LOCUS       NM_003716               5507 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens calcium dependent secretion activator (CADPS),
            transcript variant 1, mRNA.
ACCESSION   NM_003716
VERSION     NM_003716.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Sitbon,J., Nestvogel,D., Kappeler,C., Nicolas,A., Maciuba,S.,
            Henrion,A., Troudet,R., Courtois,E., Grannec,G., Latapie,V.,
            Barau,C., Le Corvoisier,P., Pietrancosta,N., Henry,C., Leboyer,M.,
            Etain,B., Nosten-Bertrand,M., Martin,T.F.J., Rhee,J. and Jamain,S.
  TITLE     CADPS functional mutations in patients with bipolar disorder
            increase the sensitivity to stress
  JOURNAL   Mol Psychiatry 27 (2), 1145-1157 (2022)
   PUBMED   35169262
  REMARK    GeneRIF: CADPS functional mutations in patients with bipolar
            disorder increase the sensitivity to stress.
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Weng,S., Janssen,H.L.A., Zhang,N., Tang,W., Bai,E., Yang,B. and
            Dong,L.
  TITLE     CAPS1 Suppresses Tumorigenesis in Cholangiocarcinoma
  JOURNAL   Dig Dis Sci 65 (4), 1053-1063 (2020)
   PUBMED   31562609
  REMARK    GeneRIF: CAPS1 Suppresses Tumorigenesis in Cholangiocarcinoma.
REFERENCE   3  (bases 1 to 5507)
  AUTHORS   Wu,B., Sun,D., Ma,L., Deng,Y., Zhang,S., Dong,L. and Chen,S.
  TITLE     Exosomes isolated from CAPS1-overexpressing colorectal cancer cells
            promote cell migration
  JOURNAL   Oncol Rep 42 (6), 2528-2536 (2019)
   PUBMED   31638236
  REMARK    GeneRIF: Study revealed that CAPS1 promoted normal colonic
            epithelial FHC cells migration by altering the protein expression
            profile of exosomes derived from colorectal cancer cells cells.
            GW4869, an exosome inhibitor, inhibited CAPS1-induced cell
            migration.
REFERENCE   4  (bases 1 to 5507)
  AUTHORS   Zhao,G.X., Xu,Y.Y., Weng,S.Q., Zhang,S., Chen,Y., Shen,X.Z.,
            Dong,L. and Chen,S.
  TITLE     CAPS1 promotes colorectal cancer metastasis via Snail mediated
            epithelial mesenchymal transformation
  JOURNAL   Oncogene 38 (23), 4574-4589 (2019)
   PUBMED   30742066
  REMARK    GeneRIF: CAPS1 promotes colorectal cancer metastasis through
            PI3K/Akt/GSK3beta/Snail signal pathway-mediated epithelial
            mesenchymal transformation process
REFERENCE   5  (bases 1 to 5507)
  AUTHORS   Yu,B., Zheng,Y., Alexander,D., Manolio,T.A., Alonso,A.,
            Nettleton,J.A. and Boerwinkle,E.
  TITLE     Genome-wide association study of a heart failure related
            metabolomic profile among African Americans in the Atherosclerosis
            Risk in Communities (ARIC) study
  JOURNAL   Genet Epidemiol 37 (8), 840-845 (2013)
   PUBMED   23934736
REFERENCE   6  (bases 1 to 5507)
  AUTHORS   Berwin,B., Floor,E. and Martin,T.F.
  TITLE     CAPS (mammalian UNC-31) protein localizes to membranes involved in
            dense-core vesicle exocytosis
  JOURNAL   Neuron 21 (1), 137-145 (1998)
   PUBMED   9697858
REFERENCE   7  (bases 1 to 5507)
  AUTHORS   Loyet,K.M., Kowalchyk,J.A., Chaudhary,A., Chen,J., Prestwich,G.D.
            and Martin,T.F.
  TITLE     Specific binding of phosphatidylinositol 4,5-bisphosphate to
            calcium-dependent activator protein for secretion (CAPS), a
            potential phosphoinositide effector protein for regulated
            exocytosis
  JOURNAL   J Biol Chem 273 (14), 8337-8343 (1998)
   PUBMED   9525942
REFERENCE   8  (bases 1 to 5507)
  AUTHORS   Ann,K., Kowalchyk,J.A., Loyet,K.M. and Martin,T.F.
  TITLE     Novel Ca2+-binding protein (CAPS) related to UNC-31 required for
            Ca2+-activated exocytosis
  JOURNAL   J Biol Chem 272 (32), 19637-19640 (1997)
   PUBMED   9289490
REFERENCE   9  (bases 1 to 5507)
  AUTHORS   Nishizaki,T., Walent,J.H., Kowalchyk,J.A. and Martin,T.F.
  TITLE     A key role for a 145-kDa cytosolic protein in the stimulation of
            Ca(2+)-dependent secretion by protein kinase C
  JOURNAL   J Biol Chem 267 (33), 23972-23981 (1992)
   PUBMED   1429734
REFERENCE   10 (bases 1 to 5507)
  AUTHORS   Walent,J.H., Porter,B.W. and Martin,T.F.
  TITLE     A novel 145 kd brain cytosolic protein reconstitutes
            Ca(2+)-regulated secretion in permeable neuroendocrine cells
  JOURNAL   Cell 70 (5), 765-775 (1992)
   PUBMED   1516133
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012519.9, AC092039.2 and
            AC104161.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003716.3.
            
            Summary: This gene encodes a novel neural/endocrine-specific
            cytosolic and peripheral membrane protein required for the
            Ca2+-regulated exocytosis of secretory vesicles. The protein acts
            at a stage in exocytosis that follows ATP-dependent priming, which
            involves the essential synthesis of phosphatidylinositol
            4,5-bisphosphate (PtdIns(4,5)P2). Alternative splicing has been
            observed at this locus and three variants, encoding distinct
            isoforms, are described. [provided by RefSeq, Aug 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB032947.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000383710.9/ ENSP00000373215.4
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AC012519.9         161715-161741       c
            28-828              AC012519.9         160914-161714       c
            829-942             AC012519.9         52196-52309         c
            943-1275            AC012519.9         39766-40098         c
            1276-1356           AC092039.2         149784-149864       c
            1357-1590           AC092039.2         138317-138550       c
            1591-1712           AC092039.2         133192-133313       c
            1713-1824           AC092039.2         80107-80218         c
            1825-1964           AC092039.2         72655-72794         c
            1965-2031           AC092039.2         58342-58408         c
            2032-2140           AC092039.2         44875-44983         c
            2141-2353           AC092039.2         37373-37585         c
            2354-2490           AC104161.2         154501-154637       c
            2491-2678           AC104161.2         150927-151114       c
            2679-2780           AC104161.2         136205-136306       c
            2781-2844           AC104161.2         134636-134699       c
            2845-2968           AC104161.2         134115-134238       c
            2969-2986           AC104161.2         130807-130824       c
            2987-3093           AC104161.2         117218-117324       c
            3094-3114           AC104161.2         111701-111721       c
            3115-3271           AC104161.2         110346-110502       c
            3272-3413           AC104161.2         109395-109536       c
            3414-3560           AC104161.2         99779-99925         c
            3561-3716           AC104161.2         96317-96472         c
            3717-3864           AC104161.2         92229-92376         c
            3865-3939           AC104161.2         84395-84469         c
            3940-4023           AC104161.2         83423-83506         c
            4024-4056           AC104161.2         63821-63853         c
            4057-4164           AC104161.2         56160-56267         c
            4165-4269           AC104161.2         21137-21241         c
            4270-5507           AC104161.2         16404-17641         c
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p14.2"
     gene            1..5507
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /note="calcium dependent secretion activator"
                     /db_xref="GeneID:8618"
                     /db_xref="HGNC:HGNC:1426"
                     /db_xref="MIM:604667"
     exon            1..828
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     CDS             388..4449
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Ca2+-dependent activator protein for secretion;
                     Ca2+-regulated cytoskeletal protein; calcium-dependent
                     secretion activator 1; CAPS-1; calcium-dependent activator
                     protein for secretion 1; Ca++-dependent secretion
                     activator; Ca2+ dependent secretion activator"
                     /codon_start=1
                     /product="calcium-dependent secretion activator 1 isoform
                     1"
                     /protein_id="NP_003707.2"
                     /db_xref="CCDS:CCDS46858.1"
                     /db_xref="GeneID:8618"
                     /db_xref="HGNC:HGNC:1426"
                     /db_xref="MIM:604667"
                     /translation="MLDPSSSEEESDEIVEEESGKEVLGSAPSGARLSPSRTSEGSAG
                     SAGLGGGGAGAGAGVGAGGGGGSGASSGGGAGGLQPSSRAGGGRPSSPSPSVVSEKEK
                     EELERLQKEEEERKKRLQLYVFVMRCIAYPFNAKQPTDMARRQQKISKQQLQTVKDRF
                     QAFLNGETQIMADEAFMNAVQSYYEVFLKSDRVARMVQSGGCSANDSREVFKKHIEKR
                     VRSLPEIDGLSKETVLSSWMAKFDAIYRGEEDPRKQQARMTASAASELILSKEQLYEM
                     FQNILGIKKFEHQLLYNACQLDNPDEQAAQIRRELDGRLQMADQIARERKFPKFVSKE
                     MENMYIEELKSSVNLLMANLESMPVSKGGEFKLQKLKRSHNASIIDMGEESENQLSKS
                     DVVLSFSLEVVIMEVQGLKSLAPNRIVYCTMEVEGGEKLQTDQAEASKPTWGTQGDFS
                     TTHALPAVKVKLFTESTGVLALEDKELGRVILHPTPNSPKQSEWHKMTVSKNCPDQDL
                     KIKLAVRMDKPQNMKHSGYLWAIGKNVWKRWKKRFFVLVQVSQYTFAMCSYREKKAEP
                     QELLQLDGYTVDYTDPQPGLEGGRAFFNAVKEGDTVIFASDDEQDRILWVQAMYRATG
                     QSHKPVPPTQVQKLNAKGGNVPQLDAPISQFYADRAQKHGMDEFISSNPCNFDHASLF
                     EMVQRLTLDHRLNDSYSCLGWFSPGQVFVLDEYCARNGVRGCHRHLCYLRDLLERAEN
                     GAMIDPTLLHYSFAFCASHVHGNRPDGIGTVTVEEKERFEEIKERLRVLLENQITHFR
                     YCFPFGRPEGALKATLSLLERVLMKDIVTPVPQEEVKTVIRKCLEQAALVNYSRLSEY
                     AKIEENQKDAENVGRLITPAKKLEDTIRLAELVIEVLQQNEEHHAEPHVDKGEAFAWW
                     SDLMVEHAETFLSLFAVDMDAALEVQPPDTWDSFPLFQLLNDFLRTDYNLCNGKFHKH
                     LQDLFAPLVVRYVDLMESSIAQSIHRGFERESWEPVKSLTSNLPNVNLPNVNLPKVPN
                     LPVNIPLGIPQMPTFSAPSWMAAIYDADNGSGTSEDLFWKLDALQTFIRDLHWPEEEF
                     GKHLEQRLKLMASDMIESCVKRTRIAFEVKLQKTSRSTDFRVPQSICTMFNVMVDAKA
                     QSTKLCSMEMGQEHQYHSKIDELIEETVKEMITLLVAKFVTILEGVLAKLSRYDEGTL
                     FSSFLSFTVKAASKYVDVPKPGMDVADAYVTFVRHSQDVLRDKVNEEMYIERLFDQWY
                     NSSMNVICTWLTDRMDLQLHIYQLKTLIRMVKKTYRDFRLQGVLDSTLNSKTYETIRN
                     RLTVEEATASVSEGGGLQGISMKDSDEEDEEDD"
     exon            829..942
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1275
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1356
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1590
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1712
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1824
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1964
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2031
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2140
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2353
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2490
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2491..2678
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2679..2780
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2781..2844
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2845..2968
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2969..2986
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            2987..3093
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            3094..3114
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            3115..3271
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            3272..3413
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            3414..3560
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            3561..3716
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            3717..3864
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..3939
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            3940..4023
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            4024..4056
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            4057..4164
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            4165..4269
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     exon            4270..5507
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5481..5486
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /note="hexamer: AATAAA"
     polyA_site      5507
                     /gene="CADPS"
                     /gene_synonym="CADPS1; CAPS; CAPS1; UNC-31"
                     /note="major polyA site"
ORIGIN      
        1 aatcggcaag atgcctcttc ggctcttggc tgcatcgaca gcttgcaaca ctcggcatct
       61 tttctggagg cgcctccttc agcagccgca gatggcatcc ggctgcgggc tcggggctcg
      121 caattgattc tcccccttgc ccacctcgag tccacggacg cacctctccc ttcccctcct
      181 cccttcgcgc ttctgggtct gagcccagct cgcgaccgcc gggcagagga tcagtcgcgg
      241 cggccgaggc tgagcagcag cgctctcgct ccctgacctg gggagaagcg cccacccggg
      301 agagctgatc cccggctgcc tccagcgccc cccacctttt gcactccaag ccgggggctc
      361 cagagacccc gctccccagg cgccactatg ctggaccctt cgtccagcga agaagaatcg
      421 gatgagatcg tggaggagga gagcggcaag gaggtgctcg gctcggcccc gtccggcgcg
      481 cgcctgtctc ccagccgtac cagcgagggc tcggccggca gcgccgggct ggggggcggc
      541 ggcgccggcg ccggagccgg ggtgggtgca ggcggcggcg ggggcagcgg cgcgagcagc
      601 ggcggcgggg ccggggggct gcaacccagc agccgcgctg gcggcggccg gccctccagc
      661 cccagcccgt cggtggtgag cgagaaggag aaggaagagt tggagcggct gcagaaagag
      721 gaggaggaga ggaagaagag gctgcagctg tatgtgttcg tgatgcgctg catcgcctac
      781 ccctttaatg ccaagcagcc caccgacatg gctcgccggc agcagaagat cagcaaacag
      841 cagctgcaga cagtcaagga ccggtttcag gctttcctca atggggaaac ccagatcatg
      901 gctgacgaag ccttcatgaa cgctgtgcag agttactatg aggtgttcct gaagagcgac
      961 cgtgtggccc gcatggttca gagtggaggc tgttccgcca acgactcccg ggaggtcttc
     1021 aagaagcaca ttgagaagag agtgcgcagc ctgcctgaga ttgacggcct cagcaaggag
     1081 actgtgctga gctcctggat ggccaaattt gatgccatct accgtggaga agaggacccg
     1141 cggaagcagc aggcccggat gacagccagc gcagcctccg agctgattct gagcaaggag
     1201 caactctatg agatgttcca gaacattctt gggatcaaga agttcgaaca tcagctcctt
     1261 tacaatgcct gccagctgga caatccagat gagcaagcag cccagatcag acgagagctg
     1321 gatggacgtc tacaaatggc agaccaaata gccagggaac gcaaatttcc caagtttgta
     1381 tccaaagaaa tggaaaacat gtacattgag gagctgaagt catctgtcaa cctgctcatg
     1441 gccaacttgg agagcatgcc ggtatccaaa ggcggggagt tcaagctcca gaaactcaaa
     1501 cgcagccaca atgcttccat catcgacatg ggcgaggaga gtgagaacca gctctccaag
     1561 tcagatgtcg tgctgtcttt ctcattggag gtggtaatta tggaagtcca aggcctcaaa
     1621 tctttggctc caaatcgcat cgtatattgc acaatggagg tggaaggagg agagaaacta
     1681 cagactgatc aggccgaggc ttctaaacca acctggggca cccagggtga cttctccaca
     1741 acccatgcac tgccagctgt gaaggtgaag ctgttcacag agagcacagg cgtcctggcg
     1801 ttggaggaca aggagcttgg gcgggttatt ctccatccca ccccgaacag ccccaaacag
     1861 tcagagtggc acaaaatgac agtctccaaa aactgccccg accaagatct caaaatcaaa
     1921 cttgctgtcc gaatggataa gcctcaaaac atgaagcatt ctgggtattt atgggccatc
     1981 ggtaagaatg tctggaagag atggaagaaa aggttttttg tattggtgca ggtcagtcag
     2041 tacacgtttg ccatgtgcag ttatcgggag aagaaagcgg agcctcagga acttctacaa
     2101 ttggatggct acactgtgga ttacaccgac ccccagccag gtttggaggg tggccgagcc
     2161 ttcttcaatg ctgtcaagga gggagacacc gtgatatttg ccagtgacga tgaacaagac
     2221 cgcatcctgt gggtccaggc catgtatcgg gccacggggc agtcacacaa gcctgtgccc
     2281 ccgacccaag tccagaaact caacgccaag ggaggaaatg tacctcagct ggatgcccct
     2341 atctctcaat tttacgcaga tagagctcaa aaacatggca tggatgaatt tatctcttcc
     2401 aacccctgta actttgacca cgcttccctc tttgagatgg tacaacgcct tactttggat
     2461 cacagactta atgattccta ttcttgcctg ggctggttca gtcctggcca ggtgtttgta
     2521 ctagacgagt attgcgcccg aaatggagtc cgggggtgtc accgacatct ctgctacctc
     2581 agagacttgc ttgaacgggc agaaaatggc gccatgatcg accccaccct tcttcactac
     2641 agctttgcct tctgtgcatc ccatgtccat gggaacaggc ctgatggaat tggaactgtg
     2701 actgttgaag aaaaggaacg ttttgaagaa atcaaagaga ggctccgagt tctgctagaa
     2761 aatcagatta cacattttag gtattgcttt ccatttggtc gacctgaagg tgctttgaaa
     2821 gctactctct cactcttgga aagggttttg atgaaagata ttgttacccc agtgccacaa
     2881 gaggaggtaa aaacagttat ccgtaaatgt ctggaacagg ctgcgttagt caactattct
     2941 cggctctcag agtatgccaa aatcgaagag aatcaaaagg atgcagaaaa tgtaggccgg
     3001 ttaatcactc ctgccaaaaa gcttgaagat acaatacgtc ttgctgaact agtcattgaa
     3061 gttcttcagc aaaatgagga gcaccacgca gagccacatg ttgataaagg agaagccttt
     3121 gcgtggtggt cagatttaat ggtggagcat gcggagacgt tcctgtcact ctttgcagta
     3181 gacatggatg cagccttaga ggtgcaacct ccagacacat gggacagttt tccactattt
     3241 cagctgctga atgattttct ccgtactgac tataatttgt gcaatggaaa atttcacaaa
     3301 cacctgcaag acctgtttgc cccacttgtt gttagatatg tggatctgat ggagtcctca
     3361 attgcacaat ccattcacag gggctttgag cgggagtcat gggaaccagt caagagttta
     3421 accagtaacc tacccaatgt gaacctaccc aatgtgaacc ttcccaaagt accaaatcta
     3481 ccagttaaca tccctctagg catcccacaa atgcctactt tttcggcacc gtcatggatg
     3541 gctgctatat atgatgcgga taatgggtca ggcacctcag aagatctgtt ttggaaactt
     3601 gacgcccttc agaccttcat tcgggacctg cactggcctg aagaagagtt tggaaagcac
     3661 ctggaacaac ggctgaagtt gatggcaagt gacatgatcg aatcttgtgt caaaagaacc
     3721 aggattgcat ttgaagttaa gctgcaaaaa accagtcgat caacagattt tcgagtccca
     3781 cagtcaatat gcaccatgtt taatgttatg gttgatgcca aagctcaatc aacaaaactt
     3841 tgcagcatgg aaatgggcca agagcatcaa taccattcaa aaatagacga actaattgaa
     3901 gaaactgtta aagaaatgat aacactcttg gttgcaaagt tcgttactat cttggaagga
     3961 gtgctggcaa aattatccag atatgacgaa gggactttgt tttcttcttt tctgtcattt
     4021 accgtgaagg cagcttccaa atatgtggat gtacctaaac ccgggatgga cgtggccgac
     4081 gcctacgtga ctttcgtccg ccattctcag gatgtcctgc gtgataaggt caatgaggag
     4141 atgtacatag aaaggttatt tgatcaatgg tacaacagct ccatgaacgt gatctgcacc
     4201 tggttgacgg accggatgga cttacagctt catatttatc agttgaaaac actaattagg
     4261 atggtaaaga aaacctacag agatttccga ttgcaagggg tcctggactc caccttaaac
     4321 agcaagacct atgaaacgat ccggaaccgt ctcactgtgg aggaagccac agcatcagtg
     4381 agtgaaggtg ggggactgca gggcatcagc atgaaggaca gcgatgagga agacgaagaa
     4441 gacgattaga ccatttggtc ctagagtctg ctgggacaga gtcctgtaat cagtgcatgt
     4501 ccttagtctg ttagttaaac ccattaggaa ttttctgtca actaccatgc ccatgagatg
     4561 tttatcaata caactgccat tttagctatg tggtaccaag attagcaaat gaccttcata
     4621 tccactgatt tcctgatgtc catgtctata tgtttacaag caatatggag caccattctt
     4681 taaatactgt tcatggagaa tacatagtct aaccactagg cgtgtccctg ttatcagcaa
     4741 agatcaatga tgcttcattc atgtactatg tatgcattgg tggtaaatgg atgtgagggc
     4801 aagtacatca agtacattca ctctgtttca cgtatgtgga tgccagttaa ttaaatgagt
     4861 acgtaaataa attaattaaa acacatagat ctgctttgtg tttttatttt tattttttga
     4921 aaaacaaaag gcaagtctcc aacaattaac ttttgatgct ttctgttccc ctaaaaccaa
     4981 aaaatgaacc ccttgtgtcg ttgttaaccc atcctttcat ttactcatat aattagccaa
     5041 aaaaaaaagg atggctacat accaatggat tgattctctt aattgccacg gcaagggggc
     5101 gatcctatca tgacttaaca tcaagcgcgc agttcaaaac tactgtcttc tgtcaaagtt
     5161 ttctcctctt aaatgttatt ttgcttttac gtctcaactg tgtatgtaaa aaaaatgaat
     5221 atttaaatta caaccctaga ctaaaaatgt gtttataata agatgtggat atttccttca
     5281 gtagattgta accataattt aaattatttt gttccacact gttttttata tctgtcatgt
     5341 acattgcatt ttgatctgta actgcacaac cctggggttt gctgcagagc tatttctttc
     5401 catgtaaagt agtggatcca tcttgctttt gccttatata aagcctacag ttatggaagt
     5461 gtggaaaact gtggcttctc aataaatatt cagatgtcct aagaata
//
