LOCUS       NM_007293               5427 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens complement C4A (Chido/Rodgers blood group) (C4A),
            transcript variant 1, mRNA.
ACCESSION   NM_007293 XM_003403789 XM_003960046 XM_006725947 XM_011548125
VERSION     NM_007293.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Mariaselvam,C.M., Seth,G., Kavadichanda,C., Boukouaci,W., Wu,C.L.,
            Costes,B., Thabah,M.M., Krishnamoorthy,R., Leboyer,M., Negi,V.S.
            and Tamouza,R.
  TITLE     Low C4A copy numbers and higher HERV gene insertion contributes to
            increased risk of SLE, with absence of association with disease
            phenotype and disease activity
  JOURNAL   Immunol Res 72 (4), 697-706 (2024)
   PUBMED   38594415
  REMARK    GeneRIF: Low C4A copy numbers and higher HERV gene insertion
            contributes to increased risk of SLE, with absence of association
            with disease phenotype and disease activity.
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Hernandez,L.M., Kim,M., Zhang,P., Bethlehem,R.A.I., Hoftman,G.,
            Loughnan,R., Smith,D., Bookheimer,S.Y., Fan,C.C., Bearden,C.E.,
            Thompson,W.K. and Gandal,M.J.
  TITLE     Multi-ancestry phenome-wide association of complement component 4
            variation with psychiatric and brain phenotypes in youth
  JOURNAL   Genome Biol 24 (1), 42 (2023)
   PUBMED   36882872
  REMARK    GeneRIF: Multi-ancestry phenome-wide association of complement
            component 4 variation with psychiatric and brain phenotypes in
            youth.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5427)
  AUTHORS   Gracias,J., Orhan,F., Horbeck,E., Holmen-Larsson,J.,
            Khanlarkani,N., Malwade,S., Goparaju,S.K., Schwieler,L.,
            Demirel,I.S., Fu,T., Fatourus-Bergman,H., Pelanis,A., Goold,C.P.,
            Goulding,A., Annerbrink,K., Isgren,A., Sparding,T., Schalling,M.,
            Yanez,V.A.C., Gopfert,J.C., Nilsson,J., Brinkmalm,A., Blennow,K.,
            Zetterberg,H., Engberg,G., Piehl,F., Sheridan,S.D., Perlis,R.H.,
            Cervenka,S., Erhardt,S., Landen,M. and Sellgren,C.M.
  TITLE     Cerebrospinal fluid concentration of complement component 4A is
            increased in first episode schizophrenia
  JOURNAL   Nat Commun 13 (1), 6427 (2022)
   PUBMED   36329007
  REMARK    GeneRIF: Cerebrospinal fluid concentration of complement component
            4A is increased in first episode schizophrenia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5427)
  AUTHORS   Pasupuleti,M., Walse,B., Nordahl,E.A., Morgelin,M., Malmsten,M. and
            Schmidtchen,A.
  TITLE     Preservation of antimicrobial properties of complement peptide C3a,
            from invertebrates to humans
  JOURNAL   J Biol Chem 282 (4), 2520-2528 (2007)
   PUBMED   17132627
  REMARK    GeneRIF: Complement components C3a and C4a, but not C5a, display
            antimicrobial activity against P. aeruginosa, E. coli, B. subtilis,
            and C. albicans.
REFERENCE   5  (bases 1 to 5427)
  AUTHORS   Anderson,M.J., Milner,C.M., Cotton,R.G. and Campbell,R.D.
  TITLE     The coding sequence of the hemolytically inactive C4A6 allotype of
            human complement component C4 reveals that a single arginine to
            tryptophan substitution at beta-chain residue 458 is the likely
            cause of the defect
  JOURNAL   J Immunol 148 (9), 2795-2802 (1992)
   PUBMED   1573268
REFERENCE   6  (bases 1 to 5427)
  AUTHORS   Yu,C.Y.
  TITLE     The complete exon-intron structure of a human complement component
            C4A gene. DNA sequences, polymorphism, and linkage to the
            21-hydroxylase gene
  JOURNAL   J Immunol 146 (3), 1057-1066 (1991)
   PUBMED   1988494
REFERENCE   7  (bases 1 to 5427)
  AUTHORS   Palsdottir,A., Fossdal,R., Arnason,A., Edwards,J.H. and Jensson,O.
  TITLE     Heterogeneity of human C4 gene size. A large intron (6.5 kb) is
            present in all C4A genes and some C4B genes
  JOURNAL   Immunogenetics 25 (5), 299-304 (1987)
   PUBMED   2883116
REFERENCE   8  (bases 1 to 5427)
  AUTHORS   Yu,C.Y., Belt,K.T., Giles,C.M., Campbell,R.D. and Porter,R.R.
  TITLE     Structural basis of the polymorphism of human complement components
            C4A and C4B: gene size, reactivity and antigenicity
  JOURNAL   EMBO J 5 (11), 2873-2881 (1986)
   PUBMED   2431902
REFERENCE   9  (bases 1 to 5427)
  AUTHORS   Belt,K.T., Carroll,M.C. and Porter,R.R.
  TITLE     The structural basis of the multiple forms of human complement
            component C4
  JOURNAL   Cell 36 (4), 907-914 (1984)
   PUBMED   6546707
REFERENCE   10 (bases 1 to 5427)
  AUTHORS   Fearon,D.T.
  TITLE     Activation of the alternative complement pathway
  JOURNAL   CRC Crit Rev Immunol 1 (1), 1-32 (1979)
   PUBMED   162484
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA304049.1, K02403.1,
            AL645922.12, BC146673.1 and BC016933.1.
            
            On Aug 1, 2019 this sequence version replaced NM_007293.2.
            
            Summary: This gene encodes the acidic form of complement factor 4,
            part of the classical activation pathway. The protein is expressed
            as a single chain precursor which is proteolytically cleaved into a
            trimer of alpha, beta, and gamma chains prior to secretion. The
            trimer provides a surface for interaction between the
            antigen-antibody complex and other complement components. The alpha
            chain is cleaved to release C4 anaphylatoxin, an antimicrobial
            peptide and a mediator of local inflammation. Deficiency of this
            protein is associated with systemic lupus erythematosus and type I
            diabetes mellitus. This gene localizes to the major
            histocompatibility complex (MHC) class III region on chromosome 6.
            Varying haplotypes of this gene cluster exist, such that
            individuals may have 1, 2, or 3 copies of this gene. Two transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record represents the C4A*001.1.1
            allele.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.237372.1,
                                           SRR1803615.258018.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match                 :: ENST00000428956.7/
                                                  ENSP00000396688.2
            Protein has antimicrobial activity :: PMID: 17132627
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression,
                                                  longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-555               DA304049.1         1-555
            556-1303            K02403.1           545-1292
            1304-1304           AL645922.12        87764-87764
            1305-3651           K02403.1           1294-3640
            3652-4422           BC146673.1         3641-4411
            4423-5426           K02403.1           4403-5406
            5427-5427           BC016933.1         803-803
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.33"
     gene            1..5427
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /note="complement C4A (Chido/Rodgers blood group)"
                     /db_xref="GeneID:720"
                     /db_xref="HGNC:HGNC:1323"
                     /db_xref="MIM:120810"
     exon            1..116
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..5286
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; acidic C4; Rodgers form of C4; C4A
                     anaphylatoxin; complement C4-A; acidic complement C4; C3
                     and PZP-like alpha-2-macroglobulin domain-containing
                     protein 2; MHC class III region complement; complement
                     component 4A (Rodgers blood group); complement C4A
                     (Rodgers blood group)"
                     /codon_start=1
                     /product="complement C4-A isoform 1 preproprotein"
                     /protein_id="NP_009224.2"
                     /db_xref="CCDS:CCDS47404.1"
                     /db_xref="GeneID:720"
                     /db_xref="HGNC:HGNC:1323"
                     /db_xref="MIM:120810"
                     /translation="MRLLWGLIWASSFFTLSLQKPRLLLFSPSVVHLGVPLSVGVQLQ
                     DVPRGQVVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQL
                     LRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRV
                     FALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISAR
                     FSDGLESNSSTQFEVKKYVLPNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPV
                     QGVAYVRFGLLDEDGKKTFFRGLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQ
                     GLRLYVAAAIIESPGGEMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVRE
                     MSGSPASGIPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSA
                     GSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGATFSHYYYM
                     ILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHGDHPVANSLRVDVQAGA
                     CEGKLELSVDGAKQYRNGESVKLHLETDSLALVALGALDTALYAAGSKSHKPLNMGKV
                     FEAMNSYDLGCGPGGGDSALQVFQAAGLAFSDGDQWTLSRKRLSCPKEKTTRKKRNVN
                     FQKAINEKLGQYASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAE
                     SLRKKSRDKGQAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLW
                     LPDSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLY
                     NYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVV
                     ARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDG
                     DFNSYVRVTASDPLDTLGSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDK
                     TEQWSTLPPETKDHAVDLIQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSL
                     AQEQVGGSPEKLQETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVT
                     IALHHGLAVFQDEGAEPLKQRVEASISKANSFLGEKASAGLLGAHAAAITAYALTLTK
                     APVDLLGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNPSDPMPQAPALWI
                     ETTAYALLHLLLHEGKAEMADQASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASH
                     TTEERGLNVTLSSTGRNGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTL
                     KVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQ
                     PVTPLQLFEGRRNRRRREAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGF
                     HALRADLEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQPA
                     SATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDED
                     GYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQVLHFTKDVKAAANQMRNFL
                     VRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLDSNSWIEEMPSERLCRSTRQRAA
                     CAQLNDFLQEYGTQGCQV"
     exon            117..315
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            316..517
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            518..588
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            589..677
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            678..760
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            761..857
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            858..963
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1096
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1212
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1392
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1575
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1761
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1920
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2047
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2122
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2320
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..2432
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2503
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2504..2643
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2853
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..2905
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..2995
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3205
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            3206..3281
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            3282..3438
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            3439..3555
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            3556..3727
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            3728..3960
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            3961..4128
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4129..4188
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4189..4282
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4283..4469
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4470..4560
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4561..4635
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4636..4738
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4739..4828
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4829..4927
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            4928..5011
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            5012..5144
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
     exon            5145..5427
                     /gene="C4A"
                     /gene_synonym="C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4;
                     CPAMD2; RG"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agaaggtagc agacagacag acggatctaa cctctcttgg atcctccagc catgaggctg
       61 ctctgggggc tgatctgggc atccagcttc ttcaccttat ctctgcagaa gcccaggttg
      121 ctcttgttct ctccttctgt ggttcatctg ggggtccccc tatcggtggg ggtgcagctc
      181 caggatgtgc cccgaggaca ggtagtgaaa ggatcagtgt tcctgagaaa cccatctcgt
      241 aataatgtcc cctgctcccc aaaggtggac ttcaccctta gctcagaaag agacttcgca
      301 ctcctcagtc tccaggtgcc cttgaaagat gcgaagagct gtggcctcca tcaactcctc
      361 agaggccctg aggtccagct ggtggcccat tcgccatggc taaaggactc tctgtccaga
      421 acgacaaaca tccagggtat caacctgctc ttctcctctc gccgggggca cctctttttg
      481 cagacggacc agcccattta caaccctggc cagcgggttc ggtaccgggt ctttgctctg
      541 gatcagaaga tgcgcccgag cactgacacc atcacagtca tggtggagaa ctctcacggc
      601 ctccgcgtgc ggaagaagga ggtgtacatg ccctcgtcca tcttccagga tgactttgtg
      661 atcccagaca tctcagagcc agggacctgg aagatctcag cccgattctc agatggcctg
      721 gaatccaaca gcagcaccca gtttgaggtg aagaaatatg tccttcccaa ctttgaggtg
      781 aagatcaccc ctggaaagcc ctacatcctg acggtgccag gccatcttga tgaaatgcag
      841 ttagacatcc aggccaggta catctatggg aagccagtgc agggggtggc atatgtgcgc
      901 tttgggctcc tagatgagga tggtaagaag actttctttc gggggctgga gagtcagacc
      961 aagctggtga atggacagag ccacatttcc ctctcaaagg cagagttcca ggacgccctg
     1021 gagaagctga atatgggcat tactgacctc caggggctgc gcctctacgt tgctgcagcc
     1081 atcattgagt ctccaggtgg ggagatggag gaggcagagc tcacatcctg gtattttgtg
     1141 tcatctccct tctccttgga tcttagcaag accaagcgac accttgtgcc tggggccccc
     1201 ttcctgctgc aggccttggt ccgtgagatg tcaggctccc cagcttctgg cattcctgtc
     1261 aaagtttctg ccacggtgtc ttctcctggg tctgttcctg aagtccagga cattcagcaa
     1321 aacacagacg ggagcggcca agtcagcatt ccaataatta tccctcagac catctcagag
     1381 ctgcagctct cagtatctgc aggctcccca catccagcga tagccaggct cactgtggca
     1441 gccccacctt caggaggccc cgggtttctg tctattgagc ggccggattc tcgacctcct
     1501 cgtgttgggg acactctgaa cctgaacttg cgagccgtgg gcagtggggc caccttttct
     1561 cattactact acatgatcct atcccgaggg cagatcgtgt tcatgaatcg agagcccaag
     1621 aggaccctga cctcggtctc ggtgtttgtg gaccatcacc tggcaccctc cttctacttt
     1681 gtggccttct actaccatgg agaccaccca gtggccaact ccctgcgagt ggatgtccag
     1741 gctggggcct gcgagggcaa gctggagctc agcgtggacg gtgccaagca gtaccggaac
     1801 ggggagtccg tgaagctcca cttagaaacc gactccctag ccctggtggc gctgggagcc
     1861 ttggacacag ctctgtatgc tgcaggcagc aagtcccaca agcccctcaa catgggcaag
     1921 gtctttgaag ctatgaacag ctatgacctc ggctgtggtc ctgggggtgg ggacagtgcc
     1981 cttcaggtgt tccaggcagc gggcctggcc ttttctgatg gagaccagtg gaccttatcc
     2041 agaaagagac taagctgtcc caaggagaag acaacccgga aaaagagaaa cgtgaacttc
     2101 caaaaggcga ttaatgagaa attgggtcag tatgcttccc cgacagccaa gcgctgctgc
     2161 caggatgggg tgacacgtct gcccatgatg cgttcctgcg agcagcgggc agcccgcgtg
     2221 cagcagccgg actgccggga gcccttcctg tcctgctgcc aatttgctga gagtctgcgc
     2281 aagaagagca gggacaaggg ccaggcgggc ctccaacgag ccctggagat cctgcaggag
     2341 gaggacctga ttgatgagga tgacattccc gtgcgcagct tcttcccaga gaactggctc
     2401 tggagagtgg aaacagtgga ccgctttcaa atattgacac tgtggctccc cgactctctg
     2461 accacgtggg agatccatgg cctgagcctg tccaaaacca aaggcctatg tgtggccacc
     2521 ccagtccagc tccgggtgtt ccgcgagttc cacctgcacc tccgcctgcc catgtctgtc
     2581 cgccgctttg agcagctgga gctgcggcct gtcctctata actacctgga taaaaacctg
     2641 actgtgagcg tccacgtgtc cccagtggag gggctgtgcc tggctggggg cggagggctg
     2701 gcccagcagg tgctggtgcc tgcgggctct gcccggcctg ttgccttctc tgtggtgccc
     2761 acggcagccg ccgctgtgtc tctgaaggtg gtggctcgag ggtccttcga attccctgtg
     2821 ggagatgcgg tgtccaaggt tctgcagatt gagaaggaag gggccatcca tagagaggag
     2881 ctggtctatg aactcaaccc cttggaccac cgaggccgga ccttggaaat acctggcaac
     2941 tctgatccca atatgatccc tgatggggac tttaacagct acgtcagggt tacagcctca
     3001 gatccattgg acactttagg ctctgagggg gccttgtcac caggaggcgt ggcctccctc
     3061 ttgaggcttc ctcgaggctg tggggagcaa accatgatct acttggctcc gacactggct
     3121 gcttcccgct acctggacaa gacagagcag tggagcacac tgcctcccga gaccaaggac
     3181 cacgccgtgg atctgatcca gaaaggctac atgcggatcc agcagtttcg gaaggcggat
     3241 ggttcctatg cggcttggtt gtcacgggac agcagcacct ggctcacagc ctttgtgttg
     3301 aaggtcctga gtttggccca ggagcaggta ggaggctcgc ctgagaaact gcaggagaca
     3361 tctaactggc ttctgtccca gcagcaggct gacggctcgt tccaggaccc ctgtccagtg
     3421 ttagacagga gcatgcaggg gggtttggtg ggcaatgatg agactgtggc actcacagcc
     3481 tttgtgacca tcgcccttca tcatgggctg gccgtcttcc aggatgaggg tgcagagcca
     3541 ttgaagcaga gagtggaagc ctccatctca aaggcaaact catttttggg ggagaaagca
     3601 agtgctgggc tcctgggtgc ccacgcagct gccatcacgg cctatgccct gacactgacc
     3661 aaggcgcctg tggacctgct cggtgttgcc cacaacaacc tcatggcaat ggcccaggag
     3721 actggagata acctgtactg gggctcagtc actggttctc agagcaatgc cgtgtcgccc
     3781 accccggctc ctcgcaaccc atccgacccc atgccccagg ccccagccct gtggattgaa
     3841 accacagcct acgccctgct gcacctcctg cttcacgagg gcaaagcaga gatggcagac
     3901 caggcttcgg cctggctcac ccgtcagggc agcttccaag ggggattccg cagtacccaa
     3961 gacacggtga ttgccctgga tgccctgtct gcctactgga ttgcctccca caccactgag
     4021 gagaggggtc tcaatgtgac tctcagctcc acaggccgga atgggttcaa gtcccacgcg
     4081 ctgcagctga acaaccgcca gattcgcggc ctggaggagg agctgcagtt ttccttgggc
     4141 agcaagatca atgtgaaggt gggaggaaac agcaaaggaa ccctgaaggt ccttcgtacc
     4201 tacaatgtcc tggacatgaa gaacacgacc tgccaggacc tacagataga agtgacagtc
     4261 aaaggccacg tcgagtacac gatggaagca aacgaggact atgaggacta tgagtacgat
     4321 gagcttccag ccaaggatga cccagatgcc cctctgcagc ccgtgacacc cctgcagctg
     4381 tttgagggtc ggaggaaccg ccgcaggagg gaggcgccca aggtggtgga ggagcaggag
     4441 tccagggtgc actacaccgt gtgcatctgg cggaacggca aggtggggct gtctggcatg
     4501 gccatcgcgg acgtcaccct cctgagtgga ttccacgccc tgcgtgctga cctggagaag
     4561 ctgacctccc tctctgaccg ttacgtgagt cactttgaga ccgaggggcc ccacgtcctg
     4621 ctgtattttg actcggtccc cacctcccgg gagtgcgtgg gctttgaggc tgtgcaggaa
     4681 gtgccggtgg ggctggtgca gccggccagc gcaaccctgt acgactacta caaccccgag
     4741 cgcagatgtt ctgtgtttta cggggcacca agtaagagca gactcttggc caccttgtgt
     4801 tctgctgaag tctgccagtg tgctgagggg aagtgccctc gccagcgtcg cgccctggag
     4861 cggggtctgc aggacgagga tggctacagg atgaagtttg cctgctacta cccccgtgtg
     4921 gagtacggct tccaggttaa ggttctccga gaagacagca gagctgcttt ccgcctcttt
     4981 gagaccaaga tcacccaagt cctgcacttc accaaggatg tcaaggccgc tgctaatcag
     5041 atgcgcaact tcctggttcg agcctcctgc cgccttcgct tggaacctgg gaaagaatat
     5101 ttgatcatgg gtctggatgg ggccacctat gacctcgagg gacaccccca gtacctgctg
     5161 gactcgaata gctggatcga ggagatgccc tctgaacgcc tgtgccggag cacccgccag
     5221 cgggcagcct gtgcccagct caacgacttc ctccaggagt atggcactca ggggtgccag
     5281 gtgtgagggc tgccctccca cctccgctgg gaggaacctg aacctgggaa ccatgaagct
     5341 ggaagcactg ctgtgtccgc tttcatgaac acagcctggg accagggcat attaaaggct
     5401 tttggcagca aagtgtcagt gttggca
//
