LOCUS       NM_004343               1901 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens calreticulin (CALR), mRNA.
ACCESSION   NM_004343
VERSION     NM_004343.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Fan,J., Gillespie,K.P., Mesaros,C. and Blair,I.A.
  TITLE     HMGB2-induced calreticulin translocation required for immunogenic
            cell death and ferroptosis of cancer cells are controlled by the
            nuclear exporter XPO1
  JOURNAL   Commun Biol 7 (1), 1234 (2024)
   PUBMED   39354146
  REMARK    GeneRIF: HMGB2-induced calreticulin translocation required for
            immunogenic cell death and ferroptosis of cancer cells are
            controlled by the nuclear exporter XPO1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Wang,Y.N., Cao,D., Liu,J., Ren,Q.N., Weng,N.Q., Zhou,Y.F.,
            Zhang,M.Y., Wang,S.C., Chen,M.S., Mai,S.J. and Wang,H.Y.
  TITLE     CircATF6 inhibits hepatocellular carcinoma progression by
            suppressing calreticulin-mediated Wnt/beta-catenin signaling
            pathway
  JOURNAL   Cell Signal 122, 111298 (2024)
   PUBMED   39004325
  REMARK    GeneRIF: CircATF6 inhibits hepatocellular carcinoma progression by
            suppressing calreticulin-mediated Wnt/beta-catenin signaling
            pathway.
REFERENCE   3  (bases 1 to 1901)
  AUTHORS   Vadeikiene,R., Jakstys,B., Laukaitiene,D., Satkauskas,S.,
            Juozaityte,E. and Ugenskiene,R.
  TITLE     The Role of Mutated Calreticulin in the Pathogenesis of
            BCR-ABL1-Negative Myeloproliferative Neoplasms
  JOURNAL   Int J Mol Sci 25 (18), 9873 (2024)
   PUBMED   39337361
  REMARK    GeneRIF: The Role of Mutated Calreticulin in the Pathogenesis of
            BCR-ABL1-Negative Myeloproliferative Neoplasms.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1901)
  AUTHORS   Haage,T.R., Charakopoulos,E., Bhuria,V., Baldauf,C.K., Korthals,M.,
            Handschuh,J., Muller,P., Li,J., Harit,K., Nishanth,G., Frey,S.,
            Bottcher,M., Fischer,K.D., Dudeck,J., Dudeck,A., Lipka,D.B.,
            Schraven,B., Green,A.R., Muller,A.J., Mougiakakos,D. and Fischer,T.
  TITLE     Neutrophil-specific expression of JAK2-V617F or CALRmut induces
            distinct inflammatory profiles in myeloproliferative neoplasia
  JOURNAL   J Hematol Oncol 17 (1), 43 (2024)
   PUBMED   38853260
  REMARK    GeneRIF: Neutrophil-specific expression of JAK2-V617F or CALRmut
            induces distinct inflammatory profiles in myeloproliferative
            neoplasia.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1901)
  AUTHORS   Liang,L., Wang,J., Guo,T., Huang,L., Wu,Y., Xu,R., Huang,T. and
            Ma,B.
  TITLE     Calreticulin regulates the expression of MMP14 and ADAR1 through
            EIF2AK2 signaling to promote the proliferation and progression of
            malignant melanoma cells
  JOURNAL   Neoplasma 71 (2), 180-192 (2024)
   PUBMED   38766853
  REMARK    GeneRIF: Calreticulin regulates the expression of MMP14 and ADAR1
            through EIF2AK2 signaling to promote the proliferation and
            progression of malignant melanoma cells.
REFERENCE   6  (bases 1 to 1901)
  AUTHORS   Hochstrasser,D.F., Frutiger,S., Paquet,N., Bairoch,A., Ravier,F.,
            Pasquali,C., Sanchez,J.C., Tissot,J.D., Bjellqvist,B., Vargas,R. et
            al.
  TITLE     Human liver protein map: a reference database established by
            microsequencing and gel comparison
  JOURNAL   Electrophoresis 13 (12), 992-1001 (1992)
   PUBMED   1286669
REFERENCE   7  (bases 1 to 1901)
  AUTHORS   Rasmussen,H.H., van Damme,J., Puype,M., Gesser,B., Celis,J.E. and
            Vandekerckhove,J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
REFERENCE   8  (bases 1 to 1901)
  AUTHORS   McCauliffe,D.P., Yang,Y.S., Wilson,J., Sontheimer,R.D. and
            Capra,J.D.
  TITLE     The 5'-flanking region of the human calreticulin gene shares
            homology with the human GRP78, GRP94, and protein disulfide
            isomerase promoters
  JOURNAL   J Biol Chem 267 (4), 2557-2562 (1992)
   PUBMED   1733953
REFERENCE   9  (bases 1 to 1901)
  AUTHORS   Rokeach,L.A., Haselby,J.A., Meilof,J.F., Smeenk,R.J., Unnasch,T.R.,
            Greene,B.M. and Hoch,S.O.
  TITLE     Characterization of the autoantigen calreticulin
  JOURNAL   J Immunol 147 (9), 3031-3039 (1991)
   PUBMED   1919005
REFERENCE   10 (bases 1 to 1901)
  AUTHORS   Rojiani,M.V., Finlay,B.B., Gray,V. and Dedhar,S.
  TITLE     In vitro interaction of a polypeptide homologous to human Ro/SS-A
            antigen (calreticulin) with a highly conserved amino acid sequence
            in the cytoplasmic domain of integrin alpha subunits
  JOURNAL   Biochemistry 30 (41), 9859-9866 (1991)
   PUBMED   1911778
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092069.2.
            
            On Oct 11, 2019 this sequence version replaced NM_004343.3.
            
            Summary: Calreticulin is a highly conserved chaperone protein which
            resides primarily in the endoplasmic reticulum, and is involved in
            a variety of cellular processes, among them, cell adhesion.
            Additionally, it functions in protein folding quality control and
            calcium homeostasis. Calreticulin is also found in the nucleus,
            suggesting that it may have a role in transcription regulation.
            Systemic lupus erythematosus is associated with increased
            autoantibody titers against calreticulin. Recurrent mutations in
            calreticulin have been linked to various neoplasms, including the
            myeloproliferative type.[provided by RefSeq, May 2020].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK223060.1, M84739.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316448.10/ ENSP00000320866.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               AC092069.2         50907-51068         c
            163-264             AC092069.2         50442-50543         c
            265-468             AC092069.2         50046-50249         c
            469-563             AC092069.2         49530-49624         c
            564-773             AC092069.2         49225-49434         c
            774-887             AC092069.2         49023-49136         c
            888-1031            AC092069.2         48790-48933         c
            1032-1124           AC092069.2         46048-46140         c
            1125-1901           AC092069.2         45188-45964         c
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.13"
     gene            1..1901
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /note="calreticulin"
                     /db_xref="GeneID:811"
                     /db_xref="HGNC:HGNC:1455"
                     /db_xref="MIM:109091"
     exon            1..162
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..1325
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /note="Sicca syndrome antigen A (autoantigen Ro;
                     calreticulin); CRP55; ERp60; HACBP; grp60; calregulin;
                     endoplasmic reticulum resident protein 60; epididymis
                     secretory sperm binding protein Li 99n"
                     /codon_start=1
                     /product="calreticulin precursor"
                     /protein_id="NP_004334.1"
                     /db_xref="CCDS:CCDS12288.1"
                     /db_xref="GeneID:811"
                     /db_xref="HGNC:HGNC:1455"
                     /db_xref="MIM:109091"
                     /translation="MLLSVPLLLGLLGLAVAEPAVYFKEQFLDGDGWTSRWIESKHKS
                     DFGKFVLSSGKFYGDEEKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQN
                     IDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKD
                     IRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDASKPED
                     WDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWK
                     PRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITN
                     DEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKD
                     EDEEDEEDKEEDEEEDVPGQAKDEL"
     exon            163..264
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     exon            265..468
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     exon            469..563
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     exon            564..773
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     exon            774..887
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1031
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1124
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1901
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1885..1890
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /note="hexamer: ATTAAA"
     polyA_site      1901
                     /gene="CALR"
                     /gene_synonym="CALR1; cC1qR; CRT; HEL-S-99n; RO; SSA"
                     /note="major polyA site"
ORIGIN      
        1 gtccgtactg cagagccgct gccggagggt cgttttaaag ggcccgcgcg ttgccgcccc
       61 ctcggcccgc catgctgcta tccgtgccgc tgctgctcgg cctcctcggc ctggccgtcg
      121 ccgagcctgc cgtctacttc aaggagcagt ttctggacgg agacgggtgg acttcccgct
      181 ggatcgaatc caaacacaag tcagattttg gcaaattcgt tctcagttcc ggcaagttct
      241 acggtgacga ggagaaagat aaaggtttgc agacaagcca ggatgcacgc ttttatgctc
      301 tgtcggccag tttcgagcct ttcagcaaca aaggccagac gctggtggtg cagttcacgg
      361 tgaaacatga gcagaacatc gactgtgggg gcggctatgt gaagctgttt cctaatagtt
      421 tggaccagac agacatgcac ggagactcag aatacaacat catgtttggt cccgacatct
      481 gtggccctgg caccaagaag gttcatgtca tcttcaacta caagggcaag aacgtgctga
      541 tcaacaagga catccgttgc aaggatgatg agtttacaca cctgtacaca ctgattgtgc
      601 ggccagacaa cacctatgag gtgaagattg acaacagcca ggtggagtcc ggctccttgg
      661 aagacgattg ggacttcctg ccacccaaga agataaagga tcctgatgct tcaaaaccgg
      721 aagactggga tgagcgggcc aagatcgatg atcccacaga ctccaagcct gaggactggg
      781 acaagcccga gcatatccct gaccctgatg ctaagaagcc cgaggactgg gatgaagaga
      841 tggacggaga gtgggaaccc ccagtgattc agaaccctga gtacaagggt gagtggaagc
      901 cccggcagat cgacaaccca gattacaagg gcacttggat ccacccagaa attgacaacc
      961 ccgagtattc tcccgatccc agtatctatg cctatgataa ctttggcgtg ctgggcctgg
     1021 acctctggca ggtcaagtct ggcaccatct ttgacaactt cctcatcacc aacgatgagg
     1081 catacgctga ggagtttggc aacgagacgt ggggcgtaac aaaggcagca gagaaacaaa
     1141 tgaaggacaa acaggacgag gagcagaggc ttaaggagga ggaagaagac aagaaacgca
     1201 aagaggagga ggaggcagag gacaaggagg atgatgagga caaagatgag gatgaggagg
     1261 atgaggagga caaggaggaa gatgaggagg aagatgtccc cggccaggcc aaggacgagc
     1321 tgtagagagg cctgcctcca gggctggact gaggcctgag cgctcctgcc gcagagctgg
     1381 ccgcgccaaa taatgtctct gtgagactcg agaactttca tttttttcca ggctggttcg
     1441 gatttggggt ggattttggt tttgttcccc tcctccactc tcccccaccc cctccccgcc
     1501 cttttttttt ttttttttta aactggtatt ttatctttga ttctccttca gccctcaccc
     1561 ctggttctca tctttcttga tcaacatctt ttcttgcctc tgtccccttc tctcatctct
     1621 tagctcccct ccaacctggg gggcagtggt gtggagaagc cacaggcctg agatttcatc
     1681 tgctctcctt cctggagccc agaggagggc agcagaaggg ggtggtgtct ccaacccccc
     1741 agcactgagg aagaacgggg ctcttctcat ttcacccctc cctttctccc ctgcccccag
     1801 gactgggcca cttctgggtg gggcagtggg tcccagattg gctcacactg agaatgtaag
     1861 aactacaaac aaaatttcta ttaaattaaa ttttgtgtct c
//
