LOCUS       NM_001184900            5610 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens caspase recruitment domain family member 8 (CARD8),
            transcript variant 1, mRNA.
ACCESSION   NM_001184900
VERSION     NM_001184900.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Karakaya,T., Slaufova,M., Di Filippo,M., Hennig,P., Kundig,T. and
            Beer,H.D.
  TITLE     CARD8: A Novel Inflammasome Sensor with Well-Known
            Anti-Inflammatory and Anti-Apoptotic Activity
  JOURNAL   Cells 13 (12), 1032 (2024)
   PUBMED   38920661
  REMARK    GeneRIF: CARD8: A Novel Inflammasome Sensor with Well-Known
            Anti-Inflammatory and Anti-Apoptotic Activity.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Fransen,K., Hiyoshi,A., Paramel,G.V. and Hurtig-Wennlof,A.
  TITLE     Association between C10X polymorphism in the CARD8 gene and
            inflammatory markers in young healthy individuals in the LBA study
  JOURNAL   BMC Cardiovasc Disord 24 (1), 103 (2024)
   PUBMED   38350853
  REMARK    GeneRIF: Association between C10X polymorphism in the CARD8 gene
            and inflammatory markers in young healthy individuals in the LBA
            study.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5610)
  AUTHORS   Lee,Y.H. and Song,G.G.
  TITLE     The role of NLRP3 and CARD8 polymorphisms in the risk of rheumatoid
            arthritis: A meta-analysis of genetic association studies
  JOURNAL   Int J Rheum Dis 26 (11), 2214-2222 (2023)
   PUBMED   37712526
  REMARK    GeneRIF: The role of NLRP3 and CARD8 polymorphisms in the risk of
            rheumatoid arthritis: A meta-analysis of genetic association
            studies.
            Review article
REFERENCE   4  (bases 1 to 5610)
  AUTHORS   Agostini,L., Martinon,F., Burns,K., McDermott,M.F., Hawkins,P.N.
            and Tschopp,J.
  TITLE     NALP3 forms an IL-1beta-processing inflammasome with increased
            activity in Muckle-Wells autoinflammatory disorder
  JOURNAL   Immunity 20 (3), 319-325 (2004)
   PUBMED   15030775
REFERENCE   5  (bases 1 to 5610)
  AUTHORS   Stilo,R., Leonardi,A., Formisano,L., Di Jeso,B., Vito,P. and
            Liguoro,D.
  TITLE     TUCAN/CARDINAL and DRAL participate in a common pathway for
            modulation of NF-kappaB activation
  JOURNAL   FEBS Lett 521 (1-3), 165-169 (2002)
   PUBMED   12067710
  REMARK    GeneRIF: TUCAN/CARDINAL and DRAL participate in a common pathway
            for modulation of NF-kappaB activation.
REFERENCE   6  (bases 1 to 5610)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Fariss,R.N., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human RPE/choroid for the
            NEIBank Project: over 6000 non-redundant transcripts, novel genes
            and splice variants
  JOURNAL   Mol Vis 8, 205-220 (2002)
   PUBMED   12107410
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5610)
  AUTHORS   Zhang,H. and Fu,W.
  TITLE     NDPP1 is a novel CARD domain containing protein which can inhibit
            apoptosis and suppress NF-kappaB activation
  JOURNAL   Int J Oncol 20 (5), 1035-1040 (2002)
   PUBMED   11956601
  REMARK    GeneRIF: Expression and characterization of NDPP1
REFERENCE   8  (bases 1 to 5610)
  AUTHORS   Razmara,M., Srinivasula,S.M., Wang,L., Poyet,J.L., Geddes,B.J.,
            DiStefano,P.S., Bertin,J. and Alnemri,E.S.
  TITLE     CARD-8 protein, a new CARD family member that regulates caspase-1
            activation and apoptosis
  JOURNAL   J Biol Chem 277 (16), 13952-13958 (2002)
   PUBMED   11821383
  REMARK    GeneRIF: CARD-8 protein, a new CARD family member that regulates
            caspase-1 activation and apoptosis. (card-8 protein)
REFERENCE   9  (bases 1 to 5610)
  AUTHORS   Bouchier-Hayes,L., Conroy,H., Egan,H., Adrain,C., Creagh,E.M.,
            MacFarlane,M. and Martin,S.J.
  TITLE     CARDINAL, a novel caspase recruitment domain protein, is an
            inhibitor of multiple NF-kappa B activation pathways
  JOURNAL   J Biol Chem 276 (47), 44069-44077 (2001)
   PUBMED   11551959
REFERENCE   10 (bases 1 to 5610)
  AUTHORS   Pathan,N., Marusawa,H., Krajewska,M., Matsuzawa,S., Kim,H.,
            Okada,K., Torii,S., Kitada,S., Krajewski,S., Welsh,K., Pio,F.,
            Godzik,A. and Reed,J.C.
  TITLE     TUCAN, an antiapoptotic caspase-associated recruitment domain
            family protein overexpressed in cancer
  JOURNAL   J Biol Chem 276 (34), 32220-32229 (2001)
   PUBMED   11408476
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC404273.1, CB119078.1,
            BC056891.1, EU118120.1, AC011466.6 and CA448628.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001184900.2.
            
            Summary: The protein encoded by this gene belongs to the caspase
            recruitment domain (CARD)-containing family of proteins, which are
            involved in pathways leading to activation of caspases or nuclear
            factor kappa-B (NFKB). This protein may be a component of the
            inflammasome, a protein complex that plays a role in the activation
            of proinflammatory caspases. It is thought that this protein acts
            as an adaptor molecule that negatively regulates NFKB activation,
            CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in
            this gene may be associated with a susceptibility to rheumatoid
            arthritis. Alternatively spliced transcript variants have been
            described for this gene. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a, also referred to as T60). Variants 1 and 10 encode the same
            protein (isoform a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.176911.1,
                                           SRR1660803.168838.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000651546.1/ ENSP00000499211.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                DC404273.1         35-120
            87-130              CB119078.1         73-116
            131-329             BC056891.1         105-303
            330-622             EU118120.1         1-293
            623-693             BC056891.1         597-667
            694-1939            EU118120.1         365-1610
            1940-5164           AC011466.6         126122-129346       c
            5165-5570           CA448628.1         58-463              c
            5571-5610           CA448628.1         16-55               c
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..5610
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="caspase recruitment domain family member 8"
                     /db_xref="GeneID:22900"
                     /db_xref="HGNC:HGNC:17057"
                     /db_xref="MIM:609051"
     exon            1..121
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            122..218
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            219..329
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            330..431
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     CDS             373..1986
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="isoform a is encoded by transcript variant 1; tumor
                     up-regulated CARD-containing antagonist of caspase nine;
                     CARD inhibitor of NF-kappaB-activating ligands; apoptotic
                     protein NDPP1; tumor up-regulated CARD-containing
                     antagonist of CASP9"
                     /codon_start=1
                     /product="caspase recruitment domain-containing protein 8
                     isoform a"
                     /protein_id="NP_001171829.1"
                     /db_xref="CCDS:CCDS54289.1"
                     /db_xref="GeneID:22900"
                     /db_xref="HGNC:HGNC:17057"
                     /db_xref="MIM:609051"
                     /translation="MEKKECPEKSSSSEEELPRRDSGSSRNIDASKLIRLQGSRKLLV
                     DNSIRELQYTKTGIFFQAEACVTNDTVYRELPCVSETLCDISHFFQEDDETEAEPLLF
                     RAVPECQLSGGDIPSVSEEQESSEGQDSGDICSEENQIVSSYASKVCFEIEEDYKNRQ
                     FLGPEGNVDVELIDKSTNRYSVWFPTAGWYLWSATGLGFLVRDEVTVTIAFGSWSQHL
                     ALDLQHHEQWLVGGPLFDVTAEPEEAVAEIHLPHFISLQAGEVDVSWFLVAHFKNEGM
                     VLEHPARVEPFYAVLESPSFSLMGILLRIASGTRLSIPITSNTLIYYHPHPEDIKFHL
                     YLVPSDALLTKAIDDEEDRFHGVRLQTSPPMEPLNFGSSYIVSNSANLKVMPKELKLS
                     YRSPGEIQHFSKFYAGQMKEPIQLEITEKRHGTLVWDTEVKPVDLQLVAASAPPPFSG
                     AAFVKENHRQLQARMGDLKGVLDDLQDNEVLTENEKELVEQEKTRQSKNEALLSMVEK
                     KGDLALDVLFRSISERDPYLVSYLRQQNL"
     exon            432..581
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            582..722
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            723..763
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            764..914
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1144
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1407
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1533
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1675
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1720
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..5610
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2888..2893
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="hexamer: AACAAG"
     polyA_site      2909
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="major polyA site"
     regulatory      4018..4023
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="hexamer: AATAAA"
     polyA_site      4046
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
     polyA_site      5276
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
     regulatory      5418..5423
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="hexamer: CATAAA"
     polyA_site      5441
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
     regulatory      5568..5573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
                     /note="hexamer: AATAAA"
     polyA_site      5610
                     /gene="CARD8"
                     /gene_synonym="CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN"
ORIGIN      
        1 cctctgcgag cgttatttca aaagaagttg agaaccagag aaaccgacct aaggggattc
       61 tcccatttgg cccgtcctac cctaaagtca ccacctgctg cttttctgga gcgcttacca
      121 gtgaccaaga ggaacagaac acagagcagc ctggcagtgt ccaagcaaca agcctccgct
      181 cctccttcct gcaccctggg gctcctgaaa ctcacatgag aaggagggct gtctgagatt
      241 cgagggaaac aagctctcag gacttccggt cgccatgatg gctgtgggcg gtaaacgcgg
      301 ttagtgcaag catctgggcc atcttcaatg gtaaaaaaga tacagtaaag acataaatac
      361 cacatttgac aaatggaaaa aaaggagtgt ccagaaaaga gtagcagcag tgaggaagag
      421 ctgccgagac gggacagtgg atccagtagg aacatagatg catccaaact cattagacta
      481 caaggatcac ggaaactgtt ggttgacaat agcatacggg aactgcaata cacaaaaact
      541 ggaatttttt ttcaggctga ggcctgtgtg acaaatgata cggtatacag ggagctaccc
      601 tgtgtttctg agaccctttg tgacatctca cattttttcc aagaagatga tgagacagag
      661 gcagagccat tattgttccg tgctgttcct gagtgtcaac tatctggggg ggacattccc
      721 agtgtatcag aagagcagga atcttcagag ggacaagatt caggagacat ttgctcagaa
      781 gagaatcaaa tagtttcctc ttatgcttct aaagtctgtt ttgagatcga agaagattat
      841 aaaaatcgtc agtttctggg gcctgaagga aatgtggatg ttgagttgat tgataagagc
      901 acaaacagat acagcgtttg gttccccact gctggctggt atctgtggtc agccacaggc
      961 ctcggcttcc tggtaaggga tgaggtcaca gtgacgattg cgtttggttc ctggagtcag
     1021 cacctggccc tggacctgca gcaccatgaa cagtggctgg tgggcggccc cttgtttgat
     1081 gtcactgcag agccagagga ggctgtcgcc gaaatccacc tcccccactt catctccctc
     1141 caagcaggtg aggtggacgt ctcctggttt ctcgttgccc attttaagaa tgaagggatg
     1201 gtcctggagc atccagcccg ggtggagcct ttctatgctg tcctggaaag ccccagcttc
     1261 tctctgatgg gcatcctgct gcggatcgcc agtgggactc gcctctccat ccccatcact
     1321 tccaacacat tgatctatta tcacccccac cccgaagata ttaagttcca cttgtacctt
     1381 gtccccagcg acgccttgct aacaaaggcg atagatgatg aggaagatcg cttccatggt
     1441 gtgcgcctgc agacttcgcc cccaatggaa cccctgaact ttggttccag ttatattgtg
     1501 tctaattctg ctaacctgaa agtaatgccc aaggagttga aattgtccta caggagccct
     1561 ggagaaattc agcacttctc aaaattctat gctgggcaga tgaaggaacc cattcaactt
     1621 gagattactg aaaaaagaca tgggactttg gtgtgggata ctgaggtgaa gccagtggat
     1681 ctccagcttg tagctgcatc agcccctcct cctttctcag gtgcagcctt tgtgaaggag
     1741 aaccaccggc aactccaagc caggatgggg gacctgaaag gggtgctcga tgatctccaa
     1801 gacaatgagg ttcttactga gaatgagaag gagctggtgg agcaggaaaa gacacggcag
     1861 agcaagaatg aggccttgct gagcatggtg gagaagaaag gggacctggc cctggacgtg
     1921 ctcttcagaa gcattagtga aagggaccct tacctcgtgt cctatcttag acagcagaat
     1981 ttgtaaaatg agtcagttag gtagtctgga agagagaatc cagcgttctc attggaaatg
     2041 gataaacaga aatgtgatca ttgatttcag tgttcaagac agaagaagac tgggtaacat
     2101 ctatcacaca ggctttcagg acagacttgt aacctggcat gtacctattg actgtatcct
     2161 catgcatttt cctcaagaat gtctgaagaa ggtagtaata ttccttttaa attttttcca
     2221 accattgctt gatatatcac tattttatcc attgacatga ttcttgaaga cccaggataa
     2281 aggacatccg gataggtgtg tttatgaagg atggggcctg gaaaggcaac ttttcctgat
     2341 taatgtgaaa aataattcct atggacactc cgtttgaagt atcaccttct cataactaaa
     2401 agcagaaaag ctaacaaaag cttctcagct gaggacactc aaggcataca tgatgacagt
     2461 cttttttttt tttgtatgtt aggactttaa cactttatct atggctactg ttattagaac
     2521 aatgtaaatg tatttgctga aagagagcac aaaaatggga gaaaatgcaa acatgagcag
     2581 aaaatatttt cccactggtg tgtagcctgc tacaaggagt tgttgggtta aatgttcatg
     2641 gtcaactcca aggaatactg agatgaaatg tggtaaatca actccacaga accaccaaaa
     2701 agaaaatgag ggtaattcag cttattctga gacagacatt cctggcaatg taccatacaa
     2761 aaaataagcc aactctgaca tttggattct accatagact ctgtcatttt gtagccattt
     2821 cagctgtctt ttgattaatg ttttcgtggc acacatattt ccatcctttt atgtttaatc
     2881 tgtttaaaac aagttcctag tagacaccat ctggttgagt cagttttttt tatggtgtat
     2941 tttgaaccca ttctgatagt ctcttttaac tggaagattt caattactta cgttaatgta
     3001 attattaata tgttaggatt tatcctcagt cagccagttt gttatgtctt ttctattcta
     3061 ctgttatcac atttgtacca cttaaagtgg aatctaggca ctttatcacc atttagatcc
     3121 tattaccttt tctcatctag gatatagtta tcttctacat aatctttctg tatcttaaaa
     3181 cccatcaata aattattata tattttctac ttttaatcac tcagaagatt taaaaaactc
     3241 atgagaagag taatctgtta tgtttttcca gatatttacc atttctgttg ctcttccttc
     3301 attattttcc aaatttcgtt ctgcaaattt ccacttcctc tgatagacgt tttttagttc
     3361 ttttagagtg gttctgatag gtacagattc tcttattttt tgcttcctct gaggacatct
     3421 ttttctcacc ttcattctca gtgatgtttt ttgcttgtag tatttttagt tgacattgtt
     3481 ttctgttcag cagtttcctt ttagcttccg tatttcctga tgagaaatct gcagtcattc
     3541 aaattgttgt ttccctgtat gtagtgtgtc atttttctgt cagatttcaa ggtatttatc
     3601 tttagttttt agccatttca ttatgttggg gatgagtttc cttgttttat tccctttgga
     3661 atttgctcca attcataaat ttgcagtttt atgtctttta ccaaacttag aggttttcag
     3721 cctaatttct aaaaatactt tttattagcc tgattttcat ctttatagga aatagtttaa
     3781 gtgatgacaa gttccaatag cttatatgcc cagaaggcct tcaaaataag aattttgaaa
     3841 gaatacagaa aacaaacttt tatatccttc tcatgtcttc tactgtaaaa ttcatatgct
     3901 ttgctactct aaacctagtt tgaaatcaac agtcttgaga atagatgaaa attttgatga
     3961 atagtggaat tcttttaaat ggaaacctct tacatgtgat tttccttgcc atctagaaat
     4021 aaaccatagt atttatgttg aatcaatcaa tattatattt tgtttttttc ctcctcttct
     4081 gagactctta ttgtggaaat gttagacttt tatgttttcc taaatgtccc tgatattcta
     4141 cttatttaga acatcttttc attttttcca ttattctgat tgggtaattt taatttgtct
     4201 attttcaaat ttgctggagt gttcacctgt tgttatctgt gtcgtcccac tgagtgcatt
     4261 caccaccttt taaattttgg tcactgtatg tatcagttct aaaatttcca ttttgttctc
     4321 tatattttaa atttcttgcc ttatattcta ttttcctgca aatgtgtcag catttgcttg
     4381 tttgagcttt ttttttttca agacagggtc tcaactctgt tacccaggct ggagtgcagt
     4441 ggtgcgatct cagctcactg caacctctgc ctcctggttc aagcgattat tgtgcctcag
     4501 cctccagagt agctgggatt acaggcatgc accaccacag cccagctaat tttttgtatt
     4561 tttagtagag acagagtttt gctatgttgg ccaggctggt tttgaactcc tggcctcaag
     4621 tgatccaccc acctcagcct cccaaagtgc tgggattaca ggccactaca cctggcacat
     4681 ttgagtattt tttttttttt tttttttttt ttgagatgga gtctcgctct gtcatctagg
     4741 ctggagtgca gtggtgtgat ctcagctcac tgcagcctct gtctcccggg ctcaagcgat
     4801 tctcttgcct cagcctcctg agtagctagg actacaggtg catgccaaca cgcccggcta
     4861 attttttttt aaaaatattt ttagtagaga cagggtttca ccattttggc caggatggtc
     4921 ttgatctcct gacctcatga tccacccgcc tcggccttcc aaagtgctgg gattacaggc
     4981 atgagccacc gtgcctggcc tcatttgagt atttttataa tgtctctttt aaagtctttg
     5041 tcagataatt tcactgtaca tgttattcag tgtttggtgt ccactgagtt gtcatttgcc
     5101 agacaagtgg agatttttgc agctcatcct tgtattctca gtagttccga tatgtaccct
     5161 cgaaatgtga atgttatctt atgagactct gttttatttg tatccaacag aagatgttta
     5221 ttatttattt ggctttctgt gaactgaggt cttaatatca gctcatttta aaagtctttg
     5281 cagtggtatt cggatctatc ctgtgtgtgc ctatgagatt gggtgcagtg tatcctgtta
     5341 gctccattct cagggcgttt gaatgtgaat taggaccagc gcaatgaatg ctcaagttgg
     5401 ggttgggcgt tagaattcat aaaagtcttt atatgctcag attctgcagc tccatcctct
     5461 tcatgattgt gatcgtaagg acagtggaat tttgcagttg tctatacctt atcttgaagt
     5521 agaactaaat tgttacattt gaagaatgta cttcataaat ttacaaaaat aaaaaaatag
     5581 gttaatgtta ttaaaacaaa aaggcacaca
//
