LOCUS       NM_000606                881 bp    mRNA    linear   PRI 05-JUN-2024
DEFINITION  Homo sapiens complement C8 gamma chain (C8G), mRNA.
ACCESSION   NM_000606
VERSION     NM_000606.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Yang,Y., Shen,T., Li,M., Leng,B., Yao,R., Gao,Y., Sun,H., Li,Z. and
            Zhang,J.
  TITLE     Elevated complement component 8 gamma levels in astrocyte-derived
            exosomes are associated with cognitive impairment in obstructive
            sleep apnea patients without dementia
  JOURNAL   Neurosci Lett 794, 137010 (2023)
   PUBMED   36509166
  REMARK    GeneRIF: Elevated complement component 8 gamma levels in
            astrocyte-derived exosomes are associated with cognitive impairment
            in obstructive sleep apnea patients without dementia.
REFERENCE   2  (bases 1 to 881)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 881)
  AUTHORS   Menny,A., Serna,M., Boyd,C.M., Gardner,S., Joseph,A.P.,
            Morgan,B.P., Topf,M., Brooks,N.J. and Bubeck,D.
  TITLE     CryoEM reveals how the complement membrane attack complex ruptures
            lipid bilayers
  JOURNAL   Nat Commun 9 (1), 5316 (2018)
   PUBMED   30552328
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 881)
  AUTHORS   Franc,V., Zhu,J. and Heck,A.J.R.
  TITLE     Comprehensive Proteoform Characterization of Plasma Complement
            Component C8alphabetagamma by Hybrid Mass Spectrometry Approaches
  JOURNAL   J Am Soc Mass Spectrom 29 (6), 1099-1110 (2018)
   PUBMED   29532326
  REMARK    GeneRIF: These data provide a detailed specification of
            co-occurring C8 proteoforms, including experimental evidence on
            N-glycosylation, C-mannosylation, and O-glycosylation.
REFERENCE   5  (bases 1 to 881)
  AUTHORS   Bastos-Amador,P., Royo,F., Gonzalez,E., Conde-Vancells,J.,
            Palomo-Diez,L., Borras,F.E. and Falcon-Perez,J.M.
  TITLE     Proteomic analysis of microvesicles from plasma of healthy donors
            reveals high individual variability
  JOURNAL   J Proteomics 75 (12), 3574-3584 (2012)
   PUBMED   22516433
REFERENCE   6  (bases 1 to 881)
  AUTHORS   Haefliger,J.A., Peitsch,M.C., Jenne,D.E. and Tschopp,J.
  TITLE     Structural and functional characterization of complement C8 gamma,
            a member of the lipocalin protein family
  JOURNAL   Mol Immunol 28 (1-2), 123-131 (1991)
   PUBMED   1707134
REFERENCE   7  (bases 1 to 881)
  AUTHORS   Kaufman,K.M., Snider,J.V., Spurr,N.K., Schwartz,C.E. and
            Sodetz,J.M.
  TITLE     Chromosomal assignment of genes encoding the alpha, beta, and gamma
            subunits of human complement protein C8: identification of a close
            physical linkage between the alpha and the beta loci
  JOURNAL   Genomics 5 (3), 475-480 (1989)
   PUBMED   2613233
REFERENCE   8  (bases 1 to 881)
  AUTHORS   Muller-Eberhard,H.J.
  TITLE     Molecular organization and function of the complement system
  JOURNAL   Annu Rev Biochem 57, 321-347 (1988)
   PUBMED   3052276
  REMARK    Review article
REFERENCE   9  (bases 1 to 881)
  AUTHORS   Haefliger,J.A., Jenne,D., Stanley,K.K. and Tschopp,J.
  TITLE     Structural homology of human complement component C8 gamma and
            plasma protein HC: identity of the cysteine bond pattern
  JOURNAL   Biochem Biophys Res Commun 149 (2), 750-754 (1987)
   PUBMED   2447883
REFERENCE   10 (bases 1 to 881)
  AUTHORS   Hunt,L.T., Elzanowski,A. and Barker,W.C.
  TITLE     The homology of complement factor C8 gamma chain and
            alpha-1-microglobulin
  JOURNAL   Biochem Biophys Res Commun 149 (1), 282-288 (1987)
   PUBMED   2446620
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY027599.1, X06465.1,
            BX282392.1 and GD261753.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 11, 2018 this sequence version replaced NM_000606.2.
            
            Summary: The protein encoded by this gene belongs to the lipocalin
            family. It is one of the three subunits that constitutes complement
            component 8 (C8), which is composed of a disulfide-linked C8
            alpha-gamma heterodimer and a non-covalently associated C8 beta
            chain. C8 participates in the formation of the membrane attack
            complex (MAC) on bacterial cell membranes. While subunits alpha and
            beta play a role in complement-mediated bacterial killing, the
            gamma subunit is not required for the bactericidal activity.
            [provided by RefSeq, Jul 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189664.154141.1,
                                           SRR5189664.119234.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371634.7/ ENSP00000360697.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                HY027599.1         59-110
            53-430              X06465.1           36-413
            431-755             BX282392.1         166-490
            756-881             GD261753.1         260-385
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.3"
     gene            1..881
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /note="complement C8 gamma chain"
                     /db_xref="GeneID:733"
                     /db_xref="HGNC:HGNC:1354"
                     /db_xref="MIM:120930"
     exon            1..216
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..687
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /note="complement component C8 gamma chain; complement
                     component 8, gamma polypeptide"
                     /codon_start=1
                     /product="complement component C8 gamma chain precursor"
                     /protein_id="NP_000597.2"
                     /db_xref="CCDS:CCDS7017.1"
                     /db_xref="GeneID:733"
                     /db_xref="HGNC:HGNC:1354"
                     /db_xref="MIM:120930"
                     /translation="MLPPGTATLLTLLLAAGSLGQKPQRPRRPASPISTIQPKANFDA
                     QQFAGTWLLVAVGSACRFLQEQGHRAEATTLHVAPQGTAMAVSTFRKLDGICWQVRQL
                     YGDTGVLGRFLLQARDARGAVHVVVAETDYQSFAVLYLERAGQLSVKLYARSLPVSDS
                     VLSGFEQRVQEAHLTEDQIFYFPKYGFCEAADQFHVLDEVRR"
     exon            217..353
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /inference="alignment:Splign:2.1.0"
     exon            354..424
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /inference="alignment:Splign:2.1.0"
     exon            425..532
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /inference="alignment:Splign:2.1.0"
     exon            533..634
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /inference="alignment:Splign:2.1.0"
     exon            635..673
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /inference="alignment:Splign:2.1.0"
     exon            674..881
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      862..867
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /note="hexamer: ATTAAA"
     polyA_site      880
                     /gene="C8G"
                     /gene_synonym="C8C"
                     /note="major polyA site"
ORIGIN      
        1 agcagagtag actctgtcct gggacttggt ggtgctaccc ttggcctccc acagtcctgc
       61 caccctgctg ccgccaccat gctgccccct gggactgcga ccctcttgac tctgctcctg
      121 gcagctggct cgctgggcca gaagcctcag aggccacgcc ggcccgcatc ccccatcagc
      181 accatccagc ccaaggccaa ttttgatgct cagcagtttg cagggacctg gctccttgtg
      241 gctgtgggct ccgcttgccg tttcctgcag gagcagggcc accgggccga ggccaccaca
      301 ctgcatgtgg ctccccaggg cacagccatg gctgtcagta ccttccgaaa gctggatggg
      361 atctgctggc aggtgcgcca gctctatgga gacacagggg tcctcggccg cttcctgctt
      421 caagcccgag acgcccgagg ggctgtgcac gtggttgtcg ctgagaccga ctaccagagt
      481 ttcgctgtcc tgtacctgga gcgggcgggg cagctgtcag tgaagctcta cgcccgctcg
      541 ctccctgtga gcgactcggt cctgagtggg tttgagcagc gggtccagga ggcccacctg
      601 actgaggacc agatcttcta cttccccaag tacggcttct gcgaggctgc agaccagttc
      661 cacgtcctgg acgaagtgag gaggtgaggc cggcacacag ctccagtgct gagaagtcag
      721 tgccccgaga gacgacccca ccagtggggt gcccgctgcc tgtcctccgt gaaaccagcc
      781 tcagatcagg gccctgccac ccagggcagg ggatcttctg ccggctgccc cagaggacag
      841 tgggtggagt ggtacctact tattaaatgt ctcagacccc t
//
