LOCUS       NM_153769               1305 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens calcium binding tyrosine phosphorylation regulated
            (CABYR), transcript variant 7, mRNA.
ACCESSION   NM_153769
VERSION     NM_153769.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Yu,X.N., Guo,H.Y., Liu,T.T., Zhang,G.C., Zhu,H.R., Shi,X.,
            Song,G.Q., Jiang,X.M., Huang,X.X., Weng,S.Q., Dong,L., Shen,X.Z.,
            Huang,Y.Q. and Zhu,J.M.
  TITLE     Upregulated calcium-binding tyrosine phosphorylation-regulated
            protein-a/b regulates cell proliferation and apoptosis and predicts
            poor prognosis in hepatocellular carcinoma
  JOURNAL   J Cell Biochem 121 (4), 2938-2949 (2020)
   PUBMED   31692072
  REMARK    GeneRIF: Upregulated calcium-binding tyrosine
            phosphorylation-regulated protein-a/b regulates cell proliferation
            and apoptosis and predicts poor prognosis in hepatocellular
            carcinoma.
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Shen,S., Li,D., Liang,J. and Wang,J.
  TITLE     Testis-specific calcium-binding protein CBP86-IV (CABYR) binds with
            phosphoglycerate kinase 2 in vitro and in vivo experiment
  JOURNAL   Andrologia 51 (7), e13287 (2019)
   PUBMED   30972801
  REMARK    GeneRIF: Study of (Co-IP) and reverse co-immunoprecipitation
            (Co-IP) of CBP86-IV (TSCBP86-IV or CABYR) in human sperm cell
            lysate identified phosphoglycerate kinase 2 (PGK2) as interacting
            partner of CABYR. Protein interactions were further confirmed by
            yeast two-hybrid system.
REFERENCE   3  (bases 1 to 1305)
  AUTHORS   Pelloni,M., Paoli,D., Majoli,M., Pallotti,F., Carlini,T., Lenzi,A.
            and Lombardo,F.
  TITLE     Molecular study of human sperm RNA: Ropporin and CABYR in
            asthenozoospermia
  JOURNAL   J Endocrinol Invest 41 (7), 781-787 (2018)
   PUBMED   29247344
  REMARK    GeneRIF: There is downregulation of both ROPN1 and CABYR in
            asthenozoospermic samples and importantly, a positive correlation
            between the expression of the two genes, suggesting that ROPN1 and
            CABYR co-expression is a prerequisite for normal flagellar function
            and sperm motility.
REFERENCE   4  (bases 1 to 1305)
  AUTHORS   Chen,J., Ding,J., Wang,Z., Zhu,J., Wang,X. and Du,J.
  TITLE     Identification of downstream metastasis-associated target genes
            regulated by LSD1 in colon cancer cells
  JOURNAL   Oncotarget 8 (12), 19609-19630 (2017)
   PUBMED   28121627
  REMARK    GeneRIF: Low CABYR expression is associated with colon cancer.
REFERENCE   5  (bases 1 to 1305)
  AUTHORS   Kim,Y.H., Jha,K.N., Mandal,A., Vanage,G., Farris,E., Snow,P.L.,
            Klotz,K., Naaby-Hansen,S., Flickinger,C.J. and Herr,J.C.
  TITLE     Translation and assembly of CABYR coding region B in fibrous sheath
            and restriction of calcium binding to coding region A
  JOURNAL   Dev Biol 286 (1), 46-56 (2005)
   PUBMED   16139264
  REMARK    GeneRIF: splice variants containing CR-B are expressed during
            spermiogenesis and assemble into the fibrous sheath of the
            principal piece; however, calcium binding occurs only to those
            CABYR isoforms containing CABYR-A
REFERENCE   6  (bases 1 to 1305)
  AUTHORS   Hsu,H.C., Lee,Y.L., Cheng,T.S., Howng,S.L., Chang,L.K., Lu,P.J. and
            Hong,Y.R.
  TITLE     Characterization of two non-testis-specific CABYR variants that
            bind to GSK3beta with a proline-rich extensin-like domain
  JOURNAL   Biochem Biophys Res Commun 329 (3), 1108-1117 (2005)
   PUBMED   15752768
  REMARK    GeneRIF: Northern blot showed that CABYR transcripts were expressed
            more distinctly in the fetal brain than in the adult brain,
            suggesting that this protein may play a role during brain
            development.
REFERENCE   7  (bases 1 to 1305)
  AUTHORS   Ficarro,S., Chertihin,O., Westbrook,V.A., White,F., Jayes,F.,
            Kalab,P., Marto,J.A., Shabanowitz,J., Herr,J.C., Hunt,D.F. and
            Visconti,P.E.
  TITLE     Phosphoproteome analysis of capacitated human sperm. Evidence of
            tyrosine phosphorylation of a kinase-anchoring protein 3 and
            valosin-containing protein/p97 during capacitation
  JOURNAL   J Biol Chem 278 (13), 11579-11589 (2003)
   PUBMED   12509440
REFERENCE   8  (bases 1 to 1305)
  AUTHORS   Naaby-Hansen,S., Mandal,A., Wolkowicz,M.J., Sen,B., Westbrook,V.A.,
            Shetty,J., Coonrod,S.A., Klotz,K.L., Kim,Y.H., Bush,L.A.,
            Flickinger,C.J. and Herr,J.C.
  TITLE     CABYR, a novel calcium-binding tyrosine phosphorylation-regulated
            fibrous sheath protein involved in capacitation
  JOURNAL   Dev Biol 242 (2), 236-254 (2002)
   PUBMED   11820818
  REMARK    GeneRIF: sequencing, cloning, expression and characterization
REFERENCE   9  (bases 1 to 1305)
  AUTHORS   Visconti,P.E., Westbrook,V.A., Chertihin,O., Demarco,I., Sleight,S.
            and Diekman,A.B.
  TITLE     Novel signaling pathways involved in sperm acquisition of
            fertilizing capacity
  JOURNAL   J Reprod Immunol 53 (1-2), 133-150 (2002)
   PUBMED   11730911
  REMARK    Review article
REFERENCE   10 (bases 1 to 1305)
  AUTHORS   Fouquet,J.P. and Kann,M.L.
  TITLE     The cytoskeleton of mammalian spermatozoa
  JOURNAL   Biol Cell 81 (2), 89-93 (1994)
   PUBMED   7849610
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB055487.1, AK225858.1 and
            BC011996.1.
            
            On Jun 2, 2019 this sequence version replaced NM_153769.2.
            
            Summary: To reach fertilization competence, spermatozoa undergo a
            series of morphological and molecular maturational processes,
            termed capacitation, involving protein tyrosine phosphorylation and
            increased intracellular calcium. The protein encoded by this gene
            localizes to the principal piece of the sperm flagellum in
            association with the fibrous sheath and exhibits calcium-binding
            when phosphorylated during capacitation. A pseudogene on chromosome
            3 has been identified for this gene. Alternatively spliced
            transcript variants encoding distinct protein isoforms have been
            found for this gene. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (7) has an alternate splice site
            in the coding region, which results in a downstream translation
            stop codon, compared to variant 1. The encoded isoform (c) is
            shorter and has a distinct C-terminus, compared to isoform a.
            Variants 3 and 7 encode the same isoform c.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225858.1, SRR5189661.49882.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399496.8/ ENSP00000382419.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-480               DB055487.1         107-586
            481-1304            AK225858.1         481-1304
            1305-1305           BC011996.1         1376-1376
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q11.2"
     gene            1..1305
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /note="calcium binding tyrosine phosphorylation regulated"
                     /db_xref="GeneID:26256"
                     /db_xref="HGNC:HGNC:15569"
                     /db_xref="MIM:612135"
     exon            1..57
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            58..226
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1221
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /note="isoform c is encoded by transcript variant 7;
                     cancer/testis antigen 88; calcium binding
                     tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2);
                     testis-specific calcium-binding protein CBP86;
                     calcium-binding tyrosine phosphorylation-regulated
                     protein; fibrousheathin II; fibrousheathin-2;
                     calcium-binding protein 86; testis tissue sperm-binding
                     protein Li 84P"
                     /codon_start=1
                     /product="calcium-binding tyrosine
                     phosphorylation-regulated protein isoform c"
                     /protein_id="NP_722453.1"
                     /db_xref="CCDS:CCDS42420.1"
                     /db_xref="GeneID:26256"
                     /db_xref="HGNC:HGNC:15569"
                     /db_xref="MIM:612135"
                     /translation="MISSKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQEL
                     TMYRGNTTMDIKDLVKQFHQIKVEKWSEGTTPQKKLECLKEPGKTSVESKVPTQMEKS
                     TDTDEDNVTRTEYSDKTTQFPSVYAVPGTEQTEAVGGLSSKPATPKTTTPPSSPPPTA
                     VSPEFAYVPADPAQLAAQMLAMATSERGQPPPCSNMWTLYCLTDKNQQGHPSPPPAPG
                     PFPQATLYLPNPKDPQFQQHPPKVTFPTYVMGDTKKTSAPPFILVGSNVQEAQGWKPL
                     PGHAVVSQSDVLRYVAMQVPIAVPADEKYQKHTLSPQNANPPSGQDVPRPKSPVFLSV
                     AFPVEDVAKKSSGSGDKCAPFGSYGIAGEVTVTTAHKRRKAETEN"
     exon            227..280
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            281..622
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            623..1220
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1305
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1285..1290
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /note="hexamer: AATAAA"
     polyA_site      1305
                     /gene="CABYR"
                     /gene_synonym="CABYRa; CABYRc; CABYRc/d; CABYRe; CBP86;
                     CT88; FSP-2; FSP2"
                     /note="major polyA site"
ORIGIN      
        1 aggagccgcc ggcaaggggg caacgaggaa gctcttaaga gcgcggccgg aaagcagttg
       61 agttacagac atcctgccaa aatgatttct tcaaagccca gacttgtcgt accctatggc
      121 ctcaagactc tgctcgaggg aattagcaga gctgttctca aaaccaaccc atcaaacatc
      181 aaccagtttg cagcagctta ttttcaagaa cttactatgt atagagggaa tactactatg
      241 gatataaaag atctggttaa acaatttcat cagattaaag tagagaaatg gtcagaagga
      301 acgacaccac agaagaaatt agaatgttta aaagaaccag gaaaaacatc tgtagaatct
      361 aaagtaccta cccagatgga aaaatctaca gacacagacg aggacaatgt aaccagaaca
      421 gaatatagtg acaaaaccac ccagtttcca tcagtttatg ctgtgccagg cactgagcaa
      481 acggaagcag ttggtggtct ttcttccaaa ccagccaccc ctaagactac taccccaccc
      541 tcatcaccac ctccaacagc tgtctcacca gagtttgcct acgtcccagc tgacccagct
      601 cagcttgctg ctcagatgtt agcaatggca acaagtgaac gaggacaacc accaccatgt
      661 tctaacatgt ggacccttta ttgtctaact gataagaatc aacaaggtca cccatcaccg
      721 ccacctgcac ctgggccttt tccccaagca accctctatt tacctaatcc taaggatcca
      781 cagtttcagc agcatccacc aaaagtcact tttccaactt atgtgatggg cgacaccaag
      841 aagaccagtg ccccaccttt tatcttagta ggctcaaatg ttcaggaagc acagggatgg
      901 aaacctcttc ctggacatgc tgtcgtttca cagtcagatg tcttgagata tgttgcaatg
      961 caagtgccca ttgctgttcc tgcagatgag aaataccaga aacataccct aagtccccag
     1021 aatgctaatc ctccaagtgg acaagatgtc cccaggccaa aaagccctgt tttcctttct
     1081 gttgctttcc cagtagaaga tgtagctaaa aaaagttcag gatctggtga caaatgtgct
     1141 ccctttggaa gttacggtat tgctggggag gtaaccgtga ctactgctca caaacgtcgc
     1201 aaagcagaaa ctgaaaactg atccagaaat gacgctgtct gggtcaacat ttcagggagg
     1261 agtctgccac cagtgtaatg tatcaataaa cttcatgcaa gcata
//
