LOCUS       NM_001013838            4462 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens capping protein regulator and myosin 1 linker 2
            (CARMIL2), transcript variant 1, mRNA.
ACCESSION   NM_001013838 XM_085383
VERSION     NM_001013838.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Levy,R., Gothe,F., Momenilandi,M., Magg,T., Materna,M., Peters,P.,
            Raedler,J., Philippot,Q., Rack-Hoch,A.L., Langlais,D., Bourgey,M.,
            Lanz,A.L., Ogishi,M., Rosain,J., Martin,E., Latour,S.,
            Vladikine,N., Distefano,M., Khan,T., Rapaport,F., Schulz,M.S.,
            Holzer,U., Fasth,A., Sogkas,G., Speckmann,C., Troilo,A., Bigley,V.,
            Roppelt,A., Dinur-Schejter,Y., Toker,O., Bronken Martinsen,K.H.,
            Sherkat,R., Somekh,I., Somech,R., Shouval,D.S., Kuhl,J.S., Ip,W.,
            McDermott,E.M., Cliffe,L., Ozen,A., Baris,S., Rangarajan,H.G.,
            Jouanguy,E., Puel,A., Bustamante,J., Alyanakian,M.A., Fusaro,M.,
            Wang,Y., Kong,X.F., Cobat,A., Boutboul,D., Castelle,M., Aguilar,C.,
            Hermine,O., Cheminant,M., Suarez,F., Yildiran,A., Bousfiha,A.,
            Al-Mousa,H., Alsohime,F., Cagdas,D., Abraham,R.S., Knutsen,A.P.,
            Fevang,B., Bhattad,S., Kiykim,A., Erman,B., Arikoglu,T., Unal,E.,
            Kumar,A., Geier,C.B., Baumann,U., Neven,B., Rohlfs,M., Walz,C.,
            Abel,L., Malissen,B., Marr,N., Klein,C., Casanova,J.L., Hauck,F.
            and Beziat,V.
  CONSRTM   CARMIL2 Consortium
  TITLE     Human CARMIL2 deficiency underlies a broader immunological and
            clinical phenotype than CD28 deficiency
  JOURNAL   J Exp Med 220 (2) (2023)
   PUBMED   36515678
  REMARK    GeneRIF: Human CARMIL2 deficiency underlies a broader immunological
            and clinical phenotype than CD28 deficiency.
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Bosa,L., Batura,V., Colavito,D., Fiedler,K., Gaio,P., Guo,C.,
            Li,Q., Marzollo,A., Mescoli,C., Nambu,R., Pan,J., Perilongo,G.,
            Warner,N., Zhang,S., Kotlarz,D., Klein,C., Snapper,S.B.,
            Walters,T.D., Leon,A., Griffiths,A.M., Cananzi,M. and Muise,A.M.
  TITLE     Novel CARMIL2 loss-of-function variants are associated with
            pediatric inflammatory bowel disease
  JOURNAL   Sci Rep 11 (1), 5945 (2021)
   PUBMED   33723309
  REMARK    GeneRIF: Novel CARMIL2 loss-of-function variants are associated
            with pediatric inflammatory bowel disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4462)
  AUTHORS   Uchida,Y., Yoshimitsu,M., Hachiman,M., Kusano,S., Arima,N.,
            Shima,K., Hayashida,M., Kamada,Y., Nakamura,D., Arai,A., Tanaka,Y.,
            Hara,H. and Ishitsuka,K.
  TITLE     RLTPR Q575E: A novel recurrent gain-of-function mutation in
            patients with adult T-cell leukemia/lymphoma
  JOURNAL   Eur J Haematol 106 (2), 221-229 (2021)
   PUBMED   33098696
  REMARK    GeneRIF: RLTPR Q575E: A novel recurrent gain-of-function mutation
            in patients with adult T-cell leukemia/lymphoma.
REFERENCE   4  (bases 1 to 4462)
  AUTHORS   Yonkof,J.R., Gupta,A., Rueda,C.M., Mangray,S., Prince,B.T.,
            Rangarajan,H.G., Alshahrani,M., Varga,E., Cripe,T.P. and
            Abraham,R.S.
  TITLE     A Novel Pathogenic Variant in CARMIL2 (RLTPR) Causing CARMIL2
            Deficiency and EBV-Associated Smooth Muscle Tumors
  JOURNAL   Front Immunol 11, 884 (2020)
   PUBMED   32625199
  REMARK    GeneRIF: A Novel Pathogenic Variant in CARMIL2 (RLTPR) Causing
            CARMIL2 Deficiency and EBV-Associated Smooth Muscle Tumors.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4462)
  AUTHORS   Shamriz,O., Simon,A.J., Lev,A., Megged,O., Ledder,O., Picard,E.,
            Joseph,L., Molho-Pessach,V., Tal,Y., Millman,P., Slae,M.,
            Somech,R., Toker,O. and Berger,M.
  TITLE     Exogenous interleukin-2 can rescue in-vitro T cell activation and
            proliferation in patients with a novel capping protein regulator
            and myosin 1 linker 2 mutation
  JOURNAL   Clin Exp Immunol 200 (3), 215-227 (2020)
   PUBMED   32201938
  REMARK    GeneRIF: Exogenous interleukin-2 can rescue in-vitro T cell
            activation and proliferation in patients with a novel capping
            protein regulator and myosin 1 linker 2 mutation.
REFERENCE   6  (bases 1 to 4462)
  AUTHORS   Lanier,M.H., McConnell,P. and Cooper,J.A.
  TITLE     Cell Migration and Invadopodia Formation Require a Membrane-binding
            Domain of CARMIL2
  JOURNAL   J Biol Chem 291 (3), 1076-1091 (2016)
   PUBMED   26578515
  REMARK    GeneRIF: Data suggest that CARMIL2 displays two localization
            patterns in cells; one coincides with vimentin intermediate
            filaments, and the other coincides with cell membrane at leading
            edge of migrating cells.
REFERENCE   7  (bases 1 to 4462)
  AUTHORS   Lanier,M.H., Kim,T. and Cooper,J.A.
  TITLE     CARMIL2 is a novel molecular connection between vimentin and actin
            essential for cell migration and invadopodia formation
  JOURNAL   Mol Biol Cell 26 (25), 4577-4588 (2015)
   PUBMED   26466680
  REMARK    GeneRIF: On the basis of these results, we propose a model in which
            dynamic vimentin filaments target CARMIL2 to critical
            membrane-associated locations, where CARMIL2 regulates CP, and thus
            actin assembly, to create cell protrusions
REFERENCE   8  (bases 1 to 4462)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   9  (bases 1 to 4462)
  AUTHORS   Liang,Y., Niederstrasser,H., Edwards,M., Jackson,C.E. and
            Cooper,J.A.
  TITLE     Distinct roles for CARMIL isoforms in cell migration
  JOURNAL   Mol Biol Cell 20 (24), 5290-5305 (2009)
   PUBMED   19846667
REFERENCE   10 (bases 1 to 4462)
  AUTHORS   Matsuzaka,Y., Okamoto,K., Mabuchi,T., Iizuka,M., Ozawa,A., Oka,A.,
            Tamiya,G., Kulski,J.K. and Inoko,H.
  TITLE     Identification, expression analysis and polymorphism of a novel
            RLTPR gene encoding a RGD motif, tropomodulin domain and
            proline/leucine-rich regions
  JOURNAL   Gene 343 (2), 291-304 (2004)
   PUBMED   15588584
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB113647.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001013838.2.
            
            Summary: This gene encodes a member of the CARMIL (capping protein,
            Arp2/3, myosin-I linker) family of proteins. The encoded protein
            interacts with and negatively regulates the heterodimeric capping
            protein and promotes cell migration. Reduced expression of this
            gene has been observed in human psoriasis patients. Mutations in
            this gene cause a human immunodeficiency syndrome characterized by
            smooth muscle tumors and impaired T-cell function. [provided by
            RefSeq, May 2017].
            
            Transcript Variant: This variant (1) retains an intron in the 5'
            coding region and contains an alternate in-frame exon in the 3'
            coding region compared to variant 2. The encoded isoform (1, also
            known as CARMIL2a) has the same N- and C-termini but is longer
            compared to isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB113647.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA2146236
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000334583.11/ ENSP00000334958.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4462              AB113647.1         18-4479
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..4462
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /note="capping protein regulator and myosin 1 linker 2"
                     /db_xref="GeneID:146206"
                     /db_xref="HGNC:HGNC:27089"
                     /db_xref="MIM:610859"
     exon            1..143
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..4411
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /note="isoform 1 is encoded by transcript variant 1; RGD,
                     leucine-rich repeat, tropomodulin and proline-rich
                     containing protein; leucine rich repeat containing 16C;
                     leucine-rich repeat-containing protein 16C; capping
                     protein, Arp2/3 and myosin-I linker protein 2;
                     F-actin-uncapping protein RLTPR; RGD motif, leucine rich
                     repeats, tropomodulin domain and proline-rich containing"
                     /codon_start=1
                     /product="capping protein, Arp2/3 and myosin-I linker
                     protein 2 isoform 1"
                     /protein_id="NP_001013860.1"
                     /db_xref="CCDS:CCDS45513.1"
                     /db_xref="GeneID:146206"
                     /db_xref="HGNC:HGNC:27089"
                     /db_xref="MIM:610859"
                     /translation="MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVL
                     ALLRWRAYLLHTTCLPLRVDCTFSYLEVQAMALQETPPQVTFELESLRELVLEFPGVA
                     ALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARLERSSPSESTDPCSPCGGFLE
                     TYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSVAALSYNLW
                     FRCLSCVDMKLSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSG
                     LRELSLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAF
                     DSTLTHLDLSGNPGALGASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTVRGCSVGGW
                     MTGRADWRAGRGGLGPPAGVANSLPPQLFAAVSRGCCTSLTHLDASRNVFSRTKSRAA
                     PAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNTHLRDLHLDLSACELRSAG
                     AQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNVRCKETL
                     DDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDA
                     GAKLLAKALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQR
                     SRPELTARAVHQIQACLLRNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEH
                     VELLGCGAGPQGEAAVRQAEDAIQNANFSLSILPILYEAGSSPSHHWQLGQKLEGLLR
                     QVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVLAGSRGLPELLPEQLLQ
                     DAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPAPDGG
                     EPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPE
                     LAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQH
                     HHRPPPGGPQVPPALPQEGNGLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGAT
                     PVPRTLRKKLGTLFAFKKPRSTRGPRTDLETSPGAAPRTRKTTFGDLLRPPTRPSRGE
                     ELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGARPDKRRPLERGETE
                     LAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPISI
                     KSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSP
                     SPCRTSPSPDSLGLPEDPCLGPRNEDGQLRPRPLSAGRRAVSVHEDQLQAPAERPLRL
                     QRSPVLKRRPKLEAPPSPSLGSGLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP"
     exon            144..235
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            236..289
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            290..352
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            353..477
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            478..569
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            570..640
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            641..714
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            715..790
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            791..879
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            880..974
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1061
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1174
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1437
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1542
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1612
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1694
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1791
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1849
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..2022
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2185
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..2287
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2416
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2530
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2691
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2692..2779
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2780..2920
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2921..2987
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            2988..3223
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            3224..3324
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            3325..3685
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            3686..3808
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            3809..3845
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            3846..3917
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            3918..4139
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            4140..4220
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            4221..4298
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     exon            4299..4462
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4444..4449
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /note="hexamer: ATTAAA"
     polyA_site      4462
                     /gene="CARMIL2"
                     /gene_synonym="CARMIL2b; IMD58; LRRC16C; RLTPR"
                     /note="major polyA site"
ORIGIN      
        1 aggtggctgc cgtgcgggtc tgggccccag gcttcctgtg tgcgcgctcg tcctctgctg
       61 tttcccgccg gagctcgttg ggcctccccg gcccgcccgg cccatggccc agacccccga
      121 cggcatctcc tgtgagctcc gaggcgagat caccaggttc ctgtggccca aagaggtgga
      181 gctgctgctg aaaacctggc tacccgggga gggtgctgtg caaaaccatg tcctggcact
      241 gctacgatgg agagcctacc tgctgcacac cacctgcctc ccgctgaggg tggactgcac
      301 gttcagctac ctggaggtcc aggccatggc gctgcaggag acaccccctc aggtcacctt
      361 tgagctggag tccctgcgtg agctggtcct ggagtttcct ggtgtggccg ccctggaaca
      421 gctggcccag cacgtggctg cagccatcaa gaaggtcttc cctcgctcga cccttgggaa
      481 gctattccgg aggcccacac cagcctccat gctggctcgg ctggagagaa gcagcccctc
      541 ggagtccact gacccctgca gcccctgtgg tggcttcttg gagacatacg aggctctgtg
      601 tgactacaat ggcttccctt tccgagagga gattcagtgg gacgtggaca ccatttacca
      661 tcgccagggc tgccgccatt tcagcctggg agacttcagc cacctcggca gtcgggacct
      721 ggccttgagt gtggctgccc tgtcctacaa cctgtggttc cggtgcctct cctgtgtgga
      781 catgaagctg agccttgagg tctcagaaca gattctgcac atgatgagtc agtcatcaca
      841 cctggaggag ctggtgctgg agacctgcag cctgagggga gactttgtcc gacgactggc
      901 ccaggcgctg gcgggacact caagctctgg gctgcgggag ctcagcctcg cggggaacct
      961 gctggatgac cgaggcatga ctgcactcag cagacacctc gagcgttgtc caggagccct
     1021 gaggagactc agcctggccc agacagggtt gacaccgcga ggaatgaggg ctctgggccg
     1081 ggcactggcc accaatgccg ccttcgactc caccctgacc cacctggacc tttctgggaa
     1141 tcctggggcg ctgggggcct ccgaggacag tgggggcctc tatagcttcc tgagccgtcc
     1201 taacgtactg tcgttcctga atctcgcagg caccgacact gccctggaca ctgtgagggg
     1261 gtgctccgtg gggggatgga tgaccggcag ggcggactgg agggcgggac ggggagggct
     1321 cggtcccccc gcgggtgtag ccaacagcct ccccccgcag ctcttcgcag cggtatcccg
     1381 aggctgctgc accagcctta cccacctcga cgcttcgagg aacgtcttct cccgcacgaa
     1441 gtcccgcgcc gcgccggccg cgctgcagct cttcctcagc cgcgcgcgga cgctgcggca
     1501 cctgggcctg gcgggctgca agctgccgcc cgacgcgctc agagcccttt tggatggcct
     1561 cgcgctcaac acgcacctcc gcgacctgca cctggacctc agcgcttgcg agctgcgctc
     1621 ggccggcgcc caggtgatac aagacttagt gtgcgacgca ggcgctgtga gctccctgga
     1681 tctggcggat aacggcttcg gctcagacat ggtgactctg gtgctggcca tcgggagaag
     1741 ccggtccctg agacatgtgg cgcttggaag gaacttcaac gtccggtgca aggagaccct
     1801 ggacgacgtc ctgcaccgga ttgtccagct catgcaggac gacgattgtc ctcttcagtc
     1861 tctgtcggtg gctgagtctc ggctgaagct gggtgccagc gtcctactcc gggccctagc
     1921 caccaatcct aacctgaccg cgctggatat cagcggcaac gccatggggg acgcgggcgc
     1981 caagttgctg gccaaggcgc tgcgggtcaa ctcgaggctc cggtctgtgg tctgggaccg
     2041 gaaccacaca tctgctttgg gtctgctgga cgtggcgcag gcgctggagc agaaccacag
     2101 cctgaaggcc atgcctctgc cactgaacga cgtggcccag gcgcagcgca gccgcccgga
     2161 actgacagca cgtgcagtcc atcagatcca agcctgtctc ttgaggaaca accgcgcaga
     2221 ccctgcctct tctgaccaca cgacccgcct tcagccactt ggtctggtct cagacccctc
     2281 agagcaggaa gtgaatgaat tgtgtcagtc ggtgcaggag catgtggagc tgctgggctg
     2341 tggggctggg ccccagggtg aagccgctgt gcgccaggcc gaggatgcca tccaaaatgc
     2401 caacttctct ctcagcattc tccccattct atatgaagct ggaagctccc caagccatca
     2461 ctggcagctt gggcagaagc tggagggcct tctgagacag gtgggcgagg tctgccgcca
     2521 ggacatccag gacttcactc aggccacact ggacacagca aggagcctct gcccacagat
     2581 gctgcaggga tccagctgga gggagcagct agagggggtc ctggcaggct cgaggggcct
     2641 cccggagctg ctcccagagc agctgctgca agatgccttc actaggctca gggacatgcg
     2701 gctatcaatc acggggacct tggcagagag cattgtggct caggctttgg caggcctgag
     2761 tgcagcccgg gatcagctgg tggagagtct ggctcagcag gcaacagtga caatgccccc
     2821 tgccctacca gcaccggatg gaggtgagcc cagcctcctt gagcctgggg aattggaagg
     2881 tcttttcttc cccgaggaga aggaagagga gaaggagaag gatgacagtc ctccacagaa
     2941 atggcctgag ctcagccacg gtcttcacct ggtccccttc attcacagtg ctgctgagga
     3001 agcggagccg gagcccgagc tggcggctcc gggagaagat gcagagccgc aggcggggcc
     3061 gtccgcgcgc ggctctccga gccctgccgc ccctgggccc ccggccggcc cgctgccccg
     3121 catggacctg ccactggcgg ggcagcccct gcgccatccg acccgggccc ggccgcggcc
     3181 gcgccgccag caccaccacc gcccgccgcc ggggggcccc caggtacccc cagccttgcc
     3241 gcaggaaggg aatgggctca gtgcccgcgt ggacgagggc gtggaggaat tcttctccaa
     3301 aaggctgatc cagcaggatc gcctctgggc ccccgaggag gacccggcca ctgagggggg
     3361 cgccactcct gtcccccgta cactgcgaaa gaagctgggc accctctttg ccttcaagaa
     3421 gcctcgttca acgcggggtc cacggactga tctagagacc agccctgggg cagctccccg
     3481 aacccgaaaa actacatttg gcgacctact gcggccgcca acccgtccca gccgtggtga
     3541 ggagcttggt ggggctgagg gggacaccag cagccctgac cctgccggca ggagccgacc
     3601 tcgctacaca agagatagca aggcctactc gatgatactg ctgcctgccg aggaggaggc
     3661 aacgctgggt gccagacccg acaagcggcg gcccctggag cggggagaaa cagaactggc
     3721 tccatccttt gaacagcggg tacaagtaat gctgcagagg ataggcgtca gccgaggcag
     3781 cgggggtgcc gaaggcaaga ggaagcaaag caaagatggc gagatcaaga aagctggctc
     3841 agatggtgac attatggaca gttccacgga ggcccctccc atctcgatca agtcccgcac
     3901 ccactctgtg tctgctgacc cttcctgcag acctggccca gggagccagg ggcctgagtc
     3961 tgccacctgg aagacactgg ggcagcagtt gaatgcggag ctcaggagcc gtggttgggg
     4021 ccaacaggat ggtccaggcc ctccctcccc tggtcaaagc ccaagtccct gcagaaccag
     4081 cccctcccca gacagcctgg gcctcccaga ggacccttgc ttgggcccca gaaatgaaga
     4141 tggccagctg aggccgaggc ctctctcggc agggcggcga gcagtgtctg tgcatgagga
     4201 ccagctccag gcccctgctg aacggcccct gaggctgcag cgctcccccg tcctcaaacg
     4261 caggccaaaa ctcgaggcac ctccatcccc aagcctagga tctggccttg gaaccgagcc
     4321 tctgccccca cagcccacag agccctccag ccctgagcgg agcccaccct ccccagccac
     4381 agaccaaaga ggcggcggcc ccaatccctg atcctctcct ctcctgccgc atgagattat
     4441 tttattaaaa aactcaaagg aa
//
