LOCUS       NM_001218               6098 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens carbonic anhydrase 12 (CA12), transcript variant 1,
            mRNA.
ACCESSION   NM_001218 NM_017689
VERSION     NM_001218.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Kim,H.J. and Hong,J.H.
  TITLE     Trafficking of carbonic anhydrase 12 and bicarbonate transporters
            by histamine stimulation mediates intracellular acidic scenario in
            lung cancer cells
  JOURNAL   J Enzyme Inhib Med Chem 38 (1), 2247181 (2023)
   PUBMED   37587861
  REMARK    GeneRIF: Trafficking of carbonic anhydrase 12 and bicarbonate
            transporters by histamine stimulation mediates intracellular acidic
            scenario in lung cancer cells.
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Ning,W.R., Jiang,D., Liu,X.C., Huang,Y.F., Peng,Z.P., Jiang,Z.Z.,
            Kang,T., Zhuang,S.M., Wu,Y. and Zheng,L.
  TITLE     Carbonic anhydrase XII mediates the survival and prometastatic
            functions of macrophages in human hepatocellular carcinoma
  JOURNAL   J Clin Invest 132 (7) (2022)
   PUBMED   35362480
  REMARK    GeneRIF: Carbonic anhydrase XII mediates the survival and
            prometastatic functions of macrophages in human hepatocellular
            carcinoma.
REFERENCE   3  (bases 1 to 6098)
  AUTHORS   Huang,T., Tang,L., Wang,H., Lin,L. and Fu,J.
  TITLE     Carbonic anhydrase 12 gene silencing reverses the sensitivity of
            paclitaxel in drug-resistant breast cancer cells
  JOURNAL   Bioengineered 12 (2), 9806-9818 (2021)
   PUBMED   34696661
  REMARK    GeneRIF: Carbonic anhydrase 12 gene silencing reverses the
            sensitivity of paclitaxel in drug-resistant breast cancer cells.
REFERENCE   4  (bases 1 to 6098)
  AUTHORS   Vergara,D., Ravaioli,S., Fonzi,E., Adamo,L., Damato,M.,
            Bravaccini,S., Pirini,F., Gaballo,A., Barbano,R., Pasculli,B.,
            Franck,J., Fournier,I., Salzet,M. and Maffia,M.
  TITLE     Carbonic Anhydrase XII Expression Is Modulated during Epithelial
            Mesenchymal Transition and Regulated through Protein Kinase C
            Signaling
  JOURNAL   Int J Mol Sci 21 (3), 715 (2020)
   PUBMED   31979064
  REMARK    GeneRIF: Carbonic Anhydrase XII Expression Is Modulated during
            Epithelial Mesenchymal Transition and Regulated through Protein
            Kinase C Signaling.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6098)
  AUTHORS   Uda,N.R., Stenner,F., Seibert,V., Herzig,P., Markuly,N., VAN
            Dijk,M., Zippelius,A. and Renner,C.
  TITLE     Humanized Monoclonal Antibody Blocking Carbonic Anhydrase 12
            Enzymatic Activity Leads to Reduced Tumor Growth In Vitro
  JOURNAL   Anticancer Res 39 (8), 4117-4128 (2019)
   PUBMED   31366496
  REMARK    GeneRIF: Our newly identified humanized CA12-antibody with
            anti-cancer activity, represents a new tool for the treatment of
            CA12-positive tumors.
REFERENCE   6  (bases 1 to 6098)
  AUTHORS   Karhumaa,P., Parkkila,S., Tureci,O., Waheed,A., Grubb,J.H.,
            Shah,G., Parkkila,A., Kaunisto,K., Tapanainen,J., Sly,W.S. and
            Rajaniemi,H.
  TITLE     Identification of carbonic anhydrase XII as the membrane isozyme
            expressed in the normal human endometrial epithelium
  JOURNAL   Mol Hum Reprod 6 (1), 68-74 (2000)
   PUBMED   10611263
REFERENCE   7  (bases 1 to 6098)
  AUTHORS   Fujikawa-Adachi,K., Nishimori,I., Taguchi,T. and Onishi,S.
  TITLE     Human carbonic anhydrase XIV (CA14): cDNA cloning, mRNA expression,
            and mapping to chromosome 1
  JOURNAL   Genomics 61 (1), 74-81 (1999)
   PUBMED   10512682
REFERENCE   8  (bases 1 to 6098)
  AUTHORS   Ivanov,S.V., Kuzmin,I., Wei,M.H., Pack,S., Geil,L., Johnson,B.E.,
            Stanbridge,E.J. and Lerman,M.I.
  TITLE     Down-regulation of transmembrane carbonic anhydrases in renal cell
            carcinoma cell lines by wild-type von Hippel-Lindau transgenes
  JOURNAL   Proc Natl Acad Sci U S A 95 (21), 12596-12601 (1998)
   PUBMED   9770531
REFERENCE   9  (bases 1 to 6098)
  AUTHORS   Tureci,O., Sahin,U., Vollmar,E., Siemer,S., Gottert,E., Seitz,G.,
            Parkkila,A.K., Shah,G.N., Grubb,J.H., Pfreundschuh,M. and Sly,W.S.
  TITLE     Human carbonic anhydrase XII: cDNA cloning, expression, and
            chromosomal localization of a carbonic anhydrase gene that is
            overexpressed in some renal cell cancers
  JOURNAL   Proc Natl Acad Sci U S A 95 (13), 7608-7613 (1998)
   PUBMED   9636197
REFERENCE   10 (bases 1 to 6098)
  AUTHORS   Sahin,U., Tureci,O., Schmitt,H., Cochlovius,B., Johannes,T.,
            Schmits,R., Stenner,F., Luo,G., Schobert,I. and Pfreundschuh,M.
  TITLE     Human neoplasms elicit multiple specific immune responses in the
            autologous host
  JOURNAL   Proc Natl Acad Sci U S A 92 (25), 11810-11813 (1995)
   PUBMED   8524854
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC023981.1, AC087525.6 and
            AC016207.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001218.4.
            
            Summary: Carbonic anhydrases (CAs) are a large family of zinc
            metalloenzymes that catalyze the reversible hydration of carbon
            dioxide. They participate in a variety of biological processes,
            including respiration, calcification, acid-base balance, bone
            resorption, and the formation of aqueous humor, cerebrospinal
            fluid, saliva, and gastric acid. This gene product is a type I
            membrane protein that is highly expressed in normal tissues, such
            as kidney, colon and pancreas, and has been found to be
            overexpressed in 10% of clear cell renal carcinomas. Three
            transcript variants encoding different isoforms have been
            identified for this gene. [provided by RefSeq, Jun 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC023981.1, SRR14038195.2733947.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000178638.8/ ENSP00000178638.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2767              BC023981.1         5-2771
            2768-4845           AC087525.6         42064-44141         c
            4846-6098           AC016207.9         1-1253
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.2"
     gene            1..6098
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="carbonic anhydrase 12"
                     /db_xref="GeneID:771"
                     /db_xref="HGNC:HGNC:1371"
                     /db_xref="MIM:603263"
     exon            1..211
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..1191
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /EC_number="4.2.1.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; carbonic dehydratase; carbonate dehydratase
                     XII; carbonic anhydrase XII; tumor antigen HOM-RCC-3.1.3"
                     /codon_start=1
                     /product="carbonic anhydrase 12 isoform 1 precursor"
                     /protein_id="NP_001209.1"
                     /db_xref="CCDS:CCDS10185.1"
                     /db_xref="GeneID:771"
                     /db_xref="HGNC:HGNC:1371"
                     /db_xref="MIM:603263"
                     /translation="MPRRSLHAAAVLLLVILKEQPSSPAPVNGSKWTYFGPDGENSWS
                     KKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPS
                     DMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTA
                     SNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEY
                     YRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQ
                     VQKFDERLVYTSFSQVQVCTAAGLSLGIILSLALAGILGICIVVVVSIWLFRRKSIKK
                     GDNKGVIYKPATKMETEAHA"
     exon            212..232
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            233..412
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            413..555
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            556..651
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            652..715
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            716..873
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1000
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1033
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1118
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..6098
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3926..3931
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="hexamer: TATAAA"
     polyA_site      3945
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="major polyA site"
     regulatory      5383..5388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="hexamer: AATAAA"
     polyA_site      5406
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
     regulatory      6077..6082
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
                     /note="hexamer: CATAAA"
     polyA_site      6098
                     /gene="CA12"
                     /gene_synonym="CA-XII; CAXII; HsT18816; T18816"
ORIGIN      
        1 gaagggcgga cgtactcgcc acggcaccca ggctgcgcgc acgcggtccc ggtgtgcagc
       61 tggagagcga gcggccaccg ggagcccccg gcacagcccg cgcccgcccc gcaggagccc
      121 gcgaagatgc cccggcgcag cctgcacgcg gcggccgtgc tcctgctggt gatcttaaag
      181 gaacagcctt ccagcccggc cccagtgaac ggttccaagt ggacttattt tggtcctgat
      241 ggggagaata gctggtccaa gaagtacccg tcgtgtgggg gcctgctgca gtcccccata
      301 gacctgcaca gtgacatcct ccagtatgac gccagcctca cgcccctcga gttccaaggc
      361 tacaatctgt ctgccaacaa gcagtttctc ctgaccaaca atggccattc agtgaagctg
      421 aacctgccct cggacatgca catccagggc ctccagtctc gctacagtgc cacgcagctg
      481 cacctgcact gggggaaccc gaatgacccg cacggctctg agcacaccgt cagcggacag
      541 cacttcgccg ccgagctgca cattgtccat tataactcag acctttatcc tgacgccagc
      601 actgccagca acaagtcaga aggcctcgct gtcctggctg ttctcattga gatgggctcc
      661 ttcaatccgt cctatgacaa gatcttcagt caccttcaac atgtaaagta caaaggccag
      721 gaagcattcg tcccgggatt caacattgaa gagctgcttc cggagaggac cgctgaatat
      781 taccgctacc gggggtccct gaccacaccc ccttgcaacc ccactgtgct ctggacagtt
      841 ttccgaaacc ccgtgcaaat ttcccaggag cagctgctgg ctttggagac agccctgtac
      901 tgcacacaca tggacgaccc ttcccccaga gaaatgatca acaacttccg gcaggtccag
      961 aagttcgatg agaggctggt atacacctcc ttctcccaag tgcaagtctg tactgcggca
     1021 ggactgagtc tgggcatcat cctctcactg gccctggctg gcattcttgg catctgtatt
     1081 gtggtggtgg tgtccatttg gcttttcaga aggaagagta tcaaaaaagg tgataacaag
     1141 ggagtcattt acaagccagc caccaagatg gagactgagg cccacgcttg aggtccccgg
     1201 agctcccggg cacatccagg aaggaccttg ctttggaccc tacacacttc ggctctctgg
     1261 acacttgcga cacctcaagg tgttctctgt agctcaatct gcaaacatgc caggcctcag
     1321 ggatcctctg ctgggtgcct ccttgccttg ggaccatggc caccccagag ccatccgatc
     1381 gatggatggg atgcactctc agaccaagca gcaggaattc aaagctgctt gctgtaactg
     1441 tgtgagattg tgaagtggtc tgaattctgg aatcacaaac caagccatgc tggtgggcca
     1501 ttaatggttg gaaaacactt tcatccgggg ctttgccaga gcgtgctttc aagtgtcctg
     1561 gaaattctgc tgcttctcca agctttcaga caagaatgtg cactctctgc ttaggttttg
     1621 cttgggaaac tcaacttctt tcctctggag acggggcatc tccctctgat ttccttctgc
     1681 tatgacaaaa cctttaatct gcaccttaca actcggggac aaatggggac aggaaggatc
     1741 aagttgtaga gagaaaaaga aaacaagaga tatacattgt gatatattag ggacactttc
     1801 acagtcctgt cctctggatc acagacactg cacagacctt agggaatggc aggttcaagt
     1861 tccacttctt ggtggggatg agaagggaga gagagctaga gggacaaaga gaatgagaag
     1921 acatggatga tctgggagag tctcactttg gaatcagaat tggaatcaca ttctgtttat
     1981 caagccataa tgtaaggaca gaataataca atattaagtc caaatccaac ctcctgtcag
     2041 tggagcagtt atgttttata ctctacagat tttacaaata atgaggctgt tccttgaaaa
     2101 tgtgttgttg ctgtgtcctg gaggagacat gagttccgag atgacccaat ctgcctttga
     2161 atctggagga aataggcaga aacaaaatga ctgtagaact tattctctgt aggccaaatt
     2221 tcatttcagc cacttctgca ggatccctac tgccaacctg gaatggagac ttttatctac
     2281 ttctctctct ctgaagatgt caaatcgtgg tttagatcaa atatatttca agctataaaa
     2341 gcaggaggtt atctgtgcag ggggctggca tcatgtattt aggggcaagt aataatggaa
     2401 tgctactaag atactccata ttcttccccg aatcacacag acagtttctg acaggcgcaa
     2461 ctcctccatt ttcctcccgc aggtgagaac cctgtggaga tgagtcagtg ccatgactga
     2521 gaaggaaccg acccctagtt gagagcacct tgcagttccc cgagaacttt ctgattcaca
     2581 gtctcatttt gacagcatga aatgtcctct tgaagcatag ctttttaaat atctttttcc
     2641 ttctactcct ccctctgact ctaagaattc tctcttctgg aatcgcttga acccaggagg
     2701 cggaggttgc agtaagccaa ggtcatgcca ctgcactcta gcctgggtga cagagcgaga
     2761 ctccatctca aaaaaaaaaa aaaaaaaatt attctgtacc atcacaactt ttcacaacga
     2821 tggcaagcct tatgtcttgg gagcctgttt tgctaggcaa agttacaagt gacctaatgg
     2881 gagctcaaat gtgtgtgtgt ctctctgtgt gtttgtgtgt gtgtgtgcac tcaagacctc
     2941 taacagcctc gaagcctggg gtggcatccc ggccttgcca ttagcatgcc tcatgcatca
     3001 tcagatgaca aggacaaccc tcatgacgaa gcaacatgaa ttagggggcc tcttggcctt
     3061 ggtccaaaat tgtcaatcag aaatgaacat aaaggactcc agagcagtgg gactgtctgt
     3121 caaaagactc tgtatatctt ttgtggatga gttttgtgag agaacagaga gaccattgta
     3181 cctggcacaa gggctgttca tgaaaaggga gacttactgg gaggtgcaag acagtggcat
     3241 ttctcctctc ctcttgctgc tcagcacagc cctggattgc agccccgagg ctgagaccag
     3301 acaaagcccg ggaggcagaa agatgctcca agaaccaaca ctatcaatgt ctttgcaaat
     3361 cctcacagga ttcctgtggg tccagctttg gaactgggaa acctttcttc ggatccgcac
     3421 tcattccact gatgccagct gcccctgaag gatgccagta ctgtggtgtg tgagtctcag
     3481 cagccgccca cacgctccta actctgctgc atggcagatg cctaggtgga aatagcaaaa
     3541 acaaggccca ggctggggcc agggccagag gggaaggccc tggattctca ctcatgtgag
     3601 atcttgaatc tctttctttg ttctgtttgt ttagttagta tcatctggta aaatagttaa
     3661 aaaacaacaa aaaactctgt atctgtttct agcatgtgct gcattgactc tattaatcac
     3721 atttcaaatt caccctacat tcctctcctc ttcactagcc tctctgaagg tgtcctggcc
     3781 agccctggag aagcactggt gtctgcagca cccctcagtt cctgtgcctc agcccacagg
     3841 ccactgtgat aatggtctgt ttagcacttc tgtatttatt gtaagaatga ttataatgaa
     3901 gatacacact gtaactacaa gaaattataa atgtttttca catcaggctg ttcttttttt
     3961 ttttttggag gcgaggttaa agcattacta tttgcaaagc actctgtagc tccctgttat
     4021 ggggataggt aactaatcag aataataatg tcactcgcat ccacttctta gaacctggct
     4081 ccaaaggaaa ataagctgat agactcaatc actttcctga ggatggaggc ctatggcatg
     4141 tgtggctgca ggtcgcgaag ctgcttaatg gtgctgggaa gcctagagga attaaataaa
     4201 gaccctggag gaggtgggat cggagctgca ccttgaacaa atagctcaga tttgaacaga
     4261 tggatggaaa agagggaggt gttcccaacc aaggggacat gatacaggga acaggtccta
     4321 tgtctcttta ctcctgagag tattaaacct agtaggtggg cagcccctct tggttgcctg
     4381 ttaccttatt ttgaattctt tttgcaaaac atactatcac ccgtccaaat aatcttttgt
     4441 ctaaatccag acttcacatt ctgactgggg caaaaagagg cagtccagta aaggttataa
     4501 aataggtacc atcattgttt gccttattta gaaaagcatc caattttcac tataatcacc
     4561 ctaagcctga gagaggtgaa ctgttcaagg gtactttggc tccagtgggt gacagtacct
     4621 ggcccagctt tggaattgaa acatttctga tggtctgtac tctgctagaa cacaggatgc
     4681 ttctgctctc cctgctctgg catcctgcca ggtgtcatgg ccacgcacag gcatgaagac
     4741 agccgggaag ccagagttcc cacgaagcac tcactccttg gacttgctcc caccccactg
     4801 gggagagcac tcctggagca ggaaatgagc atctctcatc tccctgaatt ccacatccac
     4861 tggctgaatg atcagggagg catagcagtg agagccatag gtcggcagag ggaactcagg
     4921 ccctccttta ggatggccat cacctcatct caatccagcc aaatcaacca tctagagcac
     4981 acaggccgag agaaatgtaa taaaatataa catgagacac gtatgaaatt taaactttcc
     5041 ggtagccaca ctagaaaaag gtaaaaaaaa aaaaaccagt gacattaatt taaatttaat
     5101 tttagataat ccaacagatc taaaatacta tcatttccac atgtaaccag tattttcatg
     5161 tgtttttttc tgcagtaaat ctttgaaatc tggtgtgtgt tttacactga cagcacactc
     5221 gattcagatg ctagattttc cttgtagata tttggtcgtt atttagattt cataaaattt
     5281 acagtttaaa agattccaat atccgagttg ctccaaacac acttagaagt tgttttccaa
     5341 ataatggaat caatgatcgg cttttagatg taaacttatt aaaataaagt agcgtttctc
     5401 agttaagtca cattccaagc gctcaagatc catgccctgc gcagacccgg acgacgaacc
     5461 cgactttcag agactgaggg ttctgcctag agagctccca cagaaatcag cccgagaaga
     5521 aagccttgtg ggtgaagcca gggagggaga caggagggca ggaggaaaat caagcctaaa
     5581 tgagcacatc gtggacgccg ctcttcctct ccccacatct cctcgcctcg ccgcagtggg
     5641 cccggctgtg ccccaggtcc ccgcggtcgg gagtgaatgg cgctcgggtg gcaggcacgt
     5701 gggcacctgg tgaactgcag gaccctggca ccgccacccc ctcgccccta cacacacagg
     5761 agtgcccctc ccgttgtcac gtggccttcc tgctgggtat ctggagcagt tgcagcctcg
     5821 cttccggaag gaaacggggc ctctggggca cagggaccac ctgccagtgc gttccagccg
     5881 ggctgccatc cacactggag accgccccgc acgtggtcct cgggctcgga ttcagctgtc
     5941 gcaggatgtg aggggaaagg tatgtggccc gacaatgcca ccgagggtgt ccccgggctc
     6001 caccccgtgc ctcttcatta tagaatgact gggcagagat aagggccaga ttactttgct
     6061 gtattgaata attcaccata aaaatgattg caaccagg
//
