LOCUS       NM_001040032            1042 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens chromosome 8 open reading frame 74 (C8orf74), mRNA.
ACCESSION   NM_001040032 XM_114621 XM_938061
VERSION     NM_001040032.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Weimann,M., Grossmann,A., Woodsmith,J., Ozkan,Z., Birth,P.,
            Meierhofer,D., Benlasfer,N., Valovka,T., Timmermann,B.,
            Wanker,E.E., Sauer,S. and Stelzl,U.
  TITLE     A Y2H-seq approach defines the human protein methyltransferase
            interactome
  JOURNAL   Nat Methods 10 (4), 339-342 (2013)
   PUBMED   23455924
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC105001.3.
            
            On Nov 23, 2018 this sequence version replaced NM_001040032.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065837.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000304519.10/ ENSP00000307129.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                AC105001.3         28521-28606
            87-279              AC105001.3         30539-30731
            280-686             AC105001.3         53492-53898
            687-1042            AC105001.3         56128-56483
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p23.1"
     gene            1..1042
                     /gene="C8orf74"
                     /note="chromosome 8 open reading frame 74"
                     /db_xref="GeneID:203076"
                     /db_xref="HGNC:HGNC:32296"
     exon            1..86
                     /gene="C8orf74"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..923
                     /gene="C8orf74"
                     /codon_start=1
                     /product="uncharacterized protein C8orf74"
                     /protein_id="NP_001035121.2"
                     /db_xref="CCDS:CCDS47800.1"
                     /db_xref="GeneID:203076"
                     /db_xref="HGNC:HGNC:32296"
                     /translation="MALLTPQGVKEVFQLQRPQGRERLRRLLNWEEFDEQRDSRRSIL
                     LDTLYESIIFAVGKGFPWVEVAQVVKFTEELLRETKGCSITEAVTILGNKLRDYRGHF
                     NTTHLLALCDYFHHTFIRHYKLYQYVLGQDQQVDLTVAHLEVCMPPHPLPLAEGMDRD
                     LWIHEQQVATLTEAEAQKRADVLLLKEALRLERENSLQKAFAAAAPAQPGQVLERQEL
                     ESLICQAVHTQMELLQELLQRQIQNTFAILDLKLQKKTLNLNAPTPIPPPITSHAGQE
                     EALKPQRASKGKKAKARK"
     exon            87..279
                     /gene="C8orf74"
                     /inference="alignment:Splign:2.1.0"
     exon            280..686
                     /gene="C8orf74"
                     /inference="alignment:Splign:2.1.0"
     exon            687..1042
                     /gene="C8orf74"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1024..1029
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C8orf74"
                     /note="hexamer: ATTAAA"
     polyA_site      1042
                     /gene="C8orf74"
                     /note="major polyA site"
ORIGIN      
        1 agtctggctc cgtctcctgg caaccagatg caggggccat ggcactctta acaccccagg
       61 gagtgaaaga agtcttccaa cttcagagac cacaaggtcg ggagcgcctg cggaggcttc
      121 tgaactggga ggagtttgac gaacagagag actcccggag gagcatcctg ctggacaccc
      181 tctacgagag catcatcttt gcagtgggca aaggcttccc atgggtggag gtggcccagg
      241 tggtcaagtt cacagaagag ctgctaaggg aaaccaaagg ctgctccatt actgaggctg
      301 tgacgatcct ggggaacaag cttagagatt accggggcca tttcaacacc acccacctgc
      361 tggccctctg tgactacttc caccacacct tcatccgcca ctacaaactc taccagtatg
      421 tcctgggcca ggaccagcag gtcgacctga ccgttgccca cctggaggtg tgcatgccac
      481 cccatcccct cccgctggcc gagggcatgg acagggactt gtggatccac gagcagcagg
      541 tggccacact gacggaggcc gaggcacaga agcgcgccga cgtgctgctc ctgaaagagg
      601 cgctgcgcct ggagcgggag aactcgctgc agaaggcgtt cgctgccgcc gcgcctgcgc
      661 agcccggcca ggtcctggag agacaggagt tggagagcct catctgccag gcagtccaca
      721 cccagatgga gctcctgcag gagctgctgc agcgccagat ccagaacaca ttcgccatct
      781 tggacctgaa gcttcagaag aagactctga acctcaacgc ccccacccct atcccgcccc
      841 ccatcaccag ccacgcaggc caggaggaag ccctgaagcc ccaaagagcg agcaaaggaa
      901 agaaagcgaa ggcaaggaag tagaaggtcc cgactgccac acgagactga ctggggacca
      961 gccacccata accatgagcc ttgcggcacg gtgagctcag cacccacaga gagacttctt
     1021 gtgattaaaa gaaacaaacc ca
//
