LOCUS       NM_001735               5464 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens complement C5 (C5), transcript variant 1, mRNA.
ACCESSION   NM_001735
VERSION     NM_001735.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Rech,L., Dietrich-Ntoukas,T., Reinach,P.S., Brockmann,T., Pleyer,U.
            and Mergler,S.
  TITLE     Complement Component C5a and Fungal Pathogen Induce Diverse
            Responses through Crosstalk between Transient Receptor Potential
            Channel (TRPs) Subtypes in Human Conjunctival Epithelial Cells
  JOURNAL   Cells 13 (16), 1329 (2024)
   PUBMED   39195219
  REMARK    GeneRIF: Complement Component C5a and Fungal Pathogen Induce
            Diverse Responses through Crosstalk between Transient Receptor
            Potential Channel (TRPs) Subtypes in Human Conjunctival Epithelial
            Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Chou,Y.L., Hsu,Y.A., Lin,C.F., Chen,C.S., Tien,P.T., Wang,Y.C.,
            Chang,C.Y., Lin,E.S., Chen,J.J., Wu,M.Y., Chuang,C.Y., Lin,H.J. and
            Wan,L.
  TITLE     Complement decay-accelerating factor inhibits inflammation-induced
            myopia development
  JOURNAL   Mol Immunol 171, 47-55 (2024)
   PUBMED   38795684
  REMARK    GeneRIF: Complement decay-accelerating factor inhibits
            inflammation-induced myopia development.
REFERENCE   3  (bases 1 to 5464)
  AUTHORS   Yu,M., Yang,Y., Zhang,J., Liu,R., Huang,L., Wu,J., Kang,Z., Zhou,J.
            and Yang,Z.
  TITLE     Association between TRAF1/C5 Gene Polymorphisms and IgA Vasculitis
            in Chinese Children
  JOURNAL   Immunol Invest 53 (2), 281-293 (2024)
   PUBMED   38117213
  REMARK    GeneRIF: Association between TRAF1/C5 Gene Polymorphisms and IgA
            Vasculitis in Chinese Children.
REFERENCE   4  (bases 1 to 5464)
  AUTHORS   Liu,X., Hu,Y., Yu,X., Tan,Y., Yu,F., Chen,M. and Zhao,M.
  TITLE     Differential contributions of the C5b-9 and C5a/C5aR pathways to
            microvascular and macrovascular thrombosis in complement-mediated
            thrombotic microangiopathy patients
  JOURNAL   Clin Immunol 259, 109871 (2024)
   PUBMED   38101498
  REMARK    GeneRIF: Differential contributions of the C5b-9 and C5a/C5aR
            pathways to microvascular and macrovascular thrombosis in
            complement-mediated thrombotic microangiopathy patients.
REFERENCE   5  (bases 1 to 5464)
  AUTHORS   Merle,N.S., Church,S.E., Fremeaux-Bacchi,V. and Roumenina,L.T.
  TITLE     Complement System Part I - Molecular Mechanisms of Activation and
            Regulation
  JOURNAL   Front Immunol 6, 262 (2015)
   PUBMED   26082779
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5464)
  AUTHORS   DiScipio,R.G.
  TITLE     Formation and structure of the C5b-7 complex of the lytic pathway
            of complement
  JOURNAL   J Biol Chem 267 (24), 17087-17094 (1992)
   PUBMED   1387399
REFERENCE   7  (bases 1 to 5464)
  AUTHORS   Gerard,N.P. and Gerard,C.
  TITLE     The chemotactic receptor for human C5a anaphylatoxin
  JOURNAL   Nature 349 (6310), 614-617 (1991)
   PUBMED   1847994
REFERENCE   8  (bases 1 to 5464)
  AUTHORS   Haviland,D.L., Haviland,J.C., Fleischer,D.T., Hunt,A. and
            Wetsel,R.A.
  TITLE     Complete cDNA sequence of human complement pro-C5. Evidence of
            truncated transcripts derived from a single copy gene
  JOURNAL   J Immunol 146 (1), 362-368 (1991)
   PUBMED   1984448
REFERENCE   9  (bases 1 to 5464)
  AUTHORS   Tack,B.F., Morris,S.C. and Prahl,J.W.
  TITLE     Fifth component of human complement: purification from plasma and
            polypeptide chain structure
  JOURNAL   Biochemistry 18 (8), 1490-1497 (1979)
   PUBMED   106884
REFERENCE   10 (bases 1 to 5464)
  AUTHORS   Fernandez,H.N. and Hugli,T.E.
  TITLE     Primary structural analysis of the polypeptide portion of human C5a
            anaphylatoxin. Polypeptide sequence determination and assignment of
            the oligosaccharide attachment site in C5a
  JOURNAL   J Biol Chem 253 (19), 6955-6964 (1978)
   PUBMED   690134
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC022299.1, M57729.1,
            AV682721.1, M65134.1, T82068.1 and BG533927.1.
            
            On Aug 31, 2019 this sequence version replaced NM_001735.2.
            
            Summary: This gene encodes a component of the complement system, a
            part of the innate immune system that plays an important role in
            inflammation, host homeostasis, and host defense against pathogens.
            The encoded preproprotein is proteolytically processed to generate
            multiple protein products, including the C5 alpha chain, C5 beta
            chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of
            the C5 alpha and beta chains, which are linked by a disulfide
            bridge. Cleavage of the alpha chain by a convertase enzyme results
            in the formation of the C5a anaphylatoxin, which possesses potent
            spasmogenic and chemotactic activity, and the C5b macromolecular
            cleavage product, a subunit of the membrane attack complex (MAC).
            Mutations in this gene cause complement component 5 deficiency, a
            disease characterized by recurrent bacterial infections.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at an
            alternate start site compared to variant 2. The encoded isoform (1)
            has a shorter and unique N-terminus compared to isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M57729.1, BC113740.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000223642.3/ ENSP00000223642.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                BC022299.1         3-49
            48-514              M57729.1           31-497
            515-706             BC022299.1         517-708
            707-1194            M57729.1           690-1177
            1195-1660           BC022299.1         1197-1662
            1661-1745           AV682721.1         122-206
            1746-2432           M65134.1           484-1170
            2433-2451           T82068.1           150-168
            2452-5461           M65134.1           1190-4199
            5462-5464           BG533927.1         856-858
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.2"
     gene            1..5464
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /note="complement C5"
                     /db_xref="GeneID:727"
                     /db_xref="HGNC:HGNC:1331"
                     /db_xref="MIM:120900"
     exon            1..94
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..5060
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; anaphylatoxin C5a analog; complement component
                     5; C3 and PZP-like alpha-2-macroglobulin domain-containing
                     protein 4; prepro-C5; C5a anaphylatoxin"
                     /codon_start=1
                     /product="complement C5 isoform 1 preproprotein"
                     /protein_id="NP_001726.2"
                     /db_xref="CCDS:CCDS6826.1"
                     /db_xref="GeneID:727"
                     /db_xref="HGNC:HGNC:1331"
                     /db_xref="MIM:120900"
                     /translation="MGLLGILCFLIFLGKTWGQEQTYVISAPKIFRVGASENIVIQVY
                     GYTEAFDATISIKSYPDKKFSYSSGHVHLSSENKFQNSAILTIQPKQLPGGQNPVSYV
                     YLEVVSKHFSKSKRMPITYDNGFLFIHTDKPVYTPDQSVKVRVYSLNDDLKPAKRETV
                     LTFIDPEGSEVDMVEEIDHIGIISFPDFKIPSNPRYGMWTIKAKYKEDFSTTGTAYFE
                     VKEYVLPHFSVSIEPEYNFIGYKNFKNFEITIKARYFYNKVVTEADVYITFGIREDLK
                     DDQKEMMQTAMQNTMLINGIAQVTFDSETAVKELSYYSLEDLNNKYLYIAVTVIESTG
                     GFSEEAEIPGIKYVLSPYKLNLVATPLFLKPGIPYPIKVQVKDSLDQLVGGVPVTLNA
                     QTIDVNQETSDLDPSKSVTRVDDGVASFVLNLPSGVTVLEFNVKTDAPDLPEENQARE
                     GYRAIAYSSLSQSYLYIDWTDNHKALLVGEHLNIIVTPKSPYIDKITHYNYLILSKGK
                     IIHFGTREKFSDASYQSINIPVTQNMVPSSRLLVYYIVTGEQTAELVSDSVWLNIEEK
                     CGNQLQVHLSPDADAYSPGQTVSLNMATGMDSWVALAAVDSAVYGVQRGAKKPLERVF
                     QFLEKSDLGCGAGGGLNNANVFHLAGLTFLTNANADDSQENDEPCKEILRPRRTLQKK
                     IEEIAAKYKHSVVKKCCYDGACVNNDETCEQRAARISLGPRCIKAFTECCVVASQLRA
                     NISHKDMQLGRLHMKTLLPVSKPEIRSYFPESWLWEVHLVPRRKQLQFALPDSLTTWE
                     IQGVGISNTGICVADTVKAKVFKDVFLEMNIPYSVVRGEQIQLKGTVYNYRTSGMQFC
                     VKMSAVEGICTSESPVIDHQGTKSSKCVRQKVEGSSSHLVTFTVLPLEIGLHNINFSL
                     ETWFGKEILVKTLRVVPEGVKRESYSGVTLDPRGIYGTISRRKEFPYRIPLDLVPKTE
                     IKRILSVKGLLVGEILSAVLSQEGINILTHLPKGSAEAELMSVVPVFYVFHYLETGNH
                     WNIFHSDPLIEKQKLKKKLKEGMLSIMSYRNADYSYSVWKGGSASTWLTAFALRVLGQ
                     VNKYVEQNQNSICNSLLWLVENYQLDNGSFKENSQYQPIKLQGTLPVEARENSLYLTA
                     FTVIGIRKAFDICPLVKIDTALIKADNFLLENTLPAQSTFTLAISAYALSLGDKTHPQ
                     FRSIVSALKREALVKGNPPIYRFWKDNLQHKDSSVPNTGTARMVETTAYALLTSLNLK
                     DINYVNPVIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLRLSMDIDVSYKHK
                     GALHNYKMTDKNFLGRPVEVLLNDDLIVSTGFGSGLATVHVTTVVHKTSTSEEVCSFY
                     LKIDTQDIEASHYRGYGNSDYKRIVACASYKPSREESSSGSSHAVMDISLPTGISANE
                     EDLKALVEGVDQLFTDYQIKDGHVILQLNSIPSSDFLCVRFRIFELFEVGFLSPATFT
                     VYEYHRPDKQCTMFYSTSNIKIQKVCEGAACKCVEADCGQMQEELDLTISAETRKQTA
                     CKPEIAYAYKVSITSITVENVFVKYKATLLDIYKTGEAVAEKDSEITFIKKVTCTNAE
                     LVKGRQYLIMGKEALQIKYNFSFRYIYPLDSLTWIEYWPRDTTCSSCQAFLANLDEFA
                     EDIFLNGC"
     exon            95..287
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            288..450
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            451..521
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            522..613
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            614..696
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            697..787
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            788..902
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1029
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1145
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1331
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1535
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1745
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1895
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2025
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2088
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2286
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2377
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2451
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2591
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2592..2819
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2880
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2881..2970
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2971..3183
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3259
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3260..3419
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3515
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3516..3687
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3688..3893
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3894..4046
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4047..4109
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4110..4191
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4192..4249
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4250..4352
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4353..4427
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4428..4533
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4534..4617
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4618..4707
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4708..4791
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4792..4930
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4931..5464
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5440..5445
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /note="hexamer: ATTAAA"
     polyA_site      5464
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /note="major polyA site"
ORIGIN      
        1 atatccgtgg tttcctgcta cctccaacca tgggcctttt gggaatactt tgttttttaa
       61 tcttcctggg gaaaacctgg ggacaggagc aaacatatgt catttcagca ccaaaaatat
      121 tccgtgttgg agcatctgaa aatattgtga ttcaagttta tggatacact gaagcatttg
      181 atgcaacaat ctctattaaa agttatcctg ataaaaaatt tagttactcc tcaggccatg
      241 ttcatttatc ctcagagaat aaattccaaa actctgcaat cttaacaata caaccaaaac
      301 aattgcctgg aggacaaaac ccagtttctt atgtgtattt ggaagttgta tcaaagcatt
      361 tttcaaaatc aaaaagaatg ccaataacct atgacaatgg atttctcttc attcatacag
      421 acaaacctgt ttatactcca gaccagtcag taaaagttag agtttattcg ttgaatgacg
      481 acttgaagcc agccaaaaga gaaactgtct taactttcat agatcctgaa ggatcagaag
      541 ttgacatggt agaagaaatt gatcatattg gaattatctc ttttcctgac ttcaagattc
      601 cgtctaatcc tagatatggt atgtggacga tcaaggctaa atataaagag gacttttcaa
      661 caactggaac cgcatatttt gaagttaaag aatatgtctt gccacatttt tctgtctcaa
      721 tcgagccaga atataatttc attggttaca agaactttaa gaattttgaa attactataa
      781 aagcaagata tttttataat aaagtagtca ctgaggctga cgtttatatc acatttggaa
      841 taagagaaga cttaaaagat gatcaaaaag aaatgatgca aacagcaatg caaaacacaa
      901 tgttgataaa tggaattgct caagtcacat ttgattctga aacagcagtc aaagaactgt
      961 catactacag tttagaagat ttaaacaaca agtaccttta tattgctgta acagtcatag
     1021 agtctacagg tggattttct gaagaggcag aaatacctgg catcaaatat gtcctctctc
     1081 cctacaaact gaatttggtt gctactcctc ttttcctgaa gcctgggatt ccatatccca
     1141 tcaaggtgca ggttaaagat tcgcttgacc agttggtagg aggagtccca gtaacactga
     1201 atgcacaaac aattgatgta aaccaagaga catctgactt ggatccaagc aaaagtgtaa
     1261 cacgtgttga tgatggagta gcttcctttg tgcttaatct cccatctgga gtgacggtgc
     1321 tggagtttaa tgtcaaaact gatgctccag atcttccaga agaaaatcag gccagggaag
     1381 gttaccgagc aatagcatac tcatctctca gccaaagtta cctttatatt gattggactg
     1441 ataaccataa ggctttgcta gtgggagaac atctgaatat tattgttacc cccaaaagcc
     1501 catatattga caaaataact cactataatt acttgatttt atccaagggc aaaattatcc
     1561 actttggcac gagggagaaa ttttcagatg catcttatca aagtataaac attccagtaa
     1621 cacagaacat ggttccttca tcccgacttc tggtctatta catcgtcaca ggagaacaga
     1681 cagcagaatt agtgtctgat tcagtctggt taaatattga agaaaaatgt ggcaaccagc
     1741 tccaggttca tctgtctcct gatgcagatg catattctcc aggccaaact gtgtctctta
     1801 atatggcaac tggaatggat tcctgggtgg cattagcagc agtggacagt gctgtgtatg
     1861 gagtccaaag aggagccaaa aagcccttgg aaagagtatt tcaattctta gagaagagtg
     1921 atctgggctg tggggcaggt ggtggcctca acaatgccaa tgtgttccac ctagctggac
     1981 ttaccttcct cactaatgca aatgcagatg actcccaaga aaatgatgaa ccttgtaaag
     2041 aaattctcag gccaagaaga acgctgcaaa agaagataga agaaatagct gctaaatata
     2101 aacattcagt agtgaagaaa tgttgttacg atggagcctg cgttaataat gatgaaacct
     2161 gtgagcagcg agctgcacgg attagtttag ggccaagatg catcaaagct ttcactgaat
     2221 gttgtgtcgt cgcaagccag ctccgtgcta atatctctca taaagacatg caattgggaa
     2281 ggctacacat gaagaccctg ttaccagtaa gcaagccaga aattcggagt tattttccag
     2341 aaagctggtt gtgggaagtt catcttgttc ccagaagaaa acagttgcag tttgccctac
     2401 ctgattctct aaccacctgg gaaattcaag gcgttggcat ttcaaacact ggtatatgtg
     2461 ttgctgatac tgtcaaggca aaggtgttca aagatgtctt cctggaaatg aatataccat
     2521 attctgttgt acgaggagaa cagatccaat tgaaaggaac tgtttacaac tataggactt
     2581 ctgggatgca gttctgtgtt aaaatgtctg ctgtggaggg aatctgcact tcggaaagcc
     2641 cagtcattga tcatcagggc acaaagtcct ccaaatgtgt gcgccagaaa gtagagggct
     2701 cctccagtca cttggtgaca ttcactgtgc ttcctctgga aattggcctt cacaacatca
     2761 atttttcact ggagacttgg tttggaaaag aaatcttagt aaaaacatta cgagtggtgc
     2821 cagaaggtgt caaaagggaa agctattctg gtgttacttt ggatcctagg ggtatttatg
     2881 gtaccattag cagacgaaag gagttcccat acaggatacc cttagatttg gtccccaaaa
     2941 cagaaatcaa aaggattttg agtgtaaaag gactgcttgt aggtgagatc ttgtctgcag
     3001 ttctaagtca ggaaggcatc aatatcctaa cccacctccc caaagggagt gcagaggcgg
     3061 agctgatgag cgttgtccca gtattctatg tttttcacta cctggaaaca ggaaatcatt
     3121 ggaacatttt tcattctgac ccattaattg aaaagcagaa actgaagaaa aaattaaaag
     3181 aagggatgtt gagcattatg tcctacagaa atgctgacta ctcttacagt gtgtggaagg
     3241 gtggaagtgc tagcacttgg ttaacagctt ttgctttaag agtacttgga caagtaaata
     3301 aatacgtaga gcagaaccaa aattcaattt gtaattcttt attgtggcta gttgagaatt
     3361 atcaattaga taatggatct ttcaaggaaa attcacagta tcaaccaata aaattacagg
     3421 gtaccttgcc tgttgaagcc cgagagaaca gcttatatct tacagccttt actgtgattg
     3481 gaattagaaa ggctttcgat atatgccccc tggtgaaaat cgacacagct ctaattaaag
     3541 ctgacaactt tctgcttgaa aatacactgc cagcccagag cacctttaca ttggccattt
     3601 ctgcgtatgc tctttccctg ggagataaaa ctcacccaca gtttcgttca attgtttcag
     3661 ctttgaagag agaagctttg gttaaaggta atccacccat ttatcgtttt tggaaagaca
     3721 atcttcagca taaagacagc tctgtaccta acactggtac ggcacgtatg gtagaaacaa
     3781 ctgcctatgc tttactcacc agtctgaact tgaaagatat aaattatgtt aacccagtca
     3841 tcaaatggct atcagaagag cagaggtatg gaggtggctt ttattcaacc caggacacaa
     3901 tcaatgccat tgagggcctg acggaatatt cactcctggt taaacaactc cgcttgagta
     3961 tggacatcga tgtttcttac aagcataaag gtgccttaca taattataaa atgacagaca
     4021 agaatttcct tgggaggcca gtagaggtgc ttctcaatga tgacctcatt gtcagtacag
     4081 gatttggcag tggcttggct acagtacatg taacaactgt agttcacaaa accagtacct
     4141 ctgaggaagt ttgcagcttt tatttgaaaa tcgatactca ggatattgaa gcatcccact
     4201 acagaggcta cggaaactct gattacaaac gcatagtagc atgtgccagc tacaagccca
     4261 gcagggaaga atcatcatct ggatcctctc atgcggtgat ggacatctcc ttgcctactg
     4321 gaatcagtgc aaatgaagaa gacttaaaag cccttgtgga aggggtggat caactattca
     4381 ctgattacca aatcaaagat ggacatgtta ttctgcaact gaattcgatt ccctccagtg
     4441 atttcctttg tgtacgattc cggatatttg aactctttga agttgggttt ctcagtcctg
     4501 ccactttcac agtgtacgaa taccacagac cagataaaca gtgtaccatg ttttatagca
     4561 cttccaatat caaaattcag aaagtctgtg aaggagccgc gtgcaagtgt gtagaagctg
     4621 attgtgggca aatgcaggaa gaattggatc tgacaatctc tgcagagaca agaaaacaaa
     4681 cagcatgtaa accagagatt gcatatgctt ataaagttag catcacatcc atcactgtag
     4741 aaaatgtttt tgtcaagtac aaggcaaccc ttctggatat ctacaaaact ggggaagctg
     4801 ttgctgagaa agactctgag attaccttca ttaaaaaggt aacctgtact aacgctgagc
     4861 tggtaaaagg aagacagtac ttaattatgg gtaaagaagc cctccagata aaatacaatt
     4921 tcagtttcag gtacatctac cctttagatt ccttgacctg gattgaatac tggcctagag
     4981 acacaacatg ttcatcgtgt caagcatttt tagctaattt agatgaattt gccgaagata
     5041 tctttttaaa tggatgctaa aattcctgaa gttcagctgc atacagtttg cacttatgga
     5101 ctcctgttgt tgaagttcgt ttttttgttt tcttcttttt ttaaacattc atagctggtc
     5161 ttatttgtaa agctcacttt acttagaatt agtggcactt gcttttatta gagaatgatt
     5221 tcaaatgctg taactttctg aaataacatg gccttggagg gcatgaagac agatactcct
     5281 ccaaggttat tggacaccgg aaacaataaa ttggaacacc tcctcaaacc taccactcag
     5341 gaatgtttgc tggggccgaa agaacagtcc attgaaaggg agtattacaa aaacatggcc
     5401 tttgcttgaa agaaaatacc aaggaacagg aaactgatca ttaaagcctg agtttgcttt
     5461 caaa
//
