LOCUS       NM_001143962            2387 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens calpain 8 (CAPN8), mRNA.
ACCESSION   NM_001143962 XM_001128157 XM_001715279 XM_001715299 XM_927359
            XM_938885
VERSION     NM_001143962.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   D'Adamo,C.R., Dawson,V.J., Ryan,K.A., Yerges-Armstrong,L.M.,
            Semba,R.D., Steinle,N.I., Mitchell,B.D., Shuldiner,A.R. and
            McArdle,P.F.
  TITLE     The CAPN2/CAPN8 Locus on Chromosome 1q Is Associated with Variation
            in Serum Alpha-Carotene Concentrations
  JOURNAL   J Nutrigenet Nutrigenomics 9 (5-6), 254-264 (2016)
   PUBMED   28002826
  REMARK    GeneRIF: 3 novel loci associated with serum alpha-carotene
            concentrations among a population that consumed a controlled diet.
            While replication is necessary, the CAPN2/CAPN8 locus provides
            compelling evidence for an association with serum alpha-carotene
            concentrations and may suggest a relationship with the development
            and progression of cancers.
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Hata,S., Doi,N., Kitamura,F. and Sorimachi,H.
  TITLE     Stomach-specific calpain, nCL-2/calpain 8, is active without
            calpain regulatory subunit and oligomerizes through C2-like domains
  JOURNAL   J Biol Chem 282 (38), 27847-27856 (2007)
   PUBMED   17646163
  REMARK    GeneRIF: nCL-2/calpain 8 is active without calpain regulatory
            subunit and oligomerizes through C2-like domains
REFERENCE   3  (bases 1 to 2387)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   4  (bases 1 to 2387)
  AUTHORS   Hata,S., Nishi,K., Kawamoto,T., Lee,H.J., Kawahara,H., Maeda,T.,
            Shintani,Y., Sorimachi,H. and Suzuki,K.
  TITLE     Both the conserved and the unique gene structure of
            stomach-specific calpains reveal processes of calpain gene
            evolution
  JOURNAL   J Mol Evol 53 (3), 191-203 (2001)
   PUBMED   11523006
REFERENCE   5  (bases 1 to 2387)
  AUTHORS   Huang,Y. and Wang,K.K.
  TITLE     The calpain family and human disease
  JOURNAL   Trends Mol Med 7 (8), 355-362 (2001)
   PUBMED   11516996
  REMARK    Review article
REFERENCE   6  (bases 1 to 2387)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
REFERENCE   7  (bases 1 to 2387)
  AUTHORS   Sorimachi,H., Ishiura,S. and Suzuki,K.
  TITLE     A novel tissue-specific calpain species expressed predominantly in
            the stomach comprises two alternative splicing products with and
            without Ca(2+)-binding domain
  JOURNAL   J Biol Chem 268 (26), 19476-19482 (1993)
   PUBMED   7690035
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA874912.1, BC157893.1, BG187068.1, BM843240.1, FO681503.2 and
            BM739137.1.
            
            On Jul 2, 2019 this sequence version replaced NM_001143962.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC157893.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366872.10/ ENSP00000355837.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                DA874912.1         34-63
            31-788              BC157893.1         1-758
            789-789             BG187068.1         651-651
            790-1829            BC157893.1         760-1799
            1830-2028           BM843240.1         8-206
            2029-2029           FO681503.2         10702-10702         c
            2030-2294           BM843240.1         208-472
            2295-2387           BM739137.1         66-158
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q41"
     gene            1..2387
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /note="calpain 8"
                     /db_xref="GeneID:388743"
                     /db_xref="HGNC:HGNC:1485"
                     /db_xref="MIM:618777"
     exon            1..292
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..2167
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /EC_number="3.4.22.53"
                     /note="new calpain 2; stomach-specific M-type calpain"
                     /codon_start=1
                     /product="calpain-8"
                     /protein_id="NP_001137434.1"
                     /db_xref="CCDS:CCDS73038.1"
                     /db_xref="GeneID:388743"
                     /db_xref="HGNC:HGNC:1485"
                     /db_xref="MIM:618777"
                     /translation="MAAQAAGVSRQRAATQGLGSNQNALKYLGQDFKTLRQQCLDSGV
                     LFKDPEFPACPSALGYKDLGPGSPQTQGIIWKRPTELCPSPQFIVGGATRTDICQGGL
                     GDCWLLAAIASLTLNEELLYRVVPRDQDFQENYAGIFHFQFWQYGEWVEVVIDDRLPT
                     KNGQLLFLHSEQGNEFWSALLEKAYAKLNGCYEALAGGSTVEGFEDFTGGISEFYDLK
                     KPPANLYQIIRKALCAGSLLGCSIDVSSAAEAEAITSQKLVKSHAYSVTGVEEVNFQG
                     HPEKLIRLRNPWGEVEWSGAWSDDAPEWNHIDPRRKEELDKKVEDGEFWMSLSDFVRQ
                     FSRLEICNLSPDSLSSEEVHKWNLVLFNGHWTRGSTAGGCQNYPATYWTNPQFKIRLD
                     EVDEDQEESIGEPCCTVLLGLMQKNRRWRKRIGQGMLSIGYAVYQVPKELESHTDAHL
                     GRDFFLAYQPSARTSTYVNLREVSGRARLPPGEYLVVPSTFEPFKDGEFCLRVFSEKK
                     AQALEIGDVVAGNPYEPHPSEVDQEDDQFRRLFEKLAGKDSEITANALKILLNEAFSK
                     RTDIKFDGFNINTCREMISLLDSNGTGTLGAVEFKTLWLKIQKYLEIYWETDYNHSGT
                     IDAHEMRTALRKAGFTLNSQVQQTIALRYACSKLGINFDSFVACMIRLETLFKLFSLL
                     DEDKDGMVQLSLAEWLCCVLV"
     exon            293..362
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            363..481
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            482..615
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            616..784
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            785..868
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            869..954
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1029
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1190
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1366
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1378
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1590
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1627
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1696
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1754
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1819
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1888
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..1967
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..2084
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2143
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2387
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2368..2373
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /note="hexamer: ATTAAA"
     polyA_site      2387
                     /gene="CAPN8"
                     /gene_synonym="nCL-2"
                     /note="major polyA site"
ORIGIN      
        1 gtctgagagt acagtgcagc cctgcccttc tgtccaccct acagagccca cggccatggc
       61 agcccaggca gctggtgtat ctaggcagcg ggcagccact caaggtcttg gctccaacca
      121 aaacgctttg aagtacttgg gccaggattt caagaccctg aggcaacagt gcttggactc
      181 aggggtccta tttaaggacc ctgagttccc agcatgtcca tcagctttgg gctacaagga
      241 tcttggacca ggctctccgc aaactcaagg catcatctgg aagcggccca cggagttgtg
      301 tcccagccct cagtttatcg ttggtggagc cacgcgcaca gacatttgtc agggtggtct
      361 aggtgactgc tggcttctgg ctgccattgc ctccctgacc ctgaatgaag agctgcttta
      421 ccgggtggtc cccagggacc aggacttcca ggagaactat gcgggaatct ttcactttca
      481 gttctggcag tacggagagt gggtggaggt ggtcattgac gacaggctgc ccaccaagaa
      541 tggacagctg ctcttcctac actcggaaca aggcaatgaa ttctggagtg ccctgctgga
      601 gaaagcctat gccaagctta atggttgtta tgaggctctc gctggaggtt ccacagtgga
      661 ggggtttgag gatttcacag gtggcatctc tgagttttat gacctgaaga aaccaccagc
      721 caatctatat cagatcatcc ggaaggccct ctgtgcgggg tctctgctgg gctgctccat
      781 tgatgtctcc agtgcagccg aagccgaagc catcaccagc cagaagctgg ttaagagtca
      841 tgcgtactct gtcactggag tcgaagaggt gaatttccag ggccatccag agaagctgat
      901 cagactcagg aatccatggg gtgaagtgga gtggtcggga gcctggagcg atgatgcacc
      961 agagtggaat cacatagacc cccggcggaa ggaagaactg gacaagaaag ttgaggatgg
     1021 agaattctgg atgtcacttt cagatttcgt gaggcagttc tctcggttgg agatctgcaa
     1081 cctgtccccg gactctctga gtagcgagga ggtgcacaaa tggaacctgg tcctgttcaa
     1141 cggccactgg acccggggct ccacagctgg gggctgccag aactacccag ccacgtactg
     1201 gaccaatccc cagttcaaaa tccgtttgga tgaagtggat gaggaccagg aggagagcat
     1261 cggtgaaccc tgctgtacag tgctgctggg cctgatgcag aaaaatcgca ggtggcggaa
     1321 gcggatagga caaggcatgc ttagcatcgg ctatgccgtc taccaggttc ccaaggagct
     1381 ggagagtcac acggacgcac acttgggccg ggatttcttc ctggcctacc agccctcagc
     1441 ccgcaccagc acctacgtca acctgcggga ggtctctggc cgggcccggc tgccccctgg
     1501 ggagtacctg gtggtgccat ccacatttga acccttcaaa gacggcgagt tctgcttgag
     1561 agtgttctca gagaagaagg cccaggccct agaaattggg gatgtggtag ctggaaaccc
     1621 atatgagcca catcccagtg aggtggatca ggaagatgac cagttcagga ggctgtttga
     1681 gaagttggca gggaaggatt ctgagattac tgccaatgca ctcaagatac ttttgaatga
     1741 ggcgttttcc aagagaacag acataaaatt cgatggattc aacatcaaca cttgcaggga
     1801 aatgatcagt ctgttggata gcaatggaac gggcactttg ggggcggtgg aattcaagac
     1861 gctctggctg aagattcaga agtatctgga gatctattgg gaaactgatt ataaccactc
     1921 gggcaccatc gatgcccacg agatgaggac agccctcagg aaggcaggtt tcaccctcaa
     1981 cagccaggtg cagcagacca ttgccctgcg gtatgcgtgc agcaagcttg gcatcaactt
     2041 tgacagcttc gtggcttgta tgatccgcct ggagaccctc ttcaaactat tcagccttct
     2101 ggacgaagac aaggatggca tggttcagct ctctctggcc gagtggctgt gctgcgtgtt
     2161 ggtctgaccc ggggtttcgg acatcagtga cactccctgc cccactgctt gcttcttgtc
     2221 accccttctc tacaattttg tgaacattta tgctccagtg gcattcactg gttgttcata
     2281 cctttcttgc cctgggtcta tttcagcagc actgagctat gagctatgta agccgacccg
     2341 gtgggcccag tggagggaaa gcaatcaatt aaagttgtga gccagaa
//
