LOCUS       NM_001207               1035 bp    mRNA    linear   PRI 30-APR-2025
DEFINITION  Homo sapiens basic transcription factor 3 (BTF3), transcript
            variant 2, mRNA.
ACCESSION   NM_001207
VERSION     NM_001207.5
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Cui,Y., Pu,M., Gong,Y., Li,R., Wang,X., Ye,J., Huang,H., Liao,D.,
            Yang,Y., Yin,A., Li,J., Deng,Y., Tian,Z. and Pu,R.
  TITLE     METTL3-driven m6A modification of lncRNA FAM230B suppresses
            ferroptosis by modulating miR-27a-5p/BTF3 axis in gastric cancer
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (11), 130714 (2024)
   PUBMED   39278369
  REMARK    GeneRIF: METTL3-driven m6A modification of lncRNA FAM230B
            suppresses ferroptosis by modulating miR-27a-5p/BTF3 axis in
            gastric cancer.
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Hu,M., Dai,C., Sun,X., Chen,Y., Xu,N., Lin,Z., Xu,S., Cheng,C.,
            Tan,Z., Bian,S. and Zheng,W.
  TITLE     Ubiquitination-specific protease 7 enhances stemness of
            hepatocellular carcinoma by stabilizing basic transcription factor
            3
  JOURNAL   Funct Integr Genomics 24 (1), 28 (2024)
   PUBMED   38340226
  REMARK    GeneRIF: Ubiquitination-specific protease 7 enhances stemness of
            hepatocellular carcinoma by stabilizing basic transcription factor
            3.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1035)
  AUTHORS   Wang,P., Sun,J., Sun,C., Zhao,H., Zhang,Y. and Chen,J.
  TITLE     BTF3 promotes proliferation and glycolysis in hepatocellular
            carcinoma by regulating GLUT1
  JOURNAL   Cancer Biol Ther 24 (1), 2225884 (2023)
   PUBMED   37382415
  REMARK    GeneRIF: BTF3 promotes proliferation and glycolysis in
            hepatocellular carcinoma by regulating GLUT1.
REFERENCE   4  (bases 1 to 1035)
  AUTHORS   Zhou,W., Yun,Z., Wang,T., Li,C. and Zhang,J.
  TITLE     BTF3-mediated regulation of BMI1 promotes colorectal cancer through
            influencing epithelial-mesenchymal transition and stem cell-like
            traits
  JOURNAL   Int J Biol Macromol 187, 800-810 (2021)
   PUBMED   34293363
  REMARK    GeneRIF: BTF3-mediated regulation of BMI1 promotes colorectal
            cancer through influencing epithelial-mesenchymal transition and
            stem cell-like traits.
REFERENCE   5  (bases 1 to 1035)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 1035)
  AUTHORS   Beatrix,B., Sakai,H. and Wiedmann,M.
  TITLE     The alpha and beta subunit of the nascent polypeptide-associated
            complex have distinct functions
  JOURNAL   J Biol Chem 275 (48), 37838-37845 (2000)
   PUBMED   10982809
REFERENCE   7  (bases 1 to 1035)
  AUTHORS   Grein,S. and Pyerin,W.
  TITLE     BTF3 is a potential new substrate of protein kinase CK2
  JOURNAL   Mol Cell Biochem 191 (1-2), 121-128 (1999)
   PUBMED   10094400
REFERENCE   8  (bases 1 to 1035)
  AUTHORS   Wiedmann,B., Sakai,H., Davis,T.A. and Wiedmann,M.
  TITLE     A protein complex required for signal-sequence-specific sorting and
            translocation
  JOURNAL   Nature 370 (6489), 434-440 (1994)
   PUBMED   8047162
REFERENCE   9  (bases 1 to 1035)
  AUTHORS   Kanno,M., Chalut,C. and Egly,J.M.
  TITLE     Genomic structure of the putative BTF3 transcription factor
  JOURNAL   Gene 117 (2), 219-228 (1992)
   PUBMED   1386332
REFERENCE   10 (bases 1 to 1035)
  AUTHORS   Zheng,X.M., Black,D., Chambon,P. and Egly,J.M.
  TITLE     Sequencing and expression of complementary DNA for the general
            transcription factor BTF3
  JOURNAL   Nature 344 (6266), 556-559 (1990)
   PUBMED   2320128
  REMARK    GeneRIF: In addition to RNA polymerase II (otherwise RNA pol II,
            RNA polymerase B), four general transcription factors are required
            for initiation of transcription: BTF1 (also referred to as TFIID)
            which has recently been cloned from yeast, BTF2, BTF3 and STF.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU661989.1, BC008062.2,
            BG182166.1 and AC099522.3.
            
            On Aug 13, 2020 this sequence version replaced NM_001207.4.
            
            Summary: This gene encodes the basic transcription factor 3. This
            protein forms a stable complex with RNA polymerase IIB and is
            required for transcriptional initiation. Alternative splicing
            results in multiple transcript variants encoding different
            isoforms. This gene has multiple pseudogenes. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1,
            resulting in translation initiation at a downstream AUG and a
            protein (isoform B) with a shorter N-terminus, compared to isoform
            A.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225964.1, CD048333.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BU661989.1         35-49
            16-863              BC008062.2         1-848
            864-991             BG182166.1         310-437
            992-1035            AC099522.3         14512-14555         c
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.2"
     gene            1..1035
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /note="basic transcription factor 3"
                     /db_xref="GeneID:689"
                     /db_xref="HGNC:HGNC:1125"
                     /db_xref="MIM:602542"
     exon            1..117
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..606
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /note="isoform B is encoded by transcript variant 2; RNA
                     polymerase B transcription factor 3; transcription factor
                     BTF3; nascent-polypeptide-associated complex beta
                     polypeptide; NAC-beta; nascent polypeptide-associated
                     complex subunit beta"
                     /codon_start=1
                     /product="transcription factor BTF3 isoform B"
                     /protein_id="NP_001198.2"
                     /db_xref="CCDS:CCDS4019.1"
                     /db_xref="GeneID:689"
                     /db_xref="HGNC:HGNC:1125"
                     /db_xref="MIM:602542"
                     /translation="MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKL
                     QFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEM
                     LPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNE
                     AN"
     exon            118..186
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /inference="alignment:Splign:2.1.0"
     exon            187..300
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /inference="alignment:Splign:2.1.0"
     exon            301..502
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /inference="alignment:Splign:2.1.0"
     exon            503..559
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /inference="alignment:Splign:2.1.0"
     exon            560..1035
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      843..848
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /note="hexamer: AATAAA"
     polyA_site      863
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /note="major polyA site"
     regulatory      973..978
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /note="hexamer: ATTAAA"
     polyA_site      990
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
     regulatory      1013..1018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
                     /note="hexamer: TATAAA"
     polyA_site      1035
                     /gene="BTF3"
                     /gene_synonym="BETA-NAC; BTF3a; BTF3b; NACB"
ORIGIN      
        1 ccctttagct gccatcttgc gtccccgcgt gtgtgcgcct aatctcaggt ggtccacccg
       61 agaccccttg agcaccaacc ctagtccccc gcgcggcccc ttattcgctc cgacaagatg
      121 aaagaaacaa tcatgaacca ggaaaaactc gccaaactgc aggcacaagt gcgcattggt
      181 gggaaaggaa ctgctcgcag aaagaagaag gtggttcata gaacagccac agcagatgac
      241 aaaaaacttc agttctcctt aaagaagtta ggggtaaaca atatctctgg tattgaagag
      301 gtgaatatgt ttacaaacca aggaacagtg atccacttta acaaccctaa agttcaggca
      361 tctctggcag cgaacacttt caccattaca ggccatgctg agacaaagca gctgacagaa
      421 atgctaccca gcatcttaaa ccagcttggt gcggatagtc tgactagttt aaggagactg
      481 gccgaagctc tgcccaaaca atctgtggat ggaaaagcac cacttgctac tggagaggat
      541 gatgatgatg aagttccaga tcttgtggag aattttgatg aggcttccaa gaatgaggca
      601 aactgaattg agtcaacttc tgaagataaa acctgaagaa gttactggga gctgctattt
      661 tatattatga ctgcttttta agaaattttt gtttatggat ctgataaaat ctagatctct
      721 aatattttta agcccaagcc ccttggacac tgcagctctt ttcagttttt gcttatacac
      781 aattcattct ttgcagctaa ttaagccgaa gaagcctggg aatcaagttt gaaacaaaga
      841 ttaataaagt tctttgccta gtatacagtt ttattttttt atttcattga caccgatctg
      901 tacacagtaa aaaaaattgc ttatagaaag ctaatcatgg catgtaatat ggctgataac
      961 ctttggaatt tgattaaaga tttaaaatca cggtgtagtg taacaaaggt ggtataaagt
     1021 tctcaggttt gaaaa
//
