LOCUS       NM_024744               7974 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens calcium responsive transcription factor (CARF),
            transcript variant 2, mRNA.
ACCESSION   NM_024744 XM_934561
VERSION     NM_024744.17
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Zhang,Y., Meng,H. and Guo,K.
  TITLE     Inhibition of MicroRNA-302c on Stemness of Colon Cancer Stem Cells
            via the CARF/Wnt/beta-Catenin Axis
  JOURNAL   Dig Dis Sci 66 (6), 1906-1915 (2021)
   PUBMED   32617772
  REMARK    GeneRIF: Inhibition of MicroRNA-302c on Stemness of Colon Cancer
            Stem Cells via the CARF/Wnt/beta-Catenin Axis.
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 7974)
  AUTHORS   Bhargava,P., Malik,V., Liu,Y., Ryu,J., Kaul,S.C., Sundar,D. and
            Wadhwa,R.
  TITLE     Molecular Insights Into Withaferin-A-Induced Senescence:
            Bioinformatics and Experimental Evidence to the Role of NFkappaB
            and CARF
  JOURNAL   J Gerontol A Biol Sci Med Sci 74 (2), 183-191 (2019)
   PUBMED   29718136
  REMARK    GeneRIF: Wi-A caused upregulation of CARF (collaborator of ARF)
            demonstrating an activation of DNA damage oxidative stress response
            in both cancer and normal cells. In line with this, upregulation of
            p21WAF1, p16INK4A, and hypophosphorylated pRB and induction of
            senescence were observed demonstrating that Wi-A-induced senescence
            is mediated by multiple pathways in which CARF-mediated DNA damage
            and oxidative stress play a major ro
REFERENCE   4  (bases 1 to 7974)
  AUTHORS   Li,L., Gao,R., Yu,Y., Kaul,Z., Wang,J., Kalra,R.S., Zhang,Z.,
            Kaul,S.C. and Wadhwa,R.
  TITLE     Tumor suppressor activity of miR-451: Identification of CARF as a
            new target
  JOURNAL   Sci Rep 8 (1), 375 (2018)
   PUBMED   29321561
  REMARK    GeneRIF: Data demonstrate that CARF is a new target of miR-451 that
            mediates its tumor suppressor function in normal and stressed
            biological states.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7974)
  AUTHORS   Zheng,F., Zhou,X., Luo,Y., Xiao,H., Wayman,G. and Wang,H.
  TITLE     Regulation of brain-derived neurotrophic factor exon IV
            transcription through calcium responsive elements in cortical
            neurons
  JOURNAL   PLoS One 6 (12), e28441 (2011)
   PUBMED   22174809
REFERENCE   6  (bases 1 to 7974)
  AUTHORS   Cheung,C.T., Hasan,M.K., Widodo,N., Kaul,S.C. and Wadhwa,R.
  TITLE     CARF: an emerging regulator of p53 tumor suppressor and senescence
            pathway
  JOURNAL   Mech Ageing Dev 130 (1-2), 18-23 (2009)
   PUBMED   18555516
  REMARK    Review article
REFERENCE   7  (bases 1 to 7974)
  AUTHORS   Hasan,M.K., Yaguchi,T., Sugihara,T., Kumar,P.K., Taira,K.,
            Reddel,R.R., Kaul,S.C. and Wadhwa,R.
  TITLE     CARF is a novel protein that cooperates with mouse p19ARF (human
            p14ARF) in activating p53
  JOURNAL   J Biol Chem 277 (40), 37765-37770 (2002)
   PUBMED   12154087
REFERENCE   8  (bases 1 to 7974)
  AUTHORS   Tao,X., West,A.E., Chen,W.G., Corfas,G. and Greenberg,M.E.
  TITLE     A calcium-responsive transcription factor, CaRF, that regulates
            neuronal activity-dependent expression of BDNF
  JOURNAL   Neuron 33 (3), 383-395 (2002)
   PUBMED   11832226
  REMARK    GeneRIF: Cloned as a calcium- and neural-selective transcriptional
            activator of the gene for Brain-Derived Neurotrophic Factor (BDNF).
REFERENCE   9  (bases 1 to 7974)
  AUTHORS   Hadano,S., Hand,C.K., Osuga,H., Yanagisawa,Y., Otomo,A.,
            Devon,R.S., Miyamoto,N., Showguchi-Miyata,J., Okada,Y.,
            Singaraja,R., Figlewicz,D.A., Kwiatkowski,T., Hosler,B.A.,
            Sagie,T., Skaug,J., Nasir,J., Brown,R.H. Jr., Scherer,S.W.,
            Rouleau,G.A., Hayden,M.R. and Ikeda,J.E.
  TITLE     A gene encoding a putative GTPase regulator is mutated in familial
            amyotrophic lateral sclerosis 2
  JOURNAL   Nat Genet 29 (2), 166-173 (2001)
   PUBMED   11586298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC021999.1, DA405623.1, AB053309.1 and AC010900.12.
            
            On Nov 22, 2018 this sequence version replaced NM_024744.16.
            
            Transcript Variant: This variant (2) has an alternate splice site
            and an additional exon in the 5' UTR, compared to variant 1.
            Variants 1, 2, 6 and 7 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB053309.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000438828.4/ ENSP00000414644.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                BC021999.1         67-116
            51-202              DA405623.1         40-191
            203-460             DA405623.1         193-450
            461-3301            AB053309.1         1-2841
            3302-7974           AC010900.12        176206-180878
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.2"
     gene            1..7974
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="calcium responsive transcription factor"
                     /db_xref="GeneID:79800"
                     /db_xref="HGNC:HGNC:14435"
                     /db_xref="MIM:607586"
     exon            1..828
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            829..995
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1114
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1235
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     CDS             1158..3335
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="isoform 1 is encoded by transcript variant 2;
                     amyotrophic lateral sclerosis 2 chromosomal region
                     candidate gene 8 protein; calcium-response factor; testis
                     development protein NYD-SP24; amyotrophic lateral
                     sclerosis 2 (juvenile) chromosome region, candidate 8"
                     /codon_start=1
                     /product="calcium-responsive transcription factor isoform
                     1"
                     /protein_id="NP_079020.13"
                     /db_xref="CCDS:CCDS42801.1"
                     /db_xref="GeneID:79800"
                     /db_xref="HGNC:HGNC:14435"
                     /db_xref="MIM:607586"
                     /translation="MEQSNDSLRVNHNDGEESKTSAQVFEHLICMDSRDSSFGQNDSP
                     TVLPITTREANNSLISQNIPGPLTQTQTLSAEQFHLVDQNGQAIQYELQSLGESNAQM
                     MIVASPTENGQVLRVIPPTQTGMAQVIIPQGQLVDVNSPRDVPEEKPSNRNLPTVRVD
                     TLADNTSNYILHPQTSFPLPKKSVTGMLEEPLLGPLQPLSSNTPIWACRLRSCEKIGD
                     SYRGYCVSETELESVLTFHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVN
                     AGSRAVVMECQYGPRRKGFQLKKVSEQESRSCQLYKATCPARIYIKKVQKFPEYRVPT
                     DPKIDKKIIRMEQEKAFNMLKKNLVDAGGVLRWYVQLPTQQAHQYHELETPCLTLSPS
                     PFPVSSLEEEETAVRDENCALPSRLHPQVAHKIQELVSQGIEQVYAVRKQLRKFVERE
                     LFKPDEVPERHNLSFFPTVNDIKNHIHEVQKSLRNGDTVYNSEIIPATLQWTTDSGNI
                     LKETMTVTFAEGNSPGESITTKVETNQTRGSLSPEPTHLLSSLSSFQPKIFTQLQGLQ
                     LQPRYTSPDESPAVVSVNNQPSSSPSGLLDTIGSAVMNNNSLLLGQSHSLQRDTCLTQ
                     NNSTASTMGNLPEPDQNLVAMDELVEVGDVEDTGNLEGTVHRILLGDVQTIPIQIIDN
                     HSALIEENPESTISVSQVKQEPKEPALSMEAKKTVDYKKLSAT"
     exon            1236..1463
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1584
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1714
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1799
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1989
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2110
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2254
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2488
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2651
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2715
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2846
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..3216
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     exon            3217..7974
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6037..6042
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="hexamer: AATACA"
     polyA_site      6053
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="major polyA site"
     regulatory      6176..6181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="hexamer: AGTAAA"
     polyA_site      6196
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="major polyA site"
     regulatory      6662..6667
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="hexamer: ATTAAA"
     polyA_site      6687
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
     regulatory      6754..6759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="hexamer: AATAAA"
     polyA_site      6774
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="major polyA site"
     regulatory      7545..7550
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="hexamer: AATAAA"
     polyA_site      7568
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
     regulatory      7845..7850
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="hexamer: AATAAA"
     polyA_site      7871
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
     regulatory      7945..7950
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
                     /note="hexamer: AATAAA"
     polyA_site      7974
                     /gene="CARF"
                     /gene_synonym="ALS2CR8; NYD-SP24"
ORIGIN      
        1 gcctgcgcat tatgctggtc tccatggcgg ggcctcggag ccaagacgag gttgagtaga
       61 ctcgttttga attttctccc ctctgctccg gcggacttcc catgtcgcct tgtggggcta
      121 tcggcggcgg caggactggg ggagtcagag gtctggcagc gctgtctgcg cagacctacc
      181 ggacgctacc tcccaacccc ccgtcttcct cctgcctcct cctcctcccg tcacctcctg
      241 acccgccgga gctccgagca actgccggcc tccgccccca gccgcagccg gtcactggcg
      301 gcgccttccg cgccaagctt gggggccttt tcggggtccc acatggcacg gcttccgacc
      361 cccggcccgg gacggggctc gcaggcccca gaggggcagg ctggagaagg aggaggttag
      421 gtgtcttcag gagggttgct gagcccaagg acgcgccatc gccgcggaga aggagccgga
      481 ccccttgggc ggagcgccca atgtgtggtc cctcacgccg tcccgcacct tgctttttag
      541 ggttcttttt ccgctttctg agccctttta taccttacgt ttagaagggg aaaatcatcc
      601 tcccacacct tctccccgac tttttgcctt ttttgtcttg aagttaccca aaggcctgtg
      661 tattgttctc aatggtccca agaattactc taatatagtt gtttttctga gggaggatgg
      721 atggagataa ctatcctgat cccaatgtca ctttttaagg cattcgcttc aagagacaag
      781 cagtttagaa tcaggcagaa ctggattgca aaatttatgg gcagaccgag aaaatgctgc
      841 tgtgaagacc caattaaagc tttcacataa atgaagctac agctatcatg atttagtgcc
      901 cccaaaagga agaacttcag tggacaagaa aggacatttc tgggggtagt agaatgcttg
      961 aggcctggaa tttaaacctg agccactatc tgaaggttta atatatcttt gccaagtcca
     1021 agggtacaaa gattttctcc tgtgttttct tctagaagtt ttatcatttg atagtctgtg
     1081 atccatttca agttgatttg ctatctggtg tgagctatta aataatagaa gaaaatggaa
     1141 ttgaatatga tgtcagcatg gaacaatcta atgattcatt aagagtcaac cataatgacg
     1201 gtgaagagtc aaaaaccagt gctcaagtat ttgagcatct aatctgtatg gactccaggg
     1261 attcttcctt tggacaaaat gattctccta cagttttgcc catcactact cgtgaagcaa
     1321 ataattcact catatcacag aatataccag ggcccctgac tcagacacag actctttctg
     1381 cagagcaatt ccatctagtg gaccaaaatg ggcaggctat tcaatatgaa cttcagtcat
     1441 tgggggaatc caatgcacaa atgatgatcg ttgccagccc aacagaaaat ggacaggtac
     1501 ttcgtgtaat tccacctacc cagacaggaa tggcacaagt gattatacct caggggcaac
     1561 ttgtggatgt gaatagtcct cgggatgtcc ctgaagagaa acccagtaac agaaacttac
     1621 caactgtaag agtggatact ctagcagaca ataccagcaa ttacattctt catcctcaaa
     1681 catccttccc attgcccaaa aagtcagtga ccggaatgct ggaagaaccc cttctggggc
     1741 ctcttcagcc actttcttct aatacaccta tatgggcctg ccgtcttagg agctgtgaga
     1801 aaattggaga ttcataccgt ggctactgtg taagtgagac tgaattagaa agtgtcctaa
     1861 catttcacaa gcagcaaaca cagagtgttt gggggacccg tcagtctcca agcccagcca
     1921 agcctgctac acgcttgatg tggaaatccc agtatgttcc atatgatgga atcccatttg
     1981 ttaatgcagg gagtagagct gtggtaatgg agtgtcagta tgggccaaga agaaaaggtt
     2041 tccagttaaa aaaagtcagt gagcaggaaa gcaggtcttg tcagctctac aaagccactt
     2101 gtccagctcg gatttacatt aaaaaggtac agaagtttcc tgaatataga gttcctacag
     2161 accccaaaat tgacaagaaa attatcagaa tggagcagga gaaagctttt aacatgctaa
     2221 agaagaactt ggtagatgct ggtggtgttc ttaggtggta tgtacagtta cctacacagc
     2281 aagctcatca gtatcatgaa ttagagactc cctgcctcac tttgtcacct tctccttttc
     2341 ctgtgtcttc tcttgaagaa gaggaaactg cagttagaga tgagaattgt gcattaccct
     2401 cacgtttaca tcctcaagta gcacataaga ttcaagaatt agtatcacag ggaatagaac
     2461 aagtgtatgc agtaaggaaa cagctaagaa aatttgtgga aagggaactg ttcaaacccg
     2521 atgaggtacc tgaaagacat aatttatctt tttttccaac tgtaaatgat ataaaaaatc
     2581 acatccatga ggtacagaaa tccttgagaa atggagatac ggtatataac tcagagatta
     2641 ttccagcaac gcttcaatgg actacagaca gtgggaatat tctcaaagag accatgacag
     2701 ttacatttgc agaaggaaat tcaccaggag aatcaattac caccaaagtg gaaacaaatc
     2761 agaccagggg ttctttgtct cctgagccaa cccacttgct ctcctcactc tcctcatttc
     2821 agcccaaaat atttacacaa ctacagggtt tgcagttaca accaaggtac acctctcctg
     2881 atgaatcacc agctgtggta tcagtaaata accagccgtc ctctagtcct tcaggacttc
     2941 tggatacaat aggaagtgct gtaatgaata ataattctct actgcttggt caaagtcata
     3001 gccttcaaag agatacatgc ttaacccaaa acaatagtac tgcctccacc atgggtaacc
     3061 ttccagaacc agatcaaaat ctagttgcaa tggacgagct ggtagaagtt ggagatgttg
     3121 aggatacagg gaatctggaa ggaactgttc atcggattct gttgggagat gtgcagacta
     3181 ttccaataca gattatagac aaccactcag ctcttattga agaaaatcca gaaagtacca
     3241 tttctgtgag ccaagttaaa caagaaccca aagaaccagc attgtctatg gaagcaaaaa
     3301 aaactgtgga ctataagaaa ttatctgcta cataaattat tgaagctttt cagaatttac
     3361 aactctggtg acttctgatg tcttagaaag gaaggtgaat atagtcaaag cgtggcattg
     3421 agataattgg actgaagacc agtttgatga gaagctttta tttaaaactg atatatttgt
     3481 tgccagttcc atatttttac cttccttaag agatgtttta ctcttcttat tttgtataat
     3541 ttttatgctc tttgattatc taataaggcc agtatttcaa aagctgttct gaatttatcc
     3601 acagtaatat agtctgaaca caaatagttt acttaactta tccttggaaa tattcaattt
     3661 tggttattac aaaacagaaa agaacaacag caacaaaaac attgtgtgaa tgtatgtttg
     3721 tatgtgtgta tgtatatcat gaattttttt tttaagttct gaaaaagaac ttgttctctc
     3781 cttaaagttg tgaagaaatt gttaattgcc agacaggtaa gaaaatgttt ataatctatc
     3841 tcattaagaa agatgaagta ttagatttta catcataaca caaagtccag cagctacatc
     3901 tgtggaaact gtgcagttgc attttagcct cttcccttcc aatttttgta cttttaacta
     3961 caaaccagta gtatactaat ggttatctaa tagaaattta aagtgtcttg gtaagtactg
     4021 agaattttta cttgaattaa tcagataagc aacagaaatc aacatatgta gcatggattt
     4081 gtgctatatt ggaagtaaaa cactataggc agaaagatgt agaaattaag agaattgaaa
     4141 aagaataata gaattccttg gtgtttacag ataatttgga aaactttttg atgacagcag
     4201 aggatttttt cccccttatt ttgtaatgaa gatccagcac tggttaaaaa ataatttgat
     4261 ttgtagttac atttttctat gccagagctc ttactgaatt gatgatatta ggatttctag
     4321 tattttaagg taagtattta tagtttaaaa tttagtttat atttaaaact ttaaaacatc
     4381 tgatctggga tttttttgtt aagtaagtat attaacttca aataccaaat tactactaag
     4441 cagtatattg attataagca tctgttgtct aaagattggt actaggctgg acatggtggc
     4501 tcacacctgt aatcacaata ctttgggagg ctgaggtggg tagataacct gaggtcagga
     4561 gttcgagacc agcctggcca acgtggtgaa accctgtttc tactaaaaat acaaaaatta
     4621 gctgggtgtg gtggtgggca cctgtaatcc cagctacttg ggaggctaag gcaggagaat
     4681 cgcttgaaca ccggaggcgg aggttgcaat gagccgagat cgcgccattg cactccagcc
     4741 tgggtgacaa gagaaaaact ccatctcaaa aaaaaaaaaa agattggtaa actagaaata
     4801 tcaactataa ttgtcaggaa attaacctaa aggcttttgt ttctccagag accatttgtg
     4861 ggtatatttt atattttaaa aataaagatt ttaaatggat atcaatgttt aatattcaaa
     4921 acatgcacaa tataatatca aatttaacct taatacttta catgaatatg agtcactttt
     4981 ggcttttcta agtccagcct cccattctta attgacttgg tttcaggtac atgacatgta
     5041 tctaaatata aagtactgtt atagtagagg taggtggaat tctaatattc cctgctcttt
     5101 agatttatat tttatttaat ataaagttta ttatttatac cctttttaat gcaaagctaa
     5161 tatttagtta tatgtatgat ttttaataaa aattacttta ctttttccta tgaatttaaa
     5221 acatttgatg ttggcatttt atacaggcag gttgccaaat ttaattatgt tacactaagt
     5281 taaacacagc acttaattac ctcaaatcca gatttctctt aatctgtgtg tacataaaag
     5341 gaataagtcc aaattaattt atagtttagt gttgcactat aggtaatatt cttttgttat
     5401 ttctagtttt tatggaatcc ttttctttca agattctaaa taataaatcg ttacgtctca
     5461 atagtggtaa tagtggtaat ggtttattat tagctgtttc accaattgaa ctacattctt
     5521 ttggagacat tcctttcttt tttgatgttg atttgcaaag gagaaatgct tgttaatata
     5581 caaactctag tcagttacct gtgaggatga gatgtgtcag aggactcaga ctttggtata
     5641 gttagaaaaa taatattttt ctcacttgat tctgtgtgaa tgtctcttct gatactgttt
     5701 tttaatcaga aagttacaga gcaactttcc cacaaacaaa ccaataaaac aaaaccccac
     5761 aaaaaccaag agcatgaaga aaagtttaaa tccctaatta tatactcttt tggtttttag
     5821 gtagaaactg gagctatcaa tattttcatg taagtatttc aaggagtagc tctttaaaaa
     5881 tggtttctaa attagatttt aaaaattcat tgtcatctaa atgcatgttt agtcacaatg
     5941 tatcagtatt gtgtttaaag gtgctcagca tcactttttg aaaactatgt ttacatattt
     6001 aaaatgttta attattttga aatgtatagg gtattaaata caagcttcta aaattttcag
     6061 tatctatatt ggctttctct aacagctcta cagcaaagtt tgacatgttt tttgtgtgtg
     6121 tcatgtattg taagttgtaa tatctgtagg agtgagttgg acattctaaa ttagcagtaa
     6181 aaaattcaca attacagctc agcttgttta ttcattatag tttccacatt tccaacttcc
     6241 aagtgaactt tatatatact acagtgaatt atagataaat acagccatgg tggacctttc
     6301 cctcaatagt gattttttgt acaaacagtt agtataatta aaaggaaaag cttttatatt
     6361 ttatgcaagg taaatgtttc ctaaatgtca caaaagtgat agaaaacata gcttttggga
     6421 tctggtcctg cattagagtt tctaattctg agacccacca gcttgtgttt catcttcttt
     6481 atatcctgtc agaatacata cttaatcatg tgggtatgag aaagatgttg tttaacttgt
     6541 accaggttta aaaaaagagg tttaaaaaat gtctgtgcgt atatatatat atatatatat
     6601 atatatatat atatatatag caacttgatg tatagtgtcc ttgttaccat gtattttttt
     6661 cattaaaaat cctgcttttt ttttatactt gttagttttc tttttatatt tttcttagag
     6721 atagtggcaa ttttgttcat cttgaagatg ctgaataaac acttgaatac ccgagaattt
     6781 tggccagaga tttatgtcca atttggaacc tgaaagtttt aagcataact acaagtaact
     6841 cctgttagca ttacattttg gggagacata tatgcattta ataccctgca actcagccaa
     6901 gcatcaacat tttaaaacaa tgtgtgtaat aagtaattct ccacgcccac ccacccccag
     6961 ccccccgatg ttttggcacc atgacatttt caagtatagc tttatatttt aggagtaatg
     7021 acaaagtatg tgatcaaaag agaagggatt attaattttt tccctctttg ctttttttta
     7081 aactttattc cactctgtac ctatttttga ggaccttgaa aatcttaatc tctccagaag
     7141 ggatactgca ttggttcttc tccatcagct ggcttagaca tcaaacatct taaatgatac
     7201 actgttttaa tatctaaaaa tctgtcattc tacttaatta acttagtcat aaggacaaag
     7261 ccttagtata tttttgccac atacatgtta tgtataaatt ttactgttga ataatacaag
     7321 tattcattgc tgactgaatt cttaaaactt tgggaataga aaaaacacat ttattaatgg
     7381 atgctaaagt gattcctgag aaaaataatg tgtatggtta atatggaaaa cctttggtaa
     7441 tgtgatttgg gtataagaat actaaaacca gagtacaggg tgtgaatcat gtaccaggag
     7501 ggattttatt tttaaatatc tttattgagg tataattgaa atacaataaa ctgtacatat
     7561 ttaaagtacc caatatgata agttttgtca tatgcataca gccatgaaat catcagcata
     7621 agcaaggtaa tgaacatatt catcactctc aaagtttcat agtacctctt tgtgatccct
     7681 tcttacttaa tcctccttgc caccccacca tcaccagacc actgatctgc tttctttatg
     7741 atcagtttgc tacaaatgaa atcacataat atgaaccttt tttggtctag cttttgtcac
     7801 ttagcatagt aattttgtga tttgtctata ttgttgcatt tatgaataaa tcatttatct
     7861 gctgctgagt agaactgcat tttatgaata tatcaatttg tttattcttt tgatgatgga
     7921 cattaatagt tctaggctat tatgaataaa gtttccatga acatctatgt acaa
//
