LOCUS       NM_000726               7944 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens calcium voltage-gated channel auxiliary subunit beta 4
            (CACNB4), transcript variant 2, mRNA.
ACCESSION   NM_000726
VERSION     NM_000726.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   Coste de Bagneaux,P., von Elsner,L., Bierhals,T., Campiglio,M.,
            Johannsen,J., Obermair,G.J., Hempel,M., Flucher,B.E. and Kutsche,K.
  TITLE     A homozygous missense variant in CACNB4 encoding the auxiliary
            calcium channel beta4 subunit causes a severe neurodevelopmental
            disorder and impairs channel and non-channel functions
  JOURNAL   PLoS Genet 16 (3), e1008625 (2020)
   PUBMED   32176688
  REMARK    GeneRIF: The homozygous CACNB4 p.(Leu126Pro) variant underlies the
            severe neurological phenotype in the two siblings.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7944)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   4  (bases 1 to 7944)
  AUTHORS   Vendel,A.C., Terry,M.D., Striegel,A.R., Iverson,N.M.,
            Leuranguer,V., Rithner,C.D., Lyons,B.A., Pickard,G.E., Tobet,S.A.
            and Horne,W.A.
  TITLE     Alternative splicing of the voltage-gated Ca2+ channel beta4
            subunit creates a uniquely folded N-terminal protein binding domain
            with cell-specific expression in the cerebellar cortex
  JOURNAL   J Neurosci 26 (10), 2635-2644 (2006)
   PUBMED   16525042
REFERENCE   5  (bases 1 to 7944)
  AUTHORS   Helton,T.D. and Horne,W.A.
  TITLE     Alternative splicing of the beta 4 subunit has alpha1 subunit
            subtype-specific effects on Ca2+ channel gating
  JOURNAL   J Neurosci 22 (5), 1573-1582 (2002)
   PUBMED   11880487
REFERENCE   6  (bases 1 to 7944)
  AUTHORS   Escayg,A., Jones,J.M., Kearney,J.A., Hitchcock,P.F. and
            Meisler,M.H.
  TITLE     Calcium channel beta 4 (CACNB4): human ortholog of the mouse
            epilepsy gene lethargic
  JOURNAL   Genomics 50 (1), 14-22 (1998)
   PUBMED   9628818
REFERENCE   7  (bases 1 to 7944)
  AUTHORS   Burgess,D.L., Jones,J.M., Meisler,M.H. and Noebels,J.L.
  TITLE     Mutation of the Ca2+ channel beta subunit gene Cchb4 is associated
            with ataxia and seizures in the lethargic (lh) mouse
  JOURNAL   Cell 88 (3), 385-392 (1997)
   PUBMED   9039265
REFERENCE   8  (bases 1 to 7944)
  AUTHORS   Diriong,S., Lory,P., Williams,M.E., Ellis,S.B., Harpold,M.M. and
            Taviaux,S.
  TITLE     Chromosomal localization of the human genes for alpha 1A, alpha 1B,
            and alpha 1E voltage-dependent Ca2+ channel subunits
  JOURNAL   Genomics 30 (3), 605-609 (1995)
   PUBMED   8825650
REFERENCE   9  (bases 1 to 7944)
  AUTHORS   Spacey,S.
  TITLE     Episodic Ataxia Type 2 - RETIRED CHAPTER, FOR HISTORICAL REFERENCE
            ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301674
REFERENCE   10 (bases 1 to 7944)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC079790.6, BC075049.2,
            AC097448.2 and T23939.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 25, 2020 this sequence version replaced NM_000726.4.
            
            Summary: This gene encodes a member of the beta subunit family of
            voltage-dependent calcium channel complex proteins. Calcium
            channels mediate the influx of calcium ions into the cell upon
            membrane polarization and consist of a complex of alpha-1,
            alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various
            versions of each of these subunits exist, either expressed from
            similar genes or the result of alternative splicing. The protein
            encoded by this locus plays an important role in calcium channel
            function by modulating G protein inhibition, increasing peak
            calcium current, controlling the alpha-1 subunit membrane targeting
            and shifting the voltage dependence of activation and inactivation.
            Certain mutations in this gene have been associated with idiopathic
            generalized epilepsy (IGE), juvenile myoclonic epilepsy (JME), and
            episodic ataxia, type 5. [provided by RefSeq, Aug 2016].
            
            Transcript Variant: This variant (2) encodes the longest isoform
            (b). This isoform has a b-type N-terminus.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.243248.1,
                                           SRR1660809.12727.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000539935.7/ ENSP00000438949.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC079790.6         51981-51994         c
            15-1638             BC075049.2         1-1624
            1639-7850           AC097448.2         50377-56588         c
            7851-7944           T23939.1           1-94                c
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q23.3"
     gene            1..7944
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /note="calcium voltage-gated channel auxiliary subunit
                     beta 4"
                     /db_xref="GeneID:785"
                     /db_xref="HGNC:HGNC:1404"
                     /db_xref="MIM:601949"
     exon            1..96
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..1596
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /note="isoform b is encoded by transcript variant 2;
                     dihydropyridine-sensitive L-type, calcium channel beta-4
                     subunit; voltage-dependent L-type calcium channel subunit
                     beta-4; calcium channel voltage-dependent subunit beta 4"
                     /codon_start=1
                     /product="voltage-dependent L-type calcium channel subunit
                     beta-4 isoform b"
                     /protein_id="NP_000717.2"
                     /db_xref="CCDS:CCDS46426.1"
                     /db_xref="GeneID:785"
                     /db_xref="HGNC:HGNC:1404"
                     /db_xref="MIM:601949"
                     /translation="MSSSSYAKNGTADGPHSPTSQVARGTTTRRSRLKRSDGSTTSTS
                     FILRQGSADSYTSRPSDSDVSLEEDREAIRQEREQQAAIQLERAKSKPVAFAVKTNVS
                     YCGALDEDVPVPSTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLRLENI
                     RIQQEQKRGRFHGGKSSGNSSSSLGEMVSGTFRATPTSTAKQKQKVTEHIPPYDVVPS
                     MRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSK
                     RAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDADTINHPAQLIKTSLAPIIVHV
                     KVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDVILDENQLEDACEHLG
                     EYLEAYWRATHTTSSTPMTPLLGRNLGSTALSPYPTAISGLQSQRMRHSNHSTENSPI
                     ERRSLMTSDENYHNERARKSRNRLSSSSQHSRDHYPLVEEDYPDSYQDTYKPHRNRGS
                     PGGYSHDSRHRL"
     exon            97..180
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            181..300
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            301..423
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            424..554
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            555..631
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            632..651
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            652..732
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            733..791
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            792..901
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1053
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1149
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1335
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..7944
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7926..7931
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /note="hexamer: AATGAA"
     polyA_site      7944
                     /gene="CACNB4"
                     /gene_synonym="CAB4; CACNLB4; EA5; EIG9; EJM; EJM4; EJM6"
                     /note="major polyA site"
ORIGIN      
        1 gcacacgggc tggccatgcg gcggctctga acgatgtcct cctcctccta cgccaagaac
       61 gggaccgcgg acgggccgca ctcccccacc tcgcaggtgg cccgaggcac cacaacccgg
      121 aggagcaggt tgaaaagatc cgatggcagc accacttcga ccagcttcat cctcagacag
      181 ggttcagcgg attcctacac aagcaggccg tctgactccg atgtctcttt ggaagaggac
      241 cgggaagcaa ttcgacagga gagagaacag caagcagcta tccagcttga gagagcaaag
      301 tccaaacctg tagcatttgc cgtgaagaca aatgtgagct actgcggcgc cctggacgag
      361 gatgtgcctg ttccaagcac agctatctcc tttgatgcta aagactttct acatattaaa
      421 gagaaatata acaatgattg gtggatagga aggctggtga aagagggctg tgaaattggc
      481 ttcattccaa gtccactcag attggagaac atacggatcc agcaagaaca aaaaagagga
      541 cgttttcacg gagggaaatc aagtggaaat tcttcttcaa gtcttggaga aatggtatct
      601 gggacattcc gagcaactcc cacatcaaca gcaaaacaga agcaaaaagt gacggagcac
      661 attcctcctt acgatgttgt accgtcaatg cgtccggtgg tgttagtggg gccgtcactg
      721 aaaggttacg aggtaacaga catgatgcag aaagccctct ttgatttcct gaagcacagg
      781 tttgatggga ggatttcaat aacgagagtg acagctgaca tttctcttgc taagaggtct
      841 gtcctaaata atcccagcaa gagagcaata attgaacgtt cgaacacccg gtccagctta
      901 gcggaagtac aaagtgaaat tgaaagaatc tttgagttgg caagatcttt gcaactggtt
      961 gttcttgatg cagacaccat caatcaccca gcacaactta taaagacttc cttagcacca
     1021 attattgttc atgtaaaagt ctcatctcca aaggttttac agcggttgat taaatctaga
     1081 ggaaagtcac aaagtaaaca cttgaatgtt caactggtgg cagctgataa acttgcacaa
     1141 tgccccccag aaatgtttga tgttatattg gatgaaaatc agcttgagga tgcatgtgaa
     1201 catctagggg agtacctgga ggcgtactgg cgtgccaccc acacaaccag tagcacaccc
     1261 atgaccccgc tgctgggaag gaatttgggc tccacggcac tctcaccata tcccacagca
     1321 atttctgggt tacagagtca gcgaatgagg cacagcaacc actccacaga gaactctcca
     1381 attgaaagac gaagtctaat gacctctgat gaaaattatc acaatgaaag ggctcggaag
     1441 agtaggaacc gcttgtcttc cagttctcag catagccgag atcattaccc tcttgtggaa
     1501 gaagattacc ctgactcata ccaggacact tacaaacccc ataggaaccg aggatcacct
     1561 gggggatata gccatgactc ccgacatagg ctttgagtct aatgaaacaa aaaatattca
     1621 tctgttgaca atttgccata gcagtgctag gataaaccaa tcatcttaac ttggctaaca
     1681 tagcacagta tttactgtgc taatgggctg ctgtcatttt atgctaagta aggggcaaaa
     1741 aaaaaaatta cattatgccc ttgagtctag atggatatta gatgcccgat catatagata
     1801 tttttaagtg caacatttac atgataacag tacattttgt tttcttcata gatttagaca
     1861 catcaatttg taatttaggg tacttacaag gcacatataa aataatttcc catgctggaa
     1921 attccaaatg accagttcca gttggaacca atttataaac caattcctgt tggaatttgg
     1981 gtgaattgac tagaaagtct acttgagcat gttgtaatca actgaaaaat ctggggggaa
     2041 aaaaaaaaaa ctaataagaa aatcgaaata ccaatagaag ccaaatccag ctttggtatt
     2101 tatttgctgg aagctaaatt tacacttaaa gttggagtgt ataaacattt taatatatgt
     2161 taatgttgcc agaatgactt ttcagactta ccaaatgtat tgatagtgcc aaaaaaaaaa
     2221 tctcagaaat cttaatgcag aaattatata gtctcctcag attttaatat ttttatttct
     2281 ttcctggcac agctgttgta ttcaaagtgt tttgcttttt gtttggtcag tactgttagc
     2341 tcatttgaag ttggcaaaaa caaatacaaa caagcaaaaa acctcaaatt tttttcagca
     2401 gtgagagaga atagtaaaga gaggcccaag catagctggt tatgtgaaat ataaaatggg
     2461 gtgttgttct ccctttaatc aatctgagca caactacata catccaggaa tctgaagtca
     2521 gatagtaagg atgtggtacc aatcctcccg agtgcaatgc taaacacttc caggtgggaa
     2581 ttcatctatt tcacttaaca caaacgcagg gtctgtattt cctgccaaca agattgagta
     2641 tctttccatt gcttctctca gtctctgtct ttgaatatct gaatgttctg gcccttcaac
     2701 aaattcttta aatcatgcta aggaatttcg ttttatgtgg acacactcaa ctttgatatt
     2761 caggagatgt ttttacaaaa tgactaattg aaaaaagttg aaacaaatga ttttaaaagc
     2821 ttgcagtaaa aaataaactc atagattcag caaatatttg ttgactaaaa ttatagcatt
     2881 tttaatgcag aaaattataa agaatttgga gattgagctg ccttatttct aatgaacact
     2941 cctcttcatc ttattaagtc ttcacagttt ttccagtggc tataattatg gaattggtga
     3001 cctatgtttt atattatcaa ggaagcctta gattcactct gatctaagaa attattgaaa
     3061 ttgacccttt ttacacaaat gtctttaaaa aaaaaaactt atttctaaat gtccatagtg
     3121 catgcatttt ctaaaacccc aataagtcca gccataaaag ttcttttctg ctagtacatg
     3181 tcatgaagcc tatttttgaa aataatgata attaatttta tagtatcttc ttttctcccc
     3241 aagcctttca gtattcaggc actcaaagca tgcaccagtt cagtaatctt cttgaccatg
     3301 gaaataagtt ccacattact gatgagttat atatatatct tgaaccccat tacaacctca
     3361 ccacatcccc ctgcatttat gttcagtgtt tgtaccccat tatgaacctg tttagctgat
     3421 aatgaggata cctataattc tgtaagtcat ttcaaatatt tgatacaatt aaaaaatatg
     3481 ggcttagtaa attttaaaag ttaacatgtg atactggctt gtgagattct tctgtcatct
     3541 agtgaagctt ataaaacttt cagttaagac attttaaaag agttatatcc attaggcact
     3601 ttaataaaca tataacctgc cattactgaa tactttgtta gaacacagaa gagcatcttc
     3661 cagcaaacaa aaatatattt attacatgta cagcacatac ttgcatgaga aagaaaacat
     3721 tagccaaaac atttttctat atgctttact ggtttcttgt ttatgcgcat taaagtattt
     3781 aattgcaaag agtgcaccaa ggtgtacaaa tttagactag acagctgttc agctttttct
     3841 gtagtagcat tagcagtttg gtcatttaac tactttaaga tacttacatt ttatggcagg
     3901 tagctgtaac agcatttaaa tatatttcct agtcatcaag attaccatct tctttcaact
     3961 ttttaaaagt cacttgtttt taaaggtttt tcaaagcctt gtttttagtc ttccatgtca
     4021 tgccgataaa gagagtctca ttcaatatct gtgcacaaaa ccaatctatc aattaccatc
     4081 agatgtccat acctgttttg ctttgcattc ttattcaaaa gctgacaatc ttggaaaggg
     4141 tataatgtat attttattga tggatatatt taaatgttta ctttaatttg gaatattctt
     4201 tccccttctt tctgtaataa gtttatcttc ctcaaataag gggatataca tcttattttt
     4261 tttaaagact taagagttta ttgtgtaaat gatacttaaa atctagatat atactaaatg
     4321 aaaagtttta tttttgccaa gcacaccaag cacctttgaa aaatacatac tccatacttt
     4381 gtttttaatt aatttcattt aaagatagca aatgattata agcaagagaa acaagtcttt
     4441 tactgttttg aggctgtttt aacttacagt ataagccaca tactagaatt ctgtaatttc
     4501 agtcacagac atataataat gtggactcat gtgacaatgt ggaaatgtag ttctggaaaa
     4561 aaacgaatgc aatttgtcaa tcctgatgtt aattctcaag ggtataatgt cactctaata
     4621 ttataaccaa caacattatc ttgtttaata tttaaagcaa cacttagaaa tgtttataaa
     4681 tgatgattct ctcttatctt tatacagttc tattggagag aaggttgaaa ttgttcacaa
     4741 tttagatgat ggtctttcct aataagagtc ttaattttgt gactatataa gtcattgcag
     4801 ttagtcttta atgaaatgaa ttttattaat gtaaaacaaa ttctgagaca attatttgtt
     4861 tcctttaaat aatctgctat ttaattatta ttgtcgttgt ttgcaaaatg tatttttgga
     4921 atgttaacct cttttcatac ctgcatactt gaacttgtct tttgtgtggg ggccttaaaa
     4981 ttcataatag gaagtggagg cagtgaaagg aagtaagaaa aggaaattat gctaaaagat
     5041 ggtttgcaaa tttaaattct ttaaatgaac atatcacaaa atgttgttaa gacccaggat
     5101 gtccgttaag aatgtgtctt ggcaacctta atttgcatgt tagtatatat tttcttagtt
     5161 acacagtttt attttggtat ttgcttaaaa aatgtaatac cattgtagaa attaccctat
     5221 gtactgatat atttactacc tcttctctag agaagacaat ctgtctttaa agggaaaaac
     5281 actggtcaat aagtagtgat caaaattatc actattctgg ctaaccttgc tgcaaagcta
     5341 aatacataga ttattaaaat atgccttccc tttagatttt aaactgtttc caaaatgctg
     5401 tttggcaccc tttcatttct tccacgttag tgaagttttc tttgaattgg ttcacagttt
     5461 atcatgaggc aaggttaatt taacaaataa gtcatcatag ggaacaaaac aaactgcaag
     5521 ggaattgcct ttgtgtgcaa cttgttataa tatctagttc taagtctaaa ggagtaaaag
     5581 tccaaagact ttatgtttaa gagcagctgg tttgcaaatt cacattttgt tggtccttga
     5641 cttccacaga gcatgactta gccatttact gggtaaggat atgcataatc ttggactccc
     5701 atgtaattgg aataaacttc atatcaatgt ttcaaataaa tgctatgtac cccttattcc
     5761 ttttacattt ctgcctttgt cttttatgac aatgttgcat gttgtgtttt tttaaattat
     5821 ataaaactca tccttgaatg actgtatgaa tgaaaaattt ataggaaatt atgtaataaa
     5881 atctgtttta ttagtttctc atacctttaa atactgtcaa tactataact gttagtaagt
     5941 tacttactca agctgtgtct atggggcaga gaacagtcct cttctttaaa ataatcatga
     6001 tcaaaccagt caagtcaatc caattagctg ttagacagac aataaggata accttctttt
     6061 tgacttaagc agtcaagtta gtcaaaatag aaaaattggc ttatatgggc acccagaact
     6121 acagcattca aaaattactt ttaaaagttt cttcttttat taaaatggac tttcttaaat
     6181 aaaatacatc ctctgatagc agctatctct ttttacttgt aatgattaat tctaaatgct
     6241 tcatgtaatt gagggacatt atgagaagac tagaaagtat ctcatcagat aatcctaagg
     6301 gctgtaactt ctacttgcca taaacaaagt ataattaaga gtgagtagca attggtttgg
     6361 ggagtcgtag ttatatatga aactttcttt tgtttctcat gatgtatgta aaatgtttgc
     6421 agaaggatgg tagaaaatgt tactaatgaa tttagagtgg tttgggagaa tacactgata
     6481 cttcctcatt tgaaaattat aaggagttac aaggggaact aagaaccaga agtggctggc
     6541 ctctagcttt tcaatacctg ataagtgttc ttggttatgt tttgaacact taaatttctc
     6601 aacaaatggc tatgtctata tttatgtagg ggccaccctt gccctacttg catgccactt
     6661 tgacttttaa ctgaaatcag tgtgaattct gtatgcataa gggatgtaat aaagaggcgg
     6721 gatcattaac gctatatcgt tgcatactat ttttcatatg ccttacaata ttttctggga
     6781 tgttattaaa atgaaagcat tttattgcca aaataaatct atgttgttac ttataatctg
     6841 cattagcagt agaaaaataa tgtagcaaaa acatattgta cagtacaaaa ttataaaata
     6901 tatattttgc caataatata aaaatagatt gaggagaaag aagcacattt gttatagcac
     6961 aaacttataa caaaatccac cgttaaattt ggcagtcata ttttttaaaa gtatgtcgat
     7021 ttagttctaa gttttgtcag atttacttca tctaccatct ttttaacttg ctgcttgata
     7081 cttttccagc aatttatttc atagccttgc aatctgtgga tgtaatttct tttgttttat
     7141 tcccttccat aaaatttatt aaattttatt ttgtgataac actcatacca tattttctcc
     7201 tcttatttct tctctttcaa taacaacagc agaaagtcac agcaagtgag ctcatagggc
     7261 tcctctgaat tttggacatg tgtgagattt ttgtattttt cacagcctcg agtcagaatg
     7321 atcaataaag atgtcccaac cacaaaaaat tatattagtt agggattctt taatttgttt
     7381 tgtactgaat actactttga aacataagtt aaagccatta gctgtgtttt tgtagggatg
     7441 gtgggaagga ataaaaaata aatgtattaa cataaaacat actaaaacag aaatatggca
     7501 aaatgatgta aaaagatttt tcctactatt ctgagggctg ttgaaatccc aggctattgc
     7561 tatatctcca ttgacaaagt gggataaaaa atgcctttgt caaataattt tttttctttc
     7621 tttaaatttt agttatttat tttaataatt tgtccccttt tatttttctt atctaggtat
     7681 gtgtagcatt ctacaaggca tgctgggtaa aatatactat gcaaatgtgt acagtgctgc
     7741 atgttttgtt ctccctctgc tttagtggct ttaaagaaaa aaacgtggtg ttatttttgt
     7801 gaataaaaac attaacaaag aaaatgcaga gtgcatgcat agagtatcct catttctatg
     7861 gatttgtttt atggaattta aatgtgtaca aaacaactgc attacttttt ttcttttata
     7921 taataaatga aaaaaacact gaaa
//
