaaacg gacttagatc tgaccgtcca ggaaacaggt
     3481 ctgcaaggac ctgtgggtgg agaccagcgg ccagaggtgg aggaccctga agagttgtcc
     3541 ccagcacttg tagtgtccag ttcacagagc tttgtcatca gtggtggagg cagcactgtt
     3601 acagaaaacg tagtgaattc ataaaatgga aggagaagac tgggctaggg agaatgcaga
     3661 gaggtttctt ggggtcccag ggaagaggaa tcgccccaga ctcctttcct ctgaggaaga
     3721 agggataata gacacatcaa atgccccgaa tttagtcaca ccatcttaaa tgacagtgaa
     3781 ttgacccatg ttccctttaa aattaaaaaa aagaagagcc ttgtgtttct gtggttgcat
     3841 ttgtcaaagc attgagatct ccacggtcag atttgctgtt cacccacatc taatgtctct
     3901 tcctctgttc tatcccaccc aacagctcag agatgaaact atggctttaa actaccctcc
     3961 agagtgtgca gactgatggg acatcaaatt tgccaccact agagctgaga gtctgaaaga
     4021 cagaatgtca ccagtcctgc ccaatgcctt gacaacagac tgaattttaa atgttcacaa
     4081 cataaggaga atgtatctcc tcctatttat gaaaaccata tgatattttg tctcctacct
     4141 gctgctgcta ttatgtaaca tccagaaggt ttgcacccct cctataccat atgtctgctt
     4201 ctgtccagga catgatactg atgccatgtt tagattccag gatcacaaga atcacctcaa
     4261 attgttagga agggactgca taaaccaatg agctgtatct gtaattaata ttcctatatg
     4321 tagctttatc cttaggaaaa tgcttctgtt gtaatagtcc atggacaata taaactgaaa
     4381 aatgtcagtc tggtttatat aaggcagtat tattgagctc tatttcccca ccccactatc
     4441 ctcactccca taagctaagc cttatgtgag ccccttcagg gactcaaggg tccagaagtc
     4501 cctcccatct ctaccccaaa gaattcctga agccagatcc accctatccc tgtacagagt
     4561 aagttctcaa ttattggcct gctaatagct gctagggtag gaaagcgtgg ttccaagaaa
     4621 gatccaccct caaatgtcag agctatgttc cctccagcag tggtattaat actgccggtc
     4681 acccaggctc tggagccaga gagacagacc ggggttcaag ccatggcttc gtcatttgca
     4741 agctgagtga ctgtaggcag ggaaccttaa cctctctaag ccacagcttc ttcatcttta
     4801 aaataaggat aataatcatt ctttcccctc agagctctta tgtggattaa acgagataat
     4861 gtatataaag tactttagcc tggtacctag cacacaataa gcattcaata aatattagtt
     4921 aatattatta ctgatactgt aataattatt ttttccctta cttcaaatac aaaacaaaac
     4981 atacatatta accattcaga ttgtactgcc tagcccactg atcacaatca ctctctctgt
     5041 aataagccca agagctctgg gcccaacaca tggggtcagc catagtatca ctggctcatg
     5101 gtgaggaagc cagaaagctg gtcagcccct caggtggccc aatgccctgt ctccgcagtg
     5161 tcagggcctg gacctccagc atccagggaa gaccatcacc agtctagcag gaggttctaa
     5221 gctgaggcca aaccattgca attaaatctc ctgaagcctc aaattttaca gctcttggtg
     5281 acttcttccc ctgaccactt tcttccaaag gggcgccttt cagtgatttg tagggcaaat
     5341 tggtggactt gatagttgtt aagattataa tgagaaatca ttagagggca ccatgtggga
     5401 gcgcgactgt gagcagaagg tggtgactca ggatgaccca agtgaggtgc ctgctcccca
     5461 cctaggaagg gcaccggccc ccacccagcc tgcgcccccc tgcagtcatg cctctgagca
     5521 aggcaccgag ctgagtgggg atttatattt aacattttac ttggtcttaa aagaaatgct
     5581 gaaaatgtga tggtttcaaa ataactatga gggaacagcc tcaacagaat gtgacatact
     5641 tctccctcca gggtttgact tcaatgtcca tgtcacccaa ggggctttag gaggaaaaca
     5701 taggtttaca gtgagctttt atttcaccaa gtgcttgcaa ataaggagtc tctcccaatg
     5761 ctgttgtcag aaaggcagat acagaaacac tgtcccaatt tatacgtagc aggcacaact
     5821 cagcacaaaa tcaggggtgt gaaatatgaa cttgcattgt ttttctacaa caacttaatt
     5881 tctatctcaa attcccctcc agcctagtga cagaaacttc atcttagtcg aatcggggtt
     5941 ttcacagtaa cctcagtgtt ttctgagcca atttagatgt ttggggatca attcagaggt
     6001 tgttccttct cccttccagg agggcttttg taatgttggg gtgggcctcc tcagtcatca
     6061 gcagctccct catgacattg ctgaaagcaa tcttatctgg ccttcgctgg cgtgctctga
     6121 ggcattgtat cctcatctgg tcccatcatg ggtttcgtgg tggttttcac gcatgcctca
     6181 ctgtcctaac cagtcggtcc ctgtggggcc tgctagctct tgcagctacc tccatgctca
     6241 tccctgaggc ccagaaatca ctggcagcct cactttcatg tctacttgac tgctgtctcc
     6301 ccatgacagt acacagagct tatggaggct aacaaagctt ccagggtacc agagaagaaa
     6361 cagggtacat gtctccaaac ctgaatagac ttcgtagtgg gcaaataatg gtcacccaaa
     6421 gatgcccaca tcctcatctc tagaacctat ggctatgttg ttacatggca agggtgaatt
     6481 aaagtatagt ggaattaaga ttgctaatca gctgaccttg agataggaag agcgtcccaa
     6541 attatccagg tgggtccagt ggaatcccaa gggcccttaa atgtggacga agcagaagag
     6601 tcagtgtgag agcaatacag tatgagaaaa acaccactgg cctgtactgc ctttgcgtag
     6661 ggaagtggac atgagctgag gaaggcaggc tgcttctaga agctggcaaa tgccagaaaa
     6721 ccgattctcc cctaaaacat ccagaaagga atgcagccct gccaacatct tgattttagc
     6781 ccagtgacac ccttgtagga ctttggacct ccagatctgt aagataatac atttgtgttg
     6841 ttttaagcca tgaagtttat agtaatttgt ttcagcagca acagaaaaca aatacatttg
     6901 tattgttatc tccttccctg agttttggca gccagagcct gtctcagaga tctattcctg
     6961 ttctcactgt ggtcctcttc ccttcaccta gctcactcct tcccctctaa tattctattc
     7021 ctctacatct gcttgggaaa gtataaacct catattctaa gtctttgctg gctttggtta
     7081 tagaaaatac acagccgtgt tgtctcattc tattatctgt cagtagcaga aacctgaaat
     7141 tggtagcttc aacaaactag tgtttgcttt tctcttgaat aaaggaagtc aggaggtaaa
     7201 tatatgatca ccttttaatt actttttaaa atatttctta ggttttgtta acaatgggaa
     7261 actgcttaca tttcttttga aaattaaaat gaattataaa gttaaaaaga ttttcactca
     7321 aatgaaagtg tcctagaaga ttttaagaat ttaggttgga aatttagagg tgacgatcag
     7381 catataacat caataccctg aaagtcccta tgatcgtcac acgttgtcta attgcattaa
     7441 ttttgatttt ggggaagttg gggctaccct acctctgtct ccaaaaggca catttcaagg
     7501 caatgttgtc tgtcgttttg ctctggctga aatcacatag ctacgggctg gttctgccca
     7561 gccagagctt ccctttgctc atctgctgca tttgaaataa ttattgctac aagaataatg
     7621 gaacatcaat aaggggtgct aatcaccgga ggaggtggcc ctcatttgtc tgagcctgag
     7681 tggtcatttt aggagtccac tggagacaag aatactccca aaggtgggtg gaattagggt
     7741 aggtgggagg aagttgccac agtgagatca cagcctctgg gagatgctgg ttttcctggc
     7801 ccagatcatg gaaaaaaaac atcaagactt ggatgacaac tttgagtgac tttgctatta
     7861 tgcaaggagt gaggcagggg tcagggaaac aacaagcaca gggtagaacc tgtcacagga
     7921 ataaaggtgc attccaagga tccaaagaag aaccttagcc ttgagaggaa aagctcagca
     7981 agtggtatga gaaatggaga actgctaggt gaaggccaac agggcaggaa gccgggcaga
     8041 gccagcatga aggtgcctct gggaactgga gggacaactg tgactctagc accgagtggg
     8101 gctaggggaa ccttctcctc tgttgggaat aaccaccaca ttcatcccct cccctgaccc
     8161 ctggacaaga gtggaggaca cttggagtgg agacaagcag gggtacctgt ttaaatcaac
     8221 agctgaggcc agatgcagag gctcatgcct gcaatcccag cactttggga ggctgaggtg
     8281 ggaggatcac ttgagcccta agagttgaag accagcctgg gcaacatggc aaaaccccat
     8341 ctctacaaac aataaaaaaa aaaaacagct ggacatggtg gcatgcgcct gtagtggcag
     8401 ataactcagg aggctgaggt ggggggatct gcttgagccc aggaggtgaa ggctgcattg
     8461 agctgtgatt gcaccactgc actccagcct gggtgacaga gcaagaccct gccaaaaaaa
     8521 aaaaaaaaga agaagaaaaa caattttatt ggtagaggcg gggggaataa tagagataca
     8581 gcaccagctc cctgcctcta aaatgtgggc acctgggtaa ctagatagcg caggaagtta
     8641 gaacaaataa aagcagcaaa ctctgtgact gaagtttcag cacatggatg gcaaatattt
     8701 tcaagtttgg tgaaaatagg taagagatca aagcccaaga ataaaccctg gggtcattac
     8761 aggatttcct gtaaaggcat caggaaatgt ttttggaaga atgtgattgt cttcgttgat
     8821 atcaacccaa agatgtggtt attgttcaga atgtcctgat tttctttaaa tgttttgttt
     8881 ctatctgacc tctcattata aatgagaaac ttataaatta taaatataca ctcattataa
     8941 atgaagattg cttggtaatg gactaatgga taattgtttc tgcttcttta ggatgttcta
     9001 aatttttccc aagttttgta cattaagcac aaaattataa caggaaaata aagaaaagaa
     9061 tttaaaataa acatgcaacc aaattttaaa cagataagta tatcatatta gataggaatc
     9121 accatactgg gtcagtcacc cctcctccaa atttttcttt attctttttt ttttattata
     9181 ctttaagttt tagggtacat gtgcacaacg tgcaggtttg ttacatatgt atacatgtgc
     9241 catgttggtg tgctgtaccc attaactcgt catttaccat taggtatatc tcctaatgct
     9301 atccctcccc cctcccccca ccccacaata ggccctggtg tgtgatgttc cccttcctgt
     9361 gtccatgtga tctcattgtt cgattcccac ctatgagtga gaatacgtgg tgtttggttt
     9421 tttgtccttg tattagtttg ctgagaatga tggtttccag cttcatccat gtccctacaa
     9481 aggacacgaa ctcctcattt tttatggctg catagtattc catggtgtat atgtgccaca
     9541 ttttcttaat ccagtctatc gttgttggac atttgggttg gttccaagtc tttgctattg
     9601 tgaatagtgc cacaataaac atatgtgtgc atgtgtcttt atagcagcat gatttctaat
     9661 cctttgggta tatacccagt aatgggatgg ctgggtcaaa tggtatttct agttctagat
     9721 ccctgaggaa tcgccacact gacttccaca atggttgaac tagtttacag tcccaccaac
     9781 agtgtaaaag tgttcctatt tctccacatc ctctccagca cctgttgttt cctgactttt
     9841 taatgatcgc cattctaact ggtgtgagat ggtatctcat tgtggttttg atttgcattt
     9901 ctctgatggc cagtgatgat gagcattata aatgaagatt gcttggtagt ggactaatgg
     9961 ataattgttt ctgcttcttt aggattttct aaatttttcc caagttttgt atattaagca
    10021 caaaattata acaggaaaat aaagaaaaga atttaaaata aa
//
