LOCUS       NM_001225               1296 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens caspase 4 (CASP4), transcript variant alpha, mRNA.
ACCESSION   NM_001225
VERSION     NM_001225.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Cai,R., Gong,X., Li,X., Jiang,Y., Deng,S., Tang,J., Ge,H., Wu,C.,
            Tang,H., Wang,G., Xie,L., Chen,X., Hu,X. and Feng,J.
  TITLE     Dectin-1 aggravates neutrophil inflammation through
            caspase-11/4-mediated macrophage pyroptosis in asthma
  JOURNAL   Respir Res 25 (1), 119 (2024)
   PUBMED   38459541
  REMARK    GeneRIF: Dectin-1 aggravates neutrophil inflammation through
            caspase-11/4-mediated macrophage pyroptosis in asthma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Ulrich,C., Canim,Z., Herberger,E., Girndt,M. and Fiedler,R.
  TITLE     Inflammation in Hypervolemic Hemodialysis Patients: The Roles of
            RelB and Caspase-4
  JOURNAL   Int J Mol Sci 24 (24), 17550 (2023)
   PUBMED   38139378
  REMARK    GeneRIF: Inflammation in Hypervolemic Hemodialysis Patients: The
            Roles of RelB and Caspase-4.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1296)
  AUTHORS   Shi,X., Sun,Q., Hou,Y., Zeng,H., Cao,Y., Dong,M., Ding,J. and
            Shao,F.
  TITLE     Recognition and maturation of IL-18 by caspase-4 noncanonical
            inflammasome
  JOURNAL   Nature 624 (7991), 442-450 (2023)
   PUBMED   37993714
REFERENCE   4  (bases 1 to 1296)
  AUTHORS   Devant,P., Dong,Y., Mintseris,J., Ma,W., Gygi,S.P., Wu,H. and
            Kagan,J.C.
  TITLE     Structural insights into cytokine cleavage by inflammatory
            caspase-4
  JOURNAL   Nature 624 (7991), 451-459 (2023)
   PUBMED   37993712
  REMARK    GeneRIF: Structural insights into cytokine cleavage by inflammatory
            caspase-4.
            Erratum:[Nature. 2024 Jan;625(7995):E17. doi:
            10.1038/s41586-023-06942-4.. PMID: 38172642]
REFERENCE   5  (bases 1 to 1296)
  AUTHORS   Kroken,A.R., Klein,K.A., Mitchell,P.S., Nieto,V., Jedel,E.J.,
            Evans,D.J. and Fleiszig,S.M.J.
  TITLE     Intracellular replication of Pseudomonas aeruginosa in epithelial
            cells requires suppression of the caspase-4 inflammasome
  JOURNAL   mSphere 8 (5), e0035123 (2023)
   PUBMED   37589460
  REMARK    GeneRIF: Intracellular replication of Pseudomonas aeruginosa in
            epithelial cells requires suppression of the caspase-4
            inflammasome.
REFERENCE   6  (bases 1 to 1296)
  AUTHORS   Srinivasula,S.M., Ahmad,M., Fernandes-Alnemri,T., Litwack,G. and
            Alnemri,E.S.
  TITLE     Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1
            protease Mch5 is a CrmA-inhibitable protease that activates
            multiple Ced-3/ICE-like cysteine proteases
  JOURNAL   Proc Natl Acad Sci U S A 93 (25), 14486-14491 (1996)
   PUBMED   8962078
REFERENCE   7  (bases 1 to 1296)
  AUTHORS   Munday,N.A., Vaillancourt,J.P., Ali,A., Casano,F.J., Miller,D.K.,
            Molineaux,S.M., Yamin,T.T., Yu,V.L. and Nicholson,D.W.
  TITLE     Molecular cloning and pro-apoptotic activity of ICErelII and
            ICErelIII, members of the ICE/CED-3 family of cysteine proteases
  JOURNAL   J Biol Chem 270 (26), 15870-15876 (1995)
   PUBMED   7797592
REFERENCE   8  (bases 1 to 1296)
  AUTHORS   Kamens,J., Paskind,M., Hugunin,M., Talanian,R.V., Allen,H.,
            Banach,D., Bump,N., Hackett,M., Johnston,C.G., Li,P. et al.
  TITLE     Identification and characterization of ICH-2, a novel member of the
            interleukin-1 beta-converting enzyme family of cysteine proteases
  JOURNAL   J Biol Chem 270 (25), 15250-15256 (1995)
   PUBMED   7797510
REFERENCE   9  (bases 1 to 1296)
  AUTHORS   Faucheu,C., Diu,A., Chan,A.W., Blanchet,A.M., Miossec,C., Herve,F.,
            Collard-Dutilleul,V., Gu,Y., Aldape,R.A., Lippke,J.A. et al.
  TITLE     A novel human protease similar to the interleukin-1 beta converting
            enzyme induces apoptosis in transfected cells
  JOURNAL   EMBO J 14 (9), 1914-1922 (1995)
   PUBMED   7743998
REFERENCE   10 (bases 1 to 1296)
  AUTHORS   Howard,A.D., Kostura,M.J., Thornberry,N., Ding,G.J., Limjuco,G.,
            Weidner,J., Salley,J.P., Hogquist,K.A., Chaplin,D.D., Mumford,R.A.
            et al.
  TITLE     IL-1-converting enzyme requires aspartic acid residues for
            processing of the IL-1 beta precursor at two distinct sites and
            does not cleave 31-kDa IL-1 alpha
  JOURNAL   J Immunol 147 (9), 2964-2969 (1991)
   PUBMED   1919001
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CN261701.1, Z48810.1,
            AI128358.1 and AP002004.4.
            
            On Nov 22, 2018 this sequence version replaced NM_001225.3.
            
            Summary: This gene encodes a protein that is a member of the
            cysteine-aspartic acid protease (caspase) family. Sequential
            activation of caspases plays a central role in the execution-phase
            of cell apoptosis. Caspases exist as inactive proenzymes composed
            of a prodomain and a large and small protease subunit. Activation
            of caspases requires proteolytic processing at conserved internal
            aspartic residues to generate a heterodimeric enzyme consisting of
            the large and small subunits. This caspase is able to cleave and
            activate its own precursor protein, as well as caspase 1 precursor.
            When overexpressed, this gene induces cell apoptosis. Alternative
            splicing results in transcript variants encoding distinct isoforms.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (alpha) encodes the longest
            isoform (alpha).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008060.2, AK057094.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000444739.7/ ENSP00000388566.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 CN261701.1         54-61
            9-903               Z48810.1           1-895
            904-1295            AI128358.1         1-392               c
            1296-1296           AP002004.4         138687-138687       c
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q22.3"
     gene            1..1296
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /note="caspase 4"
                     /db_xref="GeneID:837"
                     /db_xref="HGNC:HGNC:1505"
                     /db_xref="MIM:602664"
     exon            1..56
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..1183
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /EC_number="3.4.22.57"
                     /note="isoform alpha precursor is encoded by transcript
                     variant alpha; apoptotic cysteine protease Mih1/TX;
                     caspase 4, apoptosis-related cysteine protease; CASP-4;
                     ICE(rel)-II; protease TX; protease ICH-2; caspase 4,
                     apoptosis-related cysteine peptidase; ICE and Ced-3
                     homolog 2"
                     /codon_start=1
                     /product="caspase-4 isoform alpha precursor"
                     /protein_id="NP_001216.1"
                     /db_xref="CCDS:CCDS8327.1"
                     /db_xref="GeneID:837"
                     /db_xref="HGNC:HGNC:1505"
                     /db_xref="MIM:602664"
                     /translation="MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKK
                     YYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGES
                     TDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDIT
                     GMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICG
                     TVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLE
                     VASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYS
                     WCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN"
     exon            57..311
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     exon            312..421
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     exon            422..595
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     exon            596..830
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     exon            831..974
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1084
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1188
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1296
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1275..1280
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /note="hexamer: AATAAA"
     polyA_site      1296
                     /gene="CASP4"
                     /gene_synonym="ICE(rel)II; ICEREL-II; ICH-2; Mih1;
                     Mih1/TX; TX"
                     /note="major polyA site"
ORIGIN      
        1 actctgaggc tctttccaac gctgtaaaaa aggacagagg ctgttcccta tggcagaagg
       61 caaccacaga aaaaagccac ttaaggtgtt ggaatccctg ggcaaagatt tcctcactgg
      121 tgttttggat aacttggtgg aacaaaatgt actgaactgg aaggaagagg aaaaaaagaa
      181 atattacgat gctaaaactg aagacaaagt tcgggtcatg gcagactcta tgcaagagaa
      241 gcaacgtatg gcaggacaaa tgcttcttca aacctttttt aacatagacc aaatatcccc
      301 caataaaaaa gctcatccga atatggaggc tggaccacct gagtcaggag aatctacaga
      361 tgccctcaag ctttgtcctc atgaagaatt cctgagacta tgtaaagaaa gagctgaaga
      421 gatctatcca ataaaggaga gaaacaaccg cacacgcctg gctctcatca tatgcaatac
      481 agagtttgac catctgcctc cgaggaatgg agctgacttt gacatcacag ggatgaagga
      541 gctacttgag ggtctggact atagtgtaga tgtagaagag aatctgacag ccagggatat
      601 ggagtcagcg ctgagggcat ttgctaccag accagagcac aagtcctctg acagcacatt
      661 cttggtactc atgtctcatg gcatcctgga gggaatctgc ggaactgtgc atgatgagaa
      721 aaaaccagat gtgctgcttt atgacaccat cttccagata ttcaacaacc gcaactgcct
      781 cagtctgaag gacaaaccca aggtcatcat tgtccaggcc tgcagaggtg caaaccgtgg
      841 ggaactgtgg gtcagagact ctccagcatc cttggaagtg gcctcttcac agtcatctga
      901 gaacctagag gaagatgctg tttacaagac ccacgtggag aaggacttca ttgctttctg
      961 ctcttcaacg ccacacaacg tgtcctggag agacagcaca atgggctcta tcttcatcac
     1021 acaactcatc acatgcttcc agaaatattc ttggtgctgc cacctagagg aagtatttcg
     1081 gaaggtacag caatcatttg aaactccaag ggccaaagct caaatgccca ccatagaacg
     1141 actgtccatg acaagatatt tctacctctt tcctggcaat tgaaaatgga agccacaagc
     1201 agcccagccc tccttaatca acttcaagga gcaccttcat tagtacagct tgcatattta
     1261 acattttgta tttcaataaa agtgaagaca aacgaa
//
