LOCUS       NM_001204173            4519 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens C8orf44-SGK3 readthrough (C8orf44-SGK3), mRNA.
ACCESSION   NM_001204173
VERSION     NM_001204173.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Malik,N., Nirujogi,R.S., Peltier,J., Macartney,T., Wightman,M.,
            Prescott,A.R., Gourlay,R., Trost,M., Alessi,D.R. and Karapetsas,A.
  TITLE     Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase
            specifically phosphorylates endosomal proteins including
            Syntaxin-7, Syntaxin-12, RFIP4 and WDR44
  JOURNAL   Biochem J 476 (20), 3081-3107 (2019)
   PUBMED   31665227
  REMARK    GeneRIF: These results reveal novel substrates for SGK3 and suggest
            a mechanism by which STX7 and STX12 SNARE complexes are regulated
            by SGK3. They reveal new biomarkers for monitoring SGK3 pathway
            activity.
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Bruhn,M.A., Pearson,R.B., Hannan,R.D. and Sheppard,K.E.
  TITLE     Second AKT: the rise of SGK in cancer signalling
  JOURNAL   Growth Factors 28 (6), 394-408 (2010)
   PUBMED   20919962
  REMARK    Review article
REFERENCE   3  (bases 1 to 4519)
  AUTHORS   Prakash,T., Sharma,V.K., Adati,N., Ozawa,R., Kumar,N., Nishida,Y.,
            Fujikake,T., Takeda,T. and Taylor,T.D.
  TITLE     Expression of conjoined genes: another mechanism for gene
            regulation in eukaryotes
  JOURNAL   PLoS One 5 (10), e13284 (2010)
   PUBMED   20967262
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4519)
  AUTHORS   Greenman,C., Stephens,P., Smith,R., Dalgliesh,G.L., Hunter,C.,
            Bignell,G., Davies,H., Teague,J., Butler,A., Stevens,C., Edkins,S.,
            O'Meara,S., Vastrik,I., Schmidt,E.E., Avis,T., Barthorpe,S.,
            Bhamra,G., Buck,G., Choudhury,B., Clements,J., Cole,J., Dicks,E.,
            Forbes,S., Gray,K., Halliday,K., Harrison,R., Hills,K., Hinton,J.,
            Jenkinson,A., Jones,D., Menzies,A., Mironenko,T., Perry,J.,
            Raine,K., Richardson,D., Shepherd,R., Small,A., Tofts,C.,
            Varian,J., Webb,T., West,S., Widaa,S., Yates,A., Cahill,D.P.,
            Louis,D.N., Goldstraw,P., Nicholson,A.G., Brasseur,F.,
            Looijenga,L., Weber,B.L., Chiew,Y.E., DeFazio,A., Greaves,M.F.,
            Green,A.R., Campbell,P., Birney,E., Easton,D.F.,
            Chenevix-Trench,G., Tan,M.H., Khoo,S.K., Teh,B.T., Yuen,S.T.,
            Leung,S.Y., Wooster,R., Futreal,P.A. and Stratton,M.R.
  TITLE     Patterns of somatic mutation in human cancer genomes
  JOURNAL   Nature 446 (7132), 153-158 (2007)
   PUBMED   17344846
REFERENCE   5  (bases 1 to 4519)
  AUTHORS   Sjoblom,T., Jones,S., Wood,L.D., Parsons,D.W., Lin,J., Barber,T.D.,
            Mandelker,D., Leary,R.J., Ptak,J., Silliman,N., Szabo,S.,
            Buckhaults,P., Farrell,C., Meeh,P., Markowitz,S.D., Willis,J.,
            Dawson,D., Willson,J.K., Gazdar,A.F., Hartigan,J., Wu,L., Liu,C.,
            Parmigiani,G., Park,B.H., Bachman,K.E., Papadopoulos,N.,
            Vogelstein,B., Kinzler,K.W. and Velculescu,V.E.
  TITLE     The consensus coding sequences of human breast and colorectal
            cancers
  JOURNAL   Science 314 (5797), 268-274 (2006)
   PUBMED   16959974
REFERENCE   6  (bases 1 to 4519)
  AUTHORS   Tessier,M. and Woodgett,J.R.
  TITLE     Role of the Phox homology domain and phosphorylation in activation
            of serum and glucocorticoid-regulated kinase-3
  JOURNAL   J Biol Chem 281 (33), 23978-23989 (2006)
   PUBMED   16790420
REFERENCE   7  (bases 1 to 4519)
  AUTHORS   Loffing,J., Flores,S.Y. and Staub,O.
  TITLE     Sgk kinases and their role in epithelial transport
  JOURNAL   Annu Rev Physiol 68, 461-490 (2006)
   PUBMED   16460280
  REMARK    Review article
REFERENCE   8  (bases 1 to 4519)
  AUTHORS   Gamper,N., Fillon,S., Feng,Y., Friedrich,B., Lang,P.A., Henke,G.,
            Huber,S.M., Kobayashi,T., Cohen,P. and Lang,F.
  TITLE     K+ channel activation by all three isoforms of serum- and
            glucocorticoid-dependent protein kinase SGK
  JOURNAL   Pflugers Arch 445 (1), 60-66 (2002)
   PUBMED   12397388
REFERENCE   9  (bases 1 to 4519)
  AUTHORS   Dai,F., Yu,L., He,H., Zhao,Y., Yang,J., Zhang,X. and Zhao,S.
  TITLE     Cloning and mapping of a novel human serum/glucocorticoid regulated
            kinase-like gene, SGKL, to chromosome 8q12.3-q13.1
  JOURNAL   Genomics 62 (1), 95-97 (1999)
   PUBMED   10585774
REFERENCE   10 (bases 1 to 4519)
  AUTHORS   Kobayashi,T., Deak,M., Morrice,N. and Cohen,P.
  TITLE     Characterization of the structure and regulation of two novel
            isoforms of serum- and glucocorticoid-induced protein kinase
  JOURNAL   Biochem J 344 Pt 1 (Pt 1), 189-197 (1999)
   PUBMED   10548550
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC090154.19, BX649057.1 and AC011031.12.
            
            On Jun 2, 2019 this sequence version replaced NM_001204173.1.
            
            Summary: This locus represents naturally occurring read-through
            transcription between the neighboring putative uncharacterized
            protein C8orf44 (GeneID 56260) and serine/threonine-protein kinase
            Sgk3 (GeneID 23678) genes on chromosome 8. The read-through
            transcript produces a protein that shares sequence identity with
            the downstream gene product. [provided by RefSeq, Feb 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX649057.1, SRR1803614.255134.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142680, SAMEA2149178
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 23678, 56260
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                AC090154.19        49110-49129
            21-302              BX649057.1         2-283
            303-303             AC090154.19        61431-61431
            304-919             BX649057.1         285-900
            920-948             AC011031.12        68483-68511
            949-2963            BX649057.1         930-2944
            2964-4519           AC011031.12        106575-108130
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.1"
     gene            1..4519
                     /gene="C8orf44-SGK3"
                     /note="C8orf44-SGK3 readthrough"
                     /db_xref="GeneID:100533105"
                     /db_xref="HGNC:HGNC:48354"
     exon            1..106
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            107..410
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            411..466
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            467..683
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             588..2078
                     /gene="C8orf44-SGK3"
                     /EC_number="2.7.11.1"
                     /note="serum/glucocorticoid-regulated kinase 3;
                     cytokine-independent survival kinase"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Sgk3"
                     /protein_id="NP_001191102.1"
                     /db_xref="GeneID:100533105"
                     /db_xref="HGNC:HGNC:48354"
                     /translation="MQRDHTMDYKESCPSVSIPSSDEHREKKKRFTVYKVLVSVGRSE
                     WFVFRRYAEFDKLYNTLKKQFPAMALKIPAKRIFGDNFDPDFIKQRRAGLNEFIQNLV
                     RYPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSSQKLHSTSQNINLGPSGNPHAKPT
                     DFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHIMAERNVLLKNV
                     KHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGY
                     LHSIKIVYRDLKPENILLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIR
                     KQPYDNTVDWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSIL
                     EELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFD
                     TAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFL"
     exon            684..767
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            768..840
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            841..916
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1004
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1054
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1112
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1196
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1328
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1441
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1478
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1565
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1661
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1817
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1907
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..4519
                     /gene="C8orf44-SGK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4342..4347
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C8orf44-SGK3"
                     /note="hexamer: AATAAA"
     polyA_site      4364
                     /gene="C8orf44-SGK3"
                     /note="major polyA site"
     regulatory      4497..4502
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C8orf44-SGK3"
                     /note="hexamer: AATAAA"
     polyA_site      4519
                     /gene="C8orf44-SGK3"
                     /note="major polyA site"
ORIGIN      
        1 agggaagctt ggttaaaaac agttatggca gtgggagtcg aagcgagggt ctgaagttca
       61 cgactactag aaggggaggg gagtggaaag gctctcagtg aaaaaggtat tagaattatt
      121 tctgaattat cagtctctca tttgtgcttt ggagaagcag aaaaggcaaa aggggtcttt
      181 ggccatcttc tgctggagct tccagggagg atgtgtctcc aagagaccag atgtaccgag
      241 tttgaaatcc cagaagccca agaggaaaag aatcacaggg aggaaaagac tgtccaaagg
      301 cttctggagt cttctgttct ctaaccttgg aaggttttga acaatatttc tcagaggata
      361 gcctttcact tattcatctg tccagcatga ctcatccccg ggagtgttga atgtctcctg
      421 tcttcctcca gcattcacaa accaggaacg gctagctttt tgacctgttg catgatggaa
      481 tttgaacatt acttcaagag gttttgtatt ttggattagt taattgggtt tgtcctctgc
      541 tgactgtttc ttcggatgca ttttttggtg tgctcttgag ggattaaatg caaagagatc
      601 acaccatgga ctacaaggaa agctgcccaa gtgtaagcat tcccagctcc gatgaacaca
      661 gagagaaaaa gaagaggttt actgtttata aagttctggt ttcagtggga agaagtgaat
      721 ggtttgtctt caggagatat gcagagtttg ataaacttta taacacttta aaaaaacagt
      781 ttcctgctat ggccctgaag attcctgcca agagaatatt tggtgataat tttgatccag
      841 attttattaa acaaagacga gcaggactaa acgaattcat tcagaaccta gttaggtatc
      901 cagaacttta taaccatcca gatgtcagag cattccttca aatggacagt ccaaaacacc
      961 agtcagatcc atctgaagat gaggatgaaa gaagttctca gaagctacac tctacctcac
     1021 agaacatcaa cctgggaccg tctggaaatc ctcatgccaa accaactgac tttgatttct
     1081 taaaagttat tggaaaaggc agctttggca aggttcttct tgcaaaacgg aaactggatg
     1141 gaaaatttta tgctgtcaaa gtgttacaga aaaaaatagt tctcaacaga aaagagcaaa
     1201 aacatattat ggctgaacgt aatgtgctct tgaaaaatgt gaaacatccg tttttggttg
     1261 gattgcatta ttccttccaa acaactgaaa agctttattt tgttctggat tttgttaatg
     1321 gaggggagct ttttttccac ttacaaagag aacggtcctt tcctgagcac agagctaggt
     1381 tttacgctgc tgaaattgct agtgcattgg gttacttaca ttccatcaaa atagtataca
     1441 gagacttgaa accagaaaat attcttttgg attcagtagg acatgttgtc ttaacagatt
     1501 ttgggctttg taaagaagga attgctattt ctgacaccac taccacattt tgtgggacac
     1561 cagagtatct tgcacctgaa gtaattagaa aacagcccta tgacaatact gtagattggt
     1621 ggtgccttgg ggctgttctg tatgaaatgc tgtatggatt gcctcctttt tattgccgag
     1681 atgttgctga aatgtatgac aatatccttc acaaacccct aagtttgagg ccaggagtga
     1741 gtcttacagc ctggtccatt ctggaagaac tcctagaaaa agacaggcaa aatcgacttg
     1801 gtgccaagga agactttctt gaaattcaga atcatccttt ttttgaatca ctcagctggg
     1861 ctgaccttgt acaaaagaag attccaccac catttaatcc taatgtggct ggaccagatg
     1921 atatcagaaa ctttgacaca gcatttacag aagaaacagt tccatattct gtgtgtgtat
     1981 cttctgacta ttctatagtg aatgccagtg tattggaggc agatgatgca ttcgttggtt
     2041 tctcttatgc acctccttca gaagacttat ttttgtgagc agtttgccat tcagaaacca
     2101 ttgagcaaaa taagtctata gatgggactg aaacttctat ttgtgtgaat atattcaaat
     2161 atgtataact agtgcctcat ttttatatgt aatgatgaaa actatgaaaa aatgtatttt
     2221 cttctatgtg caagaaaaat agggcatttc aaagagctgt tttgattaaa atttatattc
     2281 ttgtttaata agcttatttt taaacaattt aaaagctatt attcttagca ttaacctatt
     2341 tttaaagaaa ccttttttgc tattgactgt tttttccctc taagtttaca ctaacatcta
     2401 cccaagatag actgtttttt aacagtcaat ttcagttcag ctaacatata ttaatacctt
     2461 tgtaactctt tgctatggct tttgttatca caccaaaact atgcaattgg tacatggttg
     2521 tttaagaaga aaccgtattt ttccatgata aatcactgtt tgaaatattt ggttcatggt
     2581 atgatcgaaa tgtaaaagca taattaacac attggctgct agttaacaat tggaataact
     2641 ttattctgca gatcatttaa gaagtaacag gccgggcgcg gtggctcacg cctgtaatcc
     2701 cagcactttg ggaggctgag gcgggcagat cacctgaggt caggagttgg agaccagcct
     2761 gaccaacatg gacaaacccc gtctctacta aaaatacaaa attggcaggg tgtggtggca
     2821 catgcctata atcccagcta cttgggaggc taaggcagga gaatcgcttg aacccgggag
     2881 gcggaggttg cagtgagccg agatcgcacc attgcactcc tgcctgggca acaagagtga
     2941 aactccatct ccaaaaaaaa aaagaaaaag taacaaaagg aaattatttg tttttgaaat
     3001 accagttcaa ctttgtggat tatttttcct ctgaaggaaa agaaaaggct taatggttag
     3061 gatttttaag tattcccaaa gatctgaagg gtaataaaat gtactggatt ttttaaggtg
     3121 gtaccaaaaa tgaatgtctg tcatatattt atattacaaa tacattatat ttatgttcta
     3181 ttcatctttt gaatgtttag tatgctatta agtcattctg aatctttgta tttgcttttg
     3241 caaataggta tttcaaagct cttttcctaa ctggttaagt aaaataaaaa attgagcttt
     3301 ctagaatatt tgcctaattg ggaattaaaa agtaaaataa taggccaggc atggtggctc
     3361 atgcctataa gcaccctggg aggccgaggc aggcagatta tttgagctca ggagtttgag
     3421 accatcctgg gcaacatggc gaaaccctat ctctacaaaa aatacaaaaa ttagccagac
     3481 atggtggcac atgcctttag tcccagctac tctggaggct gaagttggag gatggcttga
     3541 gcccacgaga tggaagttgc agtgaactga aattgtgcca ctgcactttt cagcctgggt
     3601 gccacagcga gaccctagtt aagagaaaaa aaaagtaaaa acaaattgtg ggtcaaagta
     3661 aatgtataca gttttattac aatgtaacaa aagttgaaaa tcaggcagat gtgtattcag
     3721 tatccaattc aatatatctt agaaaaagca caggaaacag accttaaaat tgtaacctac
     3781 caactaactt acatgcttat aaaagtaaag gagaataact ggccgggcac ggtggctcat
     3841 gcctataaaa ttccagcact ttgggaggcc aaggcaggag gatcacttga gcctatgagt
     3901 tcaagaccag cctaggcaat gtagtgacac ctcatttcta tttattttaa aaaaaagaga
     3961 gagtaactac agaagaactt taaaaaataa aaaataagct taccttggat tcttggctta
     4021 gagtagaggt tttttttaag ttatggagga aacatttttg taaaagttta atgacccact
     4081 ttagatgctc caagaacaag catcccttcc atgtatgtct tgagaaagaa atcacagaag
     4141 catttctcac caatactctt tggcttaaaa tgttcagcag aattgggcag tgggggtgac
     4201 ttttcttata ttaataatat ttacatccaa tacactgaat cttcctttag aggtaagact
     4261 ttaatatcta tactgtaaat atttggttta tttggcacta ctgtaagttt tgtttttcac
     4321 aaagctctta ttatgaagca aaataaaaat tctagtttct tgtatgattt tttgtactca
     4381 ttcattcctg ttaagctgcc aaaaattaaa gtgcaatatt gtatattttt aagaacaaat
     4441 ttaaaataga attttgatgt ttctcagatc acaagaaata caaatctata tagtataata
     4501 aaatcagcaa aaagatcaa
//
