LOCUS       NM_001293642            5885 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens carbonic anhydrase 12 (CA12), transcript variant 3,
            mRNA.
ACCESSION   NM_001293642
VERSION     NM_001293642.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Kim,H.J. and Hong,J.H.
  TITLE     Trafficking of carbonic anhydrase 12 and bicarbonate transporters
            by histamine stimulation mediates intracellular acidic scenario in
            lung cancer cells
  JOURNAL   J Enzyme Inhib Med Chem 38 (1), 2247181 (2023)
   PUBMED   37587861
  REMARK    GeneRIF: Trafficking of carbonic anhydrase 12 and bicarbonate
            transporters by histamine stimulation mediates intracellular acidic
            scenario in lung cancer cells.
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Ning,W.R., Jiang,D., Liu,X.C., Huang,Y.F., Peng,Z.P., Jiang,Z.Z.,
            Kang,T., Zhuang,S.M., Wu,Y. and Zheng,L.
  TITLE     Carbonic anhydrase XII mediates the survival and prometastatic
            functions of macrophages in human hepatocellular carcinoma
  JOURNAL   J Clin Invest 132 (7) (2022)
   PUBMED   35362480
  REMARK    GeneRIF: Carbonic anhydrase XII mediates the survival and
            prometastatic functions of macrophages in human hepatocellular
            carcinoma.
REFERENCE   3  (bases 1 to 5885)
  AUTHORS   Huang,T., Tang,L., Wang,H., Lin,L. and Fu,J.
  TITLE     Carbonic anhydrase 12 gene silencing reverses the sensitivity of
            paclitaxel in drug-resistant breast cancer cells
  JOURNAL   Bioengineered 12 (2), 9806-9818 (2021)
   PUBMED   34696661
  REMARK    GeneRIF: Carbonic anhydrase 12 gene silencing reverses the
            sensitivity of paclitaxel in drug-resistant breast cancer cells.
REFERENCE   4  (bases 1 to 5885)
  AUTHORS   Vergara,D., Ravaioli,S., Fonzi,E., Adamo,L., Damato,M.,
            Bravaccini,S., Pirini,F., Gaballo,A., Barbano,R., Pasculli,B.,
            Franck,J., Fournier,I., Salzet,M. and Maffia,M.
  TITLE     Carbonic Anhydrase XII Expression Is Modulated during Epithelial
            Mesenchymal Transition and Regulated through Protein Kinase C
            Signaling
  JOURNAL   Int J Mol Sci 21 (3), 715 (2020)
   PUBMED   31979064
  REMARK    GeneRIF: Carbonic Anhydrase XII Expression Is Modulated during
            Epithelial Mesenchymal Transition and Regulated through Protein
            Kinase C Signaling.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5885)
  AUTHORS   Uda,N.R., Stenner,F., Seibert,V., Herzig,P., Markuly,N., VAN
            Dijk,M., Zippelius,A. and Renner,C.
  TITLE     Humanized Monoclonal Antibody Blocking Carbonic Anhydrase 12
            Enzymatic Activity Leads to Reduced Tumor Growth In Vitro
  JOURNAL   Anticancer Res 39 (8), 4117-4128 (2019)
   PUBMED   31366496
  REMARK    GeneRIF: Our newly identified humanized CA12-antibody with
            anti-cancer activity, represents a new tool for the treatment of
            CA12-positive tumors.
REFERENCE   6  (bases 1 to 5885)
  AUTHORS   Karhumaa,P., Parkkila,S., Tureci,O., Waheed,A., Grubb,J.H.,
            Shah,G., Parkkila,A., Kaunisto,K., Tapanainen,J., Sly,W.S. and
            Rajaniemi,H.
  TITLE     Identification of carbonic anhydrase XII as the membrane isozyme
            expressed in the normal human endometrial epithelium
  JOURNAL   Mol Hum Reprod 6 (1), 68-74 (2000)
   PUBMED   10611263
REFERENCE   7  (bases 1 to 5885)
  AUTHORS   Fujikawa-Adachi,K., Nishimori,I., Taguchi,T. and Onishi,S.
  TITLE     Human carbonic anhydrase XIV (CA14): cDNA cloning, mRNA expression,
            and mapping to chromosome 1
  JOURNAL   Genomics 61 (1), 74-81 (1999)
   PUBMED   10512682
REFERENCE   8  (bases 1 to 5885)
  AUTHORS   Ivanov,S.V., Kuzmin,I., Wei,M.H., Pack,S., Geil,L., Johnson,B.E.,
            Stanbridge,E.J. and Lerman,M.I.
  TITLE     Down-regulation of transmembrane carbonic anhydrases in renal cell
            carcinoma cell lines by wild-type von Hippel-Lindau transgenes
  JOURNAL   Proc Natl Acad Sci U S A 95 (21), 12596-12601 (1998)
   PUBMED   9770531
REFERENCE   9  (bases 1 to 5885)
  AUTHORS   Tureci,O., Sahin,U., Vollmar,E., Siemer,S., Gottert,E., Seitz,G.,
            Parkkila,A.K., Shah,G.N., Grubb,J.H., Pfreundschuh,M. and Sly,W.S.
  TITLE     Human carbonic anhydrase XII: cDNA cloning, expression, and
            chromosomal localization of a carbonic anhydrase gene that is
            overexpressed in some renal cell cancers
  JOURNAL   Proc Natl Acad Sci U S A 95 (13), 7608-7613 (1998)
   PUBMED   9636197
REFERENCE   10 (bases 1 to 5885)
  AUTHORS   Sahin,U., Tureci,O., Schmitt,H., Cochlovius,B., Johannes,T.,
            Schmits,R., Stenner,F., Luo,G., Schobert,I. and Pfreundschuh,M.
  TITLE     Human neoplasms elicit multiple specific immune responses in the
            autologous host
  JOURNAL   Proc Natl Acad Sci U S A 92 (25), 11810-11813 (1995)
   PUBMED   8524854
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK096845.1, AC087525.6 and
            AC016207.9.
            
            On Aug 14, 2020 this sequence version replaced NM_001293642.1.
            
            Summary: Carbonic anhydrases (CAs) are a large family of zinc
            metalloenzymes that catalyze the reversible hydration of carbon
            dioxide. They participate in a variety of biological processes,
            including respiration, calcification, acid-base balance, bone
            resorption, and the formation of aqueous humor, cerebrospinal
            fluid, saliva, and gastric acid. This gene product is a type I
            membrane protein that is highly expressed in normal tissues, such
            as kidney, colon and pancreas, and has been found to be
            overexpressed in 10% of clear cell renal carcinomas. Three
            transcript variants encoding different isoforms have been
            identified for this gene. [provided by RefSeq, Jun 2014].
            
            Transcript Variant: This variant (3) lacks two alternate in-frame
            exons compared to variant 1. The resulting isoform (3) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK096845.1, SRR14038197.1491734.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2556              AK096845.1         1-2556
            2557-4632           AC087525.6         42064-44139         c
            4633-5885           AC016207.9         1-1253
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.2"
     gene            1..5885
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="carbonic anhydrase 12"
                     /db_xref="GeneID:771"
                     /db_xref="HGNC:HGNC:1371"
                     /db_xref="MIM:603263"
     exon            1..211
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..978
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /EC_number="4.2.1.1"
                     /note="isoform 3 precursor is encoded by transcript
                     variant 3; carbonic dehydratase; carbonate dehydratase
                     XII; carbonic anhydrase XII; tumor antigen HOM-RCC-3.1.3"
                     /codon_start=1
                     /product="carbonic anhydrase 12 isoform 3 precursor"
                     /protein_id="NP_001280571.1"
                     /db_xref="CCDS:CCDS76767.1"
                     /db_xref="GeneID:771"
                     /db_xref="HGNC:HGNC:1371"
                     /db_xref="MIM:603263"
                     /translation="MPRRSLHAAAVLLLVILKEQPSSPAPVNGSKWTYFVKLNLPSDM
                     HIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASN
                     KSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYR
                     YRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQ
                     KFDERLVYTSFSQGIILSLALAGILGICIVVVVSIWLFRRKSIKKGDNKGVIYKPATK
                     METEAHA"
     exon            212..232
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            233..375
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            376..471
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            472..535
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            536..693
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            694..820
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            821..905
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            906..5885
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3713..3718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="hexamer: TATAAA"
     polyA_site      3732
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="major polyA site"
     regulatory      5170..5175
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="hexamer: AATAAA"
     polyA_site      5193
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
     regulatory      5864..5869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="hexamer: CATAAA"
     polyA_site      5885
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
ORIGIN      
        1 gaagggcgga cgtactcgcc acggcaccca ggctgcgcgc acgcggtccc ggtgtgcagc
       61 tggagagcga gcggccaccg ggagcccccg gcacagcccg cgcccgcccc gcaggagccc
      121 gcgaagatgc cccggcgcag cctgcacgcg gcggccgtgc tcctgctggt gatcttaaag
      181 gaacagcctt ccagcccggc cccagtgaac ggttccaagt ggacttattt tgtgaagctg
      241 aacctgccct cggacatgca catccagggc ctccagtctc gctacagtgc cacgcagctg
      301 cacctgcact gggggaaccc gaatgacccg cacggctctg agcacaccgt cagcggacag
      361 cacttcgccg ccgagctgca cattgtccat tataactcag acctttatcc tgacgccagc
      421 actgccagca acaagtcaga aggcctcgct gtcctggctg ttctcattga gatgggctcc
      481 ttcaatccgt cctatgacaa gatcttcagt caccttcaac atgtaaagta caaaggccag
      541 gaagcattcg tcccgggatt caacattgaa gagctgcttc cggagaggac cgctgaatat
      601 taccgctacc gggggtccct gaccacaccc ccttgcaacc ccactgtgct ctggacagtt
      661 ttccgaaacc ccgtgcaaat ttcccaggag cagctgctgg ctttggagac agccctgtac
      721 tgcacacaca tggacgaccc ttcccccaga gaaatgatca acaacttccg gcaggtccag
      781 aagttcgatg agaggctggt atacacctcc ttctcccaag gcatcatcct ctcactggcc
      841 ctggctggca ttcttggcat ctgtattgtg gtggtggtgt ccatttggct tttcagaagg
      901 aagagtatca aaaaaggtga taacaaggga gtcatttaca agccagccac caagatggag
      961 actgaggccc acgcttgagg tccccggagc tcccgggcac atccaggaag gaccttgctt
     1021 tggaccctac acacttcggc tctctggaca cttgcgacac ctcaaggtgt tctctgtagc
     1081 tcaatctgca aacatgccag gcctcaggga tcctctgctg ggtgcctcct tgccttggga
     1141 ccatggccac cccagagcca tccgatcgat ggatgggatg cactctcaga ccaagcagca
     1201 ggaattcaaa gctgcttgct gtaactgtgt gagattgtga agtggtctga attctggaat
     1261 cacaaaccaa gccatgctgg tgggccatta atggttggaa aacactttca tccggggctt
     1321 tgccagagcg tgctttcaag tgtcctggaa attctgctgc ttctccaagc tttcagacaa
     1381 gaatgtgcac tctctgctta ggttttgctt gggaaactca acttctttcc tctggagacg
     1441 gggcatctcc ctctgatttc cttctgctat gacaaaacct ttaatctgca ccttacaact
     1501 cggggacaaa tggggacagg aaggatcaag ttgtagagag aaaaagaaaa caagagatat
     1561 acattgtgat atattaggga cactttcaca gtcctgtcct ctggatcaca gacactgcac
     1621 agaccttagg gaatggcagg ttcaagttcc acttcttggt ggggatgaga agggagagag
     1681 agctagaggg acaaagagaa tgagaagaca tggatgatct gggagagtct cactttggaa
     1741 tcagaattgg aatcacattc tgtttatcaa gccataatgt aaggacagaa taatacaata
     1801 ttaagtccaa atccaacctc ctgtcagtgg agcagttatg ttttatactc tacagatttt
     1861 acaaataatg aggctgttcc ttgaaaatgt gttgttgctg tgtcctggag gagacatgag
     1921 ttccgagatg acccaatctg cctttgaatc tggaggaaat aggcagaaac aaaatgactg
     1981 tagaacttat tctctgtagg ccaaatttca tttcagccac ttctgcagga tccctactgc
     2041 caacctggaa tggagacttt tatctacttc tctctctctg aagatgtcaa atcgtggttt
     2101 agatcaaata tatttcaagc tataaaagca ggaggttatc tgtgcagggg gctggcatca
     2161 tgtatttagg ggcaagtaat aatggaatgc tactaagata ctccatattc ttccccgaat
     2221 cacacagaca gtttctgaca ggcgcaactc ctccattttc ctcccgcagg tgagaaccct
     2281 gtggagatga gtcagtgcca tgactgagaa ggaaccgacc cctagttgag agcaccttgc
     2341 agttccccga gaactttctg attcacagtc tcattttgac agcatgaaat gtcctcttga
     2401 agcatagctt tttaaatatc tttttccttc tactcctccc tctgactcta agaattctct
     2461 cttctggaat cgcttgaacc caggaggcgg aggttgcagt aagccaaggt catgccactg
     2521 cactctagcc tgggtgacag agcgagactc catctcaaaa aaaaaaaaaa aaaaattatt
     2581 ctgtaccatc acaacttttc acaacgatgg caagccttat gtcttgggag cctgttttgc
     2641 taggcaaagt tacaagtgac ctaatgggag ctcaaatgtg tgtgtgtctc tctgtgtgtt
     2701 tgtgtgtgtg tgtgcactca agacctctaa cagcctcgaa gcctggggtg gcatcccggc
     2761 cttgccatta gcatgcctca tgcatcatca gatgacaagg acaaccctca tgacgaagca
     2821 acatgaatta gggggcctct tggccttggt ccaaaattgt caatcagaaa tgaacataaa
     2881 ggactccaga gcagtgggac tgtctgtcaa aagactctgt atatcttttg tggatgagtt
     2941 ttgtgagaga acagagagac cattgtacct ggcacaaggg ctgttcatga aaagggagac
     3001 ttactgggag gtgcaagaca gtggcatttc tcctctcctc ttgctgctca gcacagccct
     3061 ggattgcagc cccgaggctg agaccagaca aagcccggga ggcagaaaga tgctccaaga
     3121 accaacacta tcaatgtctt tgcaaatcct cacaggattc ctgtgggtcc agctttggaa
     3181 ctgggaaacc tttcttcgga tccgcactca ttccactgat gccagctgcc cctgaaggat
     3241 gccagtactg tggtgtgtga gtctcagcag ccgcccacac gctcctaact ctgctgcatg
     3301 gcagatgcct aggtggaaat agcaaaaaca aggcccaggc tggggccagg gccagagggg
     3361 aaggccctgg attctcactc atgtgagatc ttgaatctct ttctttgttc tgtttgttta
     3421 gttagtatca tctggtaaaa tagttaaaaa acaacaaaaa actctgtatc tgtttctagc
     3481 atgtgctgca ttgactctat taatcacatt tcaaattcac cctacattcc tctcctcttc
     3541 actagcctct ctgaaggtgt cctggccagc cctggagaag cactggtgtc tgcagcaccc
     3601 ctcagttcct gtgcctcagc ccacaggcca ctgtgataat ggtctgttta gcacttctgt
     3661 atttattgta agaatgatta taatgaagat acacactgta actacaagaa attataaatg
     3721 tttttcacat caggctgttc tttttttttt tttggaggcg aggttaaagc attactattt
     3781 gcaaagcact ctgtagctcc ctgttatggg gataggtaac taatcagaat aataatgtca
     3841 ctcgcatcca cttcttagaa cctggctcca aaggaaaata agctgataga ctcaatcact
     3901 ttcctgagga tggaggccta tggcatgtgt ggctgcaggt cgcgaagctg cttaatggtg
     3961 ctgggaagcc tagaggaatt aaataaagac cctggaggag gtgggatcgg agctgcacct
     4021 tgaacaaata gctcagattt gaacagatgg atggaaaaga gggaggtgtt cccaaccaag
     4081 gggacatgat acagggaaca ggtcctatgt ctctttactc ctgagagtat taaacctagt
     4141 aggtgggcag cccctcttgg ttgcctgtta ccttattttg aattcttttt gcaaaacata
     4201 ctatcacccg tccaaataat cttttgtcta aatccagact tcacattctg actggggcaa
     4261 aaagaggcag tccagtaaag gttataaaat aggtaccatc attgtttgcc ttatttagaa
     4321 aagcatccaa ttttcactat aatcacccta agcctgagag aggtgaactg ttcaagggta
     4381 ctttggctcc agtgggtgac agtacctggc ccagctttgg aattgaaaca tttctgatgg
     4441 tctgtactct gctagaacac aggatgcttc tgctctccct gctctggcat cctgccaggt
     4501 gtcatggcca cgcacaggca tgaagacagc cgggaagcca gagttcccac gaagcactca
     4561 ctccttggac ttgctcccac cccactgggg agagcactcc tggagcagga aatgagcatc
     4621 tctcatctcc ctgaattcca catccactgg ctgaatgatc agggaggcat agcagtgaga
     4681 gccataggtc ggcagaggga actcaggccc tcctttagga tggccatcac ctcatctcaa
     4741 tccagccaaa tcaaccatct agagcacaca ggccgagaga aatgtaataa aatataacat
     4801 gagacacgta tgaaatttaa actttccggt agccacacta gaaaaaggta aaaaaaaaaa
     4861 aaccagtgac attaatttaa atttaatttt agataatcca acagatctaa aatactatca
     4921 tttccacatg taaccagtat tttcatgtgt ttttttctgc agtaaatctt tgaaatctgg
     4981 tgtgtgtttt acactgacag cacactcgat tcagatgcta gattttcctt gtagatattt
     5041 ggtcgttatt tagatttcat aaaatttaca gtttaaaaga ttccaatatc cgagttgctc
     5101 caaacacact tagaagttgt tttccaaata atggaatcaa tgatcggctt ttagatgtaa
     5161 acttattaaa ataaagtagc gtttctcagt taagtcacat tccaagcgct caagatccat
     5221 gccctgcgca gacccggacg acgaacccga ctttcagaga ctgagggttc tgcctagaga
     5281 gctcccacag aaatcagccc gagaagaaag ccttgtgggt gaagccaggg agggagacag
     5341 gagggcagga ggaaaatcaa gcctaaatga gcacatcgtg gacgccgctc ttcctctccc
     5401 cacatctcct cgcctcgccg cagtgggccc ggctgtgccc caggtccccg cggtcgggag
     5461 tgaatggcgc tcgggtggca ggcacgtggg cacctggtga actgcaggac cctggcaccg
     5521 ccaccccctc gcccctacac acacaggagt gcccctcccg ttgtcacgtg gccttcctgc
     5581 tgggtatctg gagcagttgc agcctcgctt ccggaaggaa acggggcctc tggggcacag
     5641 ggaccacctg ccagtgcgtt ccagccgggc tgccatccac actggagacc gccccgcacg
     5701 tggtcctcgg gctcggattc agctgtcgca ggatgtgagg ggaaaggtat gtggcccgac
     5761 aatgccaccg agggtgtccc cgggctccac cccgtgcctc ttcattatag aatgactggg
     5821 cagagataag ggccagatta ctttgctgta ttgaataatt caccataaaa atgattgcaa
     5881 ccagg
//
