LOCUS       NM_033429               3857 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens calmodulin like 4 (CALML4), transcript variant 1,
            mRNA.
ACCESSION   NM_033429 XM_370870
VERSION     NM_033429.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Choi,M.S., Graves,M.J., Matoo,S., Storad,Z.A., El Sheikh
            Idris,R.A., Weck,M.L., Smith,Z.B., Tyska,M.J. and Crawley,S.W.
  TITLE     The small EF-hand protein CALML4 functions as a critical myosin
            light chain within the intermicrovillar adhesion complex
  JOURNAL   J Biol Chem 295 (28), 9281-9296 (2020)
   PUBMED   32209652
  REMARK    GeneRIF: The small EF-hand protein CALML4 functions as a critical
            myosin light chain within the intermicrovillar adhesion complex.
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   GENDEP Investigators; MARS Investigators; STAR*D Investigators
  TITLE     Common genetic variation and antidepressant efficacy in major
            depressive disorder: a meta-analysis of three genome-wide
            pharmacogenetic studies
  JOURNAL   Am J Psychiatry 170 (2), 207-217 (2013)
   PUBMED   23377640
REFERENCE   3  (bases 1 to 3857)
  AUTHORS   Scanlan,M.J., Gout,I., Gordon,C.M., Williamson,B., Stockert,E.,
            Gure,A.O., Jager,D., Chen,Y.T., Mackay,A., O'Hare,M.J. and Old,L.J.
  TITLE     Humoral immunity to human breast cancer: antigen definition and
            quantitative analysis of mRNA expression
  JOURNAL   Cancer Immun 1, 4 (2001)
   PUBMED   12747765
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC107871.10.
            
            On Jul 27, 2020 this sequence version replaced NM_033429.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1034137.1,
                                           SRR11853562.11592.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2153946
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000467889.3/ ENSP00000419081.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                AC107871.10        93884-93971         c
            89-119              AC107871.10        93760-93790         c
            120-260             AC107871.10        88180-88320         c
            261-449             AC107871.10        86079-86267         c
            450-3857            AC107871.10        79344-82751         c
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q23"
     gene            1..3857
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="calmodulin like 4"
                     /db_xref="GeneID:91860"
                     /db_xref="HGNC:HGNC:18445"
                     /db_xref="MIM:620520"
     exon            1..88
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..547
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serologically defined breast cancer antigen NY-BR-20;
                     calmodulin-like protein 4"
                     /codon_start=1
                     /product="calmodulin-like protein 4 isoform 1"
                     /protein_id="NP_219501.3"
                     /db_xref="CCDS:CCDS10226.3"
                     /db_xref="GeneID:91860"
                     /db_xref="HGNC:HGNC:18445"
                     /db_xref="MIM:620520"
                     /translation="MAKFLSQDQINEYKECFSLYDKQQRGKIKATDLMVAMRCLGASP
                     TPGEVQRHLQTHGIDGNGELDFSTFLTIMHMQIKQEDPKKEILLAMLMVDKEKKGYVM
                     ASDLRSKLTSLGEKLTHKEVDDLFREADIEPNGKVKYDEFIHKITLPGRDY"
     exon            89..119
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /inference="alignment:Splign:2.1.0"
     exon            120..260
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /inference="alignment:Splign:2.1.0"
     exon            261..449
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /inference="alignment:Splign:2.1.0"
     exon            450..3857
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      733..738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="hexamer: AATAAA"
     polyA_site      760
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="major polyA site"
     regulatory      2483..2488
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="hexamer: AATAAA"
     polyA_site      2508
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
     regulatory      3453..3458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="hexamer: ATTAAA"
     polyA_site      3475
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
     regulatory      3502..3507
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="hexamer: AATAAT"
     polyA_site      3526
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
     regulatory      3733..3738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="hexamer: AGTAAA"
     polyA_site      3757
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
     regulatory      3833..3838
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
                     /note="hexamer: ATTAAA"
     polyA_site      3857
                     /gene="CALML4"
                     /gene_synonym="NY-BR-20"
ORIGIN      
        1 agactttaga cttgaggctg gaggaaaggg aactgaacgc ggttctggga gcagcaagcc
       61 cacgggtagc agccgaggcc ccagaatggc caagtttctt tcccaagacc aaattaatga
      121 gtacaaggaa tgcttctccc tgtatgacaa gcagcagagg gggaagataa aagccaccga
      181 cctcatggtg gccatgaggt gcctgggggc cagcccgacg ccaggggagg tgcagcggca
      241 cctgcagacc cacgggatag acggaaatgg agagctggat ttctccactt ttctgaccat
      301 tatgcacatg caaataaaac aagaagaccc aaagaaagaa attcttctag ccatgttgat
      361 ggtggacaag gagaagaaag gttacgtcat ggcgtccgac ctgcggtcaa aactcacgag
      421 tctgggggag aagctcaccc acaaggaagt ggatgatctc ttcagggaag cagatatcga
      481 acccaatggc aaagtgaagt atgatgaatt tatccacaag atcacccttc ctggacggga
      541 ctattgaagg aggagaatgg gagagcctcc cctgggcctg aaaacttgga gcaattaatt
      601 ttttttaaaa agtgttcttt tcacttggga gagatggcaa acacagtggc aagacaacat
      661 tacccaacta tagaagagag gctaactagc aacaataata gatgatttca gccatggtat
      721 gagtagatct ttaataaaag atttgtattg attttattaa ctaccgtgag tccggccctt
      781 tcaagcatgg aaggagcctg cggtttggag tctggcctgg gttccagtcc tggctctgct
      841 gcttcccact gtgactttgg gcaaatcatt tcactcctca aagccccccc acacaagctg
      901 gattcccact tcttacctca tggagcctgt tgaggaagga ttgagctgat gacttaaggg
      961 caatctacca agagacttat tctgtatttg ggggctagaa ccatcttcca tatttccaag
     1021 attttccaag atgaagccag tgctagctga gaagcagcaa tgaacagaaa gctgtaacac
     1081 ttatgacaac aattcttgca gtgccagagg cccatttaca aattctcatt tccatctcaa
     1141 cagatatagt gacatagctc aggctattca ttcataaaca cagagtgtag agtgaaaaca
     1201 ctagagtgaa aacacatgct acaatgaggc agcatcagct gagagcagga agagcgatct
     1261 actttacacc ccacaccaaa ggaaaccaga tgtgagctgc taaattgact ggccttgcag
     1321 agctcaagaa gggggcttcc aatgctgtga gaattccgag ctgttccctg ggctctgtta
     1381 acaggcagag aggttccggg atggtctgct caagtggccc acactggtca ttgccttaag
     1441 ccacctcccc aggacttacg gagagaaata aggggatgta accagcaatg gccagggtac
     1501 aacagccctg gaaaacagta gtaggagcac taggctttct gggagtccat ccagctggag
     1561 tggctttgag tgagttacac agctagaagg tgccaggttg gtgctgccag agattcagag
     1621 gtgccataca cttgtcaaat ctggatcatt cgtagtgcca gcacagtcct aaaagggctg
     1681 gagtaccaca ccaacacagg taggggtgca gggcttcaag tacaaagatt tgcatccatg
     1741 tatgtatcaa aagtgggttc tctgggctgt ggctttgtct agtagtacca cagtggctaa
     1801 agtagaagaa aaccaaatca aatgggatgt gtcttttggg aggatgtaca agacacaaat
     1861 ctttcactag gcaccgggca cagggaaaac tgcagggaac aagagttgta gtgttagtgc
     1921 aactgtctca acgatgctgt gtggcttcag acccaaacaa ggccctgagg aaggagactc
     1981 tcatttcccc aagcataact gcaaggagag gaggaattcc taggagccaa agagttttgt
     2041 ggggtgaggg taaataaatg gcccaaatgc caactaggtg aagttgtgac catctggctg
     2101 ggaagcccag gtccacacag tgtaggagca gatgttttgt ggggtctgag gtttacgaga
     2161 tttggctgcc ttaagaatac aaaaacagaa atgcagaatt tctggggctg ctcctaggac
     2221 cagaacaagt gaagggtcct ggtgcttaaa cttcattacc ttcatggtaa atccaccaga
     2281 gggccggtta gatgctggcc ccgccgagag aactgctgtc actttcaggc aaagctcaaa
     2341 ggtcctaggc ccacagttct tttgagctcc agtcatggac attaggaagt aaatcctgca
     2401 cagccaacct ggaataccaa agattagatg ggagatagat accaatgatt tagatggcac
     2461 aggaagagca agttctggat ataataaatg agggtacttt ccgtcaaagc ttttctatgt
     2521 ctatatttat cactgaatag tcccagtatg gttttaaagc aagttttatg aatctcattt
     2581 gcctaacagg aatctgaaat ataacttgcc aaaaacacac agttggtgtg gaatggtcat
     2641 tagaacctgg ggctcctctt cacggactcc ctgctcatta agggattcag tggtccagag
     2701 tctaagatcc tattaagtgt ttgattcaaa cctctacccg aggaagggct gttaccttac
     2761 tcctggtcct ggtttcaagc tcattcctga aattccagct ggtttctcta gcacctagtg
     2821 ttgtttacaa gaaggccacg gtgctcttag cattcaaact gcagatacta aacagatgct
     2881 gtgatttatt aaagagttag ccatatttca acaagaaagg gaaatgatgg ctatattcat
     2941 tacttacctc aaagcatgct gcaagaaaat tagttagtta cttgtcatgc tttgaaatct
     3001 ctggatgaaa ggtgctttgg aagcacaaac cattatcact tgtctcatag ggattgtccc
     3061 cttgaacatc cagcagtgtt attttacaga agacaaatta actgaaggct tttcttttat
     3121 tacatctaaa gagctctaca taaacaggta acattcaata ggtaaacaat ttttttccaa
     3181 tgcatgtaat aaatattttc acttggtact tttatacaaa ctgacattgt ctactataca
     3241 tttttaaaag ccattttact ggtttggcat gcggtatgga aattctaaga gagaaagttt
     3301 taaggcaatg aatcacagat ttaagttcat ggaatttatg gtaactttat ctgtttatgt
     3361 acattttccc ctttgttaaa caattaacag cagcacactc tgggaccacc agctattttc
     3421 cctctctttc tgaaatctaa gctttgtatt taattaaaaa acagaattca acatctattg
     3481 ataaaacaaa attcttacta aaataatttc aaatgtgctt taaaaagtcc tgaagatctt
     3541 gaaagtttta tgtgtttaaa attgaaattg tctaaaaaaa tgctctttcc acattaattt
     3601 agttaggata tattttcact ccatttcaga cacttgactc aaaggaaaat ctgccaaaga
     3661 atccgatttt tcagagctta cgtgaatctt tcctcagtaa agatacagaa ttgtgatcat
     3721 gtctaaataa ttagtaaagc aattttaatg ctcaaaatag tcaaccaagt atggcatggt
     3781 tctggttcag attttttttt tttaagatgt atccaataac actcacgaag taattaaaag
     3841 ccactttaac cctgcta
//
