LOCUS       NM_017640               5485 bp    mRNA    linear   PRI 17-JUN-2024
DEFINITION  Homo sapiens capping protein regulator and myosin 1 linker 1
            (CARMIL1), transcript variant 1, mRNA.
ACCESSION   NM_017640
VERSION     NM_017640.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ge,Y., Xiao,B., Zhao,R., Li,B., Yang,S., He,K.F., Gu,H.J. and
            Zuo,S.
  TITLE     CARMIL1 regulates liver cancer cell proliferation by activating the
            ERK/mTOR pathway through the TRIM27/p53 axis
  JOURNAL   Int Immunopharmacol 134, 112139 (2024)
   PUBMED   38739978
  REMARK    GeneRIF: CARMIL1 regulates liver cancer cell proliferation by
            activating the ERK/mTOR pathway through the TRIM27/p53 axis.
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Wang,Q., Notay,K., Downey,G.P. and McCulloch,C.A.
  TITLE     The Leucine-Rich Repeat Region of CARMIL1 Regulates IL-1-Mediated
            ERK Activation, MMP Expression, and Collagen Degradation
  JOURNAL   Cell Rep 31 (13), 107781 (2020)
   PUBMED   32610117
  REMARK    GeneRIF: The Leucine-Rich Repeat Region of CARMIL1 Regulates
            IL-1-Mediated ERK Activation, MMP Expression, and Collagen
            Degradation.
REFERENCE   3  (bases 1 to 5485)
  AUTHORS   Johnson,B., McConnell,P., Kozlov,A.G., Mekel,M., Lohman,T.M.,
            Gross,M.L., Amarasinghe,G.K. and Cooper,J.A.
  TITLE     Allosteric Coupling of CARMIL and V-1 Binding to Capping Protein
            Revealed by Hydrogen-Deuterium Exchange
  JOURNAL   Cell Rep 23 (9), 2795-2804 (2018)
   PUBMED   29847807
  REMARK    GeneRIF: V-1 and CARMIL induce changes in both proteins' binding
            sites on the surface of capping proteins, along with a set of
            internal residues.
REFERENCE   4  (bases 1 to 5485)
  AUTHORS   Alazami,A.M., Al-Helale,M., Alhissi,S., Al-Saud,B., Alajlan,H.,
            Monies,D., Shah,Z., Abouelhoda,M., Arnaout,R., Al-Dhekri,H.,
            Al-Numair,N.S., Ghebeh,H., Sheikh,F. and Al-Mousa,H.
  TITLE     Novel CARMIL2 Mutations in Patients with Variable Clinical
            Dermatitis, Infections, and Combined Immunodeficiency
  JOURNAL   Front Immunol 9, 203 (2018)
   PUBMED   29479355
  REMARK    GeneRIF: Study utilized whole exome sequencing and other assays to
            genetically and functionally characterize three consanguineous
            multiplex Saudi families with CARMIL2 mutations, presenting with
            dermatitis, recurrent skin abscesses and chest infections.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5485)
  AUTHORS   Wei,Y., Tejera,P., Wang,Z., Zhang,R., Chen,F., Su,L., Lin,X.,
            Bajwa,E.K., Thompson,B.T. and Christiani,D.C.
  TITLE     A Missense Genetic Variant in LRRC16A/CARMIL1 Improves Acute
            Respiratory Distress Syndrome Survival by Attenuating Platelet
            Count Decline
  JOURNAL   Am J Respir Crit Care Med 195 (10), 1353-1361 (2017)
   PUBMED   27768389
  REMARK    GeneRIF: A novel genetic variant in leucine-rich repeat-containing
            16A (LRRC16A) has been associated with reduced ARDS risk.
REFERENCE   6  (bases 1 to 5485)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   7  (bases 1 to 5485)
  AUTHORS   Benyamin,B., McRae,A.F., Zhu,G., Gordon,S., Henders,A.K.,
            Palotie,A., Peltonen,L., Martin,N.G., Montgomery,G.W.,
            Whitfield,J.B. and Visscher,P.M.
  TITLE     Variants in TF and HFE explain approximately 40% of genetic
            variation in serum-transferrin levels
  JOURNAL   Am J Hum Genet 84 (1), 60-65 (2009)
   PUBMED   19084217
REFERENCE   8  (bases 1 to 5485)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   9  (bases 1 to 5485)
  AUTHORS   Yang,C., Pring,M., Wear,M.A., Huang,M., Cooper,J.A., Svitkina,T.M.
            and Zigmond,S.H.
  TITLE     Mammalian CARMIL inhibits actin filament capping by capping protein
  JOURNAL   Dev Cell 9 (2), 209-221 (2005)
   PUBMED   16054028
REFERENCE   10 (bases 1 to 5485)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL024509.1, AL160037.17 and AL022170.1.
            
            On Nov 23, 2018 this sequence version replaced NM_017640.5.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.188175.1, FJ009082.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000329474.7/ ENSP00000331983.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-462               AL024509.1         42914-43375
            463-560             AL024509.1         48352-48449
            561-611             AL160037.17        33730-33780
            612-671             AL160037.17        40117-40176
            672-793             AL160037.17        49099-49220
            794-891             AL160037.17        63514-63611
            892-962             AL160037.17        63955-64025
            963-1036            AL160037.17        64254-64327
            1037-1112           AL160037.17        79489-79564
            1113-1201           AL160037.17        84785-84873
            1202-1296           AL160037.17        86043-86137
            1297-1383           AL160037.17        95873-95959
            1384-1487           AL160037.17        102098-102201
            1488-1565           AL160037.17        105348-105425
            1566-1642           AL160037.17        105564-105640
            1643-1747           AL160037.17        108727-108831
            1748-1817           AL160037.17        113782-113851
            1818-1899           AL160037.17        123272-123353
            1900-1999           AL160037.17        124123-124222
            2000-2054           AL160037.17        124324-124378
            2055-2227           AL160037.17        129291-129463
            2228-2296           AL160037.17        130963-131031
            2297-2390           AL160037.17        133860-133953
            2391-2489           AL022170.1         6797-6895
            2490-2618           AL022170.1         15857-15985
            2619-2750           AL022170.1         17949-18080
            2751-2926           AL022170.1         28912-29087
            2927-3014           AL022170.1         32011-32098
            3015-3164           AL022170.1         34703-34852
            3165-3231           AL022170.1         58926-58992
            3232-3428           AL022170.1         59245-59441
            3429-3541           AL022170.1         72417-72529
            3542-3974           AL022170.1         78316-78748
            3975-4056           AL022170.1         82814-82895
            4057-4269           AL022170.1         84063-84275
            4270-4401           AL022170.1         88052-88183
            4402-5485           AL022170.1         97449-98532
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p22.2"
     gene            1..5485
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /note="capping protein regulator and myosin 1 linker 1"
                     /db_xref="GeneID:55604"
                     /db_xref="HGNC:HGNC:21581"
                     /db_xref="MIM:609593"
     exon            1..462
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     CDS             423..4538
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine rich repeat containing 16; leucine rich repeat
                     containing 16A; capping protein, Arp2/3, and Myosin-I
                     Linker homolog 1; leucine-rich repeat-containing protein
                     16A; CARMIL homolog; capping protein, Arp2/3 and myosin-I
                     linker homolog 1; capping protein, Arp2/3 and myosin-I
                     linker protein 1; F-actin-uncapping protein LRRC16A;
                     testicular tissue protein Li 107; capping protein
                     regulator and myosin 1 linker protein 1"
                     /codon_start=1
                     /product="F-actin-uncapping protein LRRC16A isoform 1"
                     /protein_id="NP_060110.4"
                     /db_xref="CCDS:CCDS54973.1"
                     /db_xref="GeneID:55604"
                     /db_xref="HGNC:HGNC:21581"
                     /db_xref="MIM:609593"
                     /translation="MTEESSDVPRELIESIKDVIGRKIKISVKKKVKLEVKGDKVENK
                     VLVLTSCRAFLVTARIPTKLELTFSYLEIHGVVCSKSAQMIVETEKCSISMKMASPED
                     VSEVLAHIGTCLRKIFPGLSPVRIMKKVSMEPSERLASLQALWDSQTVAEQGPCGGFS
                     QMYACVCDWLGFSYREEVQWDVDTIYLTQDTRELNLQDFSHLDHRDLIPIIAALEYNQ
                     WFTKLSSKDLKLSTDVCEQILRVVSRSNRLEELVLENAGLRTDFAQKLASALAHNPNS
                     GLHTINLAGNPLEDRGVSSLSIQFAKLPKGLKHLNLSKTSLSPKGVNSLSQSLSANPL
                     TASTLVHLDLSGNVLRGDDLSHMYNFLAQPNAIVHLDLSNTECSLDMVCGALLRGCLQ
                     YLAVLNLSRTVFSHRKGKEVPPSFKQFFSSSLALMHINLSGTKLSPEPLKALLLGLAC
                     NHNLKGVSLDLSNCELRSGGAQVLEGCIAEIHNITSLDISDNGLESDLSTLIVWLSKN
                     RSIQHLALGKNFNNMKSKNLTPVLDNLVQMIQDEESPLQSLSLADSKLKTEVTIIINA
                     LGSNTSLTKVDISGNGMGDMGAKMLAKALQINTKLRTVIWDKNNITAQGFQDIAVAME
                     KNYTLRFMPIPMYDASQALKTNPEKTEDALQKIENYLLRNHETRKYLQEQAYRLQQGI
                     VTSTTQQMIDRICVKVQDHLNSLRNCGGDAIQEDLKSAERLMRDAKNSKTLLPNLYHV
                     GGASWAGASGLLSSPIQETLESMAGEVTRVVDEQLKALLESMVDAAENLCPNVMKKAH
                     IRQDLIHASTEKISIPRTFVKNVLLEQSGIDILNKISEVKLTVASFLSDRIVDEILDA
                     LSHCHHKLADHFSRRGKTLPQQESLEIELAEEKPVKRSIITVEELTEIERLEDLDTCM
                     MTPKSKRKSIHSRMLRPVSRAFEMEFDLDKALEEVPIHIEDPPFPSLRQEKRSSGFIS
                     ELPSEEGKKLEHFTKLRPKRNKKQQPTQAAVCAANIVSQDGEQNGLMGRVDEGVDEFF
                     TKKVTKMDSKKWSTRGSESHELNEGGDEKKKRDSRKSSGFLNLIKSRSKSERPPTILM
                     TEEPSSPKGAVRSPPVDCPRKDTKAAEHNGNSERIEEIKTPDSFEESQGEEIGKVERS
                     DSKSSPQAGRRYGVQVMGSGLLAEMKAKQEKRAACAQKKLGNDAVSQDSSSPALSGVE
                     RSDGGGAVPKLHPGLPENRFGLGTPEKNTKAEPKAEAGSRSRSSSSTPTSPKPLLQSP
                     KPSLAARPVIPQKPRTASRPDDIPDSPSSPKVALLPPVLKKVPSDKERDGQSSPQPSP
                     RTFSQEVSRRSWGQQAQEYQEQKQRSSSKDGHQGSKSNDSGEEAEKEFIFV"
     exon            463..560
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            561..611
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            612..671
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            672..793
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            794..891
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            892..962
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1036
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1112
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1201
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1296
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1383
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1487
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1565
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1642
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1747
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1817
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1899
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..1999
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2054
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2227
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2296
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..2390
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2489
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2618
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2619..2750
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2751..2926
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3014
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3164
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3231
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            3232..3428
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            3429..3541
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            3542..3974
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            3975..4056
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            4057..4269
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            4270..4401
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     exon            4402..5485
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5462..5467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /note="hexamer: AATAAA"
     polyA_site      5485
                     /gene="CARMIL1"
                     /gene_synonym="CARMIL; CARMIL1a; dJ501N12.1; dJ501N12.5;
                     LRRC16; LRRC16A"
                     /note="major polyA site"
ORIGIN      
        1 gccctctccc acgccttccg ctttcaccta gggctgtagg tgcggcgcgg aggctgggcg
       61 ggagctacgc cggcccaagc cccgccgggg accagcgagc cgggaggagg agcaggcgcc
      121 acagccgccc cgcgccccgc gcccgcttgt aatccggtcc gctccttatt cagccgccgg
      181 gaactgcgag gaggcgtcat gtagcagcag cagcaaatcc gcctcgcatt tgcaactctt
      241 tttttttttt ttggtggggc ggggggcgcg cggcaaaatt ctgtctccgc cccccctttt
      301 cttgcccact tccatttgca agctgcatct gcctctctaa aaaaattgag gagttcgggg
      361 aagggcaggg ggccataaat cagagttgga cctgcaataa cccccacacc tacagggcaa
      421 ccatgaccga ggagagctct gacgttccca gggagttgat agaaagcata aaggatgtta
      481 ttggcagaaa gataaaaatt tcagtgaaga agaaagtaaa gttggaagtt aagggagaca
      541 aagttgaaaa caaagtgctg gtccttacat catgccgagc cttccttgta acagcgcgaa
      601 tccccaccaa gctcgagtta accttcagct acttggagat tcatggcgtc gtttgcagca
      661 agtcagctca gatgattgtg gaaactgaga agtgcagcat ttccatgaag atggcgtcac
      721 ccgaggacgt gagtgaggtg ctggctcaca taggcacctg cctgaggaag atatttcctg
      781 gcctctctcc agtgagaatc atgaaaaaag tctccatgga gccatctgag cgcctggcta
      841 gtctccaggc gctgtgggac agccagaccg tggctgagca gggcccctgt ggtggatttt
      901 ctcagatgta tgcctgtgtt tgtgactggc ttggattttc atacagggaa gaagtacaat
      961 gggatgtgga tacaatttat cttacccaag acaccaggga attgaattta caagatttta
     1021 gtcatcttga ccacagggac ctaataccta tcattgctgc tctggaatat aatcagtggt
     1081 tcacaaaact gtcctctaag gatctaaaac tgtccactga tgtctgtgaa cagatcttga
     1141 gggtggtgag taggtccaat cgactggaag aattggtgtt ggaaaatgct ggacttagaa
     1201 cagattttgc acaaaaactg gccagtgctc tagcacataa tcccaactca ggactccaca
     1261 caattaacct tgctggcaac ccactggagg atagaggtgt gtcctcttta agtattcaat
     1321 ttgccaaact cccaaaggga ttaaagcact taaatttatc taaaacctca ttatcaccta
     1381 aaggggtgaa cagcctttct cagtcactca gtgccaatcc attgaccgcc tctacccttg
     1441 tccacctcga cctctcaggg aacgtccttc gtggagatga cctctcacac atgtataatt
     1501 ttttggccca gccaaatgcc attgttcatc tggatttatc caatacagaa tgttccctgg
     1561 acatggtctg tggagctctt ctccgtggat gccttcaata tttagctgtg ctcaacctct
     1621 ccagaactgt cttctctcac cggaaaggaa aagaagtacc tccatctttc aagcaatttt
     1681 ttagtagttc tctggctttg atgcacatca acctttcagg cacaaaactg tctcctgagc
     1741 ccttaaaagc actgttattg ggcctggctt gtaatcataa cttgaaaggg gtttctctgg
     1801 atctcagcaa ctgtgagctg agatcaggag gtgctcaagt attagaaggt tgcattgctg
     1861 aaatacacaa catcaccagc ttagacatct ctgacaatgg tttagaatct gacctatcta
     1921 ccttaatagt gtggctcagt aaaaacagat caatacaaca cctggcgtta ggcaaaaatt
     1981 ttaataatat gaaatccaaa aatctgacac ctgtattgga caacttagta cagatgattc
     2041 aagatgaaga atcacctctg cagtccttgt ccctggctga ctcgaaactc aagactgagg
     2101 tcaccatcat catcaatgcc ctgggaagca acacctccct gaccaaagtg gacattagcg
     2161 gcaacggaat gggggacatg ggagcgaaga tgctggccaa agcactgcag atcaacacta
     2221 agctcaggac tgtaatatgg gacaagaaca acatcactgc acaaggcttt caggatatag
     2281 ctgttgctat ggaaaagaac tacacattaa gatttatgcc aattcctatg tatgatgctt
     2341 ctcaagccct aaaaacaaac cctgaaaaaa cagaagacgc tctgcaaaag atagaaaact
     2401 acctgctacg aaatcacgag actagaaaat accttcaaga gcaggcctac cgcctgcagc
     2461 agggtattgt caccagcacc acccagcaga tgattgacag aatatgtgtg aaagtacaag
     2521 atcatctcaa ctccttacga aattgtgggg gagacgctat ccaggaagat ttaaaatcag
     2581 cagagcggct catgcgtgat gctaagaact ctaaaacgtt gttaccaaat ttataccatg
     2641 ttggtggtgc atcttgggcg ggagccagtg gcttactatc cagtccaatt caggagaccc
     2701 tggaatcaat ggctggagaa gttacaagag tagtagatga acaactaaag gcgttgcttg
     2761 agtccatggt tgatgctgct gagaatcttt gtcccaatgt gatgaaaaaa gcccacattc
     2821 gacaagactt gattcatgcc agcaccgaaa agatttctat tccacgtacc tttgttaaaa
     2881 atgtcctgtt ggagcagtct ggaattgata tccttaacaa aattagtgaa gtaaaattga
     2941 cagtggcctc gttcctgtct gatagaattg tggatgaaat cctggatgca ctctcacatt
     3001 gccatcataa actggctgac catttcagca gacgtggcaa gacccttcct caacaagaat
     3061 ccttagagat cgagctggct gaggagaagc cagttaaacg ttccatcatc acagtggagg
     3121 agctaacaga gatagagcgt ttggaagatc tggatacctg tatgatgacc cctaaatcca
     3181 aaaggaagag tatccatagc cgaatgctgc ggcctgtttc tagggctttt gaaatggagt
     3241 ttgatctaga taaagcgctg gaagaggtac caattcacat cgaagacccg cccttcccat
     3301 ccctcagaca ggagaagcgg agctcgggat ttatctctga gttgccctct gaagagggga
     3361 agaagctgga acactttacc aagttaaggc caaaaaggaa taagaagcag caacccaccc
     3421 aagcagcggt ctgtgctgcc aacatagtct cacaagatgg tgaacagaat ggtctcatgg
     3481 ggagagtgga tgaaggtgta gatgaatttt ttaccaagaa ggtgaccaaa atggattcca
     3541 agaaatggtc aacaagaggc tcagagtccc atgagcttaa tgaaggagga gatgaaaaga
     3601 aaaagcgaga ttctcggaaa agtagtggct ttctcaattt aatcaaatcc cggtccaaat
     3661 ccgagcgacc accaacgatc ttgatgacag aagaaccctc ctcaccaaaa ggggcagtca
     3721 gaagtccacc tgtggactgt cccaggaagg acacaaaggc cgccgagcac aatggcaatt
     3781 ctgaacggat agaggagata aaaacacctg actcctttga agagagtcaa ggggaagaaa
     3841 tagggaaggt ggaacggagt gacagcaaga gcagcccaca ggcagggcgg aggtatgggg
     3901 tccaggtgat gggcagtggt ctgctggcag agatgaaagc caagcaagag aagagagctg
     3961 cgtgtgcgca gaagaagctt gggaatgatg ccgtatccca ggattcttcc agcccagctt
     4021 tgagcggcgt agaacggtcg gatggaggtg gggcagtgcc taaactgcac ccaggtcttc
     4081 cagagaaccg ctttggtttg ggaacaccag aaaagaatac caaagcagaa cccaaagcgg
     4141 aagcaggctc caggtctcgg agctcatcca gcacacctac gagcccgaag cccctcctgc
     4201 agtcccccaa acccagtctg gcagcacggc ccgtcatccc gcagaaacca agaaccgcct
     4261 cacggcctga tgacattcca gactctccat ctagcccgaa agttgccctt cttccacctg
     4321 tcctgaaaaa agttccttca gacaaagaga gagatggcca gagtagcccc cagcccagcc
     4381 ccaggacatt ttcacaggaa gtttcaagga gaagctgggg ccagcaggcc caggagtatc
     4441 aagaacaaaa gcaacggtcc tccagtaaag atggccatca aggcagcaaa tctaatgact
     4501 ccggggaaga agcagaaaaa gagtttattt ttgtgtaaag gtcacccacg cagaagtctt
     4561 cctgtgcagg gtgctttggt agccatcaga gaggaaccaa gggcaacatc ttttcttccc
     4621 aggcgttctt ctctgggtgc tttattctct tctttttctt tatttcgccc ccacccccat
     4681 cccctgcctt tttttttttt tttttttttt ttttttgtat agaaacagat ccatttcttg
     4741 gtaatcaaag cacatttgtt tggtcttcct ccaacccttt gcatttgatt tctaaacatt
     4801 ccttcatatg cctttaatga aagccagcaa ttatcccatg ggccctactt gaatttatct
     4861 gaggcagcta cagattgccc tgcaagatga gtttttggag ataaatgaaa taactggaca
     4921 cacactcaca caagtaacac cacagcagac ctcggagtac tgctaagtgt acctgtgtca
     4981 aatccgcaca ggactcaata tagcaattta ttcttgatgt atgcaattgc acattgtaat
     5041 tatattaaca gagcacacta ataatttgta tagattatat atattagatc ttgggtatgg
     5101 tttttacctt ctcccatggg gaacttcttc cttcctgatg tggaattgta catttaaagc
     5161 ttggtcggtg acctttgcat accatcaacg agcacagcta agaacagagt gagagaggcc
     5221 catggctgat tttaccatgt gcccagatta atgtatatag ttgattggaa tgaggtttta
     5281 tgaatattca tgtttttgaa ggcctttaat ttctgtctgc atattagctt ttaatgtgtg
     5341 attttaagag agaatacttt gacacctgta aaaatcaaaa tactactctt tataagacat
     5401 ttcacaaata ttcacttaca ttacaggctg gaagtatttt attcatatgt atatttatac
     5461 caataaaatg attttacaag tggaa
//
