LOCUS       NM_001317163            5482 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens complement C5 (C5), transcript variant 2, mRNA.
ACCESSION   NM_001317163
VERSION     NM_001317163.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Rech,L., Dietrich-Ntoukas,T., Reinach,P.S., Brockmann,T., Pleyer,U.
            and Mergler,S.
  TITLE     Complement Component C5a and Fungal Pathogen Induce Diverse
            Responses through Crosstalk between Transient Receptor Potential
            Channel (TRPs) Subtypes in Human Conjunctival Epithelial Cells
  JOURNAL   Cells 13 (16), 1329 (2024)
   PUBMED   39195219
  REMARK    GeneRIF: Complement Component C5a and Fungal Pathogen Induce
            Diverse Responses through Crosstalk between Transient Receptor
            Potential Channel (TRPs) Subtypes in Human Conjunctival Epithelial
            Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Chou,Y.L., Hsu,Y.A., Lin,C.F., Chen,C.S., Tien,P.T., Wang,Y.C.,
            Chang,C.Y., Lin,E.S., Chen,J.J., Wu,M.Y., Chuang,C.Y., Lin,H.J. and
            Wan,L.
  TITLE     Complement decay-accelerating factor inhibits inflammation-induced
            myopia development
  JOURNAL   Mol Immunol 171, 47-55 (2024)
   PUBMED   38795684
  REMARK    GeneRIF: Complement decay-accelerating factor inhibits
            inflammation-induced myopia development.
REFERENCE   3  (bases 1 to 5482)
  AUTHORS   Yu,M., Yang,Y., Zhang,J., Liu,R., Huang,L., Wu,J., Kang,Z., Zhou,J.
            and Yang,Z.
  TITLE     Association between TRAF1/C5 Gene Polymorphisms and IgA Vasculitis
            in Chinese Children
  JOURNAL   Immunol Invest 53 (2), 281-293 (2024)
   PUBMED   38117213
  REMARK    GeneRIF: Association between TRAF1/C5 Gene Polymorphisms and IgA
            Vasculitis in Chinese Children.
REFERENCE   4  (bases 1 to 5482)
  AUTHORS   Liu,X., Hu,Y., Yu,X., Tan,Y., Yu,F., Chen,M. and Zhao,M.
  TITLE     Differential contributions of the C5b-9 and C5a/C5aR pathways to
            microvascular and macrovascular thrombosis in complement-mediated
            thrombotic microangiopathy patients
  JOURNAL   Clin Immunol 259, 109871 (2024)
   PUBMED   38101498
  REMARK    GeneRIF: Differential contributions of the C5b-9 and C5a/C5aR
            pathways to microvascular and macrovascular thrombosis in
            complement-mediated thrombotic microangiopathy patients.
REFERENCE   5  (bases 1 to 5482)
  AUTHORS   Merle,N.S., Church,S.E., Fremeaux-Bacchi,V. and Roumenina,L.T.
  TITLE     Complement System Part I - Molecular Mechanisms of Activation and
            Regulation
  JOURNAL   Front Immunol 6, 262 (2015)
   PUBMED   26082779
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5482)
  AUTHORS   DiScipio,R.G.
  TITLE     Formation and structure of the C5b-7 complex of the lytic pathway
            of complement
  JOURNAL   J Biol Chem 267 (24), 17087-17094 (1992)
   PUBMED   1387399
REFERENCE   7  (bases 1 to 5482)
  AUTHORS   Gerard,N.P. and Gerard,C.
  TITLE     The chemotactic receptor for human C5a anaphylatoxin
  JOURNAL   Nature 349 (6310), 614-617 (1991)
   PUBMED   1847994
REFERENCE   8  (bases 1 to 5482)
  AUTHORS   Haviland,D.L., Haviland,J.C., Fleischer,D.T., Hunt,A. and
            Wetsel,R.A.
  TITLE     Complete cDNA sequence of human complement pro-C5. Evidence of
            truncated transcripts derived from a single copy gene
  JOURNAL   J Immunol 146 (1), 362-368 (1991)
   PUBMED   1984448
REFERENCE   9  (bases 1 to 5482)
  AUTHORS   Tack,B.F., Morris,S.C. and Prahl,J.W.
  TITLE     Fifth component of human complement: purification from plasma and
            polypeptide chain structure
  JOURNAL   Biochemistry 18 (8), 1490-1497 (1979)
   PUBMED   106884
REFERENCE   10 (bases 1 to 5482)
  AUTHORS   Fernandez,H.N. and Hugli,T.E.
  TITLE     Primary structural analysis of the polypeptide portion of human C5a
            anaphylatoxin. Polypeptide sequence determination and assignment of
            the oligosaccharide attachment site in C5a
  JOURNAL   J Biol Chem 253 (19), 6955-6964 (1978)
   PUBMED   690134
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB209031.1, BC113738.1 and
            CB250401.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001317163.1.
            
            Summary: This gene encodes a component of the complement system, a
            part of the innate immune system that plays an important role in
            inflammation, host homeostasis, and host defense against pathogens.
            The encoded preproprotein is proteolytically processed to generate
            multiple protein products, including the C5 alpha chain, C5 beta
            chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of
            the C5 alpha and beta chains, which are linked by a disulfide
            bridge. Cleavage of the alpha chain by a convertase enzyme results
            in the formation of the C5a anaphylatoxin, which possesses potent
            spasmogenic and chemotactic activity, and the C5b macromolecular
            cleavage product, a subunit of the membrane attack complex (MAC).
            Mutations in this gene cause complement component 5 deficiency, a
            disease characterized by recurrent bacterial infections.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longest isoform (2). This isoform (2)
            lacks a predicted signal peptide compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB209031.1, SRR14038193.861960.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3114              AB209031.1         310-3423
            3115-3116           BC113738.1         3093-3094
            3117-5479           AB209031.1         3424-5786
            5480-5482           CB250401.1         16-18               c
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.2"
     gene            1..5482
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /note="complement C5"
                     /db_xref="GeneID:727"
                     /db_xref="HGNC:HGNC:1331"
                     /db_xref="MIM:120900"
     exon            1..112
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..5078
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /note="isoform 2 is encoded by transcript variant 2;
                     anaphylatoxin C5a analog; complement component 5; C3 and
                     PZP-like alpha-2-macroglobulin domain-containing protein
                     4; prepro-C5; C5a anaphylatoxin"
                     /codon_start=1
                     /product="complement C5 isoform 2"
                     /protein_id="NP_001304092.1"
                     /db_xref="CCDS:CCDS94473.1"
                     /db_xref="GeneID:727"
                     /db_xref="HGNC:HGNC:1331"
                     /db_xref="MIM:120900"
                     /translation="MPGSLGREASGRAGPTGCGAFAFGLRCRYVISAPKIFRVGASEN
                     IVIQVYGYTEAFDATISIKSYPDKKFSYSSGHVHLSSENKFQNSAILTIQPKQLPGGQ
                     NPVSYVYLEVVSKHFSKSKRMPITYDNGFLFIHTDKPVYTPDQSVKVRVYSLNDDLKP
                     AKRETVLTFIDPEGSEVDMVEEIDHIGIISFPDFKIPSNPRYGMWTIKAKYKEDFSTT
                     GTAYFEVKEYVLPHFSVSIEPEYNFIGYKNFKNFEITIKARYFYNKVVTEADVYITFG
                     IREDLKDDQKEMMQTAMQNTMLINGIAQVTFDSETAVKELSYYSLEDLNNKYLYIAVT
                     VIESTGGFSEEAEIPGIKYVLSPYKLNLVATPLFLKPGIPYPIKVQVKDSLDQLVGGV
                     PVTLNAQTIDVNQETSDLDPSKSVTRVDDGVASFVLNLPSGVTVLEFNVKTDAPDLPE
                     ENQAREGYRAIAYSSLSQSYLYIDWTDNHKALLVGEHLNIIVTPKSPYIDKITHYNYL
                     ILSKGKIIHFGTREKFSDASYQSINIPVTQNMVPSSRLLVYYIVTGEQTAELVSDSVW
                     LNIEEKCGNQLQVHLSPDADAYSPGQTVSLNMATGMDSWVALAAVDSAVYGVQRGAKK
                     PLERVFQFLEKSDLGCGAGGGLNNANVFHLAGLTFLTNANADDSQENDEPCKEILRPR
                     RTLQKKIEEIAAKYKHSVVKKCCYDGACVNNDETCEQRAARISLGPRCIKAFTECCVV
                     ASQLRANISHKDMQLGRLHMKTLLPVSKPEIRSYFPESWLWEVHLVPRRKQLQFALPD
                     SLTTWEIQGVGISNTGICVADTVKAKVFKDVFLEMNIPYSVVRGEQIQLKGTVYNYRT
                     SGMQFCVKMSAVEGICTSESPVIDHQGTKSSKCVRQKVEGSSSHLVTFTVLPLEIGLH
                     NINFSLETWFGKEILVKTLRVVPEGVKRESYSGVTLDPRGIYGTISRRKEFPYRIPLD
                     LVPKTEIKRILSVKGLLVGEILSAVLSQEGINILTHLPKGSAEAELMSVVPVFYVFHY
                     LETGNHWNIFHSDPLIEKQKLKKKLKEGMLSIMSYRNADYSYSVWKGGSASTWLTAFA
                     LRVLGQVNKYVEQNQNSICNSLLWLVENYQLDNGSFKENSQYQPIKLQGTLPVEAREN
                     SLYLTAFTVIGIRKAFDICPLVKIDTALIKADNFLLENTLPAQSTFTLAISAYALSLG
                     DKTHPQFRSIVSALKREALVKGNPPIYRFWKDNLQHKDSSVPNTGTARMVETTAYALL
                     TSLNLKDINYVNPVIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLRLSMDID
                     VSYKHKGALHNYKMTDKNFLGRPVEVLLNDDLIVSTGFGSGLATVHVTTVVHKTSTSE
                     EVCSFYLKIDTQDIEASHYRGYGNSDYKRIVACASYKPSREESSSGSSHAVMDISLPT
                     GISANEEDLKALVEGVDQLFTDYQIKDGHVILQLNSIPSSDFLCVRFRIFELFEVGFL
                     SPATFTVYEYHRPDKQCTMFYSTSNIKIQKVCEGAACKCVEADCGQMQEELDLTISAE
                     TRKQTACKPEIAYAYKVSITSITVENVFVKYKATLLDIYKTGEAVAEKDSEITFIKKV
                     TCTNAELVKGRQYLIMGKEALQIKYNFSFRYIYPLDSLTWIEYWPRDTTCSSCQAFLA
                     NLDEFAEDIFLNGC"
     exon            113..305
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            306..468
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            469..539
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            540..631
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            632..714
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            715..805
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            806..920
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1047
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1163
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1349
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1553
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1763
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1913
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..2043
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2106
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2304
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2395
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2469
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2470..2609
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2610..2837
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..2898
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2899..2988
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3201
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3202..3277
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3278..3437
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3438..3533
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3705
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3706..3911
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            3912..4064
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4065..4127
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4128..4209
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4210..4267
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4268..4370
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4371..4445
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4446..4551
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4552..4635
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4636..4725
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4726..4809
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4810..4948
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     exon            4949..5482
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5458..5463
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /note="hexamer: ATTAAA"
     polyA_site      5482
                     /gene="C5"
                     /gene_synonym="C5a; C5b; C5D; CPAMD4; ECLZB"
                     /note="major polyA site"
ORIGIN      
        1 cccgcctctt ttgcagccag cgcgctagga tgccggggag tcttgggcgc gaggcttccg
       61 gccgcgcggg gccgacggga tgcggtgcct tcgccttcgg attgcgttgc agatatgtca
      121 tttcagcacc aaaaatattc cgtgttggag catctgaaaa tattgtgatt caagtttatg
      181 gatacactga agcatttgat gcaacaatct ctattaaaag ttatcctgat aaaaaattta
      241 gttactcctc aggccatgtt catttatcct cagagaataa attccaaaac tctgcaatct
      301 taacaataca accaaaacaa ttgcctggag gacaaaaccc agtttcttat gtgtatttgg
      361 aagttgtatc aaagcatttt tcaaaatcaa aaagaatgcc aataacctat gacaatggat
      421 ttctcttcat tcatacagac aaacctgttt atactccaga ccagtcagta aaagttagag
      481 tttattcgtt gaatgacgac ttgaagccag ccaaaagaga aactgtctta actttcatag
      541 atcctgaagg atcagaagtt gacatggtag aagaaattga tcatattgga attatctctt
      601 ttcctgactt caagattccg tctaatccta gatatggtat gtggacgatc aaggctaaat
      661 ataaagagga cttttcaaca actggaaccg catattttga agttaaagaa tatgtcttgc
      721 cacatttttc tgtctcaatc gagccagaat ataatttcat tggttacaag aactttaaga
      781 attttgaaat tactataaaa gcaagatatt tttataataa agtagtcact gaggctgacg
      841 tttatatcac atttggaata agagaagact taaaagatga tcaaaaagaa atgatgcaaa
      901 cagcaatgca aaacacaatg ttgataaatg gaattgctca agtcacattt gattctgaaa
      961 cagcagtcaa agaactgtca tactacagtt tagaagattt aaacaacaag tacctttata
     1021 ttgctgtaac agtcatagag tctacaggtg gattttctga agaggcagaa atacctggca
     1081 tcaaatatgt cctctctccc tacaaactga atttggttgc tactcctctt ttcctgaagc
     1141 ctgggattcc atatcccatc aaggtgcagg ttaaagattc gcttgaccag ttggtaggag
     1201 gagtcccagt aacactgaat gcacaaacaa ttgatgtaaa ccaagagaca tctgacttgg
     1261 atccaagcaa aagtgtaaca cgtgttgatg atggagtagc ttcctttgtg cttaatctcc
     1321 catctggagt gacggtgctg gagtttaatg tcaaaactga tgctccagat cttccagaag
     1381 aaaatcaggc cagggaaggt taccgagcaa tagcatactc atctctcagc caaagttacc
     1441 tttatattga ttggactgat aaccataagg ctttgctagt gggagaacat ctgaatatta
     1501 ttgttacccc caaaagccca tatattgaca aaataactca ctataattac ttgattttat
     1561 ccaagggcaa aattatccac tttggcacga gggagaaatt ttcagatgca tcttatcaaa
     1621 gtataaacat tccagtaaca cagaacatgg ttccttcatc ccgacttctg gtctattaca
     1681 tcgtcacagg agaacagaca gcagaattag tgtctgattc agtctggtta aatattgaag
     1741 aaaaatgtgg caaccagctc caggttcatc tgtctcctga tgcagatgca tattctccag
     1801 gccaaactgt gtctcttaat atggcaactg gaatggattc ctgggtggca ttagcagcag
     1861 tggacagtgc tgtgtatgga gtccaaagag gagccaaaaa gcccttggaa agagtatttc
     1921 aattcttaga gaagagtgat ctgggctgtg gggcaggtgg tggcctcaac aatgccaatg
     1981 tgttccacct agctggactt accttcctca ctaatgcaaa tgcagatgac tcccaagaaa
     2041 atgatgaacc ttgtaaagaa attctcaggc caagaagaac gctgcaaaag aagatagaag
     2101 aaatagctgc taaatataaa cattcagtag tgaagaaatg ttgttacgat ggagcctgcg
     2161 ttaataatga tgaaacctgt gagcagcgag ctgcacggat tagtttaggg ccaagatgca
     2221 tcaaagcttt cactgaatgt tgtgtcgtcg caagccagct ccgtgctaat atctctcata
     2281 aagacatgca attgggaagg ctacacatga agaccctgtt accagtaagc aagccagaaa
     2341 ttcggagtta ttttccagaa agctggttgt gggaagttca tcttgttccc agaagaaaac
     2401 agttgcagtt tgccctacct gattctctaa ccacctggga aattcaaggc gttggcattt
     2461 caaacactgg tatatgtgtt gctgatactg tcaaggcaaa ggtgttcaaa gatgtcttcc
     2521 tggaaatgaa tataccatat tctgttgtac gaggagaaca gatccaattg aaaggaactg
     2581 tttacaacta taggacttct gggatgcagt tctgtgttaa aatgtctgct gtggagggaa
     2641 tctgcacttc ggaaagccca gtcattgatc atcagggcac aaagtcctcc aaatgtgtgc
     2701 gccagaaagt agagggctcc tccagtcact tggtgacatt cactgtgctt cctctggaaa
     2761 ttggccttca caacatcaat ttttcactgg agacttggtt tggaaaagaa atcttagtaa
     2821 aaacattacg agtggtgcca gaaggtgtca aaagggaaag ctattctggt gttactttgg
     2881 atcctagggg tatttatggt accattagca gacgaaagga gttcccatac aggataccct
     2941 tagatttggt ccccaaaaca gaaatcaaaa ggattttgag tgtaaaagga ctgcttgtag
     3001 gtgagatctt gtctgcagtt ctaagtcagg aaggcatcaa tatcctaacc cacctcccca
     3061 aagggagtgc agaggcggag ctgatgagcg ttgtcccagt attctatgtt tttcactacc
     3121 tggaaacagg aaatcattgg aacatttttc attctgaccc attaattgaa aagcagaaac
     3181 tgaagaaaaa attaaaagaa gggatgttga gcattatgtc ctacagaaat gctgactact
     3241 cttacagtgt gtggaagggt ggaagtgcta gcacttggtt aacagctttt gctttaagag
     3301 tacttggaca agtaaataaa tacgtagagc agaaccaaaa ttcaatttgt aattctttat
     3361 tgtggctagt tgagaattat caattagata atggatcttt caaggaaaat tcacagtatc
     3421 aaccaataaa attacagggt accttgcctg ttgaagcccg agagaacagc ttatatctta
     3481 cagcctttac tgtgattgga attagaaagg ctttcgatat atgccccctg gtgaaaatcg
     3541 acacagctct aattaaagct gacaactttc tgcttgaaaa tacactgcca gcccagagca
     3601 cctttacatt ggccatttct gcgtatgctc tttccctggg agataaaact cacccacagt
     3661 ttcgttcaat tgtttcagct ttgaagagag aagctttggt taaaggtaat ccacccattt
     3721 atcgtttttg gaaagacaat cttcagcata aagacagctc tgtacctaac actggtacgg
     3781 cacgtatggt agaaacaact gcctatgctt tactcaccag tctgaacttg aaagatataa
     3841 attatgttaa cccagtcatc aaatggctat cagaagagca gaggtatgga ggtggctttt
     3901 attcaaccca ggacacaatc aatgccattg agggcctgac ggaatattca ctcctggtta
     3961 aacaactccg cttgagtatg gacatcgatg tttcttacaa gcataaaggt gccttacata
     4021 attataaaat gacagacaag aatttccttg ggaggccagt agaggtgctt ctcaatgatg
     4081 acctcattgt cagtacagga tttggcagtg gcttggctac agtacatgta acaactgtag
     4141 ttcacaaaac cagtacctct gaggaagttt gcagctttta tttgaaaatc gatactcagg
     4201 atattgaagc atcccactac agaggctacg gaaactctga ttacaaacgc atagtagcat
     4261 gtgccagcta caagcccagc agggaagaat catcatctgg atcctctcat gcggtgatgg
     4321 acatctcctt gcctactgga atcagtgcaa atgaagaaga cttaaaagcc cttgtggaag
     4381 gggtggatca actattcact gattaccaaa tcaaagatgg acatgttatt ctgcaactga
     4441 attcgattcc ctccagtgat ttcctttgtg tacgattccg gatatttgaa ctctttgaag
     4501 ttgggtttct cagtcctgcc actttcacag tgtacgaata ccacagacca gataaacagt
     4561 gtaccatgtt ttatagcact tccaatatca aaattcagaa agtctgtgaa ggagccgcgt
     4621 gcaagtgtgt agaagctgat tgtgggcaaa tgcaggaaga attggatctg acaatctctg
     4681 cagagacaag aaaacaaaca gcatgtaaac cagagattgc atatgcttat aaagttagca
     4741 tcacatccat cactgtagaa aatgtttttg tcaagtacaa ggcaaccctt ctggatatct
     4801 acaaaactgg ggaagctgtt gctgagaaag actctgagat taccttcatt aaaaaggtaa
     4861 cctgtactaa cgctgagctg gtaaaaggaa gacagtactt aattatgggt aaagaagccc
     4921 tccagataaa atacaatttc agtttcaggt acatctaccc tttagattcc ttgacctgga
     4981 ttgaatactg gcctagagac acaacatgtt catcgtgtca agcattttta gctaatttag
     5041 atgaatttgc cgaagatatc tttttaaatg gatgctaaaa ttcctgaagt tcagctgcat
     5101 acagtttgca cttatggact cctgttgttg aagttcgttt ttttgttttc ttcttttttt
     5161 aaacattcat agctggtctt atttgtaaag ctcactttac ttagaattag tggcacttgc
     5221 ttttattaga gaatgatttc aaatgctgta actttctgaa ataacatggc cttggagggc
     5281 atgaagacag atactcctcc aaggttattg gacaccggaa acaataaatt ggaacacctc
     5341 ctcaaaccta ccactcagga atgtttgctg gggccgaaag aacagtccat tgaaagggag
     5401 tattacaaaa acatggcctt tgcttgaaag aaaataccaa ggaacaggaa actgatcatt
     5461 aaagcctgag tttgctttca aa
//
