LOCUS       NM_001219               5266 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens calumenin (CALU), transcript variant 1, mRNA.
ACCESSION   NM_001219
VERSION     NM_001219.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 5266)
  AUTHORS   Nasri Nasrabadi,P., Nayeri,Z., Gharib,E., Salmanipour,R.,
            Masoomi,F., Mahjoubi,F. and Zomorodipour,A.
  TITLE     Establishment of a CALU, AURKA, and MCM2 gene panel for
            discrimination of metastasis from primary colon and lung cancers
  JOURNAL   PLoS One 15 (5), e0233717 (2020)
   PUBMED   32469983
  REMARK    GeneRIF: Establishment of a CALU, AURKA, and MCM2 gene panel for
            discrimination of metastasis from primary colon and lung cancers.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5266)
  AUTHORS   Narayanasamy,S. and Aradhyam,G.K.
  TITLE     The Differential Response to Ca2+ from Vertebrate and Invertebrate
            Calumenin Is Governed by a Single Amino Acid Residue
  JOURNAL   Biochemistry 57 (5), 722-731 (2018)
   PUBMED   29319298
  REMARK    GeneRIF: Replacing Leu with Gly (L150G) in Calu1 enables the
            protein to adopt a structural fold even in the Ca(2+) free form.
            The fourth (of seven) EF-hand of HsCalu1 nucleates the structural
            fold of the protein depending on the switch residue (Gly or Leu).
REFERENCE   5  (bases 1 to 5266)
  AUTHORS   Philippe,R., Antigny,F., Buscaglia,P., Norez,C., Huguet,F.,
            Castelbou,C., Trouve,P., Becq,F., Frieden,M., Ferec,C. and
            Mignen,O.
  TITLE     Calumenin contributes to ER-Ca2+ homeostasis in bronchial
            epithelial cells expressing WT and F508del mutated CFTR and to
            F508del-CFTR retention
  JOURNAL   Cell Calcium 62, 47-59 (2017)
   PUBMED   28189267
  REMARK    GeneRIF: The Calumenin modulates Sarco/Endoplasmic
            reticulumCa2+ATPase (SERCA) pump activity without drastically
            affecting ER-Ca(2+) concentration.
REFERENCE   6  (bases 1 to 5266)
  AUTHORS   Vorum,H., Hager,H., Christensen,B.M., Nielsen,S. and Honore,B.
  TITLE     Human calumenin localizes to the secretory pathway and is secreted
            to the medium
  JOURNAL   Exp Cell Res 248 (2), 473-481 (1999)
   PUBMED   10222138
REFERENCE   7  (bases 1 to 5266)
  AUTHORS   Hseu,M.J., Yen,C.H. and Tzeng,M.C.
  TITLE     Crocalbin: a new calcium-binding protein that is also a binding
            protein for crotoxin, a neurotoxic phospholipase A2
  JOURNAL   FEBS Lett 445 (2-3), 440-444 (1999)
   PUBMED   10094503
REFERENCE   8  (bases 1 to 5266)
  AUTHORS   Vorum,H., Liu,X., Madsen,P., Rasmussen,H.H. and Honore,B.
  TITLE     Molecular cloning of a cDNA encoding human calumenin, expression in
            Escherichia coli and analysis of its Ca2+-binding activity
  JOURNAL   Biochim Biophys Acta 1386 (1), 121-131 (1998)
   PUBMED   9675259
REFERENCE   9  (bases 1 to 5266)
  AUTHORS   Yabe,D., Taniwaki,M., Nakamura,T., Kanazawa,N., Tashiro,K. and
            Honjo,T.
  TITLE     Human calumenin gene (CALU): cDNA isolation and chromosomal mapping
            to 7q32
  JOURNAL   Genomics 49 (2), 331-333 (1998)
   PUBMED   9598325
REFERENCE   10 (bases 1 to 5266)
  AUTHORS   Yabe,D., Nakamura,T., Kanazawa,N., Tashiro,K. and Honjo,T.
  TITLE     Calumenin, a Ca2+-binding protein retained in the endoplasmic
            reticulum with a novel carboxyl-terminal sequence, HDEF
  JOURNAL   J Biol Chem 272 (29), 18232-18239 (1997)
   PUBMED   9218460
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK074862.1, HM002604.1,
            AC024952.4 and BF438624.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001219.4.
            
            Summary: The product of this gene is a calcium-binding protein
            localized in the endoplasmic reticulum (ER) and it is involved in
            such ER functions as protein folding and sorting. This protein
            belongs to a family of multiple EF-hand proteins (CERC) that
            include reticulocalbin, ERC-55, and Cab45 and the product of this
            gene. Alternatively spliced transcript variants encoding different
            isoforms have been identified. [provided by RefSeq, Oct 2008].
            
            Transcript Variant: This variant (1) encodes the predominant
            isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.228938.1,
                                           SRR1660807.61266.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000249364.9/ ENSP00000249364.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-833               AK074862.1         18-850
            834-3343            HM002604.1         1717-4226
            3344-5131           AC024952.4         55935-57722
            5132-5266           BF438624.1         34-168              c
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q32.1"
     gene            1..5266
                     /gene="CALU"
                     /note="calumenin"
                     /db_xref="GeneID:813"
                     /db_xref="HGNC:HGNC:1458"
                     /db_xref="MIM:603420"
     exon            1..74
                     /gene="CALU"
                     /inference="alignment:Splign:2.1.0"
     exon            75..306
                     /gene="CALU"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1033
                     /gene="CALU"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; crocalbin-like protein; multiple EF-hand
                     protein; IEF SSP 9302"
                     /codon_start=1
                     /product="calumenin isoform a precursor"
                     /protein_id="NP_001210.1"
                     /db_xref="CCDS:CCDS5805.1"
                     /db_xref="GeneID:813"
                     /db_xref="HGNC:HGNC:1458"
                     /db_xref="MIM:603420"
                     /translation="MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQS
                     FDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQK
                     RWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRF
                     KMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMY
                     SHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYE
                     SDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF"
     exon            307..500
                     /gene="CALU"
                     /inference="alignment:Splign:2.1.0"
     exon            501..667
                     /gene="CALU"
                     /inference="alignment:Splign:2.1.0"
     exon            668..728
                     /gene="CALU"
                     /inference="alignment:Splign:2.1.0"
     exon            729..928
                     /gene="CALU"
                     /inference="alignment:Splign:2.1.0"
     exon            929..5266
                     /gene="CALU"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3321..3326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALU"
                     /note="hexamer: AATAAA"
     polyA_site      3340
                     /gene="CALU"
                     /note="major polyA site"
     regulatory      3699..3704
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALU"
                     /note="hexamer: ATTAAA"
     polyA_site      3717
                     /gene="CALU"
     regulatory      5099..5104
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALU"
                     /note="hexamer: ATTAAA"
     polyA_site      5123
                     /gene="CALU"
     regulatory      5248..5253
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALU"
                     /note="hexamer: AATAAA"
     polyA_site      5266
                     /gene="CALU"
ORIGIN      
        1 gcttgcgcgc gtgagctgag ccggtgggtg agcggcggcc acggcatcct gtgctgtggg
       61 ggctacgagg aaagatctaa ttatcatgga cctgcgacag tttcttatgt gcctgtccct
      121 gtgcacagcc tttgccttga gcaaacccac agaaaagaag gaccgtgtac atcatgagcc
      181 tcagctcagt gacaaggttc acaatgatgc tcagagtttt gattatgacc atgatgcctt
      241 cttgggtgct gaagaagcaa agacctttga tcagctgaca ccagaagaga gcaaggaaag
      301 gcttggaaag attgtaagta aaatagatgg cgacaaggac gggtttgtca ctgtggatga
      361 gctcaaagac tggattaaat ttgcacaaaa gcgctggatt tacgaggatg tagagcgaca
      421 gtggaagggg catgacctca atgaggacgg cctcgtttcc tgggaggagt ataaaaatgc
      481 cacctacggc tacgttttag atgatccaga tcctgatgat ggatttaact ataaacagat
      541 gatggttaga gatgagcgga ggtttaaaat ggcagacaag gatggagacc tcattgccac
      601 caaggaggag ttcacagctt tcctgcaccc tgaggagtat gactacatga aagatatagt
      661 agtacaggaa acaatggaag atatagataa gaatgctgat ggtttcattg atctagaaga
      721 gtatattggt gacatgtaca gccatgatgg gaatactgat gagccagaat gggtaaagac
      781 agagcgagag cagtttgttg agtttcggga taagaaccgt gatgggaaga tggacaagga
      841 agagaccaaa gactggatcc ttccctcaga ctatgatcat gcagaggcag aagccaggca
      901 cctggtctat gaatcagacc aaaacaagga tggcaagctt accaaggagg agatcgttga
      961 caagtatgac ttatttgttg gcagccaggc cacagatttt ggggaggcct tagtacggca
     1021 tgatgagttc tgagctacgg aggaaccctc atttcctcaa aagtaattta tttttacagc
     1081 ttctggtttc acatgaaatt gtttgcgcta ctgagactgt tactacaaac tttttaagac
     1141 atgaaaaggc gtaatgaaaa ccatcccgtc cccattcctc ctcctctctg agggactgga
     1201 gggaagccgt gcttctgagg aacaactcta attagtacac ttgtgtttgt agatttacac
     1261 tttgtattat gtattaacat ggcgtgttta tttttgtatt tttctctggt tgggagtatg
     1321 atatgaagga tcaagatcct caactcacac atgtagacaa acattagctc tttactcttt
     1381 ctcaacccct tttatgattt taataattct cacttaacta attttgtaag cctgagatca
     1441 ataagaaatg ttcaggagag aggaaagaaa aaaaatatat gctccacaat ttatatttag
     1501 agagagaaca cttagtcttg cctgtcaaaa agtccaacat ttcataggta gtaggggcca
     1561 catattacat tcagttgcta taggtccagc aactgaacct gccattacct gggcaaggaa
     1621 agatcccttt gctctaggaa agcttggccc aaattgattt tcttcttttt ccccctgtag
     1681 gactgactgt tggctaattt tgtcaagcac agctgtggtg ggaagagtta gggccagtgt
     1741 cttgaaaatc aatcaagtag tgaatgtgat ctctttgcag agctatagat agaaacagct
     1801 ggaaaactaa aggaaaaata caagtgtttt cggggcatac attttttttc tgggtgtgca
     1861 tctgttgaaa tgctcaagac ttaattattt gccttttgaa atcactgtaa atgcccccat
     1921 ccggttcctc ttcttcccag gtgtgccaag gaattaatct tggtttcact acaattaaaa
     1981 ttcactcctt tccaatcatg tcattgaaag tgcctttaac gaaagaaatg gtcactgaat
     2041 gggaattctc ttaagaaacc ctgagattaa aaaaagacta tttggataac ttataggaaa
     2101 gcctagaacc tcccagtaga gtggggattt ttttcttctt ccctttctct tttggacaat
     2161 agttaaatta gcagtattag ttatgagttt ggttgcagtg ttcttatctt gtgggctgat
     2221 ttccaaaaac cacatgctgc tgaatttacc agggatcctc atacctcaca atgcaaacca
     2281 cttactacca ggcctttttc tgtgtccact ggagagcttg agctcacact caaagatcag
     2341 aggacctaca gagagggctc tttggtttga ggaccatggc ttacctttcc tgcctttgac
     2401 ccatcacacc ccatttcctc ctctttccct ctccccgctg ccaaaaaaaa aaaaaaagga
     2461 aacgtttatc atgaatcaac agggtttcag tccttatcaa agagagatgt ggaaagagct
     2521 aaagaaacca ccctttgttc ccaactccac tttacccata ttttatgcaa cacaaacact
     2581 gtccttttgg gtccctttct tacagatgga cctcttgaga agaattatcg tattccacgt
     2641 ttttagccct caggttacca agataaatat atgtatatat aacctttatt attgctatat
     2701 ctttgtggat aatacattca ggtggtgctg ggtgatttat tataatctga acctaggtat
     2761 atcctttggt cttccacagt catgttgagg tgggctccct ggtatggtaa aaagccaggt
     2821 ataatgtaac ttcaccccag cctttgtact aagctcttga tagtggatat actcttttaa
     2881 gtttagcccc aatatagggt aatggaaatt tcctgccctc tgggttcccc atttttacta
     2941 ttaagaagac cagtgataat ttaataatgc caccaactct ggcttagtta agtgagagtg
     3001 tgaactgtgt ggcaagagag cctcacacct cactaggtgc agagagccca ggccttatgt
     3061 taaaatcatg cacttgaaaa gcaaacctta atctgcaaag acagcagcaa gcattatacg
     3121 gtcatcttga atgatccctt tgaaattttt tttttgtttg tttgtttaaa tcaagcctga
     3181 ggctggtgaa cagtagctac acacccatat tgtgtgttct gtgaatgcta gctctcttga
     3241 atttggatat tggttatttt ttatagagtg taaaccaagt tttatattct gcaatgcgaa
     3301 caggtaccta tctgtttcta aataaaactg tttacattca ttatggggta tgtatgacct
     3361 tcattttcca agaaatagaa ctctagctta gaattatgga tgctctaaaa tgtcagaatg
     3421 ggaactctcc tcgaagttct cccaaactca gagacagcac tgccttctcc taaatgatta
     3481 ttcttttctc cctgttttct ggtattttct agcatccttc tcaccacagc cataaccctt
     3541 ttttacttcc attaggccgt ataactggag agacctgctg ctcgttatat aattatctga
     3601 taccaaacac aagcgattct gaatgtacac acacgtttat ctatagagca taaggtaaaa
     3661 caattttgtt taacattttg gtattgttta caaatgttat taaaacatgc aaattcattt
     3721 tgagaacctc gagagaatta aactattaac tttagtaaac acacagttta agacaaatat
     3781 aaaggtttag ccaaatgcaa cggggaggaa gcacctggaa taatttgtgc ttgctcaggg
     3841 gcttgtggac atggctgttc ctgccaaagc tgtagcagtt atccaaaagt tctttcaaac
     3901 tcatatttgg ggccactgag ttttttttgt tttttttttt gttttgtttt gttttttctt
     3961 tatgtctctc ccatttctaa agtgttttct taagtttgtt ctaattcagc atatatcatc
     4021 tcagaatgga tccccagcag gaaaaaaaaa aaaagttact aaaaaggaaa gtatccatac
     4081 actgaaaact taaagcatat tcctttttgg ttgccttgta tgtagaaatc agttctccaa
     4141 gtgctgtgaa tggtgccact taggagtaga aactgatgat tgttgaaatg gcctttgggt
     4201 ggtctgaaat agaccagtgg aaacagtagc tttgtaggca agaaggcaat agtaagaaaa
     4261 tgaaaaattg actccccaaa ctgccatcac tccttttacc atcttttttg tgtttttagt
     4321 agtttggttt ctgacggaga caatagaaac ttacttaaaa gtaaaattta attctagctg
     4381 ttgcaaacag gccaatattg ggtcctcagt tggggccaac ttgggtagac gagacagtag
     4441 aaacttctgt tttctgggag ctgcatgtgt cggattcatc tgtcatggcc ttcccacaca
     4501 ccatcttcat gatgcaagct tggaactgga gagaaaggta caattggaga taaccttggc
     4561 agatgaggaa gtatgggaaa aaagacctta ttctctgtat caccaaagcc ttgcacaatg
     4621 ctttgtaccc agaatgccct taggtggttt tgaatctatc ttccccatcc tgaaaactat
     4681 ctagattcca gtctagaact actcctgggt ctacggtaat cctaaactgg tcaagtctga
     4741 gacctcactg ttggtaaatt cacaaaccat cctgcttcat aaaagcactg tcagcagagg
     4801 cccctccatt actagcaggt gctcccagct ggaaaggact gccaattctc ttctatccag
     4861 ggaggtttca aattagtttt gagtctacct ctgggattga ggaaacaata ccatctgaaa
     4921 gtagggtttt tgccacttag ggtttggtgt ttattagctc ggatttcaga tgggatagga
     4981 aatcccccag taccttcaga tgataagata ttttagagat ttttgcatgt ggtatgattt
     5041 ctgtgcttca cacatgctcg tgcaggctaa aagttggaag ggaaatgatt aagaaattat
     5101 taaatgcaag gactgaaaat tgagaggaat ctgagttttt atttttattt ttttaatttt
     5161 atttaagttc caggatacat gtgcaggatg tgcaggtttg ttacataggt aaacgtgtgc
     5221 catggtggtt tgctgcacga gtttttcaat aaaatgcttt cattca
//
