LOCUS       NM_015447               8018 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens calmodulin regulated spectrin associated protein 1
            (CAMSAP1), transcript variant 1, mRNA.
ACCESSION   NM_015447 XM_351857
VERSION     NM_015447.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Khalaf-Nazzal,R., Fasham,J., Inskeep,K.A., Blizzard,L.E.,
            Leslie,J.S., Wakeling,M.N., Ubeyratna,N., Mitani,T., Griffith,J.L.,
            Baker,W., Al-Hijawi,F., Keough,K.C., Gezdirici,A., Pena,L.,
            Spaeth,C.G., Turnpenny,P.D., Walsh,J.R., Ray,R., Neilson,A.,
            Kouranova,E., Cui,X., Curiel,D.T., Pehlivan,D., Akdemir,Z.C.,
            Posey,J.E., Lupski,J.R., Dobyns,W.B., Stottmann,R.W., Crosby,A.H.
            and Baple,E.L.
  TITLE     Bi-allelic CAMSAP1 variants cause a clinically recognizable
            neuronal migration disorder
  JOURNAL   Am J Hum Genet 109 (11), 2068-2079 (2022)
   PUBMED   36283405
  REMARK    GeneRIF: Bi-allelic CAMSAP1 variants cause a clinically
            recognizable neuronal migration disorder.
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 8018)
  AUTHORS   Atherton,J., Luo,Y., Xiang,S., Yang,C., Rai,A., Jiang,K.,
            Stangier,M., Vemu,A., Cook,A.D., Wang,S., Roll-Mecak,A.,
            Steinmetz,M.O., Akhmanova,A., Baldus,M. and Moores,C.A.
  TITLE     Structural determinants of microtubule minus end preference in
            CAMSAP CKK domains
  JOURNAL   Nat Commun 10 (1), 5236 (2019)
   PUBMED   31748546
  REMARK    GeneRIF: The CAMSAP1 CKK domain remodels its microtubule
            interaction site and changes the underlying polymer structure
            because the tubulin lattice conformation is not optimal for its
            binding. Thus, in contrast to many microtubule associated proteins,
            the human CKK domain can differentiate subtly specific tubulin
            conformations to enable microtubule minus-end recognition.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8018)
  AUTHORS   Strassburg,S., Nabar,N., Lampert,F., Goerke,S.M., Pfeifer,D.,
            Finkenzeller,G., Stark,G.B. and Simunovic,F.
  TITLE     Calmodulin Regulated Spectrin Associated Protein 1 mRNA is Directly
            Regulated by miR-126 in Primary Human Osteoblasts
  JOURNAL   J Cell Biochem 118 (7), 1756-1763 (2017)
   PUBMED   27958650
  REMARK    GeneRIF: Calmodulin Regulated Spectrin Associated Protein 1 mRNA is
            Directly Regulated by miR-126 in Primary Human Osteoblasts
REFERENCE   5  (bases 1 to 8018)
  AUTHORS   Hendershott,M.C. and Vale,R.D.
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and
            Patronin
  JOURNAL   Proc Natl Acad Sci U S A 111 (16), 5860-5865 (2014)
   PUBMED   24706919
  REMARK    GeneRIF: These results show that members of the CAMSAP/Patronin
            family all localize to and protect minus-ends but have evolved
            distinct effects on microtubule dynamics.
REFERENCE   6  (bases 1 to 8018)
  AUTHORS   Jiang,K., Hua,S., Mohan,R., Grigoriev,I., Yau,K.W., Liu,Q.,
            Katrukha,E.A., Altelaar,A.F., Heck,A.J., Hoogenraad,C.C. and
            Akhmanova,A.
  TITLE     Microtubule minus-end stabilization by polymerization-driven CAMSAP
            deposition
  JOURNAL   Dev Cell 28 (3), 295-309 (2014)
   PUBMED   24486153
REFERENCE   7  (bases 1 to 8018)
  AUTHORS   King,M.D., Phillips,G.W., Bignone,P.A., Hayes,N.V., Pinder,J.C. and
            Baines,A.J.
  TITLE     A conserved sequence in calmodulin regulated spectrin-associated
            protein 1 links its interaction with spectrin and calmodulin to
            neurite outgrowth
  JOURNAL   J Neurochem 128 (3), 391-402 (2014)
   PUBMED   24117850
REFERENCE   8  (bases 1 to 8018)
  AUTHORS   Bai,S.W., Herrera-Abreu,M.T., Rohn,J.L., Racine,V., Tajadura,V.,
            Suryavanshi,N., Bechtel,S., Wiemann,S., Baum,B. and Ridley,A.J.
  TITLE     Identification and characterization of a set of conserved and new
            regulators of cytoskeletal organization, cell morphology and
            migration
  JOURNAL   BMC Biol 9, 54 (2011)
   PUBMED   21834987
  REMARK    Erratum:[BMC Biol. 2024 Oct 10;22(1):228. doi:
            10.1186/s12915-024-02036-3.. PMID: 39390519]
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8018)
  AUTHORS   Baines,A.J., Bignone,P.A., King,M.D., Maggs,A.M., Bennett,P.M.,
            Pinder,J.C. and Phillips,G.W.
  TITLE     The CKK domain (DUF1781) binds microtubules and defines the
            CAMSAP/ssp4 family of animal proteins
  JOURNAL   Mol Biol Evol 26 (9), 2005-2014 (2009)
   PUBMED   19508979
  REMARK    GeneRIF: The CKK domain binds microtubules and represents a domain
            that evolved with the metazoa.
REFERENCE   10 (bases 1 to 8018)
  AUTHORS   Yamamoto,M., Yoshimura,K., Kitada,M., Nakahara,J., Seiwa,C.,
            Ueki,T., Shimoda,Y., Ishige,A., Watanabe,K. and Asou,H.
  TITLE     A new monoclonal antibody, A3B10, specific for astrocyte-lineage
            cells recognizes calmodulin-regulated spectrin-associated protein 1
            (Camsap1)
  JOURNAL   J Neurosci Res 87 (2), 503-513 (2009)
   PUBMED   18756519
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL355574.27, AW448941.1, BU949503.1, BC130580.1 and AL834528.1.
            
            On Nov 23, 2018 this sequence version replaced NM_015447.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ891365.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000389532.9/ ENSP00000374183.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-387               AL355574.27        3984-4370           c
            388-401             AL355574.27        3970-3983           c
            402-836             AW448941.1         1-435
            837-1424            BU949503.1         12-599
            1425-6015           BC130580.1         697-5287
            6016-8018           AL834528.1         1981-3983
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.3"
     gene            1..8018
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /note="calmodulin regulated spectrin associated protein 1"
                     /db_xref="GeneID:157922"
                     /db_xref="HGNC:HGNC:19946"
                     /db_xref="MIM:613774"
     exon            1..547
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     CDS             388..5196
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="calmodulin-regulated spectrin-associated protein
                     1 isoform 1"
                     /protein_id="NP_056262.3"
                     /db_xref="CCDS:CCDS35176.2"
                     /db_xref="GeneID:157922"
                     /db_xref="HGNC:HGNC:19946"
                     /db_xref="MIM:613774"
                     /translation="MVDASGRAAAEGWRKMEAPPDGAADLVPLDRYDAARAKIAANLQ
                     WICAKAYGRDNIPEDLRDPFYVDQYEQEHIKPPVIKLLLSSELYCRVCSLILKGDQVA
                     ALQGHQSVIQALSRKGIYVMESDDTPVTESDLSRAPIKMSAHMAMVDALMMAYTVEMI
                     SIEKVVASVKRFSTFSASKELPYDLEDAMVFWINKVNLKMREITEKEVKLKQQLLESP
                     AHQKVRYRREHLSARQSPYFPLLEDLMRDGSDGAALLAVIHYYCPEQMKLDDICLKEV
                     TSMADSLYNIRLLREFSNEYLNKCFYLTLEDMLYAPLVLKPNVMVFIAELFWWFENVK
                     PDFVQPRDVQELKDAKTVLHQKSSRPPVPISNATKRSFLGSPAAGTLAELQPPVQLPA
                     EGCHRHYLHPEEPEYLGKGTAAFSPSHPLLPLRQKQQKSIQGEDIPDQRHRSNSLTRV
                     DGQPRGAAIAWPEKKTRPASQPTPFALHHAASCEVDPSSGDSISLARSISKDSLASNI
                     VNLTPQNQPHPTATKSHGKSLLSNVSIEDEEEELVAIVRADVVPQQADPEFPRASPRA
                     LGLTANARSPQGQLDTSESKPDSFFLEPLMPAVLKPAKEKQVITKEDERGEGRPRSIV
                     SRRPSEGPQPLVRRKMTGSRDLNRTFTPIPCSEFPMGIDPTETGPLSVETAGEVCGGP
                     LALGGFDPFPQGPSTDGFFLHVGRADEDTEGRLYVSCSKSPNSHDSEPWTLLRQDSDS
                     DVVDIEEAEHDFMGEAHPVVFSRYIGEEESAKLQEDMKVKEHEDKDDASGRSSPCLST
                     ASQMSSVSMASGSVKMTSFAERKLQRLNSCETKSSTSSSQKTTPDASESCPAPLTTWR
                     QKREQSPSQHGKDPASLLASELVQLHMQLEEKRRAIEAQKKKMEALSARQRLKLGKAA
                     FLHVVKKGKAEAAPPLRPEHFAKEYSQHNGEDCGDAVSKTEDFLVKEEQREELLHEPQ
                     DVDKESLAFAQQHKAKDPVALHELERNKVISAALLEDTVGEVVDVNECDLSIEKLNET
                     ISTLQQAILKISQQQEQLLMKSPTVPVPGSKNNSQDHKVKAPVHFVEPLSPTGVAGHR
                     KAPRLGQGRNSRSGRPAELKVPKDRPQGSSRSKTPTPSVETLPHLRPFPASSHPRTPT
                     DPGLDSALEPSGDPHGKCLFDSYRLHDESNQRTLTLSSSKDANILSEQMSLKEVLDAS
                     VKEVGSSSSDVSGKESVPVEEPLRSRASLIEVDLSDLKAPDEDGELVSLDGSADLVSE
                     GDQKPGVGFFFKDEQKAEDELAKKRAAFLLKQQRKAEEARVRKQQLEAEVELKRDEAR
                     RKAEEDRVRKEEEKARRELIKQEYLRRKQQQILEEQGLGKPKSKPKKPRPKSVHREES
                     CSDSGTKCSSTPDNLSRTQSGSSLSLASAATTEPESVHSGGTPSQRVESMEALPILSR
                     NPSRSTDRDWETASAASSLASVAEYTGPKLFKEPSSKSNKPIIHNAISHCCLAGKVNE
                     PHKNSILEELEKCDANHYIILFRDAGCQFRALYCYYPDTEEIYKLTGTGPKNITKKMI
                     DKLYKYSSDRKQFNLIPAKTMSVSVDALTIHNHLWQPKRPAVPKKAQTRK"
     exon            548..810
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            811..972
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1053
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1195
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1335
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1432
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1610
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1702
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1787
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            1788..4209
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            4210..4346
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            4347..4555
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            4556..4658
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            4659..4774
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            4775..4893
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     exon            4894..8018
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6055..6060
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /note="hexamer: AATAAA"
     polyA_site      6082
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /note="major polyA site"
     regulatory      6898..6903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /note="hexamer: AATAAA"
     polyA_site      6931
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /note="major polyA site"
     regulatory      7994..7999
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /note="hexamer: AATAAA"
     polyA_site      8018
                     /gene="CAMSAP1"
                     /gene_synonym="CDCBM12"
                     /note="major polyA site"
ORIGIN      
        1 gagcgcgctc ccgctccttg gccaccgcct cagccgctgt cgccgccgcc gccgctcacc
       61 cgggaccgcg cacgcgctgc caccgccccc ggcccgctcc cgcccccgcg cccgcccccg
      121 cccccggccc gcgccctcct gccgccgcct ctttgggccg cgggctcggc acctcctccg
      181 ctcgggctca gcgcccgcag catgcagcag ccgcgggggc ggcgacggcg gcggctgtga
      241 ggtgaggcgg gggccgcgct cgggcgcccc tcccggctgg ccggctgcgg ggccgagtgg
      301 tggctcgggg cgcaccgggc ccggcgcggc gaggggcggc ggcgcctttg ttgcggccgg
      361 gccgccgcct cagccctttg tctgcagatg gtggacgcga gcggccgcgc cgccgccgag
      421 ggctggagga agatggaggc cccgccggac ggcgccgccg acctcgtgcc cctggaccgc
      481 tacgacgcgg cgcgcgccaa gatcgccgcc aacctgcagt ggatctgcgc caaggcctac
      541 ggccgagata acatccctga ggacctcaga gaccctttct acgttgacca gtatgagcag
      601 gagcacatta agccgcctgt tatcaagctt ctcctgtcca gcgagctgta ctgccgtgtc
      661 tgcagcctca tcctgaaagg ggaccaggtg gccgccttac agggacacca gtctgtcatc
      721 caggccctgt cccggaaagg gatctatgtg atggagagtg atgacacccc cgtgacagag
      781 tccgacctca gtcgcgcacc cataaaaatg agtgcccaca tggcaatggt ggatgccctg
      841 atgatggcct acactgtgga gatgatcagc atcgagaagg tggtggccag tgtcaagcgc
      901 ttctcaacgt tcagtgcctc gaaagaactt ccgtacgacc tcgaggatgc catggtgttc
      961 tggatcaaca aggtaaatct taaaatgaga gagataacag agaaagaagt taaattaaaa
     1021 caacagttat tggaaagtcc agctcatcaa aaggtccgct atcgacgaga gcacctttct
     1081 gctaggcagt caccctactt cccgttgttg gaggatttga tgagagacgg cagtgatggt
     1141 gctgctctct tagctgtgat tcactattat tgcccagagc agatgaaact ggatgatata
     1201 tgcttaaagg aggtaacgtc gatggccgac agtctgtata atattcggct tctgagagaa
     1261 ttctccaatg aatatcttaa taaatgtttt tatctcacct tggaagatat gctgtatgcg
     1321 ccattagtgt tgaagccgaa tgttatggtt tttattgcgg agcttttttg gtggttcgag
     1381 aatgtcaagc cagattttgt tcagcccagg gatgttcagg agctgaaaga cgcgaaaaca
     1441 gtgttacacc agaagagcag ccggcctcct gtaccgatct ccaacgcgac caaacgcagt
     1501 ttcctaggca gccctgctgc agggaccctg gctgagctgc agccccccgt gcagctgccg
     1561 gcggaaggct gtcacaggca ctacctgcac ccggaagaac ctgaatacct cgggaaagga
     1621 actgctgcct tcagcccatc ccatccttta ttgccactga gacagaaaca gcagaaatcc
     1681 atacagggag aggacatccc tgatcagcga catcgatcga attctttgac ccgagttgat
     1741 ggtcagcctc gaggagcagc aatagcctgg ccagaaaaaa aaaccaggcc tgcgtcccag
     1801 ccaacacctt ttgctctaca tcacgctgcg agttgtgaag tggatcccag ctctggcgac
     1861 agcatcagct tggcccgctc catcagcaaa gacagtttgg catccaacat tgttaatctg
     1921 accccacaga accagccaca ccccacggcc acgaagagcc acgggaagag cctcctgagc
     1981 aatgtcagta ttgaggatga ggaagaggag cttgtggcta ttgttagagc agacgtggtt
     2041 ccccagcagg ctgacccgga gttccccagg gcctcacccc gggccttagg ccttacagca
     2101 aatgcccggt ctccccaagg acagctggac acctcggaaa gtaagcctga cagttttttc
     2161 ttggagcctt tgatgccagc agttcttaag ccagcgaaag agaagcaggt gatcaccaag
     2221 gaggatgaac ggggggaagg gagaccgagg agcatcgtgt ctaggaggcc cagcgagggc
     2281 ccccagcctt tggtacgaag gaaaatgact ggcagtcgcg acttgaatag gacttttacc
     2341 ccgattccat gctcagaatt ccccatgggc atcgacccca ccgagacagg accactgtca
     2401 gtggaaaccg caggagaggt ctgtggtggg cctctggccc ttggcggatt cgatccgttc
     2461 ccccagggac catccacgga tggcttcttc cttcatgtag gcagggccga tgaagacacc
     2521 gagggaaggt tatatgttag ttgttcaaaa agccccaact cccacgattc agagccgtgg
     2581 actctcctca ggcaggattc tgactcggat gtggtggaca tagaggaagc cgagcacgat
     2641 ttcatgggtg aggcccatcc tgtggttttc agcagataca ttggggagga agagtcggcc
     2701 aaactgcagg aggacatgaa ggtgaaggaa catgaagaca aagatgacgc cagcggccgc
     2761 tcgagcccct gcctgagcac agcctctcag atgagcagtg tctccatggc gagtgggagc
     2821 gtgaagatga ccagctttgc ggagaggaag ctccagagac tcaacagctg tgagaccaag
     2881 tccagcacca gcagctccca gaagaccacg ccagatgcgt ctgagagctg cccagcccct
     2941 ctgacgacgt ggaggcagaa gagggagcag agtccgagcc agcatggcaa ggatcccgcc
     3001 agcctcctgg catctgagct ggtacagctg cacatgcagc tggaggagaa gcgcagggcc
     3061 atcgaggccc agaagaagaa gatggaggcg ctgtcggcaa ggcagcgcct gaagctcggc
     3121 aaggctgcat tcctgcatgt ggtgaagaag ggcaaggccg aggctgcccc acccctcagg
     3181 ccggagcact ttgcaaagga gtactctcag cacaacgggg aggactgtgg ggacgctgtt
     3241 tccaaaaccg aagactttct cgtgaaggag gagcagagag aggagctcct tcacgagcca
     3301 caggatgtgg acaaagagag cctggccttt gctcagcaac ataaagcaaa agaccctgtg
     3361 gctctgcacg agctggagag aaataaggtg atctccgctg ccctcctgga ggacactgtt
     3421 ggggaggttg tcgacgtgaa tgaatgtgac ctttccatcg agaagcttaa cgaaaccatc
     3481 agtacgctgc agcaggccat cctgaaaatc tctcagcagc aagagcagct tctgatgaag
     3541 tcccccacag tcccagtgcc aggctctaag aataactcgc aggaccacaa agtgaaggca
     3601 ccagtccact tcgtggagcc actctctccc acgggcgtgg ctggccaccg caaagccccc
     3661 cggctgggtc aaggccggaa ttcccgttcc ggaaggccgg cggagctgaa ggtcccaaaa
     3721 gacaggccac agggctcctc ccgaagtaaa accccaacgc ccagtgtaga gacgctcccg
     3781 cacttgagac ccttccctgc cagcagccac cctcggacgc ccacggaccc tggcctggac
     3841 agtgccctgg agcccagtgg tgacccacat gggaagtgtc tcttcgacag ttacaggctc
     3901 catgatgaaa gcaatcagcg gacacttact ctgtcctctt ccaaagatgc caacattctt
     3961 tcggagcaga tgagcctcaa agaagttctg gatgcgagtg tgaaggaggt ggggtccagc
     4021 tcctcagatg tctcgggaaa agagagcgtc cccgtggagg agcctctgag gagcagggcc
     4081 agcctcattg aagtggacct ctccgacctg aaggcccccg acgaggatgg ggagctggta
     4141 agcctcgatg gctcggcgga ccttgtcagc gaaggcgacc agaagccggg ggtcggcttc
     4201 ttcttcaagg atgaacagaa agcggaagat gagctcgcca agaagcgggc ggccttcctc
     4261 ctgaagcagc agcgcaaggc cgaggaggcc cgcgtgcgca agcagcagct ggaagcggag
     4321 gtggagctca agcgtgacga agcccggcgc aaagctgagg aagaccgggt gcggaaggag
     4381 gaggagaagg cgcggcgcga gctcatcaag caggagtacc tgcggaggaa gcagcagcag
     4441 atcctagagg agcaggggct cggcaagccc aagtcaaagc cgaagaagcc gcggccgaag
     4501 tcggtgcacc gggaagagtc gtgcagcgac tccggcacca agtgctcctc cacccctgat
     4561 aacttgagcc ggactcagtc aggctccagc ctgtccttgg cctctgcggc gacgacagaa
     4621 cccgagagcg ttcattccgg gggcacaccc tctcagcgag tggaatcgat ggaagccctg
     4681 cccatactga gccgtaaccc aagcaggagc acagaccgag actgggagac cgcgtcggcg
     4741 gcatcttccc tggcctcagt ggccgagtac acaggtccca agctctttaa ggagcccagt
     4801 agtaaatcaa acaagccgat tattcacaat gccatatccc attgctgcct ggctggaaaa
     4861 gtgaacgaac cccacaagaa ttccatattg gaggagctgg agaagtgtga tgccaatcac
     4921 tacatcatac tgtttcgtga tgctggctgc cagttcaggg cgctttactg ctactatcct
     4981 gatactgagg aaatctacaa actcactggc acggggccaa agaacatcac caagaaaatg
     5041 atcgacaaac tgtataaata cagctcagac cgaaaacagt ttaacttgat cccagccaaa
     5101 accatgtctg tcagtgtgga cgcactcaca atccacaacc acctgtggca gcccaagcgg
     5161 cctgcagtgc caaagaaggc ccagactcgt aaatgacccc ggtgcagctg gcgtccaaag
     5221 ggtgacccag actcgtaaat gatgcccttg tggctggcat ccagagggtg ccacggggtc
     5281 cttatttatt gttttcatct atttgggtac gaagtgtgca aagtcacaga ccttttgcaa
     5341 agaggtttct agacagcaga agaaaacccc tctctcagcg ctcatctcac gcagaggatg
     5401 ccaggcaggg tgaggatggg ggagtagatg ccgcttttgc agtggctcgc actgctgtgg
     5461 ccactgctga ccactgaggc tcccagcgga gggtgttcac agccaggtcc cgggccacgg
     5521 agctccctta cttgaagttc tccttcagca tagattagac gtgatccact ttgctttctt
     5581 gcttctcacc tcgccccctt gcacattgcc tgacaccctc atattggcca gctgtaatct
     5641 cgtgactatg ggactgaggc tcaatcttgg cttggggtgt ttgaatgtct atagcaaagg
     5701 aattcataat gggaaatctt tttcatttga gaaattgtat gatatttatt ggttgtgtct
     5761 actgccaaca tttatatcca attctaagat ttctgaaaaa aaattcttat cccttgctgc
     5821 tcagaaaaag tttacggaaa acaccagttc actaaaagca ccgaaatctg caaagcctgt
     5881 cttccgctag aacaacgtct acaaatgtga agatgatgac ttctggcacg cggtcatgag
     5941 ggtggggaat ggcactagga gctgatgttt gcctcattta aagtaggcaa gccgagccaa
     6001 ctgcagccat cattcattaa gttttgggta gtgctctaat ggtttgttca ctttaataaa
     6061 gacagatttt ggtaaagtag aatatttttc attctgagca tgtttgtccc aaaaggtgat
     6121 gcacttaagc tgacagtccc ttcagtggtt ttgttaggtc acggcttgtc ctgtctggat
     6181 tctgcatctg gaagcgctgc atgctctcag aggagcgtgc cgtttcccct gtagatggat
     6241 acttttgttt tgttgcctat ttaaaaacaa ttaagtggtt atttgcagtt atttcagaac
     6301 tccaagtaag tggatggctt tgggttcttt ctgccttggt cccaatggtg ttgatgcttc
     6361 tgtggagcgc ggacattccc aggagcaggt gcgtgccagc catggctgcc agacactgct
     6421 gcaactgacc agctgccgga agattctgga gccactaaca ttagttttgc acatattttc
     6481 ttgggagtgt tacttgaatc tatctcaaac tgcagaaaaa atcaaaagat caataattgg
     6541 gagaaaaaga gacaaattta actgttacat aattttagat agacaaaaaa catgagtgag
     6601 tcgtgtacat aaggagggaa catggtacag aaggctagaa ggaattatag gtcttattcc
     6661 cctttcctca gtcctttgaa gagaagtaca gtctcttgcg attttgtata tatcgtgttc
     6721 gacacagccg ctctctgtcc tgtaaatagg gaactaaagg ctgtatgatt tctcaagtgc
     6781 tgagttacac agtcctgagt gagctttctg ttaccatact ttcacacgtg ggctttattt
     6841 ctcactgtat gtttgatatg atattactgt atttatttta agaaagcact aagatgtaat
     6901 aaagtgatga actaatttgc tttacattga atcgtatgtg tgaggttgct gtggctcatt
     6961 tcgctgacca ggcgacacca actccttgct ttataggagt ttcacattgt tctttaccat
     7021 ggccctatcg aagtcagtgg aattggattc tttttaatga agagctagaa aatatctggc
     7081 atagagctgg aaaattgcat tcccatggga cgtctgaatc aattctggat tttctccatg
     7141 gaatgagtca gtgtgtggaa catcctgaaa cttgtcctta agcgtgtagt tttcactgtt
     7201 cgttgcgagt aagcactaat gtggcatgga cattcctgat gtcccaagtc cccaggccag
     7261 tgttcgccta atgattgaca gaagcgtccc tgcgttcttc atgcttggac acagcaaccc
     7321 ttttaattag tcttgaaaag tttcagacac aggattaatt ttcgtggtgg tgcttttggg
     7381 cctttctggc ttgggtggtg gtaaagtcat gattttgcag ttgataacac tgacttataa
     7441 ctctgtttat caatgtctcc tatattcaaa gcccctgcag tggtatttgt gtctcgttgc
     7501 aaatttcttt cagcggaaag cttgcacaac tttcgttgtg tctcagaatt ctaaccttgt
     7561 tatttaagac aagctgctct acccatttag gatataactt tgtaaagaaa gtgtaaacca
     7621 aatgattcaa tgtatgatga agtttatgtg taaatccttg gtaatgctag aattctggga
     7681 gcccagaagg gttgaaagag aaatgaaact tgcgtgagtc ccattatttt acgcatgtat
     7741 gtgcagatac gttctaccca cacgtgtgcg tgcacatggc tgtgtgcgtg caccaaagat
     7801 ggactgcttt ccatgtgtcc ttttgacttt ctgcacgtgt cactgcggtg cagtctctta
     7861 gcagacttca ggcccaaact gtattcttca ctcaggcaaa attgaaaagt ggaataattc
     7921 taaattactt ctaggttata cttttacctc cctgaaattg tagttgtcac ttggagggca
     7981 aaatatttat tgaaataaaa ttttctgtta aaaattca
//
