LOCUS       NM_001184903            5246 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens caspase recruitment domain family member 8 (CARD8),
            transcript variant 5, mRNA.
ACCESSION   NM_001184903
VERSION     NM_001184903.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Karakaya,T., Slaufova,M., Di Filippo,M., Hennig,P., Kundig,T. and
            Beer,H.D.
  TITLE     CARD8: A Novel Inflammasome Sensor with Well-Known
            Anti-Inflammatory and Anti-Apoptotic Activity
  JOURNAL   Cells 13 (12), 1032 (2024)
   PUBMED   38920661
  REMARK    GeneRIF: CARD8: A Novel Inflammasome Sensor with Well-Known
            Anti-Inflammatory and Anti-Apoptotic Activity.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Fransen,K., Hiyoshi,A., Paramel,G.V. and Hurtig-Wennlof,A.
  TITLE     Association between C10X polymorphism in the CARD8 gene and
            inflammatory markers in young healthy individuals in the LBA study
  JOURNAL   BMC Cardiovasc Disord 24 (1), 103 (2024)
   PUBMED   38350853
  REMARK    GeneRIF: Association between C10X polymorphism in the CARD8 gene
            and inflammatory markers in young healthy individuals in the LBA
            study.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5246)
  AUTHORS   Lee,Y.H. and Song,G.G.
  TITLE     The role of NLRP3 and CARD8 polymorphisms in the risk of rheumatoid
            arthritis: A meta-analysis of genetic association studies
  JOURNAL   Int J Rheum Dis 26 (11), 2214-2222 (2023)
   PUBMED   37712526
  REMARK    GeneRIF: The role of NLRP3 and CARD8 polymorphisms in the risk of
            rheumatoid arthritis: A meta-analysis of genetic association
            studies.
            Review article
REFERENCE   4  (bases 1 to 5246)
  AUTHORS   Agostini,L., Martinon,F., Burns,K., McDermott,M.F., Hawkins,P.N.
            and Tschopp,J.
  TITLE     NALP3 forms an IL-1beta-processing inflammasome with increased
            activity in Muckle-Wells autoinflammatory disorder
  JOURNAL   Immunity 20 (3), 319-325 (2004)
   PUBMED   15030775
REFERENCE   5  (bases 1 to 5246)
  AUTHORS   Stilo,R., Leonardi,A., Formisano,L., Di Jeso,B., Vito,P. and
            Liguoro,D.
  TITLE     TUCAN/CARDINAL and DRAL participate in a common pathway for
            modulation of NF-kappaB activation
  JOURNAL   FEBS Lett 521 (1-3), 165-169 (2002)
   PUBMED   12067710
  REMARK    GeneRIF: TUCAN/CARDINAL and DRAL participate in a common pathway
            for modulation of NF-kappaB activation.
REFERENCE   6  (bases 1 to 5246)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Fariss,R.N., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human RPE/choroid for the
            NEIBank Project: over 6000 non-redundant transcripts, novel genes
            and splice variants
  JOURNAL   Mol Vis 8, 205-220 (2002)
   PUBMED   12107410
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5246)
  AUTHORS   Zhang,H. and Fu,W.
  TITLE     NDPP1 is a novel CARD domain containing protein which can inhibit
            apoptosis and suppress NF-kappaB activation
  JOURNAL   Int J Oncol 20 (5), 1035-1040 (2002)
   PUBMED   11956601
  REMARK    GeneRIF: Expression and characterization of NDPP1
REFERENCE   8  (bases 1 to 5246)
  AUTHORS   Razmara,M., Srinivasula,S.M., Wang,L., Poyet,J.L., Geddes,B.J.,
            DiStefano,P.S., Bertin,J. and Alnemri,E.S.
  TITLE     CARD-8 protein, a new CARD family member that regulates caspase-1
            activation and apoptosis
  JOURNAL   J Biol Chem 277 (16), 13952-13958 (2002)
   PUBMED   11821383
  REMARK    GeneRIF: CARD-8 protein, a new CARD family member that regulates
            caspase-1 activation and apoptosis. (card-8 protein)
REFERENCE   9  (bases 1 to 5246)
  AUTHORS   Bouchier-Hayes,L., Conroy,H., Egan,H., Adrain,C., Creagh,E.M.,
            MacFarlane,M. and Martin,S.J.
  TITLE     CARDINAL, a novel caspase recruitment domain protein, is an
            inhibitor of multiple NF-kappa B activation pathways
  JOURNAL   J Biol Chem 276 (47), 44069-44077 (2001)
   PUBMED   11551959
REFERENCE   10 (bases 1 to 5246)
  AUTHORS   Pathan,N., Marusawa,H., Krajewska,M., Matsuzawa,S., Kim,H.,
            Okada,K., Torii,S., Kitada,S., Krajewski,S., Welsh,K., Pio,F.,
            Godzik,A. and Reed,J.C.
  TITLE     TUCAN, an antiapoptotic caspase-associated recruitment domain
            family protein overexpressed in cancer
  JOURNAL   J Biol Chem 276 (34), 32220-32229 (2001)
   PUBMED   11408476
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC046136.1, AK297045.1,
            AF322184.1, AC011466.6 and CA448628.1.
            
            Summary: The protein encoded by this gene belongs to the caspase
            recruitment domain (CARD)-containing family of proteins, which are
            involved in pathways leading to activation of caspases or nuclear
            factor kappa-B (NFKB). This protein may be a component of the
            inflammasome, a protein complex that plays a role in the activation
            of proinflammatory caspases. It is thought that this protein acts
            as an adaptor molecule that negatively regulates NFKB activation,
            CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in
            this gene may be associated with a susceptibility to rheumatoid
            arthritis. Alternatively spliced transcript variants have been
            described for this gene. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (5) differs in the 5' UTR and
            lacks an alternate exon in the 3' coding region, which results in a
            frameshift compared to variant 1. This results in a shorter protein
            (isoform c), compared to isoform a. Variants 4 and 5 encode the
            same isoform (c).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.39850.1, AK297045.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                BC046136.1         103-138
            37-385              AK297045.1         23-371
            386-386             AF322184.1         285-285
            387-1425            AK297045.1         373-1411
            1426-4790           AC011466.6         126122-129486       c
            4791-5203           CA448628.1         51-463              c
            5204-5246           CA448628.1         6-48                c
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..5246
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="caspase recruitment domain family member 8"
                     /db_xref="GeneID:22900"
                     /db_xref="HGNC:HGNC:17057"
                     /db_xref="MIM:609051"
     exon            1..97
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            98..199
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..1319
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="isoform c is encoded by transcript variant 5; tumor
                     up-regulated CARD-containing antagonist of caspase nine;
                     CARD inhibitor of NF-kappaB-activating ligands; apoptotic
                     protein NDPP1; tumor up-regulated CARD-containing
                     antagonist of CASP9"
                     /codon_start=1
                     /product="caspase recruitment domain-containing protein 8
                     isoform c"
                     /protein_id="NP_001171832.1"
                     /db_xref="CCDS:CCDS54288.1"
                     /db_xref="GeneID:22900"
                     /db_xref="HGNC:HGNC:17057"
                     /db_xref="MIM:609051"
                     /translation="MEKKECPEKSSSSEEELPRRDSGSSRNIDASKLIRLQGSRKLLV
                     DNSIRELQYTKTGIFFQAEACVTNDTVYRELPCVSETLCDISHFFQEDDETEAEPLLF
                     RAVPECQLSGGDIPSVSEEQESSEGQDSGDICSEENQIVSSYASKVCFEIEEDYKNRQ
                     FLGPEGNVDVELIDKSTNRYSVWFPTAGWYLWSATGLGFLVRDEVTVTIAFGSWSQHL
                     ALDLQHHEQWLVGGPLFDVTAEPEEAVAEIHLPHFISLQAGEVDVSWFLVAHFKNEGM
                     VLEHPARVEPFYAVLESPSFSLMGILLRIASGTRLSIPITSNTLIYYHPHPEDIKFHL
                     YLVPSDALLTKAIDDEEDRFHGVRLQTSPPMEPLNFGSSYIVSNSANLKVMPKWISSL
                     "
     exon            200..349
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            350..490
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            491..531
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            532..682
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            683..912
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1175
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1301
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1346
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..5236
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2514..2519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="hexamer: AACAAG"
     polyA_site      2535
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="major polyA site"
     regulatory      3644..3649
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="hexamer: AATAAA"
     polyA_site      3672
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
     polyA_site      4902
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
     regulatory      5044..5049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="hexamer: CATAAA"
     polyA_site      5067
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
     regulatory      5194..5199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="hexamer: AATAAA"
     polyA_site      5236
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
ORIGIN      
        1 tgaccaagag gaacagaaca cagagcagcc tggcagtgtc caagcaacaa gcctccgctc
       61 ctccttcctg caccctgggg ctcctgaaac tcacatgggt aaaaaagata cagtaaagac
      121 ataaatacca catttgacaa atggaaaaaa aggagtgtcc agaaaagagt agcagcagtg
      181 aggaagagct gccgagacgg gacagtggat ccagtaggaa catagatgca tccaaactca
      241 ttagactaca aggatcacgg aaactgttgg ttgacaatag catacgggaa ctgcaataca
      301 caaaaactgg aatttttttt caggctgagg cctgtgtgac aaatgatacg gtatacaggg
      361 agctaccctg tgtttctgag accctttgtg acatctcaca ttttttccaa gaagatgatg
      421 agacagaggc agagccatta ttgttccgtg ctgttcctga gtgtcaacta tctggggggg
      481 acattcccag tgtatcagaa gagcaggaat cttcagaggg acaagattca ggagacattt
      541 gctcagaaga gaatcaaata gtttcctctt atgcttctaa agtctgtttt gagatcgaag
      601 aagattataa aaatcgtcag tttctggggc ctgaaggaaa tgtggatgtt gagttgattg
      661 ataagagcac aaacagatac agcgtttggt tccccactgc tggctggtat ctgtggtcag
      721 ccacaggcct cggcttcctg gtaagggatg aggtcacagt gacgattgcg tttggttcct
      781 ggagtcagca cctggccctg gacctgcagc accatgaaca gtggctggtg ggcggcccct
      841 tgtttgatgt cactgcagag ccagaggagg ctgtcgccga aatccacctc ccccacttca
      901 tctccctcca agcaggtgag gtggacgtct cctggtttct cgttgcccat tttaagaatg
      961 aagggatggt cctggagcat ccagcccggg tggagccttt ctatgctgtc ctggaaagcc
     1021 ccagcttctc tctgatgggc atcctgctgc ggatcgccag tgggactcgc ctctccatcc
     1081 ccatcacttc caacacattg atctattatc acccccaccc cgaagatatt aagttccact
     1141 tgtaccttgt ccccagcgac gccttgctaa caaaggcgat agatgatgag gaagatcgct
     1201 tccatggtgt gcgcctgcag acttcgcccc caatggaacc cctgaacttt ggttccagtt
     1261 atattgtgtc taattctgct aacctgaaag taatgcccaa gtggatctcc agcttgtagc
     1321 tgcatcagcc cctcctcctt tctcaggtgc agcctttgtg aaggagaacc accggcaact
     1381 ccaagccagg atgggggacc tgaaaggggt gctcgatgat ctccaagaca atgaggttct
     1441 tactgagaat gagaaggagc tggtggagca ggaaaagaca cggcagagca agaatgaggc
     1501 cttgctgagc atggtggaga agaaagggga cctggccctg gacgtgctct tcagaagcat
     1561 tagtgaaagg gacccttacc tcgtgtccta tcttagacag cagaatttgt aaaatgagtc
     1621 agttaggtag tctggaagag agaatccagc gttctcattg gaaatggata aacagaaatg
     1681 tgatcattga tttcagtgtt caagacagaa gaagactggg taacatctat cacacaggct
     1741 ttcaggacag acttgtaacc tggcatgtac ctattgactg tatcctcatg cattttcctc
     1801 aagaatgtct gaagaaggta gtaatattcc ttttaaattt tttccaacca ttgcttgata
     1861 tatcactatt ttatccattg acatgattct tgaagaccca ggataaagga catccggata
     1921 ggtgtgttta tgaaggatgg ggcctggaaa ggcaactttt cctgattaat gtgaaaaata
     1981 attcctatgg acactccgtt tgaagtatca ccttctcata actaaaagca gaaaagctaa
     2041 caaaagcttc tcagctgagg acactcaagg catacatgat gacagtcttt tttttttttg
     2101 tatgttagga ctttaacact ttatctatgg ctactgttat tagaacaatg taaatgtatt
     2161 tgctgaaaga gagcacaaaa atgggagaaa atgcaaacat gagcagaaaa tattttccca
     2221 ctggtgtgta gcctgctaca aggagttgtt gggttaaatg ttcatggtca actccaagga
     2281 atactgagat gaaatgtggt aaatcaactc cacagaacca ccaaaaagaa aatgagggta
     2341 attcagctta ttctgagaca gacattcctg gcaatgtacc atacaaaaaa taagccaact
     2401 ctgacatttg gattctacca tagactctgt cattttgtag ccatttcagc tgtcttttga
     2461 ttaatgtttt cgtggcacac atatttccat ccttttatgt ttaatctgtt taaaacaagt
     2521 tcctagtaga caccatctgg ttgagtcagt tttttttatg gtgtattttg aacccattct
     2581 gatagtctct tttaactgga agatttcaat tacttacgtt aatgtaatta ttaatatgtt
     2641 aggatttatc ctcagtcagc cagtttgtta tgtcttttct attctactgt tatcacattt
     2701 gtaccactta aagtggaatc taggcacttt atcaccattt agatcctatt accttttctc
     2761 atctaggata tagttatctt ctacataatc tttctgtatc ttaaaaccca tcaataaatt
     2821 attatatatt ttctactttt aatcactcag aagatttaaa aaactcatga gaagagtaat
     2881 ctgttatgtt tttccagata tttaccattt ctgttgctct tccttcatta ttttccaaat
     2941 ttcgttctgc aaatttccac ttcctctgat agacgttttt tagttctttt agagtggttc
     3001 tgataggtac agattctctt attttttgct tcctctgagg acatcttttt ctcaccttca
     3061 ttctcagtga tgttttttgc ttgtagtatt tttagttgac attgttttct gttcagcagt
     3121 ttccttttag cttccgtatt tcctgatgag aaatctgcag tcattcaaat tgttgtttcc
     3181 ctgtatgtag tgtgtcattt ttctgtcaga tttcaaggta tttatcttta gtttttagcc
     3241 atttcattat gttggggatg agtttccttg ttttattccc tttggaattt gctccaattc
     3301 ataaatttgc agttttatgt cttttaccaa acttagaggt tttcagccta atttctaaaa
     3361 atacttttta ttagcctgat tttcatcttt ataggaaata gtttaagtga tgacaagttc
     3421 caatagctta tatgcccaga aggccttcaa aataagaatt ttgaaagaat acagaaaaca
     3481 aacttttata tccttctcat gtcttctact gtaaaattca tatgctttgc tactctaaac
     3541 ctagtttgaa atcaacagtc ttgagaatag atgaaaattt tgatgaatag tggaattctt
     3601 ttaaatggaa acctcttaca tgtgattttc cttgccatct agaaataaac catagtattt
     3661 atgttgaatc aatcaatatt atattttgtt tttttcctcc tcttctgaga ctcttattgt
     3721 ggaaatgtta gacttttatg ttttcctaaa tgtccctgat attctactta tttagaacat
     3781 cttttcattt tttccattat tctgattggg taattttaat ttgtctattt tcaaatttgc
     3841 tggagtgttc acctgttgtt atctgtgtcg tcccactgag tgcattcacc accttttaaa
     3901 ttttggtcac tgtatgtatc agttctaaaa tttccatttt gttctctata ttttaaattt
     3961 cttgccttat attctatttt cctgcaaatg tgtcagcatt tgcttgtttg agcttttttt
     4021 ttttcaagac agggtctcaa ctctgttacc caggctggag tgcagtggtg cgatctcagc
     4081 tcactgcaac ctctgcctcc tggttcaagc gattattgtg cctcagcctc cagagtagct
     4141 gggattacag gcatgcacca ccacagccca gctaattttt tgtattttta gtagagacag
     4201 agttttgcta tgttggccag gctggttttg aactcctggc ctcaagtgat ccacccacct
     4261 cagcctccca aagtgctggg attacaggcc actacacctg gcacatttga gtattttttt
     4321 tttttttttt ttttttttga gatggagtct cgctctgtca tctaggctgg agtgcagtgg
     4381 tgtgatctca gctcactgca gcctctgtct cccgggctca agcgattctc ttgcctcagc
     4441 ctcctgagta gctaggacta caggtgcatg ccaacacgcc cggctaattt ttttttaaaa
     4501 atatttttag tagagacagg gtttcaccat tttggccagg atggtcttga tctcctgacc
     4561 tcatgatcca cccgcctcgg ccttccaaag tgctgggatt acaggcatga gccaccgtgc
     4621 ctggcctcat ttgagtattt ttataatgtc tcttttaaag tctttgtcag ataatttcac
     4681 tgtacatgtt attcagtgtt tggtgtccac tgagttgtca tttgccagac aagtggagat
     4741 ttttgcagct catccttgta ttctcagtag ttccgatatg taccctcgaa atgtgaatgt
     4801 tatcttatga gactctgttt tatttgtatc caacagaaga tgtttattat ttatttggct
     4861 ttctgtgaac tgaggtctta atatcagctc attttaaaag tctttgcagt ggtattcgga
     4921 tctatcctgt gtgtgcctat gagattgggt gcagtgtatc ctgttagctc cattctcagg
     4981 gcgtttgaat gtgaattagg accagcgcaa tgaatgctca agttggggtt gggcgttaga
     5041 attcataaaa gtctttatat gctcagattc tgcagctcca tcctcttcat gattgtgatc
     5101 gtaaggacag tggaattttg cagttgtcta taccttatct tgaagtagaa ctaaattgtt
     5161 acatttgaag aatgtacttc ataaatttac aaaaataaaa aaataggtta atgttattaa
     5221 aacaaaaagg cacacaaaaa aaaaaa
//
