LOCUS       NM_152587               1276 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens chromosome 11 open reading frame 65 (C11orf65),
            transcript variant 1, mRNA.
ACCESSION   NM_152587
VERSION     NM_152587.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1276)
  AUTHORS   Lee,J., Pappalardo,Z., Chopra,D.G., Hennings,T.G., Vaughn,I.,
            Lan,C., Choe,J.J., Ang,K., Chen,S., Arkin,M., McManus,M.T.,
            German,M.S. and Ku,G.M.
  TITLE     A Genetic Interaction Map of Insulin Production Identifies Mfi as
            an Inhibitor of Mitochondrial Fission
  JOURNAL   Endocrinology 159 (9), 3321-3330 (2018)
   PUBMED   30059978
REFERENCE   4  (bases 1 to 1276)
  AUTHORS   Florez,J.C.
  TITLE     Does metformin work for everyone? A genome-wide association study
            for metformin response
  JOURNAL   Curr Diab Rep 11 (6), 467-469 (2011)
   PUBMED   21845381
REFERENCE   5  (bases 1 to 1276)
  AUTHORS   Zhou,K., Bellenguez,C., Spencer,C.C., Bennett,A.J., Coleman,R.L.,
            Tavendale,R., Hawley,S.A., Donnelly,L.A., Schofield,C.,
            Groves,C.J., Burch,L., Carr,F., Strange,A., Freeman,C.,
            Blackwell,J.M., Bramon,E., Brown,M.A., Casas,J.P., Corvin,A.,
            Craddock,N., Deloukas,P., Dronov,S., Duncanson,A., Edkins,S.,
            Gray,E., Hunt,S., Jankowski,J., Langford,C., Markus,H.S.,
            Mathew,C.G., Plomin,R., Rautanen,A., Sawcer,S.J., Samani,N.J.,
            Trembath,R., Viswanathan,A.C., Wood,N.W., Harries,L.W.,
            Hattersley,A.T., Doney,A.S., Colhoun,H., Morris,A.D.,
            Sutherland,C., Hardie,D.G., Peltonen,L., McCarthy,M.I.,
            Holman,R.R., Palmer,C.N., Donnelly,P. and Pearson,E.R.
  CONSRTM   GoDARTS and UKPDS Diabetes Pharmacogenetics Study Group; Wellcome
            Trust Case Control Consortium 2; MAGIC investigators
  TITLE     Common variants near ATM are associated with glycemic response to
            metformin in type 2 diabetes
  JOURNAL   Nat Genet 43 (2), 117-120 (2011)
   PUBMED   21186350
REFERENCE   6  (bases 1 to 1276)
  AUTHORS   Guey,L.T., Garcia-Closas,M., Murta-Nascimento,C., Lloreta,J.,
            Palencia,L., Kogevinas,M., Rothman,N., Vellalta,G., Calle,M.L.,
            Marenne,G., Tardon,A., Carrato,A., Garcia-Closas,R., Serra,C.,
            Silverman,D.T., Chanock,S., Real,F.X. and Malats,N.
  CONSRTM   EPICURO/Spanish Bladder Cancer Study investigators
  TITLE     Genetic susceptibility to distinct bladder cancer subphenotypes
  JOURNAL   Eur Urol 57 (2), 283-292 (2010)
   PUBMED   19692168
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB104722.1, BC029536.1 and AP005718.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_152587.4.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC029536.1, SRR14372080.3305387.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393084.6/ ENSP00000376799.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                DB104722.1         3-27
            26-1028             BC029536.1         1-1003
            1029-1276           AP005718.3         58864-59111         c
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q22.3"
     gene            1..1276
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /note="chromosome 11 open reading frame 65"
                     /db_xref="GeneID:160140"
                     /db_xref="HGNC:HGNC:28519"
     exon            1..59
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     exon            60..149
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1010
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /note="isoform 1 is encoded by transcript variant 1;
                     uncharacterized protein C11orf65; mitochondrial fission
                     factor interactor; protein MFI"
                     /codon_start=1
                     /product="protein MFI isoform 1"
                     /protein_id="NP_689800.3"
                     /db_xref="CCDS:CCDS8340.1"
                     /db_xref="GeneID:160140"
                     /db_xref="HGNC:HGNC:28519"
                     /translation="MPWKEESEFTKQDKAARVIQQAWKSFLNVAIFQHFKSLIDLRRQ
                     GEPRQIVKYINPKEAELLDAAAGIHVRFRLGGVKFPPDIYYKIFTHRPIEDLCANSPR
                     NYAKLPAKHTSHNKNDHLQEEDHSGWYHRIENNGWRPVSDTFWLSTDGMVVEDKKESE
                     FHFSKLKRRQDLEKKRKLRKIEWMRQMYYSGSLEAKSTHHETLGLIHTATKGLIRAFE
                     DGGIDSVMEWEVDEVLNWTNTLNFDEYIASWKEIATSNSSANFKGFRFNQAQKNIYNY
                     GGDISKMQMGIPDDTYYENVYQEPNVTRLTPDSTYGL"
     exon            150..242
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     exon            243..296
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     exon            297..497
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     exon            498..628
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     exon            629..799
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     exon            800..855
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1276
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1006..1011
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /note="hexamer: TATAAA"
     polyA_site      1031
                     /gene="C11orf65"
                     /gene_synonym="MFI"
     regulatory      1251..1256
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /note="hexamer: AGTAAA"
     polyA_site      1276
                     /gene="C11orf65"
                     /gene_synonym="MFI"
                     /note="major polyA site"
ORIGIN      
        1 gtcatttccg cctgcgacgt tacctaggag tccagcggcg cccgggccag cagcgattgg
       61 aatttcaaat gccttggaaa gaggaatcag aatttacaaa gcaggataag gctgccagag
      121 tcattcagca ggcctggaaa agtttcctta atgtcgctat atttcaacac tttaaaagtc
      181 tgattgattt aagaagacaa ggagaaccac gtcagatagt gaaatatatt aatcccaaag
      241 aggcagagct tctagatgct gctgctggca ttcatgtgcg attcagatta ggtggagtta
      301 aatttccacc tgatatatac tataagattt ttactcacag acctattgaa gatctctgtg
      361 ctaacagccc tagaaattat gcaaaacttc cagcaaagca tacatctcat aataaaaatg
      421 atcatcttca ggaagaggat catagtggct ggtatcatcg tatagaaaac aatggctgga
      481 ggccagtttc tgatacattt tggctatcta ctgatggtat ggtggtggaa gacaaaaagg
      541 aaagtgaatt ccatttctct aaactgaaga gaaggcaaga tttggaaaag aaaagaaaac
      601 ttagaaaaat agagtggatg aggcaaatgt actactcagg aagtctggag gctaagtcaa
      661 cacatcatga aactctagga ctaattcaca ctgcaacaaa ggggctgatt agagcttttg
      721 aagatggggg gatagattct gtgatggaat gggaagtgga tgaagtgctg aactggacaa
      781 atacactgaa ctttgatgag tacattgcca gctggaagga aattgctaca agcaactctt
      841 cggctaactt caaaggattc aggtttaatc aagcacagaa aaacatatat aactatggag
      901 gagacatatc aaagatgcaa atgggaatac cagatgatac ttactatgaa aatgtttatc
      961 aagaaccaaa tgtaactaga ttaacgcctg attctactta tggactataa agtactttat
     1021 ttctattctg agttatagcc ctttgagcct tccatccatc agttatagcc aagagatagt
     1081 gtattctgac tggaataact tgtgtatata ttctgggcac agaatctagc tcagttaagt
     1141 aaggaagatt gagcagaaat agaacactgg actgaataca atgatcatgg caaatataga
     1201 aattattcct agtattctga gaaactgtag tattctaaca gtcacactta agtaaagaga
     1261 ctacatttag gtgcaa
//
