LOCUS       NM_031468               9424 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens calneuron 1 (CALN1), transcript variant 1, mRNA.
ACCESSION   NM_031468
VERSION     NM_031468.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Angelats,E., Requesens,M., Aguinaga,D., Kreutz,M.R., Franco,R. and
            Navarro,G.
  TITLE     Neuronal Calcium and cAMP Cross-Talk Mediated by Cannabinoid CB1
            Receptor and EF-Hand Calcium Sensor Interactions
  JOURNAL   Front Cell Dev Biol 6, 67 (2018)
   PUBMED   30073165
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9424)
  AUTHORS   Gong,L., Bao,Q., Hu,C., Wang,J., Zhou,Q., Wei,L., Tong,L., Zhang,W.
            and Shen,Y.
  TITLE     Exosomal miR-675 from metastatic osteosarcoma promotes cell
            migration and invasion by targeting CALN1
  JOURNAL   Biochem Biophys Res Commun 500 (2), 170-176 (2018)
   PUBMED   29626470
  REMARK    GeneRIF: up regulation serum exosomal miR-675 and down regulation
            of CALN1 in tumor specimen was also found to be associated with the
            metastatic phenotype in osteosarcoma patients.
REFERENCE   4  (bases 1 to 9424)
  AUTHORS   Kobuke,K., Oki,K., Gomez-Sanchez,C.E., Gomez-Sanchez,E.P., Ohno,H.,
            Itcho,K., Yoshii,Y., Yoneda,M. and Hattori,N.
  TITLE     Calneuron 1 Increased Ca2+ in the Endoplasmic Reticulum and
            Aldosterone Production in Aldosterone-Producing Adenoma
  JOURNAL   Hypertension 71 (1), 125-133 (2018)
   PUBMED   29109191
  REMARK    GeneRIF: CALN1 gene encoding calneuron 1 had the strongest
            correlation with CYP11B2 (aldosterone synthase) among genes
            encoding Ca2+-binding proteins in Aldosterone-producing Adenoma.
REFERENCE   5  (bases 1 to 9424)
  AUTHORS   Ohi,K., Shimada,T., Nitta,Y., Kihara,H., Okubo,H., Uehara,T. and
            Kawasaki,Y.
  TITLE     Schizophrenia risk variants in ITIH4 and CALN1 regulate gene
            expression in the dorsolateral prefrontal cortex
  JOURNAL   Psychiatr Genet 26 (3), 142-143 (2016)
   PUBMED   26991396
  REMARK    GeneRIF: this report has further supported for associations of
            genetic variants in the ITIH4 and CALN1 genes with schizophrenia
            and provided the first evidence that the variants regulate ITIH4
            AND CALN1 expression in the dorsolateral prefrontal cortex
REFERENCE   6  (bases 1 to 9424)
  AUTHORS   Wilk,J.B., Shrine,N.R., Loehr,L.R., Zhao,J.H., Manichaikul,A.,
            Lopez,L.M., Smith,A.V., Heckbert,S.R., Smolonska,J., Tang,W.,
            Loth,D.W., Curjuric,I., Hui,J., Cho,M.H., Latourelle,J.C.,
            Henry,A.P., Aldrich,M., Bakke,P., Beaty,T.H., Bentley,A.R.,
            Borecki,I.B., Brusselle,G.G., Burkart,K.M., Chen,T.H., Couper,D.,
            Crapo,J.D., Davies,G., Dupuis,J., Franceschini,N., Gulsvik,A.,
            Hancock,D.B., Harris,T.B., Hofman,A., Imboden,M., James,A.L.,
            Khaw,K.T., Lahousse,L., Launer,L.J., Litonjua,A., Liu,Y.,
            Lohman,K.K., Lomas,D.A., Lumley,T., Marciante,K.D., McArdle,W.L.,
            Meibohm,B., Morrison,A.C., Musk,A.W., Myers,R.H., North,K.E.,
            Postma,D.S., Psaty,B.M., Rich,S.S., Rivadeneira,F., Rochat,T.,
            Rotter,J.I., Soler Artigas,M., Starr,J.M., Uitterlinden,A.G.,
            Wareham,N.J., Wijmenga,C., Zanen,P., Province,M.A., Silverman,E.K.,
            Deary,I.J., Palmer,L.J., Cassano,P.A., Gudnason,V., Barr,R.G.,
            Loos,R.J., Strachan,D.P., London,S.J., Boezen,H.M.,
            Probst-Hensch,N., Gharib,S.A., Hall,I.P., O'Connor,G.T., Tobin,M.D.
            and Stricker,B.H.
  TITLE     Genome-wide association studies identify CHRNA5/3 and HTR4 in the
            development of airflow obstruction
  JOURNAL   Am J Respir Crit Care Med 186 (7), 622-632 (2012)
   PUBMED   22837378
REFERENCE   7  (bases 1 to 9424)
  AUTHORS   Siedlinski,M., Cho,M.H., Bakke,P., Gulsvik,A., Lomas,D.A.,
            Anderson,W., Kong,X., Rennard,S.I., Beaty,T.H., Hokanson,J.E.,
            Crapo,J.D. and Silverman,E.K.
  CONSRTM   COPDGene Investigators; ECLIPSE Investigators
  TITLE     Genome-wide association study of smoking behaviours in patients
            with COPD
  JOURNAL   Thorax 66 (10), 894-902 (2011)
   PUBMED   21685187
REFERENCE   8  (bases 1 to 9424)
  CONSRTM   Schizophrenia Psychiatric Genome-Wide Association Study (GWAS)
            Consortium
  TITLE     Genome-wide association study identifies five new schizophrenia
            loci
  JOURNAL   Nat Genet 43 (10), 969-976 (2011)
   PUBMED   21926974
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 9424)
  AUTHORS   McCue,H.V., Burgoyne,R.D. and Haynes,L.P.
  TITLE     Membrane targeting of the EF-hand containing calcium-sensing
            proteins CaBP7 and CaBP8
  JOURNAL   Biochem Biophys Res Commun 380 (4), 825-831 (2009)
   PUBMED   19338761
  REMARK    GeneRIF: CaBP7 and 8 possess a targeting mechanism that is unique
            amongst the CaBPs that may contribute to differential functional
            Ca(2+)-sensing by these family members.
REFERENCE   10 (bases 1 to 9424)
  AUTHORS   Wu,Y.Q., Lin,X., Liu,C.M., Jamrich,M. and Shaffer,L.G.
  TITLE     Identification of a human brain-specific gene, calneuron 1, a new
            member of the calmodulin superfamily
  JOURNAL   Mol Genet Metab 72 (4), 343-350 (2001)
   PUBMED   11286509
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG718538.1, AF282250.1, DA805580.1, DA150825.1 and AL117636.1.
            
            On Nov 22, 2018 this sequence version replaced NM_031468.3.
            
            Summary: This gene encodes a protein with high similarity to the
            calcium-binding proteins of the calmodulin family. The encoded
            protein contains two EF-hand domains and potential calcium-binding
            sites. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: SRR1803612.94182.1, SRR1803612.137829.1
                                    [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA2151119 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395275.7/ ENSP00000378690.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-473               BG718538.1         43-515
            474-1710            AF282250.1         242-1478
            1711-5997           AF282250.1         1480-5766
            5998-6562           DA805580.1         5-569
            6563-6913           DA150825.1         179-529
            6914-9424           AL117636.1         9-2519
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q11.22"
     gene            1..9424
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /note="calneuron 1"
                     /db_xref="GeneID:83698"
                     /db_xref="HGNC:HGNC:13248"
                     /db_xref="MIM:607176"
     exon            1..281
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /inference="alignment:Splign:2.1.0"
     exon            282..473
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /inference="alignment:Splign:2.1.0"
     CDS             355..1140
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /note="isoform 1 is encoded by transcript variant 1;
                     calcium-binding protein 8; calcium-binding protein CABP8;
                     calneuron I"
                     /codon_start=1
                     /product="calcium-binding protein 8 isoform 1"
                     /protein_id="NP_113656.2"
                     /db_xref="CCDS:CCDS47603.1"
                     /db_xref="GeneID:83698"
                     /db_xref="HGNC:HGNC:13248"
                     /db_xref="MIM:607176"
                     /translation="MRLPEQPGEGKPENEKKGDGGALGGGEEPPRSQAPDFPTWEKMP
                     FHHVTAGLLYKGNYLNRSLSAGSDSEQLANISVEELDEIREAFRVLDRDGNGFISKQE
                     LGMAMRSLGYMPSEVELAIIMQRLDMDGDGQVDFDEFMTILGPKLVSSEGRDGFLGNT
                     IDSIFWQFDMQRITLEELKHILYHAFRDHLTMKDIENIIINEEESLNETSGNCQTEFE
                     GVHSQKQNRQTCVRKSLICAFAMAFIISVMLIAANQILRSGME"
     exon            474..598
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /inference="alignment:Splign:2.1.0"
     exon            599..742
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /inference="alignment:Splign:2.1.0"
     exon            743..855
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1012
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..9424
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1378..1383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /note="hexamer: AATAAA"
     polyA_site      1400
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /note="major polyA site"
     regulatory      2005..2010
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /note="hexamer: AATATA"
     polyA_site      2028
                     /gene="CALN1"
                     /gene_synonym="CABP8"
     regulatory      8169..8174
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /note="hexamer: AATAAA"
     polyA_site      8192
                     /gene="CALN1"
                     /gene_synonym="CABP8"
     regulatory      9401..9406
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALN1"
                     /gene_synonym="CABP8"
                     /note="hexamer: AATAAA"
     polyA_site      9424
                     /gene="CALN1"
                     /gene_synonym="CABP8"
ORIGIN      
        1 gaagctcgct ggggtgctat ccgcttgagt ttgtgttttt ttctttcctg cttcttaata
       61 aagcttactg cttactctgg gtccgcgcca cctttaaagc tcacggccgc ggtctgcgtc
      121 ttcgttcttg aagtcagcaa gaccgcaagc ccaccgggag gagcagacga ctccggacgc
      181 gccatcttta agagctgtaa cactcaccgc gaggtccgtg gttttattct taaagtcggc
      241 gagaccaaca acccagcaga aggaataaat tccgcacaca gagtctcagt gccttcgctg
      301 acgttccttt gatgagcttc taactctctc tgagaacatc gccctggacc cccgatgcgg
      361 ctgccagagc aacccggaga ggggaagccc gagaatgaga aaaaggggga cggaggagcc
      421 ctcggagggg gagaggagcc gccgaggagc caggcccccg acttccccac ctgggaaaag
      481 atgccgttcc accatgtgac cgccggcttg ttgtacaagg ggaattacct caaccgatcg
      541 ctctctgctg gcagtgacag cgaacagctg gctaatatct ccgtggagga gctcgatgaa
      601 atccgagagg cctttcgggt tctggaccgg gatgggaacg gcttcatctc caagcaggag
      661 ctgggcatgg ccatgcgctc tttggggtac atgccaagcg aggtggagct ggccatcatc
      721 atgcagcgct tggacatgga cggggatggc caggtggatt ttgatgaatt catgaccatt
      781 cttggcccca aactggtgtc ttcagaaggt cgcgatggtt ttcttgggaa cacgatagac
      841 agcatattct ggcagtttga catgcaaagg ataactctgg aagagttgaa gcacattctc
      901 tatcatgcct tccgagacca cctaacgatg aaggacattg agaacatcat tatcaatgag
      961 gaagagagcc tgaatgagac ctcggggaac tgccaaacag agtttgaagg agtgcattcc
     1021 cagaagcaga acagacagac ctgcgtccgg aagagcctca tatgcgcctt tgctatggcc
     1081 ttcatcatca gtgtcatgct gattgcagcc aaccagatac tccggagcgg catggagtag
     1141 cagcctcccg ccagccacac tgtgttgcag ctcaccgcgc atgtgcatgc cgtgcgggca
     1201 gactcttcct ccacacagca gacacggacc tatggactat ggatggatgc ggacgatgga
     1261 accgttcagt aaagaaccca aggttgagtg caactggggt tgcacttcag cttcatctcc
     1321 ttggcaggga cactaaaggg ctgtcttcag tgctttaatg gttttgtttt ctaatgcaat
     1381 aaatcgccag gatttccaaa ttattgcttc aaccatcagt cataactcta gagaaaaact
     1441 tcatcttcat ctcggaattc cagcagccat ccaggtataa tggtgagaga gcaagagaga
     1501 ggcatgaaca cacagtcacc tccactcccc attctttcaa cccaagggtc aacaggagac
     1561 tctctgctga ggtcaaccct cagttccaag aggctgactt cagggttgcc acagaagagg
     1621 gggctttcca tcccaccctg gcttccttcc tccacccagt ggagaccccc ccaaggcatg
     1681 aggggaaagc cattaagtgg ggtgcagccc tggcggtctt cagctgcacc cctcaccgct
     1741 tccgttctta ctgccttttc tagaggactg ccattggtag agctggggtg ctctcagttt
     1801 ccgtcggcac ttggctttct gttcctagag cccatcccgc accagcattt tggaacctgc
     1861 agagagcctt tctcccagct ttgccgcctg tgctgccatt agagaaaaaa agaagcataa
     1921 tcgtgggcat ctctataaca tagacagata tatatatttg gcttcttcgt gtgtgatgtc
     1981 attcttccct aatcgcttac acctaatata agcttgtcct ccctcccaca ctcatccctg
     2041 cttctttggg gcaggggtga ggcaggttga ggggggaggg gactgggact ggcggtgagg
     2101 ttttgatacc cacacccagc catctggagt catggagacc caccacgtct ctatgccgga
     2161 tgacctttgt aagttaaggt ccttgtgcat cttacctttc ccttaccttg gagtttgctt
     2221 tgttagtctt ccccagctaa aaagagaaat gcaatccaaa atggaaagat ggagagatca
     2281 aacgactgta ttttcacata agatcttctg gccttcagat tctgcaactg actcattttc
     2341 aggaggttgg gggaaaagat gtttatgagt ttagccaaca gaacaggtaa tttactcctg
     2401 cctgcagaat acttttcgtt ggggaagaga actgaatcca aggatctgtt ttcaacacca
     2461 ttccaagtct gtaatttcct gacttttcct agatgtcagc cactactatg aattcaagct
     2521 tgtgtagtca gattagccag taatctctag atgtaatagt tccctttgta cttttccaag
     2581 ctcaggtctt tattttgtaa tggaattacc ttgctcctac ttcttttgat tacatcctaa
     2641 aactacatgc ttcccccctt ccagtagcga gccaccagaa gctttcttgt ctctatgcag
     2701 tgggtaggat tttttggtgc gttgtgtgtt cctgatctcc ctccctgtct tcctgctcag
     2761 ctttctccct actgcctttt cagacttgga gcacccaagg tctccaatga aagttttctc
     2821 attcacctct gcaagtgcta ctggcaacag cctgagggca gctgtagaaa ctttccctct
     2881 gtgggagact atgaatattc ctgatgagca caagtaaaac taaggcttca gccataatat
     2941 ggaggaggga gcagatgtaa gctcccaaga gcctggggga tcacaggtcc ctctgcaaaa
     3001 cttcctggag ttcagcccag caaggccaga ccaaggaggc agcaacctgc ctttccccct
     3061 tacctgccct gctgcctgca cactgcatgc ctgccccttc tgctgctcct gttggctgac
     3121 tcaccactgt ccccttagga ttggaattct caactagaac cggtccaaat gcagaatatt
     3181 aatacatcga acaacttcat ccttaatctc tgccctgggc agtttgccct ctcccatctg
     3241 gacttagatg ggagggcata gcagatcttg aagggatata ctttcctgtt tttccctgac
     3301 ccccgaacca cccaaccaca tccatagggg tgcaggaccc taagtagact gggtagaaac
     3361 cccagggatg attcttcaaa gccccctcct ccatgagagg ggactgaaat gctgcagctg
     3421 aggctggtct tcagggcaga aaatgagtac ttctttgaag gtggcttttc tgcgagttct
     3481 tctgcctcct gctggagaac agagaccctt caagaggtga gggttagatt tcattggcag
     3541 agagattagc cactgtgcag gagtagggag gaaaaggagt tctggagagg cgccgccatc
     3601 ctgttttctg gaaaggctgc aggattcctt tcctaacggt gaccttcttc ttgctcccag
     3661 tgacctggaa gcaagcccac cctccccagg ggaaaatcta ggagaatgca ttcctctgca
     3721 gactgctaga gagagatggc aagaaggaag acacagaatt ctttctgaca gcctaggagt
     3781 tatcttccca ctgtggtaac cccccatgtg gcacccaggg actgaaggac acagtgcgtg
     3841 gagacggccc tgagctagcg gcattcctta gacttcagga gctcagagtt tgtcccggtg
     3901 acttgcaccc tggctgcttt gcttggacag tttgtcttgg gtatagggaa gccttccttg
     3961 tcaggcagcc aactcccagc cctgttcaca caaattttat gctagggccc catgccagtc
     4021 agtgtcccca tcatggtggc tatgaagctc acggctgaag attccagaag gtcctggtct
     4081 cccatgccca agggatgcca tgtagcccca gctggaagtg acttccgcag gaattgagag
     4141 ggaatagctc acccctcatt ctgtcctccg gacttaggaa ccagtgacct tggctgccca
     4201 actgtgtgga ggctgagtga ttgacaaaca caggttggct cattcttgct gcaagagcag
     4261 gtaccccccg ccgcccccct ggaaagcaca agtgatgagc aagaaggcat tttccttggg
     4321 agagaagggg aaatggaccc tgaccccctt gatccccaaa gaagctgcgc cccacctttc
     4381 cagataccca caaagccccc agacagaatc tagcatcggg actattagca atggcttcca
     4441 gaatgtgctt gggtcccacg tgactgatct gacaaaaaaa aaagagggat attaccatgg
     4501 caacccttgc attccaccct gagtttgcca aggcacacaa aaatagggaa cattttctta
     4561 aagaacactc caattcagcc tgagtgcaac ctgactctct gcatctgtat cctgctggga
     4621 gaggcaatta cccttaaaga atctgggtgg tatctctgcc tttttgtctg gcatttgaac
     4681 ttgtccttct atttactcag gagagcagga gctacctgta agccctggta tctccccatc
     4741 cctactccca gccccttctc ccagggtcca gagccaggac cccttaatct aggtaaattg
     4801 acagaaatat catcttggaa ggcaaacaag atattaagcc tccaatcttt aaaagtatcg
     4861 gtttgcctgt tgagcacact cctgcaagcc aaacacgtga actttggtga actgaggttt
     4921 aggtgtggtc ttaggcaaac ccagtggttt cagaaatgtc tgggtgtttt atttgcagag
     4981 aacaagatgt gtcttatgag tcttctttct caatacctgc cctgtctcaa atctcacttg
     5041 acaaatggtc tacgatcttg cagttatcca aaagagccta tgaaaaatgg acagctgctt
     5101 ttgacaataa tgcccctccc aacttcccat tcatagaatc ataaagcgat atgtttcaga
     5161 actgagagag aaaagtttac cttttattcc aaatgcctcg tactcggttt aagtccagac
     5221 tcaggtcata aatcaaagac agttttgcac gttgctcttc acctaaatct agcagtttcc
     5281 ctgaggccct atgagggcat ggacagaaaa tgaaggatgc aaccacccag gacagctccc
     5341 tggtttgggc tggcctggcc acgtgtggtc acatgtcctg ggatctgtgt ttactgtgtc
     5401 cttgctctcc ttccttgcag aagctgctaa gctctgctcc tcctaactgc aggtctcaaa
     5461 ccctattggg tcattttgcc acactatttc tccaaaggcc catagtcatt acatttttta
     5521 ccatttcacc aagataacag ggggggtctg gaattcccct gctaggaagg gccccttttc
     5581 ctatatcacc gtgatggtac agatgaactg agatgaaact tttagacttc agcacgtcac
     5641 acatcctggt tgtataacca ggaggtctgc agagctgcaa cccctgaaga acatctgtct
     5701 taaaagacct caaatcagaa cattctcatt ggcctcttca gtgatcccca tggagctaag
     5761 agtccgtaac ctaaaggcct taccatagcc atcttccctc cacacctgat tgctcaactg
     5821 ccccagaagg ggagaatcta tctgaaatag aaaagaagca ttaaggacca ggtgcggtgg
     5881 ctcacaccta taatcccagc actttgggag gccaaggcag gtggatcact taagatcagg
     5941 agttcaagac cagcctggcc aacgtggtga aaccccatct ctactaaaaa aaaaaaatac
     6001 aaaaattagc caagcatggt gtcgggcacc tgtaatccta gctactctgg aggctgagga
     6061 gggagaatca cttgaaccct ggaggcggag gctgcagtga gctgagatcg cgccactgca
     6121 ctccagcctg ggcaacagag caaaacttca tctcaaaaaa taattttaaa aaagaaacat
     6181 tattcaccag ctaagacttt tcaatttaaa ttatttaaag tgacagcaac atagaggaga
     6241 atttgggctg cctcttaccg acccagtctc catgataatt tgagtgtcac aactgttcag
     6301 cttcacttca tacttgcttt gactaagtca ctcgggattg tcctcaattt tttggccctg
     6361 ccataaatag gtgtatgtta gtccgttttg cattgctata aaggaatacc ttaacactgg
     6421 gtaatttata aagaaaagag gtttatttgg ctcgcagttc tgcaggctgt ttgagcaagg
     6481 tatcaacatc tgcttggctt ctggtgagac ctcagaaagc tttgacccat ggtggaaggt
     6541 gaagggggag caggcacgtc acacagcaag aacaggagca agagagagga gaagaggtgc
     6601 cagtctcctt ttcttaacaa cccgcaattg catgaactaa taaagtaaga attcactcat
     6661 tactacaggg agggcaccga gtcattcatg aggaatctgt ccctatgacc caaatatctc
     6721 ccagcaggcc ccacctccag cactggagat caacatgaaa tttgggggga acaaatatcc
     6781 agcctctatg attggtttgg agcaatgaga tcaattacac caacaagcca agcatttctc
     6841 ttagtatcct atggtcattg ctaatgcata tggtcatttt gacagctgtg gtttctcctt
     6901 catattcatt tgggactctt catagggtct cgagagacat gacaatactg ttcaaaacgt
     6961 ctccaaggca aaattaattt tattccgttg actggaaata tatcacaaag aatgaggttt
     7021 ttccagtccc acccacaaga cagtcctctg aaagtggaaa agcttgcccg gccacctgcc
     7081 cctaccctga ccttggggta gttcatcagt tgagcacaac ccccatgccg ggcatagact
     7141 cacccatctc ttttcctcag gtgtgatggg ttggctttca aactagtgtt tctcgcactg
     7201 ttagaagtta ttgtccaagt ggaaaggatg cagaggctgg aattttccag tgggatagaa
     7261 gagcaggcta attaggaagg aatattccag ggaatgcaag gtgcaactgc gatattgctc
     7321 catccctgga cctgagggag acaaagaaag ttcttaaagg gctgcagatg ctgtcctctg
     7381 aaggtcttct ctggatcgct ggcctacttt ggaaacatgc aggccattga gtgccatggg
     7441 gctgcccatc agtctgatcc acacgaaagg ctctgtgcat ttcaagagca ggctggtagc
     7501 tcgccattcc tgggaggatg ctgacatctg tcaagcccag aatgcctctc tcagggctct
     7561 cagacctgtt aaactgaggc caggagagca gaagaaagaa ctcaaagtgt ggacggtact
     7621 accagctgga gaaagcccag gagccaggac tcttccaccc actatctagg ttatgttgag
     7681 tgacattcct ccctggcttc aggtttctct aaagccagag gaaaagtaaa ccagacaagc
     7741 cctcccatct attttgtctg aaacaagatg gagtgaatgc tccctactga gagggtctgg
     7801 atttcaccca tgaattgcac taggaactgg gagctccttt ttgaacacaa gtcccaagag
     7861 gcagttgtga taaagtctct cttttggtgt ccgctgtctg taaatgaaac aatgcaaatt
     7921 ggtgtatgtt aaatgtgtca cagcttgcag gcaagaggtt ttgtttattc tctaaggact
     7981 tgctgactcg agggagcatc aaagatgagc tttctgtctg tcttggcaag tgtgaaaata
     8041 aagggagtca gtcacctccc caaaggtcat gtgaatcaga attcaggaga aagttggagg
     8101 aaacaatggc agattccaag aagagatgct tgtttctcca tttgagaaag ccttactgaa
     8161 agctactgaa taaagagcac aatttccagt tatttctttc tttgcttttt tttttttctg
     8221 gccactataa cagcacttcc agtatatgtt gatagtagct tgtaattatt cctaataccc
     8281 cagaaagaag agagtcgtga tctttggtga ttgtgaggaa ttagattaac agtcgcgggg
     8341 gaccggctac ctgcgacagc cctttaaatg gctaatgagg gatgctgagt gctgagcggt
     8401 gcctgctttt gatgtggccc acccatgacc agggagagag ggcaggaaga gagcagctgc
     8461 aggatccaca agtctctaac ccttctgccc caggcaacat aaaaccctag attcttgttc
     8521 tttttccctc ttcctaaagt agactgacgt cccagtaatt gtttttctaa caagccctgt
     8581 tgttctccct gggaaagacc tagattggcc aagaaaggat cctgtcagct cctggtggag
     8641 agttgcacaa gccagtggct ctcaactctc tccaaggaaa attaaagcat attttctctt
     8701 tcttagctca aaatcagctt tcaggggtcc ttattggggt ctgattttct tccccagttc
     8761 tgaatccagg aagaagcttt ttctccaagg accaaccaaa aacaatgact ttggagaaaa
     8821 attagactca gaaagtctgt ccaggcattg ggttggtgaa ctgtcaactg ttggatgggg
     8881 aggaggggtc gctgtgatcg tagcggtcat tagacttggg acgtgagttg tctctcaaag
     8941 cacagtagta aatgactgta ttttgtatat tgcccccaat atgttgggtg taaatagaag
     9001 cacagctggg gattgatcat ggccaggacg attggagctt ggcagctctc atatccagaa
     9061 taagccacta agacggaact catcaatcac catcttccca tttacctcat tagcatccca
     9121 ttttctctca ttcagtatta gttgatgatt tctgctatta agtgatgaga aagtcagcag
     9181 actagtcaaa aaagaatctt tatataaaat gtacatatta atataaatta acttagcatg
     9241 caacttaccg atcaaatgaa gtatatatta ttaatatgtt atcttagtgt ataaaggaaa
     9301 actgccatat ttaccagatg ttttctttaa ccgaacttgt ctgtaaatat ataatctgta
     9361 taaaaagagt ctaatttacc attatttatg caatagaaaa aataaaagtt tttttttctt
     9421 ttga
//
