LOCUS       NM_004346               2645 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens caspase 3 (CASP3), transcript variant 1, mRNA.
ACCESSION   NM_004346 NM_001354778
VERSION     NM_004346.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Yang,J., Li,Y., Li,H., Zhang,H., Guo,H., Zheng,X., Yu,X.F. and
            Wei,W.
  TITLE     HIV-1 Vpu induces neurotoxicity by promoting Caspase 3-dependent
            cleavage of TDP-43
  JOURNAL   EMBO Rep 25 (10), 4337-4357 (2024)
   PUBMED   39242776
  REMARK    GeneRIF: HIV-1 Vpu induces neurotoxicity by promoting Caspase
            3-dependent cleavage of TDP-43.
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Yu,G., Chen,X., Lu,W., Li,Y., Chen,Y., Yin,C., Zheng,Z., Huang,X.
            and Xu,D.
  TITLE     Decreasing circ_0014614 promotes the differentiation of bone marrow
            flineage cells into megakaryocytes in essential thrombocythemia via
            activiation of miR-138-5p/caspase3 axis
  JOURNAL   Blood Cells Mol Dis 107, 102855 (2024)
   PUBMED   38703475
  REMARK    GeneRIF: Decreasing circ_0014614 promotes the differentiation of
            bone marrow flineage cells into megakaryocytes in essential
            thrombocythemia via activiation of miR-138-5p/caspase3 axis.
REFERENCE   3  (bases 1 to 2645)
  AUTHORS   Ghorbani,N., Shiri,M., Alian,M., Yaghubi,R., Shafaghi,M.,
            Hojjat,H., Pahlavan,S. and Davoodi,J.
  TITLE     A Non-Apoptotic Pattern of Caspase-9/Caspase-3 Activation During
            Differentiation of Human Embryonic Stem Cells into Cardiomyocytes
  JOURNAL   Adv Biol (Weinh) 8 (6), e2400026 (2024)
   PUBMED   38640919
  REMARK    GeneRIF: A Non-Apoptotic Pattern of Caspase-9/Caspase-3 Activation
            During Differentiation of Human Embryonic Stem Cells into
            Cardiomyocytes.
REFERENCE   4  (bases 1 to 2645)
  AUTHORS   Yuan,H., Yi,N., Li,D., Xu,C., Yin,G.R., Zhuang,C., Wang,Y.J. and
            Ni,S.
  TITLE     PPARgamma regulates osteoarthritis chondrocytes apoptosis through
            caspase-3 dependent mitochondrial pathway
  JOURNAL   Sci Rep 14 (1), 11237 (2024)
   PUBMED   38755283
  REMARK    GeneRIF: PPARgamma regulates osteoarthritis chondrocytes apoptosis
            through caspase-3 dependent mitochondrial pathway.
            Erratum:[Sci Rep. 2024 Jun 10;14(1):13263. doi:
            10.1038/s41598-024-64106-4.. PMID: 38858431]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2645)
  AUTHORS   Lv,H., Liu,P., Hu,H., Li,X. and Li,P.
  TITLE     MiR-98-5p plays suppressive effects on IL-1beta-induced chondrocyte
            injury associated with osteoarthritis by targeting CASP3
  JOURNAL   J Orthop Surg Res 19 (1), 239 (2024)
   PUBMED   38615043
  REMARK    GeneRIF: MiR-98-5p plays suppressive effects on IL-1beta-induced
            chondrocyte injury associated with osteoarthritis by targeting
            CASP3.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2645)
  AUTHORS   Nicholson,D.W., Ali,A., Thornberry,N.A., Vaillancourt,J.P.,
            Ding,C.K., Gallant,M., Gareau,Y., Griffin,P.R., Labelle,M.,
            Lazebnik,Y.A. et al.
  TITLE     Identification and inhibition of the ICE/CED-3 protease necessary
            for mammalian apoptosis
  JOURNAL   Nature 376 (6535), 37-43 (1995)
   PUBMED   7596430
REFERENCE   7  (bases 1 to 2645)
  AUTHORS   Tewari,M., Quan,L.T., O'Rourke,K., Desnoyers,S., Zeng,Z.,
            Beidler,D.R., Poirier,G.G., Salvesen,G.S. and Dixit,V.M.
  TITLE     Yama/CPP32 beta, a mammalian homolog of CED-3, is a
            CrmA-inhibitable protease that cleaves the death substrate
            poly(ADP-ribose) polymerase
  JOURNAL   Cell 81 (5), 801-809 (1995)
   PUBMED   7774019
REFERENCE   8  (bases 1 to 2645)
  AUTHORS   Fernandes-Alnemri,T., Litwack,G. and Alnemri,E.S.
  TITLE     CPP32, a novel human apoptotic protein with homology to
            Caenorhabditis elegans cell death protein Ced-3 and mammalian
            interleukin-1 beta-converting enzyme
  JOURNAL   J Biol Chem 269 (49), 30761-30764 (1994)
   PUBMED   7983002
REFERENCE   9  (bases 1 to 2645)
  AUTHORS   Walker,N.P., Talanian,R.V., Brady,K.D., Dang,L.C., Bump,N.J.,
            Ferenz,C.R., Franklin,S., Ghayur,T., Hackett,M.C., Hammill,L.D. et
            al.
  TITLE     Crystal structure of the cysteine protease interleukin-1
            beta-converting enzyme: a (p20/p10)2 homodimer
  JOURNAL   Cell 78 (2), 343-352 (1994)
   PUBMED   8044845
REFERENCE   10 (bases 1 to 2645)
  AUTHORS   Kopp,S.
  TITLE     Reproducibility of response to a questionnaire on symptoms of
            masticatory dysfunction
  JOURNAL   Community Dent Oral Epidemiol 4 (5), 205-209 (1976)
   PUBMED   1067155
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU127028.1, BC016926.2 and
            BU753483.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004346.3.
            
            Summary: The protein encoded by this gene is a cysteine-aspartic
            acid protease that plays a central role in the execution-phase of
            cell apoptosis. The encoded protein cleaves and inactivates
            poly(ADP-ribose) polymerase while it cleaves and activates sterol
            regulatory element binding proteins as well as caspases 6, 7, and
            9. This protein itself is processed by caspases 8, 9, and 10. It is
            the predominant caspase involved in the cleavage of amyloid-beta 4A
            precursor protein, which is associated with neuronal death in
            Alzheimer's disease. [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (1, also known as alpha) encodes
            the longest isoform (a). Variants 1, 2, and 3 all encode the same
            isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.181796.1,
                                           SRR1803617.172427.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308394.9/ ENSP00000311032.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AU127028.1         29-78
            51-2485             BC016926.2         31-2465
            2486-2645           BU753483.1         17-176              c
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q35.1"
     gene            1..2645
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /note="caspase 3"
                     /db_xref="GeneID:836"
                     /db_xref="HGNC:HGNC:1504"
                     /db_xref="MIM:600636"
     exon            1..53
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /inference="alignment:Splign:2.1.0"
     exon            54..220
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /inference="alignment:Splign:2.1.0"
     exon            221..288
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             236..1069
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /EC_number="3.4.22.56"
                     /note="isoform a preproprotein is encoded by transcript
                     variant 1; caspase 3, apoptosis-related cysteine protease;
                     PARP cleavage protease; cysteine protease CPP32; SREBP
                     cleavage activity 1; procaspase3; apopain; CASP-3; CPP-32;
                     protein Yama; caspase 3, apoptosis-related cysteine
                     peptidase"
                     /codon_start=1
                     /product="caspase-3 isoform a preproprotein"
                     /protein_id="NP_004337.2"
                     /db_xref="CCDS:CCDS3836.1"
                     /db_xref="GeneID:836"
                     /db_xref="HGNC:HGNC:1504"
                     /db_xref="MIM:600636"
                     /translation="MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEM
                     GLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRD
                     VSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
                     QACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQS
                     LCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFY
                     H"
     exon            289..413
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /inference="alignment:Splign:2.1.0"
     exon            414..542
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..718
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /inference="alignment:Splign:2.1.0"
     exon            719..839
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /inference="alignment:Splign:2.1.0"
     exon            840..2645
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2622..2627
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /note="hexamer: AATATA"
     polyA_site      2645
                     /gene="CASP3"
                     /gene_synonym="CPP32; CPP32B; SCA-1"
                     /note="major polyA site"
ORIGIN      
        1 agtgcagacg cggctcctag cggatgggtg ctattgtgag gcggttgtag aagagtttcg
       61 tgagtgctcg cagctcatac ctgtggctgt gtatccgtgg ccacagctgg ttggcgtcgc
      121 cttgaaatcc caggccgtga ggagttagcg agccctgctc acactcggcg ctctggtttt
      181 cggtgggtgt gccctgcacc tgcctcttcc cccattctca ttaataaagg tatccatgga
      241 gaacactgaa aactcagtgg attcaaaatc cattaaaaat ttggaaccaa agatcataca
      301 tggaagcgaa tcaatggact ctggaatatc cctggacaac agttataaaa tggattatcc
      361 tgagatgggt ttatgtataa taattaataa taagaatttt cataaaagca ctggaatgac
      421 atctcggtct ggtacagatg tcgatgcagc aaacctcagg gaaacattca gaaacttgaa
      481 atatgaagtc aggaataaaa atgatcttac acgtgaagaa attgtggaat tgatgcgtga
      541 tgtttctaaa gaagatcaca gcaaaaggag cagttttgtt tgtgtgcttc tgagccatgg
      601 tgaagaagga ataatttttg gaacaaatgg acctgttgac ctgaaaaaaa taacaaactt
      661 tttcagaggg gatcgttgta gaagtctaac tggaaaaccc aaacttttca ttattcaggc
      721 ctgccgtggt acagaactgg actgtggcat tgagacagac agtggtgttg atgatgacat
      781 ggcgtgtcat aaaataccag tggaggccga cttcttgtat gcatactcca cagcacctgg
      841 ttattattct tggcgaaatt caaaggatgg ctcctggttc atccagtcgc tttgtgccat
      901 gctgaaacag tatgccgaca agcttgaatt tatgcacatt cttacccggg ttaaccgaaa
      961 ggtggcaaca gaatttgagt ccttttcctt tgacgctact tttcatgcaa agaaacagat
     1021 tccatgtatt gtttccatgc tcacaaaaga actctatttt tatcactaaa gaaatggttg
     1081 gttggtggtt ttttttagtt tgtatgccaa gtgagaagat ggtatatttg gtactgtatt
     1141 tccctctcat tttgacctac tctcatgctg cagagggtac tttaagacat actccttcca
     1201 tcaaatagaa ccactatgaa gctacctcaa acttccagtc aggtagttgc aattgaatta
     1261 aattaggaat aaataaaaat ggatactggt gcagtcatta tgagaggcaa tgattgttaa
     1321 tttacagctt tcatgattag caagttacag tgatgctgtg ctatgaattt tcaagtaatt
     1381 gtgaaaaagt taaacattga agtaatgaat ttttatgata ttccccccac ttaagactgt
     1441 gtattctagt tttgtcaaac tgtagaaatg atgatgtgga agaacttagg catctgtggg
     1501 catggtcaaa ggctcaaacc tttattttag aattgatata cacggatgac ttaactgcat
     1561 ttttagacca tttatctggg attatggttt tgtgatgttt gtcctgaaca cttttgttgt
     1621 aaaaaaataa taataatgtt taatattgag aaagaaacta atattttatg tgagagaaag
     1681 tgtgagcaaa ctaacttgac ttttaaggct aaaacttaac attcatagag gggtggagtt
     1741 ttaactgtaa ggtgctacaa tgcccctgga tctaccagca taaatatctt ctgatttgtc
     1801 cctatgcata tcagttgagc ttcatatacc agcaatatat ctgaagagct attatataaa
     1861 aaccccaaac tgttgattat tagccaggta atgtgaataa attctatagg aacatatgaa
     1921 aatacaactt aaataataaa cagtggaata taaggaaagc aataaatgaa tgggctgagc
     1981 tgcctgtaac ttgagagtag atggtttgag cctgagcaga gacatgactc agcctgttcc
     2041 atgaaggcag agccatggac cacgcaggaa gggcctacag cccatttctc catacgcact
     2101 ggtatgtgtg gatgatgctg ccagggcgcc atcgccaagt aagaaagtga agcaaatcag
     2161 aaacttgtga agtggaaatg ttctaaaggt ggtgaggcaa taaaaatcat agtactcttt
     2221 gtagcaaaat tcttaagtat gttattttct gttgaagttt acaatcaaag gaaaatagta
     2281 atgttttata ctgtttactg aaagaaaaag acctatgagc acataggact ctagacggca
     2341 tccagccgga ggccagagct gagccctcag cccgggaggc aggctccagg cctcagcagg
     2401 tgcggagccg tcactgcacc aagtctcact ggctgtcagt atgacatttc acgggagatt
     2461 tcttgttgct caaaaaatga gctcgcattt gtcaatgaca gtttcttttt tcttactaga
     2521 cctgtaactt ttgtaaatac acatagcatg taatggtatc ttaaagtgtg tttctatgtg
     2581 acaattttgt acaaatttgt tattttccat ttttatttca aaatatacat tcaaacttaa
     2641 aatta
//
