LOCUS       NM_018398               3789 bp    mRNA    linear   PRI 28-JUL-2024
DEFINITION  Homo sapiens calcium voltage-gated channel auxiliary subunit
            alpha2delta 3 (CACNA2D3), mRNA.
ACCESSION   NM_018398
VERSION     NM_018398.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Liu,G., Lv,Y., Wang,Y., Xu,Z., Chen,L., Chen,S., Xie,W., Feng,Y.,
            Liu,J., Bai,Y., He,Y., Li,X. and Wu,Q.
  TITLE     Remote ischemic preconditioning reduces mitochondrial apoptosis
            mediated by calpain 1 activation in myocardial ischemia-reperfusion
            injury through calcium channel subunit Cacna2d3
  JOURNAL   Free Radic Biol Med 212, 80-93 (2024)
   PUBMED   38151212
  REMARK    GeneRIF: Remote ischemic preconditioning reduces mitochondrial
            apoptosis mediated by calpain 1 activation in myocardial
            ischemia-reperfusion injury through calcium channel subunit
            Cacna2d3.
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Yu,L.L., Hu,B.W., Huang,H.X., Yu,B., Xiao,Q., Lv,Q.L., Luo,C.H.,
            Guo,C.X., Li,J.G., Xie,X.X. and Yin,J.Y.
  TITLE     A two-stage genome-wide association study identifies novel germline
            genetic variations in CACNA2D3 associated with radiotherapy
            response in nasopharyngeal carcinoma
  JOURNAL   J Transl Med 21 (1), 11 (2023)
   PUBMED   36624463
  REMARK    GeneRIF: A two-stage genome-wide association study identifies novel
            germline genetic variations in CACNA2D3 associated with
            radiotherapy response in nasopharyngeal carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3789)
  AUTHORS   Auxerre-Plantie,E., Nakamori,M., Renaud,Y., Huguet,A., Choquet,C.,
            Dondi,C., Miquerol,L., Takahashi,M.P., Gourdon,G., Junion,G.,
            Jagla,T., Zmojdzian,M. and Jagla,K.
  TITLE     Straightjacket/alpha2delta3 deregulation is associated with cardiac
            conduction defects in myotonic dystrophy type 1
  JOURNAL   Elife 8, e51114 (2019)
   PUBMED   31829940
  REMARK    GeneRIF: The authors show that ventricular alpha2delta3 expression
            is low in healthy mice and humans, but significantly elevated in
            ventricular muscles from DM1 patients with conduction defects.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3789)
  AUTHORS   Smedler,E., Bergen,S.E., Song,J. and Landen,M.
  TITLE     Genes, biomarkers, and clinical features associated with the course
            of bipolar disorder
  JOURNAL   Eur Neuropsychopharmacol 29 (10), 1152-1160 (2019)
   PUBMED   31378653
  REMARK    GeneRIF: CNS sensitisation was associated with a common genetic
            variant near the calcium channel gene CACNA2D3 at genome-wide
            significance.
REFERENCE   5  (bases 1 to 3789)
  AUTHORS   You,H.L., Huang,W.T., Liu,T.T., Weng,S.W. and Eng,H.L.
  TITLE     Mutations of candidate tumor suppressor genes at chromosome 3p in
            intrahepatic cholangiocarcinoma
  JOURNAL   Exp Mol Pathol 103 (3), 249-254 (2017)
   PUBMED   29122566
  REMARK    GeneRIF: Nine of the 32 (28.1%) iCCA patients had gene mutations at
            chromosome 3p, totaling 11 mutations across five genes. Those
            included five (15.6%) BAP1 mutations, two each (6.3%) of CACNA2D3
            and RASSF1 mutations, and one each (3.1%) of ATG7 and PLCD1
            mutations. Six (18.8%) cases had concurrent loss of chromosome 3p
            and gene mutations.
REFERENCE   6  (bases 1 to 3789)
  AUTHORS   Uhl,G.R., Liu,Q.R., Drgon,T., Johnson,C., Walther,D., Rose,J.E.,
            David,S.P., Niaura,R. and Lerman,C.
  TITLE     Molecular genetics of successful smoking cessation: convergent
            genome-wide association study results
  JOURNAL   Arch Gen Psychiatry 65 (6), 683-693 (2008)
   PUBMED   18519826
  REMARK    GeneRIF: Clinical trial and genome-wide association study of
            gene-disease association, gene-environment interaction, and
            pharmacogenomic / toxicogenomic. (HuGE Navigator)
REFERENCE   7  (bases 1 to 3789)
  AUTHORS   Abo-Dalo,B., Kim,H.G., Roes,M., Stefanova,M., Higgins,A., Shen,Y.,
            Mundlos,S., Quade,B.J., Gusella,J.F. and Kutsche,K.
  TITLE     Extensive molecular genetic analysis of the 3p14.3 region in
            patients with Zimmermann-Laband syndrome
  JOURNAL   Am J Med Genet A 143A (22), 2668-2674 (2007)
   PUBMED   17937436
  REMARK    GeneRIF: did not detect submicroscopic deletion or duplication nor
            sequence alteration in either CACNA2D3 or WNT5A in ZLS-affected
            individuals
REFERENCE   8  (bases 1 to 3789)
  AUTHORS   Qin,N., Yagel,S., Momplaisir,M.L., Codd,E.E. and D'Andrea,M.R.
  TITLE     Molecular cloning and characterization of the human voltage-gated
            calcium channel alpha(2)delta-4 subunit
  JOURNAL   Mol Pharmacol 62 (3), 485-496 (2002)
   PUBMED   12181424
  REMARK    GeneRIF: This publication primarily discusses the alpha2/delta 4
            subunit, but also contains cDNA data relating to the gene described
            in this record.
REFERENCE   9  (bases 1 to 3789)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
REFERENCE   10 (bases 1 to 3789)
  AUTHORS   Hanke,S., Bugert,P., Chudek,J. and Kovacs,G.
  TITLE     Cloning a calcium channel alpha2delta-3 subunit gene from a
            putative tumor suppressor gene region at chromosome 3p21.1 in
            conventional renal cell carcinoma
  JOURNAL   Gene 264 (1), 69-75 (2001)
   PUBMED   11245980
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC115282.2, AC133010.2,
            AC138059.3, AC137673.2, AC092057.2, AC109584.2, AC092797.2 and
            AC104298.2.
            
            On May 17, 2019 this sequence version replaced NM_018398.2.
            
            Summary: This gene encodes a member of the alpha-2/delta subunit
            family, a protein in the voltage-dependent calcium channel complex.
            Calcium channels mediate the influx of calcium ions into the cell
            upon membrane polarization and consist of a complex of alpha-1,
            alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various
            versions of each of these subunits exist, either expressed from
            similar genes or the result of alternative splicing. Research on a
            highly similar protein in rabbit suggests the protein described in
            this record is cleaved into alpha-2 and delta subunits. Alternate
            transcriptional splice variants of this gene have been observed but
            have not been thoroughly characterized. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.38772.1,
                                           SRR1660809.163023.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145544, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000474759.6/ ENSP00000419101.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-284               AC115282.2         124231-124514
            285-366             AC115282.2         125192-125273
            367-483             AC133010.2         88226-88342
            484-543             AC133010.2         154499-154558
            544-706             AC138059.3         133262-133424
            707-838             AC137673.2         88076-88207
            839-899             AC137673.2         95071-95131
            900-1050            AC137673.2         95230-95380
            1051-1125           AC137673.2         107079-107153
            1126-1215           AC092057.2         141994-142083       c
            1216-1329           AC092057.2         127629-127742       c
            1330-1408           AC092057.2         17193-17271         c
            1409-1542           AC092057.2         5519-5652           c
            1543-1560           AC109584.2         71150-71167
            1561-1632           AC109584.2         91456-91527
            1633-1713           AC109584.2         92865-92945
            1714-1788           AC109584.2         100690-100764
            1789-1872           AC109584.2         125836-125919
            1873-1944           AC109584.2         133315-133386
            1945-2006           AC109584.2         133647-133708
            2007-2074           AC109584.2         135093-135160
            2075-2120           AC109584.2         139578-139623
            2121-2218           AC109584.2         139786-139883
            2219-2312           AC109584.2         142256-142349
            2313-2408           AC109584.2         145652-145747
            2409-2530           AC109584.2         151046-151167
            2531-2611           AC109584.2         154085-154165
            2612-2673           AC092797.2         174627-174688
            2674-2718           AC092797.2         175977-176021
            2719-2781           AC092797.2         190785-190847
            2782-2852           AC092797.2         193860-193930
            2853-2928           AC104298.2         76397-76472
            2929-2981           AC104298.2         79424-79476
            2982-3037           AC104298.2         81022-81077
            3038-3149           AC104298.2         89840-89951
            3150-3262           AC104298.2         145079-145191
            3263-3345           AC104298.2         145411-145493
            3346-3789           AC104298.2         145748-146191
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.1-p14.3"
     gene            1..3789
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /note="calcium voltage-gated channel auxiliary subunit
                     alpha2delta 3"
                     /db_xref="GeneID:55799"
                     /db_xref="HGNC:HGNC:15460"
                     /db_xref="MIM:606399"
     exon            1..284
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..3438
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /note="calcium channel, voltage-dependent, alpha 2/delta 3
                     subunit; calcium channel alpha2-delta3 subunit;
                     voltage-dependent calcium channel subunit alpha-2/delta-3;
                     voltage-gated calcium channel subunit alpha-2/delta-3;
                     calcium channel, voltage-dependent, alpha 2/delta subunit
                     3; alpha2delta-3"
                     /codon_start=1
                     /product="voltage-dependent calcium channel subunit
                     alpha-2/delta-3 precursor"
                     /protein_id="NP_060868.2"
                     /db_xref="CCDS:CCDS54598.1"
                     /db_xref="GeneID:55799"
                     /db_xref="HGNC:HGNC:15460"
                     /db_xref="MIM:606399"
                     /translation="MAGPGSPRRASRGASALLAAALLYAALGDVVRSEQQIPLSVVKL
                     WASAFGGEIKSIAAKYSGSQLLQKKYKEYEKDVAIEEIDGLQLVKKLAKNMEEMFHKK
                     SEAVRRLVEAAEEAHLKHEFDADLQYEYFNAVLINERDKDGNFLELGKEFILAPNDHF
                     NNLPVNISLSDVQVPTNMYNKDPAIVNGVYWSESLNKVFVDNFDRDPSLIWQYFGSAK
                     GFFRQYPGIKWEPDENGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIAK
                     QTVSSILDTLGDDDFFNIIAYNEELHYVEPCLNGTLVQADRTNKEHFREHLDKLFAKG
                     IGMLDIALNEAFNILSDFNHTGQGSICSQAIMLITDGAVDTYDTIFAKYNWPDRKVRI
                     FTYLIGREAAFADNLKWMACANKGFFTQISTLADVQENVMEYLHVLSRPKVIDQEHDV
                     VWTEAYIDSTLPQAQKLTDDQGPVLMTTVAMPVFSKQNETRSKGILLGVVGTDVPVKE
                     LLKTIPKYKLGIHGYAFAITNNGYILTHPELRLLYEEGKKRRKPNYSSVDLSEVEWED
                     RDDVLRNAMVNRKTGKFSMEVKKTVDKGKRVLVMTNDYYYTDIKGTPFSLGVALSRGH
                     GKYFFRGNVTIEEGLHDLEHPDVSLADEWSYCNTDLHPEHRHLSQLEAIKLYLKGKEP
                     LLQCDKELIQEVLFDAVVSAPIEAYWTSLALNKSENSDKGVEVAFLGTRTGLSRINLF
                     VGAEQLTNQDFLKAGDKENIFNADHFPLWYRRAAEQIPGSFVYSIPFSTGPVNKSNVV
                     TASTSIQLLDERKSPVVAAVGIQMKLEFFQRKFWTASRQCASLDGKCSISCDDETVNC
                     YLIDNNGFILVSEDYTQTGDFFGEIEGAVMNKLLTMGSFKRITLYDYQAMCRANKESS
                     DGAHGLLDPYNAFLSAVKWIMTELVLFLVEFNLCSWWHSDMTAKAQKLKQTLEPCDTE
                     YPAFVSERTIKETTGNIACEDCSKSFVIQQIPSSNLFMVVVDSSCLCESVAPITMAPI
                     EIRYNESLKCERLKAQKIRRRPESCHGFHPEENARECGGAPSLQAQTVLLLLPLLLML
                     FSR"
     exon            285..366
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            367..483
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            484..543
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            544..706
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            707..838
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            839..899
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1050
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1125
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1215
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1329
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1408
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1542
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1560
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1632
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..1713
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1788
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..1872
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1944
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2006
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2074
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2120
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2121..2218
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2312
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2408
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2530
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2611
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2612..2673
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2674..2718
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2719..2781
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2782..2852
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..2928
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..2981
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            2982..3037
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3149
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            3150..3262
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            3263..3345
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3789
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3767..3772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /note="hexamer: AATAAA"
     polyA_site      3789
                     /gene="CACNA2D3"
                     /gene_synonym="HSA272268"
                     /note="major polyA site"
ORIGIN      
        1 gctccttttt ctgctcccca agtgagccgg gcgcgcgaga ggcaggcggg gcggcgcgga
       61 gcggagcagg cagccccgcg cgctcgccca ccgcccgctc cgcgcagctc cccgcggccg
      121 ctctcgtcgc cgccgcagcg ggcgcgtcgg agggagccca gcatggccgg gccgggctcg
      181 ccgcgccgcg cgtcccgggg ggcctcggcg cttctcgctg ccgcgcttct ctacgccgcg
      241 ctgggggacg tggtgcgctc ggagcagcag ataccgctct ccgtggtgaa gctctgggcc
      301 tcggcttttg gtggggagat aaaatccatt gctgctaagt actccggttc ccagcttctg
      361 caaaagaaat acaaagagta tgagaaagac gttgccatag aagaaattga tggcctccaa
      421 ctggtaaaga agctggcaaa gaacatggaa gagatgtttc acaagaagtc tgaggccgtc
      481 aggcgtctgg tggaggctgc agaagaagca cacctgaaac atgaatttga tgcagactta
      541 cagtatgaat acttcaatgc tgtgctgata aatgaaaggg acaaagacgg gaattttttg
      601 gagctgggaa aggaattcat cttagcccca aatgaccatt ttaataattt gcctgtgaac
      661 atcagtctaa gtgacgtcca agtaccaacg aacatgtaca acaaagaccc tgcaattgtc
      721 aatggggttt attggtctga atctctaaac aaagtttttg tagataactt tgaccgtgac
      781 ccatctctca tatggcagta ctttggaagt gcaaagggct tttttaggca gtatccgggg
      841 attaaatggg aaccagatga gaatggagtc attgccttcg actgcaggaa ccgaaaatgg
      901 tacatccagg cagcaacttc tccgaaagac gtggtcattt tagttgacgt cagtggcagc
      961 atgaaaggac tccgtctgac tatcgcgaag caaacagtct catccatttt ggatacactt
     1021 ggggatgatg acttcttcaa cataattgct tataatgagg agcttcacta tgtggaacct
     1081 tgcctgaatg gaactttggt gcaagccgac aggacaaaca aagagcactt cagggagcat
     1141 ctggacaaac ttttcgccaa aggaattgga atgttggata tagctctgaa tgaggccttc
     1201 aacattctga gtgatttcaa ccacacggga caaggaagta tctgcagtca ggccatcatg
     1261 ctcataactg atggggcggt ggacacctat gatacaatct ttgcaaaata caattggcca
     1321 gatcgaaagg ttcgcatctt cacatacctc attggacgag aggctgcgtt tgcagacaat
     1381 ctaaagtgga tggcctgtgc caacaaagga ttttttaccc agatctccac cttggctgat
     1441 gtgcaggaga atgtcatgga ataccttcac gtgcttagcc ggcccaaagt catcgaccag
     1501 gagcatgatg tggtgtggac cgaagcttac attgacagca ctctccctca ggcacaaaag
     1561 ctgactgatg atcagggccc cgtcctgatg accactgtag ccatgcctgt gtttagtaag
     1621 cagaacgaaa ccagatcgaa gggcattctt ctgggagtgg ttggcacaga tgtcccagtg
     1681 aaagaacttc tgaagaccat ccccaaatac aagttaggga ttcacggtta tgcctttgca
     1741 atcacaaata atggatatat cctgacgcat ccggaactca ggctgctgta cgaagaagga
     1801 aaaaagcgaa ggaaacctaa ctatagtagc gttgacctct ctgaggtgga gtgggaagac
     1861 cgagatgacg tgttgagaaa tgctatggtg aatcgaaaga cggggaagtt ttccatggag
     1921 gtgaagaaga cagtggacaa agggaaacgg gttttggtga tgacaaatga ctactattat
     1981 acagacatca agggtactcc tttcagttta ggtgtggcgc tttccagagg tcatgggaaa
     2041 tatttcttcc gagggaatgt aaccatcgaa gaaggcctgc atgacttaga acatcccgat
     2101 gtgtccttgg cagatgaatg gtcctactgc aacactgacc tacaccctga gcaccgccat
     2161 ctgtctcagt tagaagcgat taagctctac ctaaaaggca aagaacctct gctccagtgt
     2221 gataaagaat tgatccaaga agtccttttt gacgcggtgg tgagtgcccc cattgaagcg
     2281 tattggacca gcctggccct caacaaatct gaaaattctg acaagggcgt ggaggttgcc
     2341 ttcctcggca ctcgcacggg cctctccaga atcaacctgt ttgtcggggc tgagcagctc
     2401 accaatcagg acttcctgaa agctggcgac aaggagaaca tttttaacgc agaccatttc
     2461 cctctctggt accgaagagc cgctgagcag attccaggga gcttcgtcta ctcgatccca
     2521 ttcagcactg gaccagtcaa taaaagcaat gtggtgacag caagtacatc catccagctc
     2581 ctggatgaac ggaaatctcc tgtggtggca gctgtaggca ttcagatgaa acttgaattt
     2641 ttccaaagga agttctggac tgccagcaga cagtgtgctt ccctggatgg caaatgctcc
     2701 atcagctgtg atgatgagac tgtgaattgt tacctcatag acaataatgg atttattttg
     2761 gtgtctgaag actacacaca gactggagac ttttttggtg agatcgaggg agctgtgatg
     2821 aacaaattgc taacaatggg ctcctttaaa agaattaccc tttatgacta ccaagccatg
     2881 tgtagagcca acaaggaaag cagcgatggc gcccatggcc tcctggatcc ttataatgcc
     2941 ttcctctctg cagtaaaatg gatcatgaca gaacttgtct tgttcctggt ggaatttaac
     3001 ctctgcagtt ggtggcactc cgatatgaca gctaaagccc agaaattgaa acagaccctg
     3061 gagccttgtg atactgaata tccagcattc gtctctgagc gcaccatcaa ggagactaca
     3121 gggaatattg cttgtgaaga ctgctccaag tcctttgtca tccagcaaat cccaagcagc
     3181 aacctgttca tggtggtggt ggacagcagc tgcctctgtg aatctgtggc ccccatcacc
     3241 atggcaccca ttgaaatcag gtataatgaa tcccttaagt gtgaacgtct aaaggcccag
     3301 aagatcagaa ggcgcccaga atcttgtcat ggcttccatc ctgaggagaa tgcaagggag
     3361 tgtgggggtg cgccgagtct ccaagcccag acagtcctcc ttctgctccc tctgcttttg
     3421 atgctcttct caaggtgaca ctgactgaga tgttctctta ctgactgaga tgttctcttg
     3481 gcatgctaaa tcatggataa actgtgaacc aaaatatggt gcaacatacg agacatgaat
     3541 atagtccaac catcagcatc tcatcatgat tttaaactgt gcgtgatata aactcttaaa
     3601 gatatgttga caaaaagtta tctatcatct ttttactttg ccagtcatgc aaatgtgagt
     3661 ttgccacatg ataatcaccc ttcatcagaa atgggaccgc aagtggtagg cagtgtccct
     3721 tctgcttgaa acctattgaa accaatttaa aactgtgtac tttttaaata aagtatatta
     3781 aaatcataa
//
