LOCUS       NM_001098514            1360 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens chromosome 16 open reading frame 89 (C16orf89),
            transcript variant 2, mRNA.
ACCESSION   NM_001098514
VERSION     NM_001098514.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Afink,G.B., Veenboer,G., de Randamie,J., Keijser,R., Meischl,C.,
            Niessen,H. and Ris-Stalpers,C.
  TITLE     Initial characterization of C16orf89, a novel thyroid-specific gene
  JOURNAL   Thyroid 20 (7), 811-821 (2010)
   PUBMED   20578903
  REMARK    GeneRIF: characterization of C16orf89; mRNA and translated protein
            sequences (signal peptide, O-glycosylation sites); mRNA expression
            in various tissues primarily thyroid
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC026458.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 20, 2020 this sequence version replaced NM_001098514.2.
            
            Summary: This gene is expressed predominantly in the thyroid. Based
            on expression patterns similar to thyroid transcription factors and
            proteins, this gene may function in the development and function of
            the thyroid. Multiple transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Oct
            2011].
            
            Transcript Variant: This variant (2) uses an alternate acceptor
            splice site at the 3' terminal exon compared to variant 1. This
            results in a frame-shift and a shorter isoform (2) with a distinct
            C-terminus compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC033681.1, AK300708.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: experimental evidence
                                                (PMID:20578903)
            MANE Ensembl match               :: ENST00000472572.8/
                                                ENSP00000420566.2
            RefSeq Select criteria           :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-256               AC026458.8         54108-54363         c
            257-406             AC026458.8         50832-50981         c
            407-557             AC026458.8         48693-48843         c
            558-675             AC026458.8         46900-47017         c
            676-811             AC026458.8         44460-44595         c
            812-916             AC026458.8         43653-43757         c
            917-1003            AC026458.8         36285-36371         c
            1004-1360           AC026458.8         32529-32885         c
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..1360
                     /gene="C16orf89"
                     /note="chromosome 16 open reading frame 89"
                     /db_xref="GeneID:146556"
                     /db_xref="HGNC:HGNC:28687"
     exon            1..256
                     /gene="C16orf89"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..1134
                     /gene="C16orf89"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; UPF0764 protein C16orf89"
                     /codon_start=1
                     /product="UPF0764 protein C16orf89 isoform 2 precursor"
                     /protein_id="NP_001091984.2"
                     /db_xref="CCDS:CCDS45404.2"
                     /db_xref="GeneID:146556"
                     /db_xref="HGNC:HGNC:28687"
                     /translation="MASLGLLLLLLLTALPPLWSSSLPGLDTAESKATIADLILSALE
                     RATVFLEQRLPEINLDGMVGVRVLEEQLKSVREKWAQEPLLQPLSLRVGMLGEKLEAA
                     IQRSLHYLKLSDPKYLREFQLTLQPGFWKLPHAWIHTDASLVYPTFGPQDSFSEERSD
                     VCLVQLLGTGTDSSEPCGLSDLCRSLMTKPGCSGYCLSHQLLFFLWARMRGCTQGPLQ
                     QSQDYINLFCANMMDLNRRAEAIGYAYPTRDIFMENIMFCGMGGFSDFYKLRWLEAIL
                     SWQKQQEGCFGEPDAEDEELSKAIQYQQHFSRRVKRREKQFPDGCSSHNTATAVAALG
                     GFLYILAEYPPANREPHPSTPPPPSSR"
     exon            257..406
                     /gene="C16orf89"
                     /inference="alignment:Splign:2.1.0"
     exon            407..557
                     /gene="C16orf89"
                     /inference="alignment:Splign:2.1.0"
     exon            558..675
                     /gene="C16orf89"
                     /inference="alignment:Splign:2.1.0"
     exon            676..811
                     /gene="C16orf89"
                     /inference="alignment:Splign:2.1.0"
     exon            812..916
                     /gene="C16orf89"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1003
                     /gene="C16orf89"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1360
                     /gene="C16orf89"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1339..1344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C16orf89"
                     /note="hexamer: AATAAA"
     polyA_site      1360
                     /gene="C16orf89"
                     /note="major polyA site"
ORIGIN      
        1 agagctcagg gtgctgagcg tgtgaccagc agtgagcaga ggccggccat ggccagcctg
       61 gggctgctgc tcctgctctt actgacagca ctgccaccgc tgtggtcctc ctcactgcct
      121 gggctggaca ctgctgaaag taaagccacc attgcagacc tgatcctgtc tgcgctggag
      181 agagccaccg tcttcctaga acagaggctg cctgaaatca acctggatgg catggtgggg
      241 gtccgagtgc tggaagagca gctaaaaagt gtccgggaga agtgggccca ggagcccctg
      301 ctgcagccgc tgagcctgcg cgtggggatg ctgggggaga agctggaggc tgccatccag
      361 agatccctcc actacctcaa gctgagtgat cccaagtacc taagagagtt ccagctgacc
      421 ctccagcccg ggttttggaa gctcccacat gcctggatcc acactgatgc ctccttggtg
      481 taccccacgt tcgggcccca ggactcattc tcagaggaga gaagtgacgt gtgcctggtg
      541 cagctgctgg gaaccgggac ggacagcagc gagccctgcg gcctctcaga cctctgcagg
      601 agcctcatga ccaagcccgg ctgctcaggc tactgcctgt cccaccaact gctcttcttc
      661 ctctgggcca gaatgagggg gtgcacacag ggaccactcc aacagagcca ggactatatc
      721 aacctcttct gcgccaacat gatggacttg aaccgcagag ctgaggccat cggatacgcc
      781 taccctaccc gggacatctt catggaaaac atcatgttct gtggaatggg cggcttctcc
      841 gacttctaca agctccggtg gctggaggcc attctcagct ggcagaaaca gcaggaagga
      901 tgcttcgggg agcctgatgc tgaagatgaa gaattatcta aagctattca atatcagcag
      961 catttttcga ggagagtgaa gaggcgagaa aaacaatttc cagatggctg ctcctcccac
     1021 aacacagcca cagcagtggc agccctgggt ggcttcctat acatcctggc agaatacccc
     1081 ccagcaaaca gagagccaca cccatccaca ccgccaccac caagcagccg ctgagacgga
     1141 cggttccatg ccagctgcct ggaggaggaa cagacccctt tagtcctcat cccttagatc
     1201 ctggagggca cggatcacat cctgggaaga aggcatctgg aggataagca aagccacccc
     1261 gacacccaat cttggaagcc ctgagtaggc aaggccaggg taggtggggg ccgggaggga
     1321 cccaggtgtg aacggatgaa taaagttcaa ctgcaactga
//
