LOCUS       NM_033637               6024 bp    mRNA    linear   PRI 19-MAR-2025
DEFINITION  Homo sapiens beta-transducin repeat containing E3 ubiquitin protein
            ligase (BTRC), transcript variant 1, mRNA.
ACCESSION   NM_033637 NM_032715
VERSION     NM_033637.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Li,J., Zhao,L., Wu,Z., Huang,S., Wang,J., Chang,Y., Liu,L., Jin,H.,
            Lu,J., Huang,C., Xie,Q., Huang,H. and Su,Z.
  TITLE     SelK promotes glioblastoma cell proliferation by inhibiting
            beta-TrCP1 mediated ubiquitin-dependent degradation of CDK4
  JOURNAL   J Exp Clin Cancer Res 43 (1), 231 (2024)
   PUBMED   39155374
  REMARK    GeneRIF: SelK promotes glioblastoma cell proliferation by
            inhibiting beta-TrCP1 mediated ubiquitin-dependent degradation of
            CDK4.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Shih,Y.C., Chen,H.F., Wu,C.Y., Ciou,Y.R., Wang,C.W., Chuang,H.C.
            and Tan,T.H.
  TITLE     The phosphatase DUSP22 inhibits UBR2-mediated K63-ubiquitination
            and activation of Lck downstream of TCR signalling
  JOURNAL   Nat Commun 15 (1), 532 (2024)
   PUBMED   38225265
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6024)
  AUTHORS   Xiong,M., Wang,Q., Zhang,X., Wen,L. and Zhao,A.
  TITLE     Decidual stromal cells-derived exosomes incurred insufficient
            migration and invasion of trophoblast by disturbing of
            beta-TrCP-mediated snail ubiquitination and degradation in
            unexplained recurrent spontaneous abortion
  JOURNAL   Eur J Med Res 29 (1), 39 (2024)
   PUBMED   38195659
  REMARK    GeneRIF: Decidual stromal cells-derived exosomes incurred
            insufficient migration and invasion of trophoblast by disturbing of
            beta-TrCP-mediated snail ubiquitination and degradation in
            unexplained recurrent spontaneous abortion.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6024)
  AUTHORS   Abdullah, Hussain,S., Ji,W., Khan,H., Mis,E.K., Mushtaq,R.,
            Chodhary,M., Raza,M.H., Jan,A., Ullah,I., Khokha,M.K., Lakhani,S.A.
            and Ahmad,W.
  TITLE     Sequence variants in DLX5, HOXD13 and 445 kb-microduplication
            surrounding BTRC cause split-hand/foot malformation in three
            different families
  JOURNAL   Clin Genet 105 (1), 109-111 (2024)
   PUBMED   37776184
  REMARK    GeneRIF: Sequence variants in DLX5, HOXD13 and 445
            kb-microduplication surrounding BTRC cause split-hand/foot
            malformation in three different families.
REFERENCE   5  (bases 1 to 6024)
  AUTHORS   Sethi,S.C., Shrestha,R.L., Balachandra,V., Durairaj,G., Au,W.C.,
            Nirula,M., Karpova,T.S., Kaiser,P. and Basrai,M.A.
  TITLE     beta-TrCP-Mediated Proteolysis of Mis18beta Prevents
            Mislocalization of CENP-A and Chromosomal Instability
  JOURNAL   Mol Cell Biol 44 (10), 429-442 (2024)
   PUBMED   39135477
  REMARK    GeneRIF: beta-TrCP-Mediated Proteolysis of Mis18beta Prevents
            Mislocalization of CENP-A and Chromosomal Instability.
REFERENCE   6  (bases 1 to 6024)
  AUTHORS   Suzuki,H., Chiba,T., Kobayashi,M., Takeuchi,M., Suzuki,T.,
            Ichiyama,A., Ikenoue,T., Omata,M., Furuichi,K. and Tanaka,K.
  TITLE     IkappaBalpha ubiquitination is catalyzed by an SCF-like complex
            containing Skp1, cullin-1, and two F-box/WD40-repeat proteins,
            betaTrCP1 and betaTrCP2
  JOURNAL   Biochem Biophys Res Commun 256 (1), 127-132 (1999)
   PUBMED   10066435
REFERENCE   7  (bases 1 to 6024)
  AUTHORS   Spencer,E., Jiang,J. and Chen,Z.J.
  TITLE     Signal-induced ubiquitination of IkappaBalpha by the F-box protein
            Slimb/beta-TrCP
  JOURNAL   Genes Dev 13 (3), 284-294 (1999)
   PUBMED   9990853
REFERENCE   8  (bases 1 to 6024)
  AUTHORS   Winston,J.T., Strack,P., Beer-Romero,P., Chu,C.Y., Elledge,S.J. and
            Harper,J.W.
  TITLE     The SCFbeta-TRCP-ubiquitin ligase complex associates specifically
            with phosphorylated destruction motifs in IkappaBalpha and
            beta-catenin and stimulates IkappaBalpha ubiquitination in vitro
  JOURNAL   Genes Dev 13 (3), 270-283 (1999)
   PUBMED   9990852
  REMARK    Erratum:[Genes Dev 1999 Apr 15;13(8):1050]
REFERENCE   9  (bases 1 to 6024)
  AUTHORS   Yaron,A., Hatzubai,A., Davis,M., Lavon,I., Amit,S., Manning,A.M.,
            Andersen,J.S., Mann,M., Mercurio,F. and Ben-Neriah,Y.
  TITLE     Identification of the receptor component of the
            IkappaBalpha-ubiquitin ligase
  JOURNAL   Nature 396 (6711), 590-594 (1998)
   PUBMED   9859996
REFERENCE   10 (bases 1 to 6024)
  AUTHORS   Margottin,F., Bour,S.P., Durand,H., Selig,L., Benichou,S.,
            Richard,V., Thomas,D., Strebel,K. and Benarous,R.
  TITLE     A novel human WD protein, h-beta TrCp, that interacts with HIV-1
            Vpu connects CD4 to the ER degradation pathway through an F-box
            motif
  JOURNAL   Mol Cell 1 (4), 565-574 (1998)
   PUBMED   9660940
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC027994.1, CR749295.1 and
            BC006204.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_033637.3.
            
            Summary: This gene encodes a member of the F-box protein family
            which is characterized by an approximately 40 amino acid motif, the
            F-box. The F-box proteins constitute one of the four subunits of
            ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),
            which function in phosphorylation-dependent ubiquitination. The
            F-box proteins are divided into 3 classes: Fbws containing WD-40
            domains, Fbls containing leucine-rich repeats, and Fbxs containing
            either different protein-protein interaction modules or no
            recognizable motifs. The protein encoded by this gene belongs to
            the Fbws class; in addition to an F-box, this protein contains
            multiple WD-40 repeats. The encoded protein mediates degradation of
            CD4 via its interaction with HIV-1 Vpu. It has also been shown to
            ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light
            polypeptide gene enhancer in B-cells inhibitor, alpha), targeting
            it for degradation and thus activating nuclear factor kappa-B.
            Alternatively spliced transcript variants have been described. A
            related pseudogene exists in chromosome 6. [provided by RefSeq, Mar
            2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.60332.1,
                                           SRR1660809.89042.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370187.8/ ENSP00000359206.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2198              BC027994.1         22-2219
            2199-3122           CR749295.1         2184-3107
            3123-4712           CR749295.1         3109-4698
            4713-4754           BC006204.2         1807-1848
            4755-4755           CR749295.1         4741-4741
            4756-6024           BC006204.2         1850-3118
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.32"
     gene            1..6024
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /note="beta-transducin repeat containing E3 ubiquitin
                     protein ligase"
                     /db_xref="GeneID:8945"
                     /db_xref="HGNC:HGNC:1144"
                     /db_xref="MIM:603482"
     exon            1..64
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1834
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     beta-TrCP1; F-box/WD repeat-containing protein 1A;
                     E3RSIkappaB; pIkappaBalpha-E3 receptor subunit; epididymis
                     tissue protein Li 2a; F-box and WD repeats protein
                     beta-TrCP; F-box and WD-repeat protein 1B"
                     /codon_start=1
                     /product="F-box/WD repeat-containing protein 1A isoform 1"
                     /protein_id="NP_378663.1"
                     /db_xref="CCDS:CCDS7512.1"
                     /db_xref="GeneID:8945"
                     /db_xref="HGNC:HGNC:1144"
                     /db_xref="MIM:603482"
                     /translation="MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPG
                     TGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKT
                     ENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQM
                     CHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRV
                     TSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKII
                     QDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLE
                     CKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFN
                     NGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKV
                     WNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEEL
                     VRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEF
                     QIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR"
     exon            65..172
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            173..250
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            251..340
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            341..572
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            573..759
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            760..856
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..994
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1113
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1363
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1482
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1593
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1672
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1865
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..6024
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2908..2913
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /note="hexamer: AATAAA"
     polyA_site      2935
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /note="major polyA site"
     regulatory      6006..6011
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /note="hexamer: AATATA"
     polyA_site      6024
                     /gene="BTRC"
                     /gene_synonym="BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;
                     FBXW1; FBXW1A; FWD1"
                     /note="major polyA site"
ORIGIN      
        1 agtggcctcg gcgattatgg acccggccga ggcggtgctg caagagaagg cactcaagtt
       61 tatgtgctct atgcccaggt ctctgtggct gggctgctcc agcctggcgg acagcatgcc
      121 ttcgctgcga tgcctgtata acccagggac tggcgcactc acagctttcc agaattcctc
      181 agagagagaa gactgtaata atggcgaacc ccctaggaag ataataccag agaagaattc
      241 acttagacag acatacaaca gctgtgccag actctgctta aaccaagaaa cagtatgttt
      301 agcaagcact gctatgaaga ctgagaattg tgtggccaaa acaaaacttg ccaatggcac
      361 ttccagtatg attgtgccca agcaacggaa actctcagca agctatgaaa aggaaaagga
      421 actgtgtgtc aaatactttg agcagtggtc agagtcagat caagtggaat ttgtggaaca
      481 tcttatatcc caaatgtgtc attaccaaca tgggcacata aactcgtatc ttaaacctat
      541 gttgcagaga gatttcataa ctgctctgcc agctcgggga ttggatcata ttgctgagaa
      601 cattctgtca tacctggatg ccaaatcact atgtgctgct gaacttgtgt gcaaggaatg
      661 gtaccgagtg acctctgatg gcatgctgtg gaagaagctt atcgagagaa tggtcaggac
      721 agattctctg tggagaggcc tggcagaacg aagaggatgg ggacagtatt tattcaaaaa
      781 caaacctcct gacgggaatg ctcctcccaa ctctttttat agagcacttt atcctaaaat
      841 tatacaagac attgagacaa tagaatctaa ttggagatgt ggaagacata gtttacagag
      901 aattcactgc cgaagtgaaa caagcaaagg agtttactgt ttacagtatg atgatcagaa
      961 aatagtaagc ggccttcgag acaacacaat caagatctgg gataaaaaca cattggaatg
     1021 caagcgaatt ctcacaggcc atacaggttc agtcctctgt ctccagtatg atgagagagt
     1081 gatcataaca ggatcatcgg attccacggt cagagtgtgg gatgtaaata caggtgaaat
     1141 gctaaacacg ttgattcacc attgtgaagc agttctgcac ttgcgtttca ataatggcat
     1201 gatggtgacc tgctccaaag atcgttccat tgctgtatgg gatatggcct ccccaactga
     1261 cattaccctc cggagggtgc tggtcggaca ccgagctgct gtcaatgttg tagactttga
     1321 tgacaagtac attgtttctg catctgggga tagaactata aaggtatgga acacaagtac
     1381 ttgtgaattt gtaaggacct taaatggaca caaacgaggc attgcctgtt tgcagtacag
     1441 ggacaggctg gtagtgagtg gctcatctga caacactatc agattatggg acatagaatg
     1501 tggtgcatgt ttacgagtgt tagaaggcca tgaggaattg gtgcgttgta ttcgatttga
     1561 taacaagagg atagtcagtg gggcctatga tggaaaaatt aaagtgtggg atcttgtggc
     1621 tgctttggac ccccgtgctc ctgcagggac actctgtcta cggacccttg tggagcattc
     1681 cggaagagtt tttcgactac agtttgatga attccagatt gtcagtagtt cacatgatga
     1741 cacaatcctc atctgggact tcctaaatga tccagctgcc caagctgaac ccccccgttc
     1801 cccttctcga acatacacct acatctccag ataaataacc atacactgac ctcatacttg
     1861 cccaggaccc attaaagttg cggtatttaa cgtatctgcc aataccagga tgagcaacaa
     1921 cagtaacaat caaactactg cccagtttcc ctggactagc cgaggagcag ggctttgaga
     1981 ctcctgttgg gacacagttg gtctgcagtc ggcccaggac ggtctactca gcacaactga
     2041 ctgcttcagt gctgctatca gaagatgtct tctatctttt gtgaatgatt ggaactttta
     2101 aacctcccct cctctcctcc tttcacctct gcacctagtt ttttcccatt ggttccagac
     2161 aaaggtgact tataaatata tttagtgttt tgccagaatc tctcttgctt tgccattaag
     2221 cagaagaact agtttccctg tatagcctgc tgggagagac ccacttctag ggtatggggg
     2281 atgcagcttc aagcccagtg cccagtgtct ccctgttaac tgcaggaatg ccaagcacct
     2341 ggccagagca gcccagcccc aatatgctta ggaggagaca gagttccctc tgtatagcct
     2401 ctgggacaag aaaaagaaaa cacaagaatg tatacactgg aagatttggg cctcctgcct
     2461 gccttctctt tgtttctgtt cctcttccca tctactcccc tacgcccctt caaccttttt
     2521 tctctgtctg cttcacctga gaagaaagtg tacgaagaga gtgtcctcct ctcacatgag
     2581 ccagatcagc cagaaaatgc aacacttgga agagttaaat gctgttcagt gaagatttca
     2641 gccccaggcc tttgctgcaa gtgaccctgt ggcaacagtg gattctcaga catgatactc
     2701 tcatcatatt tgcaactctt ctctctcttt cttccccaca cccaagagga ggattggtgg
     2761 tagggggcag gcagaggggg tggggagaag tttcctgggc tccatcaatg gctgcatctt
     2821 ttctggactc agcagtctcc ttgattccat gtagagtgtg gaaaggagtt gctgattgca
     2881 tttcctctca ttaacaattg ggtgtgtaat aaaaagcatt gtacttcatc ttaaatcact
     2941 ggtaaggctc agcctacaga aagatttgaa atggccagag ccaatcgctt ggtgcattct
     3001 gcgtaatggt ttccatctcc gatttcctca tcagggcctg tgaataccca ggtgcctgta
     3061 tctttgccaa gaccgtgatc aaggtagctt aagagagatg gtcaggagaa aacactgttt
     3121 ttgttttttt tgttgttttg ttttgttttg gccagttaaa tatcatctct caaatattga
     3181 tctcaccgtg tcaaccttgc actgcacaac cttccttctg cttctcccac acccagtatt
     3241 tgcagaaggg caaagctgct taagagagag gatcagggtg aagtttggca cacagggttt
     3301 attaatgggg caaaaactgc cttttcttcc tcctcctgac cttattttgc tcttcactct
     3361 ccccagccaa taaagcgtct gtggcgattg gtgaacagca taaacagctg gacctcagca
     3421 agggtcaggc aaacccagtc actcggaagg cagctgtgtg agctgccaag ctagtgggct
     3481 tcaggtgcaa gggtacctgt gccacaccaa cctgggagca cacagaatac tattaatgtg
     3541 cacccagctg gtctccccag gcaagaaggt atcctcttcc caaggtgtac ccactgaatg
     3601 ttgttactac atattgagag tcattttatg catatgcatt ctacctttcc tgctttatga
     3661 gtatttttaa gcttttagtt caaggttata ttcagaaaat atttcccagt ataatgatac
     3721 atcgtagcct aagaaatatt ttctcaatgt aattcccttc ccagctaccc aaatgctaca
     3781 gagaaatgtt ttctacttgg ccactatcag ggttcgtcat ctattgtgtt gactattaat
     3841 ggctttttga ttgggtaagg attttgctat agatgaaggt agagggctgt cagccctgaa
     3901 aaacacacag gtcagacatt taaaaggcat gggtttcgag ctgtctcaaa atattgccca
     3961 atagccataa ttttaccagc ctttctgtca tatgctgcta ttacaaagtg gaagctgttg
     4021 aatgtttatt ggtgcccagg gttttgctct ccaatctagg ttcagttgaa ggaatattgt
     4081 ttctaagact gttttgagac atgtccagta catcacaaag gagatcgggg cgacccctgc
     4141 agatgtggag ccattagccc agttgaggat attctccaag ttgtcctctc tcctgctgat
     4201 ggaaatggga atgaagttaa gtggtctgaa aaacttgaat cgttcacatt tctcagctct
     4261 gggggtcatt taccagtttg ttgtagaaga aataatcagg taagttaaaa gttcatttcc
     4321 agagaaggta aaccccactt accatctctg catgatttca gtgggaattg attatcacta
     4381 atccccaact gggctagaat aaatgtaaag tttgaccttt ttaaaacgaa aagagagaca
     4441 aagtctcagc acattccaag gagtggtaga aacagagctg aaggtgtccc cattgtagat
     4501 tagtctcttc tcactaaaat ttactttcca acgtagggcc taaaggaaac ctttcttaaa
     4561 gacaggctga aaccccttca aaggcagatg aggaggtaca gacacgtgac cttttggtgc
     4621 acactggagc tacttggaca agaccagcat gccttgctgc acgtgtgtgt atttcactgc
     4681 tgagaacatc ctttaacttg gtgtgcaatt tgaaaggatg tgaatcatgg atggaaggcc
     4741 atttgtacat gtcccttggc aaaattcttt ctggtgtctc ctaacttcag agacagggac
     4801 tctttttgga tctctattga caagtaataa aagtctggcc ctcataactt gtttccgaac
     4861 tagaaaagtc tgtgagaccc ctacatcatt ctggtttttt tgcttgagta agaacaatcc
     4921 ttttttattt ttcttctgta cagtctaaag ctacagagaa aaaaaaatgc actcttccct
     4981 tgccggctcc tggtaccatt ggtctgaaca gctgtagttg gtctactcct tacttagcac
     5041 ttgattgtgt ggggaaacaa aggtgggagg ggtggggaat actggaaata atcagggcaa
     5101 tttttttctt tcccataatt ggactagata ccttggtact gttgaccttc tcagcatctc
     5161 ccttttgcct tagatggcaa caccctccag tctgtagcag agcagtccaa cccagattag
     5221 tgcagcccgg aggcttaggg tgcagcctcc ctggtcttcc tccacacagt tgttcaccaa
     5281 cagaccagac ctcctttaac cacagtgtca acatagtatc ggaaagagag ccatttctta
     5341 ggggaataaa acagtttcgc ttctttagct catctgtggt gtcagaatcc ttggagctga
     5401 agagagaaat caaaagagca tgatgatggc tgcctggttt caggtggaac ttaatgcatt
     5461 gatctttaga agctccttct gttggaagtt gagtacctgt gatctaaaat gtcctggagg
     5521 cagatgacat ctaaaatatg tgctttccaa ccagcacagc tggcgctctt agctcctgat
     5581 tggttgtgtg ttttattaag gatcagtgca gttaagtcgt attttaaagt gttacctccc
     5641 ctcctaaccc ttccccttct tggacactga aggaaaaggc caactagggt gttagccctc
     5701 tgggcaccaa ggaaactaac agctttctca aagcggtgac cactcaggcc agcccagaca
     5761 aatctgaggg atggccagtg cactccaatg atgggacagg cctaacaaca catgtaagct
     5821 tccccgagag ctttcagctg gttcacctct ttgttctcta gactcttaag tactgactgc
     5881 tttgactttt gtgattatgt tatggtgatg tgtagtcagt gtaccaatat gttcacaacc
     5941 taggatcatg ataatggagt gtgttttggg ttttttttaa ctgttcagaa aaaaagtaaa
     6001 ttacaaatat aagattaaag tgaa
//
