LOCUS       NM_001216               1546 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens carbonic anhydrase 9 (CA9), mRNA.
ACCESSION   NM_001216
VERSION     NM_001216.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Grinceviciene,S., Vaitkiene,D., Kanopiene,D., Vanseviciute
            Petkeviciene,R., Sukovas,A., Celiesiute,J., Ivanauskaite
            Didziokiene,E., Cizauskas,A., Laurinaviciene,A., Stravinskiene,D.,
            Grincevicius,J., Matulis,D. and Matuliene,J.
  TITLE     Aerobic vaginitis is associated with carbonic anhydrase IX in
            cervical intraepithelial neoplasia
  JOURNAL   Sci Rep 14 (1), 8789 (2024)
   PUBMED   38627429
  REMARK    GeneRIF: Aerobic vaginitis is associated with carbonic anhydrase IX
            in cervical intraepithelial neoplasia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Lapinova,J., Balaz,M., Balazova,L., Csaderova,L., Golias,T.,
            Zatovicova,M., Heeren,J., Pastorekova,S. and Takacova,M.
  TITLE     Unlocking the paracrine crosstalk: adipocyte-derived factors affect
            carbonic anhydrase IX expression in colon and breast cancer cells
  JOURNAL   Neoplasma 71 (2), 164-179 (2024)
   PUBMED   38766857
  REMARK    GeneRIF: Unlocking the paracrine crosstalk: adipocyte-derived
            factors affect carbonic anhydrase IX expression in colon and breast
            cancer cells.
REFERENCE   3  (bases 1 to 1546)
  AUTHORS   Shamis,S.A., Savioli,F., Ammar,A., Al-Badran,S.S.,
            Hatthakarnkul,P., Leslie,H., Mallon,E.E., Jamieson,N.B.,
            McMillan,D.C. and Edwards,J.
  TITLE     Spatial transcriptomic analysis of tumour with high and low CAIX
            expression in TNBC tissue samples using GeoMxtrade mark RNA assay
  JOURNAL   Histol Histopathol 39 (2), 177-200 (2024)
   PUBMED   37681672
  REMARK    GeneRIF: Spatial transcriptomic analysis of tumour with high and
            low CAIX expression in TNBC tissue samples using GeoMxtrade mark
            RNA assay.
REFERENCE   4  (bases 1 to 1546)
  AUTHORS   Zhao,K., Wang,X., Jin,Y., Zhu,X., Zhou,T., Yu,Y., Ji,X., Chang,Y.,
            Luo,J., Ni,X., Guo,Y. and Yu,D.
  TITLE     LncRNA ZNF674-AS1 drives cell growth and inhibits cisplatin-induced
            pyroptosis via up-regulating CA9 in neuroblastoma
  JOURNAL   Cell Death Dis 15 (1), 5 (2024)
   PUBMED   38177154
  REMARK    GeneRIF: LncRNA ZNF674-AS1 drives cell growth and inhibits
            cisplatin-induced pyroptosis via up-regulating CA9 in
            neuroblastoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1546)
  AUTHORS   Moriya,Y., Kubota,S., Iijima,Y., Takasugi,N. and Uehara,T.
  TITLE     Epigenetic Regulation of Carbonic Anhydrase 9 Expression by Nitric
            Oxide in Human Small Airway Epithelial Cells
  JOURNAL   Biol Pharm Bull 47 (6), 1119-1122 (2024)
   PUBMED   38839363
  REMARK    GeneRIF: Epigenetic Regulation of Carbonic Anhydrase 9 Expression
            by Nitric Oxide in Human Small Airway Epithelial Cells.
REFERENCE   6  (bases 1 to 1546)
  AUTHORS   Pastorekova,S., Parkkila,S., Parkkila,A.K., Opavsky,R., Zelnik,V.,
            Saarnio,J. and Pastorek,J.
  TITLE     Carbonic anhydrase IX, MN/CA IX: analysis of stomach complementary
            DNA sequence and expression in human and rat alimentary tracts
  JOURNAL   Gastroenterology 112 (2), 398-408 (1997)
   PUBMED   9024293
REFERENCE   7  (bases 1 to 1546)
  AUTHORS   Opavsky,R., Pastorekova,S., Zelnik,V., Gibadulinova,A.,
            Stanbridge,E.J., Zavada,J., Kettmann,R. and Pastorek,J.
  TITLE     Human MN/CA9 gene, a novel member of the carbonic anhydrase family:
            structure and exon to protein domain relationships
  JOURNAL   Genomics 33 (3), 480-487 (1996)
   PUBMED   8661007
REFERENCE   8  (bases 1 to 1546)
  AUTHORS   Pastorek,J., Pastorekova,S., Callebaut,I., Mornon,J.P., Zelnik,V.,
            Opavsky,R., Zat'ovicova,M., Liao,S., Portetelle,D., Stanbridge,E.J.
            et al.
  TITLE     Cloning and characterization of MN, a human tumor-associated
            protein with a domain homologous to carbonic anhydrase and a
            putative helix-loop-helix DNA binding segment
  JOURNAL   Oncogene 9 (10), 2877-2888 (1994)
   PUBMED   8084592
REFERENCE   9  (bases 1 to 1546)
  AUTHORS   Zavada,J., Zavadova,Z., Pastorekova,S., Ciampor,F., Pastorek,J. and
            Zelnik,V.
  TITLE     Expression of MaTu-MN protein in human tumor cultures and in
            clinical specimens
  JOURNAL   Int J Cancer 54 (2), 268-274 (1993)
   PUBMED   8486430
REFERENCE   10 (bases 1 to 1546)
  AUTHORS   Pastorekova,S., Zavadova,Z., Kostal,M., Babusikova,O. and Zavada,J.
  TITLE     A novel quasi-viral agent, MaTu, is a two-component system
  JOURNAL   Virology 187 (2), 620-626 (1992)
   PUBMED   1312272
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from X66839.1, AL554705.3 and
            AI023541.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001216.2.
            
            Summary: Carbonic anhydrases (CAs) are a large family of zinc
            metalloenzymes that catalyze the reversible hydration of carbon
            dioxide. They participate in a variety of biological processes,
            including respiration, calcification, acid-base balance, bone
            resorption, and the formation of aqueous humor, cerebrospinal
            fluid, saliva, and gastric acid. They show extensive diversity in
            tissue distribution and in their subcellular localization. CA IX is
            a transmembrane protein and is one of only two tumor-associated
            carbonic anhydrase isoenzymes known. It is expressed in all
            clear-cell renal cell carcinoma, but is not detected in normal
            kidney or most other normal tissues. It may be involved in cell
            proliferation and transformation. This gene was mapped to 17q21.2
            by fluorescence in situ hybridization, however, radiation hybrid
            mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014950.1, SRR3476690.458455.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378357.9/ ENSP00000367608.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               X66839.1           11-138
            129-690             AL554705.3         138-699
            691-1542            X66839.1           701-1552
            1543-1546           AI023541.1         6-9                 c
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.3"
     gene            1..1546
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /note="carbonic anhydrase 9"
                     /db_xref="GeneID:768"
                     /db_xref="HGNC:HGNC:1383"
                     /db_xref="MIM:603179"
     exon            1..435
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..1412
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /EC_number="4.2.1.1"
                     /note="carbonic dehydratase; RCC-associated protein G250;
                     pMW1; CA-IX; P54/58N; membrane antigen MN; carbonate
                     dehydratase IX; RCC-associated antigen G250; renal cell
                     carcinoma-associated antigen G250; carbonic anhydrase IX"
                     /codon_start=1
                     /product="carbonic anhydrase 9 precursor"
                     /protein_id="NP_001207.2"
                     /db_xref="CCDS:CCDS6585.1"
                     /db_xref="GeneID:768"
                     /db_xref="HGNC:HGNC:1383"
                     /db_xref="MIM:603179"
                     /translation="MAPLCPSPWLPLLIPAPAPGLTVQLLLSLLLLVPVHPQRLPRMQ
                     EDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKS
                     EEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRF
                     QSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGR
                     EYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLA
                     AFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTP
                     PCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPA
                     GVDSSPRAAEPVQLNSCLAAGDILALVFGLLFAVTSVAFLVQMRRQHRRGTKGGVSYR
                     PAEVAETGA"
     exon            436..465
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            466..636
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            637..779
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            780..872
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            873..939
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1097
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1242
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1269
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1351
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1546
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1527..1532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /note="hexamer: AATAAA"
     polyA_site      1546
                     /gene="CA9"
                     /gene_synonym="CAIX; MN"
                     /note="major polyA site"
ORIGIN      
        1 accgtgtgct gggacacccc acagtcagcc gcatggctcc cctgtgcccc agcccctggc
       61 tccctctgtt gatcccggcc cctgctccag gcctcactgt gcaactgctg ctgtcactgc
      121 tgcttctggt gcctgtccat ccccagaggt tgccccggat gcaggaggat tcccccttgg
      181 gaggaggctc ttctggggaa gatgacccac tgggcgagga ggatctgccc agtgaagagg
      241 attcacccag agaggaggat ccacccggag aggaggatct acctggagag gaggatctac
      301 ctggagagga ggatctacct gaagttaagc ctaaatcaga agaagagggc tccctgaagt
      361 tagaggatct acctactgtt gaggctcctg gagatcctca agaaccccag aataatgccc
      421 acagggacaa agaaggggat gaccagagtc attggcgcta tggaggcgac ccgccctggc
      481 cccgggtgtc cccagcctgc gcgggccgct tccagtcccc ggtggatatc cgcccccagc
      541 tcgccgcctt ctgcccggcc ctgcgccccc tggaactcct gggcttccag ctcccgccgc
      601 tcccagaact gcgcctgcgc aacaatggcc acagtgtgca actgaccctg cctcctgggc
      661 tagagatggc tctgggtccc gggcgggagt accgggctct gcagctgcat ctgcactggg
      721 gggctgcagg tcgtccgggc tcggagcaca ctgtggaagg ccaccgtttc cctgccgaga
      781 tccacgtggt tcacctcagc accgcctttg ccagagttga cgaggccttg gggcgcccgg
      841 gaggcctggc cgtgttggcc gcctttctgg aggagggccc ggaagaaaac agtgcctatg
      901 agcagttgct gtctcgcttg gaagaaatcg ctgaggaagg ctcagagact caggtcccag
      961 gactggacat atctgcactc ctgccctctg acttcagccg ctacttccaa tatgaggggt
     1021 ctctgactac accgccctgt gcccagggtg tcatctggac tgtgtttaac cagacagtga
     1081 tgctgagtgc taagcagctc cacaccctct ctgacaccct gtggggacct ggtgactctc
     1141 ggctacagct gaacttccga gcgacgcagc ctttgaatgg gcgagtgatt gaggcctcct
     1201 tccctgctgg agtggacagc agtcctcggg ctgctgagcc agtccagctg aattcctgcc
     1261 tggctgctgg tgacatccta gccctggttt ttggcctcct ttttgctgtc accagcgtcg
     1321 cgttccttgt gcagatgaga aggcagcaca gaaggggaac caaagggggt gtgagctacc
     1381 gcccagcaga ggtagccgag actggagcct agaggctgga tcttggagaa tgtgagaagc
     1441 cagccagagg catctgaggg ggagccggta actgtcctgt cctgctcatt atgccacttc
     1501 cttttaactg ccaagaaatt ttttaaaata aatatttata ataaaa
//
