LOCUS       NM_020753               4969 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens CASK interacting protein 2 (CASKIN2), transcript
            variant 1, mRNA.
ACCESSION   NM_020753
VERSION     NM_020753.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Wang,W., Atherton,P., Kreft,M., Te Molder,L., van der Poel,S.,
            Hoekman,L., Celie,P., Joosten,R.P., Fassler,R., Perrakis,A. and
            Sonnenberg,A.
  TITLE     Caskin2 is a novel talin- and Abi1-binding protein that promotes
            cell motility
  JOURNAL   J Cell Sci 137 (9) (2024)
   PUBMED   38587458
  REMARK    GeneRIF: Caskin2 is a novel talin- and Abi1-binding protein that
            promotes cell motility.
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Kwan,J.J. and Donaldson,L.W.
  TITLE     A lack of peptide binding and decreased thermostability suggests
            that the CASKIN2 scaffolding protein SH3 domain may be vestigial
  JOURNAL   BMC Struct Biol 16 (1), 14 (2016)
   PUBMED   27619958
  REMARK    GeneRIF: While the NMR structure demonstrates that the CASKIN2 SH3
            domain is folded, its cleft has suffered two substitutions that
            prevent it from binding typical polyproline ligands.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4969)
  AUTHORS   Knight,M.J., Leettola,C., Gingery,M., Li,H. and Bowie,J.U.
  TITLE     A human sterile alpha motif domain polymerizome
  JOURNAL   Protein Sci 20 (10), 1697-1706 (2011)
   PUBMED   21805519
REFERENCE   4  (bases 1 to 4969)
  AUTHORS   Tabuchi,K., Biederer,T., Butz,S. and Sudhof,T.C.
  TITLE     CASK participates in alternative tripartite complexes in which Mint
            1 competes for binding with caskin 1, a novel CASK-binding protein
  JOURNAL   J Neurosci 22 (11), 4264-4273 (2002)
   PUBMED   12040031
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC066643.1, DA336467.1 and
            DA538297.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_020753.4.
            
            Summary: This gene encodes a large protein that contains six
            ankyrin repeats, as well as a Src homology 3 (SH3) domain and two
            sterile alpha motif (SAM) domains, which may be involved in
            protein-protein interactions. The C-terminal portion of this
            protein is proline-rich and contains a conserved region. A related
            protein interacts with calcium/calmodulin-dependent serine protein
            kinase (CASK). Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Mar 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066643.1, AF451976.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000321617.8/ ENSP00000325355.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-414               BC066643.1         10-423
            415-647             DA336467.1         352-584
            648-3072            BC066643.1         657-3081
            3073-3577           DA538297.1         65-569
            3578-4969           BC066643.1         3587-4978
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..4969
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /note="CASK interacting protein 2"
                     /db_xref="GeneID:57513"
                     /db_xref="HGNC:HGNC:18200"
                     /db_xref="MIM:612185"
     exon            1..137
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            138..635
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     CDS             542..4150
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /note="isoform a is encoded by transcript variant 1;
                     caskin-2"
                     /codon_start=1
                     /product="caskin-2 isoform a"
                     /protein_id="NP_065804.2"
                     /db_xref="CCDS:CCDS11723.1"
                     /db_xref="GeneID:57513"
                     /db_xref="HGNC:HGNC:18200"
                     /db_xref="MIM:612185"
                     /translation="MGREQDLILAVKNGDVTGVQKLVAKVKATKTKLLGSTKRLNVNY
                     QDADGFSALHHAALGGSLELIALLLEAQATVDIKDSNGMRPLHYAAWQGRLEPVRLLL
                     RASAAVNAASLDGQIPLHLAAQYGHYEVSEMLLQHQSNPCLVNKAKKTPLDLACEFGR
                     LKVAQLLLNSHLCVALLEGEAKDPCDPNYTTPLHLAAKNGHREVIRQLLRAGIEINRQ
                     TKTGTALHEAALYGKTEVVRLLLEGGVDVNIRNTYNQTALDIVNQFTTSQASREIKQL
                     LREASGILKVRALKDFWNLHDPTALNVRAGDVITVLEQHPDGRWKGHIHESQRGTDRI
                     GYFPPGIVEVVSKRVGIPAARLPSAPTPLRPGFSRTPQPPAEEPPHPLTYSQLPRVGL
                     SPDSPAGDRNSVGSEGSVGSIRSAGSGQSSEGTNGHGPGLLIENAQPLPSAGEDQVLP
                     GLHPPSLADNLSHRPLANCRSGEQIFTQDVRPEQLLEGKDAQAIHNWLSEFQLEGYTA
                     HFLQAGYDVPTISRMTPEDLTAIGVTKPGHRKKIASEIAQLSIAEWLPSYIPTDLLEW
                     LCALGLPQYHKQLVSSGYDSMGLVADLTWEELQEIGVNKLGHQKKLMLGVKRLAELRR
                     GLLQGEALSEGGRRLAKGPELMAIEGLENGEGPATAGPRLLTFQGSELSPELQAAMAG
                     GGPEPLPLPPARSPSQESIGARSRGSGHSQEQPAPQPSGGDPSPPQERNLPEGTERPP
                     KLCSSLPGQGPPPYVFMYPQGSPSSPAPGPPPGAPWAFSYLAGPPATPPDPPRPKRRS
                     HSLSRPGPTEGDAEGEAEGPVGSTLGSYATLTRRPGRSALVRTSPSVTPTPARGTPRS
                     QSFALRARRKGPPPPPPKRLSSVSGPSPEPPPLDESPGPKEGATGPRRRTLSEPAGPS
                     EPPGPPAPAGPASDTEEEEPGPEGTPPSRGSSGEGLPFAEEGNLTIKQRPKPAGPPPR
                     ETPVPPGLDFNLTESDTVKRRPKCREREPLQTALLAFGVASATPGPAAPLPSPTPGES
                     PPASSLPQPEPSSLPAQGVPTPLAPSPAMQPPVPPCPGPGLESSAASRWNGETEPPAA
                     PAALLKVPGAGTAPKPVSVACTQLAFSGPKLAPRLGPRPVPPPRPESTGTVGPGQAQQ
                     RLEQTSSSLAAALRAAEKSIGTKEQEGTPSASTKHILDDISTMFDALADQLDAMLD"
     exon            636..687
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            688..785
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            786..931
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1027
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1158
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1267
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1376
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1471
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1733
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1855
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..1916
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2008
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2119
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2221
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            2222..2360
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..3836
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            3837..4059
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     exon            4060..4969
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4947..4952
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /note="hexamer: AATGAA"
     polyA_site      4969
                     /gene="CASKIN2"
                     /gene_synonym="ANKS5B"
                     /note="major polyA site"
ORIGIN      
        1 gggatttcct gcgggaggcg ccgagccggc cttggaaaag gagaaggaga aaggggggca
       61 ggggggagtg ggagccaccg tcctgagtgc cagggaccga gcagggccag ccggaccccg
      121 gcgggcggcc gggagagagc cctgagacca gtgcaccgct gctgcctgag gcccaaggga
      181 atgctggctg ctctctgggt gggcactggt gttccacagc caagccggga agattggtgt
      241 gcccagtccc agcccatcct ggagctgtcg gaagccacaa gctgatatcc atccagattt
      301 ccatgtagcc ttcaccccct caaagattag ctttccttcg tgtggtggca gttcacggag
      361 cacctttggg agtatccctc agtacctgac gaggagccca ctgcctggga cacctgactt
      421 gctcccccgg aaccgggagc cctccaaggg gctgtggcat ggcgccctga cgccccagct
      481 tggacccgtt gcctgccttt gccctggact cctgagtgta gagctcagcc caggagccag
      541 tatgggtcgt gaacaggacc tgatcctcgc cgtcaagaat ggagatgtga ccggtgtgca
      601 gaaactggtg gcgaaggtca aggccacaaa gacaaagctc ctgggctcca caaagaggct
      661 caacgtgaac taccaggatg ctgatggatt ctctgccctc caccacgctg ctttgggggg
      721 cagcctggag ctcatagcct tgctgctaga ggctcaggcc actgttgaca tcaaggacag
      781 caatggcatg cgcccgctgc actacgcagc ctggcagggc cggctggagc ctgtgaggct
      841 gctgctgcgc gcctctgcgg ctgtcaatgc cgcctcgctg gacggacaga tccccctgca
      901 cctggctgca cagtatggac attatgaggt gtcagaaatg ctcctccagc atcagtccaa
      961 cccatgcctg gtcaacaagg ccaagaagac gcccctggac ctggcctgtg aatttggccg
     1021 actcaaggtg gcccagctgc ttctgaacag ccacttatgt gtggcactgc tggagggtga
     1081 ggccaaagac ccgtgtgacc ccaactacac cacgcccctg cacttggctg ccaagaatgg
     1141 ccacagagaa gtcatcaggc agctcctgag agctgggatc gagatcaacc gccagaccaa
     1201 gacgggtacg gcgctccacg aggccgcact gtatggcaag accgaggtgg tgcggctgct
     1261 tctggaggga ggtgtggacg tgaacatccg gaatacgtat aaccagacgg cgctggacat
     1321 agtgaatcag ttcaccacct cccaggccag ccgggaaatc aagcagctac tgcgggaggc
     1381 ctcagggatc ctgaaggtcc gagcgctcaa ggatttctgg aacctccacg atcccactgc
     1441 tctcaatgtc cgggcagggg atgtcatcac ggtgctagaa cagcatcccg acggccgctg
     1501 gaagggccac atccacgaga gccagagggg cacagaccgc ataggctact tccccccggg
     1561 cattgtcgag gtggtcagca agcgggtggg catccctgca gcccgcctcc cctccgcacc
     1621 cacccccctg cgcccaggct tctcccggac accgcagcct cctgccgaag aacccccgca
     1681 ccctcttacc tacagccagc ttcctcgggt gggcctcagc ccagacagcc cagcaggtga
     1741 caggaatagt gtgggcagtg agggcagcgt gggcagcatc cgcagtgccg gcagcgggca
     1801 gagctctgag ggcactaatg gccatggccc tggcctcctg attgagaacg cccagccact
     1861 gccctctgct ggagaggacc aggtgctgcc aggactccac ccgccgtccc tggcagacaa
     1921 cctgagccac cgccctctgg ccaactgccg ctctggggag cagatcttca cccaggacgt
     1981 gcggccagaa cagctgctgg aggggaagga cgcgcaggcc attcataact ggctaagcga
     2041 gttccagctg gagggctaca ctgcccactt tctgcaggcc ggctatgatg tgcctaccat
     2101 cagccgcatg acacctgagg acctgacggc catcggggtg accaagcctg ggcacaggaa
     2161 gaagatcgcc tcagagatcg ctcagctcag catcgccgag tggctgccca gctacatccc
     2221 aacggacctg ctggagtggc tgtgtgcact ggggctgcca cagtaccaca agcagctggt
     2281 gagcagcggc tacgactcca tggggctggt ggccgacctc acctgggagg agctgcagga
     2341 gattggggtc aacaagctcg ggcatcagaa gaagctcatg ctgggggtga agcggctggc
     2401 ggagcttcgg cggggcctgc tgcaggggga ggccctcagc gaaggcgggc gccggctggc
     2461 caagggtccg gagctgatgg ccatcgaggg actggagaac ggagaaggcc cagctacagc
     2521 tggcccacgg ctcctcacct tccagggcag cgaactaagc ccagagctac aggcggccat
     2581 ggcagggggt ggccctgaac cactccccct cccacctgcc cgctctccca gccaggagag
     2641 catcggggca cgctcacggg ggtctggcca ctcacaggaa cagcctgccc cacagcccag
     2701 cggtggagat cccagccccc cccaggagag gaacctccca gagggcacag agcggccccc
     2761 taagctttgt tcttcacttc ctggccaagg acccccaccc tatgttttta tgtaccccca
     2821 gggctcaccc tctagcccgg ccccagggcc acctcctggc gcaccctggg ccttctccta
     2881 cttggccggg ccccctgcca ctcccccaga cccgcctcga cctaagcgcc ggtcccacag
     2941 cctaagccgc cctggcccca cagaggggga tgctgagggg gaggccgaag ggccagtggg
     3001 cagcacccta ggcagttatg ctacccttac ccggcggcca ggacgcagtg cccttgtccg
     3061 gaccagtcct agtgtgaccc caaccccagc tcgggggact cctcgcagcc agtcctttgc
     3121 cctgcgggcc cggcgcaaag gccccccgcc cccgcccccc aagcgcctca gctccgtctc
     3181 tggccccagc ccggagccac ctccactaga tgagagccca gggcccaagg aaggggccac
     3241 agggccccga aggcgaacac tgagtgaacc tgctggcccc tcagagcccc ctggcccacc
     3301 tgccccggct gggcccgcgt cagacacgga ggaggaggag ccaggccctg aggggacgcc
     3361 cccatctcgg ggcagctctg gggaagggct gccgtttgca gaggaaggga acctgaccat
     3421 caaacagcgc ccgaagcccg ctggcccccc gccccgagag acacccgtgc cccccggcct
     3481 cgatttcaac ctcacggaat cagacactgt taagcggagg cccaagtgcc gggagagaga
     3541 gccactgcag accgcactgc tggccttcgg agtggccagt gccacgcctg gccccgctgc
     3601 cccactgcct tccccaactc ctggcgagtc tcctccagct tctagccttc cccagcccga
     3661 gcccagcagc cttccagccc aaggagttcc aacccccctt gctcccagcc ccgccatgca
     3721 gcctccagtg ccgccctgcc cagggccagg tctggaaagc tcagcagcta gtcggtggaa
     3781 tggggagaca gaacccccgg ccgcccctgc tgccctcctc aaggtgcccg gagcaggaac
     3841 agcccccaag cctgtgtcgg tggcctgcac ccagctggca ttttctggcc ctaagctagc
     3901 gccccggctc ggcccccgcc cagtgcctcc tccacggcct gagagcactg ggactgtggg
     3961 cccaggccag gcccagcaga gactggagca gaccagctcg tccctggcag ctgcactgag
     4021 agccgcagag aagagcattg gcaccaagga gcaagagggc acccccagcg cctccaccaa
     4081 gcacattctg gatgacatca gcaccatgtt cgacgccctg gctgaccagc tggacgccat
     4141 gctggactga gccctccagc agtgcccact gtgacctgcc gaagtccact gcctttgccc
     4201 cagcacagaa gaggcccctg ccaccctagg gacgggccaa gggctggtca ggctgaagtg
     4261 cccctcctag cagggcccct tcccactcag cccgcggctg tgggcaccac agctcttgtg
     4321 gggcagccca ccttagaacc tgactagcga gggacctccg ctgcatctca gcaaagcccc
     4381 tcccagggtt tgatcgattg agcaggacag ccctgctcct ggacagggac cctggtaaga
     4441 gctctctcct cagggaggaa gtaggggtgg ggctttgggg gtgctttctc tgtacccccc
     4501 agcccatgtc ccaagttgtg ccaagggaat gcctcttgcc acccaccatc ctgcccaggc
     4561 cctgagaggc tggaggaggc gtgtgtctgg ccgggccccc tctccgtagg tgtacagagg
     4621 acatgtaaat acacagcggt ggtgcccagg cagctcctgc ccccgccttc cctcactgag
     4681 gcagggctag caggggtggg aggcagtggg gttaacagat cccacaagtc ctaacttttc
     4741 aattgtaaat gttattttct aagcagagag aaatgcatat attttaaatg gaatttattc
     4801 tatcaactgc ctgagaggac acaatggggg aggggcttcg gaccacagca ggagccccga
     4861 ctgcccacct gagggcaggg agagcctgac cccattggcc caggccctgg ctctgtaacc
     4921 attaacctct tcccccaact aacaccaatg aaaacaccat tccacgtga
//
