LOCUS       NM_000064               5231 bp    mRNA    linear   PRI 10-OCT-2024
DEFINITION  Homo sapiens complement C3 (C3), mRNA.
ACCESSION   NM_000064
VERSION     NM_000064.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ge,T.Q., Guan,P.P. and Wang,P.
  TITLE     Complement 3a induces the synapse loss via C3aR in
            mitochondria-dependent NLRP3 activating mechanisms during the
            development and progression of Alzheimer's disease
  JOURNAL   Neurosci Biobehav Rev 165, 105868 (2024)
   PUBMED   39218048
  REMARK    GeneRIF: Complement 3a induces the synapse loss via C3aR in
            mitochondria-dependent NLRP3 activating mechanisms during the
            development and progression of Alzheimer's disease.
            Review article
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Chou,Y.L., Hsu,Y.A., Lin,C.F., Chen,C.S., Tien,P.T., Wang,Y.C.,
            Chang,C.Y., Lin,E.S., Chen,J.J., Wu,M.Y., Chuang,C.Y., Lin,H.J. and
            Wan,L.
  TITLE     Complement decay-accelerating factor inhibits inflammation-induced
            myopia development
  JOURNAL   Mol Immunol 171, 47-55 (2024)
   PUBMED   38795684
  REMARK    GeneRIF: Complement decay-accelerating factor inhibits
            inflammation-induced myopia development.
REFERENCE   3  (bases 1 to 5231)
  AUTHORS   Shim,K.H., Kim,D., Kang,M.J., Pyun,J.M., Park,Y.H., Youn,Y.C.,
            Park,K.W., Suk,K., Lee,H.W., Gomes,B.F., Zetterberg,H., An,S.S.A.
            and Kim,S.
  CONSRTM   Alzheimer's Disease All Markers (ADAM) Research group
  TITLE     Subsequent correlated changes in complement component 3 and amyloid
            beta oligomers in the blood of patients with Alzheimer's disease
  JOURNAL   Alzheimers Dement 20 (4), 2731-2741 (2024)
   PUBMED   38411315
  REMARK    GeneRIF: Subsequent correlated changes in complement component 3
            and amyloid beta oligomers in the blood of patients with
            Alzheimer's disease.
REFERENCE   4  (bases 1 to 5231)
  AUTHORS   Zimmer,J., Hobkirk,J., Mohamed,F., Browning,M.J. and Stover,C.M.
  TITLE     On the Functional Overlap between Complement and Anti-Microbial
            Peptides
  JOURNAL   Front Immunol 5, 689 (2015)
   PUBMED   25646095
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5231)
  AUTHORS   Nordahl,E.A., Rydengard,V., Nyberg,P., Nitsche,D.P., Morgelin,M.,
            Malmsten,M., Bjorck,L. and Schmidtchen,A.
  TITLE     Activation of the complement system generates antibacterial
            peptides
  JOURNAL   Proc Natl Acad Sci U S A 101 (48), 16879-16884 (2004)
   PUBMED   15550543
  REMARK    GeneRIF: A peptide (C3a) derived from the complement C3 protein has
            antimicrobial activity against E. coli, P. aeruginosa, E. faecalis,
            and C. albicans.
REFERENCE   6  (bases 1 to 5231)
  AUTHORS   Martin,B. and Smith,R.J.H.
  TITLE     C3 Glomerulopathy
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301598
REFERENCE   7  (bases 1 to 5231)
  AUTHORS   Noris,M., Bresin,E., Mele,C. and Remuzzi,G.
  TITLE     Genetic Atypical Hemolytic-Uremic Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301541
REFERENCE   8  (bases 1 to 5231)
  AUTHORS   de Bruijn,M.H. and Fey,G.H.
  TITLE     Human complement component C3: cDNA coding sequence and derived
            primary structure
  JOURNAL   Proc Natl Acad Sci U S A 82 (3), 708-712 (1985)
   PUBMED   2579379
REFERENCE   9  (bases 1 to 5231)
  AUTHORS   Fearon,D.T.
  TITLE     Activation of the alternative complement pathway
  JOURNAL   CRC Crit Rev Immunol 1 (1), 1-32 (1979)
   PUBMED   162484
  REMARK    Review article
REFERENCE   10 (bases 1 to 5231)
  AUTHORS   Schreiber,R.D., Pangburn,M.K., Lesavre,P.H. and
            Muller-Eberhard,H.J.
  TITLE     Initiation of the alternative pathway of complement: recognition of
            activators by bound C3b and assembly of the entire pathway from six
            isolated proteins
  JOURNAL   Proc Natl Acad Sci U S A 75 (8), 3948-3952 (1978)
   PUBMED   279011
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK299114.1, K02765.1,
            BC150179.1, BC150299.1, AC008760.7 and AA025232.1.
            
            On May 17, 2019 this sequence version replaced NM_000064.3.
            
            Summary: Complement component C3 plays a central role in the
            activation of complement system. Its activation is required for
            both classical and alternative complement activation pathways. The
            encoded preproprotein is proteolytically processed to generate
            alpha and beta subunits that form the mature protein, which is then
            further processed to generate numerous peptide products. The C3a
            peptide, also known as the C3a anaphylatoxin, modulates
            inflammation and possesses antimicrobial activity. Mutations in
            this gene are associated with atypical hemolytic uremic syndrome
            and age-related macular degeneration in human patients. [provided
            by RefSeq, Nov 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150299.1, SRR1660807.97673.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN04284274 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match                 :: ENST00000245907.11/
                                                  ENSP00000245907.4
            Protein has antimicrobial activity :: PMID: 15550543
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AK299114.1         33-33
            2-1001              K02765.1           1-1000
            1002-1896           BC150179.1         973-1867
            1897-2481           K02765.1           1896-2480
            2482-2715           BC150299.1         2482-2715
            2716-2716           AC008760.7         133171-133171       c
            2717-3042           BC150299.1         2716-3041
            3043-5095           BC150179.1         3014-5066
            5096-5100           AA025232.1         17-21               c
            5101-5231           AC008760.7         113390-113520       c
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..5231
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /note="complement C3"
                     /db_xref="GeneID:718"
                     /db_xref="HGNC:HGNC:1318"
                     /db_xref="MIM:120700"
     exon            1..135
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..5053
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /EC_number="3.4.21.43"
                     /note="acylation-stimulating protein cleavage product;
                     prepro-C3; C3a anaphylatoxin; complement component C3a;
                     complement component C3b; epididymis secretory sperm
                     binding protein Li 62p; C3 and PZP-like
                     alpha-2-macroglobulin domain-containing protein 1;
                     complement component 3"
                     /codon_start=1
                     /product="complement C3 preproprotein"
                     /protein_id="NP_000055.2"
                     /db_xref="CCDS:CCDS32883.1"
                     /db_xref="GeneID:718"
                     /db_xref="HGNC:HGNC:1318"
                     /db_xref="MIM:120700"
                     /translation="MGPTSGPSLLLLLLTHLPLALGSPMYSIITPNILRLESEETMVL
                     EAHDAQGDVPVTVTVHDFPGKKLVLSSEKTVLTPATNHMGNVTFTIPANREFKSEKGR
                     NKFVTVQATFGTQVVEKVVLVSLQSGYLFIQTDKTIYTPGSTVLYRIFTVNHKLLPVG
                     RTVMVNIENPEGIPVKQDSLSSQNQLGVLPLSWDIPELVNMGQWKIRAYYENSPQQVF
                     STEFEVKEYVLPSFEVIVEPTEKFYYIYNEKGLEVTITARFLYGKKVEGTAFVIFGIQ
                     DGEQRISLPESLKRIPIEDGSGEVVLSRKVLLDGVQNPRAEDLVGKSLYVSATVILHS
                     GSDMVQAERSGIPIVTSPYQIHFTKTPKYFKPGMPFDLMVFVTNPDGSPAYRVPVAVQ
                     GEDTVQSLTQGDGVAKLSINTHPSQKPLSITVRTKKQELSEAEQATRTMQALPYSTVG
                     NSNNYLHLSVLRTELRPGETLNVNFLLRMDRAHEAKIRYYTYLIMNKGRLLKAGRQVR
                     EPGQDLVVLPLSITTDFIPSFRLVAYYTLIGASGQREVVADSVWVDVKDSCVGSLVVK
                     SGQSEDRQPVPGQQMTLKIEGDHGARVVLVAVDKGVFVLNKKNKLTQSKIWDVVEKAD
                     IGCTPGSGKDYAGVFSDAGLTFTSSSGQQTAQRAELQCPQPAARRRRSVQLTEKRMDK
                     VGKYPKELRKCCEDGMRENPMRFSCQRRTRFISLGEACKKVFLDCCNYITELRRQHAR
                     ASHLGLARSNLDEDIIAEENIVSRSEFPESWLWNVEDLKEPPKNGISTKLMNIFLKDS
                     ITTWEILAVSMSDKKGICVADPFEVTVMQDFFIDLRLPYSVVRNEQVEIRAVLYNYRQ
                     NQELKVRVELLHNPAFCSLATTKRRHQQTVTIPPKSSLSVPYVIVPLKTGLQEVEVKA
                     AVYHHFISDGVRKSLKVVPEGIRMNKTVAVRTLDPERLGREGVQKEDIPPADLSDQVP
                     DTESETRILLQGTPVAQMTEDAVDAERLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDE
                     TEQWEKFGLEKRQGALELIKKGYTQQLAFRQPSSAFAAFVKRAPSTWLTAYVVKVFSL
                     AVNLIAIDSQVLCGAVKWLILEKQKPDGVFQEDAPVIHQEMIGGLRNNNEKDMALTAF
                     VLISLQEAKDICEEQVNSLPGSITKAGDFLEANYMNLQRSYTVAIAGYALAQMGRLKG
                     PLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYY
                     GGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKITHRIHWESASLLR
                     SEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRPQ
                     DAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDK
                     AFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYH
                     PEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKV
                     QLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSD
                     FWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN"
     exon            136..328
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            329..494
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            495..565
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            566..660
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            661..743
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            744..834
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            835..937
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1064
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1180
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1330
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1540
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1747
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1906
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2036
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2108
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..2306
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2415
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2501
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2644
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            2645..2857
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            2858..2924
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            2925..3011
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            3012..3215
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            3216..3291
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            3292..3451
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            3452..3550
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            3551..3707
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            3708..3871
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            3872..4030
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4031..4090
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4091..4181
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4182..4233
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4234..4321
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4322..4411
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4412..4517
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4518..4607
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4608..4691
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4692..4775
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4776..4911
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     exon            4912..5231
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5078..5083
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /note="hexamer: GATAAA"
     polyA_site      5100
                     /gene="C3"
                     /gene_synonym="AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1;
                     HEL-S-62p"
                     /note="major polyA site"
ORIGIN      
        1 actcctcccc atcctctccc tctgtccctc tgtccctctg accctgcact gtcccagcac
       61 catgggaccc acctcaggtc ccagcctgct gctcctgcta ctaacccacc tccccctggc
      121 tctggggagt cccatgtact ctatcatcac ccccaacatc ttgcggctgg agagcgagga
      181 gaccatggtg ctggaggccc acgacgcgca aggggatgtt ccagtcactg ttactgtcca
      241 cgacttccca ggcaaaaaac tagtgctgtc cagtgagaag actgtgctga cccctgccac
      301 caaccacatg ggcaacgtca ccttcacgat cccagccaac agggagttca agtcagaaaa
      361 ggggcgcaac aagttcgtga ccgtgcaggc caccttcggg acccaagtgg tggagaaggt
      421 ggtgctggtc agcctgcaga gcgggtacct cttcatccag acagacaaga ccatctacac
      481 ccctggctcc acagttctct atcggatctt caccgtcaac cacaagctgc tacccgtggg
      541 ccggacggtc atggtcaaca ttgagaaccc ggaaggcatc ccggtcaagc aggactcctt
      601 gtcttctcag aaccagcttg gcgtcttgcc cttgtcttgg gacattccgg aactcgtcaa
      661 catgggccag tggaagatcc gagcctacta tgaaaactca ccacagcagg tcttctccac
      721 tgagtttgag gtgaaggagt acgtgctgcc cagtttcgag gtcatagtgg agcctacaga
      781 gaaattctac tacatctata acgagaaggg cctggaggtc accatcaccg ccaggttcct
      841 ctacgggaag aaagtggagg gaactgcctt tgtcatcttc gggatccagg atggcgaaca
      901 gaggatttcc ctgcctgaat ccctcaagcg cattccgatt gaggatggct cgggggaggt
      961 tgtgctgagc cggaaggtac tgctggacgg ggtgcagaac ccccgagcag aagacctggt
     1021 ggggaagtct ttgtacgtgt ctgccaccgt catcttgcac tcaggcagtg acatggtgca
     1081 ggcagagcgc agcgggatcc ccatcgtgac ctctccctac cagatccact tcaccaagac
     1141 acccaagtac ttcaaaccag gaatgccctt tgacctcatg gtgttcgtga cgaaccctga
     1201 tggctctcca gcctaccgag tccccgtggc agtccagggc gaggacactg tgcagtctct
     1261 aacccaggga gatggcgtgg ccaaactcag catcaacaca caccccagcc agaagccctt
     1321 gagcatcacg gtgcgcacga agaagcagga gctctcggag gcagagcagg ctaccaggac
     1381 catgcaggct ctgccctaca gcaccgtggg caactccaac aattacctgc atctctcagt
     1441 gctacgtaca gagctcagac ccggggagac cctcaacgtc aacttcctcc tgcgaatgga
     1501 ccgcgcccac gaggccaaga tccgctacta cacctacctg atcatgaaca agggcaggct
     1561 gttgaaggcg ggacgccagg tgcgagagcc cggccaggac ctggtggtgc tgcccctgtc
     1621 catcaccacc gacttcatcc cttccttccg cctggtggcg tactacacgc tgatcggtgc
     1681 cagcggccag agggaggtgg tggccgactc cgtgtgggtg gacgtcaagg actcctgcgt
     1741 gggctcgctg gtggtaaaaa gcggccagtc agaagaccgg cagcctgtac ctgggcagca
     1801 gatgaccctg aagatagagg gtgaccacgg ggcccgggtg gtactggtgg ccgtggacaa
     1861 gggcgtgttc gtgctgaata agaagaacaa actgacgcag agtaagatct gggacgtggt
     1921 ggagaaggca gacatcggct gcaccccggg cagtgggaag gattacgccg gtgtcttctc
     1981 cgacgcaggg ctgaccttca cgagcagcag tggccagcag accgcccaga gggcagaact
     2041 tcagtgcccg cagccagccg cccgccgacg ccgttccgtg cagctcacgg agaagcgaat
     2101 ggacaaagtc ggcaagtacc ccaaggagct gcgcaagtgc tgcgaggacg gcatgcggga
     2161 gaaccccatg aggttctcgt gccagcgccg gacccgtttc atctccctgg gcgaggcgtg
     2221 caagaaggtc ttcctggact gctgcaacta catcacagag ctgcggcggc agcacgcgcg
     2281 ggccagccac ctgggcctgg ccaggagtaa cctggatgag gacatcattg cagaagagaa
     2341 catcgtttcc cgaagtgagt tcccagagag ctggctgtgg aacgttgagg acttgaaaga
     2401 gccaccgaaa aatggaatct ctacgaagct catgaatata tttttgaaag actccatcac
     2461 cacgtgggag attctggctg tgagcatgtc ggacaagaaa gggatctgtg tggcagaccc
     2521 cttcgaggtc acagtaatgc aggacttctt catcgacctg cggctaccct actctgttgt
     2581 tcgaaacgag caggtggaaa tccgagccgt tctctacaat taccggcaga accaagagct
     2641 caaggtgagg gtggaactac tccacaatcc agccttctgc agcctggcca ccaccaagag
     2701 gcgtcaccag cagaccgtaa ccatcccccc caagtcctcg ttgtccgttc catatgtcat
     2761 cgtgccgcta aagaccggcc tgcaggaagt ggaagtcaag gctgctgtct accatcattt
     2821 catcagtgac ggtgtcagga agtccctgaa ggtcgtgccg gaaggaatca gaatgaacaa
     2881 aactgtggct gttcgcaccc tggatccaga acgcctgggc cgtgaaggag tgcagaaaga
     2941 ggacatccca cctgcagacc tcagtgacca agtcccggac accgagtctg agaccagaat
     3001 tctcctgcaa gggaccccag tggcccagat gacagaggat gccgtcgacg cggaacggct
     3061 gaagcacctc attgtgaccc cctcgggctg cggggaacag aacatgatcg gcatgacgcc
     3121 cacggtcatc gctgtgcatt acctggatga aacggagcag tgggagaagt tcggcctaga
     3181 gaagcggcag ggggccttgg agctcatcaa gaaggggtac acccagcagc tggccttcag
     3241 acaacccagc tctgcctttg cggccttcgt gaaacgggca cccagcacct ggctgaccgc
     3301 ctacgtggtc aaggtcttct ctctggctgt caacctcatc gccatcgact cccaagtcct
     3361 ctgcggggct gttaaatggc tgatcctgga gaagcagaag cccgacgggg tcttccagga
     3421 ggatgcgccc gtgatacacc aagaaatgat tggtggatta cggaacaaca acgagaaaga
     3481 catggccctc acggcctttg ttctcatctc gctgcaggag gctaaagata tttgcgagga
     3541 gcaggtcaac agcctgccag gcagcatcac taaagcagga gacttccttg aagccaacta
     3601 catgaaccta cagagatcct acactgtggc cattgctggc tatgctctgg cccagatggg
     3661 caggctgaag gggcctcttc ttaacaaatt tctgaccaca gccaaagata agaaccgctg
     3721 ggaggaccct ggtaagcagc tctacaacgt ggaggccaca tcctatgccc tcttggccct
     3781 actgcagcta aaagactttg actttgtgcc tcccgtcgtg cgttggctca atgaacagag
     3841 atactacggt ggtggctatg gctctaccca ggccaccttc atggtgttcc aagccttggc
     3901 tcaataccaa aaggacgccc ctgaccacca ggaactgaac cttgatgtgt ccctccaact
     3961 gcccagccgc agctccaaga tcacccaccg tatccactgg gaatctgcca gcctcctgcg
     4021 atcagaagag accaaggaaa atgagggttt cacagtcaca gctgaaggaa aaggccaagg
     4081 caccttgtcg gtggtgacaa tgtaccatgc taaggccaaa gatcaactca cctgtaataa
     4141 attcgacctc aaggtcacca taaaaccagc accggaaaca gaaaagaggc ctcaggatgc
     4201 caagaacact atgatccttg agatctgtac caggtaccgg ggagaccagg atgccactat
     4261 gtctatattg gacatatcca tgatgactgg ctttgctcca gacacagatg acctgaagca
     4321 gctggccaat ggtgttgaca gatacatctc caagtatgag ctggacaaag ccttctccga
     4381 taggaacacc ctcatcatct acctggacaa ggtctcacac tctgaggatg actgtctagc
     4441 tttcaaagtt caccaatact ttaatgtaga gcttatccag cctggagcag tcaaggtcta
     4501 cgcctattac aacctggagg aaagctgtac ccggttctac catccggaaa aggaggatgg
     4561 aaagctgaac aagctctgcc gtgatgaact gtgccgctgt gctgaggaga attgcttcat
     4621 acaaaagtcg gatgacaagg tcaccctgga agaacggctg gacaaggcct gtgagccagg
     4681 agtggactat gtgtacaaga cccgactggt caaggttcag ctgtccaatg actttgacga
     4741 gtacatcatg gccattgagc agaccatcaa gtcaggctcg gatgaggtgc aggttggaca
     4801 gcagcgcacg ttcatcagcc ccatcaagtg cagagaagcc ctgaagctgg aggagaagaa
     4861 acactacctc atgtggggtc tctcctccga tttctgggga gagaagccca acctcagcta
     4921 catcatcggg aaggacactt gggtggagca ctggcccgag gaggacgaat gccaagacga
     4981 agagaaccag aaacaatgcc aggacctcgg cgccttcacc gagagcatgg ttgtctttgg
     5041 gtgccccaac tgaccacacc cccattcccc cactccagat aaagcttcag ttatatctca
     5101 cgtgtctgga gttctttgcc aagagggaga ggctgaaatc cccagccgcc tcacctgcag
     5161 ctcagctcca tcctacttga aacctcacct gttcccaccg cattttctcc tggcgttcgc
     5221 ctgctagtgt g
//
