LOCUS       NM_001363395            5651 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens calcium dependent secretion activator 2 (CADPS2),
            transcript variant 10, mRNA.
ACCESSION   NM_001363395 XM_017012795
VERSION     NM_001363395.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Andersen,J.S., Vijayakumaran,A., Godbehere,C., Lorentzen,E.,
            Mennella,V. and Schou,K.B.
  TITLE     Uncovering structural themes across cilia microtubule inner
            proteins with implications for human cilia function
  JOURNAL   Nat Commun 15 (1), 2687 (2024)
   PUBMED   38538594
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Yoo,H., Yang,S.H., Kim,J.Y., Yang,E., Park,H.S., Lee,S.J.,
            Rhyu,I.J., Turecki,G., Lee,H.W. and Kim,H.
  TITLE     Down-regulation of habenular calcium-dependent secretion activator
            2 induces despair-like behavior
  JOURNAL   Sci Rep 11 (1), 3700 (2021)
   PUBMED   33580180
  REMARK    GeneRIF: Down-regulation of habenular calcium-dependent secretion
            activator 2 induces despair-like behavior.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5651)
  AUTHORS   Zhang,K., Fan,Z., Wang,Y., Faraone,S.V., Yang,L. and Chang,S.
  TITLE     Genetic analysis for cognitive flexibility in the trail-making test
            in attention deficit hyperactivity disorder patients from single
            nucleotide polymorphism, gene to pathway level
  JOURNAL   World J Biol Psychiatry 20 (6), 476-485 (2019)
   PUBMED   28971736
  REMARK    GeneRIF: The top SNP rs2049161 involved gene DLGAP1 and the top
            gene CADPS2 in the gene-based analysis resulted in much literature
            evidence of associations with psychiatric disorders. Gene
            expression and network analysis showed their contribution to
            cognition function.
REFERENCE   4  (bases 1 to 5651)
  AUTHORS   Obergasteiger,J., Uberbacher,C., Pramstaller,P.P., Hicks,A.A.,
            Corti,C. and Volta,M.
  TITLE     CADPS2 gene expression is oppositely regulated by LRRK2 and
            alpha-synuclein
  JOURNAL   Biochem Biophys Res Commun 490 (3), 876-881 (2017)
   PUBMED   28647363
  REMARK    GeneRIF: These results indicate that LRRK2 and alpha-synuclein
            participate in the dysregulation of CADPS2 by altering
            transcription and support the hypothesis that synaptic
            dysfunctions, through different mechanisms, might contribute to the
            neuronal defects of diseases such as Parkinson's disease.
REFERENCE   5  (bases 1 to 5651)
  AUTHORS   Perry,J.R., Day,F., Elks,C.E., Sulem,P., Thompson,D.J.,
            Ferreira,T., He,C., Chasman,D.I., Esko,T., Thorleifsson,G.,
            Albrecht,E., Ang,W.Q., Corre,T., Cousminer,D.L., Feenstra,B.,
            Franceschini,N., Ganna,A., Johnson,A.D., Kjellqvist,S.,
            Lunetta,K.L., McMahon,G., Nolte,I.M., Paternoster,L., Porcu,E.,
            Smith,A.V., Stolk,L., Teumer,A., Tsernikova,N., Tikkanen,E.,
            Ulivi,S., Wagner,E.K., Amin,N., Bierut,L.J., Byrne,E.M.,
            Hottenga,J.J., Koller,D.L., Mangino,M., Pers,T.H.,
            Yerges-Armstrong,L.M., Zhao,J.H., Andrulis,I.L., Anton-Culver,H.,
            Atsma,F., Bandinelli,S., Beckmann,M.W., Benitez,J., Blomqvist,C.,
            Bojesen,S.E., Bolla,M.K., Bonanni,B., Brauch,H., Brenner,H.,
            Buring,J.E., Chang-Claude,J., Chanock,S., Chen,J.,
            Chenevix-Trench,G., Collee,J.M., Couch,F.J., Couper,D.,
            Coveillo,A.D., Cox,A., Czene,K., D'adamo,A.P., Smith,G.D., De
            Vivo,I., Demerath,E.W., Dennis,J., Devilee,P., Dieffenbach,A.K.,
            Dunning,A.M., Eiriksdottir,G., Eriksson,J.G., Fasching,P.A.,
            Ferrucci,L., Flesch-Janys,D., Flyger,H., Foroud,T., Franke,L.,
            Garcia,M.E., Garcia-Closas,M., Geller,F., de Geus,E.E., Giles,G.G.,
            Gudbjartsson,D.F., Gudnason,V., Guenel,P., Guo,S., Hall,P.,
            Hamann,U., Haring,R., Hartman,C.A., Heath,A.C., Hofman,A.,
            Hooning,M.J., Hopper,J.L., Hu,F.B., Hunter,D.J., Karasik,D.,
            Kiel,D.P., Knight,J.A., Kosma,V.M., Kutalik,Z., Lai,S.,
            Lambrechts,D., Lindblom,A., Magi,R., Magnusson,P.K., Mannermaa,A.,
            Martin,N.G., Masson,G., McArdle,P.F., McArdle,W.L., Melbye,M.,
            Michailidou,K., Mihailov,E., Milani,L., Milne,R.L., Nevanlinna,H.,
            Neven,P., Nohr,E.A., Oldehinkel,A.J., Oostra,B.A., Palotie,A.,
            Peacock,M., Pedersen,N.L., Peterlongo,P., Peto,J., Pharoah,P.D.,
            Postma,D.S., Pouta,A., Pylkas,K., Radice,P., Ring,S.,
            Rivadeneira,F., Robino,A., Rose,L.M., Rudolph,A., Salomaa,V.,
            Sanna,S., Schlessinger,D., Schmidt,M.K., Southey,M.C., Sovio,U.,
            Stampfer,M.J., Stockl,D., Storniolo,A.M., Timpson,N.J., Tyrer,J.,
            Visser,J.A., Vollenweider,P., Volzke,H., Waeber,G.,
            Waldenberger,M., Wallaschofski,H., Wang,Q., Willemsen,G.,
            Winqvist,R., Wolffenbuttel,B.H., Wright,M.J., Boomsma,D.I.,
            Econs,M.J., Khaw,K.T., Loos,R.J., McCarthy,M.I., Montgomery,G.W.,
            Rice,J.P., Streeten,E.A., Thorsteinsdottir,U., van Duijn,C.M.,
            Alizadeh,B.Z., Bergmann,S., Boerwinkle,E., Boyd,H.A., Crisponi,L.,
            Gasparini,P., Gieger,C., Harris,T.B., Ingelsson,E., Jarvelin,M.R.,
            Kraft,P., Lawlor,D., Metspalu,A., Pennell,C.E., Ridker,P.M.,
            Snieder,H., Sorensen,T.I., Spector,T.D., Strachan,D.P.,
            Uitterlinden,A.G., Wareham,N.J., Widen,E., Zygmunt,M., Murray,A.,
            Easton,D.F., Stefansson,K., Murabito,J.M. and Ong,K.K.
  CONSRTM   Australian Ovarian Cancer Study; GENICA Network; kConFab; LifeLines
            Cohort Study; InterAct Consortium; Early Growth Genetics (EGG)
            Consortium
  TITLE     Parent-of-origin-specific allelic associations among 106 genomic
            loci for age at menarche
  JOURNAL   Nature 514 (7520), 92-97 (2014)
   PUBMED   25231870
REFERENCE   6  (bases 1 to 5651)
  AUTHORS   Brunk,I., Blex,C., Speidel,D., Brose,N. and Ahnert-Hilger,G.
  TITLE     Ca2+-dependent activator proteins of secretion promote vesicular
            monoamine uptake
  JOURNAL   J Biol Chem 284 (2), 1050-1056 (2009)
   PUBMED   19008227
  REMARK    GeneRIF: CAPS proteins are involved in optimizing vesicular
            monoamine uptake and storage mediated by VMAT1 and VMAT2
REFERENCE   7  (bases 1 to 5651)
  AUTHORS   Sadakata,T., Washida,M., Iwayama,Y., Shoji,S., Sato,Y., Ohkura,T.,
            Katoh-Semba,R., Nakajima,M., Sekine,Y., Tanaka,M., Nakamura,K.,
            Iwata,Y., Tsuchiya,K.J., Mori,N., Detera-Wadleigh,S.D.,
            Ichikawa,H., Itohara,S., Yoshikawa,T. and Furuichi,T.
  TITLE     Autistic-like phenotypes in Cadps2-knockout mice and aberrant
            CADPS2 splicing in autistic patients
  JOURNAL   J Clin Invest 117 (4), 931-943 (2007)
   PUBMED   17380209
  REMARK    GeneRIF: Genetic disturbance in CADPS2-mediated neurotrophin
            release contributes to autism susceptibility.
            GeneRIF: CADPS2-KO mice show autistic-like phenotypes. Moreover,
            the results show that some autistic patients have an aberrant
            splicing variant of CADPS2 mRNA, suggesting that a disturbance in
            CADPS2-mediated neurotrophin release contributes to autism.
            GeneRIF: Results from Cadps2-deficient mice and human data suggest
            that a disturbance in CADPS2-mediated neurotrophin release
            contributes to autistic-like cellular and behavioral phenotypes.
REFERENCE   8  (bases 1 to 5651)
  AUTHORS   Kim,J.M., Lee,K.H., Jeon,Y.J., Oh,J.H., Jeong,S.Y., Song,I.S.,
            Kim,J.M., Lee,D.S. and Kim,N.S.
  TITLE     Identification of genes related to Parkinson's disease using
            expressed sequence tags
  JOURNAL   DNA Res 13 (6), 275-286 (2006)
   PUBMED   17213182
REFERENCE   9  (bases 1 to 5651)
  AUTHORS   Speidel,D., Varoqueaux,F., Enk,C., Nojiri,M., Grishanin,R.N.,
            Martin,T.F., Hofmann,K., Brose,N. and Reim,K.
  TITLE     A family of Ca2+-dependent activator proteins for secretion:
            comparative analysis of structure, expression, localization, and
            function
  JOURNAL   J Biol Chem 278 (52), 52802-52809 (2003)
   PUBMED   14530279
  REMARK    GeneRIF: identification, cloning, and comparative characterization
            of a second mammalian CAPS isoform, CAPS2; concluded that at the
            functional level, CAPS2 is largely redundant with CAPS1
REFERENCE   10 (bases 1 to 5651)
  AUTHORS   Cisternas,F.A., Vincent,J.B., Scherer,S.W. and Ray,P.N.
  TITLE     Cloning and characterization of human CADPS and CADPS2, new members
            of the Ca2+-dependent activator for secretion protein family
  JOURNAL   Genomics 81 (3), 279-291 (2003)
   PUBMED   12659812
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004838.2, AC006463.4,
            AC091438.4, AC006009.2, AC004594.2 and AC015983.7.
            
            On Aug 14, 2020 this sequence version replaced NM_001363395.1.
            
            Summary: This gene encodes a member of the calcium-dependent
            activator of secretion (CAPS) protein family, which are calcium
            binding proteins that regulate the exocytosis of synaptic and
            dense-core vesicles in neurons and neuroendocrine cells. Mutations
            in this gene may contribute to autism susceptibility. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Nov 2009].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.985063.1,
                                           SRR11853560.26615.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-462               AC004838.2         120142-120603
            463-576             AC006463.4         119220-119333       c
            577-909             AC006463.4         45502-45834         c
            910-990             AC006463.4         11513-11593         c
            991-1227            AC006463.4         3746-3982           c
            1228-1346           AC091438.4         28833-28951         c
            1347-1458           AC006009.2         88573-88684         c
            1459-1598           AC006009.2         61944-62083         c
            1599-1665           AC006009.2         20643-20709         c
            1666-1774           AC004594.2         150380-150488       c
            1775-1975           AC004594.2         149149-149349       c
            1976-2112           AC004594.2         133449-133585       c
            2113-2300           AC004594.2         130443-130630       c
            2301-2402           AC004594.2         110442-110543       c
            2403-2466           AC004594.2         100580-100643       c
            2467-2590           AC004594.2         97409-97532         c
            2591-2611           AC004594.2         95428-95448         c
            2612-2715           AC004594.2         75129-75232         c
            2716-2872           AC004594.2         66608-66764         c
            2873-3014           AC004594.2         52509-52650         c
            3015-3170           AC004594.2         47651-47806         c
            3171-3318           AC004594.2         46094-46241         c
            3319-3393           AC004594.2         38436-38510         c
            3394-3477           AC004594.2         19998-20081         c
            3478-3510           AC004594.2         19856-19888         c
            3511-3618           AC004594.2         4642-4749           c
            3619-3723           AC015983.7         161821-161925       c
            3724-5651           AC015983.7         154755-156682       c
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q31.32"
     gene            1..5651
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /note="calcium dependent secretion activator 2"
                     /db_xref="GeneID:93664"
                     /db_xref="HGNC:HGNC:16018"
                     /db_xref="MIM:609978"
     exon            1..462
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..3897
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /note="isoform j is encoded by transcript variant 10;
                     Ca2+-dependent activator protein for secretion 2; Ca2+
                     dependent secretion activator 2; CAPS-2; calcium-dependent
                     activator protein for secretion 2; Ca++-dependent
                     secretion activator 2"
                     /codon_start=1
                     /product="calcium-dependent secretion activator 2 isoform
                     j"
                     /protein_id="NP_001350324.1"
                     /db_xref="GeneID:93664"
                     /db_xref="HGNC:HGNC:16018"
                     /db_xref="MIM:609978"
                     /translation="MLDPSSSEEESDEGLEEESRDVLVAAGSSQRAPPAPTREGRRDA
                     PGRAGGGGAARSVSPSPSVLSEGRDEPQRQLDDEQERRIRLQLYVFVVRCIAYPFNAK
                     QPTDMARRQQKLNKQQLQLLKERFQAFLNGETQIVADEAFCNAVRSYYEVFLKSDRVA
                     RMVQSGGCSANDFREVFKKNIEKRVRSLPEIDGLSKETVLSSWIAKYDAIYRGEEDLC
                     KQPNRMALSAVSELILSKEQLYEMFQQILGIKKLEHQLLYNACQLDNADEQAAQIRRE
                     LDGRLQLADKMAKERKFPKFIAKDMENMYIEELRSSVNLLMANLESLPVSKGGPEFKL
                     QKLKRSQNSAFLDIGDENEIQLSKSDVVLSFTLEIVIMEVQGLKSVAPNRIVYCTMEV
                     EGEKLQTDQAEASRPQWGTQGDFTTTHPRPVVKVKLFTESTGVLALEDKELGRVILYP
                     TSNSSKSAELHRMVVPKNSQDSDLKIKLAVRMDKPAHMKHSGYLYALGQKVWKRWKKR
                     YFVLVQVSQYTFAMCSYREKKSEPQELMQLEGYTVDYTDPHPGLQGGCMFFNAVKEGD
                     TVIFASDDEQDRILWVQAMYRATGQSYKPVPAIQTQKLNPKGGTLHADAQLYADRFQK
                     HGMDEFISANPCKLDHAFLFRILQRQTLDHRLNDSYSCLGWFSPGQVFVLDEYCARYG
                     VRGCHRHLCYLAELMEHSENGAVIDPTLLHYSFAFCASHVHGNRPDGIGTVSVEEKER
                     FEEIKERLSSLLENQISHFRYCFPFGRPEGALKATLSLLERVLMKDIATPIPAEEVKK
                     VVRKCLEKAALINYTRLTEYAKIEGPAEKETETMNQASPARKLEEILHLAELCIEVLQ
                     QNEEHHAEAFAWWPDLLAEHAEKFWALFTVDMDTALEAQPQDSWDSFPLFQLLNNFLR
                     NDTLLCNGKFHKHLQEIFVPLVVRYVDLMESSIAQSIHRGFEQETWQPVNNGSATSED
                     LFWKLDALQMFVFDLHWPEQEFAHHLEQRLKLMASDMLEACVKRTRTAFELKLQKASK
                     TTDLRIPASVCTMFNVLVDAKKQSTKLCALDGGQEQQYHSKIDDLIDNSVKEIISLLV
                     SKFVSVLEGVLSKLSRYDEGTFFSSILSFTVKAAAKYVDVPKPGMDLADTYIMFVRQN
                     QDILREKVNEEMYIEKLFDQWYSSSMKVICVWLTDRLDLQLHIYQLKTLIKIVKKTYR
                     DFRLQGVLEGTLNSKTYDTVHRRLTVEEATASVSEGGGLQGITMKDSDEEEEG"
     exon            463..576
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            577..909
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            910..990
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1227
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1346
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1458
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1598
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1665
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1774
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1975
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2112
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2113..2300
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2402
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2466
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..2590
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..2611
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2612..2715
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2872
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2873..3014
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3170
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3171..3318
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3319..3393
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3394..3477
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3478..3510
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3511..3618
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3619..3723
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3724..5651
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4643..4648
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /note="hexamer: AATAAA"
     polyA_site      4665
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /note="major polyA site"
     regulatory      4704..4709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /note="hexamer: AGTAAA"
     polyA_site      4738
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
     regulatory      4813..4818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /note="hexamer: AGTAAA"
     polyA_site      4833
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
     regulatory      5613..5618
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
                     /note="hexamer: AATAAA"
     polyA_site      5651
                     /gene="CADPS2"
                     /gene_synonym="CAPS2"
ORIGIN      
        1 agttttcctt tcgttctgcg gccgctgcag ccagccccgc ggctccctca gacccgcggg
       61 cgcagccgcc gggggtgagg cgcttgggga ccgcgggccg agcggcgggg atccccgagc
      121 accatgctgg acccgtcttc cagcgaagag gagtcggacg aggggctgga agaggaaagc
      181 cgcgatgtgc tggtggcagc cggcagctcg cagcgagctc ctccagcccc gactcgggaa
      241 gggcggcggg acgcgccggg gcgcgcgggc ggcggcggcg cggccagatc tgtgagcccg
      301 agcccctctg tgctcagcga ggggcgagac gagccccagc ggcagctgga cgatgagcag
      361 gagcggagga tccgcctgca gctctacgtc ttcgtcgtga ggtgcatcgc gtaccccttc
      421 aacgccaagc agcccaccga catggcccgg aggcagcaga agcttaacaa acaacagttg
      481 cagttactga aagaacggtt ccaggccttc ctcaatgggg aaacccaaat tgtagctgac
      541 gaagcatttt gcaacgcagt tcggagttat tatgaggttt ttctaaagag tgaccgagtg
      601 gccagaatgg tacagagtgg agggtgttct gctaatgact tcagagaagt atttaagaaa
      661 aacatagaaa aacgtgtgcg gagtttgcca gaaatagatg gcttgagcaa agagacagtg
      721 ttgagctcat ggatagccaa atatgatgcc atttacagag gtgaagagga cttgtgcaaa
      781 cagccaaata gaatggccct aagtgcagtg tctgaactta ttctgagcaa ggaacaactc
      841 tatgaaatgt ttcagcagat tctgggtatt aaaaaactgg aacaccagct cctttataat
      901 gcatgtcagc tggataacgc agatgaacaa gcagcccaga tcagaaggga acttgatggc
      961 cggctgcaat tggcagataa aatggcaaag gaaagaaaat tccccaaatt tatagcaaaa
     1021 gatatggaga atatgtatat agaagagttg cggtcttcag tgaatttgct aatggccaat
     1081 ttggaaagtc ttccagtttc gaaaggtggt ccggaattta aattacaaaa attaaaacgt
     1141 tcacagaact ctgcattttt ggacatagga gatgagaatg agattcagct gtcaaagtcc
     1201 gacgtggtac tgtcattcac cttagagatt gtcataatgg aagtgcaagg cctgaagtca
     1261 gttgctccca atcgaattgt ttactgtaca atggaagtgg aaggagaaaa acttcagaca
     1321 gaccaggccg aagcctcaag gccacaatgg gggactcaag gagatttcac caccacccat
     1381 cctcggcctg tggtcaaagt gaaactcttc acagaaagca ctggagttct ggccctggaa
     1441 gataaagaac tgggaagggt gatattatac ccaacttcta atagctccaa atcagctgaa
     1501 ttacaccgaa tggtagttcc aaaaaatagc caggattctg acttaaaaat caaactggca
     1561 gtgcgaatgg ataaaccagc acatatgaag catagtggat atctgtatgc ccttggacag
     1621 aaggtttgga aaagatggaa aaaacgttac tttgttctag ttcaggttag ccaatatacc
     1681 tttgctatgt gcagttatag agaaaagaag tctgaaccac aagaattaat gcagcttgaa
     1741 ggctatactg tggattatac cgatccccac ccaggccttc agggtggttg tatgttcttt
     1801 aatgctgtta aagaaggaga tactgtaatc tttgccagtg atgatgaaca ggacagaata
     1861 ttatgggttc aagccatgta tagggccaca ggtcaatcat ataaaccagt tcctgcaatt
     1921 caaacccaga aactgaatcc taaaggagga actctccatg cagatgctca gctttatgca
     1981 gatcgttttc agaaacatgg tatggatgag tttatttctg caaacccctg caagcttgat
     2041 catgccttcc tttttagaat actccagagg cagactttgg atcacagact gaatgattcc
     2101 tattcttgct tgggatggtt tagccctggc caagtctttg tgttagatga gtactgtgcc
     2161 cgttatggtg tgagaggctg tcacagacat ctctgctacc ttgcagaact gatggaacat
     2221 tcagaaaatg gtgctgtcat tgaccctacc ctgctccatt acagctttgc attctgtgcc
     2281 tctcatgtgc acggcaacag gcctgatgga attgggactg tttcagtgga agaaaaagaa
     2341 agatttgagg agataaaaga gagactctct tcccttttag aaaatcagat aagccatttc
     2401 agatactgtt ttccctttgg acgacctgaa ggtgctctaa aagctacact ttcattactt
     2461 gaaagggttt taatgaaaga tattgccact cccataccag cagaagaggt gaagaaagtg
     2521 gtcagaaaat gtctcgagaa agctgccttg atcaattaca ctagactcac agaatatgcc
     2581 aaaatagaag gcccagcaga aaaggaaaca gagaccatga accaggcatc tcctgctaga
     2641 aagctggaag agattcttca tctggcagag ctctgcatag aagtcttaca gcagaatgaa
     2701 gagcatcatg cagaggcatt tgcctggtgg cctgatttat tggctgaaca tgcagagaaa
     2761 ttttgggctt tatttacagt ggatatggac actgcactag aggctcaacc gcaagactcc
     2821 tgggatagtt ttcctctttt ccaactgctt aataatttcc tccgaaatga cacacttttg
     2881 tgtaatggaa aatttcacaa acacttgcaa gaaatctttg tacccttggt tgtccgctat
     2941 gtggatctca tggagtcttc catcgcccag tcaattcaca gaggttttga gcaggagaca
     3001 tggcagcctg tcaacaatgg ctcagcaaca tcagaagacc ttttttggaa gcttgatgca
     3061 ctgcaaatgt ttgtctttga tctgcactgg ccagaacagg aatttgccca ccacttagag
     3121 caaagactta aactaatggc cagtgatatg ctagaggcct gtgtcaaaag aacaagaact
     3181 gcatttgaac tcaagctaca aaaggcaagc aaaacaactg acttgcgcat tccagcttcc
     3241 gtttgcacta tgtttaatgt attagtcgat gccaaaaagc aaagcaccaa actctgtgcc
     3301 ctggatggag gacaagagca acagtaccat tcaaaaatag atgatctgat cgacaacagt
     3361 gtaaaagaaa tcatttcact gttagtttca aagtttgttt cagtgttgga aggcgtgttg
     3421 tctaagctgt caaggtatga tgaaggcact ttcttttcat ccattctgtc attcactgtg
     3481 aaagcagctg caaaatatgt tgatgttcca aaaccaggaa tggatctggc agacacctat
     3541 attatgtttg ttcggcaaaa ccaagatatt cttcgagaaa aggtcaatga ggaaatgtat
     3601 atagaaaagt tatttgatca atggtacagc agttccatga aagtcatttg cgtgtggttg
     3661 actgatagat tagacctcca actccatatt taccagctga agacgctcat caagattgtg
     3721 aagaaaacct acagggactt tcgattgcag ggtgtgttgg aaggaacact gaacagtaag
     3781 acttatgata ctgtgcacag acgtttaaca gtagaggagg ccacagcctc tgtttcagaa
     3841 ggaggaggac ttcagggcat tactatgaaa gacagtgacg aagaagaaga aggctgatat
     3901 cacacagctt tgcagaagga aggaagacct tgatcgacat tgttttttat ttttttaacc
     3961 ttgtccttgt aattacattc attgtttgtt ttggccaaat aaaaatgctt gtatttcttt
     4021 aaaaagtaag cctgaatgta gagtaaaagg ggaaatgcca agattttggg gtttttttgt
     4081 ttcctttttt tgtttgtttg tttgtttgtt tttttggaga agagcatcct cttttgtgta
     4141 gtttgaccta aaaatgaacc ttggctctgc ttgtgatcag aacatgaact ttttttttta
     4201 aagaagattt gagcattttt ctgtaatcac atcaaaatga tgttttctgt gtaaagcgag
     4261 atacatattt ctcataatgc agcattgtga gaagtcagtt cggaccactg caccaacact
     4321 gtcgtatcct tgttaaaatg gtgtgtacct tacaaattat aatttatgtg ccaggttcgt
     4381 tttgtactta atttgctatt attgtgatgt gtataaaatc tttaatcttg gttcttagta
     4441 ctttgaattg gtctacaggt atattcctgg gatgaaagga ttgccaaacc caaatataga
     4501 ctagattatc caatgggttt gtgtctttgt tccattctca acatttcttc tttcaactat
     4561 aagtaatccc caggtgtggg gtagcaagtg tgcttccgtc aagataccat attctcctgc
     4621 tccagtataa cagcttgcag gcaataaaaa tctatttgct cataactact tctgtattta
     4681 ttagacttat atagagcaaa tgcagtaaaa gaggtttgca gtgtttcaaa catcccaact
     4741 atctgactct gtgtttttgt ttcttgagtt ttttgtttgt tttgtttgta atattttgtt
     4801 tttaatcaaa tgagtaaaag atagtaacac tgactgttct ttgtacctaa taggcacata
     4861 cattgttaaa agaattacta tgacagcata tttccatagc agaggaaata tatatttcat
     4921 atattattag ttaatattag taatcaatgt taactaataa ttagtaatgt tagtaatatt
     4981 aactagtaat tcacattatt agtggttctc tcaaaatatt ttcttgctag ggtttgagag
     5041 ataattgtat tatgttgcgt aagttctcta tgaattattt ttgtgatatt tcccaaaaca
     5101 tattggcaga ttgtgctact gcatttatta ataagtaatt tttaaatgaa ggcagttaat
     5161 gcagaataag tggaggtatc cctgcattta tgaaaacgtt tattctgaaa ctttagaaat
     5221 gaaacaactt ggacctccag attcgttctc ccatggtgta ataagtggtc gttaggtacc
     5281 tagactagcc ttaaaaatct atttaatcta cagttactat ttagcattgt tttggctcat
     5341 tttctgggcc ctagggctaa gataacaaac aaggaaattt cttccttctt ccgggattaa
     5401 agactaactc taccaactca ctatctttga aaggcagttt gggaatgttc tccctccttt
     5461 tcttctctat tcacacacca aatactattt tctccaaacc ctcaaatgtc cagtcatgct
     5521 atcccaaact ctccaatggc accatcccca agcttgctgt tccacgacac attgctctgt
     5581 acttagtaat tacccaccct tcttttcctt tcaataaaat gtccatttcc ttgatacaga
     5641 tcatctccaa a
//
