LOCUS       NM_032561               1942 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens chromosome 22 open reading frame 23 (C22orf23),
            transcript variant 1, mRNA.
ACCESSION   NM_032561
VERSION     NM_032561.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1942)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1942)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 1942)
  AUTHORS   Collins,J.E., Wright,C.L., Edwards,C.A., Davis,M.P., Grinham,J.A.,
            Cole,C.G., Goward,M.E., Aguado,B., Mallya,M., Mokrab,Y.,
            Huckle,E.J., Beare,D.M. and Dunham,I.
  TITLE     A genome annotation-driven approach to cloning the human ORFeome
  JOURNAL   Genome Biol 5 (10), R84 (2004)
   PUBMED   15461802
REFERENCE   6  (bases 1 to 1942)
  AUTHORS   Shoemaker,D.D., Schadt,E.E., Armour,C.D., He,Y.D.,
            Garrett-Engele,P., McDonagh,P.D., Loerch,P.M., Leonardson,A.,
            Lum,P.Y., Cavet,G., Wu,L.F., Altschuler,S.J., Edwards,S., King,J.,
            Tsang,J.S., Schimmack,G., Schelter,J.M., Koch,J., Ziman,M.,
            Marton,M.J., Li,B., Cundiff,P., Ward,T., Castle,J., Krolewski,M.,
            Meyer,M.R., Mao,M., Burchard,J., Kidd,M.J., Dai,H., Phillips,J.W.,
            Linsley,P.S., Stoughton,R., Scherer,S. and Boguski,M.S.
  TITLE     Experimental annotation of the human genome using microarray
            technology
  JOURNAL   Nature 409 (6822), 922-927 (2001)
   PUBMED   11237012
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI463610.1, BC031998.1, BP368870.1, AF324466.1 and DA968042.1.
            
            On May 17, 2019 this sequence version replaced NM_032561.4.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.227788.1,
                                           SRR5189667.79052.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968189,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000403305.6/ ENSP00000384667.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                BI463610.1         52-123
            73-558              BC031998.1         1-486
            559-652             BP368870.1         488-581
            653-1263            BC031998.1         581-1191
            1264-1466           AF324466.1         1110-1312
            1467-1942           DA968042.1         1-476               c
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.1"
     gene            1..1942
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /note="chromosome 22 open reading frame 23"
                     /db_xref="GeneID:84645"
                     /db_xref="HGNC:HGNC:18589"
                     /db_xref="MIM:619678"
     exon            1..154
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /inference="alignment:Splign:2.1.0"
     exon            155..266
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..817
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPF0193 protein EVG1"
                     /codon_start=1
                     /product="UPF0193 protein EVG1 isoform 1"
                     /protein_id="NP_115950.3"
                     /db_xref="CCDS:CCDS13962.1"
                     /db_xref="GeneID:84645"
                     /db_xref="HGNC:HGNC:18589"
                     /db_xref="MIM:619678"
                     /translation="MASQKQMEVVTKGTGFRRRPKTITYTPGTCELLRVMMKESKLTN
                     IQQRHIMDIMKRGDALPLQCSPTSSQRVLPSKQIASPIYLPPILAARPHLRPANMCQA
                     NGAYSREQFKPQATRDLEKEKQRLQNIFATGKDMEERKRKAPPARQKAPAPELDRFEE
                     LVKEIQERKEFLADMEALGQGKQYRGIILAEISQKLREMEDIDHRRSEELRKGLATT"
     exon            267..329
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /inference="alignment:Splign:2.1.0"
     exon            330..512
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /inference="alignment:Splign:2.1.0"
     exon            513..644
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /inference="alignment:Splign:2.1.0"
     exon            645..745
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /inference="alignment:Splign:2.1.0"
     exon            746..1942
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1239..1244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /note="hexamer: AATAAA"
     polyA_site      1263
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /note="major polyA site"
     regulatory      1447..1452
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
                     /note="hexamer: AATAAA"
     polyA_site      1471
                     /gene="C22orf23"
                     /gene_synonym="dJ1039K5.6; EVG1"
ORIGIN      
        1 atagtagcga gaggcgctag ctgccagctc cagcagccca gatcgcgcag tatttctgcg
       61 tggcaccggt gcggatgcgg ctgtaaagca gtggtcaccg cctccgggag cccacttcta
      121 tgtggagagg ggagcaccca gagagggtct taggagtcct cccatggctt cacagaagca
      181 gatggaggta gtgaccaaag gaactgggtt ccggcgccgc cccaagacca tcacttacac
      241 cccggggacc tgcgagctgc tcagagtgat gatgaaggaa tccaaactga cgaacatcca
      301 gcagcgccac atcatggaca tcatgaaaag aggagatgct ttgcccctac agtgcagccc
      361 aacatccagc cagagagtct taccttccaa gcaaatagcc tcgcccatct acctgcctcc
      421 catcctcgca gcccgtcccc acctccggcc tgccaacatg tgtcaagcca atggggccta
      481 cagccgggag cagttcaagc ctcaagccac cagggatttg gagaaggaga aacaaagact
      541 ccaaaatatc tttgccacag ggaaggacat ggaggaacgg aaaagaaagg cccctcctgc
      601 acgacagaag gctccagccc ctgagctaga ccgatttgaa gagctggtga aggaaatcca
      661 ggagaggaaa gaattcctgg ctgacatgga ggccctggga cagggcaaac agtaccgagg
      721 aatcatcctt gctgaaatct cccagaaact ccgggaaatg gaagacattg accacagaag
      781 gagtgaggaa cttaggaagg gtcttgccac cacttaatct gtctccagag acgggcaggg
      841 tagcccaagc tcgaccactg gagtccactc ctctgccagg cagggccatc ctcaggccag
      901 cccatcctac cagccttctg ccacgtcagg tggtggcata gcactgccct gcagtacccc
      961 aaggcatact cagcagccta gcccctgccc cggaatgcag atggagcctc tgagactcac
     1021 agccccgtcc gcagagaagc aaagcccgag catccctact aggacatgtg tctaaaggta
     1081 tccactgggc ctgcttccca ccacttctga cctgtggcct gttcatttag ttttctgaat
     1141 tcaggaatga atatgattcc tttcttcctg ttttaggatt tacttatttg atttcttacc
     1201 gccttaggca agtattcaac cacaatgatt aactggccaa taaaacatat ttaacgtggg
     1261 tcattacaag caccattttg ttttcagagg gataagctta tttgttcaag gcattagact
     1321 ttgtcaagcc cagatggttt ctagctacct gtttgcatat ttcaagtggc aacgggaacc
     1381 aacctcttgg gcaccccatg gctggctgct gtggcgggtg ctgaattcca ctctgttaat
     1441 gatctcaata aacctctgca acggttcatt atccctctgt gaaaactaag tctcaacaag
     1501 ataccacaac caagggcatt tacctggcaa gcagtgcatt caggattaaa agttggcttt
     1561 agctttatgt ctttccacta tacccatggc cacagaaaga aacatgaaaa ggtgggagcg
     1621 acctagagcc ctggcctggg agttccaatc cctgcatgtg ccgcgtgacc tttcttgggc
     1681 aaaacccacc agctccgtgc ttgtgcatct gcgaggccct gctgggaagg agtcacctga
     1741 caccttgtcc tgtacacagc acatctgtcc ccctcccctc tggctcctga cacccacgat
     1801 ccctgccttc tccggtattc tgtcatttgc ttgtcccact aaatcaccaa aatattttgg
     1861 aaatatcaac cttgtctcgg gcacctggag ggagtcccat aatcccatca ggtcacatga
     1921 cccaagtttc ggtttgcaga at
//
