LOCUS       NM_014296               4348 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens calpain 7 (CAPN7), transcript variant 1, mRNA.
ACCESSION   NM_014296
VERSION     NM_014296.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Kang,N., Shan,H., Wang,J., Mei,J., Jiang,Y., Zhou,J., Huang,C.,
            Zhang,H., Zhang,M., Zhen,X., Yan,G. and Sun,H.
  TITLE     Calpain7 negatively regulates human endometrial stromal cell
            decidualization in EMs by promoting FoxO1 nuclear exclusion via
            hydrolyzing AKT1
  JOURNAL   Biol Reprod 106 (6), 1112-1125 (2022)
   PUBMED   35191464
  REMARK    GeneRIF: Calpain7 negatively regulates human endometrial stromal
            cell decidualization in EMs by promoting FoxO1 nuclear exclusion
            via hydrolyzing AKT1.
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 4348)
  AUTHORS   Maemoto,Y., Ono,Y., Kiso,S., Shibata,H., Takahara,T., Sorimachi,H.
            and Maki,M.
  TITLE     Involvement of calpain-7 in epidermal growth factor receptor
            degradation via the endosomal sorting pathway
  JOURNAL   FEBS J 281 (16), 3642-3655 (2014)
   PUBMED   24953135
  REMARK    GeneRIF: The proteolytic activity of CAPN7 is important for the
            acceleration of EGFR degradation via the endosomal sorting pathway.
REFERENCE   4  (bases 1 to 4348)
  AUTHORS   Liu,H., Jiang,Y., Jin,X., Zhu,L., Shen,X., Zhang,Q., Wang,B.,
            Wang,J., Hu,Y., Yan,G. and Sun,H.
  TITLE     CAPN 7 promotes the migration and invasion of human endometrial
            stromal cell by regulating matrix metalloproteinase 2 activity
  JOURNAL   Reprod Biol Endocrinol 11, 64 (2013)
   PUBMED   23855590
  REMARK    GeneRIF: Data indicate that CAPN 7 promotes endometrial stromal
            cell (hESC) migration and invasion by increasing the activity of
            MMP-2 via an increased ratio of MMP-2 to TIMP-2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4348)
  AUTHORS   Maemoto,Y., Kiso,S., Shibata,H. and Maki,M.
  TITLE     Analysis of limited proteolytic activity of calpain-7 using
            non-physiological substrates in mammalian cells
  JOURNAL   FEBS J 280 (11), 2594-2607 (2013)
   PUBMED   23497113
  REMARK    GeneRIF: Mutational analysis of calpain-7 reveals the importance of
            not only the N-terminal microtubule-interacting and trafficking
            domains but also the C-terminal C2 domain-like domains for
            proteolytic activity.
REFERENCE   6  (bases 1 to 4348)
  AUTHORS   Osako,Y., Maemoto,Y., Tanaka,R., Suzuki,H., Shibata,H. and Maki,M.
  TITLE     Autolytic activity of human calpain 7 is enhanced by
            ESCRT-III-related protein IST1 through MIT-MIM interaction
  JOURNAL   FEBS J 277 (21), 4412-4426 (2010)
   PUBMED   20849418
  REMARK    GeneRIF: Results demonstrate that human calpain 7 is
            proteolytically active, and imply that calpain 7 is activated by
            ESCRT-III-related protein IST1.
REFERENCE   7  (bases 1 to 4348)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 4348)
  AUTHORS   Futai,E., Kubo,T., Sorimachi,H., Suzuki,K. and Maeda,T.
  TITLE     Molecular cloning of PalBH, a mammalian homologue of the
            Aspergillus atypical calpain PalB
  JOURNAL   Biochim Biophys Acta 1517 (2), 316-319 (2001)
   PUBMED   11342116
REFERENCE   9  (bases 1 to 4348)
  AUTHORS   Franz,T., Vingron,M., Boehm,T. and Dear,T.N.
  TITLE     Capn7: a highly divergent vertebrate calpain with a novel
            C-terminal domain
  JOURNAL   Mamm Genome 10 (3), 318-321 (1999)
   PUBMED   10051333
REFERENCE   10 (bases 1 to 4348)
  AUTHORS   Sorimachi,H., Saido,T.C. and Suzuki,K.
  TITLE     New era of calpain research. Discovery of tissue-specific calpains
  JOURNAL   FEBS Lett 343 (1), 1-5 (1994)
   PUBMED   8163008
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI464220.1, AB028639.1,
            BX502535.1, BC056202.1 and BM974327.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014296.2.
            
            Summary: Calpains are ubiquitous, well-conserved family of
            calcium-dependent, cysteine proteases. The calpain proteins are
            heterodimers consisting of an invariant small subunit and variable
            large subunits. The large subunit possesses a cysteine protease
            domain, and both subunits possess calcium-binding domains. Calpains
            have been implicated in neurodegenerative processes, as their
            activation can be triggered by calcium influx and oxidative stress.
            The function of the protein encoded by this gene is not known. An
            orthologue has been found in mouse but it seems to diverge from
            other family members. The mouse orthologue is thought to be calcium
            independent with protease activity. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.187940.1,
                                           SRR1660807.12829.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253693.7/ ENSP00000253693.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-352               BI464220.1         96-447
            353-1545            AB028639.1         354-1546
            1546-1902           BX502535.1         225-581
            1903-3471           BC056202.1         1901-3469
            3472-3735           BC056202.1         3472-3735
            3736-4289           BM974327.1         78-631              c
            4290-4348           BM974327.1         18-76               c
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.1"
     gene            1..4348
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /note="calpain 7"
                     /db_xref="GeneID:23473"
                     /db_xref="HGNC:HGNC:1484"
                     /db_xref="MIM:606400"
     exon            1..352
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     CDS             251..2692
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /note="isoform 1 is encoded by transcript variant 1;
                     calpain like protease; homolog of Aspergillus Nidulans
                     PALB; palB homolog"
                     /codon_start=1
                     /product="calpain-7 isoform 1"
                     /protein_id="NP_055111.1"
                     /db_xref="CCDS:CCDS2624.1"
                     /db_xref="GeneID:23473"
                     /db_xref="HGNC:HGNC:1484"
                     /db_xref="MIM:606400"
                     /translation="MDATALERDAVQFARLAVQRDHEGRYSEAVFYYKEAAQALIYAE
                     MAGSSLENIQEKITEYLERVQALHSAVQSKSADPLKSKHQLDLERAHFLVTQAFDEDE
                     KENVEDAIELYTEAVDLCLKTSYETADKVLQNKLKQLARQALDRAEALSEPLTKPVGK
                     ISSTSVKPKPPPVRAHFPLGANPFLERPQSFISPQSCDAQGQRYTAEEIEVLRTTSKI
                     NGIEYVPFMNVDLRERFAYPMPFCDRWGKLPLSPKQKTTFSKWVRPEDLTNNPTMIYT
                     VSSFSIKQTIVSDCSFVASLAISAAYERRFNKKLITGIIYPQNKDGEPEYNPCGKYMV
                     KLHLNGVPRKVIIDDQLPVDHKGELLCSYSNNKSELWVSLIEKAYMKVMGGYDFPGSN
                     SNIDLHALTGWIPERIAMHSDSQTFSKDNSFRMLYQRFHKGDVLITASTGMMTEAEGE
                     KWGLVPTHAYAVLDIREFKGLRFIQLKNPWSHLRWKGRYSENDVKNWTPELQKYLNFD
                     PRTAQKIDNGIFWISWDDLCQYYDVIYLSWNPGLFKESTCIHSTWDAKQGPVKDAYSL
                     ANNPQYKLEVQCPQGGAAVWVLLSRHITDKDDFANNREFITMVVYKTDGKKVYYPADP
                     PPYIDGIRINSPHYLTKIKLTTPGTHTFTLVVSQYEKQNTIHYTVRVYSACSFTFSKI
                     PSPYTLSKRINGKWSGQSAGGCGNFQETHKNNPIYQFHIEKTGPLLIELRGPRQYSVG
                     FEVVTVSTLGDPGPHGFLRKSSGDYRCGFCYLELENIPSGIFNIIPSTFLPKQEGPFF
                     LDFNSIIPIKITQLQ"
     exon            353..461
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            462..619
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            620..687
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            688..888
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            889..975
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1102
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1188
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1282
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1429
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1536
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1657
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1802
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1902
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2038
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2114
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2260
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2323
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            2324..2454
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2547
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..4348
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3713..3718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /note="hexamer: AATAAA"
     polyA_site      3735
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /note="major polyA site"
     regulatory      4331..4336
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
                     /note="hexamer: AATAAA"
     polyA_site      4348
                     /gene="CAPN7"
                     /gene_synonym="CALPAIN7; PALBH"
ORIGIN      
        1 gagggccgct ggggccgcga agtggggcgg ccgggtgggc tacaagccgg gtctgggctg
       61 aggggcgcgg cttcgcggtg gaccccagcc cggcaacggg aaggcgagct ctcctccacc
      121 gtccaaagta aactttgccg ctccttccgc ggcgctcccg agtcctcgcc gccgccgggc
      181 cgccgcagtc cgcgaagagc cgtcctgcgt cagggcctcc ttccctgccc cggcgcgggg
      241 ccactgcgcc atggacgcca cagcactgga gcgggacgct gtgcagttcg cccgtctggc
      301 ggttcagcgc gaccacgaag gccgctactc cgaggcggtg ttttattaca aggaagctgc
      361 acaagcctta atttatgctg agatggcagg atcaagccta gaaaatattc aagaaaaaat
      421 aactgagtat ctggaaagag ttcaagctct acattcagca gttcagtcaa agagtgctga
      481 tcctttgaag tcaaaacatc agttggactt agagcgtgct catttccttg ttacacaagc
      541 ttttgatgaa gatgaaaaag agaatgttga agatgctata gaattgtaca cagaagctgt
      601 ggatctctgt ctgaaaacat cttatgaaac tgctgataaa gtcctgcaaa ataaactgaa
      661 acagttggct cgacaggcac tagacagagc agaagcgctg agtgagcctt tgaccaagcc
      721 agttggcaaa atcagttcaa caagtgttaa gccaaagcca cctccagtga gagcacattt
      781 tccactgggc gctaatccct tccttgaaag acctcagtca tttataagtc ctcagtcatg
      841 tgatgcacaa ggacagagat acacagcaga agaaatagaa gtactcagga caacatcaaa
      901 aataaatggt atagaatatg ttcctttcat gaatgttgac ctgagagaac gttttgccta
      961 tccaatgcct ttctgtgata gatggggcaa gctaccatta tcacctaaac aaaaaactac
     1021 attttccaag tgggtacgac cagaagacct caccaacaat cctacaatga tatatactgt
     1081 gtccagtttt agcataaagc agacaatagt atcggattgc tcctttgtgg catcactggc
     1141 catcagtgca gcttatgaaa gacgttttaa taagaagtta attaccggca taatttaccc
     1201 tcaaaacaag gatggtgaac cagaatacaa tccatgtggg aagtatatgg taaaacttca
     1261 cctcaatggt gtcccaagaa aggtgataat tgatgaccag ttacctgttg atcacaaggg
     1321 agaattgctc tgttcttatt ccaacaacaa aagtgaatta tgggtttctc tcatagaaaa
     1381 agcatacatg aaagtcatgg gaggatatga ttttccagga tccaactcca atattgatct
     1441 tcatgcactg actggctgga taccagaaag aattgctatg cattcagata gccaaacttt
     1501 cagtaaggat aattctttca gaatgcttta tcaaagattt cacaaaggag atgtcctcat
     1561 cactgcgtca actggaatga tgacagaagc tgaaggagag aagtggggtc tggttcccac
     1621 acacgcatat gctgttttgg atattagaga gttcaagggg ctgcgattta tccagttgaa
     1681 aaatccttgg agtcatttac gttggaaagg aagatacagt gaaaatgatg taaaaaactg
     1741 gactccagag ttgcaaaagt atttaaactt tgatccccga acagctcaga aaatagacaa
     1801 cggaatattt tggatttcct gggatgatct ctgccagtat tatgatgtga tttatttgag
     1861 ttggaatcca ggtcttttta aagaatcaac atgtattcac agtacttggg atgctaagca
     1921 aggacctgtg aaagatgcct atagcctggc caacaacccc cagtacaaac tggaggtgca
     1981 gtgtccacag gggggtgctg cagtttgggt tttgcttagt agacacataa cagacaagga
     2041 tgattttgcg aataatcgag aatttatcac aatggttgta tacaagactg atgggaaaaa
     2101 agtttattac ccagctgacc cacctccata cattgatgga attcgaatta acagccctca
     2161 ttatttgact aagataaagc tgaccacacc tggcacccat acctttacat tagtggtttc
     2221 tcaatatgaa aaacagaaca caatccatta cacggttcgg gtatattcag catgcagctt
     2281 tactttttca aagattcctt caccatacac cttatcaaaa cggattaatg gaaagtggag
     2341 tggtcagagt gctggaggat gtggaaattt ccaagagact cacaaaaata accccatcta
     2401 ccaattccat atagaaaaga ctgggccgtt actgattgag ctacgaggac caaggcaata
     2461 tagcgttgga tttgaggttg taacagtttc tactctagga gatcctggtc cccatggctt
     2521 tctgaggaaa tctagtggtg actataggtg tgggttttgc tacctggaat tagaaaatat
     2581 accttctggg atcttcaata tcattcctag tacctttttg cctaaacaag aaggaccttt
     2641 tttcttggac tttaatagta ttatccccat caagatcaca caacttcagt gatggagaaa
     2701 tctcaagtta ctggctttta tacttaccaa acatcagttc ttcaaataag gacgcaaatc
     2761 ttcaggacag taagcagaac aatcagaatg gaattaaatc tctaaaaacg tgttacagtg
     2821 gaatctggtg cttgtcaggg tgtttggtaa gaactgtata tagtcagaat tacctaaatc
     2881 acctagaggt accgtttaca tggttttgtg tatatagagt tggcttgcat tttaggggcc
     2941 attttgtata aaaagtgcat atgattaaaa ttagactcag tcatcactgt gagatgcctt
     3001 tgctaagagg ataaaggaac tgagaccaga tgagaaaaag aaaggatata gattccttga
     3061 gtggaatagt gggctagatt aatataccga aatatttcca ttgtttccct tttttgcaga
     3121 gcatgtggaa gttaaacctg cttgattcta ctatacatct tgggcaacta gttaccaaat
     3181 gaattgtgcc accataactg attttaattt tgcattattt atgattttaa aatatttgtt
     3241 gcccaggtgt tatgaaagaa taaagctttt aagtatagac taccttagca tgaagatgct
     3301 catgcctaag aatgaaaatt gttgaggtta tctcccattc aatcatgtag caagaactta
     3361 aagaaattca ctactgcagt ttttattttt aaaaaacagt aattgagata ttgaagacat
     3421 tacaatttag tttgtgtggt ctttttttaa attgctgtat cgttcagtct cttgtggcaa
     3481 tagcactttg aagaaaatag agaatttaat atatggtgat tgggatatgt agcattcaaa
     3541 aaaagtgaat tgccaagata ctggtgtcat gtaaattccc actttacata aaaacccatc
     3601 aggacagaat gatgctcaat attttaaaat tctaaaaata gggtgggatt tttcattgtc
     3661 tctactttat aattatcaaa acttattttg tattgctact accttaaatt gaaataaaat
     3721 gtttatactt acggatattg catagtttaa gttagattta ttgaaagatt tcatctgtcg
     3781 tgtttcatgt aaatgagaac agattatttg catgaaaata tatacttcaa caaaaatctg
     3841 ttctttaaca gagtagtggt agattattac actaatgaga tttcactttg gtaaatactt
     3901 catgctttca gttttagcct attaatttta ggtggacaaa tttaacaagt tttctgttac
     3961 tttttaaaaa gaaaaaatcc agaacataag aactatatta tgaacacatg atttgaacct
     4021 gttgtggtaa agatcttgta caggatgcaa actaaaaacc taatccctgc catcaaattt
     4081 attagaagag acctatatat gaacaactta aaggcactga tttctataat agagctctaa
     4141 aaacatgcca ccagtgtatg aataagggaa agattaattt tggctggacc aatataaaaa
     4201 attgtatttg aagaattgat actttaactt ggaccttgaa ggtaaagctt caaaagacag
     4261 gttactgacc attgagtgtt tactatgtac ccaatgtgta tatttttctt tttaatcttc
     4321 ccaatagctg aataaagtat agatacta
//
