LOCUS       NM_000722               7542 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens calcium voltage-gated channel auxiliary subunit
            alpha2delta 1 (CACNA2D1), transcript variant 1, mRNA.
ACCESSION   NM_000722
VERSION     NM_000722.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Inoue,H., Shiozaki,A., Kosuga,T., Shimizu,H., Kudou,M., Arita,T.,
            Konishi,H., Komatsu,S., Kuriu,Y., Kubota,T., Fujiwara,H.,
            Morinaga,Y., Konishi,E. and Otsuji,E.
  TITLE     CACNA2D1 regulates the progression and influences the
            microenvironment of colon cancer
  JOURNAL   J Gastroenterol 59 (7), 556-571 (2024)
   PUBMED   38536483
  REMARK    GeneRIF: CACNA2D1 regulates the progression and influences the
            microenvironment of colon cancer.
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Chen,Z., Mondal,A., Abderemane-Ali,F., Jang,S., Niranjan,S.,
            Montano,J.L., Zaro,B.W. and Minor,D.L. Jr.
  TITLE     EMC chaperone-CaV structure reveals an ion channel assembly
            intermediate
  JOURNAL   Nature 619 (7969), 410-419 (2023)
   PUBMED   37196677
  REMARK    GeneRIF: EMC chaperone-CaV structure reveals an ion channel
            assembly intermediate.
REFERENCE   3  (bases 1 to 7542)
  AUTHORS   Dahimene,S., von Elsner,L., Holling,T., Mattas,L.S., Pickard,J.,
            Lessel,D., Pilch,K.S., Kadurin,I., Pratt,W.S., Zhulin,I.B., Dai,H.,
            Hempel,M., Ruzhnikov,M.R.Z., Kutsche,K. and Dolphin,A.C.
  TITLE     Biallelic CACNA2D1 loss-of-function variants cause early-onset
            developmental epileptic encephalopathy
  JOURNAL   Brain 145 (8), 2721-2729 (2022)
   PUBMED   35293990
  REMARK    GeneRIF: Biallelic CACNA2D1 loss-of-function variants cause
            early-onset developmental epileptic encephalopathy.
REFERENCE   4  (bases 1 to 7542)
  AUTHORS   Schleithoff,L., Mehrke,G., Reutlinger,B. and Lehmann-Horn,F.
  TITLE     Genomic structure and functional expression of a human
            alpha(2)/delta calcium channel subunit gene (CACNA2)
  JOURNAL   Genomics 61 (2), 201-209 (1999)
   PUBMED   10534405
REFERENCE   5  (bases 1 to 7542)
  AUTHORS   Iles,D.E., Lehmann-Horn,F., Scherer,S.W., Tsui,L.C., Olde
            Weghuis,D., Suijkerbuijk,R.F., Heytens,L., Mikala,G., Schwartz,A.,
            Ellis,F.R. et al.
  TITLE     Localization of the gene encoding the alpha 2/delta-subunits of the
            L-type voltage-dependent calcium channel to chromosome 7q and
            analysis of the segregation of flanking markers in malignant
            hyperthermia susceptible families
  JOURNAL   Hum Mol Genet 3 (6), 969-975 (1994)
   PUBMED   7951247
REFERENCE   6  (bases 1 to 7542)
  AUTHORS   Powers,P.A., Scherer,S.W., Tsui,L.C., Gregg,R.G. and Hogan,K.
  TITLE     Localization of the gene encoding the alpha 2/delta subunit
            (CACNL2A) of the human skeletal muscle voltage-dependent Ca2+
            channel to chromosome 7q21-q22 by somatic cell hybrid analysis
  JOURNAL   Genomics 19 (1), 192-193 (1994)
   PUBMED   8188232
REFERENCE   7  (bases 1 to 7542)
  AUTHORS   Brust,P.F., Simerson,S., McCue,A.F., Deal,C.R., Schoonmaker,S.,
            Williams,M.E., Velicelebi,G., Johnson,E.C., Harpold,M.M. and
            Ellis,S.B.
  TITLE     Human neuronal voltage-dependent calcium channels: studies on
            subunit structure and role in channel assembly
  JOURNAL   Neuropharmacology 32 (11), 1089-1102 (1993)
   PUBMED   8107964
REFERENCE   8  (bases 1 to 7542)
  AUTHORS   Brugada,R., Campuzano,O., Sarquella-Brugada,G., Brugada,P.,
            Brugada,J. and Hong,K.
  TITLE     Brugada Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301690
REFERENCE   9  (bases 1 to 7542)
  AUTHORS   Williams,M.E., Feldman,D.H., McCue,A.F., Brenner,R., Velicelebi,G.,
            Ellis,S.B. and Harpold,M.M.
  TITLE     Structure and functional expression of alpha 1, alpha 2, and beta
            subunits of a novel human neuronal calcium channel subtype
  JOURNAL   Neuron 8 (1), 71-84 (1992)
   PUBMED   1309651
REFERENCE   10 (bases 1 to 7542)
  AUTHORS   Ellis,S.B., Williams,M.E., Ways,N.R., Brenner,R., Sharp,A.H.,
            Leung,A.T., Campbell,K.P., McKenna,E., Koch,W.J., Hui,A.,
            Schwartz,A. and Harpold,M.M.
  TITLE     Sequence and expression of mRNAs encoding the alpha 1 and alpha 2
            subunits of a DHP-sensitive calcium channel
  JOURNAL   Science 241 (4873), 1661-1664 (1988)
   PUBMED   2458626
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP258070.1, BC051339.1,
            M76559.1, BG055272.1, AL707206.1, AC006145.2, AL833141.1 and
            AI924415.1.
            
            On Nov 22, 2018 this sequence version replaced NM_000722.3.
            
            Summary: The preproprotein encoded by this gene is cleaved into
            multiple chains that comprise the alpha-2 and delta subunits of the
            voltage-dependent calcium channel complex. Calcium channels mediate
            the influx of calcium ions into the cell upon membrane
            polarization. Mutations in this gene can cause cardiac
            deficiencies, including Brugada syndrome and short QT syndrome.
            Alternate splicing results in multiple transcript variants, some of
            which may lack the delta subunit portion. [provided by RefSeq, Nov
            2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.256576.1,
                                           SRR1660805.49818.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356860.8/ ENSP00000349320.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-135               BP258070.1         29-163
            136-1197            BC051339.1         75-1136
            1198-1474           M76559.1           914-1190
            1475-1643           BG055272.1         134-302
            1644-3431           M76559.1           1360-3147
            3432-3611           AL707206.1         473-652
            3612-5709           AC006145.2         58762-60859         c
            5710-7539           AL833141.1         1-1830
            7540-7542           AI924415.1         13-15               c
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.11"
     gene            1..7542
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /note="calcium voltage-gated channel auxiliary subunit
                     alpha2delta 1"
                     /db_xref="GeneID:781"
                     /db_xref="HGNC:HGNC:1399"
                     /db_xref="MIM:114204"
     exon            1..413
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     CDS             319..3594
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; calcium channel, L type, alpha 2 polypeptide;
                     dihydropyridine-sensitive L-type, calcium channel
                     alpha-2/delta subunit; voltage-gated calcium channel
                     subunit alpha-2/delta-1; calcium channel,
                     voltage-dependent, alpha 2/delta subunit 1; alpha2delta-1"
                     /codon_start=1
                     /product="voltage-dependent calcium channel subunit
                     alpha-2/delta-1 isoform 1 preproprotein"
                     /protein_id="NP_000713.2"
                     /db_xref="CCDS:CCDS5598.1"
                     /db_xref="GeneID:781"
                     /db_xref="HGNC:HGNC:1399"
                     /db_xref="MIM:114204"
                     /translation="MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDL
                     VTLAKTASGVNQLVDIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAL
                     EAEKVQAAHQWREDFASNEVVYYNAKDDLDPEKNDSEPGSQRIKPVFIEDANFGRQIS
                     YQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKKNREEDPSLLWQVFGSATGLARYY
                     PASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSV
                     SEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYK
                     KGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFNKYNKDKKVRVFTFSVGQH
                     NYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVY
                     LDALELGLVITGTLPVFNITGQFENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCP
                     NGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGE
                     KTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQAR
                     SKKGKMKDSETLKPDNFEESGYTFIAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNN
                     PSCNADLINRVLLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENW
                     QENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVEIYIQGKLLKPA
                     VVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYT
                     NQIGRFFGEIDPSLMRHLVNISVYAFNKSYDYQSVCEPGAAPKQGAGHRSAYVPSVAD
                     ILQIGWWATAAAWSILQQFLLSLTFPRLLEAVEMEDDDFTASLSKQSCITEQTQYFFD
                     NDSKSFSGVLDCGNCSRIFHGEKLMNTNLIFIMVESKGTCPCDTRLLIQAEQTSDGPN
                     PCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWYIIGIQFLLLWLVSGSTH
                     RLL"
     exon            414..495
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            496..612
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            613..672
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            673..714
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            715..844
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            845..976
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1046
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1097
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1197
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1356
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1461
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1540
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1590
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1680
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1758
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1833
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1908
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1909..1980
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2052
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2114
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2191
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2192..2212
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2273
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..2371
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..2459
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2522
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2626
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2627..2713
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2714..2781
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2782..2820
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2821..2892
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            2893..3045
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            3046..3098
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..3154
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3284
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            3285..3394
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3477
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     exon            3478..7542
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7518..7523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /note="hexamer: AATAAA"
     polyA_site      7542
                     /gene="CACNA2D1"
                     /gene_synonym="CACNA2; CACNL2A; CCHL2A; DEE110; LINC01112;
                     lncRNA-N3"
                     /note="major polyA site"
ORIGIN      
        1 agcgagagcg cgcgagcgcc ggcgggctcg ccgaggtctg tttccaaagt cgcccttgat
       61 ggcggcggag gcaaggcggc cgcggcgcgg agcagccgac gcacgctagt gggtccgccc
      121 gccaccgccc cttcctcggc gtccgctccc gcccttgccg tcccccgcgc ggctccgcgc
      181 ctcgggcccc gggcgcagcc agccctccag acgcccgcgg tcccggcggc gtgtgctgct
      241 cttcctccgc ccgcggtttc cagcgccgct ccttcccccg cttgggcagg gagggggcat
      301 tgatcttcga tcgcgaagat ggctgctggc tgcctgctgg ccttgactct gacacttttc
      361 caatctttgc tcatcggccc ctcgtcggag gagccgttcc cttcggccgt cactatcaaa
      421 tcatgggtgg ataagatgca agaagacctt gtcacactgg caaaaacagc aagtggagtc
      481 aatcagcttg ttgatattta tgagaaatat caagatttgt atactgtgga accaaataat
      541 gcacgccagc tggtagaaat tgcagccagg gatattgaga aacttctgag caacagatct
      601 aaagccctgg tgcgcctggc attggaagcg gagaaagttc aagcagctca ccagtggaga
      661 gaagattttg caagcaatga agttgtctac tacaatgcaa aggatgatct cgatcctgag
      721 aaaaatgaca gtgagccagg cagccagagg ataaaacctg ttttcattga agatgctaat
      781 tttggacgac aaatatctta tcagcacgca gcagtccata ttcctactga catctatgag
      841 ggctcaacaa ttgtgttaaa tgaactcaac tggacaagtg ccttagatga agttttcaaa
      901 aagaatcgcg aggaagaccc ttcattattg tggcaggttt ttggcagtgc cactggccta
      961 gctcgatatt atccagcttc accatgggtt gataatagta gaactccaaa taagattgac
     1021 ctttatgatg tacgcagaag accatggtac atccaaggag ctgcatctcc taaagacatg
     1081 cttattctgg tggatgtgag tggaagtgtt agtggattga cacttaaact gatccgaaca
     1141 tctgtctccg aaatgttaga aaccctctca gatgatgatt tcgtgaatgt agcttcattt
     1201 aacagcaatg ctcaggatgt aagctgtttt cagcaccttg tccaagcaaa tgtaagaaat
     1261 aaaaaagtgt tgaaagacgc ggtgaataat atcacagcca aaggaattac agattataag
     1321 aagggcttta gttttgcttt tgaacagctg cttaattata atgtttccag agcaaactgc
     1381 aataagatta ttatgctatt cacggatgga ggagaagaga gagcccagga gatatttaac
     1441 aaatacaata aagataaaaa agtacgtgta ttcacgtttt cagttggtca acacaattat
     1501 gacagaggac ctattcagtg gatggcctgt gaaaacaaag gttattatta tgaaattcct
     1561 tccattggtg caataagaat caatactcag gaatatttgg atgttttggg aagaccaatg
     1621 gttttagcag gagacaaagc taagcaagtc caatggacaa atgtgtacct ggatgcattg
     1681 gaactgggac ttgtcattac tggaactctt ccggtcttca acataaccgg ccaatttgaa
     1741 aataagacaa acttaaagaa ccagctgatt cttggtgtga tgggagtaga tgtgtctttg
     1801 gaagatatta aaagactgac accacgtttt acactgtgcc ccaatgggta ttactttgca
     1861 atcgatccta atggttatgt tttattacat ccaaatcttc agccaaagaa ccccaaatct
     1921 caggagccag taacattgga tttccttgat gcagagttag agaatgatat taaagtggag
     1981 attcgaaata agatgattga tggggaaagt ggagaaaaaa cattcagaac tctggttaaa
     2041 tctcaagatg agagatatat tgacaaagga aacaggacat acacatggac acctgtcaat
     2101 ggcacagatt acagtttggc cttggtatta ccaacctaca gtttttacta tataaaagcc
     2161 aaactagaag agacaataac tcaggccaga tcaaaaaagg gcaaaatgaa ggattcggaa
     2221 accctgaagc cagataattt tgaagaatct ggctatacat tcatagcacc aagagattac
     2281 tgcaatgacc tgaaaatatc ggataataac actgaatttc ttttaaattt caacgagttt
     2341 attgatagaa aaactccaaa caacccatca tgtaacgcgg atttgattaa tagagtcttg
     2401 cttgatgcag gctttacaaa tgaacttgtc caaaattact ggagtaagca gaaaaatatc
     2461 aagggagtga aagcacgatt tgttgtgact gatggtggga ttaccagagt ttatcccaaa
     2521 gaggctggag aaaattggca agaaaaccca gagacatatg aggacagctt ctataaaagg
     2581 agcctagata atgataacta tgttttcact gctccctact ttaacaaaag tggacctggt
     2641 gcctatgaat cgggcattat ggtaagcaaa gctgtagaaa tatatattca agggaaactt
     2701 cttaaacctg cagttgttgg aattaaaatt gatgtaaatt cctggataga gaatttcacc
     2761 aaaacctcaa tcagagatcc gtgtgctggt ccagtttgtg actgcaaaag aaacagtgac
     2821 gtaatggatt gtgtgattct ggatgatggt gggtttcttc tgatggcaaa tcatgatgat
     2881 tatactaatc agattggaag attttttgga gagattgatc ccagcttgat gagacacctg
     2941 gttaatatat cagtttatgc ttttaacaaa tcttatgatt atcagtcagt atgtgagccc
     3001 ggtgctgcac caaaacaagg agcaggacat cgctcagcat atgtgccatc agtagcagac
     3061 atattacaaa ttggctggtg ggccactgct gctgcctggt ctattctaca gcagtttctc
     3121 ttgagtttga cctttccacg actccttgag gcagttgaga tggaggatga tgacttcacg
     3181 gcctccctgt ccaagcagag ctgcattact gaacaaaccc agtatttctt cgataacgac
     3241 agtaaatcat tcagtggtgt attagactgt ggaaactgtt ccagaatctt tcatggagaa
     3301 aagcttatga acaccaactt aatattcata atggttgaga gcaaagggac atgtccatgt
     3361 gacacacgac tgctcataca agcggagcag acttctgacg gtccaaatcc ttgtgacatg
     3421 gttaagcaac ccagataccg aaaagggcct gatgtctgct ttgataacaa tgtcttggag
     3481 gattatactg actgtggtgg tgtttctgga ttaaatccct ccctgtggta tatcattgga
     3541 atccagtttc tactactttg gctggtatct ggcagcacac accgcctgtt atgaccttct
     3601 aaaaaccaaa tctgcatagt taaactccag accctgccaa aacatgagcc ctgccctcaa
     3661 ttacagtaac gtagggtcag ctataaaatc agacaaacat tagctgggcc tgttccatgg
     3721 cataacacta aggcgcagac tcctaaggca cccactggct gcatgtcagg gtgtcagatc
     3781 cttaaacgtg tgtgaatgct gcatcatcta tgtgtaacat caaagcaaaa tcctatacgt
     3841 gtcctctatt ggaaaatttg ggagtttgtt gttgcattgt tggtgattac atgtgaaagg
     3901 gttccccata caattgttaa tgaaccataa gaaatgtctt gatattgacc tggaattttg
     3961 acttgctgca attttactaa gaaaatctct aaggggaaag aaattatttt tgccttcact
     4021 ttttcttctg tgaaaagtta attcctgctt taagtagcaa ttattgaaat atatagagaa
     4081 agagagaatt aacattggtc taaattgttg aaatataaat aatggctaat tttctatgaa
     4141 aaatttgcca tgaataaaat gccttatgaa gaacggcttc tttgccaaaa ataaaacaca
     4201 attgatgacc aattcaattt tgatggggta atgaattgat ggtttgaaaa tagtaactaa
     4261 gaaattatta aactaaaggt gcttgaggga gaaattagaa tatgtgacat atttcctcat
     4321 aaatgtaagc aagcccttaa taaatgcttt gatgtaatgt tccttttgtt cacagtacag
     4381 ttatgtagtg ctagccagat atttaaaaat gctctaagaa aagcttgtca ggggggaggg
     4441 ggaaatgttt ttcttgtgat aaatgaactt tggtttacca agggtatttt gcatgatgct
     4501 gctgctattt taacttttca gttgtttttt ctcttactgt agagtgtagt cattgaaaag
     4561 ttaactgagt gatattgtta tgtaagaatc tgaaccttgc agtgtaaatg cacttaagtc
     4621 atctttaaaa tgttgttaaa ctactctgaa tatactgtgc aatgtaaatg ctgaatgatt
     4681 ggggttagat aaatggtgaa atgagaatta atgaatttta tacagattca tgctttcgcc
     4741 tgataaccta tgtacagata ttttaagtac ataaatcaga tttgacacat ttattttttg
     4801 aaaagtacat atatgttctc aatgaagatt catcctaggt ttcctctttg ttcgtttcaa
     4861 tagtagcaca tacaatccaa aagttcctgc tactgctgct tttgcccttc cattttaaat
     4921 ggtatgatcg tttggccaaa atttccatgc aagttgataa gggttttaca ataatctgaa
     4981 caaataggaa accttgaatc tctatgtatg cacatgaata cttgattgtg aataataaaa
     5041 gttgtttttt atacagcctc attggtgaaa atggttagtg tagtaaatca gatatttcat
     5101 tatagactaa tatggtagca gattatttat gcttttaaaa attattttct ggagcaaaca
     5161 gtatttgaag aaaaggtaaa aactatgaat gtgtaattct aagaatagtt aataatttta
     5221 ctgaaggctg aacgtatgta gatatttttt atgataaaat gcagtttttc tttttttgga
     5281 acatgccaat tactggtatg tgtatatgct ggaaattttc ttacatatcc tttttaagtc
     5341 aaagttatta acattatcca gcatgcttta atatgctcca catatcaaag atataaccaa
     5401 taatttaacg tagcttaaaa gataacattt gttttgtttt tagctttgca tacatttgta
     5461 attccctccc accaccaata taccttaggt accatttgtg tacatttcaa gatttttttt
     5521 ttcaataaaa aggtgggggg aaatggaatc actttgccaa ctattgttct aagagttgac
     5581 atcagttacc atttctaatg cattgttgtg attttgtagt gtcattcata actggtattg
     5641 acattttaga aaacaccaaa gtgatttaaa atatatgtgg ggaaaaaaac ttctcactct
     5701 agtaacaatg ttttaatcat gttgtttgtt aaatatttaa aagttattgt tcaatttttg
     5761 ctagactatc ttaaatcatg atagtgttgg gaagggggta tcaaaatata aaattgttag
     5821 tttgtgtata taaacaatgc acattaaaac aattgaattg cttccagcat acaacaaact
     5881 accttaaaat tatgttcaaa atctattaaa tatattttca tggctaatgt cgagtatatg
     5941 ttttcatcaa ttacattgtt attgtattaa tcgcggttaa acttggcatc tcttggcagg
     6001 atcctattta atttctgttg ccatcaaaat atatttatat gcatgagttt tgctataaac
     6061 ctttattttc tgctataaag ttatatatta tgacaaccat aaatgcaatt acaaattggg
     6121 taagaacaag ctgaataaaa gttgttccca tgactaatat tagtcttcat gcttttagca
     6181 tattttttaa gacagtaaca ctcatcccat caaattatta cattacattt agtatttttc
     6241 tgagttacat tgacaagaac ttaaattata gcctgttatt aatattgatt tccaacgact
     6301 gaacagtaca acagatcaat aaaaaagttt atacactgct ttaactaatt tttgttatac
     6361 tcatgtggct tagtgtagtt agtcactaaa acagagttca agtgagcttg gcaatggtgt
     6421 tgagtaacaa gaaagaattt tgcattaatg atttcttttt attttcctca tttaaacaac
     6481 cagcattact gccaaacacc aaccgtggtc catatttgta acaggttttt aacatgacat
     6541 agttagcaag tttgattgaa gagattttta ggtcctgggc ctataagatt ttagtatgat
     6601 cttttttcat gtttctaatg cttgaggtgt tactgcttaa cttgggaaaa aaaaaaacaa
     6661 aaaacagtgt tcctgccatt tgtaagtttt cctataatat tccttttatt aactttaaaa
     6721 ctggacttat cgggttcaaa taggcagtat cccttttaag tgacctttgc aacccaagtg
     6781 ttacttgctt atttgatgct ctcttgtatt ttaaatctca tttaaatctt tgtctgtgac
     6841 caacaaagct taagattttg tcttcattta aaactctgaa tctattccat tgatcatgtt
     6901 agacgttcaa atgtttaatg gtatagtctg acctagtgaa acaaaatgga tgttgttact
     6961 ttttttaaag aatagaatga aataactatt tcacttgccc aaaattttaa atattttttc
     7021 atctaaactt gtattgtttc ccatagcaga atgtcaatat tcacagtaca tttctgtaaa
     7081 gagcaaacca atataatgtt ttgagtgttg aaaaaaattc cagatttttg aagaattaga
     7141 caactcttca tctaccttat ttctagttca cacagttatc tcaaattcca ctgaaactaa
     7201 tgggatactg tcttgtgtag atgccagttg agtttataat gtgacctagt aaagctgtct
     7261 tttttgttgt gttgtatgag tgtcggatca tgcttttagg aatactttta ttaaaatggt
     7321 gtgcattcat gcaaaaggcc aactggcttt tgtgaacaat agatcttttc tcccctttat
     7381 tttgttctct tgacactttt gtgaaaatta cctagcctga taaaaacatt ttgtaaaaat
     7441 ttgtataata ctgttataaa atatttataa tcccagaaaa tgttgtgtta aatcttgtgc
     7501 aaaaacagta tattcagaat aaaagtttat catttaaagt ca
//
