LOCUS       NM_001005505            5702 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens calcium voltage-gated channel auxiliary subunit
            alpha2delta 2 (CACNA2D2), transcript variant 1, mRNA.
ACCESSION   NM_001005505
VERSION     NM_001005505.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Warnier,M., Roudbaraki,M., Derouiche,S., Delcourt,P., Bokhobza,A.,
            Prevarskaya,N. and Mariot,P.
  TITLE     CACNA2D2 promotes tumorigenesis by stimulating cell proliferation
            and angiogenesis
  JOURNAL   Oncogene 34 (42), 5383-5394 (2015)
   PUBMED   25619833
  REMARK    GeneRIF: show that the action of alpha2delta2 on tumor development
            occurs not only through a stimulation of proliferation
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Pippucci,T., Parmeggiani,A., Palombo,F., Maresca,A., Angius,A.,
            Crisponi,L., Cucca,F., Liguori,R., Valentino,M.L., Seri,M. and
            Carelli,V.
  TITLE     A novel null homozygous mutation confirms CACNA2D2 as a gene
            mutated in epileptic encephalopathy
  JOURNAL   PLoS One 8 (12), e82154 (2013)
   PUBMED   24358150
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5702)
  AUTHORS   Rhodin,A., Gronbladh,A., Ginya,H., Nilsson,K.W., Rosenblad,A.,
            Zhou,Q., Enlund,M., Hallberg,M., Gordh,T. and Nyberg,F.
  TITLE     Combined analysis of circulating beta-endorphin with gene
            polymorphisms in OPRM1, CACNAD2 and ABCB1 reveals correlation with
            pain, opioid sensitivity and opioid-related side effects
  JOURNAL   Mol Brain 6, 8 (2013)
   PUBMED   23402298
  REMARK    GeneRIF: The investigation of single nucleotide polymorphisms in
            three genes that have a functional impact on the opioid response:
            OPRM1, ABCB1 and the calcium channel complex subunit CACNA2D2, is
            reported.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5702)
  AUTHORS   Edvardson,S., Oz,S., Abulhijaa,F.A., Taher,F.B., Shaag,A.,
            Zenvirt,S., Dascal,N. and Elpeleg,O.
  TITLE     Early infantile epileptic encephalopathy associated with a high
            voltage gated calcium channelopathy
  JOURNAL   J Med Genet 50 (2), 118-123 (2013)
   PUBMED   23339110
REFERENCE   5  (bases 1 to 5702)
  AUTHORS   Mitra,S., Mazumder Indra,D., Basu,P.S., Mondal,R.K., Roy,A.,
            Roychoudhury,S. and Panda,C.K.
  TITLE     Alterations of RASSF1A in premalignant cervical lesions: clinical
            and prognostic significance
  JOURNAL   Mol Carcinog 51 (9), 723-733 (2012)
   PUBMED   21809394
  REMARK    GeneRIF: CACNA2D2 deletion/methylation is associated with cervical
            disease progression.
REFERENCE   6  (bases 1 to 5702)
  AUTHORS   Li,Z., Taylor,C.P., Weber,M., Piechan,J., Prior,F., Bian,F.,
            Cui,M., Hoffman,D. and Donevan,S.
  TITLE     Pregabalin is a potent and selective ligand for alpha(2)delta-1 and
            alpha(2)delta-2 calcium channel subunits
  JOURNAL   Eur J Pharmacol 667 (1-3), 80-90 (2011)
   PUBMED   21651903
REFERENCE   7  (bases 1 to 5702)
  AUTHORS   Angeloni,D., Duh,F.M., Wei,M.F., Johnson,B.E. and Lerman,M.I.
  TITLE     A G-to-A single nucleotide polymorphism in intron 2 of the human
            CACNA2D2 gene that maps at 3p21.3
  JOURNAL   Mol Cell Probes 15 (2), 125-127 (2001)
   PUBMED   11292331
REFERENCE   8  (bases 1 to 5702)
  AUTHORS   Lerman,M.I. and Minna,J.D.
  TITLE     The 630-kb lung cancer homozygous deletion region on human
            chromosome 3p21.3: identification and evaluation of the resident
            candidate tumor suppressor genes. The International Lung Cancer
            Chromosome 3p21.3 Tumor Suppressor Gene Consortium
  JOURNAL   Cancer Res 60 (21), 6116-6133 (2000)
   PUBMED   11085536
REFERENCE   9  (bases 1 to 5702)
  AUTHORS   Gao,B., Sekido,Y., Maximov,A., Saad,M., Forgacs,E., Latif,F.,
            Wei,M.H., Lerman,M., Lee,J.H., Perez-Reyes,E., Bezprozvanny,I. and
            Minna,J.D.
  TITLE     Functional properties of a new voltage-dependent calcium channel
            alpha(2)delta auxiliary subunit gene (CACNA2D2)
  JOURNAL   J Biol Chem 275 (16), 12237-12242 (2000)
   PUBMED   10766861
REFERENCE   10 (bases 1 to 5702)
  AUTHORS   Hobom,M., Dai,S., Marais,E., Lacinova,L., Hofmann,F. and
            Klugbauer,N.
  TITLE     Neuronal distribution and functional characterization of the
            calcium channel alpha2delta-2 subunit
  JOURNAL   Eur J Neurosci 12 (4), 1217-1226 (2000)
   PUBMED   10762351
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL450422.2, AF040709.1,
            Z84492.2 and BF373910.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001005505.2.
            
            Summary: Calcium channels mediate the entry of calcium ions into
            the cell upon membrane polarization. This gene encodes the
            alpha-2/delta subunit of the voltage-dependent calcium channel
            complex. The complex consists of the main channel-forming subunit
            alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The
            auxiliary subunits function in the assembly and membrane
            localization of the complex, and modulate calcium currents and
            channel activation/inactivation kinetics. The subunit encoded by
            this gene undergoes post-translational cleavage to yield the
            extracellular alpha2 peptide and a membrane-anchored delta
            polypeptide. This subunit is a receptor for the antiepileptic drug,
            gabapentin. Mutations in this gene are associated with early
            infantile epileptic encephalopathy. Single nucleotide polymorphisms
            in this gene are correlated with increased sensitivity to opioid
            drugs. Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Mar 2014].
            
            Transcript Variant: This variant (1) lacks an alternate in-frame
            exon in the central coding region and uses an alternate in-frame
            splice site in the 3' coding region compared to variant 3. The
            encoded isoform (a) is shorter than isoform c.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF040709.1, BC152438.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AL450422.2         25425-25461
            38-1659             AF040709.1         7-1628
            1660-1660           Z84492.2           8593-8593
            1661-5513           AF040709.1         1630-5482
            5514-5550           Z84492.2           23830-23866
            5551-5637           BF373910.1         77-163              c
            5638-5691           Z84492.2           23954-24007
            5692-5702           BF373910.1         14-24               c
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..5702
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /note="calcium voltage-gated channel auxiliary subunit
                     alpha2delta 2"
                     /db_xref="GeneID:9254"
                     /db_xref="HGNC:HGNC:1400"
                     /db_xref="MIM:607082"
     exon            1..417
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..3649
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; gene 26; alpha 2 delta calcium channel subunit;
                     voltage-gated calcium channel subunit alpha-2/delta-2;
                     calcium channel, voltage-dependent, alpha 2/delta subunit
                     2; alpha2delta-2"
                     /codon_start=1
                     /product="voltage-dependent calcium channel subunit
                     alpha-2/delta-2 isoform a precursor"
                     /protein_id="NP_001005505.1"
                     /db_xref="CCDS:CCDS63647.1"
                     /db_xref="GeneID:9254"
                     /db_xref="HGNC:HGNC:1400"
                     /db_xref="MIM:607082"
                     /translation="MAVPARTCGASRPGPARTARPWPGCGPHPGPGTRRPTSGPPRPL
                     WLLLPLLPLLAAPGASAYSFPQQHTMQHWARRLEQEVDGVMRIFGGVQQLREIYKDNR
                     NLFEVQENEPQKLVEKVAGDIESLLDRKVQALKRLADAAENFQKAHRWQDNIKEEDIV
                     YYDAKADAELDDPESEDVERGSKASTLRLDFIEDPNFKNKVNYSYAAVQIPTDIYKGS
                     TVILNELNWTEALENVFMENRRQDPTLLWQVFGSATGVTRYYPATPWRAPKKIDLYDV
                     RRRPWYIQGASSPKDMVIIVDVSGSVSGLTLKLMKTSVCEMLDTLSDDDYVNVASFNE
                     KAQPVSCFTHLVQANVRNKKVFKEAVQGMVAKGTTGYKAGFEYAFDQLQNSNITRANC
                     NKMIMMFTDGGEDRVQDVFEKYNWPNRTVRVFTFSVGQHNYDVTPLQWMACANKGYYF
                     EIPSIGAIRINTQEYLDVLGRPMVLAGKEAKQVQWTNVYEDALGLGLVVTGTLPVFNL
                     TQDGPGEKKNQLILGVMGIDVALNDIKRLTPNYTLGANGYVFAIDLNGYVLLHPNLKP
                     QTTNFREPVTLDFLDAELEDENKEEIRRSMIDGNKGHKQIRTLVKSLDERYIDEVTRN
                     YTWVPIRSTNYSLGLVLPPYSTFYLQANLSDQILQVKYFEFLLPSSFESEGHVFIAPR
                     EYCKDLNASDNNTEFLKNFIELMEKVTPDSKQCNNFLLHNLILDTGITQQLVERVWRD
                     QDLNTYSLLAVFAATDGGITRVFPNKAAEDWTENPEPFNASFYRRSLDNHGYVFKPPH
                     QDALLRPLELENDTVGILVSTAVELSLGRRTLRPAVVGVKLDLEAWAEKFKVLASNRT
                     HQDQPQKCGPNSHCEMDCEVNNEDLLCVLIDDGGFLVLSNQNHQWDQVGRFFSEVDAN
                     LMLALYNNSFYTRKESYDYQAACAPQPPGNLGAAPRGVFVPTVADFLNLAWWTSAAAW
                     SLFQQLLYGLIYHSWFQADPAEAEGSPETRESSCVMKQTQYYFGSVNASYNAIIDCGN
                     CSRLFHAQRLTNTNLLFVVAEKPLCSQCEAGRLLQKETHCPADGPEQCELVQRPRYRR
                     GPHICFDYNATEDTSDCGRGASFPPSLGVLVSLQLLLLLGLPPRPQPQVLVHASRRL"
     exon            418..499
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            500..616
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            617..676
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            677..721
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            722..863
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            864..995
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1053
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1104
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1204
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1363
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1471
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1550
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1600
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1690
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1762
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1763..1837
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1912
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..1984
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2056
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2118
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2195
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..2256
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2257..2354
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2355..2445
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2508
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2509..2612
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2613..2711
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2712..2800
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2848
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2849..2920
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            2921..3073
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            3074..3126
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3182
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3309
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            3310..3419
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3508
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     exon            3509..5702
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5412..5417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /note="hexamer: AAGAAA"
     polyA_site      5427
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /note="major polyA site"
     regulatory      5491..5496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /note="hexamer: AATAAT"
     polyA_site      5516
                     /gene="CACNA2D2"
                     /gene_synonym="CACNA2D; CASVDD"
                     /note="major polyA site"
ORIGIN      
        1 agagcgcttg gagccagcgc tgcagggaga tagcagcgcg cagcccgcag aggcgctgcg
       61 gcccgtgcag ccccggaggc ccctcgcgga gaaggcggcg gcggaggaga ggccgagtta
      121 ccgcccgccg cccgcgcccc cccaaccccg ccgccgccgc cgccgccgcc actgcccccc
      181 ctccccgcgg cgccgcatct tgaatggaaa catggcggtg ccggctcgga cctgcggcgc
      241 ctctcggccc ggcccagcgc ggactgcgcg cccctggccc ggctgcggcc cccaccctgg
      301 ccccggcacc cggcgcccga cgtccgggcc cccgcgcccg ctgtggctgc tgctgccgct
      361 tctaccgctg ctcgccgccc ccggcgcctc tgcctacagc ttcccccagc agcacacgat
      421 gcagcactgg gcccggcgtc tggagcagga ggtcgacggc gtgatgcgga tttttggagg
      481 cgtccagcag ctccgtgaga tttacaagga caaccggaac ctgttcgagg tacaggagaa
      541 tgagcctcag aagttggtgg agaaggtggc aggggacatt gagagccttc tggacaggaa
      601 ggtgcaggcc ctgaagagac tggctgatgc tgcagagaac ttccagaaag cacaccgctg
      661 gcaggacaac atcaaggagg aagacatcgt gtactatgac gccaaggctg acgctgagct
      721 ggacgaccct gagagtgagg atgtggaaag ggggtctaag gccagcaccc taaggctgga
      781 cttcatcgag gacccaaact tcaagaacaa ggtcaactat tcatacgcgg ctgtacagat
      841 ccctacggac atctacaaag gctccactgt catcctcaat gagctcaact ggacagaggc
      901 cctggagaat gtgttcatgg aaaaccgcag acaagacccc acactgctgt ggcaggtctt
      961 cggcagcgcc acaggagtca ctcgctacta cccggccacc ccgtggcgag cccccaagaa
     1021 gatcgacctg tacgatgtcc gaaggagacc ctggtatatc cagggggcct cgtcacccaa
     1081 agacatggtc atcatcgtgg atgtgagtgg cagtgtgagc ggcctgaccc tgaagctgat
     1141 gaagacatct gtctgcgaga tgctggacac gctgtctgat gatgactatg tgaatgtggc
     1201 ctcgttcaac gagaaggcac agcctgtgtc atgcttcaca cacctggtgc aggccaatgt
     1261 gcgcaacaag aaggtgttca aggaagctgt gcagggcatg gtggccaagg gcaccacagg
     1321 ctacaaggcc ggctttgagt atgcctttga ccagctgcag aactccaaca tcactcgggc
     1381 caactgcaac aagatgatca tgatgttcac ggatggtggt gaggaccgcg tgcaggacgt
     1441 ctttgagaag tacaattggc caaaccggac ggtgcgcgtg tttactttct ccgtggggca
     1501 gcataactat gacgtcacac cgctgcagtg gatggcctgt gccaacaaag gctactattt
     1561 tgagatccct tccatcggag ccatccgcat caacacacag gaatatctag atgtgttggg
     1621 caggcccatg gtgctggcag gcaaggaggc caagcaggtg cagtggacca acgtgtatga
     1681 ggatgcactg ggactggggt tggtggtaac agggaccctc cctgttttca acctgacaca
     1741 ggatggccct ggggaaaaga agaaccagct gatcctgggc gtgatgggca ttgacgtggc
     1801 tctgaatgac atcaagaggc tgacccccaa ctacacgctt ggagccaacg gctatgtgtt
     1861 tgccattgac ctgaacggct acgtgttgct gcaccccaat ctcaagcccc agaccaccaa
     1921 cttccgggag cctgtgactc tggacttcct ggatgcggag ctagaggatg agaacaagga
     1981 agagatccgt cggagcatga ttgatggcaa caagggccac aagcagatca gaacgttggt
     2041 caagtccctg gatgagaggt acatagatga ggtgacacgg aactacacct gggtgcctat
     2101 aaggagcact aactacagcc tggggctggt gctcccaccc tacagcacct tctacctcca
     2161 agccaatctc agtgaccaga tcctgcaggt caagtatttt gagttcctgc tccccagcag
     2221 ctttgagtct gaaggacacg ttttcattgc tcccagagag tactgcaagg acctgaatgc
     2281 ctcagacaac aacaccgagt tcctgaaaaa ctttattgag ctcatggaga aagtgactcc
     2341 agactccaag cagtgcaaca acttccttct gcacaacctg atcttggaca cgggcatcac
     2401 gcagcagctg gtagagcgtg tgtggaggga ccaggatctc aacacgtaca gcctactggc
     2461 cgtgttcgct gccacagacg gtggcatcac ccgagtcttc cccaacaagg cagctgagga
     2521 ctggacagag aaccctgagc ccttcaatgc cagcttctac cgccgcagcc tggataacca
     2581 cggttatgtc ttcaagcccc cacaccagga tgccctgtta aggccgctgg agctggagaa
     2641 tgacactgtg ggcatcctcg tcagcacagc tgtggagctc agcctaggca ggcgcacact
     2701 gaggccagca gtggtgggcg tcaagctgga cctagaggct tgggctgaga agttcaaggt
     2761 gctagccagc aaccgtaccc accaagacca gcctcagaag tgcggcccca acagccactg
     2821 tgagatggac tgcgaggtta acaatgagga cttactctgt gtcctcattg atgatggagg
     2881 attcctggtg ctgtcaaacc agaaccatca gtgggaccag gtgggcaggt tcttcagtga
     2941 ggtggatgcc aacctgatgc tggcactcta caataactcc ttctacaccc gcaaggagtc
     3001 ctatgactat caggcagcct gtgcccctca gccccctggc aacctgggtg ctgcaccccg
     3061 gggtgtcttt gtgcccaccg ttgcagattt ccttaacctg gcctggtgga cctctgctgc
     3121 cgcctggtcc ctgttccagc agcttctcta cggcctcatc taccacagct ggttccaagc
     3181 agaccccgcg gaggccgagg ggagccccga gacgcgcgag agcagctgcg tcatgaaaca
     3241 gacccagtac tacttcggct cggtaaacgc ctcctacaac gccatcatcg actgcggaaa
     3301 ctgctccagg ctgttccacg cgcagagact gaccaacacc aatcttctct ttgtggtggc
     3361 cgagaagccg ctgtgcagcc agtgcgaggc tggccggctg ctgcagaagg agacgcactg
     3421 cccagcggac ggcccggagc agtgtgagct agtgcagaga ccgcgatacc ggagaggccc
     3481 gcacatctgc ttcgactaca acgcgacaga agatacctca gactgtggcc gcggggcctc
     3541 cttcccgccg tcgctgggcg tcctggtctc cctgcaactg ctgctcctcc tgggcctgcc
     3601 gccccggccg cagcctcaag tcctcgtcca cgcctctcgc cgcctctgag caccctgccc
     3661 caccccacct ccactcccac ctcacccggc ctcttcgcct ttcccaccct cctgccccac
     3721 actccccgcc ttagagcctc gtccctccct cactgaagga cctgagctgg ccaggccctg
     3781 agagtctggt ctgcgccttg ggatggggag tcccaaagcg ggacgccgca ggtgtttggc
     3841 acccaaatca catctcacct ccgaactgtt caagtgtccc cagacccttc ttgcctgctg
     3901 ggctcccccc agtgggatgg gacagggagg ccacacgcac tggtgccaaa accaggcctc
     3961 tgctgccgcc cttcctggag gctgcctatg ttggggggga ccctgcctca gctgacccgg
     4021 cctctctgcc ccacccaagc ccaaacttgg tttctgtgag aatagtggag gaaggtgaga
     4081 tggccagttt gaagcctgtg cctcccagct taaatcctag caggagagag gctctggggc
     4141 agcccccatg ggctcctgcc cctttcaggc ctacagccac atccccaagc ccaccaggtg
     4201 tcaggatagt cacagtgata ccagttcaga cactacccca tatacacctg gaacattgag
     4261 gatggaaact ggactcacat tcgacatacc ccactgggca cacgcacaaa cacacacact
     4321 atggggtggg gtgggtgtag gggcttacaa agccttacac agggcgaggg gttggtggga
     4381 gggttggcac ctgcacactc catctcctgc tcaccacctg cctctaatct gagctgcagc
     4441 ctggctggtc ctcccatttc taaagctgaa tgtcaaacag tgccaaatgc tggggcaggg
     4501 ggtgaagaac cctctgtccc acccctagcc accagtgtcc tccaagtgcc ccctcacctc
     4561 tccaggtgct cattgtaacc atttctcact agtgtcaggc ccccagtggg accacatgcc
     4621 actgcctgca cctttcggca gaggaacccc caccagacat caccctttgc cttagcaggg
     4681 gtgactttgt ctctcctggc tgggccatcc ttccgccaat ctggccctta cacactcagg
     4741 cctgtgccca ctccctatct ccttcccacc cctacacaca cactccctgc ttgcaggagg
     4801 ccaaactgtc cctcccttgc tgaacacaca cacacacaca cacacacagg tggggactgg
     4861 gcacagctct tcacaccatt cattctggtc atttccccca aaggcatccc agcctggggg
     4921 ccagtgggga actgagggca aggggatata gtgatggggc tcagatggac tgggaggagg
     4981 gggagggtga tgcattaatt aatggcttcg ttaattaatg tcatgttgct tgtcgctttc
     5041 tcagtgtgtg tgtgtggtcc atgcccactg ctggtgccag ggtgggtgtc catgtgcacc
     5101 cggcctggat gccagctgtg tccttcgggg gcgtgcgtgt aactgtagtg tagtcaggtg
     5161 ctcaatggag aatataaaca tatacagaaa aatatatatt ttaagtttaa aaaacagaaa
     5221 aacagacaaa acaatcccca tcaggtagct gtctaacccc cagctgggtc taatccttct
     5281 cattacccac ccgacctggc tgcccctcac cttgggctgg gggactgggg ggccatttcc
     5341 ttttctctgc cctttttttg ttgttctatt ttgtacagac aagttggaaa aacaacagcg
     5401 acaaaaaagt caagaaactt tgtaaaatat cgtgtgtgtg attccttgta aaatattttc
     5461 aaatggttta ttacagaaga tcagttatta aataatgttc atattttcac ttcaaatggt
     5521 tcccatccac tgtatcagct tgggggtgag gactgggtag ctatgaagac agttggccaa
     5581 gacctcagag tcccacttag tgctctgcag ggggtgaaga ccatggtagc ccaggtccct
     5641 gtcaaccaca gggcatggca cttgcttcca ccagttataa taggtgccag gcccttgaca
     5701 ta
//
