LOCUS       NM_024093               4587 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens chromosome 2 open reading frame 49 (C2orf49),
            transcript variant 1, mRNA.
ACCESSION   NM_024093
VERSION     NM_024093.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 4587)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   4  (bases 1 to 4587)
  AUTHORS   Popow,J., Jurkin,J., Schleiffer,A. and Martinez,J.
  TITLE     Analysis of orthologous groups reveals archease and DDX1 as tRNA
            splicing factors
  JOURNAL   Nature 511 (7507), 104-107 (2014)
   PUBMED   24870230
REFERENCE   5  (bases 1 to 4587)
  AUTHORS   Popow,J., Englert,M., Weitzer,S., Schleiffer,A., Mierzwa,B.,
            Mechtler,K., Trowitzsch,S., Will,C.L., Luhrmann,R., Soll,D. and
            Martinez,J.
  TITLE     HSPC117 is the essential subunit of a human tRNA splicing ligase
            complex
  JOURNAL   Science 331 (6018), 760-764 (2011)
   PUBMED   21311021
REFERENCE   6  (bases 1 to 4587)
  AUTHORS   Tegha-Dunghu,J., Neumann,B., Reber,S., Krause,R., Erfle,H.,
            Walter,T., Held,M., Rogers,P., Hupfeld,K., Ruppert,T., Ellenberg,J.
            and Gruss,O.J.
  TITLE     EML3 is a nuclear microtubule-binding protein required for the
            correct alignment of chromosomes in metaphase
  JOURNAL   J Cell Sci 121 (Pt 10), 1718-1726 (2008)
   PUBMED   18445686
REFERENCE   7  (bases 1 to 4587)
  AUTHORS   Patil,S.S., Alexander,T.B., Uzman,J.A., Lou,C.H., Gohil,H. and
            Sater,A.K.
  TITLE     Novel gene ashwin functions in Xenopus cell survival and
            anteroposterior patterning
  JOURNAL   Dev Dyn 235 (7), 1895-1907 (2006)
   PUBMED   16680723
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC012360.7, BC001310.1 and BQ003369.1.
            
            On Nov 24, 2018 this sequence version replaced NM_024093.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.218130.1,
                                           SRR1660807.233940.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258457.7/ ENSP00000258457.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC012360.7         33432-33447         c
            17-903              BC001310.1         1-887
            904-3933            AC012360.7         22430-25459         c
            3934-4190           BQ003369.1         18-274              c
            4191-4587           AC012360.7         21776-22172         c
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q12.2"
     gene            1..4587
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /note="chromosome 2 open reading frame 49"
                     /db_xref="GeneID:79074"
                     /db_xref="HGNC:HGNC:28772"
     exon            1..147
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..747
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ashwin"
                     /codon_start=1
                     /product="ashwin isoform 1"
                     /protein_id="NP_076998.1"
                     /db_xref="CCDS:CCDS2068.1"
                     /db_xref="GeneID:79074"
                     /db_xref="HGNC:HGNC:28772"
                     /translation="MAGDVGGRSCTDSELLLHPELLSQEFLLLTLEQKNIAVETDVRV
                     NKDSLTDLYVQHAIPLPQRDLPKNRWGKMMEKKREQHEIKNETKRSSTVDGLRKRPLI
                     VFDGSSTSTSIKVKKTENGDNDRLKPPPQASFTSNAFRKLSNSSSSVSPLILSSNLPV
                     NNKTEHNNNDAKQNHDLTHRKSPSGPVKSPPLSPVGTTPVKLKRAAPKEEAEAMNNLK
                     PPQAKRKIQHVTWP"
     exon            148..314
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /inference="alignment:Splign:2.1.0"
     exon            315..690
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /inference="alignment:Splign:2.1.0"
     exon            691..4587
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /inference="alignment:Splign:2.1.0"
     regulatory      878..883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /note="hexamer: AATAAA"
     polyA_site      900
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /note="major polyA site"
     regulatory      4121..4126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /note="hexamer: AATAAA"
     polyA_site      4139
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /note="major polyA site"
     regulatory      4163..4168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /note="hexamer: ATTAAA"
     polyA_site      4187
                     /gene="C2orf49"
                     /gene_synonym="asw"
     regulatory      4568..4573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C2orf49"
                     /gene_synonym="asw"
                     /note="hexamer: ATTAAA"
     polyA_site      4587
                     /gene="C2orf49"
                     /gene_synonym="asw"
ORIGIN      
        1 cttcgcgggg ctttgtgggt agccgactgg ggtctcctgg cgacgaccat ggcgggggat
       61 gtgggcggtc gcagctgcac ggactcggaa ctgctgctgc acccggagct gctgtcccag
      121 gagttccttc tcctcactct ggagcagaag aacatagctg ttgaaactga tgtaagagta
      181 aacaaagaca gtcttactga cctttatgtc caacatgcaa taccattgcc tcagagggat
      241 ttgccgaaga atagatgggg gaaaatgatg gaaaagaaaa gagaacaaca tgagattaaa
      301 aatgagacta aaaggagtag cactgtagat gggttaagga aaagacccct catcgtattt
      361 gatggaagtt caacaagtac aagcataaaa gtgaaaaaga cagagaatgg agataatgat
      421 cgactgaagc ctcccccgca ggcaagcttt accagtaatg cctttagaaa attatcaaat
      481 tcctcttcga gtgtttcacc cctaattttg tcttccaatt tgcctgtgaa caataaaacg
      541 gaacacaata ataatgacgc taaacagaac catgacttaa cgcataggaa aagtccttca
      601 ggccctgtga agtcgccacc attgtcccct gttggaacta ctccagtgaa gttaaagaga
      661 gctgctccta aagaagaggc agaggccatg aataacctga agcccccaca agcaaaaagg
      721 aagatacaac atgttacttg gccctgaaga aaagtttcca aaaatgtaaa tatactgtaa
      781 ctgtagtttt tcaaatatgt tcatatatat tgacaatatt tacagaaatc ctgattattg
      841 tggaattttc ttaagaggtt tcaaataggt ttaaaaaaat aaaggattta ttttccttcc
      901 cttcttccct ccctcccttc cttttttaaa attcttgcct gtcttgccct gattaggaaa
      961 gaatatcttt ttaaaccaat ggcttagtat atgtcattta tattgaccct actgaaatta
     1021 ttagctacaa atgtgctata aagcatccat tgaattggcc aggcgcagtg gctcacgcct
     1081 ataatcccag cactttgaga gactgaggtg ggcagatcac ctgaggtcag gagtttgaga
     1141 ccagcctgac caacatggag aaaccccgtc tctactaaaa aatacaaaat tagctgggca
     1201 tagtggtgca tgcctgtaat cccagctatt caggaggctg agacaggaga atcgcttgaa
     1261 cctgagaggc ggaggttgcg gggagccaag atcgcaccat tgcactccag cctgggcaac
     1321 aagagcgaaa ctccatctca aaaaaaaaaa agcattcagt gaattttcgg agttactctc
     1381 attagccttg ttcagagtct ttgggggaaa tttgagattt ttgagatttt ttttaaaaac
     1441 tcaaatattt tactagtttg cctgccattt tatttctttt acaaagcaga agcatatacc
     1501 aatttatcac agtattttag taaatactgc aacattcatc cttaaatgtt caccaagaaa
     1561 agcatctttg tagtagtgct ggaaaactat tcagaatata cagataaaaa tgctgttctt
     1621 taattgctta cattgcttct tcccataaaa agcaaaaagg aatcagtgct tgctattgct
     1681 cctttccttg aagttgtaac aattgataca tatattatga gttgactggt cgattctgta
     1741 cctggcccat cctttagaat gttcttgtca tgtagcagtc ctacgtactc ttttcatgag
     1801 cagtctgtga tctcactctg tgagttcagc tattactcgc tcgtgggagc ttaatctttt
     1861 caaaatgaag ttgatttaaa aagtcttcag gcagagtaat catgttagag gtggtattcg
     1921 atggaagaaa gtttagagag ttaggagtgg gggtagaatt ctagaattta taagagtcca
     1981 ggaagcatag cagtcagggg caaaaattag cgtaatatgg agtaggcaat agaggagcta
     2041 ctggagtcag aagtcactgc agagtgcaac ataggaagat ggactcctag cttacatgag
     2101 attccctgca gctgtaatat agacaattcc cacatggctg ttctacacag aattacctgc
     2161 taagattttt tgtttatttt tgtttgagtg gtattttcac tccaattgta taatggaaat
     2221 cagtgggaaa atagggttta ccttatattc atgagttcta gtttctactg ttctgctatg
     2281 tgtttctaag caagagcaaa ggatacttca tacttttttc gttatatgat tgatcttcaa
     2341 attgggattt acctttttca atatgtttta aagtagtctt attcctcttt tgatttgtta
     2401 aacaagcatt ttagttcagc tattgaatag ccttccaaaa aattaattca gccttgcagg
     2461 taagtaccat actaagactt taacccaata gtttttaatc attctgcctt tattccaaac
     2521 tgtaaatctg tacacataag ataaaacata ctaagtattg cataaattgt taacgttaca
     2581 gtaaattgtt atctgcaggg ctgacagaca taatgttggt gggcaactgt gatcctatac
     2641 atacatatat gcaaaagggg attttaaaag tgcagattat agagtagatt gacaaatttt
     2701 attttatatt cagttgtcct ctctgcttcc atctgtgttg ctctcttagt tgagagagag
     2761 ttagccattt gacgatttta agtcagtggg aacttatttt tagttactca ataaaattaa
     2821 tattttattt gtattttaac ttacagagta ggttggtaat aacagctgaa ctgtgtaaca
     2881 ttgttgcttc aaattgaagt ttatattatg aacattcaga atcaatgctc atgtagcagc
     2941 atattattga gctattttga gtttgaaatg tggagaaacg ctaaaccatg tactatgtgt
     3001 taacataatc ccaccttctt agagctttgt tccttctgaa ggtgtataga tacagcttgt
     3061 cttgaaatgt ctttctccac ataatgaagc atgctgaatg ctgggaatct ggagcagcag
     3121 ccctgggagc cctgagtttt gaagtgtttt ggtttgcttc aaaggttaga agaacttgat
     3181 atgtatggca aacaacttta gaatactagt tactcactaa catgaggcgg gtaatgttgc
     3241 tctagattct atattccagt aaagccagct tttcttatta ttggagtagg caaatgaatg
     3301 gcattagaat tagtgggtgg cttgtaagtt gtagttatag gcactttacc acttcctgcc
     3361 attagcaggc atccttgttt tttcttttcc ctctttgttc cttcttttcc ctttctcctt
     3421 atacattttc tttctctact ttaattctcc ttcctcctta ctgtagatcc caagcttcta
     3481 gcttaggttt gcaagtcata ttgcttggcc ctccacattc actgagaggt gaagataggc
     3541 tgaccccctg tcctcttaca tttgagggat catagactgc tgtgtgaatt ctggaaagtc
     3601 tcaggtccct accagggcac tgaatggctt ctcaatggct gtagagacag tacagttttc
     3661 caaagcagcc taattcatct ggacagctac caggcacttt ggaaagttgg ttcagttact
     3721 actatgaggc cataatatat ttgctggtat taaaattctt cagaattgga attactattt
     3781 gaaataatat tttggttgac ttaagttttg agagacaatt ctaaaattga tctagagact
     3841 cattcaatag caatgtgacc ttttaaatac ttacattaag taaaactgcc agtagattaa
     3901 atcatatata tatatatata tatatatata tatgtaagag cttcctctat ttactactgt
     3961 tgaacttcag taatttttag aggctaaata atggtcagaa tgtttttaag tgtgctcttt
     4021 tattacatgc ttgtgcaggt tttgtaattc agtacagaaa agtttaacct tgtacatttt
     4081 tgtatgtaaa aagtctttta agtagtctta tccttattta aataaacaga ataaaattac
     4141 cttgagtagg tctgttattc ttattaaaat ggaaaaatgc tctgtaatga cttgatctgt
     4201 ttttatttga gtgaacaatt ttggaaagta ttctttatag tacaactttc tatacctgga
     4261 ttgattaaga tcagatgtga ttcgagtagt ccagccatat cttgtagccc ttctttgaat
     4321 gagagggtgg ctggagtggt ctggtgctgg gatatcacgg tgctacagag cctgacatgt
     4381 tgactgtcac tacatgttga gggatggaaa tagaagtctc tgaacttccc atgtaatatt
     4441 aaagctctta acaaaatgag acaaactaga gattcagttg agagatttta tgttagagtg
     4501 atctgaaaaa aagttaattt ctaaactgct atcttaatat tattatattt ggagactgat
     4561 gctgtaaatt aaataacttg gaaaaga
//
