LOCUS       NM_153810              11035 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens CDK2 associated cullin domain 1 (CACUL1), mRNA.
ACCESSION   NM_153810
VERSION     NM_153810.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Rashpa,R., Klages,N., Schvartz,D., Pasquarello,C. and Brochet,M.
  TITLE     The Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator required
            for the formation of gametes and motile forms in Plasmodium berghei
  JOURNAL   Nat Commun 14 (1), 1312 (2023)
   PUBMED   36898988
  REMARK    GeneRIF: The Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator
            required for the formation of gametes and motile forms in
            Plasmodium berghei.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11035)
  AUTHORS   Zhang,X., Zhang,T., Han,X., Qiu,Z., Cheng,J., Gao,X. and Gou,X.
  TITLE     CDK-associated Cullin 1 Promotes Cell Proliferation and Inhibits
            Cell Apoptosis in Human Glioblastoma
  JOURNAL   Curr Cancer Drug Targets 21 (10), 870-880 (2021)
   PUBMED   34080964
  REMARK    GeneRIF: CDK-associated Cullin 1 Promotes Cell Proliferation and
            Inhibits Cell Apoptosis in Human Glioblastoma.
REFERENCE   3  (bases 1 to 11035)
  AUTHORS   Chen,N., Kong,Y., Wu,Y., Gao,Q., Fu,J., Sun,X. and Geng,Q.
  TITLE     CAC1 knockdown reverses drug resistance through the downregulation
            of P-gp and MRP-1 expression in colorectal cancer
  JOURNAL   PLoS One 14 (9), e0222035 (2019)
   PUBMED   31504073
  REMARK    GeneRIF: CAC1 plays an important role in the occurrence and
            promotion of antineoplastic drug resistance in colorectal cancer.
            CAC1 knockdown resulted in decreased P-glycoprotein and MRP-1
            protein expression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11035)
  AUTHORS   Hu,Y., Lao,L., Mao,J., Jin,W., Luo,H., Charpentier,T., Qi,S.,
            Peng,J., Hu,B., Marcinkiewicz,M.M., Lamarre,A. and Wu,J.
  TITLE     Armc5 deletion causes developmental defects and compromises T-cell
            immune responses
  JOURNAL   Nat Commun 8, 13834 (2017)
   PUBMED   28169274
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11035)
  AUTHORS   Fukuda,T., Kigoshi-Tansho,Y., Naganuma,T., Kazaana,A., Okajima,T.,
            Tsuruta,F. and Chiba,T.
  TITLE     CACUL1/CAC1 attenuates p53 activity through PML post-translational
            modification
  JOURNAL   Biochem Biophys Res Commun 482 (4), 863-869 (2017)
   PUBMED   27889610
  REMARK    GeneRIF: These data suggest that CACUL1 is a novel regulator that
            negatively controls p53 activity through the regulation of PML
            SUMOylation.
REFERENCE   6  (bases 1 to 11035)
  AUTHORS   Kong,Y., Bai,P.S., Sun,H., Nan,K.J., Chen,N.Z. and Qi,X.G.
  TITLE     The deoxycholic acid targets miRNA-dependent CAC1 gene expression
            in multidrug resistance of human colorectal cancer
  JOURNAL   Int J Biochem Cell Biol 44 (12), 2321-2332 (2012)
   PUBMED   22903020
  REMARK    GeneRIF: miR-199a-5p can regulate CAC1 and function as a tumor
            suppressor in colorectal cancer.
REFERENCE   7  (bases 1 to 11035)
  AUTHORS   Moon,M., Um,S.J. and Kim,E.J.
  TITLE     CAC1 negatively regulates RARalpha activity through cooperation
            with HDAC
  JOURNAL   Biochem Biophys Res Commun 427 (1), 41-46 (2012)
   PUBMED   22982681
  REMARK    GeneRIF: CAC1 is a novel corepressor of RARalpha that cooperates
            with HDACs and is involved in the regulation of RA-induced cellular
            differentiation.
REFERENCE   8  (bases 1 to 11035)
  AUTHORS   Kong,Y., Bai,P.S., Sun,H. and Nan,K.J.
  TITLE     Expression of the newly identified gene CAC1 in the hippocampus of
            Alzheimer's disease patients
  JOURNAL   J Mol Neurosci 47 (2), 207-218 (2012)
   PUBMED   22415352
  REMARK    GeneRIF: CAC1 is an important factor for the protection of cells
            against amyloid beta toxicity and oxidative stress in Alzheimer's
            disease.
REFERENCE   9  (bases 1 to 11035)
  AUTHORS   Kong,Y., Nan,K. and Yin,Y.
  TITLE     Identification and characterization of CAC1 as a novel
            CDK2-associated cullin
  JOURNAL   Cell Cycle 8 (21), 3552-3561 (2009)
   PUBMED   19829063
  REMARK    GeneRIF: CAC1 is a novel cell cycle associated protein capable of
            promoting cell proliferation.
REFERENCE   10 (bases 1 to 11035)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AJ544771.1, AY743663.1, AL139407.11 and BU623607.1.
            
            On Nov 22, 2018 this sequence version replaced NM_153810.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.18020.1, AY743663.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369151.8/ ENSP00000358147.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1341              AJ544771.1         61-1401
            1342-3347           AY743663.1         1619-3624
            3348-5814           AL139407.11        11165-13631         c
            5815-6464           BU623607.1         19-668              c
            6465-11035          AL139407.11        5944-10514          c
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.11"
     gene            1..11035
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /note="CDK2 associated cullin domain 1"
                     /db_xref="GeneID:143384"
                     /db_xref="HGNC:HGNC:23727"
                     /db_xref="MIM:618764"
     exon            1..575
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..1318
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /note="cullin; Cdk-Associated Cullin1; cdk-associated
                     cullin 1"
                     /codon_start=1
                     /product="CDK2-associated and cullin domain-containing
                     protein 1"
                     /protein_id="NP_722517.3"
                     /db_xref="CCDS:CCDS41570.1"
                     /db_xref="GeneID:143384"
                     /db_xref="HGNC:HGNC:23727"
                     /db_xref="MIM:618764"
                     /translation="MEESMEEEEGGSYEAMMDDQNHNNWEAAVDGFRQPLPPPPPPSS
                     IPAPAREPPGGQLLAVPAVSVDRKGPKEGLPMGPQPPPEANGVIMMLKSCDAAAAVAK
                     AAPAPTASSTININTSTSKFLMNVITIEDYKSTYWPKLDGAIDQLLTQSPGDYIPISY
                     EQIYSCVYKCVCQQHSEQMYSDLIKKITNHLERVSKELQASPPDLYIERFNIALGQYM
                     GALQSIVPLFIYMNKFYIETKLNRDLKDDLIKLFTEHVAEKHIYSLMPLLLEAQSTPF
                     QVTPSTMANIVKGLYTLRPEWVQMAPTLFSKFIPNILPPAVESELSEYAAQDQKFQRE
                     LIQNGFTRGDQSRKRAGDELAYNSSSACASSRGYR"
     exon            576..702
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            703..805
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            806..901
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1004
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1094
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1233
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1277
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..11035
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5919..5924
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /note="hexamer: AATAAA"
     polyA_site      5943
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /note="major polyA site"
     regulatory      6438..6443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /note="hexamer: AATAAA"
     polyA_site      6458
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
     regulatory      11009..11014
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
                     /note="hexamer: AATAAA"
     polyA_site      11035
                     /gene="CACUL1"
                     /gene_synonym="C10orf46; CAC1"
ORIGIN      
        1 agagccccgg agcgcccacc gccgtcagcc gcgtcttcaa gctagtcggc cccgcggaga
       61 gcgagagacc ctgcgcaccg cagccctctc cgctgcgccc attcctgccg ccggcgatgt
      121 aactcgggga ggcagcggtg cccgctggcc cggggttgag acaggcggca ggtgcctgtg
      181 aggcgggcgg gtgccggggg ccagcaggat ggaggaaagc atggaagagg aggagggggg
      241 cagctacgag gcgatgatgg acgaccagaa ccacaacaac tgggaggctg cggtggacgg
      301 cttccggcag cccctgccac ctccgccgcc cccctcgtcg atcccggccc ctgcccgaga
      361 gcctccgggg gggcagctgc tggcggtgcc cgcggtctcc gtggacagga aaggccccaa
      421 ggaggggctc ccgatggggc cgcagccacc gccggaggct aatggggtga tcatgatgtt
      481 gaagagctgc gacgcggccg ccgccgtggc caaggcggcc cccgccccca ccgccagctc
      541 caccatcaac atcaacacct ccacctccaa gttcttaatg aatgttataa ctattgaaga
      601 ttataagagc acatactggc caaaattgga tggtgccata gatcaacttt taactcagag
      661 tcctggtgac tatatcccca tatcctatga acagatatac agttgtgtgt ataaatgtgt
      721 atgccagcag cactcggaac agatgtatag tgatctgatt aaaaagataa ctaatcactt
      781 agagagagtc tcaaaggagc tgcaggccag ccctccagat ctctatattg aaagatttaa
      841 tatagctctt ggacaatata tgggagcatt gcagagcatt gtgcctcttt tcatatatat
      901 gaataagttt tacatcgaaa ccaagcttaa cagagactta aaagatgacc ttataaagct
      961 gtttacggaa catgttgcag aaaagcacat ttacagccta atgcctttac ttttagaagc
     1021 ccagtcaaca ccatttcagg tcacaccttc aactatggca aatattgtga aaggcctgta
     1081 taccctcaga ccagagtggg ttcagatggc tccaactcta ttttctaaat ttattccaaa
     1141 cattctccct ccggcggtgg aatctgaact ttctgaatat gctgctcaag atcagaaatt
     1201 tcaaagagaa cttatacaga atggttttac aaggggtgac cagtcccgga agagagctgg
     1261 ggatgagttg gcttataata gctcgtcagc atgtgcaagt tccagggggt acagatagtg
     1321 aatgtattga atatgctttc cttcctgaaa agaggacaca ctggagctgc agagactgta
     1381 ttcagagcac agtgggggct gcacacactc aggagctctg tcacaaagct gttcatggaa
     1441 gaggatgttg gacttcttac ttggtttgta attttaaaac aaaaactaaa aaaaaaaaaa
     1501 acgttgctgc tagacttggg ggtgattttg aaatgggaca atcttttaat gagtttatct
     1561 acagattctg tgaggaaaaa gcatctcaga aagtgaccat ttctaagtga aatctgacag
     1621 ccaaaatcag cagcttcctc caaaaatata agagcagaag aatttttttt ttaagcactt
     1681 gttttgttca tttgtggact tggcattttg gtgtattggt ttcacatgtc aggatctctt
     1741 ttgctttaaa accaagcaga tcattacagt acagtatttt tcacccacaa ccaaaacaac
     1801 cccttttaaa agagttggtc tttgtttagc attagaaagt cttgttaaag gaaaatgcta
     1861 actctgcttt tgctagaact ttcccccatc agttcacaac tttcttctac tctgtgcctt
     1921 gagctttgtg cactttgcaa ctgtgtgacc atgttgtcaa actcctcctg gagaagtgta
     1981 tggtatctaa actcacccac caagatggca ggtgtggccc tgtagcagga tgctaattaa
     2041 gtgggaaaat agaaaccttt tgcacatggg tcggattttc ccctggttca ccagagcata
     2101 ttcatattaa tgttggggac caactctcct agagcatttc cattccctgt gccacttaac
     2161 acgatgtctg tcaccagctg ctatagtatt ggcagtactc tagtttaggg atgggcggta
     2221 ctttagcacg gaagcttccc ttagtattat tgagagctac ttcctgcgat gctctaggaa
     2281 gcagatacag ttattaagtc caaatacaca gtggtgtggg gttcctgatc acttcatttt
     2341 aaatgaatga gataattact taaaaaaagt ctgtcatttg aacattatat ttgagtgtga
     2401 tagggaagaa gtagcactgc tgtcctaagg ctcccttcca gctgtggcaa agctatattt
     2461 ctgtttctcc acatccacag tgctttttag tccatcataa aagtttctga agcttattct
     2521 agctgttgtt gaaagcccaa gaaaaataga tgtggcaaga gaaaaatact tttcttctca
     2581 attttgcttg aatcttaatt gagcataggc ttctctacta ggcaacatca gactaatagt
     2641 atttacctct tcagagaact tgaggggaaa agaaacatcc cagggatgct gtcgcatgtc
     2701 tgctctgcag gttcttcata cagctgcctc tgccaattca gtttattttt tggggggagt
     2761 ggtgtgggtg ggagagttag aagtgggata ttgggagcaa gtaagtcacc caataattga
     2821 agtccttgat ttgtaaaact gttatacaga gaagtaaaga taaaggttgt cagatttatt
     2881 ttttaaagta cttaaccaga gttcgtggat tttgtggact ttaattggac tcaattcact
     2941 ctgtgagcat cctggattgc atcgatgaac actgaattac tgtaagaaca tagacaggct
     3001 ctcactatga cctggacagt ttaagttgct acactggtaa cattctagta tcaaggagaa
     3061 ttccattcat catgacattc acattttact tttctcaatg gaatgcttgc ttcaggctta
     3121 ccaatcacac ctagaactga agtctgagtg agtggtatta ataatttttc tgaaaggaat
     3181 aaaattggaa tgtttctaaa tgtggagaaa ttcttcagta tcctaataat atacccaaat
     3241 gctttttctg ggagagagca ttcttttaca aaagaacttt ccctataaaa gcatcacagt
     3301 ataatgtgaa ttggcattaa aatctttgga tgcttttgca ttattaagtt catggagaaa
     3361 tatattcttt ttactatgta tgtattaata aaaagtgggc aaagtaaagt ttgcaaaatt
     3421 tagtttctct aaatttttgc acagtgactg cagctggcta tgggtcagct cttaatacaa
     3481 caaaaccttg actggaagtc actatagaaa ggttgtacat agtctcccag tctacatgtc
     3541 ctggctgtta atcatcttgg ccccttgagg cacatcacag tttgaaggac ctgtttaagt
     3601 tgaaatagac tttgcttatt tattgggatt ctaaaaaatt ctgagtgagt ttgcagtatg
     3661 agaggaaata agatttcctc ctccttcctc tcattttata ttgactgttt gccagaaact
     3721 gttttcttct gttttcttat attttgtttt tgagatggag tctcactctc tcacccaggc
     3781 tggagtgcag tggtgcaatc tcagctcact gcaacctctg cctcctgggt tcaagtgatt
     3841 ctcctgcctc ggcctcctga gtagctggaa ttacaggcac gtgccactac gcccggctac
     3901 tttttgtatt ttgtttttag tagagacggg gtttcaccat gttggtcagg ctggtcttga
     3961 actcctgacc tcaagtgatc cacccacctc ggcctcccaa agtgctggga ttataggtgt
     4021 gagccactgc acctggcctt tttttttttt ttttttgtat tcttgccagt acagtatatg
     4081 gtttttctac cccaattaca tactgggttt tgtaccacat cactaaaggc ccaaatcatt
     4141 gaagatacaa aaccgtacat gcaggctggt tgtctggtta gtcaatggct gatttgcttc
     4201 aactgtctag tatgtatgtg cagcctgaaa ctggctcctt aaaaggaaag ccgggtcagt
     4261 catcttgaaa aaatgacatg taaaagtaaa tcgataattg ttttgagaga cggtacatgt
     4321 tttaaaggtt ggccttaagc ttcagtaaca ttgtcatttt gtgacctttt gttgtcacac
     4381 ctgtacccta acctgacagg aattaactac tgtttttttg tggggcagaa agcaaaacct
     4441 ggtgttgtga cttttatcct aatggttctt aggcaaggtt agtgagaaga aacacaaacc
     4501 cagatgcatg cattgtgcat tattttgtag acaagctact ttttcttctg tccctttaac
     4561 aaatttgcag caattaccct ccctttgggg tctagagtga aagctaattt gtgggtagat
     4621 gagattgcag aagaatggat gtccatggct gtgaacactg cacactgcac atccatctcc
     4681 agtgctcaca ctgtgcagct accactccct ggctgcgtgc catgctgtcg ggttgcagat
     4741 ttgcacacat aaattcctca ggaagagttt gcatgagcat cacctcgcaa tattctgtac
     4801 tgaccaaaca agggatttga acgtttttca gcacaaaagg ataacttccg agtggtggtc
     4861 tgtacgcata ctagcaaagg taatggtgat ctagcaaaca aaattggttt ctgcagttag
     4921 aagtgagcag gagcacttgt attatagtat ttaaataatc ctggttaatc tctttttaag
     4981 ccgagtaacc cctccagatt ttgccttttt attattgagg ctggctttat tttcttctac
     5041 tttttttccc gttttatagc agttaattat ttttgtgatt attatgcaag aagcattgcc
     5101 cttgagttaa actgttattg tttcataagc agctattaaa ataactgagc attgttttat
     5161 gaacatacac taatctgaga tactgaaaag ctttgcaact aaaaagcaaa acaacctaca
     5221 ttagtcatct agccattgtt tggatgtttt gagttgattt tttatggtgc ctcttttagc
     5281 ttggaatatt acgtttactt taatccaagt ctaggccttt taaagggtcc ttaaaattaa
     5341 agttcagaat gtgaatccct ttgacatcta ttacaggttt ataggacctt tttggttgtg
     5401 attactgttt tcaatacgat tgtataaatg aagttaactt tgtcagaagt taaaatggag
     5461 gtcataggag ttcctggaga aatggctctc ctgtttcttt cattacccca ctgaagttca
     5521 ccccagtttc tggccacaag aatatgagaa aggaaccctg ttgttttcca agggaaatca
     5581 ttcctctctg tccccactgt tgattaacta aagtcctgga caccttcctt cctccactgg
     5641 ccaagaccca ccttgaccca ccttgaacct cttttcagag ccgagtggca tgaatatgtg
     5701 tactgtttct gcttctgttg atggagtggc tgtgggagaa ttaaaggaaa tgctaatttg
     5761 agcttcattc ataggggaac ctactatata ttgcatccct gctggttgga aattatcttc
     5821 atctctggac tgcattgttt agaaaaatgt taatggctta caattctgag aactttattg
     5881 tgtggctctg gggttaagaa ttctgtggtt tgaaaaaaaa taaatatttt gtattgattc
     5941 tcacgtcatt tcaatgttgt gactatgtac taaatgcact aagactgggt attctcttag
     6001 aagagtgcgt tttgttaaac agatggcagt tcactctcat tagttctatt tgtcaatatt
     6061 acagccacac acttcaggat aacttactca aatgtgaagt catgggaagc tatggctagt
     6121 aacaggaatg cttatgaaaa atttggaggg caagcacgag caagaggctt ttgccactca
     6181 ctagcacagc cagagcaagg atgaagcaat gaagtcttgt tcctagtggt gcttgtatgt
     6241 cagaagccat agtgagctca gcacagggcg ttctgcacat catctcttta atccaagaaa
     6301 aacaaaatgg cggatgaaac tcctattgac ctgggataca aagccactga ctttggctct
     6361 tgatcttttg gtgggttctg aatcactcct tctgtattag ggcatatact ttcactctaa
     6421 aacattgtgg taaatgaaat aaatcttgta cagatgtcgt tggccttgtt atgttatgtt
     6481 ataaatcctg tacagatgtc cttggcctta atacctggtt cactggaact ttgttataat
     6541 atagaatatt tgattttgct tatagcatat taatattgcc tcctacatga cagccacact
     6601 gagcacacat ctgttaccac agttacttag cctgcagggt gagtgttgat cttgacttct
     6661 gcaaccagtg ctaaaaaaca gtgtgtaaca atatacatat ggtcatatct attctatatt
     6721 catatatagt atggatacgg atatcagtat atttgtagtt tttgtttttt aaacactgta
     6781 cagtttcttc tgtggacctt taatcaactt ttggacttag gttgtttttt tctttgcatt
     6841 tattggtact atgctttttt ataacccttt gtgttttctt gctatgaaat tagtgtcatc
     6901 ccctaggtta tttgtaacct tactttaagg ttttagtttc tcttgctatc tctgtagagg
     6961 tgtcctttcc tacagtattg tcagaatgcc agttctcatc ccttccattt ttttgacctc
     7021 agctctgttg tgatgttatc tactttttgg ttatcacatg tggcagaatt cagtgactct
     7081 gacaatttcc attttcactc ccctgcccca cagttaagta gggtgtcatt taaactcttt
     7141 cccttttagc aggtttgttt tttactaagt acaaaccttt tctactagaa ccagctctgt
     7201 gtgtgtgtgt gtatatgtgt gaattttatt tcttcgattg gcctgtttct caattatcta
     7261 aaattctaaa ataccttgaa tatcaaaaag catgatagta gaatgaatat cacacatatt
     7321 aatagaacct gagagcaact agcttaacta gctgtagtct tgagtaggtt ttttttttaa
     7381 tctgttttcc tttattaagc cctctccctc cctaaattgt taagccagaa gggcataaca
     7441 attcaaaact ttctagtaat gtacctacat gtctaatagt atacctcaaa atatgtaaag
     7501 aaaaagtggc tcacacctgt aatcccaaca ctgagaggct gaggtgggaa gattgcttga
     7561 agccaggatt tgagaccagc ctgggtaaca cagaacccca tctctacaaa aaaatttttt
     7621 aaattagggc cgagcatggt ggctcacgcc tgtaatccca gccctttggg aggctgaggt
     7681 gggcgtatca tttgaggtca ggagttcaag accagcctgg ccaacatggt gaaattccat
     7741 ctctactgaa aatacaaaaa ttagctgggc gtgggggtac acgactgtaa tcccagctac
     7801 tcgggaggct gaggcaggaa aatcgcctga acccaggagg cagaggtttc agtgaaccaa
     7861 gattgcacta ttgtactcca gcctgggcga caagagtgaa actatctcaa gcaaaaaaca
     7921 aaaaaagttg ggcctggcac agtggctctt gcctgtaatg tcaatacttt gggaggctga
     7981 ggcaggtgga tcacttgagg ccaggagctc gagaccagcc tggctaacat ggtgaaaccc
     8041 tgtctctaca aaaaataaaa aataaaaata aattaaaaaa aaattaggca tggtggcata
     8101 cacctgtaat cccagctgct tgggtggctc aggcacaaga acttgaaccc aggaagtgga
     8161 ggttacagtg ggctaaggtc acaccactgg actctggcct gagcaacaga gcgagactct
     8221 gtctcaaaaa aacacaaaaa aagttgggcg tggtggtacc tgcttttagt cctagttatt
     8281 tggaggctaa ggtgtgaggc aggaggattg cttgagccca ggagtttgag gctgcattga
     8341 gctatgatga taccactgca tttcagctag gataacagag tgagaccctg tcgagaaaaa
     8401 tatttaaaaa gttaaaagaa aactacaaaa aggaaaaatg aacacactac aaagagaaat
     8461 aggtcagtct accaacataa tgggcatttt caacataccc ctctcagtaa ttaatagatc
     8521 ttgcagagac taaattatga agattattaa ggacatagaa gatttaaata gtacagctaa
     8581 tcaatttgat ctgacagata cataacacac acatacataa aacacattgt acccttcagt
     8641 aggaaaataa gtttttgcaa accaagtcta acagtgtatt caaaatgtaa caaatgatga
     8701 ccaaaatgga ttggtctcag gaatacaaga ttggcttttt aatgtaatta agaaatgctg
     8761 tttataatag gacaagaaaa agtacctaag aataaatttt aaaatgagaa aatgataaaa
     8821 aaaaaactta atggacctaa ataagtgtag agaatataag ggataacact caatattatc
     8881 taaatctcaa ctttttctcc aaattgcaat tccaaaaaaa ttcccaatga ttttcactgt
     8941 ggaacctgac aatttgattc gtcgatataa atggaagagc aaagggccaa ggatatccaa
     9001 gacctttctg aatggattgt gaggggaact agcactacaa gattctaaca cttactgcca
     9061 aaggtagtga ttaaaacaat gactttggag tagggaagaa tttcatcaac aagatagaaa
     9121 atgcagacta tcagtagata agtaatttgg ctaccattga ttgtaagtac ttattaccaa
     9181 acatgctgta gagtgaaaag agaagccaaa tctggaataa gatacaaata ttcataatat
     9241 aactgctaaa gagcacctga atgcaaatca gcaatgaaaa caactcagga gagacggacc
     9301 ccaaacacta gcaggcattt cacagaagaa acagaaatgg cccataaatg tgaaaagatg
     9361 ttcagcctca ttagtcgtca ggtaatgcac attaaaagca tagataccat ttccttgctg
     9421 acaacttggc agaaattatg agaagtctga aaataacaat ctgagagttt atgacaacaa
     9481 accttcacct gttgttggcg tgtaaatctg cacttctact ttggagaaca gtattatcta
     9541 atgaaattga gcataggcac ctcttaatag cccactattt atacttagaa aaactcatgt
     9601 gcatgtgtac caagaaacgt atacagggat gtttataaca gcattatgtg taataattaa
     9661 aaggaatgaa ggagggagcc aaatgcccat ctagcataca gtgaataaat gaagtgtatg
     9721 ctgtggaatt ctctagagtg gtcaaggcat cataccactg cgggcttcaa catggatgca
     9781 tctcagaaat cattgagtga agtcagaata gagccagcat gggtaccttt atgtaaagtt
     9841 caaaatcaga aaaaactaag tatattgttt agggactcag acacaagtag taaagctata
     9901 aaggaaagac agttattaac attcaggaac atagtttcct caggagaggg tggtggaggg
     9961 gaatgagaac agaggggaga acggggactt ggaagcactt cataatatga acgtttggac
    10021 cattctaaac atgccagttc tctgagttaa tgtatattca gtgtaaagcc aattcagata
    10081 accaacagta tttatataat ctaaaaaact catggaagta taaacagatg aaaataggaa
    10141 aatccccatt gaaagtgaag aaaatggaag gaacttgctg taccaaaaaa aatcactttc
    10201 taagtgcaat taaattaaaa gacttatgcc agggtaaaca gataagtcaa tggaaggtaa
    10261 tttagaaaat agaaaactca aatttagaat ttagagtaca ctaaaggcgg ctttttgaaa
    10321 tcatgctgtc catggtcaat tattttatga aaggtgctgg tataattaga gaacattaaa
    10381 ctgattccta tcataccatt caccaagaga aattccagat tgtgaactaa aaaagaaaaa
    10441 aatgttgtaa aaactagaaa gtagtatttt ttcttgggat tggagaagtt ttttctaaga
    10501 atacaagcaa tgaaaccgaa aaccctatta aactcactca cctgccttta atgccgtagt
    10561 gtgcacaccc cagtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtacat agacatatat
    10621 attttaaaat gtttaaattt gaacatatgt taccaataca tataaagcaa aagtagaaaa
    10681 tcaaaaagat gaagtaaaaa tgttttaaaa tattgtttgt ggtatttgtg gttaataaac
    10741 ctttgctctt tctaaaaaaa tgttctttta gagagagtca tgattaaaaa tgcttgtcat
    10801 ttttgaaact cctagtttta acactttata aaaacaactt aatgttctta aggtaagttt
    10861 attttcatgc ttggttttaa tgactattgt agctgaataa gtcacttcat taattaagat
    10921 ctgtggatcc aaatatggct gtgcttactg aaaatatttt tgagttccat ccaccaattt
    10981 tgcaaaagtg tgttgttgaa atttttataa taaattttca tcttttccaa tgtca
//
