LOCUS       NM_172364               5285 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens calcium voltage-gated channel auxiliary subunit
            alpha2delta 4 (CACNA2D4), mRNA.
ACCESSION   NM_172364
VERSION     NM_172364.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Schlegel,D.K., Glasauer,S.M.K., Mateos,J.M., Barmettler,G.,
            Ziegler,U. and Neuhauss,S.C.F.
  TITLE     A New Zebrafish Model for CACNA2D4-Dysfunction
  JOURNAL   Invest Ophthalmol Vis Sci 60 (15), 5124-5135 (2019)
   PUBMED   31834350
  REMARK    GeneRIF: We find that increasing the expression of Cav at the
            synaptic membrane is an evolutionarily conserved function of
            Cacna2d4b. Yet, since both paralogs participate in cone synaptic
            transmission, we propose partial subfunctionalization in zebrafish.
            Similar to human patients, our double KO zebrafish model shows mild
            cone dysfunction, which was not associated with signs of retinal
            degeneration.
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Chiang,J.P., Luo,H., Duan,J., Ekstein,J. and Hirsch,Y.
  TITLE     Founder Ashkenazi Jewish mutations of large deletion in the
            inherited retinal dystrophy genes
  JOURNAL   Ophthalmic Genet 39 (1), 135-136 (2018)
   PUBMED   28726569
  REMARK    GeneRIF: We have confirmed the TRPM1 36,445 bp deletion is a
            founder mutation in the Ashkenazi-Jewish (AJ) population with a
            carrier rate of 1 in 50. We have also confirmed the 35,741 bp
            deletion in the CACNA2D4 gene is a founder mutation in the AJ
            population with a carrier rate of 1 in 56.
REFERENCE   3  (bases 1 to 5285)
  AUTHORS   Ba-Abbad,R., Arno,G., Carss,K., Stirrups,K., Penkett,C.J.,
            Moore,A.T., Michaelides,M., Raymond,F.L., Webster,A.R. and
            Holder,G.E.
  TITLE     Mutations in CACNA2D4 Cause Distinctive Retinal Dysfunction in
            Humans
  JOURNAL   Ophthalmology 123 (3), 668-71 (2016)
   PUBMED   26560832
  REMARK    GeneRIF: This report describes a distinctive ERG phenotype,
            predominantly involving the cone pathways, in 2 unrelated patients
            from different ethnic backgrounds with homozygous mutations in
            CACNA2D4 and normal retinal imaging
REFERENCE   4  (bases 1 to 5285)
  AUTHORS   Sung,Y.J., de las Fuentes,L., Schwander,K.L., Simino,J. and
            Rao,D.C.
  TITLE     Gene-smoking interactions identify several novel blood pressure
            loci in the Framingham Heart Study
  JOURNAL   Am J Hypertens 28 (3), 343-354 (2015)
   PUBMED   25189868
REFERENCE   5  (bases 1 to 5285)
  AUTHORS   Deng,X., Sabino,E.C., Cunha-Neto,E., Ribeiro,A.L., Ianni,B.,
            Mady,C., Busch,M.P. and Seielstad,M.
  CONSRTM   REDSII Chagas Study Group from the NHLBI Retrovirus Epidemiology
            Donor Study-II Component International
  TITLE     Genome wide association study (GWAS) of Chagas cardiomyopathy in
            Trypanosoma cruzi seropositive subjects
  JOURNAL   PLoS One 8 (11), e79629 (2013)
   PUBMED   24324551
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5285)
  AUTHORS   McGue,M., Zhang,Y., Miller,M.B., Basu,S., Vrieze,S., Hicks,B.,
            Malone,S., Oetting,W.S. and Iacono,W.G.
  TITLE     A genome-wide association study of behavioral disinhibition
  JOURNAL   Behav Genet 43 (5), 363-373 (2013)
   PUBMED   23942779
REFERENCE   7  (bases 1 to 5285)
  AUTHORS   Van Den Bossche,M.J., Strazisar,M., De Bruyne,S., Bervoets,C.,
            Lenaerts,A.S., De Zutter,S., Nordin,A., Norrback,K.F., Goossens,D.,
            De Rijk,P., Green,E.K., Grozeva,D., Mendlewicz,J., Craddock,N.,
            Sabbe,B.G., Adolfsson,R., Souery,D. and Del-Favero,J.
  TITLE     Identification of a CACNA2D4 deletion in late onset bipolar
            disorder patients and implications for the involvement of
            voltage-dependent calcium channels in psychiatric disorders
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 159B (4), 465-475 (2012)
   PUBMED   22488967
  REMARK    GeneRIF: A rare, partial deletion of 35.7 kb in CACNA2D4 in two
            unrelated late onset bipolar I patients and in one control
            individual, were identified.
REFERENCE   8  (bases 1 to 5285)
  AUTHORS   Wycisk,K.A., Zeitz,C., Feil,S., Wittmer,M., Forster,U.,
            Neidhardt,J., Wissinger,B., Zrenner,E., Wilke,R., Kohl,S. and
            Berger,W.
  TITLE     Mutation in the auxiliary calcium-channel subunit CACNA2D4 causes
            autosomal recessive cone dystrophy
  JOURNAL   Am J Hum Genet 79 (5), 973-977 (2006)
   PUBMED   17033974
REFERENCE   9  (bases 1 to 5285)
  AUTHORS   Qin,N., Yagel,S., Momplaisir,M.L., Codd,E.E. and D'Andrea,M.R.
  TITLE     Molecular cloning and characterization of the human voltage-gated
            calcium channel alpha(2)delta-4 subunit
  JOURNAL   Mol Pharmacol 62 (3), 485-496 (2002)
   PUBMED   12181424
  REMARK    GeneRIF: Calcium channel alpha(2)delta-4 subunit has limited
            distribution in special cell types of the pituitary, adrenal gland,
            colon, and fetal liver.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF516695.1, AC005342.1,
            BX537437.1 and AC005343.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_172364.4.
            
            Summary: This gene encodes a member of the alpha-2/delta subunit
            family, a protein in the voltage-dependent calcium channel complex.
            Calcium channels mediate the influx of calcium ions into the cell
            upon membrane polarization and consist of a complex of alpha-1,
            alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various
            versions of each of these subunits exist, either expressed from
            similar genes or the result of alternative splicing. Research on a
            highly similar protein in rabbit suggests the protein described in
            this record is cleaved into alpha-2 and delta subunits. Alternate
            transcriptional splice variants of this gene have been observed but
            have not been thoroughly characterized. [provided by RefSeq, Jul
            2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX537437.1, AF516695.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000382722.10/ ENSP00000372169.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1157              AF516695.1         185-1341
            1158-1172           AC005342.1         10539-10553         c
            1173-2306           BX537437.1         1226-2359
            2307-2814           AF516695.1         2491-2998
            2815-3279           BX537437.1         2868-3332
            3280-4407           AF516695.1         3464-4591
            4408-5285           AC005343.1         82923-83800
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.33"
     gene            1..5285
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /note="calcium voltage-gated channel auxiliary subunit
                     alpha2delta 4"
                     /db_xref="GeneID:93589"
                     /db_xref="HGNC:HGNC:20202"
                     /db_xref="MIM:608171"
     exon            1..406
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..3593
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /note="voltage-gated calcium channel alpha(2)delta-4
                     subunit; calcium channel, voltage-dependent, alpha 2/delta
                     subunit 4; voltage-gated calcium channel subunit
                     alpha-2/delta-4; alpha2delta-4"
                     /codon_start=1
                     /product="voltage-dependent calcium channel subunit
                     alpha-2/delta-4"
                     /protein_id="NP_758952.4"
                     /db_xref="CCDS:CCDS44785.1"
                     /db_xref="GeneID:93589"
                     /db_xref="HGNC:HGNC:20202"
                     /db_xref="MIM:608171"
                     /translation="MVCGCSALLPLPNPRPTMPATPNFLANPSSSSRWIPLQPMPVAW
                     AFVQKTSALLWLLLLGTSLSPAWGQAKIPLETVKLWADTFGGDLYNTVTKYSGSLLLQ
                     KKYKDVESSLKIEEVDGLELVRKFSEDMENMLRRKVEAVQNLVEAAEEADLNHEFNES
                     LVFDYYNSVLINERDEKGNFVELGAEFLLESNAHFSNLPVNTSISSVQLPTNVYNKDP
                     DILNGVYMSEALNAVFVENFQRDPTLTWQYFGSATGFFRIYPGIKWTPDENGVITFDC
                     RNRGWYIQAATSPKDIVILVDVSGSMKGLRMTIAKHTITTILDTLGENDFINIIAYND
                     YVHYIEPCFKGILVQADRDNREHFKLLVEELMVKGVGVVDQALREAFQILKQFQEAKQ
                     GSLCNQAIMLISDGAVEDYEPVFEKYNWPDCKVRVFTYLIGREVSFADRMKWIACNNK
                     GYYTQISTLADTQENVMEYLHVLSRPMVINHDHDIIWTEAYMDSKLLSSQAQSLTLLT
                     TVAMPVFSKKNETRSHGILLGVVGSDVALRELMKLAPRYKLGVHGYAFLNTNNGYILS
                     HPDLRPLYREGKKLKPKPNYNSVDLSEVEWEDQAESLRTAMINRETGTLSMDVKVPMD
                     KGKRVLFLTNDYFFTDISDTPFSLGVVLSRGHGEYILLGNTSVEEGLHDLLHPDLALA
                     GDWIYCITDIDPDHRKLSQLEAMIRFLTRKDPDLECDEELVREVLFDAVVTAPMEAYW
                     TALALNMSEESEHVVDMAFLGTRAGLLRSSLFVGSEKVSDRKFLTPEDEASVFTLDRF
                     PLWYRQASEHPAGSFVFNLRWAEGPESAGEPMVVTASTAVAVTVDKRTAIAAAAGVQM
                     KLEFLQRKFWAATRQCSTVDGPCTQSCEDSDLDCFVIDNNGFILISKRSRETGRFLGE
                     VDGAVLTQLLSMGVFSQVTMYDYQAMCKPSSHHHSAAQPLVSPISAFLTATRWLLQEL
                     VLFLLEWSVWGSWYDRGAEAKSVFHHSHKHKKQDPLQPCDTEYPVFVYQPAIREANGI
                     VECGPCQKVFVVQQIPNSNLLLLVTDPTCDCSIFPPVLQEATEVKYNASVKCDRMRSQ
                     KLRRRPDSCHAFHPEENAQDCGGASDTSASPPLLLLPVCAWGLLPQLLR"
     exon            407..488
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            489..605
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            606..665
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            666..828
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            829..960
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1021
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1172
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1247
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1337
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1451
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1530
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1664
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1742
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1823
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1898
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..1985
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2057
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2119
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2187
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2233
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2331
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2425
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2521
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..2649
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2650..2730
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2792
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..2837
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..2900
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2901..2971
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2972..3047
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            3048..3100
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            3101..3153
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            3154..3174
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            3175..3292
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            3293..3405
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            3406..3488
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            3489..5285
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5264..5269
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /note="hexamer: AATAAA"
     polyA_site      5285
                     /gene="CACNA2D4"
                     /gene_synonym="RCD4"
                     /note="major polyA site"
ORIGIN      
        1 agtccctgtc tccgaggcct gcttgctagg tggggcgact cttctgtgag ctgcagctgt
       61 ggggcagaga gaagccctcc ccacccagga ctgagaggct ggagacccca ggctcgccaa
      121 ggcacggaag acaaaggcct ggggcgtcct ggggtctggg aggaaggcag acagagctca
      181 tggtctgtgg ctgctctgcc ctccttcccc tccccaaccc caggcccacc atgcctgcaa
      241 ctcccaactt cctcgcaaac cccagctcca gcagccgctg gattcccctc cagccaatgc
      301 ccgtggcctg ggcctttgtg cagaagacct cggccctcct gtggctgctg cttctaggca
      361 cctccctgtc ccctgcgtgg ggacaggcca agattcctct ggaaacagtg aagctatggg
      421 ctgacacctt cggcggggac ctgtataaca ctgtgaccaa atactcaggc tctctcttgc
      481 tgcagaagaa gtacaaggat gtggagtcca gtctgaagat cgaggaggtg gatggcttgg
      541 agctggtgag gaagttctca gaggacatgg agaacatgct gcggaggaaa gtcgaggcgg
      601 tccagaatct ggtggaagct gccgaggagg ccgacctgaa ccacgaattc aatgaatccc
      661 tggtgttcga ctattacaac tcggtcctga tcaacgagag ggacgagaag ggcaacttcg
      721 tggagctggg cgccgagttc ctcctggagt ccaatgctca cttcagcaac ctgccggtga
      781 acacctccat cagcagcgtg cagctgccca ccaacgtgta caacaaagac ccagatattt
      841 taaatggagt ctacatgtct gaagccttga atgctgtctt cgtggagaac ttccagagag
      901 acccaacgtt gacctggcaa tattttggca gtgcaactgg attcttcagg atctatccag
      961 gtataaaatg gacacctgat gagaatggag tcattacttt tgactgccga aaccgcggct
     1021 ggtacattca agctgctact tctcccaagg acatagtgat tttggtggac gtgagcggca
     1081 gtatgaaggg gctgaggatg actattgcca agcacaccat caccaccatc ttggacaccc
     1141 tgggggagaa tgacttcatt aatatcatag cgtacaatga ctacgtccat tacatcgagc
     1201 cttgttttaa agggatcctc gtccaggcgg accgagacaa tcgagagcat ttcaaactgc
     1261 tggtggagga gttgatggtc aaaggtgtgg gggtcgtgga ccaagccctg agagaagcct
     1321 tccagatcct gaagcagttc caagaggcca agcaaggaag cctctgcaac caggccatca
     1381 tgctcatcag cgacggcgcc gtggaggact acgagccggt gtttgagaag tataactggc
     1441 cagactgtaa ggtccgagtt ttcacttacc tcattgggag agaagtgtct tttgctgacc
     1501 gcatgaagtg gattgcatgc aacaacaaag gctactacac gcagatctca acgctggcgg
     1561 acacccagga gaacgtgatg gaatacctgc acgtgctcag ccgccccatg gtcatcaacc
     1621 acgaccacga catcatctgg acagaggcct acatggacag caagctcctc agctcgcagg
     1681 ctcagagcct gacactgctc accactgtgg ccatgccagt cttcagcaag aagaacgaaa
     1741 cgcgatccca tggcattctc ctgggtgtgg tgggctcaga tgtggccctg agagagctga
     1801 tgaagctggc gccccggtac aagcttggag tgcacggata cgcctttctg aacaccaaca
     1861 atggctacat cctctcccat cccgacctcc ggcccctgta cagagagggg aagaaactaa
     1921 aacccaaacc taactacaac agtgtggatc tctccgaagt ggagtgggaa gaccaggctg
     1981 aatctctgag aacagccatg atcaataggg aaacaggtac tctctcgatg gatgtgaagg
     2041 ttccgatgga taaagggaag cgagttcttt tcctgaccaa tgactacttc ttcacggaca
     2101 tcagcgacac ccctttcagt ttgggggtgg tgctgtcccg gggccacgga gaatacatcc
     2161 ttctggggaa cacgtctgtg gaagaaggcc tgcatgactt gcttcaccca gacctggccc
     2221 tggccggtga ctggatctac tgcatcacag atattgaccc agaccaccgg aagctcagcc
     2281 agctagaggc catgatccgc ttcctcacca ggaaggaccc agacctggag tgtgacgagg
     2341 agctggtccg ggaggtgctg tttgacgcgg tggtgacagc ccccatggaa gcctactgga
     2401 cagcgctggc cctcaacatg tccgaggagt ctgaacacgt ggtggacatg gccttcctgg
     2461 gcacccgggc tggcctcctg agaagcagct tgttcgtggg ctccgagaag gtctccgaca
     2521 ggaagttcct gacacctgag gacgaggcca gcgtgttcac cctggaccgc ttcccgctgt
     2581 ggtaccgcca ggcctcagag catcctgctg gcagcttcgt cttcaacctc cgctgggcag
     2641 aaggaccaga aagtgcgggt gaacccatgg tggtgacggc aagcacagct gtggcggtga
     2701 ccgtggacaa gaggacagcc attgctgcag ccgcgggcgt ccaaatgaag ctggaattcc
     2761 tccagcgcaa attctgggcg gcaacgcggc agtgcagcac tgtggatggg ccgtgcacac
     2821 agagctgcga ggacagtgat ctggactgct tcgtcatcga caacaacggg ttcattctga
     2881 tctccaagag gtcccgagag acgggaagat ttctggggga ggtggatggt gctgtcctga
     2941 cccagctgct cagcatgggg gtgttcagcc aagtgactat gtatgactat caggccatgt
     3001 gcaaaccctc gagtcaccac cacagtgcag cccagcccct ggtcagccca atttctgcct
     3061 tcttgacggc gaccaggtgg ctgctgcagg agctggtgct gttcctgctg gagtggagtg
     3121 tctggggctc ctggtacgac agaggggccg aggccaaaag tgtcttccat cactcccaca
     3181 aacacaagaa gcaggacccg ctgcagccct gcgacacgga gtaccccgtg ttcgtgtacc
     3241 agccggccat ccgggaggcc aacgggatcg tggagtgcgg gccctgccag aaggtatttg
     3301 tggtgcagca gattcccaac agtaacctcc tcctcctggt gacagacccc acctgtgact
     3361 gcagcatctt cccaccagtg ctgcaggagg cgacagaagt caaatataat gcctctgtca
     3421 aatgtgaccg gatgcgctcc cagaagctcc gccggcgacc agactcctgc cacgccttcc
     3481 atccagagga gaatgcccag gactgcggcg gcgcctcgga cacctcagcc tcgccgcccc
     3541 tactcctgct gcctgtgtgt gcctgggggc tactgcccca actcctgcgg tgacaccacc
     3601 cagcctgacc tgtgttttgg caaggtgatc cttccagagc catcccaaaa agtcagcact
     3661 gacatgggat gcagctaact gcagttgggt cgcccccagg ccaacgctcc tctcaatcct
     3721 gggctggtgg cccctggctc cggagaatgc tggatggaac aggaaaccaa tcacctggca
     3781 ccactttcaa gatgcttcat ggtgcccggt accatctgcc ctaggtctca acatgagcat
     3841 acttctgacc taaccttcct gtctcctctt cgggaagcca gcgtgagctc agcttggacc
     3901 aagacaaaat aatttagttc ttcctgtact ccagagtcca gacccagcca agaaagggtc
     3961 agttgtttct gaccctttct gtcggagtgg tctctggtag aacccaagga cttctgggta
     4021 ctgagaagca gcagcagaat gaggccaaat gcagagatga ggctaaggca agaatatgcc
     4081 ccaactaaag catagattcc ccaaagtgag gctcatggtg ggaggccact caccttccta
     4141 gctgctgctc gaaaaggttt tgactgtgtt ggggtggggg ttgggtaagg gaatggtcaa
     4201 gactgagaaa ggaatgaaat ccattcagga aatatcgaca gggctacacg tgatgtcccc
     4261 aaactgctgc tattgaagaa cttcccaaaa cttctttaca aagccctaaa ggaaagtttg
     4321 catctatgaa aagccaatag gctgagacat ccaattgctg catggaaatt gatgtacatt
     4381 caggggacgg caaaaatagc tgtaaaatag tgaaaaagag cagtggttgt gctcttttct
     4441 ggccaatgat ttacaaaaga atctacttga ctctgtccct ggagtgaaat ccttagggtt
     4501 ggaacttgtg ggaacattcc aacttgctaa gcagggtcca ctgggaggga agctctatct
     4561 gggaactcac ccccagcgca cacacatctc ccccagggtc ccaaggcccc gcagcttcct
     4621 cccccgacca aaccccaaga cctggatccc aggagacaac agtctccaca gtgagagcaa
     4681 cattaagggc aaagccatgg agaaatgtgg gagaggccgg cctcaaatct ttccatttaa
     4741 caaaccccag tgatgggtat ggacagcatg cagggctttt ggggcgcttc cccccgctcc
     4801 tccatcaccc tcagcctcca cacttcaaag ttcaagttca aagctgttca agtttcctac
     4861 cagcaaatag ccctaacttg cctctagagt aggccaaatg ccaactctgt aaaacacact
     4921 tacattatcg gttacagaat gtcactctta ccgtcatgtc ttgcaacaac cctgtgaggg
     4981 cagtattaat gcccccttac agcagaagac actgcagctc gaagacagct taagtggcag
     5041 aataatgcta gaacagctaa ggtttacatg taccaaataa catgtttcag ctcattccat
     5101 cctcacaaca gccccctgaa agtgggtact atcattagtc ccatgttata gaaactgcag
     5161 cagagttgaa aattgcctcc aaattaccgg aagagtgtat gaagattgaa tgtgatgtat
     5221 tcacgtaaca tgcttgaaac tgcctggcat atactaaacg ctaaataaat acatgctaac
     5281 tgcga
//
