LOCUS       NM_021096              10002 bp    mRNA    linear   PRI 01-JUL-2024
DEFINITION  Homo sapiens calcium voltage-gated channel subunit alpha1 I
            (CACNA1I), transcript variant 1, mRNA.
ACCESSION   NM_021096
VERSION     NM_021096.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Jeong,S., Lee,B.Y., Rhee,J.S. and Lee,J.H.
  TITLE     G protein beta subunits regulate Cav3.3 T-type channel activity and
            current kinetics via interaction with the Cav3.3 C-terminus
  JOURNAL   Biochim Biophys Acta Biomembr 1866 (6), 184337 (2024)
   PUBMED   38763272
  REMARK    GeneRIF: G protein beta subunits regulate Cav3.3 T-type channel
            activity and current kinetics via interaction with the Cav3.3
            C-terminus.
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Maksemous,N., Harder,A.V.E., Ibrahim,O., Vijfhuizen,L.S.,
            Sutherland,H., Pelzer,N., de Boer,I., Terwindt,G.M., Lea,R.A., van
            den Maagdenberg,A.M.J.M. and Griffiths,L.R.
  TITLE     Whole Exome Sequencing of Hemiplegic Migraine Patients Shows an
            Increased Burden of Missense Variants in CACNA1H and CACNA1I Genes
  JOURNAL   Mol Neurobiol 60 (6), 3034-3043 (2023)
   PUBMED   36786913
  REMARK    GeneRIF: Whole Exome Sequencing of Hemiplegic Migraine Patients
            Shows an Increased Burden of Missense Variants in CACNA1H and
            CACNA1I Genes.
REFERENCE   3  (bases 1 to 10002)
  AUTHORS   Baez-Nieto,D., Allen,A., Akers-Campbell,S., Yang,L., Budnik,N.,
            Pupo,A., Shin,Y.C., Genovese,G., Liao,M., Perez-Palma,E., Heyne,H.,
            Lal,D., Lipscombe,D. and Pan,J.Q.
  TITLE     Analysing an allelic series of rare missense variants of CACNA1I in
            a Swedish schizophrenia cohort
  JOURNAL   Brain 145 (5), 1839-1853 (2022)
   PUBMED   34919654
  REMARK    GeneRIF: Analysing an allelic series of rare missense variants of
            CACNA1I in a Swedish schizophrenia cohort.
REFERENCE   4  (bases 1 to 10002)
  AUTHORS   El Ghaleb,Y., Schneeberger,P.E., Fernandez-Quintero,M.L.,
            Geisler,S.M., Pelizzari,S., Polstra,A.M., van Hagen,J.M.,
            Denecke,J., Campiglio,M., Liedl,K.R., Stevens,C.A., Person,R.E.,
            Rentas,S., Marsh,E.D., Conlin,L.K., Tuluc,P., Kutsche,K. and
            Flucher,B.E.
  TITLE     CACNA1I gain-of-function mutations differentially affect channel
            gating and cause neurodevelopmental disorders
  JOURNAL   Brain 144 (7), 2092-2106 (2021)
   PUBMED   33704440
REFERENCE   5  (bases 1 to 10002)
  AUTHORS   Zhang,D., Chen,J., Saraf,A., Cassar,S., Han,P., Rogers,J.C.,
            Brioni,J.D., Sullivan,J.P. and Gopalakrishnan,M.
  TITLE     Association of Catsper1 or -2 with Ca(v)3.3 leads to suppression of
            T-type calcium channel activity
  JOURNAL   J Biol Chem 281 (31), 22332-22341 (2006)
   PUBMED   16740636
  REMARK    GeneRIF: CatSper1 and CatSper2 can associate with and modulate the
            function of the Ca(v)3.3 channel, which might be important in the
            regulation of sperm function.
REFERENCE   6  (bases 1 to 10002)
  AUTHORS   Murbartian,J., Arias,J.M. and Perez-Reyes,E.
  TITLE     Functional impact of alternative splicing of human T-type Cav3.3
            calcium channels
  JOURNAL   J Neurophysiol 92 (6), 3399-3407 (2004)
   PUBMED   15254077
  REMARK    GeneRIF: intracellular regions after repeats I and IV play a role
            in gating interdependently, suggesting a direct interaction, and
            splice variation of Cav3.3 channels provides a mechanism for
            fine-tuning latency and duration of low-threshold spikes.
REFERENCE   7  (bases 1 to 10002)
  AUTHORS   Gomora,J.C., Murbartian,J., Arias,J.M., Lee,J.H. and Perez-Reyes,E.
  TITLE     Cloning and expression of the human T-type channel Ca(v)3.3:
            insights into prepulse facilitation
  JOURNAL   Biophys J 83 (1), 229-241 (2002)
   PUBMED   12080115
REFERENCE   8  (bases 1 to 10002)
  AUTHORS   Chemin,J., Monteil,A., Perez-Reyes,E., Bourinet,E., Nargeot,J. and
            Lory,P.
  TITLE     Specific contribution of human T-type calcium channel isotypes
            (alpha(1G), alpha(1H) and alpha(1I)) to neuronal excitability
  JOURNAL   J Physiol 540 (Pt 1), 3-14 (2002)
   PUBMED   11927664
  REMARK    GeneRIF: Specific contribution of human T-type calcium channel
            isotypes (alpha(1G), alpha(1H) and alpha(1I)) to neuronal
            excitability.
REFERENCE   9  (bases 1 to 10002)
  AUTHORS   Monteil,A., Chemin,J., Leuranguer,V., Altier,C., Mennessier,G.,
            Bourinet,E., Lory,P. and Nargeot,J.
  TITLE     Specific properties of T-type calcium channels generated by the
            human alpha 1I subunit
  JOURNAL   J Biol Chem 275 (22), 16530-16535 (2000)
   PUBMED   10749850
REFERENCE   10 (bases 1 to 10002)
  AUTHORS   Mittman,S., Guo,J., Emerick,M.C. and Agnew,W.S.
  TITLE     Structure and alternative splicing of the gene encoding alpha1I, a
            human brain T calcium channel alpha1 subunit
  JOURNAL   Neurosci Lett 269 (3), 121-124 (1999)
   PUBMED   10454147
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF142567.1, AF211189.1,
            AF393329.1 and N52765.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_021096.3.
            
            Summary: This gene encodes the pore-forming alpha subunit of a
            voltage gated calcium channel. The encoded protein is a member of a
            subfamily of calcium channels referred to as is a low
            voltage-activated, T-type, calcium channel. The channel encoded by
            this protein is characterized by a slower activation and
            inactivation compared to other T-type calcium channels. This
            protein may be involved in calcium signaling in neurons. Alternate
            splicing results in multiple transcript variants. [provided by
            RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF142567.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03465403 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000402142.4/ ENSP00000385019.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2156              AF142567.1         1-2156
            2157-5830           AF211189.1         2052-5725
            5831-6990           AF142567.1         5831-6990
            6991-9997           AF393329.1         6890-9896
            9998-10002          N52765.1           6-10                c
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.1"
     gene            1..10002
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /note="calcium voltage-gated channel subunit alpha1 I"
                     /db_xref="GeneID:8911"
                     /db_xref="HGNC:HGNC:1396"
                     /db_xref="MIM:608230"
     CDS             1..6672
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /note="isoform a is encoded by transcript variant 1;
                     calcium channel, voltage-dependent, alpha 1I subunit;
                     voltage-dependent T-type calcium channel subunit alpha-1I;
                     voltage-gated calcium channel subunit alpha Cav3.3;
                     calcium channel, voltage-dependent, T type, alpha 1I
                     subunit"
                     /codon_start=1
                     /product="voltage-dependent T-type calcium channel subunit
                     alpha-1I isoform a"
                     /protein_id="NP_066919.2"
                     /db_xref="CCDS:CCDS46710.1"
                     /db_xref="GeneID:8911"
                     /db_xref="HGNC:HGNC:1396"
                     /db_xref="MIM:608230"
                     /translation="MAESASPPSSSAAAPAAEPGVTTEQPGPRSPPSSPPGLEEPLDG
                     ADPHVPHPDLAPIAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPC
                     DDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMA
                     GMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFF
                     VFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNG
                     IMGCHEIPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPH
                     KGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINL
                     CLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAK
                     RRALGLYQALQSRRQALGPEAPAPAKPGPHAKEPRHYHGKTKGQGDEGRHLGSRHCQT
                     LHGPASPGNDHSGRELCPQHSPLDATPHTLVQPIPATLASDPASCPCCQHEDGRRPSG
                     LGSTDSGQEGSGSGSSAGGEDEADGDGARSSEDGASSELGKEEEEEEQADGAVWLCGD
                     VWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSM
                     FALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLK
                     LVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTV
                     PDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNL
                     LVAILVEGFQAEGDANRSYSDEDQSSSNIEEFDKLQEGLDSSGDPKLCPIPMTPNGHL
                     DPSLPLGGHLGPAGAAGPAPRLSLQPDPMLVALGSRKSSVMSLGRMSYDQRSLSSSRS
                     SYYGPWGRSAAWASRRSSWNSLKHKPPSAEHESLLSAERGGGARVCEVAADEGPPRAA
                     PLHTPHAHHIHHGPHLAHRHRHHRRTLSLDNRDSVDLAELVPAVGAHPRAAWRAAGPA
                     PGHEDCNGRMPSIAKDVFTKMGDRGDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVRE
                     DWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFL
                     TVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSLASA
                     GGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIF
                     GILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLAS
                     KDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHK
                     CRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCHTRLLIHSMCTSHYLDIFIT
                     FIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWN
                     QLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLD
                     TVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAF
                     LTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVV
                     AVLMKHLDDSNKEAQEDAEMDAELELEMAHGLGPGPRLPTGSPGAPGRGPGGAGGGGD
                     TEGGLCRRCYSPAQENLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCKKCH
                     HDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPTACPPGRKDSKGELDPPEPMRVG
                     DLGECFFPLSSTAVSPDPENFLCEMEEIPFNPVRSWLKHDSSQAPPSPFSPDASSPLL
                     PMPAEFFHPAVSASQKGPEKGTGTGTLPKIALQGSWASLRSPRVNCTLLRQATGSDTS
                     LDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPAPAPGPRAGLSPAARRRLSLRGRGL
                     FSLRGLRAHQRSHSSGGSTSPGCTHHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSL
                     TSLFCPPPPPPAPGLTPARKFSSTSSLAAPGRPHAAALAHGLARSPSWAADRSKDPPG
                     RAPLPMGLGPLAPPPQPLPGELEPGDAASKRKR"
     exon            1..236
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            237..348
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            349..482
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            483..580
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            581..740
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            741..1056
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1149
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1462
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1567
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1992
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2144
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2330
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2448
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2449..2604
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2605..2698
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2699..2901
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            2902..3372
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            3373..3473
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3597
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            3598..3666
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            3667..3851
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            3852..3978
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            3979..4104
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            4105..4194
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            4195..4387
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            4388..4539
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            4540..4649
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            4650..4783
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            4784..4854
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            4855..4933
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            4934..5055
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            5056..5394
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            5395..5541
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            5542..5664
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            5665..5830
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            5831..6027
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     exon            6028..10002
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9975..9980
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /note="hexamer: AATAAA"
     polyA_site      10002
                     /gene="CACNA1I"
                     /gene_synonym="ca(v)3.3; Cav3.3; NEDSIS"
                     /note="major polyA site"
ORIGIN      
        1 atggctgaga gcgcctcccc gccctcctca tctgcagcag ccccagccgc tgagccagga
       61 gtcaccacgg agcagcccgg accccggagc cccccatcct ccccgccagg cctggaggag
      121 cctctggatg gagctgatcc tcatgtccca cacccagacc tggcgcctat tgccttcttc
      181 tgcctgcgac agaccaccag cccccggaac tggtgcatca agatggtgtg caacccgtgg
      241 tttgaatgtg tcagcatgct ggtgatcctg ctgaactgcg tgacacttgg catgtaccag
      301 ccgtgcgacg acatggactg cctgtccgac cgctgcaaga tcctgcaggt ctttgatgac
      361 ttcatcttta tcttctttgc catggagatg gtgctcaaga tggtggccct ggggattttt
      421 ggcaagaagt gctacctcgg ggacacatgg aaccgcctgg atttcttcat cgtcatggca
      481 gggatggtcg agtactccct ggaccttcag aacatcaacc tgtcagccat ccgcaccgtg
      541 cgcgtcctga ggcccctcaa agccatcaac cgcgtgccca gtatgcggat cctggtgaac
      601 ctgctcctgg acacactgcc catgctgggg aatgtcctgc tgctctgctt ctttgtcttc
      661 ttcatctttg gcatcatagg tgtgcagctc tgggcgggcc tgctgcgtaa ccgctgcttc
      721 ctggaggaga acttcaccat acaaggggat gtggccttgc ccccatacta ccagccggag
      781 gaggatgatg agatgccctt catctgctcc ctgtcgggcg acaatgggat aatgggctgc
      841 catgagatcc ccccgctcaa ggagcagggc cgtgagtgct gcctgtccaa ggacgacgtc
      901 tacgactttg gggcggggcg ccaggacctc aatgccagcg gcctctgtgt caactggaac
      961 cgttactaca atgtgtgccg cacgggcagc gccaaccccc acaagggtgc catcaacttt
     1021 gacaacatcg gttatgcttg gattgtcatc ttccaggtga tcactctgga aggctgggtg
     1081 gagatcatgt actacgtgat ggatgctcac tccttctaca acttcatcta cttcatcctg
     1141 cttatcatag tgggctcctt cttcatgatc aacctgtgcc tcgttgtcat agcgacccag
     1201 ttctcggaga ccaagcaacg ggagcaccgg ctgatgctgg agcagcggca gcgctacctg
     1261 tcctccagca cggtggccag ctacgccgag cctggcgact gctacgagga gatcttccag
     1321 tatgtctgcc acatcctgcg caaggccaag cgccgcgccc tgggcctcta ccaggccctg
     1381 cagagccggc gccaggccct gggcccggag gccccggccc ccgccaaacc tgggccccac
     1441 gccaaggagc cccggcacta ccatgggaag actaagggtc agggagatga agggagacat
     1501 ctcggaagcc ggcattgcca gactttgcat gggcctgcct cccctggaaa tgatcactcg
     1561 ggaagagagc tgtgcccgca acatagcccc ctggatgcga cgccccacac cctggtgcag
     1621 cccatccccg ccacgctggc ttccgatccc gccagctgcc cttgctgcca gcatgaggac
     1681 ggccggcggc cctcgggcct gggcagcacc gactcgggcc aggagggctc gggctccggg
     1741 agctccgctg gtggcgagga cgaggcggat ggggacgggg cccggagcag cgaggacgga
     1801 gcctcctcag aactggggaa ggaggaggag gaggaggagc aggcggatgg ggcggtctgg
     1861 ctgtgcgggg atgtgtggcg ggagacgcga gccaagctgc gcggcatcgt ggacagcaag
     1921 tacttcaacc ggggcatcat gatggccatc ctggtcaaca ccgtcagcat gggcatcgag
     1981 caccacgagc agccggagga gctgaccaac atcctggaga tctgcaatgt ggtcttcacc
     2041 agcatgtttg ccctggagat gatcctgaag ctggctgcat ttgggctctt cgactacctg
     2101 cgtaacccct acaacatctt cgacagcatc attgtcatca tcagcatctg ggagattgtg
     2161 gggcaggcgg acggtgggct gtcggtgctg cggaccttcc ggctgctgcg cgtgctgaaa
     2221 ctggtgcgct tcatgcctgc cctgcggcgc cagctcgtgg tgctcatgaa gaccatggac
     2281 aacgtggcca ccttctgcat gctgctcatg ctcttcatct tcatcttcag catccttggg
     2341 atgcatattt ttggctgcaa gttcagcctc cgcacggaca ctggagacac ggtgcccgac
     2401 aggaagaact tcgactccct gctgtgggcc atcgtcactg tgttccagat cctcacccag
     2461 gaggactgga acgtcgttct ctacaatggc atggcctcca cttctccctg ggcctccctc
     2521 tactttgtcg ccctcatgac cttcggcaac tatgtgctct tcaacctgct ggtggccatc
     2581 ctggtggagg gcttccaggc ggagggtgac gccaatcgct cctactcgga cgaggaccag
     2641 agctcatcca acatagaaga gtttgataag ctccaggaag gcctggacag cagcggagat
     2701 cccaagctct gcccaatccc catgaccccc aatgggcacc tggaccccag tctcccactg
     2761 ggtgggcacc taggtcctgc tggggctgcg ggacctgccc cccgactctc actgcagccg
     2821 gaccccatgc tggtggccct gggctcccga aagagcagtg tcatgtctct agggaggatg
     2881 agctatgacc agcgctccct gtccagctcc cggagctcct actacgggcc atggggccgc
     2941 agcgcggcct gggccagccg tcgctccagc tggaacagcc tcaagcacaa gccgccgtcg
     3001 gcggagcatg agtccctgct ctctgcggag cgcggcggcg gcgcccgggt ctgcgaggtt
     3061 gccgcggacg aggggccgcc gcgggccgca cccctgcaca ccccacacgc ccaccacatt
     3121 catcacgggc cccatctggc gcaccgccac cgccaccacc gccggacgct gtccctcgac
     3181 aacagggact cggtggacct ggccgagctg gtgcccgcgg tgggcgccca cccccgggcc
     3241 gcctggaggg cggcaggccc ggcccccggg catgaggact gcaatggcag gatgcccagc
     3301 atcgccaaag acgtcttcac caagatgggc gaccgcgggg atcgcgggga ggatgaggag
     3361 gaaatcgact acaccctgtg cttccgcgtc cgcaagatga tcgacgtcta taagcccgac
     3421 tggtgcgagg tccgcgaaga ctggtctgtc tacctcttct ctcccgagaa caggttccgg
     3481 gtcctgtgtc agaccattat tgcccacaaa ctcttcgact acgtcgtcct ggccttcatc
     3541 tttctcaact gcatcaccat cgccctggag cggcctcaga tcgaggccgg cagcaccgaa
     3601 cgcatctttc tcaccgtgtc caactacatc ttcacggcca tcttcgtggg cgagatgaca
     3661 ttgaaggtag tctcgctggg cctgtacttc ggcgagcagg cgtacctacg cagcagctgg
     3721 aacgtgctgg atggctttct tgtcttcgtg tccatcatcg acatcgtggt gtccctggcc
     3781 tcagccgggg gagccaagat cttgggggtc ctccgagtct tgcggctcct gcgcacccta
     3841 cgccccctgc gtgtcatcag ccgggcgccg ggcctgaagc tggtggtgga gacactcatc
     3901 tcctccctca agcccatcgg caacatcgtg ctcatctgct gtgccttctt catcatcttt
     3961 ggcatcctgg gagtgcagct cttcaagggc aagttctacc actgtctggg cgtggacacc
     4021 cgcaacatca ccaaccgctc ggactgcatg gccgccaact accgctgggt ccatcacaaa
     4081 tacaacttcg acaacctggg ccaggctctg atgtccctct ttgtcctggc atccaaggat
     4141 ggttgggtga acatcatgta caatggactg gatgctgttg ctgtggacca gcagcctgtg
     4201 accaaccaca acccctggat gctgctgtac ttcatctcct tcctgctcat cgtcagcttc
     4261 tttgtgctca acatgtttgt gggtgtcgtg gtggagaact tccacaagtg ccggcagcac
     4321 caggaggctg aagaggcacg gcggcgtgag gagaagcggc tgcggcgcct ggagaagaag
     4381 cgccggaagg cccagcggct gccctactat gccacctatt gtcacacccg gctgctcatc
     4441 cactccatgt gcaccagcca ctacctggac atcttcatca ccttcatcat ctgcctcaac
     4501 gtggtcacca tgtccctgga gcactacaat cagcccacgt ccctggagac agccctcaag
     4561 tactgcaact atatgttcac cactgtcttt gtgctggagg ctgtgctgaa gctggtggca
     4621 tttggtctga ggcgcttctt caaggaccga tggaaccagc tggacctggc cattgtgcta
     4681 ctgtcagtca tgggcatcac cctggaggag atcgagatca atgcggccct gcccatcaat
     4741 cccaccatca tccgcatcat gagggttctg cgcattgccc gagtgctgaa gctgttgaag
     4801 atggccacag gaatgcgggc cctgctggac acggtggtgc aagctttgcc ccaggtgggc
     4861 aacctgggcc tcctcttcat gctgctcttc ttcatctatg ctgctctcgg ggtggagctc
     4921 tttgggaagc tggtctgcaa cgacgagaac ccgtgcgagg gcatgagccg gcatgccacc
     4981 ttcgagaact tcggcatggc cttcctcaca ctcttccagg tctccacggg tgacaactgg
     5041 aacgggatca tgaaggacac gctgcgggac tgcacccacg acgagcgcag ctgcctgagc
     5101 agcctgcagt ttgtgtcgcc gctgtacttc gtgagcttcg tgctcaccgc gcagttcgtg
     5161 ctcatcaacg tggtggtggc tgtgctcatg aagcacctgg acgacagcaa caaggaggcg
     5221 caggaggacg ccgagatgga tgccgagctc gagctggaga tggcccatgg cctgggccct
     5281 ggcccgaggc tgcctaccgg ctccccgggc gcccctggcc gagggccggg aggggcgggc
     5341 ggcgggggcg acaccgaggg cggcttgtgc cggcgctgct actcgcctgc ccaggagaac
     5401 ctgtggctgg acagcgtctc tttaatcatc aaggactcct tggaggggga gctgaccatc
     5461 atcgacaacc tgtcgggctc catcttccac cactactcct cgcctgccgg ctgcaagaag
     5521 tgtcaccacg acaagcaaga ggtgcagctg gctgagacgg aggccttctc cctgaactca
     5581 gacaggtcct cgtccatcct gctgggtgac gacctgagtc tcgaggaccc cacagcctgc
     5641 ccacctggcc gcaaagacag caagggtgag ctggacccac ctgagcccat gcgtgtggga
     5701 gacctgggcg aatgcttctt ccccttgtcc tctacggccg tctcgccgga tccagagaac
     5761 ttcctgtgtg agatggagga gatcccattc aaccctgtcc ggtcctggct gaaacatgac
     5821 agcagtcaag cacccccaag tcccttctcc ccggatgcct ccagccctct cctgcccatg
     5881 ccagccgagt tcttccaccc tgcagtgtct gccagccaga aaggcccaga aaagggcact
     5941 ggcactggaa ccctccccaa gattgcgctg cagggctcct gggcatctct gcggtcacca
     6001 agggtcaact gtaccctcct ccggcaggcc accgggagcg acacgtcgct ggacgccagc
     6061 cccagcagct ccgcgggcag cctgcagacc acgctcgagg acagcctgac cctgagcgac
     6121 agcccccggc gtgccctggg gccgcccgcg cctgctccag gaccccgggc cggcctgtcc
     6181 cccgccgctc gccgccgcct gagcctgcgc ggccggggcc tcttcagcct gcgggggctg
     6241 cgggcgcatc agcgcagcca cagcagcggg ggctccacca gcccgggctg cacccaccac
     6301 gactccatgg acccctcgga cgaggagggc cgcggtggcg cgggcggcgg gggcgcgggc
     6361 agcgagcact cggagaccct cagcagcctc tcgctcacct ccctcttctg cccgccgccc
     6421 ccgccgccag cccccggcct cacgcccgcc aggaagttca gcagcaccag cagcctggcc
     6481 gcccccggcc gcccccacgc cgccgccctg gcccacggcc tggcccggag cccctcgtgg
     6541 gccgcggacc gcagcaagga cccccccggc cgggcaccgc tgcccatggg cctgggcccc
     6601 ttggcgcccc cgccgcaacc gctccccgga gagctggagc cgggagacgc cgccagcaag
     6661 aggaagagat gagggtcgca ggggcccccg gccgcccacc gcccgccccg tctcaccttc
     6721 tttacctcag gagccaggag cagacagcaa tacttcgtcc acacctggga tcgcgcaggg
     6781 cccgcagggc acaggcgccc gacagccggg ctgagcggag tctgggttag ccaggcctgc
     6841 gtggcccatg gtggcccttc cagtgcatat acatacatat atatatatat atgcatatat
     6901 atatatatat atatatatat gtgtatacac acacacatag acagacatat atatatatat
     6961 ttattttttt tactgagagc ttatgacttc cagaaagtgc taaaggtggg aggtgggcca
     7021 gggcctggac ggggcttttg tctgatgctc tgggattctg gccagaccca ccccagggca
     7081 catgtcctgc gggggcgtcc cagctgtgtt ttttgatgtc tcctccctgg ttaagagtag
     7141 cttggagagg accctcaggc ctctgagggc accaggccct ggagaagagg tgcaattcag
     7201 ggtgtgtgtg tgtttttttt cctttaaaga agaaacgctg ctaagatccc acgtggctcc
     7261 cacgtgtcgg ggtgtctgtc ctgtcatcct gactgtctcg ttattgtgaa gtctttcgta
     7321 gacaccccag agcacacaca tccccttagt ccaccggtta gatgtctctc tttagaaaaa
     7381 tcaggggtga gtagctgtgt ttccttaggc ctggggtagg ggatggagag cagctccaag
     7441 gctgagctgg ggctctggcc ccaggtgagg tccccagctc ctgagcactt ctgagggggt
     7501 gggttccacc cccaggaggg cgggggtggg tggagcagga gtggaggcag cctgcagagg
     7561 aagggtgcgg gagactgagc cggcagtggc ttgcttggag gggctagggt acccgcctgg
     7621 tctcggctgg ctccagctgc cctgcaggtg cccctgggct caggtagtta gttgttcagc
     7681 cactaatgcc ttttatcccc catatgggcg ctgagctgtg gctgttcctg gacactgtgc
     7741 tgtccccgtc ctgagcaact atgcccccgc cccagtaggt tcaaggcaaa gcagctctga
     7801 ccgaattcta ggcaggggtg ggggcacctg cctgggccct gggtccagcc gcatccccat
     7861 gcccagcctt tcgcgtcacc tggaggccac atcttcccac cccacctgcc agcccctgtc
     7921 tctcctcccc gctgccccta actgcagctc agctttcact atagcagtgc ttcccaaacg
     7981 ggttctagac ttgggtgttt gatttgaaga gttacgcatt tatttcacta tttattggga
     8041 aaaaaatgca ttgaacccgt gatttcacag acattttgct taggtcaagg ctacttttta
     8101 aaaagggagt gactttcatg aagtcagttt gaagaaggag gttgggagca tgttggtggc
     8161 agtgatctgg agaggctgca ggggccacga gtttgcagat gctgtacttc agcaataacc
     8221 ttgcctttgc taagaccccc tccgagggct tcaggggggc ctgccaaggg ggggccttgt
     8281 tcttgttccc aaactctgac ttggaagaac tcagcttgtg gccgggctgg tgagggtgtg
     8341 gggtgcgccc ttcccttttg ggctgaggga gagggggccg ggaggggttg tcaagcccac
     8401 ccgggaaact gagccctgga gaggggaagc agccagccca ggatgcttca gggccctggc
     8461 agcaaagaag ctggggtggt gtggagggcc ctactccaca ccctagagct gtgatgctga
     8521 gcaaggtgcc tgtggcggaa ggaactgccc agtcctccct gggggcaggt agacctgtgg
     8581 ggatggctcg gtccaagccc aacactgccc gctccagaga gcctcagccc tgcctccctt
     8641 tccacagagg tggttttatg tggaacgagg taccttgtct ggggaagaag tggagctggg
     8701 caggcttgac tgctcctggc tgaccctgag ctctcgcagt tggagggaag cagacagact
     8761 tgactttttg caagtccgcg tgttgctgga gggacacgtg tattggagcc cagaagaggg
     8821 gctgtgtggc ctgggaatac catgtggaag gttgggcttc aagttaacag gatcagacac
     8881 cagtcaagca gcctcaggtc atcttgcgac tccactgagc agcctttcct gttgtcacat
     8941 gggttcagag cagctgagca gggaacacgt cctaggcagg tctgacgtga aacctcccct
     9001 taccctgaac cgtgtgactc tgcaagcaga cacctcgagg caggtgttct aggcaaaggg
     9061 atgggcatga gcgaaggctc cagggtccac agagcaggga gtgtggagaa ggtggaagag
     9121 ctgagctcag aggcagagaa gctccttgca gggagcactg ggagatggcc tggcccgtcc
     9181 caggctcctg gaggccccac ctgccgatcc caggcacttg accttgttcc tgtgggtgac
     9241 agagatgaga accagggaag ctgtgatcca ctggccagga caagtcaaca tgggtgaggg
     9301 ttgggcaaag ggcccttcct tcctccccaa ccctgtggtc tggggccatg gcctcaggct
     9361 tgtgtctatc acaaaagcac aaacttgccc taacccacat gggcctggct gtggggaaag
     9421 tggggaccca ggtccctgag ctgtctgctg ggctccgtag agcggtggtg ggcaggcacc
     9481 ttggcatctg tgcagagacg gcccagtctg gccaaatcct cttcctctcc cctgctctac
     9541 cttctccggc accaggcagc cccttaaagg aggacaaagt gtgtaaagcc cgtctgctgt
     9601 cttcccccaa atcctcagct cagagccttc cgcttccagg gccaacccca gccccgtttg
     9661 ctgcgttgtg taagggcttg gggtcttaga agctgctctt tagcccagat acacatactc
     9721 ttttttgtct ttgtgcaata atcagtgttc ctggcagagc ctgggccaag ctgcagccta
     9781 ctgaggaggc agaggccacc tcctccagaa agccctcggc ctgggccgcc gccgtgactc
     9841 tagcactctc agtcgctgta cagtttctat ggtgtgaatg aacttccctc ctagttgctg
     9901 atggcgctgg tacctgctgg cccatggccc gggtgtagag aaaccaagcg gcagccacca
     9961 ccagtgttgt tttgaataaa agcccagaag cctatttaag aa
//
